WO2013065000A1 - Composant destiné à être utilisé dans un dispositif microfluidique tridimensionnel, dispositif microfluidique tridimensionnel et procédé de fabrication dudit composant - Google Patents

Composant destiné à être utilisé dans un dispositif microfluidique tridimensionnel, dispositif microfluidique tridimensionnel et procédé de fabrication dudit composant Download PDF

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Publication number
WO2013065000A1
WO2013065000A1 PCT/IB2012/056060 IB2012056060W WO2013065000A1 WO 2013065000 A1 WO2013065000 A1 WO 2013065000A1 IB 2012056060 W IB2012056060 W IB 2012056060W WO 2013065000 A1 WO2013065000 A1 WO 2013065000A1
Authority
WO
WIPO (PCT)
Prior art keywords
hydrophobic substance
porous material
component
microfluidic device
channel
Prior art date
Application number
PCT/IB2012/056060
Other languages
English (en)
Inventor
Ville Rautiainen
Kalle KEMPPAINEN
Matti JORMAKKA
Martti KAUPPI
Mikko OVASKA
Original Assignee
Phd Nordic Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phd Nordic Oy filed Critical Phd Nordic Oy
Publication of WO2013065000A1 publication Critical patent/WO2013065000A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/126—Paper
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Definitions

  • the invention is related generally to three-dimensional devices for handling microfluids.
  • Microfluidic devices form one category of these means.
  • microfluidic devices it is possible, when they are used in biotechnology or medicine, for example, to trigger a biochemical reaction by using a relatively small sample.
  • Three-dimensional microfluidic devices comprise at least two layers within which a fluid arriving in the device can travel in the plane of a layer. Characteristic of a three-dimensional microfluidic device is that the fluid can be transferred from one layer to another. The manufacture of a three-dimensional microfluidic system by using porous material requires that the porous layers are attached on top of each other. A three-dimensional microfluidic system manufactured by patterning with a hydrophobic substance on a porous material requires a separate, non-porous layer that is impervious to fluids and that is located between the fluid-transporting porous layers that are patterned with a hydrophobic substance.
  • this non-porous layer that is impervious to fluids is manufactured using a non-porous material, such as a two-sided tape or plastic, that is impervious to fluids (the term "fluid" in this context and in what follows refers to water or to any other liquid than water intended for use in any given application and which possibly contains water) .
  • a fluid in this context and in what follows refers to water or to any other liquid than water intended for use in any given application and which possibly contains water
  • the two-sided tape has to be perforated. During the perforating process, the adhesive on a two-sided tape tends mess up the
  • the known two-sided tapes are of relatively thick material. For this reason, the fluids' ability to flow from one layer to another in a three-dimensional microfluidic device such as that presented in international patent application publication WO 2010/102294 Al is not necessarily sufficiently good, as such, but it may be necessary at the holes to use separately prepared paper pieces for setting in the holes. The preparation of the paper pieces and their fixing to the holes further complicates the manufacture of a three-dimensional microfluidic device.
  • the purpose of the invention is to simplify the manufacture of a three-dimensional microfluidic device.
  • a component for use in a three-dimensional microfluidic device comprises :
  • a three-dimensional microfluidic device contains at least two layers made of a porous material, each of which contains at least one channel defined using a hydrophobic substance, implemented in such a way that a fluid permeating in a channel can travel along the channel from one layer to another by means of capillary action. At least one layer of a three-dimensional microfluidic device is implemented by means of a component for use in a three-dimensional microfluidic device of the invention.
  • a method of manufacturing a component for use in a three-dimensional microfluidic device comprises the following steps:
  • a three-dimensional microfluidic device can be implemented without a separate non-porous intermediate layer that is impervious to fluids, such as a two-sided tape.
  • a separate non-porous intermediate layer that is impervious to fluids is no longer separately added, and as a hydrophobic substance is now only spread in manufacturing the component, a clear advantage is achieved in manufacturing.
  • the hydrophobic substance tends to penetrate much deeper into the surface layer of the porous material, unlike a two-sided tape.
  • taping one difficult-to-control and messy work phase can thus be avoided, which significantly simplifies the
  • the most economical spreading method is printing or pressing, especially with solid ink technology or mask or screen printing technology.
