EP2589435A1 - Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel - Google Patents
Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel Download PDFInfo
- Publication number
- EP2589435A1 EP2589435A1 EP11187407.9A EP11187407A EP2589435A1 EP 2589435 A1 EP2589435 A1 EP 2589435A1 EP 11187407 A EP11187407 A EP 11187407A EP 2589435 A1 EP2589435 A1 EP 2589435A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- hydrophobic substance
- porous material
- microfluidic device
- component
- channel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0825—Test strips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0874—Three dimensional network
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
Definitions
- the invention is related generally to three-dimensional devices for handling microfluids.
- Microfluidic devices form one category of these means.
- microfluidic devices it is possible, when they are used in biotechnology or medicine, for example, to trigger a biochemical reaction by using a relatively small sample.
- Three-dimensional microfluidic devices comprise at least two layers within which a fluid arriving in the device can travel in the plane of a layer. Characteristic of a three-dimensional microfluidic device is that the fluid can be transferred from one layer to another. The manufacture of a three-dimensional microfluidic system by using porous material requires that the porous layers are attached on top of each other.
- a three-dimensional microfluidic system manufactured by patterning with a hydrophobic substance on a porous material requires a separate, non-porous layer that is impervious to fluids and that is located between the fluid-transporting porous layers that are patterned with a hydrophobic substance.
- this non-porous layer that is impervious to fluids is manufactured using a non-porous material, such as a two-sided tape or plastic, that is impervious to fluids (the term "fluid" in this context and in what follows refers to water or to any other liquid than water intended for use in any given application and which possibly contains water).
- a fluid in this context and in what follows refers to water or to any other liquid than water intended for use in any given application and which possibly contains water.
- the known two-sided tapes are of relatively thick material. For this reason, the fluids' ability to flow from one layer to another in a three-dimensional microfluidic device such as that presented in international patent application publication WO 2010/102294 A1 is not necessarily sufficiently good, as such, but it may be necessary at the holes to use separately prepared paper pieces for setting in the holes. The preparation of the paper pieces and their fixing to the holes further complicates the manufacture of a three-dimensional microfluidic device.
- the purpose of the invention is to simplify the manufacture of a three-dimensional microfluidic device.
- a component for use in a three-dimensional microfluidic device comprises:
- a three-dimensional microfluidic device contains at least two layers made of a porous material, each of which contains at least one channel defined using a hydrophobic substance, implemented in such a way that a fluid permeating in a channel can travel along the channel from one layer to another by means of capillary action. At least one layer of a three-dimensional microfluidic device is implemented by means of a component for use in a three-dimensional microfluidic device of the invention.
- a method of manufacturing a component for use in a three-dimensional microfluidic device comprises the following steps:
- a three-dimensional microfluidic device can be implemented without a separate non-porous intermediate layer that is impervious to fluids, such as a two-sided tape.
- a separate non-porous intermediate layer that is impervious to fluids is no longer separately added, and as a hydrophobic substance is now only spread in manufacturing the component, a clear advantage is achieved in manufacturing.
- the hydrophobic substance tends to penetrate much deeper into the surface layer of the porous material, unlike a two-sided tape.
- the most economical spreading method is printing or pressing, especially with solid ink technology or mask or screen printing technology.
- the hydrophobic substance that is spread on the floor or ceiling area and the hydrophobic substance that is caused to permeate the porous material are together arranged to seal at least one floor or ceiling corner of at least one above-mentioned channel, the travel of a fluid from a channel in the component to an upper or lower layer of a three-dimensional microfluidic device can be better controlled, because, in this way, an unwanted and unintended arrival of a fluid in a different layer at the location of a floor or ceiling corner can be better prevented.
- the hydrophobic substance that is caused to permeate a porous material is wax or contains wax, especially printing wax.
- the hydrophobic substance that is spread to form the floor or ceiling area is wax or contains wax.
- wax as a substance delimiting the channel of a three-dimensional microfluidic device has been studied so much that practitioners can easily accept, for a three-dimensional microfluidic device, an embodiment that is based on wax or contains wax.
- the hydrophobic substance permeating the porous material is caused to permeate the porous material by mask or screen printing technology or by heating, especially in the form of a pattern.
