WO2013180528A1 - 정확도가 향상된 전기화학적 바이오센서 - Google Patents
정확도가 향상된 전기화학적 바이오센서 Download PDFInfo
- Publication number
- WO2013180528A1 WO2013180528A1 PCT/KR2013/004838 KR2013004838W WO2013180528A1 WO 2013180528 A1 WO2013180528 A1 WO 2013180528A1 KR 2013004838 W KR2013004838 W KR 2013004838W WO 2013180528 A1 WO2013180528 A1 WO 2013180528A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- electrode
- electrochemical biosensor
- working electrode
- auxiliary electrode
- measuring
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
Definitions
- the present invention relates to a biosensor which improves the accuracy of measurement by giving an electrochemical biosensor a function capable of correcting the effects of blood samples, in particular the effect of hematocrit.
- the blood glucose measurement can be easily measured using a hand-held measuring instrument, and specifically, each patient can be easily measured using a strip-type biosensor.
- the operating principle of the biosensor for measuring blood glucose is based on a colorimetric method or an electrochemical method.
- This enhanced electrochemical method is described by Reaction 1 below, and the biggest feature is the use of an electron transfer medium.
- the electron transfer medium include ferrocene (ferrocene) and ferrocene derivatives; Quinones, quinone derivatives; Transition metal-containing organic and inorganic materials (nuxaamine ruthenium, osmium-containing polymer, potassium ferricyanide, etc.); And organic conducting salts, electron transfer organics such as viologen, and the like.
- GOx represents Glucose oxidase
- GOx-FAD and G0x-FADH 2 represent the oxidation state and reduction state of FAD (flavin adenine dinucleotide), which are the active sites of glycase, respectively.
- FAD flavin adenine dinucleotide
- glucose in the blood is first oxidized to gluconic acid by the catalysis of glycosylase.
- FAD which is an active site of glycosylase is reduced to FADH 2 .
- the electron transfer mediator is to be reduced.
- This electron transfer mediator in the reduced state is formed in the diffusion to the electrode surface, this time by measuring the current generated by applying an "oxidation potential of the electron transfer mediator in reduced state on the working electrode surface is measured, the concentration of blood glucose.
- sugar dehydrogenases such as GDH-FAD may be used instead of the glycosylase of GOx-FAD. Even if there is a difference in the type of enzyme, the overall reaction follows the process of reaction 1 above.
- a biosensor using the above electrochemical method as an operating principle is called an electrochemical biosensor.
- the biochemical biosensor has a merit that it is possible to reduce the influence of oxygen, unlike the conventional biosensor by the colorimetric method, and the sample can be used without any pretreatment even if the sample is turbid.
- These sensors are generally conveniently used to control blood glucose levels, but the accuracy of the sensors is present in the blood sample. Is strongly influenced by various interfering species such as ascorbic acid, acetaminophene and uric acid.
- the erythrocyte volume ratio ratio of red blood cells in the whole blood; Hematocrit
- Hematocrit is important as a factor causing serious error in the measurement accuracy of the electrochemical biosensor.
- Patent Documents 1 and 2 disclose a method of separating red blood cells or applying a layer for removing red blood cells on a reagent layer.
- Patent Literature 3 discloses a method of using a peritoneal membrane having a reagent / blood cell separation integrated function capable of screen printing including a silicon filler.
- Patent document 4 discloses a calibration method in which the results obtained by applying the applied excitation potentials twice are mathematically processed (chemometric method).
- an electrode for measuring erythrocyte volume directly by electric conductivity or resistance is separately provided with an electrode for measuring enzyme reaction, and the erythrocyte volume ratio is measured separately. Using this result, the glucose concentration obtained from the enzyme reaction electrode is corrected to correct the measurement accuracy. Can increase.
- the method of measuring the erythrocyte volume ratio as the conductivity of the disposable electrode equipped with the working electrode and the auxiliary electrode mounted on the capillary sample cell has already been proposed by the existing technology (Patent Document 5).
- Patent Document 6 There is also an applied example (Patent Document 6).
- the present invention focuses on the fabrication of a sensor capable of accurately measuring conductivity during mass production of the sensor, and the electrical conductivity (G) of blood is based on the following formula (1).
- G is the electrical conductivity of unit ⁇ — 1
- ⁇ is the conductivity coefficient of the blood unit of ⁇ ⁇ 1 cm "1
- A is the area of the electrode of unit cm 2
- L is the unit Therefore, the distance between electrodes and the area of the electrode are important for accurate conductivity measurement.
- the area of the conductivity measurement electrode and the electrode for the measurement of 5 hematocrit in the mass production biosensor are shown.
