WO2013053071A1 - Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose - Google Patents

Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose Download PDF

Info

Publication number
WO2013053071A1
WO2013053071A1 PCT/CN2011/001686 CN2011001686W WO2013053071A1 WO 2013053071 A1 WO2013053071 A1 WO 2013053071A1 CN 2011001686 W CN2011001686 W CN 2011001686W WO 2013053071 A1 WO2013053071 A1 WO 2013053071A1
Authority
WO
WIPO (PCT)
Prior art keywords
sugar
gum
hemicellulose
mannose
mannan
Prior art date
Application number
PCT/CN2011/001686
Other languages
English (en)
Chinese (zh)
Inventor
黄代勇
钱金宏
Original Assignee
Huang Daiyong
Qian Jimhowell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huang Daiyong, Qian Jimhowell filed Critical Huang Daiyong
Priority to PCT/CN2011/001686 priority Critical patent/WO2013053071A1/fr
Publication of WO2013053071A1 publication Critical patent/WO2013053071A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0057—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Xylans, i.e. xylosaccharide, e.g. arabinoxylan, arabinofuronan, pentosans; (beta-1,3)(beta-1,4)-D-Xylans, e.g. rhodymenans; Hemicellulose; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase

Definitions

  • the invention relates to an enzyme technology and a biochemical technology, and more specifically to a method and a product for preparing a mannan oligosaccharide having a purity of up to 98% (P98) by enzymatic hydrolysis of a mannan-containing hemicellulose, which is a gastrointestinal health , immune regulators, new raw materials and additives for disease-resistant and disease-preventing drugs.
  • P98 mannan oligosaccharide having a purity of up to 98%
  • Hemi cellulose refers to the part of plant polysaccharide that is symbiotic with cellulose in the cell wall of plants, soluble in alkaline solution, and is much easier to hydrolyze than cellulose after acid. Hemicellulose is mainly classified into three types, namely, polyxylose, polyglucomannan, and polygalactose grape mannose.
  • the hemicelluloses to which the present invention relates are polyglucomannans (representing products are konjac gum) and polygalactomannoses (representing products are guar gum), collectively referred to as mannans.
  • the mannan in hemicellulose is enzymatically hydrolyzed to form a low carbon chain mannose heteropolymer.
  • low-carbon chain mannose heteropolymers also known as mannose oligosaccharides or mannooligosaccharides
  • Recent reports are: Huang Daiyong et al.
  • the enzyme activity is not high, the substrate concentration is low, the cost is high, and the basis of industrial production is lacking; or the alkali and acid are added during the hydrolysis process. It brings difficulties to the post-treatment process and is not conducive to environmental protection; or the purity of the product is low, generally only 70 ⁇ 80%.
  • the purity of konjac mannose oligosaccharide has reached 95%, but its sugar molecule polymerization degree is as large as 3 ⁇ 10 sugar. Due to the limitation of function, it can only be applied to food and chemical products. There is still a certain gap to be applied to pharmaceutical products.
  • the object of the present invention is to provide a high-efficiency e-mannan S technology and food production produced by fermentation of Bacillus subtilis TQBm with the preservation number CCTCC No : M 211147 using hemicellulose containing mannan as raw material.
  • the system and technology of fine chemical technology integrate the production method and product of mannose oligosaccharide with purity of up to 98% (P98) and molecular polymerization degree of 4 ⁇ 8 sugar.
  • the product can be used as gastrointestinal health, immune regulation and disease resistance. New raw materials and additives for disease drugs.
  • the present invention is achieved in such a manner that the process is:
  • the invention adopts the high-efficiency e-mannanase technology produced by the fermentation of Bacillus subtilis TQBm with the accession number CCTCC No : M 211147, and the system integration of food production technology and fine chemical technology is prepared from konjac gum and guar gum.
  • the P98 mannose oligosaccharide has simple process, low energy consumption, convenient operation, high product purity, controllable cost and no chemical pollution.
  • the hemicellulose of the mannan used in the present invention includes konjac gum, guar gum, locust bean gum, celery gum, acacia gum, fenugreek gum and the like.
  • the product produced by the invention has high purity, and the functional ingredient mannose 4 sugar ⁇ mannose 8 sugar accounts for a large proportion, and has the advantage of being uniquely applied to medical products in the same kind of products, and will greatly inspire people to expand the high-end application range of oligosaccharides. New ideas, new creations, new prospects. detailed description
