WO2014013068A1 - Combinaison pour réduire les émissions de méthane dans le fourrage de ruminants - Google Patents

Combinaison pour réduire les émissions de méthane dans le fourrage de ruminants Download PDF

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Publication number
WO2014013068A1
WO2014013068A1 PCT/EP2013/065338 EP2013065338W WO2014013068A1 WO 2014013068 A1 WO2014013068 A1 WO 2014013068A1 EP 2013065338 W EP2013065338 W EP 2013065338W WO 2014013068 A1 WO2014013068 A1 WO 2014013068A1
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acid
weight
components
component
composition
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PCT/EP2013/065338
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German (de)
English (en)
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Bernhard ECKEL
Elisabeth HOLL
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Dr. Eckel Gmbh
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/22—Methane [CH4], e.g. from rice paddies

Definitions

  • the invention relates to the use of a combination comprising one or more saponin-containing plant extracts and / or plant components (component A), one or more antimicrobial and / or flavonoid-containing plant extracts and / or plant components (component B), and one or more substances selected from the group consisting of the (CrC 8 ) monocarboxylic acids, (C 2 -C 8 ) dicarboxylic acids and (C 3 -C 8 ) tricarboxylic acids, and their sodium, potassium, calcium and magnesium salts (component C) for Reduction of methane emissions from ruminant animals.
  • component A saponin-containing plant extracts and / or plant components
  • component B one or more antimicrobial and / or flavonoid-containing plant extracts and / or plant components
  • component C the group consisting of the (CrC 8 ) monocarboxylic acids, (C 2 -C 8 ) dicarboxylic acids and (C 3 -C 8 ) tric
  • Methane is a colorless and odorless gas that burns with a bluish flame.
  • Environmental inputs are the result of natural formation in the anaerobic degradation of organic substances, such as celluloses and hemicelluloses. These are fermented in ruminants, but also in camelids by anaerobic bacteria, the methanogenic archaebacteria.
  • Methane is one of the most important greenhouse gases.
  • the relative effectiveness of methane as a greenhouse gas is reflected in its associated global warming potential (GWP) of about 25. That is, the effect of 1 g of methane corresponds to that of 23 g of carbon dioxide. 12% of global methane emissions are caused by ruminants.
  • GWP global warming potential
  • Ruminants are herbivores, which are characterized by the fact that they have a Herhöhligen stomach. This consists of the three Vorwenagen rumen, hood and Blattmagen, which are the actual glandular stomach (abomasum) upstream. This gastric system, also called rumen, is characterized by the fact that it can decompose by microbial conversion, in contrast to animals with einhöhligem stomach, even difficult to digest fibrous and structural substances such as celluloses and hemicelluloses.
  • camels (Camelidae) are not zoologically counted as subordinates of ruminants, they have, like ruminants, a multi-chambered stomach which is used for better digestion of pure vegetable food but has developed independently of the stomach of ruminants.
  • the rumen can be described as a large fermentation chamber, in which bacteria, yeasts and protozoa break down the plants taken in with the porridge and partly Rebuild nutrients.
  • bacteria, yeasts and protozoa break down the plants taken in with the porridge and partly Rebuild nutrients.
  • rumen about 200 species of bacteria ( ⁇ 10 10 / ml rumen fluid), about 25 genera of protozoa (10 6 / ml rumen fluid) and 5 genera of anaerobic fungi have been detected, which correspond to about 8% of the ruminal biomass.
  • archaebacteria are found that are methanogenic.
  • the ruminal methane formation (methanogenesis) is very closely linked to the microbial fermentation of carbohydrates in the rumen. Methane formation can be considered as a consequence of the transfer of metabolic hydrogen. For example, the stoichiometry of the fermentation of hexose to the three most important volatile fatty acids is shown below:
  • the hydrogen formed inhibits the rumen fermentation and must therefore be continuously removed from the system.
  • This hydrogen released from the microbial conversion is used by methane bacteria, such as Methanobacterium ruminantium and Methanobacterium mobilis, to reduce C0 2 to methane: 4 H 2 + C0 2 -> CH 4 + 2 H 2 0
  • methanogenesis is thus the removal of H 2 excess. This is physiologically important, but leads to a significant environmental impact. So up to 500 liters of methane per ruminant and day can be formed. Each year, one cow emits up to 143 kg of methane, which corresponds to 3290 kg C0 2 equivalent. This means that about 65% of the excreted C0 2 equivalents of a cow are due to methane emissions. In addition to the pure environmental aspect, the methane emissions mean another disadvantage for ruminants. Approximately 2 to 20% of the gross energy absorbed is excreted via methane and thus not available to the animal.
  • ruminants are fed with crude fiber-rich rations, which are converted by microorganisms.