- the electrodes are often configured through a printing method, which is not printed exactly as desired depending on the composition of the electrode. The edge slope tends to flow down so that there is a tendency for deviation in the area of the electrode and the distance between the electrodes.
- the electrochemical bio including a first electrode portion for measuring the red blood cell volume ratio correction value and a second electrode portion for measuring the glucose concentration
- the area of the first working electrode and the first auxiliary electrode of the first electrode portion exposed to the blood sample is not only identical by providing an insulating cover that is larger than the thickness of the working electrode and the auxiliary electrode, and a position error occurs when printing the electrodes. Even though the same electrode area is always maintained by the insulating cover, it has been found that the accuracy of the red blood cell volume ratio measurement is improved and the present invention has been completed.
- Patent document 1 JP 2000338076 A
- Patent Document 2 US 5658444 Al
- Patent Document 3 us 6241862 Bl
- Patent Document 4 wo 01/57510 A2
- Patent Document 5 us 4301412 Al
- Patent Document 6 us 20110139634 Al
- An object of the present invention is to provide an electrochemical biosensor for blood glucose measurement with improved red blood cell volume measurement accuracy.
- ⁇ ⁇ ⁇ L ⁇ ti ⁇ ⁇ Great phase——Vassel——Red stone—Provides a method for precisely measuring blood glucose levels by testing red blood cell volume levels. will be.
- a first electrode part including a first working electrode and a first auxiliary electrode formed on the lower plate;
- a second electrode unit including a second working electrode and a second auxiliary electrode formed on the lower plate;
- a reagent layer comprising an electron transfer medium formed on the second working electrode or the second auxiliary electrode;
- Areas corresponding to the first semi-aperture part positioned in the first working electrode, the second semi-recessed part located in the first auxiliary electrode, and the third semi-recessed part located in the second working electrode and the second auxiliary electrode are respectively pupilized to form a zone. Insulation cover to distinguish;
- An intermediate substrate having a micro channel sample cell part for sequentially inducing blood samples to the first electrode part and the second electrode part;
- the insulating cover provides an electrochemical biosensor for measuring blood glucose, characterized in that the area of the first working electrode and the first auxiliary electrode exposed from the first and second reaction parts and the second auxiliary electrode are constant.
- the present invention using the biosensor,
- step 1 Introducing a blood sample into the sample cell unit with a micro flow path (step 1);
- Step 4 It provides a method for precisely measuring the blood glucose concentration by reflecting the erythrocyte volume fraction value, including.
- FIG. 1 is a first working electrode and a first auxiliary electrode exposed to the blood sample in the electrochemical biosensor according to the first embodiment of the present invention is located at right angles to the blood flow direction, between the area of these electrodes and the electrode
- FIG. 2 is an enlarged view of an electrode part of FIG. 1.
- Figure 3 is an enlarged view showing that the area of the first working electrode and the first auxiliary electrode exposed to the blood sample in the electrochemical biosensor according to Comparative Example 1 of the present invention is not defined by the insulating cover.
- FIG. 5 is an exemplary view illustrating corner slopes of the first working electrode and the first auxiliary electrode in FIG. 1.
- FIG. 6 is a portion of the electrode defined by the insulating cover as shown in FIG. The part is photographed with a microscope.
- FIG. 7 is a photograph of a microscopic view of a portion of the electrode not defined by the insulating cover as in FIG. 3.
- Example 8 is a graph showing the results of measuring the electrical conductivity of the red blood cell volume using the sensor manufactured in Example 1 (wherein "% Hct” means% red blood cell volume ratio).
- the present invention Lower plate
- a first electrode part comprising
- a second electrode portion comprising;
- a reagent layer comprising an electron transfer medium formed on the second working electrode or the second auxiliary electrode;
- the areas of the first semi-aperture part located in the first working electrode, the second reaction part located in the first auxiliary electrode, and the third semi-recessed part located in the second working electrode and the second auxiliary electrode are respectively pupild to form a zone. Insulation cover to distinguish;
- An intermediate substrate having a micro channel sample cell part for sequentially inducing blood samples to the first electrode part and the second electrode part;
- the insulating cover provides an electrochemical biosensor for measuring blood glucose, characterized in that the area of the first working electrode and the first auxiliary electrode exposed from the first reaction part and the second reaction part and the electrode spacing are constant (FIG. 1). And FIG. 2).
- the lower plate serves as a substrate of the biosensor.
- the material of the lower plate may be a ceramic, glass plate or a polymer material, preferably an organic polymer material such as polyester, polyvinyl chloride (polycarbonate) and polycarbonate (polycarbonate) may be used.
