Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
  • A23L27/60—Salad dressings; Mayonnaise; Ketchup
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L23/00—Soups; Sauces; Preparation or treatment thereof
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L23/00—Soups; Sauces; Preparation or treatment thereof
  • A23L23/10—Soup concentrates, e.g. powders or cakes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
  • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
  • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
  • A23L29/231—Pectin; Derivatives thereof
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Y—ENZYMES
  • C12Y301/00—Hydrolases acting on ester bonds (3.1)
  • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
  • C12Y301/01011—Pectinesterase (3.1.1.11)
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

• the present invention relates to a concentrate food composition comprising water, salt and pectin. It further relates to a process to prepare the same. It further relates to the use of said composition for preparing a bouillon, a soup, a sauce, a gravy or a seasoned dish.
• High-salt concentrated food compositions which after dilution in water or a dish result in a bouillon, a soup, a sauce or a gravy or a seasoned dish have been described.
• This class of products is known to the consumer as well as to the skilled person for many decades. They are known for example in the form of bouillon cubes, soup cubes or seasoning cubes.
• Concentrate products can be dry (e.g. dehydrated), liquid, or paste-like products which, after the addition to water according to the directions for use, yield ready to eat preparations. Recently, savoury concentrate food compositions became
• these gelled concentrates can contain more water and are considered to have a more fresh appearance, while still having a high-salt level, e.g. to allow high dilution ratios while maintaining a desired taste impact.
• a high-salt level e.g. to allow high dilution ratios while maintaining a desired taste impact.
• the gelled concentrates show the advantage that they are sufficiently firm (also sometimes referred to in the art as self-sustaining, shape-stable, soft-solid or semi- solid) , and non-sticky and therefore can be unit dosed, like a traditional bouillon cube.
• Gelled concentrates rely for their firm, semi-solid, gelled texture on the presence of a gelling agent.
• a gelling agent can comprise one or more gelling and/or thickening agents which together provide the semi-solid firm gel texture.
• the gelling agent should be compatible with high-salt levels.
• WO2007/068484 describes a savoury gelled concentrate comprising a gelling agent which contains a mixture of xanthan gum and locust bean gum.
• EP2468110 describes a food composition
• this diluted composition shows an undesired considerable increase in
• the aim of the present invention is to provide a concentrate food composition for example to provide a bouillon, soup, sauce, gravy or to season a meal, which concentrate food composition is in the form of a gel at room temperature and which is shape-stable at high-salt levels, in this way allowing the conventional dilution ratios as used for concentrate food compositions like bouillon cubes, soup cubes, or seasoning products, (e.g. 5 to 50 times by weight) .
• the aim of the invention is to provide these concentrate food compositions wherein the increase in viscosity upon cooling of the diluted concentrate food composition is very low, preferably is negligible. Further it is desired that the spoonability of the concentrate food composition is relatively easy and natural.
• a further aim of the present invention is to provide a process to produce a food composition of the invention which is stable at high-salt content and which is in the form of a semi-solid gel at room temperature.
• the amount of gelling agent present in the resulting concentrate food composition is relatively low and the amounts of gelling agent added to the ingredients mixture during preparation are consequently
• composition in the form of a gel comprising:
• pectin ⁇ from 0.3 to 10 wt% of pectin based on the total water content of the food composition, pectin being all pectin with a degree of esterification of lower than 55%, wherein the amount of said pectin is measured as the amount of galacturonic acid (GalA) , and is
• free pectin comminuted plant material
• said free pectin and said bound pectin are present in a ratio of from 0.2 to 200.
• the present invention relates to a process to prepare a concentrate food composition according to the invention, the process comprising the steps of:
• process further comprises at least one of the following steps:
• the present invention relates to the use of a food composition of the present invention to prepare a bouillon, a soup, a sauce, a gravy or a seasoned dish. Detailed description of the invention
• the food composition of the present invention is in the form of a semi-solid gel. It is not a paste. Preferably the gel is a self-sustaining gel.
• a semi-solid gel is known to the person skilled in the art of gelled concentrate food compositions. A semi-solid gel texture allows the consumer of the food
• Such a semi-solid gel may allow making easy scoops for example with a spoon, which may be preferred for multi-dosage packagings .
• the semi-solid, preferably self-sustaining gel texture is present at least at room temperature (20°C) .
• the semi-solid gel texture prevents that the food composition flows apart during or after removal from its packaging, like a liquid or a paste, and allows it to maintain the shape, which at least to a certain extent reflects the shape the product had when present in its packaging.
• the product is preferably not sticky, such as a paste (for example tomato paste) .
• the gel is
• the texture of the semi-solid gel can for example be analysed by a texture analyser, as known in the art.
• the texture can be characterised for example using common techniques such as texture analysis of penetration and compression, as measured in a penetration test, with equipments such as a Texture Analyser (e.g. from Stable MicrosystemsTM) or a Universal testing machine (e.g. from InstronTM) .
• a Texture Analyser e.g. from Stable MicrosystemsTM
• a Universal testing machine e.g. from InstronTM
• a plunger In a "penetration test", a plunger is forced into a composition and the force required for penetration of the composition is plotted against the distance (or time) of penetration into the composition at a pre-determined speed to a pre-determined depth of penetration. The plunger is then withdrawn.
• two consecutive penetrations was used. If a composition is in the form of a (brittle) semi ⁇ solid gel (as preferred in this invention) it typically shows a breaking point (or irreversible deformation, e.g. yield) in the first penetration, and the maximum force is reached, indicating the "product firmness". If a composition is in the form of a paste or a very elastic gel, the product firmness (maximum force) is commonly observed at the maximum distance (depth) of penetration.
• the area under the force versus distance curve of the first penetration defines the area in the graph Al of
• Test type Penetration test with 2 cycles a. The_measurements are performed after at least 12h
• maturation time after the samples are prepared and gelled (solidified) is preferred.