  • the rumen is optimally adapted for this purpose, in contrast to animals with a cavernous stomach.
  • the methanogenesis can be sustainably lowered. This is due to the altered substrates for the fermentation and a concomitant lowering of the rumen pH.
  • the performance of the animals can be increased, which also leads to a reduction in the relative methane production per kilogram of animal protein produced.
  • this method contradicts a species-appropriate ruminant feeding.
  • the potential of the rumen is not exhausted.
  • Another aspect that militates against this process is the fact that, in the context of this ration change, ruminants are in direct food competition with humans. Furthermore, it should be noted that the milk quality can be negatively influenced by this feeding process.
  • Another method described for reducing methane emissions is the use of fats.
  • fats In normal ruminant feeding a maximum of 6 to 7% fat in the total dry matter intake are used for nutritional reasons. The mechanism of action is also associated with a change in the microflora in this case.
  • three changes in the literature are known to be described (K. A. Johnson, D.E. Johnson, J. Anim., Sci 1995, 73: 2483-2492).
  • the fermentation of the organic substance and at the same time the number of methanogenic bacteria and protozoa are reduced.
  • feeding unsaturated fatty acids by hydrogenating them reduces methane formation by providing alternative hydrogen acceptors.
  • this method has the disadvantage that it depends on several factors, e.g. from the fat sources and fatty acid patterns that are being fed. Also, the type of ration is known to have a decisive impact on success.
  • ionophoric substances are usually macro-cyclic compounds, the molecular weight is generally below 2000.
  • naturally occurring ionophores include macrolides, peptide antibiotics and polyether antibiotics. These substances are used to optimize feed intake and improve efficiency in milk and meat production in ruminants. However, these are banned in the European Union for this use. It is believed that the effect of the ionophore substances is based on reducing the type of protozoa typical of the rumen and improving the acetic acid to propionic acid ratio by reducing the formation of propionic acid (KA Beauchemin et al., Aust. J. of Exp.
  • WO201 1/070133 describes that the use of nitrooxyalkanoic acids and their derivatives can reduce the methane emissions of ruminants.
  • Another possibility for reducing methane production is the use of certain organic acids, in particular the dicarboxylic acids (S.A. Martin, J. Anim., Vol. 1998, 76: 3123-3132).
  • dicarboxylic acids S.A. Martin, J. Anim., Vol. 1998, 76: 3123-3132.
  • no such effects have previously been described for short-chain, volatile monocarboxylic acids.
  • the object of the present invention is therefore to provide compositions or combinations which can bring about a reduction of methane emissions in ruminants and camelids.
  • the above object has been achieved by the use of a combination comprising one or more saponin components, in particular one or more saponin-containing plant extracts and / or plant components (component (A)), one or more antimicrobial components and / or one or more flavonoid components.
  • component (A) saponin-containing plant extracts and / or plant components
  • component (A) one or more antimicrobial components and / or one or more flavonoid components.
  • Components in particular one or more antimicrobial and / or flavonoid-containing plant extracts and / or plant components (component (B)), and one or more substances selected from the group consisting of the (dC 8 ) monocarboxylic acids, (C 2 -C 8 ) - Dicarboxylic acids and (C 3 -C 8 ) tricarboxylic acids, and their sodium, potassium, calcium and magnesium salts (component (C)) to reduce the methane emissions of ruminant animals.
  • component (B) Component (B)
  • An object of the present invention is therefore the use of a combination comprising the components (A), (B) and (C):
  • An object of the present invention is therefore in particular also the use of a combination comprising the components (A), (B) and (C):
  • the feeding of the combination according to the invention also has a pH-stabilizing, in particular pH and redox potential-lowering effect on the rumen environment.
  • Another object of the present invention is therefore also the use of a combination according to the invention comprising components (A), (B) and (C) for reducing subacute acidosis in ruminants.
  • FIG. 1 shows the effect of an inventive use of the combination on the pH of the rumen environment on the basis of in vitro examinations (RUSITEC system).
  • FIG. 2 shows the effect of an inventive use of the combination on the redox potential of the rumen environment on the basis of in vitro examinations (RUSITEC system).
  • FIG. 3 shows the effect of an inventive use of the combination on the methane content in the rumen on the basis of in vitro examinations (RUSITEC system).
  • ruminant animals in the context of the invention includes herbivores that have a multi-hollow stomach and can digest by microbial conversion and difficult to digest fibrous and structural substances such as celluloses and hemicelluloses.
  • ruminant animals includes in particular all animals of the suborder of ruminants (Ruminantiä), for example the animals of the family of the bovidae (Bovidae), comprising the animals of the genera Capra (goats), Ovis (sheep), Bos (cattle) and the antelopes; as well as the ruminant-like animals of the family Camelidae (camels).