- the first electrode part serves to test the hematocrit value by measuring the electrical conductivity of the blood sample. In general, a very accurate measurement of glucose concentration is required for the prescription of diabetic patients, and since the error occurs in the measurement of glucose concentration according to the blood red blood cell volume level of the patient, it is necessary to correct this.
- the first electrode unit includes a first working electrode and a first auxiliary electrode, and the red blood cell volume in the blood sample is measured with an electrical conductivity measured by applying an alternating voltage (AC) of 1 kHz or more, This measurement corrects blood sample glucose concentrations to allow for more accurate blood glucose levels.
- the second electrode portion serves to measure the glucose concentration of the blood sample.
- the second electrode part includes a second working electrode and a second auxiliary electrode, and measures a blood sample glucose concentration by applying a DC voltage or the like.
- the material of the electrode used in the biosensor according to the present invention is not particularly limited as long as it is a conductive material. Examples of conductive materials include silver epoxy palladium, copper, gold, platinum, iridium, silver / silver chloride, carbon and modified carbon reinforced with certain redox pairs or other additives.
- the working electrode and the auxiliary electrode may be formed by screen printing a conductive material on a substrate, physical vapor deposition or etching, or by attaching a conductive tape.
- the reagent layer including the electron transfer medium may further include an oxidoreductase.
- the oxidoreductase is reduced by reacting with the glucose to be measured, and the reduced enzyme reacts with the electron transfer medium to quantify glucose.
- oxidoreductases Flavin adenine dinucleot i de-glucose dehydrogenase (FAD-GDH), nicotinamide adenine dinucleot ide ⁇ glucose 'dehydrogenase, NAD-GDH Roloquinoline quinone-glucose dehydrogenase (Pyrroloquinol ine quinone-glucose dehydrogenase, PQQ-GDH), glucose oxidase (GOx), etc. can be used.
- the electron transfer mediator reacts with glucose to reduce the reduced enzyme and redox reaction.
- the electron transfer mediator thus formed serves to generate current at the electrode surface to which the oxidation potential is applied.
- a metal-containing complex and thionine or a derivative thereof may be mixed and used together, but a conventionally used nucleated hexaamine chloride 3 ⁇ 4- (HI) (hexaamminerut hen i urn ( ⁇ ) chloride),
- the area of the first working electrode and the first auxiliary electrode exposed to the blood sample using the insulating cover is as constant as possible and L ⁇ work between these electrodes.
- ⁇ ⁇ — ⁇ ⁇ ⁇ — also the lower side of the lower side of the lower side of the car 7 More specifically, the thickness of the insulating cover is from the upper surface of the lower plate to the upper surface of the first working electrode and the first auxiliary electrode. It is formed thinner than the thickness to be measured, so it is possible Can be minimized (see FIG. 5).
- a method of printing with a hydrophobic insulating ink can be used.
- hydrophobic insulating ink hydrophobic inks such as polyacrylic, epoxy and ceramic series may be used.
- the first half abutment part positioned in the first working electrode and the second half abutment part positioned in the first auxiliary electrode have the same shape, and may be provided in the form of a rectangle, a square, a circle, an oval, or the like. Rectangular or square is preferred for the convenience of the manufacturing process.
- the first reaction part located in the first working electrode and the second reaction part located in the first auxiliary electrode are positioned on a line perpendicular to the longitudinal direction in which blood flows into the micro-channel sample cell part, based on the longitudinal center line. It can be located in the form of decalcomani.
- the intermediate substrate is ⁇ i ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ and topic-2-nationwide- ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ It is to be introduced and the adhesive is coated on both sides thereof serves to bond the upper and lower plates.
- the size of the micro-channel sample portion is 0.5- 2 ⁇ in width, 50-250 ⁇ micro-channel having a height of which is formed, blood sample It is preferable from the viewpoint of facilitating introduction.
- the upper plate is provided with an air outlet port to serve as a blood sample through the capillary phenomenon well through the micro-channel sample cell and serves as a finishing material of the biosensor.
- the present invention provides a method for precisely measuring the blood glucose concentration by reflecting the erythrocyte volume fraction value using the biosensor comprising the following steps:
- step 1 Introducing a blood sample into the sample cell unit with a micro flow path (step 1);
- step 1 is a step of introducing a blood sample into the sample sal portion with a micro flow path.
- step 2 is a step of calculating the value of red blood cell volume by measuring the electrical conductivity of the blood sample in the first electrode part
- step 3 is measuring the glucose concentration by measuring a signal at the second electrode part. Calculation step.
- direct current, low frequency or high frequency alternating current, high impedance or various types of fields preferably rectangular waves, triangular waves, semi-sinusoidal waves, Gaussian waves, etc. are used between the working electrode and the auxiliary electrode of the first electrode portion and the second electrode portion. To control the entire measurement process.
