JP6272296B2 - 懸濁された細胞成分を均質に分布させるための方法と装置 - Google Patents
懸濁された細胞成分を均質に分布させるための方法と装置 Download PDFInfo
- Publication number
- JP6272296B2 JP6272296B2 JP2015504759A JP2015504759A JP6272296B2 JP 6272296 B2 JP6272296 B2 JP 6272296B2 JP 2015504759 A JP2015504759 A JP 2015504759A JP 2015504759 A JP2015504759 A JP 2015504759A JP 6272296 B2 JP6272296 B2 JP 6272296B2
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- liquid carrier
- distribution bar
- cell
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009826 distribution Methods 0.000 title claims description 99
- 230000001413 cellular effect Effects 0.000 title claims description 55
- 238000000034 method Methods 0.000 title claims description 48
- 239000007788 liquid Substances 0.000 claims description 224
- 210000004027 cell Anatomy 0.000 claims description 101
- 210000003850 cellular structure Anatomy 0.000 claims description 29
- 238000001035 drying Methods 0.000 claims description 9
- 239000011248 coating agent Substances 0.000 claims description 7
- 238000000576 coating method Methods 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims description 5
- 230000000717 retained effect Effects 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 3
- 238000009595 pap smear Methods 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims 1
- 230000033001 locomotion Effects 0.000 description 20
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 239000000969 carrier Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 239000002245 particle Substances 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 238000009827 uniform distribution Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 239000010410 layer Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 230000002380 cytological effect Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 description 2
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 230000006727 cell loss Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 230000002489 hematologic effect Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 150000002634 lipophilic molecules Chemical class 0.000 description 2
- 239000006247 magnetic powder Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 241000203069 Archaea Species 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000037323 Rare tumor Diseases 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 210000003793 centrosome Anatomy 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000003040 circulating cell Anatomy 0.000 description 1
- 210000005266 circulating tumour cell Anatomy 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- -1 polyethylene terephthalate Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920005553 polystyrene-acrylate Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000011120 smear test Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
- 229910052726 zirconium Inorganic materials 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05C5/00—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work
- B05C5/02—Apparatus in which liquid or other fluent material is projected, poured or allowed to flow on to the surface of the work the liquid or other fluent material being discharged through an outlet orifice by pressure, e.g. from an outlet device in contact or almost in contact, with the work
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D1/00—Processes for applying liquids or other fluent materials
- B05D1/40—Distributing applied liquids or other fluent materials by members moving relatively to surface
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Application Of Or Painting With Fluid Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261621107P | 2012-04-06 | 2012-04-06 | |
| US61/621,107 | 2012-04-06 | ||
| PCT/US2013/035520 WO2013152330A1 (en) | 2012-04-06 | 2013-04-05 | Method and device for the homogeneous distribution of suspended cell components |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015519041A JP2015519041A (ja) | 2015-07-09 |
| JP2015519041A5 JP2015519041A5 (enExample) | 2017-01-26 |
| JP6272296B2 true JP6272296B2 (ja) | 2018-01-31 |
Family
ID=48191024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015504759A Active JP6272296B2 (ja) | 2012-04-06 | 2013-04-05 | 懸濁された細胞成分を均質に分布させるための方法と装置 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20150024429A1 (enExample) |
| JP (1) | JP6272296B2 (enExample) |
| AU (1) | AU2013243322A1 (enExample) |
| CA (1) | CA2869615A1 (enExample) |
| WO (1) | WO2013152330A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10336461B2 (en) * | 2016-01-05 | 2019-07-02 | The Boeing Company | Aircraft engine and associated method for driving the fan with the low pressure shaft during taxi operations |