• a longer maturation time of for example 24h to 48h is preferred.
• the samples are equilibrated to room temperature for at least 2h, prior to measurement.
• Sample height at least 25 millimeters
• Penetration depth 10 millimeters (measurement error can be typically of 0.1-0.2 mm) .
• the composition of the invention is not liquid, but has a semi-solid texture with certain firmness.
• the firmness is determined as the maximum force (or breaking point) in the penetration first cycle (expressed in g) .
• the maximum force (firmness) is typically observed as a breaking point much before complete penetration depth (distance is less than the penetration dept, which is 10mm) .
• the penetration dept which is 10mm
• firmness (in g) is preferably higher 15 g, more preferably higher than 20 g, even more preferably higher than 30 g, most preferably higher than 40 g.
• the firmness is preferably less than 1000 g, more preferably less than 700 g, even more
• Gels of the invention are preferably brittle gels. It is preferred that the gels have a certain brittleness so they are easier to spoon and easy to disperse in the
• Brittleness is defined, for the purpose of this invention, as the distance of penetration until the maximum force is achieved (in milimeter) in the first penetration.
• a semi-solid gel as in the present invention i.e. brittle gels
• brittle gels that is typically observed at a breaking point, at a distance (in milimeters) less than the penetration depth defined (10 mm) .
• Figure 2a Contrary to a semi-solid gel, a paste may be too sticky and is not brittle, it does not break. This is illustrated in Figure 2b.
• An elastic gel might also not break within the penetration depth imposed in the test (10mm) . This is illustrated in Figure 2c.
• the gel of the invention is preferably not an elastic gel.
• the brittleness is preferably less than 9 millimeter, more preferably less than 8 millimeter, even more preferably less than 7 millimeter.
• the recovery of the composition is expressed as the ratio A2/A1:
• the ratio between A2/A1 is considered as a measure for the cohesiveness of the composition, i.e. is a measure of how well the product withstands a second deformation relative to how it behaved under the first deformation.
• the recovery is preferably less than 80%, more preferably less than 70 ⁇ 6 , even more preferably less than 60%.
• semi-solid (brittle) gels as in this invention show a lower recovery value than very elastic gels, liquids, purees and pastes, as they break in the first cycle (i.e. breaking point is observed in the first cycle at a distance below 10 mm) .
• the food composition according to the invention comprises water.
• Water is preferably present in a total amount of from 35 wt% to 93 wt%. More preferably water is present in an amount of from 40 wt% to 85 wt%, even more preferably from 45 wt% to 80 %wt, most preferably from from 50 wt% to 75 %wt .
• Water is representing here the total water content of the food
• composition .
• the water activity of the product is preferably of between 0.60 and 0.95, more preferably of between 0.65 and 0.90 even more preferably between 0.70 and 0.90, most preferably between 0.75 and 0.85.
• the concentrate food composition according to the invention comprises comminuted plant material.
• the term "comminuted plant material" in the composition of the invention preferably refers to material originating from vegetables or fruits. During the preparation of the composition of the invention, coarse plant material is comminuted, for example by shearing or milling.
• the comminuted plant material normally contains water-insoluble components made of polymers such as cellulose, hemicelluloses and lignin.
• a vegetable or fruit juice (or serum) although derived from plant material is not considered a comminuted plant material in the context of the present
• the comminuted plant material in the composition of the invention therefore preferably is plant material selected from the group consisting of plant cells, plant cell fragments, clusters of plant cells and/or plant cell fragments, and mixtures thereof.
• This comminuted plant material can be
• the size of the comminuted plant material is suitably in the range of the size of cell fragments to cell clusters.
• the comminuted plant material has a size of between 10 micron and 1500 micron, preferably of between 20 micron and 1200 micron, more
• the comminuted plant material has a size of between 10 micron and 1500 micron, preferably of between 20 micron and 1200 micron, more preferably of between 30 micron and 1000 micron, most preferably of between 50 micron and 800 micron.
• the size should be understood as defined by mesh size, so that the comminuted plant material can be sieved using a sieve with a mesh size as indicated for the (preferred) sizes of the comminuted plant material.
• the food composition according to the invention preferably comprises the comminuted plant material, preferably selected from the group consisting of plant cells, plant cell fragments, clusters of plant cells and/or plant cell fragments, and mixtures thereof in an amount of from 10 to 91 wt ⁇ 6 , more preferably from 20 to 90 wt%, even more preferably from 30 to 89 wt%, even more preferably from 35 to 85 wt ⁇ 6 , even more preferably of from 35 wt% to 80 wt%, most preferably from 40 to 70 wt% (wet weight based on the weight of the total concentrate food composition) .
• the comminuted plant material is preferably selected from the group consisting of onion, carrot, broccoli, courgette,
• cauliflower pea, pumpkin, leek, celeriac, shallot, garlic, potato, sweet potato, green beans, tomato and mixtures thereof.
• the comminuted plant material is preferably selected from the group consisting of onion, carrot, broccoli, courgette,
• the amount of comminuted material derived from tomato is less than 50 wt%, more preferably less than 30 wt%, even more preferably less than 10 wt% or even less than 1 wt%, based on the weight of the food concentrate.
• the comminuted plant material is preferably selected from the group consisting of onion, carrot, broccoli, courgette, cauliflower, pea, pumpkin, leek, celeriac, shallot, garlic, potato, sweet potato, green beans and mixtures thereof. Particular good results were obtained when the comminuted plant material was selected from the group consisting of members of the Allium family, like onion, shallot, leek, garlic and mixtures thereof. In particular onion is preferred and gave good results in the present invention.
• Pectic substances are complex heteropolymers originating from plant tissue.
• Pectin consists predominantly of a-D galacturonic acid units, but also contains some amount of neutral sugars such as rhamnose, xylose, arabinose, galactose and gulose.