- a predetermined constant AC voltage is applied between the working electrode and the auxiliary electrode of the first electrode part and / or the second electrode part.
- the voltage applied to the actuating electrode and the auxiliary electrode is independent, and the entire furnace forms an open circuit.
- the electrical change caused by the sample injection appears as a potential difference in an open circuit state, and the potential difference signal is used as a start signal of the measurement process of the biosensor.
- the reagent layer including the electron transfer medium is formed on only one electrode of the second working electrode or the second auxiliary electrode, the interval between the electrodes is preferably arranged in the range of 20 ⁇ to 2 mm, more Preferably the electrode spacing may be arranged in the range of 80 im to 0.5 mm.
- the reagent layer may further include an oxidoreductase,
- the redox enzymes include flavin adenine dinucleot i de-glucose dehydrogenase (FAD-GDH) and nicotinamide adenine dinucleotide-.
- FAD-GDH flavin adenine dinucleot i de-glucose dehydrogenase
- nicotinamide adenine dinucleotide- Glucose dehydrogenase (nicot inamide adenine dinucleot i de-glucose dehydrogenase, NAD-GDH), pyrroloquinoline quinone (glucose dehydrogenase PQQ-GDH), glucose oxidase (GOx), etc.
- the electron transporting medium a metal-containing complex and thionine or a derivative thereof may be mixed and used together, but conventionally used hexaammineruthenium (III) chlor ide),
- Potassium ferricyanide (potassium f er ri cyani de), potassum ium f errocyani de, dimethylferrocene (DMF), ferr icinium, ferrocenemono ⁇ "carboxylic acid (ferocene monocarboxyl ic acid (FCOOH)), 7,7,8,8, -tetracyanoquinodimethane (7,7,8,8-tetracyanoqui no-dimethane (TCNQ)), tetrathiafulvalene (t et rathi a fulvalene (TTF)), nickellocene (Nc), N-methyl asydinium (N-methy 1 acidinium ( ⁇ A +)), tetrathiatetracene (TTT), N-methyl Phenazinium (N-methylphenazinium (NMP +)), hydroquinone, 3-dimethylaminobenzoic acid (MBTHDMAB), 3-methyl ⁇
- step 4 is a step of correcting by reflecting the erythrocyte volume fraction calculated by the first electrode to the glucose concentration calculated by the second electrode.
- the measurement order of the first electrode portion and the second electrode portion is not limited.
- the erythrocyte volume ratio measured by the erythrocyte volume ratio correction formula previously input to the measuring instrument is calculated using the conductivity measurement value measured at the first electrode unit, and the blood glucose measurement value measured at the second electrode unit is previously input to each erythrocyte.
- the volume is calculated using the correction formula to calculate the red blood cell volume ratio and the influence, and the accurate measurement value is displayed.
- the electrochemical biosensor comprising a first electrode portion for red blood cell volume measurement correction and a second electrode portion for measuring glucose concentration, the insulating cover thinner than the thickness of the working electrode and the auxiliary electrode
- the insulating cover thinner than the thickness of the working electrode and the auxiliary electrode
- Detailed method for manufacturing the flat bar ion sensor is Korean Patent Application No. 1 () ⁇ 2003-0036804, Korean Patent Application No. 10-2005-0010720 Korean Patent Application No. 10-2007-0020447 Korean Patent Application 10-2007-0021086 , Korean Patent Application No. 10-2007-0025106, Korean Patent Application No. 10—200 No. 0030346, EK Bauman et al. , Analytical Chemistry, vol 37, p 1378, 1965 KB Oldham in "Mi croe 1 ect rodes Theory and Appli c ions," Kluwer Academic Publ i shers, 1991.
- FIG. 1
- 1 is a lower plate made of polyester in which the working electrode and the auxiliary electrode are formed,
- 2 to 5 are electrodes made by screen printing carbon / graphite, 2 is a first working electrode, 3 is a first auxiliary electrode, 4 is a second working electrode, 5 is a second auxiliary electrode,
- 6 is the first electrode portion and the second electrode portions defined, and the first electrode, and the insulating cover to the same rules of the first working electrode and the first area of the auxiliary electrode portion
- 7 is a redox enzyme and an electron transfer mediator Reagent layer included
- top plate 9 is a top plate made of polyester with an air outlet for blood to penetrate into the sample cell portion with a fine flow path
- a biosensor was manufactured in the same manner as in Example 1, but a flat type biosensor was manufactured in which the insulation cover 6 was not located between the first and second working electrodes.
- a biosensor is manufactured in the same manner as in Example 1, wherein the insulating cover 6 is sequentially positioned with respect to the direction in which blood flows into the first working electrode and the second working electrode.