  • the Camels family includes the Old World Camels (Camelus), which includes the Dromedaries (Crab Drummed Crabs, C. dromedarius) and Bactrian Crabs (Two-Bumped Camels, C. bactrianus), and the New World Camels, which include the Guanacos (L guanicoe), Lamas (L glam) , Alpacas (L pacos) and Vikunjas (V. vicugnä).
  • the components used according to the invention can be of natural or synthetic origin or derived from natural materials, for example by derivatization. If, in the combination according to the invention, the components used are used, for example, in the form of plant extracts or plant constituents, it is known to the person skilled in the art how these components can be obtained, in particular in such a form that they meet the requirements for use in feeding Animals are made. For example, see the Community Register of Feed Additives as an integral part of Regulation (EC) No 1831/2003 Appendixes 3 & 4. Annex: List of additives (Status: Released April 23, 2009) [Rev. 48] or the version No 1831/2003 Appendixes 3c & 4. Annex: List of additives (Status: Released 17 July 2013).
  • the combination contains as component (A) one or more saponin-containing plant extracts and / or plant components.
  • Saponins are glycosides of steroids (steroid saponins), steroid alkaloids (steroid alkaloid saponins) or triterpenes (triterpene saponins).
  • the term "saponins” comes from the fact that these compounds have surface-active properties, so that when shaking with water, a soap-like foam is formed.
  • Particularly rich in saponins, in particular triterpene saponins, is the bark of the soap bark tree (Quillaja saponariä), also called panamarinde. It contains up to 10% by weight of saponins.
  • component (A) comprises one or more extracts and / or constituents of at least one plant selected from the group consisting of Yucca Schidigera, Sapindus Saponaria, Quillaja Saponaria, Ginseng (Panax Ginseng), Sweetwood (Glycyrrhiza Glabra), Quinoa (Chenopodium Quinoa ) and clover.
  • the combination contains as component (B) one or more antimicrobial and / or flavonoid-containing plant extracts and / or plant components.
  • antimicrobial plant extracts and / or plant constituents for the purposes of the invention includes all herbal preparations, products and extracts which have an antimicrobial effect.
  • Antimicrobial extracts or preparations can be obtained, for example, from oregano, hops, rosemary, grape pomace, grape seeds, garlic, buckwheat or Hibiscus sabdariffa. These plants are all rich in flavonoids.
  • component (B) Preferably as component (B) one or more extracts and / or plant components selected from the group consisting of grape pomace, grape seed extract and the extracts and plant components of hops, oregano, rosemary, garlic, buckwheat (Fagopyrum esculentum) and Roselle (Hibiscus sabdariffa) in front.
  • component (B) one or more extracts and / or plant components selected from the group consisting of grape pomace, grape seed extract and the extracts and plant components of hops, oregano, rosemary, garlic, buckwheat (Fagopyrum esculentum) and Roselle (Hibiscus sabdariffa) in front.
  • the inflorescences of the female plant optionally in pelleted form, hop extract, extract hop pellets or hop grains are preferably used.
  • These contain u.a. the prenylated flavonoid xanthohumol, as well as catechins, epicatechins, proanthocyanidins, glycosidically linked quercetin and kaempferol, as well as catechols and tannins.
  • hop grains in the context of the invention comprises the residues which remain as by-products during brewing as insoluble constituents in hop cones, and in particular include parts of the leaves and stems of the hop.
  • hop grains includes the extraction residues from hop extraction. In the case of oregano and rosemary, all preparations and extracts obtained from the leaves are particularly suitable. These include the flavonoids luteolin and apigenin.
  • garlic for the purposes of the invention includes all products, preparations and extracts obtained from the roots of the garlic plant. These have a high content of flavonoids, in particular quercetin.
  • buckwheat preparations of the leaves are preferably used as vegetable parts of buckwheat preparations of the leaves.
  • the seed coat of buckwheat is rich in flavonoids, especially rutin, orientin, isoorientin, vitexin and isovitexin.
  • Hibiscus sabdariffa (Roselle) for the purposes of the invention includes all products and preparations that can be obtained from the plant, including all components of the Hibiscus sabdariffa PWanze, in particular the seeds, leaves, fruits and roots, and the obtained therefrom Extracts
  • Preparations derived from the calyx are especially preferred, and include in particular the flavonoids gossypetine, hibiscetin and sabdaretin.
  • the term "grape pomace” comprises the predominantly solid residues which remain as a by-product in the extraction of wine after the juice has been squeezed out, and in particular include the hides, stems, kernels and pulp of the grapes.
  • the content of flavonoids is dependent on the batch in the range between 0.1 wt .-% and 8 wt .-%.
