WO2013128772A1 - Dispositif de test par électrophorèse par électrofocalisation et son procédé de production - Google Patents

Dispositif de test par électrophorèse par électrofocalisation et son procédé de production Download PDF

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Publication number
WO2013128772A1
WO2013128772A1 PCT/JP2012/083782 JP2012083782W WO2013128772A1 WO 2013128772 A1 WO2013128772 A1 WO 2013128772A1 JP 2012083782 W JP2012083782 W JP 2012083782W WO 2013128772 A1 WO2013128772 A1 WO 2013128772A1
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Prior art keywords
gel layer
gel
test device
outer peripheral
isoelectric focusing
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PCT/JP2012/083782
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English (en)
Japanese (ja)
Inventor
政俊 中川
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シャープ株式会社
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Publication of WO2013128772A1 publication Critical patent/WO2013128772A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Definitions

  • the present invention relates to a test device for isoelectric focusing and a method for producing the same.
  • Electrophoresis is a separation analysis method using a phenomenon in which a charged substance in a medium moves in an electric field according to the electric charge when a voltage is applied to the medium such as a solution or a hydrophilic support immersed in the solution. It is.
  • electrophoresis using gel as a medium is a technique for separating biopolymers such as proteins and nucleic acids, in bioscience, molecular biology and other life science fields and clinical laboratory fields. Widely used.
  • electrophoresis isoelectric focusing method
  • proteins are separated by gathering at a pH position equal to their isoelectric point in a pH gradient.
  • an amphoteric carrier has been used in the past, but in recent years, an immobilized pH gradient (Immobilized pH Gradient: IPG) gel that does not collapse during energization is often used. ing.
  • IPG immobilized pH Gradient
  • gel electrophoresis is an indispensable technique for separating and analyzing biopolymers such as proteins.
  • the accuracy and reproducibility of analysis largely depend on the quality of the gel used. Therefore, in this field, it is desired to develop a technique capable of stably producing an electrophoretic test device equipped with a high-resolution gel.
  • Patent Document 1 discloses a gel sheet having a concentration gradient by mixing two types of gel stock solutions having different concentrations in a stirring tank, and introducing the mixed solution into a gel container from the bottom to cause gelation (polymerization).
  • a method of making is disclosed.
  • an SDS-PAGE gel sheet having a predetermined concentration gradient can be obtained by changing the ratio of each gel stock solution in the mixed solution to be introduced into the gel container.
  • two types of gel stock solutions having different pH are mixed in a stirring tank, and the mixture is introduced into the gel container from the bottom to cause gelation, thereby adjusting the pH gradient.
  • the gel sheet which has can be produced.
  • a gel sheet having a predetermined pH gradient is obtained by changing the ratio of each gel stock solution in the mixed solution to be introduced into the gel container, and the gel sheet is elongated by cutting it at a predetermined width in the pH gradient direction.
  • a gel plate for isoelectric focusing is obtained.
  • Patent Document 2 discloses a gel plate manufacturing method in which a monomer solution is applied onto a plate as a technique capable of accurately managing a concentration gradient or pH gradient. That is, after forming a puddle on the plate and discharging a monomer solution into the puddle, a polymerization initiator is applied to gel the coating film, thereby forming a gel layer on the substrate.
  • an SDS-PAGE gel plate having a predetermined concentration gradient or a predetermined pH gradient is prepared by mixing two types of monomer solutions having different concentrations or pHs and applying them to the pool while changing the mixing ratio.
  • a gel plate for isoelectric focusing is obtained.
  • the gel layer In order to be able to store the gel layer produced as described in Patent Documents 1 and 2 for a long period of time, the gel layer is usually dried. And a gel layer is decompress
  • the gel layer G 11 for isoelectric focusing of Patent Document 1 As shown in FIG. 8 (A), the gel layer G 11 swollen by absorbing water into the dry film is formed on the plate P 11 . It swells by protruding beyond the outer peripheral end face (end face in four directions). Therefore, as shown in FIG. 8 (B), the gel layer G 11 of the first gel plate GP 11 used for the first-dimensional electrophoresis (isoelectric focusing) in the two-dimensional electrophoresis is used in the two-dimensional electrophoresis.