- a flat type biosensor not specified by this insulating cover was manufactured.
- Example 8 is a graph showing the result of measuring the electrical conductivity with respect to the red blood cell volume using the sensor manufactured in Example 1 (wherein, “Met” means% red blood cell volume ratio).
- 9 is a graph showing the results of measuring the electrical conductivity with respect to the red blood cell volume using the sensor manufactured in Comparative Example 1 (wherein, “% Hct” means 3 ⁇ 4> red blood cell volume ratio).
- FIG. 10 is a graph showing the result of measuring the electrical conductivity with respect to the red blood cell volume ratio using the sensor manufactured in Comparative Example 2 (wherein, "% Hct" means 3 ⁇ 4 red blood cell volume).
- % Hct means 3 ⁇ 4 red blood cell volume.
- the slope of the conductivity per% erythrocyte volume fraction was '-0.0008' in Example 1, was measured as ' ⁇ 0.0011' in Comparative Example 1, which is the first working electrode and the first This is because the distance dl between the auxiliary electrodes is longer than the distance d2 in Comparative Example 1 (see formula (1) in the background art).
- Comparative Example 2 is not a preferred structure in terms of convenience of the user because the amount of blood required for the measurement is increased.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Diabetes (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Emergency Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015514916A JP5873215B2 (ja) | 2012-06-01 | 2013-05-31 | 向上した精度を備えた電気化学的バイオセンサ |
CN201380031465.7A CN104380095B (zh) | 2012-06-01 | 2013-05-31 | 具有改进的精度的电化学生物传感器 |
EP13796996.0A EP2871470B1 (en) | 2012-06-01 | 2013-05-31 | Electrochemical biosensor with improved accuracy |
US14/556,891 US9753004B2 (en) | 2012-06-01 | 2014-12-01 | Electrochemical biosensor with improved accuracy |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020120059327A KR101466222B1 (ko) | 2012-06-01 | 2012-06-01 | 정확도가 향상된 전기화학적 바이오센서 |
KR10-2012-0059327 | 2012-06-01 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/556,891 Continuation US9753004B2 (en) | 2012-06-01 | 2014-12-01 | Electrochemical biosensor with improved accuracy |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2013180528A1 true WO2013180528A1 (ko) | 2013-12-05 |
Family
ID=49673647
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2013/004838 WO2013180528A1 (ko) | 2012-06-01 | 2013-05-31 | 정확도가 향상된 전기화학적 바이오센서 |
Country Status (6)
Country | Link |
---|---|
US (1) | US9753004B2 (ko) |
EP (1) | EP2871470B1 (ko) |
JP (1) | JP5873215B2 (ko) |
KR (1) | KR101466222B1 (ko) |
CN (1) | CN104380095B (ko) |
WO (1) | WO2013180528A1 (ko) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105510391A (zh) * | 2014-09-22 | 2016-04-20 | 英科新创(厦门)科技有限公司 | 一种电极式血糖试条 |
WO2016145721A1 (zh) * | 2015-03-14 | 2016-09-22 | 深圳市贝沃德克生物技术研究院有限公司 | 用于血糖仪试纸针的试纸条 |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150140671A1 (en) * | 2013-11-18 | 2015-05-21 | Johnson Electric S.A. | Method and system for assembling a microfluidic sensor |
KR101671456B1 (ko) * | 2014-07-11 | 2016-11-16 | 최강 | 바이오센서 |
KR20160014212A (ko) * | 2014-07-28 | 2016-02-11 | 주식회사 아이센스 | 휴대용 혈당 측정기 |
KR101666978B1 (ko) | 2014-09-17 | 2016-10-24 | 주식회사 아이센스 | 생체시료 내 분석대상물질의 농도측정방법 및 측정장치 |
JP6206621B2 (ja) * | 2015-04-15 | 2017-10-04 | 株式会社村田製作所 | 呼気センサ、呼気センサユニットおよび呼気検出方法 |
