WO2014088296A1 - Composition de blanchiment de la peau employant xylosma congesta - Google Patents

Composition de blanchiment de la peau employant xylosma congesta Download PDF

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Publication number
WO2014088296A1
WO2014088296A1 PCT/KR2013/011126 KR2013011126W WO2014088296A1 WO 2014088296 A1 WO2014088296 A1 WO 2014088296A1 KR 2013011126 W KR2013011126 W KR 2013011126W WO 2014088296 A1 WO2014088296 A1 WO 2014088296A1
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WO
WIPO (PCT)
Prior art keywords
extract
composition
skin whitening
powder
present
Prior art date
Application number
PCT/KR2013/011126
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English (en)
Korean (ko)
Inventor
오좌섭
안은경
고혜진
이재연
정용환
전아림
최경민
정미란
최미래
이도승
Original Assignee
재단법인 경기과학기술진흥원
재단법인 제주테크노파크
재단법인 진안홍삼연구소
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020130081127A external-priority patent/KR101568656B1/ko
Application filed by 재단법인 경기과학기술진흥원, 재단법인 제주테크노파크, 재단법인 진안홍삼연구소 filed Critical 재단법인 경기과학기술진흥원
Publication of WO2014088296A1 publication Critical patent/WO2014088296A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]

Definitions

  • the present invention relates to a composition for skin whitening using an extract of Xylosma congesta.
  • Melanin protects sub-dermal skin organs by blocking UV rays and protects the damage of proteins and genes in the skin caused by free radicals generated in the skin. It causes hyperpigmentation such as blemishes and freckles.
  • Melanin synthesis is achieved by the action of tyrosinase and tyrosinase related proteins present in the melanosomes. Specifically, tyrosine is oxidized by tyrosinase and is converted into dopa (DOPA, 3,4-dihydroxyphenylalanine), followed by oxidization of dopa by the action of dopaoxidase and then dopaquinone.
  • melanin is produced by the action of tyrosinase-related proteins TRP-1 (5,6-dihydroxy indole-2-carboxylic acid oxidase) and TRP-2 (dopachrome tautomerase).
  • Tyrosinase, TRP-1 and TRP-2 are promoted by the transcription factors microphthalmia-associated transcription factor (MITF), which is known to inhibit the expression of extracellular signal-regulated kinase (ERK) (DS). Kim, et al., J Cell Sci. 2003; 116: 1699-706).
  • MITF microphthalmia-associated transcription factor
  • ERK extracellular signal-regulated kinase
  • the present invention discloses an extract of citron oil having a tyrosinase inhibitory activity and a melanogenesis inhibitory activity in B16F10, a melanoma cell stimulated with ⁇ -MSH, which is a melanogenesis-stimulating agent.
  • the inventors of the present invention showed that 70% ethanol extract of citron oil leaves B16F10, which is a melanoma cell stimulated with ⁇ -MSH, which has mushroom tyrosinase inhibitory activity and is also a melanogenesis-stimulating agent. When treated, it was confirmed that it has a melanin production inhibitory activity in a concentration-dependent manner.
  • composition for skin whitening of the present invention is provided based on the results of these experiments, and is characterized by including an acid extract as an active ingredient.
  • acid oil extract refers to an acid oil leaf, stem, root, or mixture thereof, which is an extract, alcohol having 1 to 4 carbon atoms such as methanol, ethanol, acetone, ethyl acetate, chloroform, methylene chloride, water, or these It is understood as meaning including the extract obtained by extraction with a mixed solvent of and fractions fractionated with the solvents listed above.
  • the extraction method includes both a method of dipping the extraction target in the extraction solvent and a method of distilling the extraction target with the extraction solvent.
  • the extraction method by dipping may be applied to any method such as cold soaking, heating, ultrasonic, reflux.
  • extract preferably means that the extract is obtained by extraction (cold, hot water extract or distillation) with water, ethanol or a mixed solvent thereof, more preferably 70% to 90% Mean ethanol aqueous solution.
  • % is a concentration (v / v) expression by volume percentage, and means a volume of solute dissolved in a volume of solution as known in the art. For example, 30% (v / v) means that 30 ml of solute is dissolved in 100 ml of the solution.
  • extract includes an extract in a form purified through filtration, a concentrated liquid extract from which some of the extraction solvent is removed, and a powdery extract from which the extraction solvent is completely removed.
  • active ingredient means a component that can exhibit activity alone or in combination with a carrier having no activity in itself.
  • skin whitening is understood as a result of the inhibition of the production of melanin, specifically, means the prevention, delayed expression or treatment of symptoms caused by the excessive production of melanin, such as blemishes, freckles, blotch, etc. .
  • the skin whitening composition of the present invention may further include other known compounds or plant extracts that are known to have a skin whitening effect so as to enhance or enhance the skin whitening effect.