  • the protein separated by the gel layer G 11 of the first gel plate GP 11 is pressed onto the gel layer G 12 of the second gel plate GP 12 for second-dimensional electrophoresis (SDS-PAGE), and the second gel plate GP.
  • SDS-PAGE second-dimensional electrophoresis
  • the protein present in the gel layer G 11 of the protruding portion (shaded portion) of the first gel plate GP 11 does not move sufficiently or not to the gel layer G 12 of the second gel plate GP 12 .
  • the gel layer G 21 does not contact the gel layer G 22 of the second gel plate GP 22 sufficiently or not.
  • proteins present in the gel layer G21 of the outer peripheral portion of the first gel plate G21 (hatched portion) is not enough or no movement in the gel layer G 22 of the second gel plate GP 22.
  • the present invention has been made in view of such problems, and an object thereof is to provide an isoelectric focusing test device capable of performing highly reliable two-dimensional electrophoresis and a method for manufacturing the same. .
  • an isoelectric focusing test device in which a dry film obtained by drying a gel layer is formed on a substrate, and when the dry film is swollen (1) gel The layer has an outer peripheral end surface perpendicular to the substrate; (2) The outer peripheral end surface of the gel layer and the outer peripheral end surface of the substrate are on the same plane, (3) The film thickness of the outer peripheral part in the gel layer is the thickest and the film thickness of the central part is the thinnest, (4) An isoelectric focusing test device that provides a gel layer satisfying the conditions (1) to (4) that the thickness of the central portion of the gel layer is 80 to 90% of the thickness of the outer peripheral portion. Provided.
  • the said frame is removed, and the said gel layer is removed.
  • the frame is provided with a method for producing a test device for isoelectric focusing, which has a hydrophilically treated inner peripheral surface in contact with the gel material liquid.
  • the dry film of the isoelectric focusing test device absorbs water and swells, so that it does not have a portion protruding from the outer peripheral end surface of the substrate, and the center portion and the outer peripheral end portion A gel layer having a small film thickness difference can be restored. Therefore, after performing the first-dimensional electrophoresis in the two-dimensional electrophoresis using the isoelectric focusing test device of the present invention, this test device (hereinafter sometimes referred to as “first-dimensional gel plate”) When the gel layer is pressed against the gel layer of the second-dimensional electrophoresis test device (hereinafter sometimes referred to as “second-dimensional gel plate”), the gel layer of the first-dimensional gel plate becomes the gel layer of the second-dimensional gel plate.
  • first-dimensional gel plate When the gel layer is pressed against the gel layer of the second-dimensional electrophoresis test device (hereinafter sometimes referred to as “second-dimensional gel plate”), the gel layer of the first-dimensional gel plate becomes the gel layer of the second-dimensional gel plate
  • the separated protein existing in the gel layer of the first dimension gel plate can be accurately and sufficiently moved (transferred) to the gel layer of the second dimension gel plate, and the reliability of the second dimension electrophoresis High analysis results can be obtained.
  • FIG. 3 is a partially omitted cross-sectional view showing a state in which the isoelectric focusing test device of Embodiment 1 is overlaid on the second-dimensional electrophoresis testing device.
  • FIG. 3 is a partially omitted cross-sectional view showing a state in which the isoelectric focusing test device is pressed from the state of FIG.
  • FIG. 9 is a partially omitted cross-sectional view illustrating a state in which the isoelectric focusing test device is pressed from the state of FIG.
  • FIG. 5 is a partially omitted cross-sectional view showing a state in which another conventional isoelectric focusing test device is stacked on a second-dimensional electrophoresis test device.