• pectin is expressed as
• galacturonic acid and we define the “pectin content” in a food formulation as the weight percentage of galacturonic acid (GalA) based on the total water content of the composition.
• the total pectin content can be determined by methods known in the art, such as for example the Saeman hydrolysis method (Englyst and Cummings (Analyst, 109(7), 937-942 (1984), Filisetti-Cozzi and Carpita (Analytical Biochemistry, 197, 157-162 (1991)).
• the carboxyl groups at the sixth carbon of each galacturonic acid unit may be esterified by a methyl group or may exist as unesterified free carboxyl group. The percentage of esterified galacturonic acid units relative to the total number of
• the degree of esterification can be determined according to methods known in the art, such as the base titration method (Shultz, 1965) as proposed by the Food Chemical Codex (FCC (1981). 3rd ed., (1981) National
• NMR nuclear magnetic resonance
• CZE capillary zone electrophoresis
• the DE resulting from such a determination is usually expressed as an average degree of esterification to account for the differences in DE of the individual polymers in a formulation.
• the average degree of esterification (DE) is often used to classify pectin according to physical characteristics like the ability to form gels in the presence of divalent cations like calcium.
• Complex food formulations of the present invention may contain pectin from different sources with a variety of
• total active pectin TAP
• TIP total inactive pectin
• TAP can be defined as being all pectin of the "total pectin content” with a DE below 55%.
• DE is below 50% even more preferably below 45%, most preferably the DE is below 40%.
• a method to separate pectin into fractions with different DE is described, for example, by , Strom et al . (2005), Carbohydrate Polymers, Volume 60, Issue 4, 20 June 2005, Pages 467-473.
• the TAP is quantified by the sum of all fractions of the "total pectin content” with a DE below 55%.
• the amount of total active pectin is between 0.3% and 10%, preferably between 0.4 wt% and 5 wt%, more preferably between 0.45 and 4.5 wt ⁇ 6 even more preferably between 0.5 wt% and 3.5 wt%, most preferably between 0.6 wt% and 3.0 wt%, expressed as the weight percentage of galacturonic acid (GalA) based on the total water content of the food composition.
• GalA galacturonic acid
• This is to be calculated as common in the art, i.e. according to the following formula: ((weight of galacturonic acid) / (weight of galacturonic acid + weight of total water content)) x 100 in %.
• This formula is used, mutatis mutandis for all ingredients in the composition which are calculated on the total water content, such as AFP, NaCl, calcium ions, potassium salt.
• the concentrate food composition of the present invention comprises from 0.3 to 10 wt% of pectin based on the total water content of the food composition, pectin being all pectin with a degree of esterification of lower than 55%, wherein the amount of said pectin is measured as the amount of galacturonic acid (GalA) , and is calculated as ( (weight of GalA)/ (weight of GalA + weight of total water content) ) xlOO in %,
• the DE is lower than 50% even more preferably lower than 40%, most preferably the DE is lower than 30%.
• total active pectin (TAP) is preferably of between 0.5 wt% and 10 wt%, more preferably of between 0.6 wt% and 5 wt ⁇ 6 , even more
• total active pectin (TAP) is preferably of between 0.3 wt% and 5 wt preferably of between 0.4 wt% and 4 wt%, even more preferably between 0.5 wt% and 3 wt%, most preferably between 0.6 wt% and 2 wt%, expressed as galacturonic acid content based on the total water content of the food composition.
• pectin is present both in the form of free pectin and in the form of bound to comminuted plant material.
• Part of the total pectin content i.e. TAP and TIP together, is bound to the comminuted plant material, as defined above.
• the part of the total pectin content bound to comminuted plant material is denoted as "bound pectin”.
• active bound pectin (ABP)_ is distinguished from inactive bound pectin, based on the degree of esterification of the pectin.
• the other part of the total pectin content i.e.
• free pectin active free pectin (AFP) is distinguished from_inactive free pectin based on the degree of esterification of the pectin.
• ABSP active pectin bound to comminuted plant material
• the amount of active free pectin (AFP) is preferably of from 0.15 wt% to 8 wt%, more preferably of from 0.20 wt% to 5wt%, even more preferably of from 0.25 wt% to 4 wt%, most preferably of from 0.30 to 3 wt% expressed as the weight percentage of galacturonic acid (GalA) based on the total water content of the food composition.
• GalA galacturonic acid
• AFP/ABP can be calculated according to the formula [ (wt% active free pectin) / (wt% active bound pectin] .
• AFP and ABP are expressed as GalA.
• the ratio of active free pectin to active bound pectin AFP/ABP is between 0.2 and 200, preferably between 0.3 and 100, even more
• the present invention relates to a concentrate food composition wherein part of the pectin is bound to comminuted plant material, and part of the pectin is not bound (free) to comminuted plant material and said free and said bound pectin are present in a ratio of from 0.2 to 200.
• the ratio is between 0.3 and 100, even more preferably between 0.4 and 40, even more preferably between 0.5 and 20, most preferably between 0.7 and 10.
• the food composition according to the present invention is a concentrate product that can be used to prepare for example a bouillon, a soup or a seasoned dish.
• a product is diluted, for example in water, or in a dish, for example in a liquid dish or a in sauce or in a vegetable or rice dish, to obtain a food product which is ready for consumption.
• the concentrate food composition according to the invention comprises a high salt content, to allow the
• the food composition according to the invention preferably comprises of from 5 wt% to 40 wt% of sodium salt and optionally potassium salt (taken together) , more preferably of from 7 wt% to 35 wt%, even more preferably of from 10 wt% to 35 wt ⁇ 6 , even more preferably of from 12 wt% to 30 wt%, most preferably of from 15 to 27 wt%, based on the total water content of the concentrate food composition.
• the amount of sodium salt and optionally potassium salt is calculated as standard in the art, and is according to the following formula: ((weight of salt) / (weight of salt + weight of total water content) ) *100 in %. For example 5 g NaCl in 20 g water result in an amount of NaCl of 20 wt% on water content.
• these amounts of sodium salt and optionally potassium salt can be added during preparation.
• Sodium salt preferably NaCl
• Sodium salt is preferably present in an amount of from 5 to 40%, more preferably of from 7 wt% to 35 wt%, even more preferably of from 10 wt% to 35 wt
• the concentrate food composition preferably of from 12 wt% to 30 wt%, most preferably of from 15 to 27 wt%, based on the total water content of the concentrate food composition.
• the food composition comprises a potassium salt (e.g. KC1) .
• KC1 potassium salt
• sodium salt preferably NaCl
• sodium salt is preferably present in an amount of from 4 to 35 wt%, more preferably of from 4.5 to 30 wt%, even more preferably of from 5 to 25 wt%, most preferably of from 7 to 23 wt%, based on the total water content .
• the food composition preferably comprises potassium salt.
• the potassium salt comprises KC1.
• potassium salt, preferably KC1 is preferably present in an amount of from 0.6 to 20 wt%, more preferably of from 0.8 to 19wt%, even more preferably of from 1 to 17 wt%, most
• compositions preferably of from 1.5 to 15 wt%, based on the total water content of the composition.
• the food composition of the present invention comprises Na + and K + cations in a ratio of
• potassium salt preferably KC1
• potassium salt is preferably present in an amount to result in an amount of potassium salt (preferably KC1) of from 0.6 to 20 wt%, based on the total water content and a ratio of [ (NaV (Na + +K + ) ) *100 (in%) ] of from 15 wt% to 95 wt% in the final concentrate food composition.
• the food composition preferably comprises Na + cations in an amount of from 1.5 wt% to 15 wt%, more preferably in an amount of from 1.7 wt% to 12 wt%, even more preferably in an amount of from 2 wt% to 11 wt%, most preferably from 2.5 to 10% based on the total water content of the concentrate.
• the amount of K + cations in the food composition is preferably in an amount of from 0.3 wt% to 13 wt%, more preferably in an amount of from 0.4 wt% to 10 wt%, even more preferably in an amount of from 0.5 wt% to 9 wt%, most preferably from 0.8 to 8 wt%, based on the total water content of the concentrate.
• the food composition according to the invention comprises calcium salt.
• the food composition according to the invention comprises Ca 2+ cations in an amount of from 0.01 wt% to 3 wt% Ca 2+ , more preferably from 0.02 wt% to 2 wt% Ca 2+ , even more preferably from 0.03 wt% to 1.5 wt%Ca 2+ , most preferably from 0.04 wt% to 1 wt%Ca 2+ based on the total water content of the food concentrate.
• the food composition of the present invention further might also comprise Mg 2+ in addition to Ca 2+ .
• the food composition according to the invention comprises Mg 2+ in an amount of from 0.01 wt% to 3 wt% Mg 2+ , more preferably from 0.02 wt% to 2 wt% Mg 2+ , even more preferably from 0.03 wt% to 1.5 wt% Mg 2+ , most preferably from 0.04 wt% to 1 wt% Mg 2+ based on the total water content of the concentrate.
• the food composition according to the invention comprises Ca 2+ in an amount of from 10 to 2000 mg Ca 2+ /g of pectin, more preferably from 20 to 1000 mg Ca 2+ /g of pectin, even more preferably from 30 to 800 mg Ca 2+ /g of pectin, most preferably between 40 to 300 mg Ca 2+ /g of pectin (pectin as defined above, i.e. pectin being all pectin having a DE of lower than 55%, or less if preferred) . It might be preferred that the invention comprises Ca 2+ in an amount of from 100-300 mg Ca 2+ /g of pectin.
• the concentrate food composition of the present invention may further comprise a savoury taste enhancer selected from the group consisting of monosodium glutamate (MSG), 5 ' -ribonucleotides , organic acid and mixtures thereof.
• Savoury taste enhancer is preferably present in a total amount of less than 30 wt%, more preferably of between 0.1 wt% and 30 wt%, preferably in an amount of from 1 wt% to 25 wt%, most preferably in an amount of from 5 wt% tol5 wt%, based on the weight of the total food concentrate.
• An individual taste enhancer selected from the group mentioned above may be present in an amount of less than 30 wt ⁇ 6 , more preferably of between 0.1 wt% and 30 wt%, preferably in an amount of from 1 wt% to 25 wt%, most preferably in an amount of from 5 wt% tol5 wt%, based on the weight of the total food concentrate .
• Taste imparting components may be present in an amount of less than 30 wt ⁇ 6 , more preferably of between 0.1 wt% and 30 wt%, preferably in an amount of from 1 wt% to 25 wt%, most preferably in an amount of from 5 wt% tol5 wt%, based on the weight of the total food concentrate .
• taste-imparting components are present. They may comprise one or more of yeast extract; hydrolyzed proteins of
• liquid or dissolvable extracts or concentrates selected from the group consisting of meat, fish, crustaceans, plant material (e.g. herbs, fruit, vegetable) and mixtures thereof, particles of meat; particles of fish; particles of crustaceans; particles of plant (e.g. herbs, vegetable, fruit); particles of fungi (e.g. mushroom); flavours and mixtures thereof.
• plant material e.g. herbs, fruit, vegetable
• particles of fungi e.g. mushroom
• flavours and mixtures thereof e.g. mushroom
• the plant pieces comprise pieces selected from the group consisting of onion, garlic, leek, carrot, parsley, tomato and mixtures thereof.
