WO2013050024A3 - Transgene pflanze der art beta vulgaris mit gesteigerter resistenz gegenüber cercospora - Google Patents

Transgene pflanze der art beta vulgaris mit gesteigerter resistenz gegenüber cercospora Download PDF

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Publication number
WO2013050024A3
WO2013050024A3 PCT/DE2012/000974 DE2012000974W WO2013050024A3 WO 2013050024 A3 WO2013050024 A3 WO 2013050024A3 DE 2012000974 W DE2012000974 W DE 2012000974W WO 2013050024 A3 WO2013050024 A3 WO 2013050024A3
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Prior art keywords
plant
fungus
cercospora
beta vulgaris
enhanced resistance
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PCT/DE2012/000974
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English (en)
French (fr)
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WO2013050024A2 (de
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Dietmar Stahl
Nora TEMME
Wilhelm Schäfer
Cornelia STÄRKEL
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Kws Saat Ag
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Priority to DE112012004161.5T priority Critical patent/DE112012004161A5/de
Publication of WO2013050024A2 publication Critical patent/WO2013050024A2/de
Publication of WO2013050024A3 publication Critical patent/WO2013050024A3/de

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

Abstract

Die Erfindung betrifft eine transgene Pflanze der Art Beta vulgaris, in deren Genom eine Nukleinsäure stabil integriert wurde, von der in der Pflanze RNA transkribiert wird, wobei gebildete RNA im Falle eines Befalls der Pflanze mit einem Pilz der Gattung Cercospora von diesem aufgenommen werden kann, so dass die Cercosporin-Biosynthese in dem Pilz derart beeinträchtigt wird, dass der Pilz in seiner biotrophen Wachstumsphase gehemmt wird und die Pflanze im Vergleich zu einer Kontrollpflanze eine gesteigerte Resistenz gegenüber dem Pilz aufweist.
PCT/DE2012/000974 2011-10-06 2012-10-04 Transgene pflanze der art beta vulgaris mit gesteigerter resistenz gegenüber cercospora WO2013050024A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112012004161.5T DE112012004161A5 (de) 2011-10-06 2012-10-04 Transgene Pflanze der Art Beta vulgaris mit gesteigerter Resistenz gegenüber Cercospora

Applications Claiming Priority (2)

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DE102011114914.0 2011-10-06
DE102011114914A DE102011114914A1 (de) 2011-10-06 2011-10-06 Transgene pflanze der art beta vulgaris mit gesteigerter resistenz gegenüber cercospora

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WO2013050024A2 WO2013050024A2 (de) 2013-04-11
WO2013050024A3 true WO2013050024A3 (de) 2013-07-11

Family

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DE (2) DE102011114914A1 (de)
WO (1) WO2013050024A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102013010026A1 (de) 2013-06-17 2014-12-18 Kws Saat Ag Resistenzgen gegen Rizomania
EP3282016A1 (de) 2016-08-10 2018-02-14 Kws Saat Se Resistenzgen gegen wurzelbärtigkeit
EP3567111A1 (de) 2018-05-09 2019-11-13 KWS SAAT SE & Co. KGaA Gen für resistenz gegen ein pathogen der gattung heterodera
AU2019430875A1 (en) 2019-02-18 2021-09-23 KWS SAAT SE & Co. KGaA Gene for resistance to plant disease
EP3696188A1 (de) 2019-02-18 2020-08-19 KWS SAAT SE & Co. KGaA Resistenzgene gegen pflanzenkrankheit
JP2023510457A (ja) 2019-11-12 2023-03-14 カー・ヴェー・エス ザート エス・エー ウント コー． カー・ゲー・アー・アー ヘテロデラ属の病原体に対する耐性遺伝子
EP3957168A1 (de) 2020-08-17 2022-02-23 KWS SAAT SE & Co. KGaA Pflanzenresistenzgen und mittel zu dessen identifizierung

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US20070061918A1 (en) * 2003-12-23 2007-03-15 Rachel Baltz Method for modifying gene expression of a phytopathogenic fungus
WO2009112270A2 (de) * 2008-03-13 2009-09-17 Leibniz-Institut für Pflanzengenetik Und Kulturpflanzenforschung (IPK) Verfahren zur erzeugung einer breitband-resistenz gegenüber pilzen in transgenen pflanzen

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US6077995A (en) 1996-03-21 2000-06-20 The United States Of America As Represented By The Secretary Of Agriculture Fungal gene encoding resistance to the phytotoxin cercosporin
EP1207204A1 (de) 2000-11-16 2002-05-22 KWS Saat AG Gewebespezifische Promotoren aus der Zuckerrübe

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US20070061918A1 (en) * 2003-12-23 2007-03-15 Rachel Baltz Method for modifying gene expression of a phytopathogenic fungus
WO2006070227A2 (en) * 2004-10-04 2006-07-06 Devgen Nv Method for down-regulating gene expression in fungi
WO2006047495A2 (en) * 2004-10-21 2006-05-04 Venganza Inc Methods and materials for conferring resistance to pests and pathogens of plants
WO2006097465A2 (de) * 2005-03-14 2006-09-21 Leibniz-Institut für Pflanzengenetik Und Kulturpflanzenforschung (IPK) Verfahren zur erhöhung der pilzresistenz in transgenen pflanzen durch wirtsinduzierte unterdrückung der genexpression in pilzpathogenen
WO2009112270A2 (de) * 2008-03-13 2009-09-17 Leibniz-Institut für Pflanzengenetik Und Kulturpflanzenforschung (IPK) Verfahren zur erzeugung einer breitband-resistenz gegenüber pilzen in transgenen pflanzen

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DE112012004161A5 (de) 2014-06-26
WO2013050024A2 (de) 2013-04-11

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