WO2011097677A1 - Plaques à multiples microzones imprimées - Google Patents

Plaques à multiples microzones imprimées Download PDF

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Publication number
WO2011097677A1
WO2011097677A1 PCT/AU2011/000138 AU2011000138W WO2011097677A1 WO 2011097677 A1 WO2011097677 A1 WO 2011097677A1 AU 2011000138 W AU2011000138 W AU 2011000138W WO 2011097677 A1 WO2011097677 A1 WO 2011097677A1
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WO
WIPO (PCT)
Prior art keywords
microzone
pattern
substrate
zone
ink
Prior art date
Application number
PCT/AU2011/000138
Other languages
English (en)
Inventor
Wei Shen
Xu Li
Junfei Tian
Original Assignee
Monash University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2010900564A external-priority patent/AU2010900564A0/en
Application filed by Monash University filed Critical Monash University
Publication of WO2011097677A1 publication Critical patent/WO2011097677A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezo-electric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility

Definitions

  • the present invention is generally directed to multi-assay analyses which are widely used in chemical and biological analysis, drug evaluation, ELISA and large scale disease screening, and in particular to a multi-zone microzone plate, and method of producing thereof.
  • multi-assay work is essential, as it provides (a) rapid evaluation of certain chemical and biochemical reactions under many different conditions; (b) rapid results to determine anti-body and antigen in human samples; and (c) rapid evaluation.
  • multi-assay systems can perform a large number of experiments in a short time, it has become a reliable tool in medical tests, biomedical research and drug research.
  • Multi-assay experiments are primarily done by using 96-well plates, which is the most widely used consumable item in those tests.
  • the 96-well plates are typically made by polymer moulding, are expensive and require a significant amount of samples (10-500 micro litres).
  • the current market price of the 96-well plates are around AU$10 and are therefore not cheap enough to be used for underdeveloped countries/regions for human healthcare and disease screening.
  • Carrilho ei a/ [Emanuel Carrilho, Scott T. Phillips, Sarah J. Vella, Andrew W. Martinez and George M. Whitesides, Anal. Chem., 2009, 81 (15), pp 5990- 5998] have proposed a fabrication method for paper-based 96- and 384- zone plate. Their method is to apply photolithographic photo resist to a paper sheet to form hydrophobic barriers which encircle hydrophilic paper zones. Those paper zones are capable of absorbing liquid samples. Paper, which is made of cellulose fibres, is an excellent material for colorimetric analysis. However, paper can deform when experiencing repeated wetting and rewetting cycles.
  • a multi- zone microzone plate for use in multi-assay analyses including a substrate having a hydrophobic surface, and a microzone pattern printed on the substrate surface, wherein the microzone pattern includes hyd rophilic material therein.
  • a method of producing a multi-zone microzone plate for use in multi-assay analyses including printing a microzone pattern on a hydrophobic surface of a substrate, the microzone pattern including hydrophilic material therein.
  • the present invention provides a novel method to make low-cost, disposable multi-zone microzone plates by means of printing.
  • the multi-zone microzone plate made using this method is capable of conducting multi-sample assay for chemical and biological analysis and ELISA-type analysis.
  • the present invention preferably uses polymer films of thickness of more than 10 microns as a substrate.
  • water repellent paper such as photographic paper, wax paper, strongly sized paper, or non-woven non- cellulosic paper as used for example for strong envelopes, may be used as the substrate.
  • the films or paper can be transparent, opaque or having any background colour for purpose of sample colour or fluorescent detections.
  • the present invention can also use laminated polymer films of thickness of more than 10 microns as a substrate.
  • the laminated polymer films may have any background colour for purpose of sample colour or fluorescent detections.
  • the water-resistant paper may have a thickness of more than 10 microns, translucent, opaque or have a background colour.
  • One preferred method is to use UV-curable or any other types of energy curable fluids or varnish as the printing ink; these fluids and varnish include I R curable, electron beam curable, microwave curing, and moisture curable fluids.
  • This method may use contact (or non-contact printing i.e. digital inkjet printing) to print the above mentioned ink onto the substrate.
  • Flexographic, gravure, lithographic, xerographic, wax printing, ink jet printing processes, etc. can therefore be used to print a microzone pattern of ink onto the substrate.
  • This method preferably uses cellulose powder (or any polymer powder or mineral powder, or any synthetic or natural inorganic or organic powder) to form a porous pattern over the printed UV-curable ink.
  • the powder sticks to the printed but uncured ink film and therefore forms porous microzone pattern.
  • the powder can be single component, or a mixture of multi-component powder, or surface modified powder.
  • One or more different hydrophilic powders may be laid in layers to the ink or varnish.
  • This method may use a UV ink dryer, or any other type of ink curing device that matches the drying mechanism of the ink, including an IR dryer and electron beam dryer, microwave dryer, to cure the ink and therefore fix the porous cellulose microzone pattern on the film.
  • a UV ink dryer or any other type of ink curing device that matches the drying mechanism of the ink, including an IR dryer and electron beam dryer, microwave dryer, to cure the ink and therefore fix the porous cellulose microzone pattern on the film.
  • Another preferred method is to use a suitable aqueous or non-aqueous, organic or non-organic binder system and cellulose (or other, polymer or mineral) powder to form printable paste "inks".
  • a suitable aqueous or non-aqueous, organic or non-organic binder system and cellulose (or other, polymer or mineral) powder to form printable paste "inks”.
  • Contact printing and patterned coating methods are used to print or coat the paste ink onto polymer film to form the required microzone pattern.