  • the hydrophobic substance that is spread on the floor or ceiling area and the hydrophobic substance that is caused to permeate the porous material are together arranged to seal at least one floor or ceiling corner of at least one above- mentioned channel, the travel of a fluid from a channel in the component to an upper or lower layer of a three-dimensional
  • microfluidic device can be better controlled, because, in this way, an unwanted and unintended arrival of a fluid in a different layer at the location of a floor or ceiling corner can be better
  • the hydrophobic substance that is caused to permeate porous material is wax or contains wax, especially printing wax.
  • the hydrophobic substance that is spread to form the floor or ceiling area is wax or contains wax.
  • microfluidic device an embodiment that is based on wax or contains wax .
  • the hydrophobic substance permeating the porous material is caused to permeate the porous material by mask or screen printing technology or by heating, especially in the form of a pattern.
  • the hydrophobic substance spread to form a floor or ceiling area is spread on the surface of the porous material by printing or
  • pressing especially printing ith solid ink technology or mask or screen printing technology, and most advantageously in the form of a pattern .
  • At lea one protein or other test zone is arranged in connection with at least one channel. This is done most advantageously before the compilation of the three-dimensional microfluidic device, during compilation or after compilation.
  • hydrophobic substance to permeate a porous material, a change in the surface area of the porous material and/or the hydrophobic
  • the hydrophobic substance especially the relative expansion of the hydrophobic substance and/or the relative shrinkage of the porous material, is compensated so that the size of the hydrophobic substance spread to implement the floor and/or ceiling area of the porous material and the alignment in relation to the surface of the porous material will better correspond to the changed size of the hydrophobic substance that permeates the porous material.
  • the hydrophobic substance is caused to permeate the porous material by using heating technology, any insufficient sealing caused by a transformation of the hydrophobic substance or the component can be compensated.
  • the resulting three-dimensional microfluidic device will be less susceptible to leaks than one in which the
  • FIG 1 a first sheet printing pattern
  • FIG 2 a single model of the first sheet printing pattern
  • FIG 3 a cross-section III-III of one model of a component billet ;
  • FIG 4 a second sheet printing pattern
  • FIG 5 a single model of the second sheet printing pattern
  • FIG 6 a cross-section VI-VI of the first single component
  • FIG 7 a third sheet printing pattern
  • FIG 8 a single model of the third sheet printing pattern
  • FIG 9 a cross-section IX-IX of the second single component
  • FIG 10 the component billet made for the sheet after printing the first sheet printing pattern
  • FIG 11 the component billet of FIG 10 after the hydrophobic substance has permeated the component billet;
  • FIG 12 a component for use in a three-dimensional microfluidic device manufactured using the first component billet presented in FIG 11;
  • FIG 13 a component for use in a three-dimensional microfluidic device manufactured using the second component billet presented in FIG 11.
  • the same reference numbers refer to the same technical features in all drawings.
  • FIG 1 presents a first sheet printing pattern 10.
  • Sheet printing pattern 10 contains many single models 11, one of which is presented in more detail in FIG 2.
  • FIG 10 presents sheet la, on which sheet printing pattern 10 is printed. Wax is brought to sheet la by printing on it the desired patterns of hydrophobic substance 21, 22 with a Xerox Corp. Solid Ink technology printer - a Xerox Phaser 8560 or 8860 printer, for example, preferably with the "fine" print setting. As shown in FIG 10, the patterns printed on one face of the sheet can be seen faintly on the other face, at least if viewed against the light.
  • FIG 11 presents component billet 1 formed from sheet la presented in FIG 10.
  • Component billet 1 is manufactured from sheet la by causing the hydrophobic substance printed on it to permeate sheet la. In practice this can be implemented so that sheet la is put in an approximately 150°C oven for about two minutes. Under this
  • FIG 3 presents a cross-section of a single component billet.
  • a sheet of component billets is presented in FIG 11, which sheet thus contains many component billets.
  • the component billet of FIG 3 shows how hydrophobic barriers are formed in porous material 24 of component billet 1 at cross-section III-III of model 11 presented in FIG 2, which barriers are thus formed at those locations where hydrophobic substance 22 (i.e. the printing wax) is absorbed through the thickness of sheet 1.
  • a hydrophobic barrier is formed from the printed wax lines, the target width of which is at least 300 ⁇ . Lines that are thinner than this width do not contain enough wax to allow a hydrophilic barrier to be formed through the entire thickness of the sheet.