  • Preheating The enzymatic tank jacket is steamed or hot water to preheat the water in the tank to 50 ⁇ 52 ⁇ .
  • Enzymatic hydrolysis The temperature in the tank is kept between 50 ⁇ 55 °C, stirring constantly, and enzymatic hydrolysis for 3 ⁇ 4 hours. In the enzymatic hydrolysis process, the viscosity of the enzymatic hydrolysate at 50 Torr was measured with a NDJ-1 type rotational viscometer at 12 rpm. When the viscosity of the enzymatic hydrolysate was 80 to 100 mPa*s, the degradation was stopped. The liquid was called “enzyme.” Dissolve stock solution”.
  • the ultrafiltration membrane with a pore size of 30,000 molecular weight is used for ultrafiltration purification.
  • the working conditions are: temperature 25 ⁇ 35'C, pressure 0.1 ⁇ 0.12 Mpa, material flow rate 300 ⁇ 350I7h.
  • the ion exchange purification solution was concentrated in a vacuum and spray-dried to obtain 58.3 kg of P98 mannan, and the yield of the sugar powder was 41.64% (relative to konjac gum).
  • Liquid chromatography-mass spectrometry was used to detect P98 konjac mannose oligosaccharides, total sugar: ⁇ 99.5%, efficacy component (mannose 2 sugar ⁇ mannose 10 sugar) 98.0%, of which mannose 4 sugar ⁇ nectar 8 sugar accounted for more than 85% .
  • Preheating The enzymatic tank jacket is steamed or hot water to preheat the water in the tank to 50 ⁇ 52 °C.
  • Feeding Weigh 105kg of guar gum into the enzymatic hydrolysis tank (substrate concentration 15%), and stir well.
  • Enzymatic hydrolysis The temperature in the tank is kept between 50 and 55 ° C, and the mixture is continuously stirred and digested for 3 to 4 hours. In the enzymatic hydrolysis process, the viscosity of the enzymatic hydrolysate was measured at a rotation speed of 12 rpm using an NDJ-1 type rotational viscometer. When the viscosity of the enzymatic hydrolyzate was 80 to 100 mP a , s, the enzymatic hydrolysis was stopped. "Enzymatic hydrolysate stock solution”.
  • the finished P98 mannose oligosaccharide was prepared 47. 72kg, and the sugar powder yield was 45.45% (relative to guar gum). Determination of P98 guar gum mannose oligosaccharide by liquid chromatography-mass spectrometry, total sugar: 99.5%, efficacy component (mannose 2 sugar ⁇ mannose 10 sugar) 98%, of which mannose 4 sugar ⁇ nectar 8 sugar accounted for 85 %the above.
  • the method for preparing P98 mannose oligosaccharide using the ⁇ -mannanase BM-II was the same as that of Example 2, using locust bean gum, celery gum, acacia gum and fenugreek gum as raw materials.
  • test results of 30 adult volunteers with normal physical examination indicators were as follows: Bacteria Enterobacteriaceae Enterococci Bacteroides Producing a bacterium Abacillus brevis Lactobacillus Content Clostridium
  • HDL-C High Density Lipoprotein Cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Materials Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Sustainable Development (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne un procédé de préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'une hémicellulose comprenant un oligosaccharide mannane, ainsi qu'un produit. En utilisant une β-mannanase de grande efficacité, BM-II, produite par la fermentation d'une souche TQBm de Bacillus subtilis de numéro de dépôt CCTCC no M211147, une gomme de konjac Amorphophallus, une gomme de guar, une gomme de caroube et une gomme Sesbania sont hydrolysées en molécules relativement petites, puis sont séparées par centrifugation, clarifiées par floculation, dessalées, décolorées et désodorisées en utilisant une technologie d'échange d'ions, puis les grandes molécules sont séparées dans une solution de sucre en utilisant en combinaison une technologie de super-filtration et une technologie de chromatographie à tamis moléculaire, et enfin l'oligosaccharide mannane de grande pureté est préparé par concentration et séchage. L'oligosaccharide mannane de grande pureté obtenu par la préparation est économique, exempt de contamination chimique, permet la mise en œuvre d'une production industrialisée, augmente significativement les bactéries bénéfiques telles que les bifidobactéries intestinales humaines, réduit significativement les bactéries nocives telles qu'Enterobacter, et permet également des réductions des sucres sanguins et des lipides sanguins et une immunomodulation, et constitue une matière première et un additif pour un médicament pour la santé des voies gastro-intestinales, pour la réduction des sucres sanguins et des lipides sanguins, pour l'immunomodulation et pour le traitement et la prévention de maladies.
PCT/CN2011/001686 2011-10-10 2011-10-10 Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose WO2013053071A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2011/001686 WO2013053071A1 (fr) 2011-10-10 2011-10-10 Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2011/001686 WO2013053071A1 (fr) 2011-10-10 2011-10-10 Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose

Publications (1)

Publication Number Publication Date
WO2013053071A1 true WO2013053071A1 (fr) 2013-04-18

Family

ID=48081320

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2011/001686 WO2013053071A1 (fr) 2011-10-10 2011-10-10 Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose

Country Status (1)

Country Link
WO (1) WO2013053071A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104232534A (zh) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 一种坚强芽孢杆菌活菌制剂的制备工艺
CN104232530A (zh) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 一种地衣芽孢杆菌活菌制剂的制备工艺
CN104232526A (zh) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 一种枯草芽孢杆菌活菌制剂的制备工艺
CN104818235A (zh) * 2015-05-18 2015-08-05 湖北工业大学 一种路氏肠杆菌及其应用
CN108410922A (zh) * 2017-10-26 2018-08-17 绿麒（厦门）海洋生物科技有限公司 一种高品质刺槐豆胶的制备方法
CN108634102A (zh) * 2018-05-16 2018-10-12 中国农业科学院饲料研究所 一种酶降解半乳甘露聚糖产品及其制备方法与应用
CN111118084A (zh) * 2019-12-27 2020-05-08 苏州司克瑞特生物科技有限公司 魔芋甘露寡糖的制备方法
CN111393536A (zh) * 2020-03-31 2020-07-10 华中农业大学 魔芋葡甘聚糖的降解方法
CN113974202A (zh) * 2021-10-25 2022-01-28 深圳市真味生物科技有限公司 一种环保型电子雾化液增稠剂及其制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003012110A1 (fr) * 2001-07-28 2003-02-13 Midwest Research Institute Mannanase thermo-tolerante provenant d'acidothermus cellulolyticus
CN1614005A (zh) * 2004-11-30 2005-05-11 北京科技大学 一种苏云金芽孢杆菌生物农药易粉碎固态培养基生产工艺
CN101240305A (zh) * 2007-09-06 2008-08-13 武汉东方天琪生物工程有限公司 一种酶法降解壳聚糖生产壳寡糖的方法
CN102373256A (zh) * 2011-10-10 2012-03-14 武汉东方天琪生物工程有限公司 酶解半纤维素制备高纯度甘露低聚糖的生产方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003012110A1 (fr) * 2001-07-28 2003-02-13 Midwest Research Institute Mannanase thermo-tolerante provenant d'acidothermus cellulolyticus
CN1614005A (zh) * 2004-11-30 2005-05-11 北京科技大学 一种苏云金芽孢杆菌生物农药易粉碎固态培养基生产工艺
CN101240305A (zh) * 2007-09-06 2008-08-13 武汉东方天琪生物工程有限公司 一种酶法降解壳聚糖生产壳寡糖的方法
CN102373256A (zh) * 2011-10-10 2012-03-14 武汉东方天琪生物工程有限公司 酶解半纤维素制备高纯度甘露低聚糖的生产方法