  • FIG. 10 is a partially omitted cross-sectional view illustrating a state in which the isoelectric focusing test device is pressed from the state of FIG.
  • the isoelectric focusing test device of the present invention is an isoelectric focusing test device in which a dry film obtained by drying a gel layer is formed on a substrate, and the dry film is swollen.
  • the gel layer has an outer peripheral end surface in a direction perpendicular to the base material, (2) The outer peripheral end surface of the gel layer and the outer peripheral end surface of the substrate are on the same plane, (3) The film thickness of the outer peripheral part in the gel layer is the thickest and the film thickness of the central part is the thinnest, (4) The gel layer satisfies the conditions (1) to (4) that the thickness of the central portion of the gel layer is 80 to 90% of the thickness of the outer peripheral portion.
  • the gel layer satisfying the conditions (1) to (4) refers to a gel layer in a water saturated state.
  • the isoelectric focusing test device of the present invention is such that a dry film obtained by drying a gel layer satisfying the above conditions (1) to (4) is formed on a substrate.
  • the surface of the gel layer opposite to the base material is formed into a gently convex surface without a raised portion. It is preferable. That is, when the gel layer of the first-dimensional gel plate is superimposed on the gel layer of the second-dimensional gel plate and pressed, the surface of the gel layer opposite to the substrate becomes a pressing surface, and this pressing surface is a gentle concave. When it is a curved surface, the air between the upper and lower gel layers is easily pushed out to the outer peripheral side, and the adhesion between the upper and lower gel layers is further increased.
  • the separated protein existing in the gel layer of the first dimension gel plate can be surely moved to the gel layer of the second dimension gel plate, which is more reliable and more accurate for the second dimension electrophoresis. Analysis results can be obtained.
  • the “gradual concave surface” includes a case where the lowest part is a flat surface.
  • the softness of the gel layer is not particularly limited, but considering the case where the isoelectric focusing test device of the present invention is used as the first-dimensional gel plate in two-dimensional electrophoresis, the first-dimensional gel plate
  • the gel layer is preferably softer than the gel layer of the second-dimensional gel plate.
  • the length and width of the gel layer are the same as the length and width of the substrate.
  • the length and width of the substrate are not particularly limited, but as an example, the length is about 50 to 250 mm, and the width is about 0.5 to 5 mm.
  • the film thickness of the gel layer is not particularly limited.
  • the film thickness of the thickest outer peripheral edge is about 200 to 1000 ⁇ m, and the film thickness of the thinnest central part is 80 to 90%.
  • the thickness of the dried film obtained by drying the gel layer shrinks to 100 ⁇ m or less, but the length and width hardly change.
  • the form of the base material of the electrophoresis test device is not particularly limited, and examples thereof include an elongated plate and a chip molded into a predetermined shape.
  • the material of the base material is not particularly limited as long as it can function as a base material for a test device for electrophoresis.
  • glass such as quartz glass and non-alkali glass, polyethylene terephthalate (PET), polymethacryl
  • resins such as acid methyl resin (PMMA), ceramics such as alumina, and low-temperature co-fired ceramic.
  • the surface of the substrate on which the gel layer is formed may be subjected to a hydrophilic treatment, thereby improving the wettability of the monomer solution described below with respect to the substrate, and the monomer solution Adhesion between the gelled gel layer and the substrate is improved.
  • a hydrophilic treatment include nitration using sulfuric acid, sulfonation using nitric acid, oxygen plasma treatment and the like.
  • the material of the gel layer of the electrophoresis test device is not particularly limited as long as it can function as the gel layer of the electrophoresis test device.
  • acrylamide Monomer
  • bisacrylamide crosslinking agent
  • pH adjusting material pH buffer
  • TEMED polymerization accelerator
  • ammonium persulfate APS
  • the gel layer is formed by applying a gel material solution on a base material to cause gelation.
  • a gel material solution to which a polymerization initiator has been added in advance (a gel material solution containing a polymerization initiator) is applied on the substrate, a monomer solution in which materials other than the polymerization initiator are mixed (not including a polymerization initiator) After the gel material liquid) is applied onto the substrate, a polymerization initiator may be applied onto the coating film, and the present invention includes both cases.
  • gel material liquid means all of a gel material liquid to which a polymerization initiator has been added in advance, a gel material liquid not containing a polymerization initiator, and a polymerization initiator, unless otherwise specified.
  • a gel material solution to which a polymerization initiator has been added in advance may be referred to as “a gel material solution containing a polymerization initiator”
  • a gel material solution that does not include a polymerization initiator may be referred to as a “monomer solution”.
  • the test device for isoelectric focusing of the present invention includes a step of applying a gel material solution on a base material surrounded by a frame to form a gel layer, and removing the frame to form the gel layer.
  • the frame body can be manufactured by a manufacturing method having a hydrophilically treated inner peripheral surface in contact with the gel material liquid.
  • the method for applying the gel material liquid onto the substrate is not particularly limited, and any method can be used as long as the gel material liquid can be applied to a predetermined region on the upper surface of the substrate.
  • a pipetter, a dispenser, an inkjet device, etc. Can be mentioned.
  • FIG. 1 (A) is a perspective view showing a usable state of the isoelectric focusing test device of Embodiment 1 of the present invention
  • FIG. 1 (B) is the isoelectric focusing of FIG. 1 (A). It is a perspective view which shows the state which can be preserve
  • the isoelectric focusing test device GP 1 shown in FIG. 1A is obtained by forming a gel layer G 1 satisfying the following conditions (1) to (4) on a substrate S.
  • the gel layer G 1 has an outer peripheral end face G 1a perpendicular to the substrate S.
  • the outer peripheral end face G 1a of the gel layer G 1 and the outer peripheral end face Sa of the substrate S are on the same plane.
  • the film thickness T 1 at the outer peripheral edge of the gel layer G 1 is the thickest and the film thickness T 2 at the center is the thinnest.
  • the film thickness T 2 at the center of the gel layer G 1 is 80 to 90% of the film thickness T 1 at the outer peripheral edge.
  • the above conditions (1) to (4) are obtained by causing water to be absorbed and swelled in the dry film D 1 of the storable isoelectric focusing test instrument GPD 1 in FIG. 1 (B).
  • the gel layer G 1 (see FIG. 1A) satisfying (1) is restored.
  • This gel layer G 1 has a gently concave curved upper surface (surface opposite to the substrate) and an outer peripheral end surface G 1a perpendicular to the surface of the substrate S in contact with the gel layer G 1.
  • the outer peripheral end face G 1a of the gel layer G 1 is on the same plane as the outer peripheral end face Sa of the substrate S.
  • the gel layer G 1 satisfying these conditions (1) to (4) does not have a portion protruding from the outer peripheral end surface Sa of the substrate S, and the film thickness difference (T 1 ⁇ between the outer peripheral end portion and the central portion).
  • T 2 is a small gel layer. Therefore, when the isoelectric focusing test device GP 1 is used as a first-dimensional gel plate in two-dimensional electrophoresis, the analysis result of the first-dimensional electrophoresis can be accurately reflected in the second-dimensional electrophoresis. .
  • the concave curved surface G 1b of the gel layer G 1 of the first-dimensional gel plate GP 1 is superimposed on the upper surface of the gel layer G 22 of the second-dimensional gel plate GP 22 .
  • the vicinity of the central portion of the concave curved surface G 1b in the upper gel layer G 1 may not be in contact with the upper surface of the lower gel layer G 22 .
  • the entire surface of the first dimension gel plate GP 1 gel layer G 1 of the concave curved surface G 1b is the second dimension It contacts the upper surface of the gel layer G 22 of the gel plate GP 22 .
