WO2013062383A1 - 세포배양 어세이 - Google Patents
세포배양 어세이 Download PDFInfo
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- WO2013062383A1 WO2013062383A1 PCT/KR2012/008917 KR2012008917W WO2013062383A1 WO 2013062383 A1 WO2013062383 A1 WO 2013062383A1 KR 2012008917 W KR2012008917 W KR 2012008917W WO 2013062383 A1 WO2013062383 A1 WO 2013062383A1
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- scaffold
- channel
- cell culture
- culture assay
- microfluidic channel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
Definitions
- the present invention relates to a cell culture assay, and more particularly, to a cell culture assay having a structure that can be easily mass-produced and more accurately observe the culture process of the cells.
- Cell migration refers to the movement of organisms or individual cells by various physical, chemical, and biological stimuli, and infections such as AIDS, germs, and bacteria, arteriosclerosis, arthritis, periodontitis, psoriasis, cancer, and multiple It is deeply linked to various diseases and biological phenomena in the human body, including sclerosis, male infertility, asbestos poisoning, and ozone poisoning.
- ECM extracellular matrix
- the microfluidic technology can give a microenvironment around the cell, enable real-time observation and accurate quantification of the cell, reduce the amount of cells or samples used, and evaluate various experimental conditions. There is this.
- the technique of integrating the scaffold can induce the cells in three dimensions, and can culture the cells using various directions, such as the inside and both sides of the scaffold, it is possible to culture a variety of cells at once, It is possible to study the interaction of cells and to study the interaction between the cells and the scaffold itself, which can be utilized to develop medical materials. Furthermore, the effects of various materials on cells, including nanomaterials, drugs, and proteins, can be evaluated in three dimensions.
- the microfluidic platform according to the prior art requires a pillar array having a size of several tens to several hundreds of micrometers for fixing between channels of the scaffold, and if there is no pillar, the scaffold may leak into the channel and thus cannot be utilized.
- the scaffold is hardened after being injected into a specific position in the channel, mostly in the form of a liquid, and a pillar is needed to lock the injected scaffold to a specific position before hardening.
- the microfluidic platform described above has a limitation in the area in which the cells react due to the pillar preventing the scaffold from leaking, and there is a serious problem in mass production.
- the pillars continue to be seen during the observation process, which interferes with quantification, and that cells preferentially react with the pillars rather than the scaffolds.
- an object of the present invention is to solve the problems of the prior art as described above, a cell culture assay having a structure in which cells do not preferentially react with the scaffold and at the same time can be fixed between the scaffold channels. It is to provide.
- the present invention is a substrate; A scaffold channel formed along an inner center of the substrate, at least one of which is disposed continuously, and in which a scaffold flows; And microfluidic channels respectively formed on both sides or one side of the scaffold channel, and inside which cells flow, and at least one of the ceiling surface and the bottom surface of the boundary between the microfluidic channel and the scaffold channel is the scaffold. It is characterized in that the leakage preventing portion is formed to prevent the folds from leaking into the microfluidic channel.
- the upper portion of the microfluidic channel and the scaffold channel is formed in a semicircular shape, the upper portion of the semicircular shape is in contact with each other is characterized in that the leakage preventing portion is formed in the boundary portion.
- the leak prevention part is characterized in that the front end is pointed and has a cross section having a curved shape on both sides.
- the leak prevention portion is characterized in that the front end is formed round.
- the leakage preventing portion is characterized in that the front end is formed to have a rectangular cross section.
- the leakage preventing part is formed to occupy 5 to 95% of the height of the scaffold channel and the microfluidic channel.
- the scaffold channel and the microfluidic channel are alternately arranged alternately with each other.
- Both ends of the microfluidic channel extend in a direction away from the scaffold channel.
- the leakage preventing portion is extended to the ceiling surface or the bottom surface of the boundary between the scaffold channel and the microfluidic channel, so that the cells do not react first with other structures, thereby enabling accurate quantification during observation and reaction of the cell culture.
- research on cell interactions is also possible.