| US10584694B2 (en) * | 2016-02-09 | 2020-03-10 | Oridion Medical 1987 Ltd. | Miniature diaphragm pump with enlarged operation time |
| JP6878163B2 (ja) * | 2017-06-15 | 2021-05-26 | 株式会社京都製作所 | 培養装置および培養方法 |
| CN113125214B (zh) * | 2019-12-31 | 2025-08-15 | 苏州图灵微生物科技有限公司 | 一种制样装置 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4359013A (en) * | 1980-09-08 | 1982-11-16 | Prevo Donald L | Device for spreading monolayered films |
| CA1164647A (en) * | 1980-09-08 | 1984-04-03 | Donald L. Prevo | Device for spreading monolayered films |
| IL74774A (en) * | 1984-04-23 | 1988-05-31 | Abbott Lab | Method for the preparation of immunocytochemical slides with a polylysine solution |
| JPH11337462A (ja) * | 1998-05-25 | 1999-12-10 | Nippon Koatec:Kk | 塗抹標本作成装置 |
| US6180314B1 (en) * | 1998-05-27 | 2001-01-30 | Becton, Dickinson And Company | Method for preparing thin liquid samples for microscopic analysis |
| FR2782800B1 (fr) * | 1998-09-01 | 2000-10-20 | Abx Sa | Dispositif pour la preparation automatique d'etalements sanguins sur des lames |
| US20020042081A1 (en) * | 2000-10-10 | 2002-04-11 | Eric Henderson | Evaluating binding affinities by force stratification and force panning |
| JP2007120969A (ja) * | 2005-10-25 | 2007-05-17 | Yoshikawa Kogyo Co Ltd | 塗抹標本作製装置 |
| US20090176312A1 (en) * | 2007-12-04 | 2009-07-09 | Selinfreund Richard H | Biological and chemical test media and system |
| BR112012011181A2 (pt) * | 2009-11-13 | 2020-10-13 | Ventana Medical Systems, Inc. | ''estação de processsamento de peças corrediça automatizada e método de processamento de uma amostra'' |
| WO2011139978A1 (en) * | 2010-05-04 | 2011-11-10 | Ventana Medical Systems, Inc. | Moving meniscus rinsing and mixing in cell staining |
-
2013
- 2013-04-05 WO PCT/US2013/035520 patent/WO2013152330A1/en not_active Ceased
- 2013-04-05 CA CA2869615A patent/CA2869615A1/en not_active Abandoned
- 2013-04-05 AU AU2013243322A patent/AU2013243322A1/en not_active Abandoned
- 2013-04-05 JP JP2015504759A patent/JP6272296B2/ja active Active
-
2014
- 2014-10-01 US US14/503,473 patent/US20150024429A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013152330A1 (en) | 2013-10-10 |
| CA2869615A1 (en) | 2013-10-10 |
| US20150024429A1 (en) | 2015-01-22 |
| AU2013243322A1 (en) | 2014-10-23 |
| JP2015519041A (ja) | 2015-07-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Monguió‐Tortajada et al. | Extracellular‐vesicle isolation from different biological fluids by size‐exclusion chromatography | |
| US9290812B2 (en) | Methods and compositions for separating rare cells from fluid samples | |
| EP1694856B1 (en) | Filtration unit and method | |
| US8969021B2 (en) | Methods and compositions for detecting non-hematopoietic cells from a blood sample | |
| US6949355B2 (en) | Methods, compositions, and automated systems for separating rare cells from fluid samples | |
| US9568403B2 (en) | Particle processing | |
| Marqués-García et al. | Protocols for exosome isolation and RNA profiling | |
| KR20010023340A (ko) | 유체 시료로부터 분리된 입자성 물질의 단층을자동형성하기 위한 방법 및 장치 | |
| JP6272296B2 (ja) | 懸濁された細胞成分を均質に分布させるための方法と装置 | |
| WO2016005741A1 (en) | Cell positioning and analysis device | |
| Fisher et al. | Cell separation: a practical approach | |
| CN109153882B (zh) | 细胞外小泡回收方法及细胞外小泡用容器 | |
| Swennenhuis et al. | Sample preparation methods following cellsearch approach compatible of single-cell whole-genome amplification: an overview | |
| JP4679847B2 (ja) | 細胞の解析方法 | |
| US20030044766A1 (en) | Methods and devices for detecting cell-cell interactions | |
| US20110300618A1 (en) | Semi-automated device for single parameter and multi-parameter phenotyping of cells | |
| Lee et al. | Enrichment of circulating tumor cells using a centrifugal affinity plate system | |
| JP2018157812A (ja) | 試料中に含まれる細胞の検出方法 | |
| WO2022082025A1 (en) | Cellular response analysis method | |
| UA76372C2 (en) | Method for producing cytological preparation of dendritic cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160208 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20160831 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160906 |
|
| A524 | Written submission of copy of amendment under article 19 pct |
Free format text: JAPANESE INTERMEDIATE CODE: A524 Effective date: 20161205 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20170530 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20171002 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20171010 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20171205 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20171228 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6272296 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |