Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
  • G01N1/31—Apparatus therefor
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
  • B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
  • B05C5/00—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work
  • B05C5/02—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work the liquid or other fluent material being discharged through an outlet orifice by pressure, e.g. from an outlet device in contact or almost in contact, with the work
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
  • B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
  • B05D1/00—Processes for applying liquids or other fluent materials
  • B05D1/40—Distributing applied liquids or other fluent materials by members moving relatively to surface
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
  • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Definitions

• a common approach for the separation of the target cells from other cells is by immunomagnetic methods with the use of an antibody towards an antigen which is specific for the target cells.
• This antibody is coupled to magnetic particles which can then in turn be used to isolate the target from the sample by means of a magnetic field.
• the technology is used in a multitude of commercial and non-commercial systems.
• Other approaches use the concept of size exclusion by filtration of sample material (for example ScreenCell or On-Q- ity).
• cells can be immobilized on surfaces and then identified as positive cells by immunocytochemistry and/or morphological analysis (e.g. Epic Science).
• these approaches are limited to cell suspensions of low volume.
• non-target cells usually white blood cells with a diameter of 5-7 ⁇
• target cells of interest for example circulating tumour cells with a diameter of >10 ⁇
• target cells which display no size differences to non-target cells might not be isolated by these methods.
• At least some embodiments include a method that allows the uniform distribution of cell components suspended in a liquid (cells or components thereof) on a surface.
• a uniform distribution of the liquid and of any material suspended in the liquid, and any substances or particles suspended in the liquid on the surface of a carrier object is available.
• This material can in particular comprise living or fixed cells (for example of human, animal or plant origin), bacteria, viruses, cell components, proteins, nucleic acids, metabolites or chemicals.
• the distributing bar 33 is mounted freely swivelling on a support structure 34 with a single support, which can be moved in the plane of the liquid carrier 32 in two directions of motion 43 and 44 mutually independently, as is shown by arrows.
• the distributing bar 33 can be tracked away from the table 31 in one of the directions of motion 43 and 44 shown, so that access to the liquid carrier upper surface 32a is possible without hindrance.

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Immunology (AREA)
• Pathology (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Physics & Mathematics (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Biomedical Technology (AREA)
• Molecular Biology (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Sampling And Sample Adjustment (AREA)
• Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
• Application Of Or Painting With Fluid Materials (AREA)
• Investigating Or Analysing Biological Materials (AREA)

Priority Applications (4)

Applications Claiming Priority (2)

Related Child Applications (1)

Publications (1)

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Family Applications (1)

Country Status (5)

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Citations (4)

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• 2013
  • 2013-04-05 WO PCT/US2013/035520 patent/WO2013152330A1/en not_active Ceased
  • 2013-04-05 CA CA2869615A patent/CA2869615A1/en not_active Abandoned
  • 2013-04-05 AU AU2013243322A patent/AU2013243322A1/en not_active Abandoned
  • 2013-04-05 JP JP2015504759A patent/JP6272296B2/ja active Active
• 2014
  • 2014-10-01 US US14/503,473 patent/US20150024429A1/en not_active Abandoned

Patent Citations (4)

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