- FIG. 1 is a plan view showing a cell culture assay according to an embodiment of the present invention.
- FIG. 2 is a cross-sectional view taken along the line I-I of FIG.
- Figures 3a and 3b is an illustration showing that the scaffold is injected into the cell culture assay according to an embodiment of the present invention to harden.
- FIG 4 and 5 are cross-sectional view of another example of the channel shape of the cell culture assay according to the present invention.
- 6a to 6c is a perspective view showing a process in which the scaffold is injected into the cell culture assay according to an embodiment of the present invention.
- FIG. 7A and 7B are cross-sectional perspective views showing another example of the channel structure of the cell culture assay according to the present invention.
- Figure 8 is a photograph showing the state of cell culture in the conventional cell culture assay.
- Figure 9 is a photograph showing the state of cell culture in the cell culture assay according to the present invention.
- FIG. 1 is a plan view showing a cell culture assay according to an embodiment of the present invention
- Figure 2 is a cross-sectional view taken along the line I-I of FIG.
- the scaffold channel 20 and the microfluidic channel 30 are formed on the substantially rectangular parallelepiped substrate 10, respectively.
- the substrate 10 may be made of a material capable of realizing a microfluidic structure capable of transporting a small flow rate of any material.
- the microfluidic channel 30 into which cells are introduced is located at both sides of the substrate 10, and the scaffold channel 20 is disposed therebetween. As will be described below, a plurality of scaffold channels 20 may be continuously arranged between the microfluidic channels 30.
- the scaffold channel 20 is installed in a straight line across the center of the substrate 10.
- a scaffold inlet 22 is formed at the front end (upstream) of the scaffold channel 20, and a scaffold outlet 24 is formed at the rear end (downstream), respectively.
- the microfluidic inlet 32 and the microfluidic outlet 24 are formed at the front end and the rear end of the microfluidic channel 30, respectively.
- the scaffold channel 20 flows through the scaffold channel 20.
- the scaffold 50 which forms the extracellular matrix (ECM), maintains all the advantages of the microfluidic technology.
- ECM extracellular matrix
- the three-dimensional response of the cells can be simulated during migration assessment.
- This scaffold 50 should flow inside the scaffold channel 20 and should not leak towards the microfluidic channel 30.
- the scaffold 50 hardens after being injected into a specific position within the scaffold channel 20 in liquid form.
- the scaffold 50 may be made of a material which is injected in a fluid form to solidify or gelize, to harden, to increase viscosity, or to form a piper.
- solidifying the scaffold 50 there are methods such as applying heat, chemically mixing, watching for a predetermined time or applying light (especially ultraviolet rays).
- various materials such as alginate, collagen, peptide, fibrin, hyaluronic acid, agarose, and PEG may be used.
- general gel, agarose gel, tissue, protein can be utilized in various matrix materials.
- the scaffold 50 may be made of a material extracted directly from a living body such as cells, cell masses, biological tissues, or a mixture thereof.
- the scaffold channel 20 The leakage preventing part 40 is placed at the boundary where the microfluidic channel 30 meets the gap. That is, the leak prevention portion 40 is formed to extend by a predetermined length on the ceiling surface or the bottom surface of the boundary between the scaffold channel 20 and the microfluidic channel 30 by removing the conventional pillar array.
- FIGS. 3A and 3B the process of injecting the scaffold 50 in a state where the leakage preventing part 40 is formed is well illustrated in FIGS. 3A and 3B.
- both sides may have a concave shape inward due to the cohesive force of the scaffold 50 itself as shown in FIG.
- the fold 50 may be open to both sides and have a convex shape as shown in FIG. 3B. This shape depends on the surface contact angle of the channel and can be manipulated in a particular form.
- the scaffold 50 may be prevented from leaking into the microfluidic channel 30 by the surface tension with the leak prevention part 40.
- the leakage preventing part 40 may be formed on the ceiling surface or the bottom surface of the boundary between the scaffold channel 20 and the microfluidic channel 30 as described above. In FIG. . Most preferably, as shown in FIG. 2, the leakage preventing part 40 has a cross section of which both sides are curved while the tip is sharp. The upper part of the scaffold channel 20 and the microfluidic channel 30 may be formed in a semicircular shape and arranged to contact each other.
- the tip may not be sharply formed as shown in FIG. 2 but may be rounded as shown in FIG. 4.
- the leakage preventing portion 40 may be formed to have a rectangular cross section.
- the leak prevention part 40 described above should be extended by a predetermined length from the ceiling surface or the bottom surface of the boundary between the scaffold channel 20 and the microfluidic channel 30, the shape is not particularly limited. .
- the leakage preventing part 40 may be formed to occupy 5 to 95% of the height of the scaffold channel 20 and the microfluidic channel 30. At this time, if the leak-proof portion 40 is formed to less than 5% of the height of the scaffold channel 20 and the microfluidic channel 30, capillarity is difficult to maintain, if more than 90% drug / molecule The smooth delivery of can be a difficult problem.
- the leak prevention part 40 may be formed under various ranges such as 20 to 80%, 30 to 70%, and 40 to 60% of the height of the scaffold channel 20 and the microfluidic channel 30.
- the microfluidic channel 30 extends in various directions or in a direction away from the scaffold channel 20 as shown in Fig. 1, respectively.
- substantially the scaffold 50 and fluid meet between upstream and downstream.
- the cells may be cultured using various directions such as inside and both sides of the scaffold 50 channel, and various cells may be cultured at once.
- it is possible to study the interaction of the cells using this, and also to study the interaction between the cells and the scaffold itself can be utilized in the development of medical materials.
- Figures 6a to 6c is a perspective view showing a process in which the scaffold is injected into the cell culture assay according to an embodiment of the present invention.
- the scaffold 50 is introduced through the scaffold inlet 22 and flows along the scaffold channel 20.
- the scaffold 50 may be fixed on the scaffold channel 20 without being leaked toward the microfluidic channel 30 by the leak prevention part 40 even though the scaffold 50 is introduced as shown in FIG. 6C.
- Figures 7a and 7b is a cross-sectional perspective view showing another example of the channel structure of the cell culture assay according to the present invention.
- the scaffold channel 20 and microfluidic channel 30 of the cell culture assay may be arranged in various forms.
- the microfluidic channel 30 is disposed on both sides, and one scaffold channel 20 is disposed therebetween.
- the arrangement of the scaffold channel 20 and the microfluidic channel 30 is not necessarily limited to the above-described embodiment, and a plurality of scaffold channels 20 may be continuously arranged as shown in FIG. 4. As in 5, the scaffold channel 20 and the microfluidic channel 30 may be alternately arranged alternately. Such a variety of arrangements have the advantage of allowing simultaneous identification of various cell cultures.
- Figure 8 shows the culture of vascular endothelial cells through a conventional cell culture assay. Looking at the portion shown in yellow in Figure 8, it can be seen that conventionally endothelial cells grow on the column array and the wall surface. That is, there is a limit in the area where the cells react, and there is a problem in that the pillars are seen during the observation process, thus preventing quantification and the cells reacting with the column array first. In addition, the area marked in red should be wider to see the interaction between cells and cells, cells and gels, and chemical factors, but it is difficult to make them wider to maintain surface tension.
- vascular endothelial cells grow stably. Therefore, according to the cell culture assay according to the present invention, it is easy to observe the interaction caused by the cells and the cells, the cells and the gel, and chemical factors, and there is an advantage that the production of the substrate is easy.
- the cell culture assay according to the present invention can be used for various cells including vascular endothelial cells.
- the cell culture assay described above is generally used for evaluation of cell migration under various environmental conditions.
- new drug development development of disease models such as cancer and Alzheimer's disease, construction of tissue and organ models, simulation of biological environment, toxicity evaluation, It can be used for various purposes such as drug evaluation, pollutant evaluation, protein and other substance evaluation, biocompatibility evaluation, and stem cell research.