  • anthocyanins e.g. Cyanidin, delphinidin and pelargonidin
  • Flavonoids e.g. Myricetin, quercetin, fighter egg and catechin
  • oligomeric proanthocyanidins oligomeric proanthocyanidins.
  • the combination contains as component (C) one or more substances selected from the group consisting of the (CrC 8 ) monocarboxylic acids, (C 2 -C 8 ) dicarboxylic acids and (C 3 -C 8 ) tricarboxylic acids, and their sodium , Potassium, calcium and magnesium salts.
  • component (C) one or more substances selected from the group consisting of the (CrC 8 ) monocarboxylic acids, (C 2 -C 8 ) dicarboxylic acids and (C 3 -C 8 ) tricarboxylic acids, and their sodium , Potassium, calcium and magnesium salts.
  • di- or tricarboxylic acids split off two or three protons and can therefore form two or three salt rows.
  • the sodium salts monosodium citrate, disodium citrate and trisodium citrate derive from the tri-citric acid.
  • component (C) are one or more substances selected from the group consisting of formic acid, acetic acid, lactic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, pivalic acid, salicylic acid, benzoic acid, nicotinic acid, bile acid, oxalic acid, malonic acid, glyoxylic acid, fumaric acid , Malic, succinic, aspartic, tartaric, maleic, glutaric, a-ketoglutaric, adipic and citric acids, and their sodium, potassium, calcium and magnesium salts.
  • component (C) is at least one substance selected from the group consisting of formic acid, acetic acid, propionic acid, butyric acid, fumaric acid, malic acid, tartaric acid and citric acid, and their sodium, potassium, calcium and magnesium salts.
  • component (C) at least one (CC 8 ) monocarboxylic acid, preferably selected from the group consisting of formic acid, acetic acid, propionic acid, butyric acid and / or at least one of its sodium, potassium, calcium and magnesium salts, in front.
  • component (C) at least one (CC 8 ) monocarboxylic acid, preferably selected from the group consisting of formic acid, acetic acid, propionic acid, butyric acid and / or at least one of its sodium, potassium, calcium and magnesium salts, in front.
  • At least one salt of a (CC 8 ) monocarboxylic acid preferably selected from the group consisting of the sodium, potassium, calcium and magnesium salts of formic acid, acetic acid, propionic acid and butyric acid, is present as component (C).
  • Particularly preferred are the sodium, calcium and magnesium salts of acetic acid and propionic acid.
  • the combination comprises at least one salt of a (CC 8 ) -monocarboxylic acid, preferably selected from the group comprising the sodium, potassium, calcium and magnesium salts of acetic acid and propionic acid, and at least one (C 2 -) C 8 ) di- or (C 3 -C 8 ) -tricarboxylic acid, preferably selected from the group consisting of fumaric acid, malic acid, tartaric acid and citric acid and their sodium, potassium, calcium and magnesium salts as component (C).
  • a (CC 8 ) -monocarboxylic acid preferably selected from the group comprising the sodium, potassium, calcium and magnesium salts of acetic acid and propionic acid
  • C 2 -) C 8 di- or (C 3 -C 8 ) -tricarboxylic acid
  • the combination is used to reduce methane emissions from ruminating animals.
  • the components (A), (B) and (C) can be administered orally to the ruminating animals individually, ie separately, and / or in the form of a premix and / or via the complete feed.
  • the premix may comprise two of the components (A), (B) and (C) in any combination or all three components. If the premix comprises only two of components (A), (B) and (C), the third component may be administered to the animals individually or in the form of another premix. For example, the animals may be administered a premix containing components (A) and (C) and component (B) individually, or component (B) is further incorporated in the form of a second premix containing a second portion of component (A) and / or (B) administered.
  • the premix comprises components (A), (B) and (C).
  • components (A), (B) or (C), two of components (A), (B) and (C) can be administered to the ruminating animals in any combination or all three components.
  • the components (A), (B) and / or (C) are added to the complete feed individually or in the form of a premix. If only two of the components (A), (B) and (C) are administered via the complete feed, the third component may be fed to the animals individually or in the form of a premix.
  • components (A) and (C) and individually component (B) may be administered to the animals via the complete feed, or component (B) may be used individually or in the form of a masterbatch further comprising a second portion of component (A) and / or (B) administered. If only one of the components (A), (B) and (C) is administered to the animals via the complete feed, the second and third components may be fed to the animals individually and / or in the form of a premix.
  • the components (A), (B) and (C) are administered individually to the ruminating animals.
  • components (A), (B) and (C) are in the form of a premix, preferably in the form of a premixed composition comprising at least two of components (A), (B) and (C), preferably Components (A), (B) and (C) are administered, preferably by adding them to the complete feed of the animals.
