WO2014100878A3 - Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes da família da lacase - Google Patents

Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes da família da lacase Download PDF

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Publication number
WO2014100878A3
WO2014100878A3 PCT/BR2013/000607 BR2013000607W WO2014100878A3 WO 2014100878 A3 WO2014100878 A3 WO 2014100878A3 BR 2013000607 W BR2013000607 W BR 2013000607W WO 2014100878 A3 WO2014100878 A3 WO 2014100878A3
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WIPO (PCT)
Prior art keywords
genes
siliencing
cotton plants
control method
insect pests
Prior art date
Application number
PCT/BR2013/000607
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English (en)
French (fr)
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WO2014100878A8 (pt
WO2014100878A2 (pt
Inventor
Maria Fátima GROSSI DE SÁ
Alexandre Augusto PEREIRA FIRMINO
Maria Cristina Mattar Da Silva
Diogo MARTINS DE SÁ
Roberta RAMOS COELHO
Leonardo LIMA PEPINO DE MACEDO
Isabela TRISTAN LOURENÇO
Original Assignee
Embrapa - Empresa Brasileira De Pesquisa Agropecuária
Universidade Federal Do Rio Grande Do Sul - Ufrgs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Embrapa - Empresa Brasileira De Pesquisa Agropecuária, Universidade Federal Do Rio Grande Do Sul - Ufrgs filed Critical Embrapa - Empresa Brasileira De Pesquisa Agropecuária
Priority to US14/655,097 priority Critical patent/US10822609B2/en
Publication of WO2014100878A2 publication Critical patent/WO2014100878A2/pt
Publication of WO2014100878A3 publication Critical patent/WO2014100878A3/pt
Publication of WO2014100878A8 publication Critical patent/WO2014100878A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A presente invenção está relacionada ao controle de infestação de praga através da inibição ou redução da expressão de genes da família da lacase. A invenção provê ainda métodos e composições para o controle de pragas, através da alimentação de uma ou mais moléculas de RNA de fita dupla provida pela presente invenção. A invenção descreve ainda um método de obtenção de plantas transgênicas que expressem moléculas de RNA de fita dupla. A presente invenção é preferencialmente utilizada para plantas de algodoeiro.
PCT/BR2013/000607 2012-12-28 2013-12-27 Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes da família da lacase WO2014100878A2 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/655,097 US10822609B2 (en) 2012-12-28 2013-12-27 Genetic control method and compositions against insect pests in cotton plants by the silencing of genes of the laccase family

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRBR1020120335069 2012-12-28
BRBR102012033506-9A BR102012033506A2 (pt) 2012-12-28 2012-12-28 Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes da família da lacase

Publications (3)

Publication Number Publication Date
WO2014100878A2 WO2014100878A2 (pt) 2014-07-03
WO2014100878A3 true WO2014100878A3 (pt) 2014-11-13
WO2014100878A8 WO2014100878A8 (pt) 2015-01-15

Family

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PCT/BR2013/000607 WO2014100878A2 (pt) 2012-12-28 2013-12-27 Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes da família da lacase

Country Status (4)

Country Link
US (1) US10822609B2 (pt)
AR (1) AR094339A1 (pt)
BR (1) BR102012033506A2 (pt)
WO (1) WO2014100878A2 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3046716B1 (fr) 2016-01-15 2019-06-28 Universite Paris Est Creteil Val De Marne Methodes et compositions pour le controle des fourmis champignonnistes
WO2017132330A1 (en) * 2016-01-26 2017-08-03 Monsanto Technology Llc Compositions and methods for controlling insect pests
CN106884007A (zh) * 2017-03-05 2017-06-23 中国农业科学院植物保护研究所 烟粉虱med隐种漆酶lac1及其基因和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070271630A1 (en) * 2005-02-24 2007-11-22 Boukharov Andrey A Methods for genetic control of plant pest infestation and compositions thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102648283B (zh) * 2009-09-29 2018-02-16 淡马锡生命科学研究院有限公司 植物中有害动物的防治

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070271630A1 (en) * 2005-02-24 2007-11-22 Boukharov Andrey A Methods for genetic control of plant pest infestation and compositions thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BAUM JA ET AL.: "Control of coleopteran insect pest through RNA interference''.", NATURE BIOTECHNOLOGY, vol. 25, no. 11, 2007, pages 1322 - 1326 *
DATABASE GENBANK 27 June 2008 (2008-06-27), accession no. Y884062 *
HUVENNE H. ET AL.: "Mechanisms of ds RNA uptake in insects and potential of RNAi for pest control: A review.", JOURNAL OF INSECT PHYSIOLOGY., vol. 56, 2010, pages 227 - 235 *
WHYARD S ET AL.: "Ingested double-stranded RNAs can act as species- specific inseticides.", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 39, 2009, pages 824 - 832 *

Also Published As

Publication number Publication date
US20150368649A1 (en) 2015-12-24
WO2014100878A8 (pt) 2015-01-15
US10822609B2 (en) 2020-11-03
BR102012033506A2 (pt) 2014-08-26
AR094339A1 (es) 2015-07-29
WO2014100878A2 (pt) 2014-07-03

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