  • the hydrophobic barrier formed by means of hydrophobic substance 22 defines the boundaries of channel K.
  • the porous material 24 of the channel's interior 23 remains entirely or mostly free of hydrophobic substance.
  • the wax prints defining its boundary should be at a distance of at least 1100 ⁇ from each other.
  • the width of channel K will be about 560 ⁇ due to the heat treatment.
  • a hydrophilic channel can preferably be even thicker; most important is only that the fluid advances in channel K by capillary action.
  • the filter papers Whatman NO. 1, Ahlstrom grade 601 and Hahnemuehle Grade FP595 have proven to be very good as sheet materials.
  • the use of other paper grades is also possible, but the filter paper grades presented here have a pore size that is especially well suited for the absorption of fluids.
  • the basis weight (g/m2) of the mentioned filter papers and the form of the fiber matrix are advantageous for the intended purpose and allow the implementation of a device for handling microfluids, which device can be used without an expensive external pump.
  • the required wax line width is chosen in accordance with the sheet material to be used.
  • the above-presented wax line width (at least 300 ⁇ ) works with Whatman NO. 1 paper, but the other paper grades may require the use of a thicker line width.
  • FIG 4 presents the second sheet printing pattern 40.
  • the second sheet printing pattern 40 contains many single models 41, one of which is presented in more detail in FIG 5.
  • sheet printing pattern 40 is printed on component billet 1 presented in FIG 11.
  • hydrophobic substance 51 is spread on component billet 1.
  • sheet 2 of components for use in three-dimensional microfluidic devices is created. The printing is implemented most simply by using the above-described printing arrangement.
  • FIG 6 shows a cross-section of a single component formed on sheet 2.
  • a sheet of components is presented in FIG 12, from which sheet a single component is thus presented in FIG 6.
  • hydrophobic substance 51 has been added to the surface of the component on one side. This hydrophobic substance 51 comes from model 41 presented in FIG 5.
  • FIG 7 presents a third sheet printing pattern 70.
  • the third sheet printing pattern 70 also contains many single models 71, one of which is presented in more detail in FIG 8.
  • sheet printing pattern 70 is printed on sheet 2 presented in FIG 12.
  • hydrophobic substance 81 is spread on the surface of sheet 2.
  • FIG 9 shows a cross-section of a single component formed on sheet 3.
  • a sheet of components is now presented in FIG 13, one of which is thus presented in FIG 9.
  • hydrophobic substance 81 has been added to the surface of the component on the other side, too.
  • This hydrophobic substance 81 comes from model 71 presented in FIG 8.
  • Hydrophobic substance 51 of printing pattern 20 forms the ceilings M for the channel network.
  • Hydrophobic substance 81 of printing pattern 30 forms the floors L for the channel network.
  • channel K works better solely with floor layer L.
  • the side on which floor layer L is printed relative to the first printing has a significant influence. It has significance especially when three- dimensional microfluidic devices (which, by definition, comprise many layers) are made by this technique combined with gluing or folding and pressing.
  • component 2 for use in a three-dimensional microfluidic device is made so that solely floor layer L is printed, the print must be made on the same side of the printed sheet as the printing has been done in FIG 10. Then component 2 for use in a three-dimensional
  • microfluidic device will work better.
  • the invention is presented with the aid of the above exemplary embodiments.
  • the exemplary embodiments are not meant to limit the scope of the patent protection applied for, but the scope of protection can vary and differ from the exemplary embodiments within the framework of the attached claims and their legal equivalents .
  • insect waxes vegetable waxes, mineral waxes, petroleum waxes, microchrystalline waxes, synthetic waxes or combinations thereof may be used instead of, or in addition to, Xerox Corp.'s wax-based ink.
  • Candle wax may also be used.
  • Hydrophobic area 51, 81 may consist of or contain glue.
  • Each test zone P can include, in particular, one or more of the following: a protein assay, a cholesterol assay, a glucose assay and a bioassay.
  • a priming solution (0.20 L, 250-mM citrate buffer, pH 1.9, prepared in 92% water and 8% ethanol by volume) can be spotted in the protein test zone using a micro-pipette (VWR) and allowed to dry for 10 minutes at ambient temperature.
  • a reagent solution (0.20 L, 9-mM tetrabromophenol blue prepared in 95% ethanol and 5% water by volume) is spotted on top of the priming solution and dried for 10 minutes at ambient temperature. More detailed instructions for preparing protein and other assays can be found in patent application publication WO 2010/102294 Al and in particular in the documents referred to therein.