- the hydrophobic substance spread to form a floor or ceiling area is spread on the surface of the porous material by printing or pressing, especially printing with solid ink technology or mask or screen printing technology, and most advantageously in the form of a pattern.
- At least one protein or other test zone is arranged in connection with at least one channel. This is done most advantageously before the compilation of the three-dimensional microfluidic device, during compilation or after compilation.
- a change in the surface area of the porous material and/or the hydrophobic substance, especially the relative expansion of the hydrophobic substance and/or the relative shrinkage of the porous material is compensated so that the size of the hydrophobic substance spread to implement the floor and/or ceiling area of the porous material and the alignment in relation to the surface of the porous material will better correspond to the changed size of the hydrophobic substance that permeates the porous material.
- any insufficient sealing caused by a transformation of the hydrophobic substance or the component can be compensated.
- the resulting three-dimensional microfluidic device will be less susceptible to leaks than one in which the transformation of components is not taken into account during manufacture.
- FIG 1 presents a first sheet printing pattern 10.
- Sheet printing pattern 10 contains many single models 11, one of which is presented in more detail in FIG 2 .
- FIG 10 presents sheet 1a, on which sheet printing pattern 10 is printed. Wax is brought to sheet 1a by printing on it the desired patterns of hydrophobic substance 21, 22 with a Xerox Corp. Solid Ink technology printer - a Xerox Phaser 8560 or 8860 printer, for example, preferably with the "fine" print setting. As shown in FIG 10 , the patterns printed on one face of the sheet can be seen faintly on the other face, at least if viewed against the light.
- FIG 11 presents component billet 1 formed from sheet 1a presented in FIG 10 .
- Component billet 1 is manufactured from sheet 1a by causing the hydrophobic substance printed on it to permeate sheet 1a. In practice this can be implemented so that sheet 1a is put in an approximately 150°C oven for about two minutes. Under this influence, the wax used in the printing will melt. In that case, the wax will be absorbed into the sheet through the thickness of sheet 1a.
- FIG 3 presents a cross-section of a single component billet.
- a sheet of component billets is presented in FIG 11 , which sheet thus contains many component billets.
- the component billet of FIG 3 shows how hydrophobic barriers are formed in porous material 24 of component billet 1 at cross-section III-III of model 11 presented in FIG 2 , which barriers are thus formed at those locations where hydrophobic substance 22 (i.e. the printing wax) is absorbed through the thickness of sheet 1.
- a hydrophobic barrier is formed from the printed wax lines, the target width of which is at least 300 pm. Lines that are thinner than this width do not contain enough wax to allow a hydrophilic barrier to be formed through the entire thickness of the sheet.
- the hydrophobic barrier formed by means of hydrophobic substance 22 defines the boundaries of channel K.
- the porous material 24 of the channel's interior 23 remains entirely or mostly free of hydrophobic substance.
- the wax prints defining its boundary should be at a distance of at least 1100 ⁇ m from each other. In this case the width of channel K will be about 560 ⁇ m due to the heat treatment.
- a hydrophilic channel can preferably be even thicker; most important is only that the fluid advances in channel K by capillary action.
- the filter papers Whatman NO. 1, Ahlstrom grade 601 and Hahnemuehle Grade FP595 have proven to be very good as sheet materials.
- the use of other paper grades is also possible, but the filter paper grades presented here have a pore size that is especially well suited for the absorption of fluids.
- the basis weight (g/m2) of the mentioned filter papers and the form of the fiber matrix are advantageous for the intended purpose and allow the implementation of a device for handling microfluids, which device can be used without an expensive external pump.
- the required wax line width is chosen in accordance with the sheet material to be used.
- the above-presented wax line width (at least 300 ⁇ m) works with Whatman NO. 1 paper, but the other paper grades may require the use of a thicker line width.
- FIG 4 presents the second sheet printing pattern 40.
- the second sheet printing pattern 40 contains many single models 41, one of which is presented in more detail in FIG 5 .
- sheet printing pattern 40 is printed on component billet 1 presented in FIG 11 .
- hydrophobic substance 51 is spread on component billet 1.
- sheet 2 of components for use in three-dimensional microfluidic devices is created. The printing is implemented most simply by using the above-described printing arrangement.
- FIG 6 shows a cross-section of a single component formed on sheet 2.
- a sheet of components is presented in FIG 12 , from which sheet a single component is thus presented in FIG 6 .