  • components (A), (B) and (C) are administered to the animals at least once a week, more preferably at least every three days, and more preferably at least once a day, whether individually or in the form of a premix or over the complete feed he follows.
  • the dosage administered to animals within 24 hours per animal is 0.01 g to 1000 g, preferably 0.05 g to 500 g, more preferably 0.1 g to 250 g, most preferably 0.25 g to 150 g and in particular 0.5 g to 50 g, based on the total amount of components (A), (B) and (C).
  • the dosage of component (A) administered to the animals within 24 hours is from 0.001 g to 500 g, preferably from 0.01 g to 100 g, more preferably from 0.1 g to 10 g, per animal.
  • the dosage of component (B) administered to the animals within 24 hours is from 0.001 g to 500 g, preferably from 0.01 g to 100 g, more preferably from 0.1 g to 20 g, per animal.
  • the dosage of component (C) administered to the animals within 24 hours is from 0.005 g to 750 g, preferably from 0.05 g to 200 g, more preferably from 0.3 g to 45 g, per animal.
  • the total amount of the components (A), (B) and (C) administered within 24 hours contains the component (A) in an amount of 0.1 to 50% by weight, preferably 1 to 40% by weight. particularly preferably 2 to 30% by weight; the component (B) in an amount of 0.1 to 75 wt .-%, preferably 1 to 60 wt .-%, particularly preferably 2 to 45 wt .-%, and the component (C) in an amount of 5 to 98 Wt .-%, preferably 20 to 95 wt.%, Particularly preferably 40 to 90 wt .-%.
  • the total amount of components (A), (B) and (C) administered within 24 hours contains the component (A) in an amount of 0.1 to 50 wt .-%, preferably 1 to 40 wt .-%, particularly preferably 2 to 30 wt .-%; the component (B) in an amount of 0.1 to 85 wt .-%, preferably 1 to 80 wt .-%, particularly preferably 2 to 75 wt .-%, and the component (C) in an amount of 5 to 98 Wt .-%, preferably 10 to 95 wt.%, Particularly preferably 15 to 90 wt .-%.
  • components (A), (B) and (C) are administered to the animals in the form of a premixed composition.
  • a further subject of the present invention is therefore a composition comprising the combination comprising the components (A), (B) and (C):
  • (C) one or more substances selected from the group consisting of the (CrC 8 ) monocarboxylic acids, (C 2 -C 8 ) dicarboxylic acids and (C 3 -C 8 ) tricarboxylic acids, and their sodium, potassium , Calcium and magnesium salts.
  • the composition is an animal feed additive composition.
  • the composition Preferably in the composition at least 0.1 wt .-%, preferably 0.1 to 75 wt .-%, particularly preferably 0.1 to 50 wt .-%, most preferably 1 to 40 wt .-%; and in particular 2 to 30 wt .-%, saponin-containing plant extracts and / or plant components, based on the total weight of the composition, as component (A).
  • composition Preferably in the composition at least 0.1 wt .-%, preferably 0.1 to 75 wt .-%, particularly preferably 1 to 60 wt .-%; and in particular 2 to 45 wt .-%, antimicrobial and / or flavonoid-containing plant extracts and / or plant components, based on the total weight of the composition, as component (B).
  • composition at least 0.1 wt .-%, preferably 0.1 to 85 wt .-%, particularly preferably 1 to 80 wt .-%; and in particular from 2 to 75% by weight, of antimicrobial and / or flavonoid-containing plant extracts and / or plant constituents, based on the total weight of the composition, as component (B).
  • the composition Preferably in the composition at least 1 wt .-%, preferably at least 5 wt .-%, particularly preferably 5 to 98 wt .-%, most preferably 20 to 95 wt .-%; and in particular from 40 to 90% by weight of substances selected from the group consisting of the (CrC 8 ) -monocarboxylic acids, (C 2 -C 8 ) -dicarboxylic acids and (C 3 -C 8 ) -tricarboxylic acids, and their sodium, Potassium, calcium and magnesium salts, based on the total weight of the composition, as component (C).
  • composition at least 1 wt .-%, preferably at least 5 wt .-%, particularly preferably 5 to 98 wt .-%, most preferably 10 to 95 wt .-%; tricarboxylic acids, and their sodium, - and, in particular substances selected from the group consisting of (CrC 8) monocarboxylic acids, (C 2 -C 8) dicarboxylic acids, and (C 8 C 3) 15 to 90 wt .-%, Potassium, calcium and magnesium salts, based on the total weight of the composition, as component (C).
