WO2013166338A3 - Système de capture cellulaire et son utilisation - Google Patents

Système de capture cellulaire et son utilisation Download PDF

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Publication number
WO2013166338A3
WO2013166338A3 PCT/US2013/039350 US2013039350W WO2013166338A3 WO 2013166338 A3 WO2013166338 A3 WO 2013166338A3 US 2013039350 W US2013039350 W US 2013039350W WO 2013166338 A3 WO2013166338 A3 WO 2013166338A3
Authority
WO
WIPO (PCT)
Prior art keywords
capture system
cell capture
cells
cell
determining
Prior art date
Application number
PCT/US2013/039350
Other languages
English (en)
Other versions
WO2013166338A2 (fr
Inventor
Norman R. Wainwright
Bala S. Manian
Eric STIMPSON
Brian J. KOLONIA
Robert K. KOLONIA
Original Assignee
Charles River Laboratories, Inc.
Reametrix Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Charles River Laboratories, Inc., Reametrix Inc. filed Critical Charles River Laboratories, Inc.
Priority to CN201380035711.6A priority Critical patent/CN104685346B/zh
Priority to JP2015510466A priority patent/JP6240162B2/ja
Priority to EP13730385.5A priority patent/EP2769204B1/fr
Publication of WO2013166338A2 publication Critical patent/WO2013166338A2/fr
Publication of WO2013166338A3 publication Critical patent/WO2013166338A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Optics & Photonics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un système de capture cellulaire destiné à être utilisé dans la détermination de la présente et/ou de la quantité de cellules, par exemple, de cellules viables, dans un échantillon liquide, et des procédés d'utilisation d'un tel système de capture cellulaire.
PCT/US2013/039350 2012-05-02 2013-05-02 Système de capture cellulaire et son utilisation WO2013166338A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201380035711.6A CN104685346B (zh) 2012-05-02 2013-05-02 细胞捕获系统及其使用
JP2015510466A JP6240162B2 (ja) 2012-05-02 2013-05-02 細胞収集システムおよびその使用
EP13730385.5A EP2769204B1 (fr) 2012-05-02 2013-05-02 Système de capture cellulaire et son utilisation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261641812P 2012-05-02 2012-05-02
US61/641,812 2012-05-02
US201361784807P 2013-03-14 2013-03-14
US61/784,807 2013-03-14

Publications (2)

Publication Number Publication Date
WO2013166338A2 WO2013166338A2 (fr) 2013-11-07
WO2013166338A3 true WO2013166338A3 (fr) 2014-01-09

Family

ID=48670054

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/039350 WO2013166338A2 (fr) 2012-05-02 2013-05-02 Système de capture cellulaire et son utilisation

Country Status (5)

Country Link
US (2) US10324036B2 (fr)
EP (1) EP2769204B1 (fr)
JP (1) JP6240162B2 (fr)
CN (1) CN104685346B (fr)
WO (1) WO2013166338A2 (fr)

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US10324036B2 (en) 2012-05-02 2019-06-18 Charles River Laboratories, Inc. Porous planar cell capture system
CN104755931B (zh) 2012-05-02 2016-08-31 查尔斯河实验室公司 用于检测细胞样品中的活细胞的方法
EP2769218B1 (fr) 2012-05-02 2016-08-10 Charles River Laboratories, Inc. Procédé de coloration liée à la viabilité
CN105874052B (zh) 2013-11-04 2019-05-21 查尔斯河实验室公司 过滤系统和其使用
US20160298162A1 (en) * 2013-11-04 2016-10-13 Charles River Laboratories, Inc. Method for detecting viable cells in a cell sample
US9436866B2 (en) 2014-08-01 2016-09-06 General Electric Company High sensitivity flat panel microbiology detection and enumeration system
CA3014099A1 (fr) * 2016-02-18 2017-08-24 Optofluidics, Inc. Systeme et procede de caracterisation de particules dans un echantillon de fluide
US11035795B2 (en) 2016-02-18 2021-06-15 Optofluidics, Inc. Methods for identification of particles in a fluid sample
GB201716883D0 (en) * 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
US11609224B2 (en) 2017-10-26 2023-03-21 Essenlix Corporation Devices and methods for white blood cell analyses
WO2019210025A1 (fr) * 2018-04-25 2019-10-31 Optofluidics Inc. Collecteur à vide pour microscopie à filtration
EP3837543A4 (fr) * 2018-08-16 2022-11-16 Essenlix Corporation Analyse cellulaire dans des fluides corporels, notamment le sang
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JP2015521038A (ja) 2015-07-27
US10324036B2 (en) 2019-06-18
US20190187056A1 (en) 2019-06-20
US10976258B2 (en) 2021-04-13
US20130316363A1 (en) 2013-11-28
JP6240162B2 (ja) 2017-11-29
EP2769204A2 (fr) 2014-08-27

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