JP2014530635A - 細胞培養アッセイ - Google Patents
細胞培養アッセイ Download PDFInfo
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- JP2014530635A JP2014530635A JP2014538717A JP2014538717A JP2014530635A JP 2014530635 A JP2014530635 A JP 2014530635A JP 2014538717 A JP2014538717 A JP 2014538717A JP 2014538717 A JP2014538717 A JP 2014538717A JP 2014530635 A JP2014530635 A JP 2014530635A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M23/16—Microfluidic devices; Capillary tubes
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- C12M23/34—Internal compartments or partitions
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- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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Abstract
Description
20 スキャフォルドチャネル
22 スキャフォルド流入口
24 スキャフォルド流出口
30 微細流体チャネル
32 微細流体流入口
34 微細流体流出口
40 漏出防止部
50 スキャフォルド
Claims (10)
- 基板と;
前記基板の内部中央に沿って形成され、少なくとも1つ以上が連続的に配置され、内部にはスキャフォルドが流動するスキャフォルドチャネルと;
前記スキャフォルドチャネルの両側または一側にそれぞれ形成され、内部には細胞が流動する微細流体チャネルと;を含み、
前記微細流体チャネル及びスキャフォルドチャネルの境界部の天井面及び底面のうち少なくともいずれか1つには、前記スキャフォルドが微細流体チャネルに漏出されることを防止するための漏出防止部が形成されることを特徴とする、細胞培養アッセイ。 - 前記微細流体チャネル及びスキャフォルドチャネルの上部は、半円形よりなり、半円形の上部は、互いに当接し、境界部に前記漏出防止部が形成されることを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記漏出防止部は、先端がとがっていて、両側が曲面形状を有する断面を有することを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記漏出防止部は、先端がラウンド状に形成されることを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記漏出防止部は、先端が四角形状の断面を有するように形成されることを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記漏出防止部は、前記スキャフォルドチャネル及び微細流体チャネルの高さの5〜95%を占めるように形成されることを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記スキャフォルドチャネル及び微細流体チャネルは、互いに交互に繰り返して配置されることを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記微細流体チャネルの両端部は、前記スキャフォルドチャネルから離れる方向に延長することを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記スキャフォルドは、流体形態で注入されてゲル化するか、または硬化する物質よりなることを特徴とする、請求項1に記載の細胞培養アッセイ。
- 前記スキャフォルドは、アルジネート(Alginate)、コラーゲン(Collagen)、ペプチド(Peptide)、フィブリン(Fibrin)、ヒアルロン酸(Hyaluronic Acid)、アガロース(Agarose)、PHEMA(Polyhydroxyethylmethacrylate)、PVA(Polyvinylalcohol)、PEG(Poly(ethylene glycol))、PEO(Poly(ethyleneoxide))、PEGDA(Polyethylene(glycol) diacrylate)、ゼラチン(Gelatin)、マトリゲル(Matrigel)、PLLA(poly(L−lactic acid))、カルボキシメチルセルロース(Carboxymethylcellulose)、SAP、PHEMA−MMA、デキストラン(Dextran)、キトサン(Chitosan)のうちいずれか1つの材質または、これらのうち2つ以上が混合された材質よりなることを特徴とする、請求項1に記載の細胞培養アッセイ。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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KR10-2011-0111302 | 2011-10-28 | ||
KR1020110111302A KR101322798B1 (ko) | 2011-10-28 | 2011-10-28 | 세포배양 어세이 |
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JP2016104036A Division JP6457434B2 (ja) | 2011-10-28 | 2016-05-25 | 細胞培養アッセイ |
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JP2014530635A true JP2014530635A (ja) | 2014-11-20 |
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JP2014538717A Pending JP2014530635A (ja) | 2011-10-28 | 2012-10-29 | 細胞培養アッセイ |
JP2016104036A Active JP6457434B2 (ja) | 2011-10-28 | 2016-05-25 | 細胞培養アッセイ |
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JP2016104036A Active JP6457434B2 (ja) | 2011-10-28 | 2016-05-25 | 細胞培養アッセイ |
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US (1) | US9523672B2 (ja) |
EP (1) | EP2772530B1 (ja) |