  • component (A) is present in an amount of from 0.1 to 50% by weight, preferably from 1 to 40% by weight, more preferably from 2 to 30% by weight; the component (B) in an amount of 0.1 to 75 wt .-%, preferably 1 to 60% by weight, particularly preferably 2 to 45 wt .-%, and the component (C) in an amount of 5 to 98 wt .-%, preferably 20 to 95 wt .-%, particularly preferably 40 to 90 wt .-%, each based on the total weight of the composition, before.
  • the composition may further contain a flow aid.
  • the flow aid is selected from the group consisting of E 551, E 561 and gum arabic.
  • the components (A) and (B) are in a relative weight ratio of 100: 1 to 1: 100, preferably from 50: 1 to 1:50, more preferably from 20: 1 to 1: 20, very particularly preferably from 10: 1 to 1:15, and in particular from 5: 1 to 1:15, in each case based on the total weight of all saponin-containing components contained in the composition as component (A), in particular plant extracts and / or plant components and the total weight all in the composition as component (B) contained antimicrobial and / or flavonoid-containing components, in particular plant extracts and / or plant components, in the inventive composition.
  • the components (A) and (C) are in a relative weight ratio of 100: 1 to 1: 100, preferably from 20: 1 to 1: 50, more preferably from 5: 1 to 1: 50, very particularly preferably from 1: 1 to 1: 30, and in particular from 1: 2 to 1: 25, in each case based on the total weight of all contained in the composition as component (A) saponin-containing components, in particular plant extracts and / or plant components and the total weight all the substances in the composition as component (C) contained is selected from the group consisting of (CrC 8) monocarboxylic acids, (C 2 -C 8) dicarboxylic acids, and (C 3 - C 8) tricarboxylic acids, and their sodium, Potassium, calcium and magnesium salts, in the inventive composition.
  • the components (B) and (C) are in a relative weight ratio of 100: 1 to 1: 100, preferably from 20: 1 to 1:50, more preferably from 5: 1 to 1: 50, very particularly preferably from 1: 1 to 1: 30, and in particular from 1: 2 to 1: 25, in each case based on the total weight of all contained in the composition as component (B) antimicrobial and / or flavonoid-containing components, in particular plant extracts and / or plant constituents and the total weight of all in the composition as component (C) contained substances selected from the group consisting of (CrC 8) monocarboxylic acids, (C 2 -C 8) dicarboxylic acids, and (C 3 - C 8) tricarboxylic acids, and their sodium, potassium, calcium and magnesium salts, in the composition according to the invention.
  • the amounts given in percentages by weight (% by weight) each refer to the total amount of components (A), (B) and (C).
  • the composition contains 1-10% by weight of sweetness as component (A).
  • the composition contains 5-15 wt .-% Yucca Shidigera, optionally 1-10 wt .-% ginseng and optionally 1-10 wt .-% Quillaya saponaria as component (A).
  • the composition contains 5-15% by weight of Yucca Shidigera, 1-10% by weight of sweet wood and optionally 5-15% by weight of clover seed as component (A).
  • the composition contains 10-30 wt .-% hop grape and / or hops extract, 5-15 wt .-% grape pomace and / or grape seed extract, optionally 1-8 wt .-% garlic and optionally 1-5 wt .-% rosemary extract as component (B).
  • the composition contains 1-15% by weight of hop manure and / or hop extract, optionally 1-5% by weight of garlic and optionally 1-5% by weight of rosemary extract as component (B).
  • the composition contains 20-40% by weight of a propionic acid salt, e.g. Caicium propionate and 20-30% by weight of fumaric acid as component (C).
  • a propionic acid salt e.g. Caicium propionate
  • fumaric acid as component (C).
  • the composition contains 5-15% by weight of an acetic acid salt, e.g. Sodium acetate, 20-30% by weight of a fumaric acid salt, e.g. Magnesium fumarate and 40-60% by weight of malic acid as component (C).
  • an acetic acid salt e.g. Sodium acetate
  • a fumaric acid salt e.g. Magnesium fumarate
  • malic acid e.g. Magnesium fumarate and 40-60% by weight of malic acid as component (C).
  • the composition contains 70-85% by weight of a fumaric acid salt, eg magnesium fumarate, and 1-10% by weight of an acetic acid salt, eg sodium acetate, as component (C). In yet another preferred embodiment, the composition contains 20-40% by weight of a fumaric acid salt, eg, magnesium fumarate, and 15-30% by weight of an acetic acid salt, eg, sodium acetate, as component (C).
  • the composition contains 15-30% by weight of hop manure and / or hop extract, 5-20% by weight of grape pomace and / or grape seed extract, 1 to 10% by weight of sweetness, 1 to 10% by weight.
  • Garlic 0.5-5% by weight of rosemary extract, 10-45% by weight of a propionic acid salt, eg Calcium propionate, and 15-40% by weight of fumaric acid.