  • the evaporation of the solvent or water (either naturally or aided by thermal and other forms of energy) from the paste ink will allow the ink to cure on the film and form a porous microzone pattern.
  • the binder in the ink promotes adhesion of between the dried ink and the film.
  • the microzone pattern created by printing may be porous and able to absorb small quantity of liquid.
  • the microzone pattern created by printing according to the present invention can be wetted and rewetted without deforming. Chemical, biological reactions can be performed in the microzones of the plate and the colour developed in these microzones can be measured using a camera or a scanner and any suitable software such as PhotoShop (Registered Trademark of Adobe Systems).
  • chemical, biological reactions can be performed in the microzones and the colour developed in the microzones can be measured using suitable reflective or transmission optical densitometers.
  • chemical, biological reactions can be performed in the microzones and fluorescent signal developed in the microzones can be measured using a plate reader or any other suitable type of fluorescence measurement apparatus.
  • chemical, biological reactions can be performed in the microzones and detection reaction can be monitored by means or electrochemical detection.
  • Figure 1 is a photo showing a 96-zone microzone plate fabricated on polypropylene overhead transparency film according to the present invention
  • Figure 2 is photo showing a porous microzone pattern printed using a paste made of cellulose powder and starch solution according to the present invention
  • Figure 3 is a photo of a printed 96-zone microzone plate according to the present invention showing the colorimetric differences of liquids added to the plate.
  • Figure 4 is a graph sowing the reflective colour density measurement of colour density of the inked zones of the microzone plate of Figure 3;
  • Figure 5 is a plan of using a printed microzone plate according to the present invention to determine the chemical interference of UA to N0 2 ;
  • Figure 6 is a photo of the interference analysis of a microzone plate according to the present invention.
  • Figure 7 is a graph showing four calibration curves of N0 2 " in the presence of different concentrations of UA.
  • Example 1 Printing of microzone plate using polymer film, UV curable ink based and cellulose powder
  • a 96-zone printing master was made using rubber. The size of the dots on the master is 3 mm in diameter.
  • a UV curable post print varnish (UV 412, Flint Inks) was used as the ink.
  • the rubber printing master was inked with the varnish and printed onto an overhead transparency by contact.
  • the printed microzone pattern on the transparency film was then exposed to cellulose powder (Microgranullar cellulose, Aldrich). Cellulose powder stuck to the printed varnish microzone pattern, forming a pattern of cellulose powder.
  • the microzone plate was then exposed to UV radiation (UV ink dryer), the printed varnish was cured. Curing of the varnish allows cellulose powder to strongly stick onto the microzone plate, forming a porous surface of the microzone pattern.
  • Figure 1 shows the printed microzone plate on an overhead transparency.
  • Example 2 Printing of cellulose-binder paste ink on the polymer film
  • a cellulose powder pattern were printed using a cellulose-binder paste "ink” onto an overhead transparency film using contact printing.
  • a starch solution (0.5%, w/w) was cooked at 95°C until the solution became transparent; the solution was left to cool down to the room temperature.
  • One gram of cellulose powder was mixed with 30 ml of starch solution aided by strong stirring to form a printable paste cellulose "ink”.
  • a rubber master was used for contact printing and the dot size on the master was 2 mm diameter.
  • the printed pattern was let dry.
  • a blue aqueous liquid was then introduced from the centre of the pattern to demonstrate the wettability of the pattern.
  • the pattern was rapidly and fully wetted by the blue liquid.
  • Figure 2 shows the printed pattern fully wetted by the blue liquid. (The printed pattern can also handle organic solvent, and can use oil to match refractive index for transmission analysis)
  • Example 3 Liquid sample receptive ability and colorimetric development ability of the printed 96-zone pattern
  • FIG. 1 shows the photo colorimetric development of all liquids in the 96-zone pattern showing the colorimetric differences.
  • Figure 4 shows reflective density measurements of the colour density of the inked zones.
  • Example 4 - Reaction interference study using the printed 96-zone plate Uric acid (IA) and N0 2 " are two important biomarkers of several human health conditions. They present in human body fluid such as blood and saliva. Although detection methods for UA and N0 2 " are available in the literature, their detection interference must be investigated. This is because that these two biological species co-exist in human samples.
  • FIG. 5 shows the division of the printed 96-zone plate into four regions, region 1 , 2, 3 and 4. Each region tests a series of N0 2 ⁇ solution in the presence of UA of a fixed concentration. Therefore the four regions test a series of N0 2 " samples with the UA concentration ranging from 0- 1000 ⁇ .
  • Figure 6 shows the photo of the printed 96-zone plate filled with analyte (N0 2 ) and interferent (UA).
  • Figure 7 shows the colour density measurement of the tests. Colour density measurements of N0 2 " were plotted against N0 2 " concentrations, with the interference (UA) concentration in the legend box to provide four calibration curves.
  • Results show that UA does not have significant interference to N0 2 " measurement, and the present of UA in N0 2 " samples does not cause significant error of measurement.
  • This result is in agreement with the tests published by the Harvard Medical School [Blicharz TM, Rissin DM, Bowden M, Hayman RB, DiCesare C, Bhatia JS, Grand-Pierre N, Siqueira WL, Helmerhorst EJ, Loscaizo J, Oppenheim FG, Walt DR (2008) Clin Chem 54:1473-1480].
  • This example shows the capability of the printed plates to sort out chemical interference issues under many different conditions very quickly. The printed plate is therefore able to conduct multi-chemical reactions in a short time.

