WO2014025234A3 - 암 줄기세포에 특이적으로 결합하는 핵산 앱타머 및 이의 용도 - Google Patents

암 줄기세포에 특이적으로 결합하는 핵산 앱타머 및 이의 용도 Download PDF

Info

Publication number
WO2014025234A3
WO2014025234A3 PCT/KR2013/007212 KR2013007212W WO2014025234A3 WO 2014025234 A3 WO2014025234 A3 WO 2014025234A3 KR 2013007212 W KR2013007212 W KR 2013007212W WO 2014025234 A3 WO2014025234 A3 WO 2014025234A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid aptamers
pancreatic cancer
stem cells
present
Prior art date
Application number
PCT/KR2013/007212
Other languages
English (en)
French (fr)
Other versions
WO2014025234A2 (ko
Inventor
송시영
김윤진
Original Assignee
연세대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 연세대학교 산학협력단 filed Critical 연세대학교 산학협력단
Publication of WO2014025234A2 publication Critical patent/WO2014025234A2/ko
Publication of WO2014025234A3 publication Critical patent/WO2014025234A3/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 서열목록 제1서열 내지 제7서열로 구성된 군으로부터 선택되는 뉴클레오타이드 서열을 포함하는 핵산 앱타머와 이의 용도에 관한 것이다. 본 발명의 핵산 앱타머는 암 세포 특히 췌장암 암 줄기세포에 특이적으로 결합하는 특성을 갖는다. 따라서, 본 발명의 핵산 앱타머는 암의 진단 및 표적뿐만 아니라, 췌장암의 진단 또는 예후 판단 용도로 사용될 수 있다. 본 발명의 DNA 앱타머는 췌장암 진단에 활용되고 있는 췌장암 결합 항체를 대체할 수 있으며, 췌장암을 보다 신속하고 경제적으로 진단할 수 있을 것으로 기대된다.
PCT/KR2013/007212 2012-08-09 2013-08-09 암 줄기세포에 특이적으로 결합하는 핵산 앱타머 및 이의 용도 WO2014025234A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20120087272 2012-08-09
KR10-2012-0087272 2012-08-09

Publications (2)

Publication Number Publication Date
WO2014025234A2 WO2014025234A2 (ko) 2014-02-13
WO2014025234A3 true WO2014025234A3 (ko) 2014-04-03

Family

ID=50068660

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2013/007212 WO2014025234A2 (ko) 2012-08-09 2013-08-09 암 줄기세포에 특이적으로 결합하는 핵산 앱타머 및 이의 용도

Country Status (2)

Country Link
KR (1) KR101568400B1 (ko)
WO (1) WO2014025234A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108866064B (zh) * 2018-07-10 2022-05-06 中国医科大学 一种靶向转移性人乳腺癌细胞的核酸适配体及其应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020130225A1 (ko) * 2018-12-21 2020-06-25 국립암센터 췌장암 특이적으로 결합하는 dna 앱타머 및 이의 용도
KR102212149B1 (ko) * 2019-03-28 2021-02-04 부산대학교 산학협력단 암 줄기세포에 특이적으로 결합하는 압타머 및 이의 용도

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080132423A1 (en) * 2004-11-12 2008-06-05 Toru Kondo Methods And Means Related To Cancer Stem Cells
US20090202564A1 (en) * 2006-05-02 2009-08-13 Stelic Institute Of Regenerative Medicine, Stelic Institute & Co. Methods of isolating stem cells
KR20100129709A (ko) * 2009-06-01 2010-12-09 성균관대학교산학협력단 췌장암 세포 또는 조직에 특이적으로 결합할 수 있는 핵산 압타머 및 그 용도
US20110184042A1 (en) * 2009-03-17 2011-07-28 Postech Academy-Industry Foundation RNA Aptamer Specifically Binding to Carcinoembryonic Antigen and Use thereof
KR20110086433A (ko) * 2010-01-22 2011-07-28 성균관대학교산학협력단 Ｈｅｒ－２ 과발현 유방암 세포 또는 조직에 특이적으로 결합할 수 있는 핵산 압타머 및 그 용도