- hydrophobic substance 51 has been added to the surface of the component on one side. This hydrophobic substance 51 comes from model 41 presented in FIG 5 .
- FIG 7 presents a third sheet printing pattern 70.
- the third sheet printing pattern 70 also contains many single models 71, one of which is presented in more detail in FIG 8 .
- sheet printing pattern 70 is printed on sheet 2 presented in FIG 12 .
- hydrophobic substance 81 is spread on the surface of sheet 2.
- sheet 3 of components for use in three-dimensional microfluidic devices is created. The printing is implemented most simply by using the above-described printing arrangement.
- FIG 9 shows a cross-section of a single component formed on sheet 3.
- a sheet of components is now presented in FIG 13 , one of which is thus presented in FIG 9 .
- hydrophobic substance 81 has been added to the surface of the component on the other side, too. This hydrophobic substance 81 comes from model 71 presented in FIG 8 .
- Hydrophobic substance 51 of printing pattern 20 forms the ceilings M for the channel network.
- Hydrophobic substance 81 of printing pattern 30 forms the floors L for the channel network.
- channel K works better solely with floor layer L.
- the side on which floor layer L is printed relative to the first printing has a significant influence. It has significance especially when three-dimensional microfluidic devices (which, by definition, comprise many layers) are made by this technique combined with gluing or folding and pressing.
- component 2 for use in a three-dimensional microfluidic device is made so that solely floor layer L is printed, the print must be made on the same side of the printed sheet as the printing has been done in FIG 10 . Then component 2 for use in a three-dimensional microfluidic device will work better.
- insect waxes vegetable waxes, mineral waxes, petroleum waxes, microchrystalline waxes, synthetic waxes or combinations thereof may be used instead of, or in addition to, Xerox Corp.'s wax-based ink.
- Candle wax may also be used.
- Hydrophobic area 51, 81 may consist of or contain glue.
- Each test zone P can include, in particular, one or more of the following: a protein assay, a cholesterol assay, a glucose assay and a bioassay.
- a priming solution (0.20 pL, 250-mM citrate buffer, pH 1.9, prepared in 92% water and 8% ethanol by volume) can be spotted in the protein test zone using a micro-pipette (VWR) and allowed to dry for 10 minutes at ambient temperature.
- a reagent solution (0.20 ⁇ L, 9-mM tetrabromophenol blue prepared in 95% ethanol and 5% water by volume) is spotted on top of the priming solution and dried for 10 minutes at ambient temperature.
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11187407.9A EP2589435A1 (fr) | 2011-11-01 | 2011-11-01 | Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel |
PCT/IB2012/056060 WO2013065000A1 (fr) | 2011-11-01 | 2012-10-31 | Composant destiné à être utilisé dans un dispositif microfluidique tridimensionnel, dispositif microfluidique tridimensionnel et procédé de fabrication dudit composant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11187407.9A EP2589435A1 (fr) | 2011-11-01 | 2011-11-01 | Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel |
Publications (1)
Publication Number | Publication Date |
---|---|
EP2589435A1 true EP2589435A1 (fr) | 2013-05-08 |
Family
ID=47326260
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP11187407.9A Withdrawn EP2589435A1 (fr) | 2011-11-01 | 2011-11-01 | Composant à utiliser dans un dispositif microfluide tridimensionnel, dispositif microfluide tridimensionnel et procédé de fabrication d'un dispositif microfluide tridimensionnel |
Country Status (2)
Country | Link |
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EP (1) | EP2589435A1 (fr) |
WO (1) | WO2013065000A1 (fr) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2962116A4 (fr) * | 2013-02-28 | 2016-08-10 | Ricoh Co Ltd | Dispositif liquide et procédé de fabrication de celui-ci et véhicule de transfert de chaleur pour la fabrication du dispositif liquide |