  • the separated proteins existing in the gel layer G 1 of the first-dimensional gel plate GP 1 can be accurately and sufficiently moved to the gel layer G 22 of the second-dimensional gel plate GP 22 , Analytical results with high migration reliability can be obtained.
  • the isoelectric focusing test device GPD 1 shown in FIG. can get.
  • FIG. 3 is a configuration diagram showing an apparatus capable of manufacturing the electrophoresis test device of the first embodiment.
  • This test device manufacturing apparatus moves a stage 10, a tray 11 installed on the stage 10, a frame 12 installed on the tray 11, an inkjet device 30 as an application unit, and the stage 10 in a linear direction.
  • the case 50 is provided with an opening / closing door (not shown).
  • the frame 11 is set on the stage 10, and the base material S is set in the frame 12.
  • the frame body 11 and the stage 10 can be formed of the same material as the base material S.
  • Frame 11 has a recess 11a of the same size and shape as the substrate S, the inner peripheral surface 11a 1 of the concave portion 11a is processed hydrophilic.
  • the hydrophilic treatment include a method of forming a film by a predetermined method such as dip coating with a commercially available hydrophilic functional coating.
  • the moving mechanism 40 includes a support base 40a that supports the stage 10, and the support base 40a can be reciprocated in a linear direction by a linear guide mechanism (not shown).
  • the support base 40a indicated by the solid line is in the standby position, and the support base 40a, the stage 10, the tray 11, the frame body 12, and the base material S go straight to the position indicated by the two-dot chain line in the coating process. .
  • the substrate S set on the tray 11 passes directly below first to third inkjet heads 31b, 32b, and 33b, which will be described later.
  • the ink jet device 30 includes an acidic solution discharge unit 31, a basic solution discharge unit 32, a polymerization initiator discharge unit 33, and a negative pressure adjustment unit 34.
  • the acidic solution discharge unit 31 includes a first tank 31a that stores the acidic monomer solution A, a first inkjet head 31b, and a first pipe 31c that sends the acidic monomer solution A from the first tank 31a to the first inkjet head 31b. And the acidic monomer solution A is supplied from the first tank 31a to the first inkjet head 31b using the water head difference.
  • the basic solution discharge unit 32 includes a second tank 32a that stores the basic solution B, a second inkjet head 32b, and a second pipe 32c that sends the basic solution B from the second tank 32a to the second inkjet head 32b. And the basic monomer solution B is supplied from the second tank 32a to the second inkjet head 32b using the water head difference.
  • the polymerization initiator discharge unit 33 includes a third tank 33a that stores the polymerization initiator C, a third inkjet head 33b, and a third pipe 33c that sends the polymerization initiator C from the third tank 33a to the third inkjet head 33b. And the polymerization initiator C is supplied from the third tank 33a to the third inkjet head 33b using the water head difference.
  • Examples of the first to third ink jet heads 31b to 33b include a thermal jet method, a piezo jet method, an electrostatic drive method, and the like, but each liquid (acidic monomer solution A, basic monomer solution B, polymerization in the ink jet device 30).
  • a thermal jet method a piezo jet method, an electrostatic drive method, and the like
  • each liquid acidic monomer solution A, basic monomer solution B, polymerization in the ink jet device 30.
  • the initiator C is cooled, it is desirable to use a piezo jet method or an electrostatic drive method without using a thermal jet method for applying heat to each liquid.
  • the negative pressure adjusting unit 34 is connected to the first to third tanks 31a to 33a by pipes 35 to 37, manages the atmospheric pressure in the first to third tanks, and controls the first to third inkjet heads 31b.
  • the insides of the first to third tanks 31a to 33a are adjusted to be constant at a predetermined pressure lower than the atmospheric pressure so that the liquid does not drip from the nozzle holes H (see FIG. 5).