Abstract

La présente invention concerne une plaque à multiples microzones, destinée à servir dans des analyses à dosages multiples, comprenant un substrat ayant une surface hydrophobe et un motif à microzones imprimé sur la surface du substrat, le motif à microzones contenant un matériau hydrophile. L'invention propose également un procédé de production associé.
PCT/AU2011/000138 2010-02-12 2011-02-11 Plaques à multiples microzones imprimées WO2011097677A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2010900564 2010-02-12
AU2010900564A AU2010900564A0 (en) 2010-02-12 Printed Multi-Zone Microzone Plates

Publications (1)

Publication Number Publication Date
WO2011097677A1 true WO2011097677A1 (fr) 2011-08-18

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2589435A1 (fr) * 2011-11-01 2013-05-08 PHD Nordic Oy Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel
CN103879953A (zh) * 2014-04-02 2014-06-25 东南大学 一种基于蜡的刮涂图案化方法
WO2015093050A1 (fr) * 2013-12-18 2015-06-25 Canon Kabushiki Kaisha Procédé permettant de fabriquer un motif, appareil de fabrication permettant de fabriquer un motif, procédé permettant de fabriquer un corps structural et son appareil de fabrication
GB2543619A (en) * 2015-10-16 2017-04-26 Univ Oxford Innovation Ltd Microfluidic arrangements
JP2017181333A (ja) * 2016-03-31 2017-10-05 シチズンファインデバイス株式会社 試料積載プレート及びその製造方法
US9823241B2 (en) 2012-07-18 2017-11-21 Koninklijke Philips N.V. Processing of a sample fluid with target components
WO2018033736A1 (fr) * 2016-08-18 2018-02-22 Oxford University Innovation Limited Procédés et appareil pour commander un écoulement dans un agencement microfluidique
CN111595843A (zh) * 2020-05-20 2020-08-28 中国科学院新疆理化技术研究所 一种用于阵列化比色分析的粘性采样检测纸的制备方法和用途
US11207455B2 (en) 2018-05-14 2021-12-28 Oregon State University Membrane device for blood separation and methods of making and using the same
US11311874B2 (en) 2017-06-07 2022-04-26 Oregon Slate University Polymer-based well plate devices and fluidic systems and methods of making and using the same
US11389796B2 (en) 2016-05-31 2022-07-19 Oregon State University Fluidic devices for chromatographic separation and methods of making and using the same
US11590503B2 (en) 2015-10-16 2023-02-28 Oxford University Innovation Limited Microfluidic arrangements

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EP1284495A2 (fr) * 2001-08-17 2003-02-19 Micromass Limited Spectromètre de masse
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WO2009039286A2 (fr) * 2007-09-18 2009-03-26 Primorigen Biosciences, Llc Puces multiplexées sans cadre
WO2009120963A2 (fr) * 2008-03-27 2009-10-01 President And Fellows Of Harvard College Réseaux cellulaires à base de papier