CN112973813A (zh) * | 2021-02-10 | 2021-06-18 | 齐鲁工业大学 | 一种用于分离富集外泌体的微流控芯片及其制作方法 |
EP3697537A4 (fr) * | 2017-10-18 | 2021-10-20 | Group K Diagnostics, Inc. | Dispositif microfluidique monocouche et ses procédés de fabrication et d'utilisation |
EP4145137A4 (fr) * | 2020-04-28 | 2024-04-24 | Dexerials Corporation | Puce de test et son procédé de fabrication |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7541864B2 (ja) | 2019-08-29 | 2024-08-29 | キヤノン株式会社 | マイクロ流路デバイスの製造方法 |
BR112022002527A2 (pt) | 2019-08-29 | 2022-05-10 | Canon Kk | Método para produção de dispositivo de microcanal |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090298191A1 (en) * | 2006-10-18 | 2009-12-03 | President And Fellows Of Harvard College | Lateral Flow and Flow-through Bioassay Devices Based On Patterned Porous Media, Methods of Making Same, and Methods of Using Same |
WO2010003188A1 (fr) * | 2008-07-11 | 2010-01-14 | Monash University | Procédé de fabrication de systèmes microfluidiques |
WO2010022324A2 (fr) * | 2008-08-22 | 2010-02-25 | President And Fellows Of Harvard College | Procédés de création de motifs sur du papier |
WO2010102294A1 (fr) | 2009-03-06 | 2010-09-10 | President And Fellows Of Harvard College | Méthodes de micro-impression de microfluides à base de papier |
US20110105360A1 (en) * | 2008-03-27 | 2011-05-05 | President And Fellows Of Harvard College | Paper-based cellular arrays |
WO2011073519A1 (fr) * | 2009-12-15 | 2011-06-23 | Teknologian Tutkimuskeskus Vtt | Procédé de fabrication de structures de guidage de flux de liquide vers des substrats poreux |
WO2011097677A1 (fr) * | 2010-02-12 | 2011-08-18 | Monash University | Plaques à multiples microzones imprimées |
-
2011
- 2011-11-01 EP EP11187407.9A patent/EP2589435A1/fr not_active Withdrawn
-
2012
- 2012-10-31 WO PCT/IB2012/056060 patent/WO2013065000A1/fr active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090298191A1 (en) * | 2006-10-18 | 2009-12-03 | President And Fellows Of Harvard College | Lateral Flow and Flow-through Bioassay Devices Based On Patterned Porous Media, Methods of Making Same, and Methods of Using Same |
US20110105360A1 (en) * | 2008-03-27 | 2011-05-05 | President And Fellows Of Harvard College | Paper-based cellular arrays |
WO2010003188A1 (fr) * | 2008-07-11 | 2010-01-14 | Monash University | Procédé de fabrication de systèmes microfluidiques |
WO2010022324A2 (fr) * | 2008-08-22 | 2010-02-25 | President And Fellows Of Harvard College | Procédés de création de motifs sur du papier |
WO2010102294A1 (fr) | 2009-03-06 | 2010-09-10 | President And Fellows Of Harvard College | Méthodes de micro-impression de microfluides à base de papier |
WO2011073519A1 (fr) * | 2009-12-15 | 2011-06-23 | Teknologian Tutkimuskeskus Vtt | Procédé de fabrication de structures de guidage de flux de liquide vers des substrats poreux |
WO2011097677A1 (fr) * | 2010-02-12 | 2011-08-18 | Monash University | Plaques à multiples microzones imprimées |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2962116A4 (fr) * | 2013-02-28 | 2016-08-10 | Ricoh Co Ltd | Dispositif liquide et procédé de fabrication de celui-ci et véhicule de transfert de chaleur pour la fabrication du dispositif liquide |
EP3697537A4 (fr) * | 2017-10-18 | 2021-10-20 | Group K Diagnostics, Inc. | Dispositif microfluidique monocouche et ses procédés de fabrication et d'utilisation |
US11642669B2 (en) | 2017-10-18 | 2023-05-09 | Group K Diagnostics, Inc. | Single-layer microfluidic device and methods of manufacture and use thereof |
EP4145137A4 (fr) * | 2020-04-28 | 2024-04-24 | Dexerials Corporation | Puce de test et son procédé de fabrication |
CN112973813A (zh) * | 2021-02-10 | 2021-06-18 | 齐鲁工业大学 | 一种用于分离富集外泌体的微流控芯片及其制作方法 |
CN112973813B (zh) * | 2021-02-10 | 2023-03-14 | 齐鲁工业大学 | 一种用于分离富集外泌体的微流控芯片及其制作方法 |
Also Published As
Publication number | Publication date |
---|---|
WO2013065000A1 (fr) | 2013-05-10 |
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