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104232534A (zh) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 一种坚强芽孢杆菌活菌制剂的制备工艺
CN104232530A (zh) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 一种地衣芽孢杆菌活菌制剂的制备工艺
CN104232526A (zh) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 一种枯草芽孢杆菌活菌制剂的制备工艺
CN104818235A (zh) * 2015-05-18 2015-08-05 湖北工业大学 一种路氏肠杆菌及其应用
CN108410922A (zh) * 2017-10-26 2018-08-17 绿麒（厦门）海洋生物科技有限公司 一种高品质刺槐豆胶的制备方法
CN108634102A (zh) * 2018-05-16 2018-10-12 中国农业科学院饲料研究所 一种酶降解半乳甘露聚糖产品及其制备方法与应用
CN108634102B (zh) * 2018-05-16 2022-03-15 中国农业科学院饲料研究所 一种酶降解半乳甘露聚糖产品及其制备方法与应用
CN111118084A (zh) * 2019-12-27 2020-05-08 苏州司克瑞特生物科技有限公司 魔芋甘露寡糖的制备方法
CN111393536A (zh) * 2020-03-31 2020-07-10 华中农业大学 魔芋葡甘聚糖的降解方法
CN113974202A (zh) * 2021-10-25 2022-01-28 深圳市真味生物科技有限公司 一种环保型电子雾化液增稠剂及其制备方法
CN113974202B (zh) * 2021-10-25 2023-09-01 深圳市真味生物科技有限公司 一种环保型电子雾化液增稠剂及其制备方法

Similar Documents

Publication Publication Date Title
WO2013053071A1 (fr) Procédé de production pour la préparation d'un oligosaccharide mannane de grande pureté par enzymolyse d'hémicellulose
US7923437B2 (en) Water soluble β-glucan, glucosamine, and N-acetylglucosamine compositions and methods for making the same
US8222232B2 (en) Glucosamine and N-acetylglucosamine compositions and methods of making the same fungal biomass
CN102373256B (zh) 酶解半纤维素制备高纯度甘露低聚糖的生产方法
WO2006011479A1 (fr) Procédés de production d’un cello-oligosaccharide
CN106387923B (zh) 一种富含半乳甘露聚糖的可溶性膳食纤维及其制备方法
US10632136B2 (en) Soluble dietary fiber and preparation method thereof
WO2010020095A1 (fr) Méthode pour la fabrication de fructooligosaccharide hautement purifié
Cardelle-Cobas et al. Bifidogenic effect and stimulation of short chain fatty acid production in human faecal slurry cultures by oligosaccharides derived from lactose and lactulose
CN111285939A (zh) 一种具有抗氧化和调节肠道菌群功效的海蒿子多糖及其制备方法与应用
WO2021081999A1 (fr) Sulfate de chondroïtine de faible poids moléculaire et son procédé de préparation
Gullón et al. Assessment on the fermentability of xylooligosaccharides from rice husks.
CN1884308A (zh) 一种生产壳寡糖的方法
Wang et al. Preparation and gut microbiota modulatory property of the oligosaccharide riclinoctaose
CN110862461B (zh) 一种抗性糊精制备方法
CN101168570B (zh) 海带硫酸多糖的降解方法
WO2023170426A1 (fr) Composition de galacto-oligosaccharide
EP0265970A2 (fr) Composition promouvant la prolifération de lactobacillus bifidus
Ríos-Ríos et al. Production of tailored xylo-oligosaccharides from beechwood xylan by different enzyme membrane reactors and evaluation of their prebiotic activity
CN107828834A (zh) 一种低聚半乳糖的制备方法
KR20020031561A (ko) 인삼 올리고당 및 그 제조방법
JP2003048901A (ja) 長鎖キシロオリゴ糖組成物およびその製造方法
CN110669808A (zh) 一种高蔗果三糖含量的低聚果糖的制备方法
EP4368697A1 (fr) Paroi cellulaire de levure hautement immune, son procédé de préparation et son utilisation
CN105559038B (zh) 一种淀粉基膳食纤维的制备方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11873943

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 06/08/2014)

122 Ep: pct application non-entry in european phase

Ref document number: 11873943

Country of ref document: EP

Kind code of ref document: A1