• the amount of taste-imparting components as set out above is from 1 wt% to 60 wt% (by weight on the total
• the amount of particles preferably particles selected from the group of particles of meat, particles of fish, particles of crustaceans, particles of plant (e.g. herbs, vegetable, fruit), particle of fungi (e.g. mushroom) and mixtures thereof is from 0.5 wt% to 60 wt%, more preferably from 1 wt% to 50 wt%, even more preferably from 2 wt% to 40 wt% (by weight on the total concentrate) .
• the amount of particles can be of from 0.5 to 30 wt%, more preferably of from 1 to 20 wt%, even more preferably of from 2 to 10 wt% (wet weight based on the weight of the food composition) .
• the size (measured as mesh size by sieve analysis) of the particles is larger than 0.5 millimetre (500 micron), more preferably larger than 1 millimetres (1000 micron), preferably larger than 1.5 mm (1500 micron), preferably larger than 2 mm (2000 micron) .
• the size is smaller than 2 cm, more preferably smaller than 1 cm.
• Preferred ranges of the size of the particulates can be from more than 0.5 mm and less than 2 cm, preferably of from more than 1 mm and less than 1 cm, even more preferably of from 1.5 mm to 1 cm.
• Fat may be present in the food concentrate according to the present invention in relatively low amounts.
• Fat can be liquid fat or solid fat, at ambient temperature, such as for example at 20°C.
• a fat is one of the fats selected from the group consisting of chicken fat, pork fat, beef fat, and mixtures thereof. It can preferably be a fat selected from the group consisting of palm oil, sunflower oil, olive oil, rape seed oil and mixtures thereof. It can be a vegetable fat or an animal fat. Higher amounts are preferably prevented as they may interfere with the proper texture of the gel or may result in phase separation during storage or transport. Relatively high amounts of hard fat, such as e.g. saturated or hydrogenated fats may affect the desired gel texture, and therefore are not preferred.
• the present invention relates to a food concentrate further comprising less than 15 wt% of fat, preferably less than 10 wt% of fat.
• fat may be present in an amount of from 0.5 to 15 wt% of fat, more preferably of from 1 to 10 wt% of fat, most preferably of from 3 to 10 wt% of fat, based on the weight of the food concentrate.
• the amount of fat in the food concentrate is preferably as low as possible, for optimal stability. It may be preferred that fat is absent.
• the concentrate food composition of the invention is a savoury food composition.
• the sugar content in the composition according to the invention is preferably lower than 50 wt%, more preferably lower than 40 wt ⁇ 6 , even more preferably lower than 30 wt%, more preferably lower than 15 wt%, most preferably lower and 10 wt%. It can be more than 1 wt%, preferably more than 5 wt% based on the total weight of the concentrate. A suitable range could be of between 1 and 20 wt%, preferably of from 3 to 15 wt% based on the total weight of the concentrate. It may be preferred that the composition is free from sugar or free from any added sugar.
• Sugar polyols could also provide a sweet taste to the product resulting after dilution. The consumer may not appreciate the presence of these compounds.
• concentration of sugar polyols for example liquid sugar polyols, is preferably less than 1 wt ⁇ 6 , more preferably less than 0.5 wt%, even more preferably less than 0.1 wt%, or less than 0.05 wt% based on the weight of the food concentrate. Most preferably the composition does not contain any added sugar polyol or added liquid sugar polyol.
• the pH (measured at room temperature, e.g. 20 °C) of the concentrate food composition of the invention is preferably higher than 3.5, preferably between 3.5 and than 7, more preferably higher than 3.8, more preferably between 3.8 and 6, even more preferably higher than 4.0, even more preferably between 4.0 and 5.5. These levels provide a good texture of the gel and optimal taste in the product resulting after dispersing in water or a dish.
• Packaging/size :
• the food composition according to the invention is preferably packaged. It is preferably packaged in a tub, wherein the tub preferably comprises one food composition.
• the weight of the food composition is preferably of betweenlO gram to 500 grams, more preferably of between 15 grams and 300 grams. It may be preferred that the weight of the food
• composition is between 10 and 200 grams, more preferably of between 15 and 100 grams. This format is in particular useful for unit dosing for family portions. It may be preferred that the food composition has a weight of between 50 grams and 500 grams, preferably of between 100 grams and 350 grams. This packaging may be suitable for restaurant applications.
• the present invention further relates to a process to produce a food composition of the invention.
• the process comprises the steps of:
• Step a) Providing comminuted plant material which has been heated
• the process comprises a first step wherein comminuted plant material is provided.
• the plant material is preferably selected from the group of plants onion, carrot, broccoli, courgette, cauliflower, pea, pumpkin, leek, celeriac, shallot, garlic, potato, sweet potato, green beans, tomato and mixtures thereof.
• the plant material is preferably selected from the group of plants onion, carrot, broccoli, courgette, cauliflower, pea, pumpkin, leek, celeriac, shallot, garlic, potato, sweet potato, green beans, and mixtures thereof.
• Particular good results were obtained when the plant material was selected from the group consisting of members of the Allium family, like garlic, onion, shallot, leek and mixtures thereof, in particular onion.
• step a) may further comprise cutting and/or peeling of plant material.
• the plant material is fresh and preferably is not frozen plant material or defrosted plant material, i.e. which has been frozen before. The use of frozen of defrosted material resulted in less optimal results.
• Comminuting is preferably carried out after heating of plant material but can be carried out during or even before heating if preferred.
• Comminuting preferably comprises cutting, milling, or shearing of the material. Comminution of the material is carried out to obtain a small size of the
• comminuted plant material preferably in the range of that of cellular fragments, individual cells or clusters thereof.
• more than 80 wt% of the comminuted plant material is plant material selected from the group consisting of of plant cells, plant cell fragments, clusters of plant cells and/or plant cell fragments, and mixtures thereof.
• at least 80% by weight of the comminuted plant material has a size of between 10 micron and 1500 micron, preferably of between 20 micron and 1200 micron, more preferably of between 30 micron and 1000 micron, most preferably of between 50 micron and 800 micron.