JP (2) | JP2014530635A (ja) |
KR (1) | KR101322798B1 (ja) |
WO (1) | WO2013062383A1 (ja) |
Cited By (3)
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JP2016146851A (ja) * | 2011-10-28 | 2016-08-18 | コリア・ユニバーシティ・リサーチ・アンド・ビジネス・ファウンデーション | 細胞培養アッセイ |
JP2019531731A (ja) * | 2016-09-29 | 2019-11-07 | ナンヤン テクノロジカル ユニヴァーシティー | マイクロ流体血管モデルにおける三次元(3d)ハイドロゲルパターニング |
WO2023145208A1 (ja) * | 2022-01-31 | 2023-08-03 | 株式会社エンプラス | 流体取扱装置、その製造方法、および流体取扱装置への流動性媒体の導入方法 |
Families Citing this family (11)
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CN105713835B (zh) * | 2014-12-05 | 2018-11-09 | 中国科学院大连化学物理研究所 | 一种基于微流控芯片的多功能区域细胞三维共培养方法 |
KR101896618B1 (ko) * | 2015-12-07 | 2018-09-07 | 서울대학교산학협력단 | 세포외소포체 추적 관찰 시스템 |
KR20170074503A (ko) * | 2015-12-22 | 2017-06-30 | 고려대학교 산학협력단 | 세포 배양 어세이를 이용한 약물 평가 방법 |
WO2017218581A1 (en) * | 2016-06-13 | 2017-12-21 | Massachusetts Institute Of Technology | Microfluidic device for three dimensional and compartmentalized coculture of neuronal and muscle cells, with functional force readout |
KR101949612B1 (ko) * | 2016-11-29 | 2019-02-19 | 고려대학교 산학협력단 | 미세 유체칩 |
IT201600131735A1 (it) * | 2016-12-28 | 2018-06-28 | Milano Politecnico | Costrutti tridimensionali multistrato in microcanali |
KR101848201B1 (ko) * | 2017-01-04 | 2018-05-24 | 고려대학교 산학협력단 | 조직을 구성하는 이기종 세포 간의 반응 연구를 위한 반응 연구 칩 |
EP3642352A4 (en) * | 2017-06-19 | 2021-03-24 | Curiochips | MICROFLUIDIC DEVICE WITH PARTIALLY ENCAPSULATED MICROFLUIDIC CHANNEL AND THE USE OF IT |
KR20190088354A (ko) | 2018-01-18 | 2019-07-26 | 포항공과대학교 산학협력단 | 세포친화성 물질로 피복된 나노 섬유상 기저 막과 제조 방법 |
KR20190088337A (ko) | 2018-01-18 | 2019-07-26 | 포항공과대학교 산학협력단 | 나노 섬유 기저 막을 생체 모사 조직 표면에 형성시키는 방법 |
CA3180861A1 (en) * | 2020-04-22 | 2021-10-28 | The Board Of Trustees Of The Leland Stanford Junior University | Microfluidic chips and microphysiological systems using the same |
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2012
- 2012-10-29 WO PCT/KR2012/008917 patent/WO2013062383A1/ko active Application Filing
- 2012-10-29 EP EP12842801.8A patent/EP2772530B1/en active Active
- 2012-10-29 US US14/354,423 patent/US9523672B2/en active Active
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Cited By (5)
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JP2016146851A (ja) * | 2011-10-28 | 2016-08-18 | コリア・ユニバーシティ・リサーチ・アンド・ビジネス・ファウンデーション | 細胞培養アッセイ |
JP2019531731A (ja) * | 2016-09-29 | 2019-11-07 | ナンヤン テクノロジカル ユニヴァーシティー | マイクロ流体血管モデルにおける三次元(3d)ハイドロゲルパターニング |
JP7076820B2 (ja) | 2016-09-29 | 2022-05-30 | ナンヤン テクノロジカル ユニヴァーシティー | マイクロ流体血管モデルにおける三次元(3d)ハイドロゲルパターニングのためのデバイス及び方法 |
US11794184B2 (en) | 2016-09-29 | 2023-10-24 | Nanyang Technological University | Three-dimensional (3D) hydrogel patterning in microfluidic vascular models |
WO2023145208A1 (ja) * | 2022-01-31 | 2023-08-03 | 株式会社エンプラス | 流体取扱装置、その製造方法、および流体取扱装置への流動性媒体の導入方法 |
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JP6457434B2 (ja) | 2019-01-23 |
KR101322798B1 (ko) | 2013-10-29 |
KR20130046750A (ko) | 2013-05-08 |
US9523672B2 (en) | 2016-12-20 |
WO2013062383A1 (ko) | 2013-05-02 |
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JP2016146851A (ja) | 2016-08-18 |
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