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080132423A1 (en) * 2004-11-12 2008-06-05 Toru Kondo Methods And Means Related To Cancer Stem Cells
US20090202564A1 (en) * 2006-05-02 2009-08-13 Stelic Institute Of Regenerative Medicine, Stelic Institute & Co. Methods of isolating stem cells
US20110184042A1 (en) * 2009-03-17 2011-07-28 Postech Academy-Industry Foundation RNA Aptamer Specifically Binding to Carcinoembryonic Antigen and Use thereof
KR20100129709A (ko) * 2009-06-01 2010-12-09 성균관대학교산학협력단 췌장암 세포 또는 조직에 특이적으로 결합할 수 있는 핵산 압타머 및 그 용도
KR20110086433A (ko) * 2010-01-22 2011-07-28 성균관대학교산학협력단 Ｈｅｒ－２ 과발현 유방암 세포 또는 조직에 특이적으로 결합할 수 있는 핵산 압타머 및 그 용도

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SHIGDAR, SARAH ET AL.: "RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule", CANCER SCIENCE, vol. 102, no. 5, May 2011 (2011-05-01), pages 991 - 998 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108866064B (zh) * 2018-07-10 2022-05-06 中国医科大学 一种靶向转移性人乳腺癌细胞的核酸适配体及其应用

Also Published As

Publication number Publication date
KR20140021973A (ko) 2014-02-21
WO2014025234A2 (ko) 2014-02-13
KR101568400B1 (ko) 2015-11-12

Similar Documents

Publication Publication Date Title
MX338063B (es) Molecula de union especifica al rsv.
WO2011079902A3 (en) Biological inhibitors of ror1 capable of inducing cell death
WO2012083969A3 (en) Microrna for diagnosis of pancreatic cancer
WO2013032242A3 (ko) 그래핀을 이용한 타겟 비고정화 방식의 앱타머 선별방법 및 이로부터 선별된 앱타머
WO2012134602A3 (en) Methods and systems for sequencing long nucleic acids
WO2013104804A3 (en) Dual antigen-induced bipartite functional complementation
WO2011133886A3 (en) Production of heteromultimeric proteins
MX2010008099A (es) Anticuerpos y angiopoyetina-2 estabilizados y sus usos.
WO2011015944A3 (en) Use of free dna as an early predictor of severity in acute pancreatitis
WO2012160563A3 (en) Use of akt phosphorylation as a biomarker for prognosing neurodegenerative diseases and treating same
WO2006135527A3 (en) Methods for the selection of aptamers
WO2010115745A3 (en) Biomarkers, methods and kits for the diagnosis of rheumatoid arthritis
EP2473631A4 (en) BINDING-INDUCED HAIR NEEDLE DETECTION SYSTEM
WO2010087594A3 (ko) 시디９３ 또는 이의 가용성 단편의 용도
WO2012155134A3 (en) Assay reagents for a neurogranin diagnostic kit
WO2013176992A3 (en) Methods and compositions for the diagnosis of sepsis using gamma peptide nucleic acids
WO2014025234A3 (ko) 암 줄기세포에 특이적으로 결합하는 핵산 앱타머 및 이의 용도
WO2012021493A3 (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
GB201021499D0 (en) Detection of quantative genetic differnces
WO2012112958A3 (en) High-throughput assays to probe leukemia within the stromal niche
WO2014152091A3 (en) Methods of genome sequencing and epigenetic analysis
WO2013093315A3 (fr) Procede pour le diagnostic ou le pronostic, in vitro, du cancer du colon
WO2012135620A8 (en) Molecular gram stain
WO2013093347A3 (fr) Procede pour le diagnostic ou le pronostic, in vitro, du cancer de l'ovaire
WO2009090269A3 (en) Markers and diagnostic methods for metastasis

Legal Events

Date Code Title Description
122 Ep: pct application non-entry in european phase

Ref document number: 13827376

Country of ref document: EP

Kind code of ref document: A2