• the comminuted plant material is also heated, before or after comminuting, preferably before.
• Heating deactivates enzyme activity inside the plant material, e.g. pectinase activity, and may soften the texture of the plant material. Heating is preferably carried out at a
• heating is carried out at a temperature of higher than 50°C.
• heating is carried out at a temperature of higher then 70 °C more preferably of higher than 80°C, even more preferably higher than 85°C.
• the temperature is preferably below 150°C, more preferably below 120°C, even more preferably below 100°C, most preferably below 95°C.
• Heating may be carried out at a temperature of between 50°C and 150°C, preferably of between 70°C and 120°C, more preferably of between 80°C and 95°C. It is in the skill of the artisan to adjust the temperature and time setting to achieve the enzyme deactivation and material softening. Heating can suitably be carried out in a batch process or continuous process, for example by using heat exchangers or other
• Step b) comprises adding pectin methylesterase (PME) to the comminuted plant material and allowing to_incubate.
• PME is preferably added after providing the comminuted plant material in step a) . It might also be preferred that PME is added after addition of NaCl (step d) , or after addition of calcium salt (step e) .
• PME provides a de-esterification of the methyl groups at the pectin molecule.
• the PME preferably comprises PME selected from the group consisting of fungal PME, vegetable PME and mixtures thereof.
• the added PME enzyme preferably comprises fungal PME.
• the added PME enzyme preferably is fungal PME.
• the PME enzyme is preferably a purified PME enzyme, most preferably a purified fungal PME enzyme. Purified should be construed here as not being already present in the vegetable material of step a) , but added to the mixture during preparation of the
• composition of the present invention is a composition of the present invention.
• the activity of the PME enzyme can be determined as known in the art for example by titration of the carboxylic acid groups, which have been released from pectin substrate in time with a sodium hydroxide solution, using a pH-Stat set up.
• the substrate consists of methylated pectin, water and salt.
• the activity is expressed in Pectin Esterase Units (PEU) .
• PEU Pectin Esterase Units
• One unit (PEU) is defined as the amount of enzyme that
• esterase that is added is preferably of between 0.1 and 9000 pectin estearase units (PEU), more preferably of between 0.2 andlOOO PEU, even more preferably of between 0.2 and 300 PEU, even more preferably of between 0.3 and 200 PEU most preferably between 0.3 and 100 PEU per kg of comminuted plant material ⁇
• PEU pectin estearase units
• the required incubation time may depend on factors like the amount of enzyme that is used, the amount of comminuted plant material, the type of enzyme and the incubation temperature, e.g. by using the information from the enzyme supplier regarding a selected PME enzyme, it is within the skill of the artisan to determine the incubation time needed.
• the reaction can be followed for example by checking the pH decrease during incubation time and allow incubation e.g. till a plateau phase is reached.
• step b) if deemed necessary or preferred, for example in view of an acidification step which includes adjusting the pH of the comminuted plant material to a pH of between 1 and 3 as described below, the pH and temperature are readjusted before the addition of the pectin methyl esterase (PME) enzyme, for optimal performance of the PME enzymes .
• PME pectin methyl esterase
• the optimal pH and temperature to achieve best enzyme performance can be easily found by a person skilled in the art, and depends on the source of the enzyme that is used, e.g. fungal, or vegetable and is generally defined and provided by the ingredient supplier.
• the pH can be adjusted to a pH of between 3.5 and 7, preferably of between 3.5 and 5.
• the pH adjustment can be achieved by using a suitable food grade acid or base such as hydrochloric, acetic acid, citric acid,
• the temperature is adjusted to the optimal temperature for the PME enzyme performance.
• the temperature is preferably between 10 °C and 50 °C.
• Optimal temperatures are obtained from supplier information or can be determined by a person skilled in the art.
• step b) may further comprise the step of adjusting the temperature and/or the pH to allow optimal PME activity.
• step c) After the enzyme incubation in step b) , the PME enzyme is inactivated in inactivation step c) .
• inactivation step comprises heating of the material resulting from step b) to a temperature of higher than 60 °C. Preferably heating is carried out to a temperature of higher then 70°C more preferably of higher than 75°C, even more preferably higher than 80°C. The temperature is preferably below 150°C, more preferably below 120°C, even more preferably below 100°C, most preferably below 95°C.
• the heating step deactivates the PME enzyme.
• the heating step for the PME inactivation is preferred although not the only possibility for inactivation. Alternatively, the PME inactivation can be achieved by other methods, for example, by combining heating to lower
• PME inactivation is, for example, inactivation by pulsed electric fields or by the use of enzyme inhibitors
• the process further comprises a step d) of adding sodium salt, sodium salt is preferably added to the comminuted plant
• NaCl is preferably added in an amount of from 4 wt% to 40 wt%, more preferably of from 7 wt% to 40 wt%, even more preferably of from 10 wt% to 35 wt%, more preferably of from 12 wt% to 30 wt%, most preferably of from 15 to 27 wt%, based on the total water content of the resulting food composition.
• a potassium salt like KC1 is used, it is preferably added at the moments as preferred for sodium salt.
• the potassium salt, preferably KC1 is preferably added in an amount to result in an amount of from 0.9 to 15 wt%, more preferably of from 1 to 10 wt%, even more preferably of from 1.2 to 8 wt%, most
• the potassium salt preferably KC1
• the potassium salt is added in an amount to achieve a ratio of [ (NaV (Na + +K + ) ) *100 (in%) ] of from 15% to 95%, more preferably from 35 % to 93 %, even more preferably of from 40% to 92%, even more preferably of from 45% to 90%, most preferably from 50% to 85% in the final resulting concentrate food composition of the invention.