GB201511299D0 (en) * | 2015-06-26 | 2015-08-12 | Inside Biometrics Ltd | Test device and method of using a test device |
TWI579562B (zh) * | 2015-11-11 | 2017-04-21 | Method of Making Electrochemistry Test Specimen | |
MX2018007386A (es) * | 2015-12-18 | 2018-11-09 | Trividia Health Inc | Sensor in-vitro que utiliza una medicion de impedancia tetrapolar. |
US10302587B2 (en) * | 2016-03-29 | 2019-05-28 | Broadmaster Biotech Corp. | Test strip and method of operation thereof |
KR101875417B1 (ko) * | 2016-07-01 | 2018-07-10 | (주)오상헬스케어 | 바이오센서 |
TWI589869B (zh) * | 2016-08-08 | 2017-07-01 | 友達光電股份有限公司 | 感測裝置及該感測裝置之電極試片 |
US11467117B2 (en) * | 2017-07-19 | 2022-10-11 | Sharp Kabushiki Kaisha | Sensor array |
KR102336969B1 (ko) * | 2017-07-20 | 2021-12-08 | 동우 화인켐 주식회사 | 글루코스 센서 |
KR102459679B1 (ko) * | 2017-09-19 | 2022-10-27 | 동우 화인켐 주식회사 | 글루코스 센서 |
KR102486571B1 (ko) * | 2017-10-24 | 2023-01-09 | 주식회사 미코바이오메드 | 바이오 측정 장치 |
US11344878B2 (en) * | 2018-07-06 | 2022-05-31 | Qorvo Us, Inc. | Fluidic channels including conductivity sensor and methods of use thereof |
KR102179203B1 (ko) * | 2018-07-09 | 2020-11-16 | 주식회사 필로시스 | 혈당 센싱 데이터 판별 방법 및 장치 |
CN109061190B (zh) * | 2018-08-22 | 2021-12-07 | 苏州微湃医疗科技有限公司 | 基于纸芯片的多通道生物传感器阵列制备及免疫检测应用 |
CA3199825A1 (en) | 2018-11-02 | 2020-02-07 | Cardiai Technologies Ltd. | Portable electrochemical-sensor system for analyzing user health conditions and method thereof |
KR102235310B1 (ko) | 2019-05-16 | 2021-04-01 | 단국대학교 천안캠퍼스 산학협력단 | 키토산-탄소나노튜브 코어-쉘 나노하이브리드 기반의 전기화학 글루코즈 센서 |
AU2020327164B2 (en) * | 2019-08-02 | 2023-02-23 | Bionime Corporation | Micro biosensor and method for reducing measurement interference using the same |
CN115078508B (zh) * | 2022-06-21 | 2024-01-19 | 爱森斯(江苏)生物科技有限公司 | 电化学生物传感器及其制备方法 |
CN116026906A (zh) * | 2022-12-29 | 2023-04-28 | 苏州和林微纳科技股份有限公司 | 一种微流控血糖检测芯片 |
CN115825178B (zh) * | 2023-01-11 | 2024-07-19 | 宁波康和长晟科技有限公司 | 一种基于自支撑葡萄糖电催化材料的电化学葡萄糖传感器 |
Citations (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4301412A (en) | 1979-10-29 | 1981-11-17 | United States Surgical Corporation | Liquid conductivity measuring system and sample cards therefor |
US5658444A (en) | 1993-05-12 | 1997-08-19 | Medisense, Inc. | Electrochemical sensors |
JP2000338076A (ja) | 1999-05-25 | 2000-12-08 | Matsushita Electric Ind Co Ltd | バイオセンサ |
US6241862B1 (en) | 1996-02-14 | 2001-06-05 | Inverness Medical Technology, Inc. | Disposable test strips with integrated reagent/blood separation layer |
WO2001057510A2 (en) | 2000-02-02 | 2001-08-09 | Lifescan, Inc. | Electrochemical methods and devices for use in the determination of hematocrit corrected analyte concentrations |
US6287451B1 (en) * | 1999-06-02 | 2001-09-11 | Handani Winarta | Disposable sensor and method of making |
KR20030036804A (ko) | 2000-09-20 | 2003-05-09 | 가부시키가이샤 미쿠니 | 연료공급장치 및 이 연료공급장치에 사용되는 연료필터 |
KR20050010720A (ko) | 2004-09-24 | 2005-01-28 | 한상배 | 자흡기능과 교반기능이 구비된 유체공급장치와 이를이용한 포기장치 |
JP2006504096A (ja) * | 2002-10-23 | 2006-02-02 | アボット・ラボラトリーズ | ヘマトクリット及び酸素バイアスを有するバイオセンサ |
KR100586832B1 (ko) * | 2004-08-27 | 2006-06-08 | 주식회사 인포피아 | 바이오센서의 시료반응결과 측정장치 |
KR20070020447A (ko) | 2006-11-07 | 2007-02-21 | 지. 톰프슨 로저 | 즉각적으로 착용할 수 있는 표식을 가진 모자 챙 성형 장치 |
KR20070021086A (ko) | 2005-08-17 | 2007-02-22 | 가부시키가이샤 고베 세이코쇼 | 소스/드레인 전극, 박막 트랜지스터 기판, 그의 제조방법,및 표시 디바이스 |
KR20070025106A (ko) | 2005-08-31 | 2007-03-08 | 엘지전자 주식회사 | 멀티형 공기조화기의 압축기 오일 회수장치 |