Abstract
Description
Claims (10)
- 기판;상기 기판의 내부 중앙을 따라 형성되고, 적어도 하나 이상이 연속적으로 배치되며, 내부에는 스캐폴드가 유동하는 스캐폴드 채널; 및상기 스캐폴드 채널의 양측 또는 일측에 각각 형성되고, 내부에는 세포가 유동하는 미세유체 채널을 포함하고,상기 미세유체 채널 및 스캐폴드 채널의 경계부의 천장면 및 바닥면 중 적어도 어느 하나에는 상기 스캐폴드가 미세유체 채널로 누출되는 것을 방지하기 위한 누출방지부가 형성되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 미세유체 채널 및 스캐폴드 채널의 상부는 반원형으로 이루어지고, 반원형의 상부는 서로 접하여 경계부에 상기 누출방지부가 형성되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 누출방지부는 선단이 뾰족하고 양측이 곡면형상을 가진 단면을 가지는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 누출방지부는 선단이 라운드지게 형성되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 누출방지부는 선단이 사각형상의 단면을 가지도록 형성되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 누출방지부는 상기 스캐폴드 채널 및 미세유체 채널의 높이의 5 내지 95%를 차지하도록 형성되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 스캐폴드 채널 및 미세유체 채널은 서로 교대로 반복하여 배치되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 미세유체 채널의 양단부는 상기 스캐폴드 채널에서 멀어지는 방향으로 연장되는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 스캐폴드는 유체 형태로 주입되어 젤화되거나, 경화되는 물질로 이루어지는 것을 특징으로 하는 세포배양 어세이.
- 제 1 항에 있어서,상기 스캐폴드는 알지네이트(Alginate), 콜라겐(Collagen), 펩타이드(Peptide), 피브린(Fibrin), 히알루론산(Hyaluronic Acid), 아가로즈(Agarose), PHEMA(Polyhydroxyethylmethacrylate), PVA(Polyvinyl alcohol), PEG(Poly(ethylene glycol)), PEO(Poly(ethylene oxide)), PEGDA(Polyethylene (glycol) diacrylate), 젤라틴(Gelatin), 매트리젤(Matrigel), PLLA(poly(L-lactic acid)), 카복시메틸셀룰로오스(Carboxymethylcellulose), SAP, PHEMA-MMA, 덱스트란(Dextran), 키토산(Chitosan) 중 어느 하나의 재질 또는, 이들 중 둘 이상이 혼합된 재질로 이루어지는 것을 특징으로 하는 세포배양 어세이.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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US14/354,423 US9523672B2 (en) | 2011-10-28 | 2012-10-29 | Cell culture assay |
EP12842801.8A EP2772530B1 (en) | 2011-10-28 | 2012-10-29 | Cell culture assay |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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KR10-2011-0111302 | 2011-10-28 | ||
KR1020110111302A KR101322798B1 (ko) | 2011-10-28 | 2011-10-28 | 세포배양 어세이 |
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WO2013062383A1 true WO2013062383A1 (ko) | 2013-05-02 |
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US (1) | US9523672B2 (ko) |
EP (1) | EP2772530B1 (ko) |
JP (2) | JP2014530635A (ko) |
KR (1) | KR101322798B1 (ko) |
WO (1) | WO2013062383A1 (ko) |
Families Citing this family (14)
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KR101322798B1 (ko) * | 2011-10-28 | 2013-10-29 | 고려대학교 산학협력단 | 세포배양 어세이 |
CN105713835B (zh) * | 2014-12-05 | 2018-11-09 | 中国科学院大连化学物理研究所 | 一种基于微流控芯片的多功能区域细胞三维共培养方法 |
KR101896618B1 (ko) * | 2015-12-07 | 2018-09-07 | 서울대학교산학협력단 | 세포외소포체 추적 관찰 시스템 |
KR20170074503A (ko) * | 2015-12-22 | 2017-06-30 | 고려대학교 산학협력단 | 세포 배양 어세이를 이용한 약물 평가 방법 |
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