  • the first to third ink jet heads 31b to 33b are integrated to form a set of discharge head units U, which are fixed by a fixing member (not shown). As shown in FIG. 4, the first to third ink jet heads 31b to 33b are arranged in a line on the movement locus E of the base material S, but the head arrangement order is not limited to this order. In the first embodiment, the first to third inkjet heads 31b to 33b are arranged in this order from the upstream side in the moving direction of the substrate S.
  • a plurality of nozzles are formed on the lower surfaces of the first to third inkjet heads 31b to 33b facing the movement locus E of the substrate S in a direction orthogonal to the direction of the movement locus E.
  • the holes H are provided in one row. That is, the nozzle hole group HG in one row extends in a direction orthogonal to the direction of the movement locus E and with a length exceeding the width of the movement locus E.
  • the nozzle hole diameter D and the nozzle hole interval P are not particularly limited, but the diameter of the nozzle hole H is suitably about 10 to 100 ⁇ m, and the nozzle hole interval P is suitably about 100 to 200 ⁇ m.
  • the nozzle hole group HG may be provided in a plurality of rows of two or more.
  • a coating process under normal temperature and atmospheric pressure based on a predetermined program is performed. That is, as shown in FIGS. 6A and 6B and FIGS. 7A and 7B, the support base 40a is intermittently moved in the direction of the arrow M by the moving mechanism 40, and the first The micro droplets La, Lb, and Lc are intermittently ejected from the third inkjet heads 31b to 33b at regular time intervals to form the coating film L3 on the substrate S.
  • the first inkjet head 31b A small droplet La of the acidic monomer solution is discharged and applied onto the substrate S.
  • the coating film L1 acidic monomer solution as shown in FIG. 6 (B) is formed at one end S 1 side of the substrate S.
  • the nozzle hole H for discharging the micro droplet La is selected from the nozzle hole group HG in the first inkjet head 31b so that the micro droplet La is not discharged on the frame 11 and the stage 10.
  • the second and third inkjet heads 32b and 33b are the same applies to the second and third inkjet heads 32b and 33b.
  • the liquid surface of the coating film L3 becomes a gently concave curved surface. That is, the outer peripheral portion of the liquid surface of the coating film L3 is higher than the central portion.
  • the first to third ink jet heads 31b to 33b Droplet discharge stops sequentially. Thereafter, the support base 40a returns to the standby position, and the coating process ends.
  • the discharge amount of the acidic monomer solution (microdroplets La) and the basic monomer solution (microdroplets Lb) from the first and second inkjet heads 31b and 32b is the amount of the gel layer obtained after the gelling process.
  • the pH gradient in the longitudinal direction (arrow M direction) is adjusted to be a predetermined gradient.
  • coating process is performed when a control part controls each drive part based on a predetermined program.
  • the door of the case 50 is opened, the frame 11 containing the base material S is taken out and stored in the case for the gelation process, and the gelation process of the coating film L3 is performed at room temperature in the case. To do. Note that it takes about 3 to 5 hours to complete the gelation at room temperature.
  • an isoelectric focusing test device GP 1 in which a gel layer G 1 is formed on the substrate S is obtained (see FIG. 1A).
  • the method for drying the gel layer G 1 is not particularly limited, and examples thereof include a method of standing at room temperature, heating the gel layer G 1 with a heater, or drying the gel layer G 1 by blowing hot air. It is done.
  • the dried membrane D 1 may be carried out cooling step of cooling the -20 ° C. or less. Or you may perform a freeze-dry process instead of a drying process and a cooling process.
  • (Other embodiments) 1 the case where a coating film in a room temperature state is formed on the base material in the coating step is exemplified. However, the coating film is formed on the base material under cooling using an apparatus including a Peltier element and a tank cooling unit. It may be formed. In the first embodiment, the case where the coating film is formed in the air in the coating process is illustrated, but the coating film may be formed in a nitrogen atmosphere.