KR20070030346A (ko) | 2005-09-13 | 2007-03-16 | 전달용 | 수익 자동 배분 방법 및 시스템 |
US20090056120A1 (en) * | 1999-10-04 | 2009-03-05 | Bhullar Raghbir S | Biosensor and method of making |
US20100219084A1 (en) * | 2006-10-05 | 2010-09-02 | Stephen Patrick Blythe | Method for determining hematocrit corrected analyte concentrations |
US20110139634A1 (en) | 2009-12-14 | 2011-06-16 | Taidoc Technology Corporation | System and method for measuring analyte concentration with interferant correction |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3810534B2 (ja) * | 1997-10-17 | 2006-08-16 | 松下電器産業株式会社 | ヘマトクリット値測定用素子およびヘマトクリット値の測定方法 |
US7073246B2 (en) * | 1999-10-04 | 2006-07-11 | Roche Diagnostics Operations, Inc. | Method of making a biosensor |
EP2202511A3 (en) * | 2001-01-17 | 2010-09-29 | ARKRAY, Inc. | Quantitative analyzing method and quantitative analyzer using sensor |
US6837976B2 (en) * | 2002-04-19 | 2005-01-04 | Nova Biomedical Corporation | Disposable sensor with enhanced sample port inlet |
KR100554649B1 (ko) * | 2003-06-09 | 2006-02-24 | 주식회사 아이센스 | 전기화학적 바이오센서 |
US7452457B2 (en) * | 2003-06-20 | 2008-11-18 | Roche Diagnostics Operations, Inc. | System and method for analyte measurement using dose sufficiency electrodes |
PL1642124T3 (pl) * | 2003-06-20 | 2018-04-30 | F.Hoffmann-La Roche Ag | Biosensory elektrochemiczne |
US8535497B2 (en) * | 2003-12-04 | 2013-09-17 | Panasonic Corporation | Method of measuring blood component, sensor used in the method, and measuring device |
KR100698961B1 (ko) * | 2005-02-04 | 2007-03-26 | 주식회사 아이센스 | 전기화학적 바이오센서 |
US7611621B2 (en) * | 2005-06-13 | 2009-11-03 | Nova Biomedical Corporation | Disposable oxygen sensor and method for correcting oxygen effect on oxidase-based analytical devices |
US8617366B2 (en) * | 2005-12-12 | 2013-12-31 | Nova Biomedical Corporation | Disposable urea sensor and system for determining creatinine and urea nitrogen-to-creatinine ratio in a single device |
KR100890988B1 (ko) * | 2007-10-29 | 2009-03-31 | 주식회사 아이센스 | 일정 소량의 시료를 균일하게 도입할 수 있는 시료도입부를구비한 전기화학적 바이오센서 |
JP2010230369A (ja) * | 2009-03-26 | 2010-10-14 | Ryukoku Univ | 電極構造及び当該電極構造の製造方法並びに電気化学センサ |
KR101979680B1 (ko) | 2012-12-05 | 2019-05-20 | 삼성전자주식회사 | 촉각센서 |
-
2012
- 2012-06-01 KR KR1020120059327A patent/KR101466222B1/ko active IP Right Grant
-
2013
- 2013-05-31 WO PCT/KR2013/004838 patent/WO2013180528A1/ko active Application Filing
- 2013-05-31 EP EP13796996.0A patent/EP2871470B1/en active Active
- 2013-05-31 JP JP2015514916A patent/JP5873215B2/ja active Active
- 2013-05-31 CN CN201380031465.7A patent/CN104380095B/zh active Active
-
2014
- 2014-12-01 US US14/556,891 patent/US9753004B2/en active Active
Patent Citations (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4301412A (en) | 1979-10-29 | 1981-11-17 | United States Surgical Corporation | Liquid conductivity measuring system and sample cards therefor |
US5658444A (en) | 1993-05-12 | 1997-08-19 | Medisense, Inc. | Electrochemical sensors |
US6241862B1 (en) | 1996-02-14 | 2001-06-05 | Inverness Medical Technology, Inc. | Disposable test strips with integrated reagent/blood separation layer |
JP2000338076A (ja) | 1999-05-25 | 2000-12-08 | Matsushita Electric Ind Co Ltd | バイオセンサ |
US6287451B1 (en) * | 1999-06-02 | 2001-09-11 | Handani Winarta | Disposable sensor and method of making |
US20090056120A1 (en) * | 1999-10-04 | 2009-03-05 | Bhullar Raghbir S | Biosensor and method of making |