  • the composition contains 30-60% by weight of malic acid, 20-30% by weight of a fumaric acid salt, e.g. Magnesium fumarate, 5-20% by weight of an acetic acid salt, e.g. Sodium acetate, 5-15% by weight of Yucca shidigera, and 1-10% by weight of hop sprigs and / or hop extract.
  • a fumaric acid salt e.g. Magnesium fumarate
  • an acetic acid salt e.g. Sodium acetate, 5-15% by weight of Yucca shidigera
  • hop sprigs and / or hop extract e.g., a hop extract.
  • the composition contains 60-85% by weight of a fumaric acid salt, e.g. Magnesium fumarate, 5-15% by weight Yucca Shidigera, 1-15% by weight of an acetic acid salt, e.g. Sodium acetate and 1-10% by weight of hop manure and / or hop extract.
  • a fumaric acid salt e.g. Magnesium fumarate
  • Yucca Shidigera e.g. Sodium acetate
  • an acetic acid salt e.g. Sodium acetate and 1-10% by weight of hop manure and / or hop extract.
  • the composition contains 15-50% by weight of a fumaric acid salt, e.g. Magnesium fumarate, 5-15% by weight Yucca Shidigera, 10-30% by weight of an acetic acid salt, e.g. Sodium acetate, 5-20% by weight of hop manure and / or hop extract, 1-10% by weight of ginseng, 1-10% by weight of Quillaya saponaria and 1-10% by weight of rosemary extract.
  • a fumaric acid salt e.g. Magnesium fumarate, 5-15% by weight Yucca Shidigera
  • an acetic acid salt e.g. Sodium acetate
  • hop manure and / or hop extract e.g. Sodium acetate
  • 1-10% by weight of ginseng e.g. a acetate
  • ginseng e.g. a acetate
  • Quillaya saponaria e.g., Quillaya saponaria and 1-10% by weight of rosemary extract
  • the composition contains 10-30% by weight of a fumaric acid salt, e.g. Magnesium fumarate, 5-15% by weight of Yucca shidigera, 10-60% by weight of grape pomace and / or grape seed extract, 1% -20% by weight of clover seed, 1% -10% by weight of garlic and 1% -10% by weight.
  • a fumaric acid salt e.g. Magnesium fumarate, 5-15% by weight of Yucca shidigera, 10-60% by weight of grape pomace and / or grape seed extract, 1% -20% by weight of clover seed, 1% -10% by weight of garlic and 1% -10% by weight.
  • a fumaric acid salt e.g. Magnesium fumarate, 5-15% by weight of Yucca shidigera, 10-60% by weight of grape pomace and / or grape seed extract, 1% -20% by weight of clover seed, 1% -10% by weight of garlic and 1%
  • composition contains
  • component (A) selected from the group consisting of sweetener, yucca shidigera, ginseng, quillaya saponaria, clover seeds or a mixture of two or more of these components
  • component (B) selected from the group consisting of hops, hop distillers, hops extract, grape pomace, grape seeds, garlic, rosemary and rosemary extract or a mixture of two or more of these components
  • component (C) selected from the group consisting of calcium propionate, fumaric acid, malic acid, magnesium fumarate and sodium acetate or a mixture of two or more of these components, the amounts given being based on the total weight of the components (A ), (B) and (C).
  • compositions according to the invention preferably each contain no meat, in particular no meat from chickens or chicken.
  • compositions according to the invention and the combinations used according to the invention contain no plant material, no extract and no derivative of the species Origanum.
  • the saponin content in the composition is at least 0.1 wt .-%, preferably at least 0.25 wt .-%, particularly preferably at least 0.5 wt .-%, and in particular at least 1 wt .-%, each based on the total weight of components (A), (B) and (C).
  • the flavonoid content in the composition is preferably at least 0.1% by weight, preferably at least 0.25% by weight, particularly preferably at least 0.5% by weight, and in particular at least 1% by weight, in each case based on the total weight of components (A), (B) and (C).
  • the preparation of the composition according to the invention is preferably carried out by mixing the individual components.
  • the preparation of the composition according to the invention can also be carried out by dissolving and / or suspending the individual components in a solvent and then removing the solvent.
  • Another object of the present invention is a use of the composition for reducing the methane emissions of ruminant animals.
  • composition may be mixed with the animal feed or orally administered separately from the animal feed.
  • the composition is added to the animal feed, preferably mixed homogeneously with the animal feed.
  • the amount in which the composition is added to the animal feed depends on the type and weight of the animal.
  • the composition is added to the animal feed in an amount of 0.001 g to 10 g per kg of animal feed.
