JP6457434B2 - 細胞培養アッセイ - Google Patents
細胞培養アッセイ Download PDFInfo
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- JP6457434B2 JP6457434B2 JP2016104036A JP2016104036A JP6457434B2 JP 6457434 B2 JP6457434 B2 JP 6457434B2 JP 2016104036 A JP2016104036 A JP 2016104036A JP 2016104036 A JP2016104036 A JP 2016104036A JP 6457434 B2 JP6457434 B2 JP 6457434B2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
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- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
20 スキャフォルドチャネル
22 スキャフォルド流入口
24 スキャフォルド流出口
30 微細流体チャネル
32 微細流体流入口
34 微細流体流出口
40 漏出防止部
50 スキャフォルド
Claims (10)
- 基板と;
前記基板の内部中央に沿って形成され、少なくとも1つ以上が連続的に配置され、内部にはスキャフォルドが流動するスキャフォルドチャネルと;
前記スキャフォルドチャネルの両側または一側にそれぞれ形成され、内部には細胞が流動する微細流体チャネルと;を含み、
前記微細流体チャネル及びスキャフォルドチャネルの境界部の天井面には、前記スキャフォルドが微細流体チャネルに漏出されることを防止するための漏出防止部が形成され、
前記漏出防止部は、前記スキャフォルドチャネル及び微細流体チャネルの高さの20〜80%を占めるように構成され、
前記漏出防止部は、前記スキャフォルドチャネルと前記微細流体チャネルとが接する長手方向の境界部に連続的に設けられることを特徴とする、細胞培養アッセイデバイス。 - 前記微細流体チャネル及びスキャフォルドチャネルの上部は、半円形よりなり、半円形の上部は、互いに当接し、境界部に前記漏出防止部が形成されることを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記漏出防止部は、先端がとがっていて、両側が曲面形状を有する断面を有することを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記漏出防止部は、先端がラウンド状に形成されることを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記漏出防止部は、先端が四角形状の断面を有するように形成されることを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記スキャフォルドチャネル及び微細流体チャネルは、互いに交互に繰り返して配置されることを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記微細流体チャネルの両端部は、前記スキャフォルドチャネルから離れる方向に延長することを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記スキャフォルドは、流体形態で注入されてゲル化するか、または硬化する物質よりなることを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記スキャフォルドは、アルジネート(Alginate)、コラーゲン(Collagen)、ペプチド(Peptide)、フィブリン(Fibrin)、ヒアルロン酸(Hyaluronic Acid)、アガロース(Agarose)、PHEMA(Polyhydroxyethylmethacrylate)、PVA(Polyvinylalcohol)、PEG(Poly(ethylene glycol))、PEO(Poly(ethyleneoxide))、PEGDA(Polyethylene(glycol) diacrylate)、ゼラチン(Gelatin)、マトリゲル(Matrigel)、PLLA(poly(L−lactic acid))、カルボキシメチルセルロース(Carboxymethylcellulose)、SAP、PHEMA−MMA、デキストラン(Dextran)、キトサン(Chitosan)のうちいずれか1つの材質または、これらのうち2つ以上が混合された材質よりなることを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
- 前記細胞培養アッセイデバイスは、スキャフォルドチャネル間に固定するための柱アレイを含まないことを特徴とする、請求項1に記載の細胞培養アッセイデバイス。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2011-0111302 | 2011-10-28 | ||
KR1020110111302A KR101322798B1 (ko) | 2011-10-28 | 2011-10-28 | 세포배양 어세이 |
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JP2014538717A Division JP2014530635A (ja) | 2011-10-28 | 2012-10-29 | 細胞培養アッセイ |
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JP2016146851A JP2016146851A (ja) | 2016-08-18 |
JP6457434B2 true JP6457434B2 (ja) | 2019-01-23 |
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JP2014538717A Pending JP2014530635A (ja) | 2011-10-28 | 2012-10-29 | 細胞培養アッセイ |
JP2016104036A Active JP6457434B2 (ja) | 2011-10-28 | 2016-05-25 | 細胞培養アッセイ |
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JP2014538717A Pending JP2014530635A (ja) | 2011-10-28 | 2012-10-29 | 細胞培養アッセイ |
Country Status (5)
Country | Link |
---|---|
US (1) | US9523672B2 (ja) |
EP (1) | EP2772530B1 (ja) |
JP (2) | JP2014530635A (ja) |
KR (1) | KR101322798B1 (ja) |
WO (1) | WO2013062383A1 (ja) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101322798B1 (ko) * | 2011-10-28 | 2013-10-29 | 고려대학교 산학협력단 | 세포배양 어세이 |
CN105713835B (zh) * | 2014-12-05 | 2018-11-09 | 中国科学院大连化学物理研究所 | 一种基于微流控芯片的多功能区域细胞三维共培养方法 |
KR101896618B1 (ko) * | 2015-12-07 | 2018-09-07 | 서울대학교산학협력단 | 세포외소포체 추적 관찰 시스템 |
KR20170074503A (ko) * | 2015-12-22 | 2017-06-30 | 고려대학교 산학협력단 | 세포 배양 어세이를 이용한 약물 평가 방법 |
WO2017218581A1 (en) * | 2016-06-13 | 2017-12-21 | Massachusetts Institute Of Technology | Microfluidic device for three dimensional and compartmentalized coculture of neuronal and muscle cells, with functional force readout |
WO2018063099A1 (en) | 2016-09-29 | 2018-04-05 | Nanyang Technological University | Three-dimensional (3d) hydrogel patterning in microfluidic vascular models |
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