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WO1998041323A1 (fr) * 1997-03-14 1998-09-24 Moxtek, Inc. Porte-echantillon perfectionne a film mince
WO1998045406A1 (fr) * 1997-04-09 1998-10-15 Minnesota Mining And Manufacturing Company Procede et dispositifs pour diviser des liquides d'echantillons biologiques en microvolumes
US6287872B1 (en) * 1997-12-11 2001-09-11 Bruker Daltonik Gmbh Sample support plates for Maldi mass spectrometry including methods for manufacture of plates and application of sample
US20020045270A1 (en) * 2000-09-01 2002-04-18 Martin Schurenberg Structured biosample support plates for mass spectroscopic analyses and procedures for manufacturing and use
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WO2003031054A2 (fr) * 2001-10-05 2003-04-17 Surmodics, Inc. Reseaux presentant des configurations en grappes et procedes de fabrication et d'utilisation de tels reseaux
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Cited By (23)

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Publication number Priority date Publication date Assignee Title
WO2013065000A1 (fr) * 2011-11-01 2013-05-10 Phd Nordic Oy Composant destiné à être utilisé dans un dispositif microfluidique tridimensionnel, dispositif microfluidique tridimensionnel et procédé de fabrication dudit composant
EP2589435A1 (fr) * 2011-11-01 2013-05-08 PHD Nordic Oy Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel
US9823241B2 (en) 2012-07-18 2017-11-21 Koninklijke Philips N.V. Processing of a sample fluid with target components
WO2015093050A1 (fr) * 2013-12-18 2015-06-25 Canon Kabushiki Kaisha Procédé permettant de fabriquer un motif, appareil de fabrication permettant de fabriquer un motif, procédé permettant de fabriquer un corps structural et son appareil de fabrication
CN105829117A (zh) * 2013-12-18 2016-08-03 佳能株式会社 图案形成方法、图案形成用制造设备、结构体形成方法和用于其的制造设备
RU2607744C2 (ru) * 2013-12-18 2017-01-10 Кэнон Кабусики Кайся Способ изготовления рисунка, аппарат для изготовления рисунка, способ изготовления структурного тела и аппарат для его изготовления
US9789704B2 (en) 2013-12-18 2017-10-17 Canon Kabushiki Kaisha Method for manufacturing a pattern, manufacturing apparatus for manufacturing a pattern, method for manufacturing structural body and manufacturing apparatus therefor
US9919537B2 (en) 2013-12-18 2018-03-20 Canon Kabushiki Kaisha Method for manufacturing a pattern, manufacturing apparatus for manufacturing a pattern, method for manufacturing structural body and manufacturing apparatus therefor
CN103879953A (zh) * 2014-04-02 2014-06-25 东南大学 一种基于蜡的刮涂图案化方法
GB2543619A (en) * 2015-10-16 2017-04-26 Univ Oxford Innovation Ltd Microfluidic arrangements
GB2543619B (en) * 2015-10-16 2020-04-08 Univ Oxford Innovation Ltd A method of rehydrating a microfluidic arrangement
US11590503B2 (en) 2015-10-16 2023-02-28 Oxford University Innovation Limited Microfluidic arrangements
JP2017181333A (ja) * 2016-03-31 2017-10-05 シチズンファインデバイス株式会社 試料積載プレート及びその製造方法
US11389796B2 (en) 2016-05-31 2022-07-19 Oregon State University Fluidic devices for chromatographic separation and methods of making and using the same
WO2018033736A1 (fr) * 2016-08-18 2018-02-22 Oxford University Innovation Limited Procédés et appareil pour commander un écoulement dans un agencement microfluidique
US10967371B2 (en) 2016-08-18 2021-04-06 Oxford University Innovation Limited Methods and apparatus for controlling flow in a microfluidic arrangement, and a microfluidic arrangement
CN109661271B (zh) * 2016-08-18 2021-08-31 牛津大学科技创新有限公司 采用微流体布置的流动驱动方法和设备
US11148136B2 (en) 2016-08-18 2021-10-19 Oxford University Innovation Limited Methods and apparatus for driving flow in a microfluidic arrangement
CN109661271A (zh) * 2016-08-18 2019-04-19 牛津大学科技创新有限公司 采用微流体布置的流动驱动方法和设备
WO2018033734A1 (fr) * 2016-08-18 2018-02-22 Oxford University Innovation Limited Procédés et appareil de contrôle d'écoulement dans un agencement microfluidique, et un agencement microfluidique
US11311874B2 (en) 2017-06-07 2022-04-26 Oregon Slate University Polymer-based well plate devices and fluidic systems and methods of making and using the same
US11207455B2 (en) 2018-05-14 2021-12-28 Oregon State University Membrane device for blood separation and methods of making and using the same
CN111595843A (zh) * 2020-05-20 2020-08-28 中国科学院新疆理化技术研究所 一种用于阵列化比色分析的粘性采样检测纸的制备方法和用途

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