  • a gel material solution containing a polymerization initiator may be applied onto the base material.
  • a gel material solution containing a polymerization initiator in a cooled state is used so that gelation of the gel material solution containing a polymerization initiator does not proceed during the coating process.
  • the cooled monomer solution and the cooled polymerization initiator are mixed in the vicinity of the nozzle, and further, the vicinity of the nozzle is also cooled to cool the gel material solution containing the polymerization initiator before discharge.
  • the substrate may be cooled.
  • a water film is formed in advance on the substrate, and the monomer solution and the polymerization initiator may be individually applied onto the water film as in the first embodiment, or the gel material solution containing the polymerization initiator may be applied. . 4).
  • the first embodiment the case where the coating film L3 is formed by passing the substrate S once under the discharge head unit U is illustrated. However, the coating film L3 is formed by moving the substrate S one or more times. May be.
  • the application timing of the polymerization initiator can be set, for example, at every movement, at a predetermined movement, or at the last movement.

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Abstract

La présente invention concerne : un dispositif de test par électrophorèse par électrofocalisation qui permet d'effectuer une électrophorèse bidimensionnelle avec une fiabilité élevée ; et un procédé de production dudit dispositif de test. Le dispositif de test par électrophorèse par électrofocalisation présente un film sec obtenu en séchant une couche de gel formée sur un substrat, et est caractérisé en ce que, lorsqu'il est imbibé, le film sec devient une couche de gel satisfaisant aux conditions (1) à (4) ; à savoir : (1) la couche de gel présente des surfaces d'extrémité périphériques externes dans la direction verticale par rapport au substrat ; (2) les surfaces d'extrémité périphériques externes de la couche de gel sont sur le même plan que les surfaces d'extrémité périphériques externes du substrat ; (3) les sections périphériques externes de la couche de gel sont les plus épaisses, alors que la section centrale de la couche de gel est la plus fine ; et (4) l'épaisseur de la section centrale de la couche de gel représente de 80 à 90 % de l'épaisseur des sections périphériques externes.
PCT/JP2012/083782 2012-02-29 2012-12-27 Dispositif de test par électrophorèse par électrofocalisation et son procédé de production WO2013128772A1 (fr)

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JP2012044401A JP2013181785A (ja) 2012-02-29 2012-02-29 等電点電気泳動用試験具およびその製造方法
JP2012-044401 2012-02-29

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006258685A (ja) * 2005-03-18 2006-09-28 National Institute Of Advanced Industrial & Technology 二次元電気泳動法用試料注入器具及びそれを含む二次元電気泳動用装置並びに該装置を用いた二次元電気泳動法
JP2008164319A (ja) * 2006-12-27 2008-07-17 National Institute Of Advanced Industrial & Technology 電気泳動用乾燥媒体への試料の導入方法及びそのための器具
WO2011158520A1 (fr) * 2010-06-18 2011-12-22 シャープ株式会社 Procédé pour produire un instrument de réaction pour électrophorèse, appareil pour produire un instrument de réaction pour électrophorèse, base pour immobilisation de gel, instrument de réaction pour électrophorèse et kit pour électrophorèse

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006258685A (ja) * 2005-03-18 2006-09-28 National Institute Of Advanced Industrial & Technology 二次元電気泳動法用試料注入器具及びそれを含む二次元電気泳動用装置並びに該装置を用いた二次元電気泳動法
JP2008164319A (ja) * 2006-12-27 2008-07-17 National Institute Of Advanced Industrial & Technology 電気泳動用乾燥媒体への試料の導入方法及びそのための器具
WO2011158520A1 (fr) * 2010-06-18 2011-12-22 シャープ株式会社 Procédé pour produire un instrument de réaction pour électrophorèse, appareil pour produire un instrument de réaction pour électrophorèse, base pour immobilisation de gel, instrument de réaction pour électrophorèse et kit pour électrophorèse

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