• the preferred amounts and ratios can be combined and preferably potassium salt,
• KC1 preferably KC1
• KC1 is added in an amount to result in an amount of from 0.6 to 20 wt% KC1 and a ratio of [ (NaV (Na + +K + ) ) *100
• step d) comprises heating after addition of the salt (e.g. sodium salt, potassium salt and/or calcium salt) .
• the salt e.g. sodium salt, potassium salt and/or calcium salt
• the salt can be present in an amount which is higher than the saturation point of the salt. In this case, salt crystals may appear e.g. at the surface of the resulting concentrate.
• the process further comprises the step e) of adding of calcium salt.
• Mg 2+ cations can be added to the mixture in the form of a magnesium salt.
• Step e) can preferably be carried out after incubation with PME (i.e. after step b) , more
• step c preferably after inactivation of PME (i.e. after step c) , most preferably during or after addition of sodium salt (i.e. during or after step d) . It could for example be added after during step f) , i.e. when the mixture is transferred to a packaging.
• Preferably calcium salt is added to the comminuted plant material to a total concentration of Ca 2+ cations in the resulting concentrate food composition according to the
• Calcium cations can be added by addition of a calcium salt, for example CaCl2 or CaS0 4 .
• the process may further comprise the step of addition of taste imparting components and/or savoury taste enhancer.
• This step may comprise adding vegetable pieces, fruit pieces, herb pieces, meat pieces, fungi pieces and mixtures thereof.
• Taste imparting components, savoury taste enhancers and fat can be added in the amounts as described above under "other
• preferred ranges of the size of the particulates can be of from more than 0.5 mm and less than 2 cm, preferably of from more than 1 mm and less than 1 cm, even more preferably of from 1.5 mm to 1 cm.
• the taste imparting components may be added in an amount of from 1 to 60 wt%, based on the weight of the total resulting food composition.
• the savoury taste enhancer may be added in an amount of from 0.1 to 30 wt%, based on the weight of the total resulting food composition.
• Fat may be added in an amount of below 15 wt%, more preferably below 10 wt%, based on the weight of the resulting concentrate food composition.
• step f) the mixture resulting from step e) is packaged and allowed to solidify.
• the product may be in liquid form and is transferred to a packaging, such as a tub.
• Solidification preferably takes place in the packaging.
• Solidification preferably comprises cooling. Solidification is preferably carried out at a temperature of between 3 and 40 °C. In addition to the steps described above, at least one of the three additional steps as described below is carried out to obtain a food concentrate in the form of a gel according to the invention : Acidification :
• the process according to the invention further comprises the step of adjusting the pH of the
• This acidification step may be done during or after step a) , but is preferably carried out after step a) . It is carried out before inactivation step c) and preferably carried out before step b) . It is preferred that this reduced pH is maintained for a period of less than 2h, more preferably of less than lh, even more preferably of less than 30 min. It is preferred that this reduced pH is maintained for a period of between 1 minute and lhour, more preferably of between 5 minutes and 30 minutes.
• This step resulted in increased gel strength in the concentrate food composition according to the invention, resulting at the end of the process and no additional gelling agent needs to be added like starch or gums (e.g. pectin) .
• the pH reduction can be achieved, as known in the art, by using a suitable food grade acid such as for example hydrochloric acid, citric acid, phosphoric acid or mixtures thereof.
• a suitable food grade acid such as for example hydrochloric acid, citric acid, phosphoric acid or mixtures thereof.
• the temperature is preferably kept between 30°C and 90°C, more preferably between 40°C and 80°C, most preferably between 50°C and 75°C.
• this acidification step it maybe preferred to adjust, after the acidification step, the pH to the pH as optimal for PME enzyme which is used in step b) .
• the invention relates to a process comprising the steps of:
• process further comprises the step of:
• the process of the invention further comprises the step of adding pectin with a degree of esterification of below 55% and which is not bound to plant material, e.g. not bound to comminuted plant material, herein defined as active free pectin (AFP) .
• This step is carried out preferably before addition of the sodium salt (i.e. before step d) and more preferably before the addition of PME (i.e. before step b) .
• This step can be preferred in addition to the acidification step as described above or for example instead of this acidification step.
• This step may for example be preferred in case frozen or defrosted plant material is used to prepare comminuted plant material. Accordingly, a process may be preferred comprising the steps of:
• process further comprises the step of:
• the process of the invention may comprise the step of adding free, inactive pectin (not bound to plant material, e.g. not bound to comminuted plant material) .
• This free inactive pectin is added before the addition of PME in step b) .
• This step can be preferred in addition to the acidification step as described above, or in addition to the step of adding additional free active pectin, as described above, or instead of these steps.
• This step may for example be preferred in case frozen or defrosted plant material is used to prepare comminuted plant material.
• the invention relates to a process comprising the step of adding pectin with a degree of
• process further comprises the step of:
• the invention further relates to a concentrate food composition obtainable by the process of the present invention.
• concentrate food composition of the present invention is preferably a bouillon concentrate, a soup concentrate, a sauce concentrate a gravy concentrate or a seasoning concentrate.
• the present invention relates to the use of the concentrate food composition according to the invention to prepare a bouillon, a soup, sauce, gravy or a seasoned dish.
• the use according to the present invention preferably comprises diluting the concentrate food composition according to the invention, at least part of it, in an aqueous liquid, or mixing it into a dish.
• the aqueous liquid is preferably water, but can be a sauce, a soup, milk etc.
• the dish can be a vegetable dish, meat, fowl, fish etc.
• the temperature of the aqueous solution or dish is preferably of between 60°C and 100°C, more preferably of between 70°C and 95°C.
• the food composition according to the invention disperses relatively fast in hot water (e.g. 95°C) . It may be preferred that 25 gram (more or less cube-shaped) disperses in 1L of hot water within 3
• a Dispersion Test can be used where the mass decrease of a semi-solid gel over time is measured. This mass decrease is caused by breaking apart of said large semi-solid gel into smaller particles.