  • the other compounds or plant extracts include, for example, mercaptosuccinic acid, mercaptodextran, tefrenone, dihydroxy-isoquinoline, 3-hydroxymanul, vitamin K, thiazolidone, kynurenine, lemon extract, cucumber Extracts, mulberry extracts, licorice extracts, rosemary extracts, acerola cherry extracts, ginkgo extracts, carob (carob) extracts, geranium extracts, herbal extracts, and the like, but is not limited thereto.
  • Such compounds or plant extracts may be included in the composition for skin whitening of the present invention with one or more active ingredients thereof.
  • the active ingredient may be included in any amount within a range that does not toxic to the human body depending on the use, formulation (cosmetic, soap, ointment, etc.), specifically, the whole composition It may be included in an amount of 0.01% to 99.99% by weight based on the weight.
  • the skin whitening composition of the present invention can be regarded as a cosmetic composition in a specific embodiment.
  • the cosmetic composition may be prepared in various formulations, such as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Contains cleansing, oils, powder found
  • Emulsion, emulsion foundation, wax foundation, spray, and the like but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion (skin), nutrition lotion (milk lotion), nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder .
  • the cosmetic composition of the present invention is prepared by including a suitable carrier component according to the dosage form, and in addition to the active ingredient, conventionally used ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. It may be prepared including an adjuvant.
  • Auxiliaries such as stabilizers, solubilizers, vitamins, fragrances, carrier components, etc. according to the formulation are known in the art, it can be selected and used appropriate materials.
  • the formulation of the cosmetic composition is a cream or a paste
  • animal fats such as larch, sunny, and fish oils
  • vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, wax, paraffin, starch and the like
  • the formulation of the composition is a soap
  • the vegetable or animal fats and oils exemplified above can be used as the carrier component.
  • lactose, talc, silica, aluminum hydroxide, or the like may be used as a carrier component, and in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether. have.
  • the skin lightening composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.
  • the pharmacological effect may be understood as a therapeutic or prophylactic effect for a disease resulting from an increase in melanin, and the meaning of the skin whitening is the treatment or It can be understood as the meaning of prevention.
  • diseases resulting from an increase in melanin include skin hyperpigmentation such as blemishes, freckles and blotch, and skin aging.
  • compositions of the present invention in addition to its active ingredients, include pharmaceutically acceptable carriers, excipients, and the like, oral formulations (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral formulations (sterile injectable aqueous solutions). Or oily suspensions), topical formulations (solutions, creams, ointments, gels, lotions, patches) and the like.
  • pharmaceutically acceptable means that the subject of application (prescription) is not as toxic as applicable, without inhibiting the activity of the active ingredient.
  • Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, solids Lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, and the like.
  • lactose glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, solids Lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut
  • emulsifiers such as TWEENS
  • wetting agents such as sodium lauryl sulfate
  • colorants such as sodium lauryl sulfate
  • flavors such as sodium lauryl sulfate
  • antioxidants such as sodium lauryl sulfate
  • preservatives water, saline, phosphate buffer solutions, and the like.
  • Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, when the pharmaceutical composition of the present invention is prepared with an aqueous suspending agent, suitable excipients are sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethylcellulose And sodium alginate, polyvinylpyrrolidone and the like. Suitable excipients when prepared as injections include Ringer's solution, isotonic sodium chloride, and the like.
  • compositions of the present invention may be administered orally or parenterally, but will typically be administered directly to the skin topically in a topical formulation.
  • the daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ⁇ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage limits the scope of the present invention in any aspect. It should not be understood.
  • composition of this invention can be grasped
  • the food composition of the present invention may be prepared as a dietary supplement, a special nutritional supplement, a functional beverage, and the like.
  • the food composition of the present invention may include sweeteners, flavoring agents, bioactive ingredients, minerals, etc. in addition to the active ingredients.
  • Sweeteners may be used in amounts that give the food a suitable sweet taste, and may be natural or synthetic.
  • a natural sweetener is used.
  • sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.