  • the composition is added to the animal feed in an amount such that the content of component (A) is 0.001 g to 5 g per kg of animal feed, preferably 0.01 g to 1 g per kg of animal feed, and more preferably 0.05 g to 0.5 g per kg of animal feed.
  • the composition is added to the animal feed in an amount such that the content of component (B) is 0.001 g to 5 g per kg of animal feed, preferably 0.01 g to 1 g per kg of animal feed, and more preferably 0.05 g to 0.5 g per kg of animal feed.
  • the composition is added to the animal feed in an amount such that the content of component (C) is 0.01 g to 8 g per kg of animal feed, preferably 0.05 g to 5 g per kg of animal feed , and more preferably 0.1 g to 1 g per kg of animal feed.
  • the combination or composition is used to reduce methane emissions from ruminating animals.
  • the ruminant animals preferably belong to the family of the bovids (Bovidae) or camels (Camelidae).
  • the ruminating animals are selected from the group consisting of the goats (Caprä); Sheep (Ovis); Cattle (Bos); including water buffalo (Bubalus bubalis), tamarac (Bubalus mindorensis), lowland anoas (Bubalus depressicornis), mountain anoas (Bubalus quarlesi) African buffalo (Syncerus caffer), aurochs (Bos taurus), domestic bovines (B. t.
  • the combination or composition is used in the rearing, fattening or lactation of ruminating animals.
  • the reduction of methane emissions from ruminant animals can be determined by in vitro studies using the RUSITEC (rumen simulation technique) method. Further details of this process can be found in the examples.
  • the methane emissions during ruminal fermentation in the artificial rumen are preferably reduced by at least 3%, preferably at least 5%, and in particular at least 7.5%, 8 hours after the use of the combination or composition according to the invention.
  • compositions according to the invention some typical compositions of an animal feed additive mixture are listed below by way of example, with the statements being made in each case in percent by weight, based on the total weight of the composition.
  • the RUSITEC system is a semi-continuous in vitro incubation technique, as used herein in J.W. Czerkawski, G. Breckenridge, Br. J. Nutr. 38 (1977): 371-384 and J.W. Czerkawski (1986) "An introduction to space studies", Pergamon Press, Oxford, UK, 236 et seq.
  • the tested dosage corresponds to a dosage of 4 g of the formulation according to the invention per animal per day.
  • component C was able to reduce the methane formation on average by 2.3-4.8% in in-vitro experiments with different dosages and substrates (MD Carro, MJ Ranilla, Br. J. Nutr. 90: 617-623).

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

L'invention concerne l'utilisation d'une combinaison comprenant un ou plusieurs extraits végétaux et/ou composants végétaux à base de saponine (composant A), un ou plusieurs extraits végétaux et/ou composants végétaux antimicrobiens à base de flavonoïde (composant B) et un ou plusieurs substances sélectionnées dans le groupe comprenant les acides monocarboxyliques-(C1-C8), les acides dicarboxyliques-(C2-C8) et les acides tricarboxyliques-(C3-C8) ainsi que leurs sels de sodium, de potassium, de calcium et de magnésium (C) pour réduire les émissions de méthane d'animaux ruminants.
PCT/EP2013/065338 2012-07-20 2013-07-19 Combinaison pour réduire les émissions de méthane dans le fourrage de ruminants WO2014013068A1 (fr)

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EP12005315.2 2012-07-20
EP12005315 2012-07-20

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CN106666127A (zh) * 2016-12-27 2017-05-17 梁磊 一种海红果渣羊饲料及其制备方法
CN110944517A (zh) * 2017-06-01 2020-03-31 莫特迩公司 动物饲料补充剂
CN113367236A (zh) * 2021-06-17 2021-09-10 中国农业科学院北京畜牧兽医研究所 调控反刍动物瘤胃微生物发酵的植物源天然化合物及其应用
CN116035125A (zh) * 2023-03-07 2023-05-02 吉林省农业科学院 一种牛羊用瘤胃缓释甲烷抑制剂及其生产方法

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CN106666127A (zh) * 2016-12-27 2017-05-17 梁磊 一种海红果渣羊饲料及其制备方法
CN110944517A (zh) * 2017-06-01 2020-03-31 莫特迩公司 动物饲料补充剂
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CN110944517B (zh) * 2017-06-01 2024-06-04 莫特迩公司 动物饲料补充剂
CN113367236A (zh) * 2021-06-17 2021-09-10 中国农业科学院北京畜牧兽医研究所 调控反刍动物瘤胃微生物发酵的植物源天然化合物及其应用
CN116035125A (zh) * 2023-03-07 2023-05-02 吉林省农业科学院 一种牛羊用瘤胃缓释甲烷抑制剂及其生产方法

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