• the food composition according to the invention disperses relatively fast in hot water (e.g. 95°C) . It may be preferred that 25 gram of a cube-shaped gel disperses in 1L of hot water within 3 minutes, preferably within 2 minutes.
• the temperature of the machine is set to 95-97°C. •
• the gel sample is inserted into the food
• the product is sieved (water + gel food composition) through a 1 mm mesh sieve.
• Measurements are taken after a period of dispersion of, for example, 15s, 30s, lmin, 2min, 4min, 8min at least in duplicates .
• a dispersion time is chosen which is sufficiently long enough to essentially disperse the entire gel product.
• the weight of the material which is left in the sieve is not of gel particles but of other particles which were present in the original gel product, such as vegetable particles, meat pieces etc.
• a dispersion time of 10 min was sufficient to disperse the entire gel product and
• • min is the weight of product (particles) left in the sieve after 10 min, i.e. when the entire gel product has been dispersed) .
• Samples are dispersed (e.g. lOx dilution) at a
• the solutions are transferred into a rheometer (for example, MCR300 or MCR 301 Physica, Anton Paar) pre ⁇ heated at 90-85°C and equipped with a profiled cylinder and bob.
• a rheometer for example, MCR300 or MCR 301 Physica, Anton Paar
• a high salt savoury food concentrate could be obtained in the form of semi-solid gel.
• the concentrate food composition could be prepared starting from fresh plant material, without the need for additional gelling agents, providing a perception of naturalness.
• the concentrate food composition did not show any significant undesirable increase in viscosity after dilution in water and subsequent cooling down. The appearance in the application (i.e. in use) therefore was strongly improved compared to conventional concentrate food compositions.
• the concentrate food composition according to the invention showed an easy and natural spoonability, for example when compared to the concentrate food compositions currently available, wherein for example xanthan and locust bean or xanthan and iota- carrageenan is used as a gelling agent.
• a concentrate food composition was prepared according to the invention .
• the cut onions were heated by using a microwave oven to reach a temperature of above 95°C.
• An acidification step was carried out by lowering of the pH to 1.6 with 6M HC1.
• the pH was kept at 1.6 for 10 minutes at a temperature of 65 to75°C, and was then adjusted to pH 4.1 using 6M NaOH.
• the PME was inactivated by a heating step to above 80°C using a microwave oven
• the product was a gel at room temperature
• the resulting product was removed from the packaging and checked for shape stability by eye. The firmness, brittleness, and recovery of the gels were measured according to the method as described in the description.
• the product according to the invention was compared with a product produced by a different production process (Comparative example lb and Id) , wherein a heated and a non-heated fraction of comminuted vegetable are combined. This production process did not result in a semi-solid gel.
• a) Preparation of a concentrate product using a different production process.
• the cut onions were heated using a microwave oven to reach a temperature of above 95°C.
• comparative examples lb and Id confirmed the visual observation that the texture was not a semi-solid gel, but a paste.
• a gel was prepared according to the invention.
• a comparative example was prepared, without a PME treatment.
• the product treated with PME formed a shape stable gel (2a and 2b) .
• the product incubated without added PME (2c) did not form a shape stable gel, but a weak paste.
• the firmness was up to 12x higher for the products with PME treatment at 15% wt NaCl compared to the product which was not PME treated (sample 2a and 2b, both PME treated compared with sample 2c in which no PME treatment was not done) .
• Example 3 The products from example 2a and 2b were easy to spoon out and did not show undesired increase in viscosity upon cooling after dilution.
• Example 3 The products from example 2a and 2b were easy to spoon out and did not show undesired increase in viscosity upon cooling after dilution.
• b 'Savoury mix contains sugar, yeast extract, meat powder base, parsley, pepper, flavours. Also contains 7.6% Na + , 3.1% K + and 0.1% Ca 2+ .
• Example 3 shows that in this example a PME treatment is
• a shape stable gel was prepared using carrots as starting material .
• Carrots were scratched from outside (peeled) and cut in small pieces (1-3 cm slices) .
• An acidification step was carried out by lowering of the pH to 1.6 with 6M HC1.
• the pH was kept at 1.6 for 10 minutes at a temperature of 65 to75°C, and was then adjusted to pH 4.1 using 6M NaOH.
• step 3 The water added in step 3 was evaporated in this step.
• the end product was hot filled in jars and stored at 5°C and allowed to form a gel.
• the gels were easy to spoon (not sticky, clear cut piece) and no undesired increase in viscosity upon cooling after dispersion in hot water was observed. The gels were easy to disperse in hot water.
• sunflower oil was added and then mixed in constantly at high speed for 2 min using a kitchen blender and a comminuted onion puree was
• An acidification step was carried out by lowering of the pH to 1.6 with 6M HC1. The pH was kept at 1.6 for 10 minutes at a temperature of 65 to75°C, and was then adjusted to pH 4.1 using 6M NaOH.
• the product was hot filled in jars, stored at 5°C and allowed to form a gel.
• the resulting gel was analysed using the protocol as described in the description.
• hydrocolloid was required.
• the obtained gel was brittle and allowed good spoonability .
• the product was easy to disperse in hot water and did not show undesirable increase in viscosity upon cooling.
• Example 7 Analysis of viscosity increase upon cooling of the diluted product
• Example 7a a food concentrate in the form of a gel according to the invention was compared to a food concentrate in the form of a gel from the prior art, using a the combination of xanthan and locust bean gum as a gelling system (comparative example7b) .
• Example 7a was prepared as described for Example
• Comparative example 7b contains:
• high-salt food concentrates according to this invention (example 7a, lc) , compared to, were more brittle and easier to scoop/spoon and faster to disperse while stirring upon dilution in hot water, compared to comparative example 7b.

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• 2013
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