  • Flavoring agents can be used to enhance the taste or aroma, both natural and synthetic. It is the case of using a natural thing preferably.
  • the natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like.
  • ginseng red ginseng
  • bamboo shoots aloe vera, ginkgo and the like can be used.
  • Natural flavors can be liquid concentrates or solid extracts.
  • synthetic flavoring agents may be used, and synthetic flavoring agents may include esters, alcohols, aldehydes, terpenes, and the like.
  • catechins such as catechin, epicatechin, gallocatechin, epigallocatechin, vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, riboflavin, and the like can be used.
  • mineral calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc and the like can be used.
  • the food composition of the present invention may contain a preservative, an emulsifier, an acidulant, a thickener, and the like, in addition to the sweetener.
  • Such preservatives, emulsifiers and the like are preferably added and used in very small amounts as long as the use to which they are added can be achieved.
  • trace amount is meant numerically expressed in the range of 0.0005% to about 0.5% by weight based on the total weight of the food composition.
  • preservatives examples include sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), and the like.
  • Emulsifiers that can be used include acacia gum, carboxymethylcellulose, xanthan gum, pectin and the like.
  • acidulants examples include lead acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like. Such acidulant may be added so that the food composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing taste.
  • Thickeners that can be used include suspending implements, sedimenters, gel formers, swelling agents and the like.
  • the food composition of the present invention may include powders or extracts derived from natural products in order to enhance flavor and palatability and to enhance and enhance skin related functions such as wrinkle improvement, sun powder or extracts, bean sprout powder or extracts, shellfish powder or Extract, oyster powder or extract, hawthorn powder or extract, radish juice or extract, cucumber juice or extract, leek juice or extract, spinach juice or extract, lotus root juice or extract, sesame juice or extract, pine needle juice or extract, ginseng juice Or extracts, baekryechosa powder or extracts, licorice powder or extracts, browning powder or extracts, reed powder or extracts, sine powder or extracts, gourd powder or extracts, ginger powder or extracts, jujube powder or extracts, phosphorus powder or extracts, strips Purple Powder or Extract, Numerical Powder or Extract, White Extract Horse or extract, age powder or extract, tangerine seed (peel of tangerine) powder or extract, wolfberry powder or extract, green tea
  • the present invention can provide a composition for skin whitening using an acid extract.
  • the skin whitening composition of the present invention may be commercialized into cosmetics, drugs, foods and the like.
  • 1 is a result showing the melanin production inhibitory activity of the oleander leaf extract in B16F10, a melanoma cell stimulated with a melanogenesis-stimulating agent ⁇ -MSH.
  • Inhibition% 100- (A-B) / (C-D) x100
  • B16 / F10 cells were obtained from Korean Cell Line Bank (KCLB) as a melanoma cell line in rats. Incubated in 37 ° C., 5% CO 2 incubator using RPMI 1640 or DMEM medium (GIBCO, Grand Island, NY, USA) containing Grand Island, NY, USA, subcultures every 3-4 days was implemented.
  • KCLB Korean Cell Line Bank
  • B16F10 melanoma cell line was dispensed into 6 well plates at a cell number of 1 ⁇ 10 5 / well, and after 24 hours, the medium was removed, and 100 nM of ⁇ -melanocyte stimulating hormone ( ⁇ -MSH) and the positive control group arbutin and samples were treated by concentration. Incubate 72 hours later. After washing twice with PBS, lysis buffer (100 mM sodium phosphate (pH6.8), 0.1 mM PMSF, 1% Triton X-100) was added and dissolved at -80 ° C for 30 minutes. After cell collection, pellet was dissolved by reacting with 1N NaOH containing 10% DMSO at 65 ° C. for 1 hour and absorbance was measured at 405 nm.
  • ⁇ -MSH ⁇ -melanocyte stimulating hormone
  • the acid oil sample of the example showed melanin production inhibitory activity in proportion to the treatment concentration, and melanin production inhibitory activity was much superior to that of the positive control group Arbutin.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
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  • Mycology (AREA)
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  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une composition de blanchiment de la peau employant un extrait de Xylosma congesta qui a une activité inhibitrice sur une tyrosinase de champignon, et présente une activité inhibitrice sur la production de mélanine lorsqu'elle est traitée dans B16F10, qui est une cellule de mélanome stimulée par du α-MSH, qui est un stimulateur de mélanocyte.
PCT/KR2013/011126 2012-12-04 2013-12-03 Composition de blanchiment de la peau employant xylosma congesta WO2014088296A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20120139561 2012-12-04
KR10-2012-0139561 2012-12-04
KR1020130081127A KR101568656B1 (ko) 2012-12-04 2013-07-10 산유자 추출물을 이용한 피부 미백용 조성물
KR10-2013-0081127 2013-07-10