WO2001057510A2 (en) | 2000-02-02 | 2001-08-09 | Lifescan, Inc. | Electrochemical methods and devices for use in the determination of hematocrit corrected analyte concentrations |
KR20030036804A (ko) | 2000-09-20 | 2003-05-09 | 가부시키가이샤 미쿠니 | 연료공급장치 및 이 연료공급장치에 사용되는 연료필터 |
JP2006504096A (ja) * | 2002-10-23 | 2006-02-02 | アボット・ラボラトリーズ | ヘマトクリット及び酸素バイアスを有するバイオセンサ |
KR100586832B1 (ko) * | 2004-08-27 | 2006-06-08 | 주식회사 인포피아 | 바이오센서의 시료반응결과 측정장치 |
KR20050010720A (ko) | 2004-09-24 | 2005-01-28 | 한상배 | 자흡기능과 교반기능이 구비된 유체공급장치와 이를이용한 포기장치 |
KR20070021086A (ko) | 2005-08-17 | 2007-02-22 | 가부시키가이샤 고베 세이코쇼 | 소스/드레인 전극, 박막 트랜지스터 기판, 그의 제조방법,및 표시 디바이스 |
KR20070025106A (ko) | 2005-08-31 | 2007-03-08 | 엘지전자 주식회사 | 멀티형 공기조화기의 압축기 오일 회수장치 |
KR20070030346A (ko) | 2005-09-13 | 2007-03-16 | 전달용 | 수익 자동 배분 방법 및 시스템 |
US20100219084A1 (en) * | 2006-10-05 | 2010-09-02 | Stephen Patrick Blythe | Method for determining hematocrit corrected analyte concentrations |
KR20070020447A (ko) | 2006-11-07 | 2007-02-21 | 지. 톰프슨 로저 | 즉각적으로 착용할 수 있는 표식을 가진 모자 챙 성형 장치 |
US20110139634A1 (en) | 2009-12-14 | 2011-06-16 | Taidoc Technology Corporation | System and method for measuring analyte concentration with interferant correction |
Non-Patent Citations (3)
Title |
---|
E. K. BAUMAN ET AL., ANALYTICAL CHEMISTRY, vol. 37, 1965, pages 1378 |
K. B. OLDHAM: "Microelectrodes: Theory and Applications", 1991, KLUWER ACADEMIC PUBLISHERS |
See also references of EP2871470A4 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105510391A (zh) * | 2014-09-22 | 2016-04-20 | 英科新创(厦门)科技有限公司 | 一种电极式血糖试条 |
WO2016145721A1 (zh) * | 2015-03-14 | 2016-09-22 | 深圳市贝沃德克生物技术研究院有限公司 | 用于血糖仪试纸针的试纸条 |
Also Published As
Publication number | Publication date |
---|---|
JP2015518161A (ja) | 2015-06-25 |
CN104380095B (zh) | 2016-08-24 |
US9753004B2 (en) | 2017-09-05 |
CN104380095A (zh) | 2015-02-25 |
KR101466222B1 (ko) | 2014-12-01 |
EP2871470A1 (en) | 2015-05-13 |
EP2871470B1 (en) | 2016-07-20 |
EP2871470A4 (en) | 2015-06-03 |
US20150083613A1 (en) | 2015-03-26 |
JP5873215B2 (ja) | 2016-03-01 |
KR20130135605A (ko) | 2013-12-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2013180528A1 (ko) | 정확도가 향상된 전기화학적 바이오센서 | |
US11162916B2 (en) | Accurate analyte measurements for electrochemical test strip based on sensed physical characteristic(s) of the sample containing the analyte | |
JP4018082B2 (ja) | 電気化学バイオセンサ | |
JP4418435B2 (ja) | 電気化学的バイオセンサー | |
JP3948627B2 (ja) | 電気化学的バイオセンサー | |
AU2011303639B2 (en) | Analyte measurement method and system with hematocrit compensation | |
JP4927996B2 (ja) | 一定少量の試料を均一に導入可能な試料導入チャンネルを具備した電気化学的バイオセンサ | |
US10024816B2 (en) | Personal blood glucose meter and abnormal measurement detection method using same | |
WO2016046343A1 (en) | Accurate analyte measurements for electrochemical test strip to determine analyte measurement time based on measured temperature, physical characteristic and estimated analyte value and their temperature compensated values | |
JP2010054379A (ja) | バイオセンサ、その製造方法、その使用方法 | |
JP5509969B2 (ja) | 測定装置及び測定方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 13796996 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2015514916 Country of ref document: JP Kind code of ref document: A |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REEP | Request for entry into the european phase |
Ref document number: 2013796996 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2013796996 Country of ref document: EP |