Abstract

Le composant (2, 3) ci-décrit, destiné à être utilisé dans un dispositif microfluidique tridimensionnel, est caractérisé en ce qu'il comprend : • au moins une couche (1) qui est formée par perméation d'une substance hydrophobe (21, 22) dans un matériau poreux (24) de façon que la substance hydrophobe (21, 22) soit absorbée dans le matériau poreux (24) de la couche (1) de manière à former au moins une limite (22) d'un canal (K) dépourvu de substance hydrophobe ; et • au moins une zone de plancher et/ou plafond de canal (51, 81) qui se trouve au moins partiellement sur, ou qui s'étend sur, le canal précité (K), et qui est formée par étalement d'une substance hydrophobe (51, 81) sur la surface du matériau (24) de la couche précitée (1) de façon que la zone de plancher (L) forme à sa position une barrière empêchant la migration d'un fluide sous la couche (1) et/ou que la zone de plafond (M) forme à sa position une barrière contre la migration d'un fluide au-dessus de la couche (1). La présente demande de brevet contient également une revendication indépendante concerne un dispositif microfluidique tridimensionnel et un procédé de fabrication d'un composant destiné à être utilisé dans ledit dispositif microfluidique tridimensionnel.
PCT/IB2012/056060 2011-11-01 2012-10-31 Composant destiné à être utilisé dans un dispositif microfluidique tridimensionnel, dispositif microfluidique tridimensionnel et procédé de fabrication dudit composant WO2013065000A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11187407.9A EP2589435A1 (fr) 2011-11-01 2011-11-01 Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel
EP11187407.9 2011-11-01

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WO2013065000A1 true WO2013065000A1 (fr) 2013-05-10

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WO (1) WO2013065000A1 (fr)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN114340776A (zh) * 2019-08-29 2022-04-12 佳能株式会社 微流路设备的制造方法

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US20160008812A1 (en) * 2013-02-28 2016-01-14 Rie Kobayashi Fluidic device and fabrication method thereof, and thermal transfer medium for fluidic device fabrication
US11642669B2 (en) 2017-10-18 2023-05-09 Group K Diagnostics, Inc. Single-layer microfluidic device and methods of manufacture and use thereof
JP2021175970A (ja) * 2020-04-28 2021-11-04 デクセリアルズ株式会社 検査チップ、及びその製造方法
CN112973813B (zh) * 2021-02-10 2023-03-14 齐鲁工业大学 一种用于分离富集外泌体的微流控芯片及其制作方法

Citations (7)

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Publication number Priority date Publication date Assignee Title
US20090298191A1 (en) * 2006-10-18 2009-12-03 President And Fellows Of Harvard College Lateral Flow and Flow-through Bioassay Devices Based On Patterned Porous Media, Methods of Making Same, and Methods of Using Same
WO2010003188A1 (fr) * 2008-07-11 2010-01-14 Monash University Procédé de fabrication de systèmes microfluidiques
WO2010022324A2 (fr) * 2008-08-22 2010-02-25 President And Fellows Of Harvard College Procédés de création de motifs sur du papier
WO2010102294A1 (fr) 2009-03-06 2010-09-10 President And Fellows Of Harvard College Méthodes de micro-impression de microfluides à base de papier
US20110105360A1 (en) * 2008-03-27 2011-05-05 President And Fellows Of Harvard College Paper-based cellular arrays
WO2011073519A1 (fr) * 2009-12-15 2011-06-23 Teknologian Tutkimuskeskus Vtt Procédé de fabrication de structures de guidage de flux de liquide vers des substrats poreux
WO2011097677A1 (fr) * 2010-02-12 2011-08-18 Monash University Plaques à multiples microzones imprimées

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090298191A1 (en) * 2006-10-18 2009-12-03 President And Fellows Of Harvard College Lateral Flow and Flow-through Bioassay Devices Based On Patterned Porous Media, Methods of Making Same, and Methods of Using Same
US20110105360A1 (en) * 2008-03-27 2011-05-05 President And Fellows Of Harvard College Paper-based cellular arrays
WO2010003188A1 (fr) * 2008-07-11 2010-01-14 Monash University Procédé de fabrication de systèmes microfluidiques
WO2010022324A2 (fr) * 2008-08-22 2010-02-25 President And Fellows Of Harvard College Procédés de création de motifs sur du papier
WO2010102294A1 (fr) 2009-03-06 2010-09-10 President And Fellows Of Harvard College Méthodes de micro-impression de microfluides à base de papier
WO2011073519A1 (fr) * 2009-12-15 2011-06-23 Teknologian Tutkimuskeskus Vtt Procédé de fabrication de structures de guidage de flux de liquide vers des substrats poreux
WO2011097677A1 (fr) * 2010-02-12 2011-08-18 Monash University Plaques à multiples microzones imprimées

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114340776A (zh) * 2019-08-29 2022-04-12 佳能株式会社 微流路设备的制造方法
CN114340776B (zh) * 2019-08-29 2024-02-13 佳能株式会社 微流路设备的制造方法
US11970390B2 (en) 2019-08-29 2024-04-30 Canon Kabushiki Kaisha Method for producing microchannel device

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