Publications (1)

Publication Number Publication Date
WO2014088296A1 true WO2014088296A1 (fr) 2014-06-12

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PCT/KR2013/011126 WO2014088296A1 (fr) 2012-12-04 2013-12-03 Composition de blanchiment de la peau employant xylosma congesta

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004045573A1 (fr) * 2002-11-19 2004-06-03 Doosan Corporation Composition a base de n-acetylphytosphingosine pour blanchiment de la peau
US20100055059A1 (en) * 2007-01-16 2010-03-04 Laboratoires Mayoly Spindler Association of compounds inhibiting melanogenesis and use thereof in cosmetics and dermatology
KR20100131058A (ko) * 2009-06-05 2010-12-15 중앙대학교 산학협력단 Ｆｔｙ720을 유효성분으로 포함하는 미백용 조성물
KR20110092398A (ko) * 2010-02-09 2011-08-18 재단법인 제주테크노파크 섬오갈피 근피 성분인 아칸토산을 이용한 피부 미백제 조성물
KR101174786B1 (ko) * 2010-04-07 2012-08-17 한국메카투라주식회사 피부미용 수분민감성 미세바늘패치 및 그 제조방법
KR20130125074A (ko) * 2012-05-08 2013-11-18 재단법인 경기과학기술진흥원 산유자 추출물을 이용한 항산화용 조성물

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004045573A1 (fr) * 2002-11-19 2004-06-03 Doosan Corporation Composition a base de n-acetylphytosphingosine pour blanchiment de la peau
US20100055059A1 (en) * 2007-01-16 2010-03-04 Laboratoires Mayoly Spindler Association of compounds inhibiting melanogenesis and use thereof in cosmetics and dermatology
KR20100131058A (ko) * 2009-06-05 2010-12-15 중앙대학교 산학협력단 Ｆｔｙ720을 유효성분으로 포함하는 미백용 조성물
KR20110092398A (ko) * 2010-02-09 2011-08-18 재단법인 제주테크노파크 섬오갈피 근피 성분인 아칸토산을 이용한 피부 미백제 조성물
KR101174786B1 (ko) * 2010-04-07 2012-08-17 한국메카투라주식회사 피부미용 수분민감성 미세바늘패치 및 그 제조방법
KR20130125074A (ko) * 2012-05-08 2013-11-18 재단법인 경기과학기술진흥원 산유자 추출물을 이용한 항산화용 조성물

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