WO2013015717A3 - Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики - Google Patents
Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики Download PDFInfo
- Publication number
- WO2013015717A3 WO2013015717A3 PCT/RU2012/000570 RU2012000570W WO2013015717A3 WO 2013015717 A3 WO2013015717 A3 WO 2013015717A3 RU 2012000570 W RU2012000570 W RU 2012000570W WO 2013015717 A3 WO2013015717 A3 WO 2013015717A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- activity
- proteolytic enzyme
- sample
- implementation
- determining
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Neurosurgery (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EA201400156A EA028341B1 (ru) | 2011-07-26 | 2012-07-16 | Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики |
CA2842594A CA2842594C (en) | 2011-07-26 | 2012-07-16 | Method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in a heterogeneous system (variations), device for realizing same and method for diagnosing defects in the hemostatic system on the basis of a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a heterogeneous system |
JP2014522787A JP2014521952A (ja) | 2011-07-26 | 2012-07-16 | 不均一系(ばらつき)におけるタンパク質分解酵素活性の空間分布および時間分布を求めるための方法、これを実現するための装置、不均一系におけるタンパク質分解酵素活性の空間分布および時間分布の変化をもとに、止血系の欠陥を診断するための方法 |
US14/234,909 US9938563B2 (en) | 2011-07-26 | 2012-07-16 | Method for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system (variations), a device for realizing same and a method for diagnosing the defects in the hemostatic system on the basis of a change in the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system |
CN201280045249.3A CN103998620A (zh) | 2011-07-26 | 2012-07-16 | 用于确定蛋白水解酶活性的方法(变体)、用于实现该方法的装置以及诊断方法 |
EP12818440.5A EP2752495B8 (en) | 2011-07-26 | 2012-07-16 | Method for determining the activity of a proteolytic enzyme (variants), device for the implementation of same and diagnostic method |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2011131293 | 2011-07-26 | ||
RU2011131293/10A RU2518247C2 (ru) | 2011-07-26 | 2011-07-26 | Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2013015717A2 WO2013015717A2 (ru) | 2013-01-31 |
WO2013015717A3 true WO2013015717A3 (ru) | 2013-04-11 |
WO2013015717A8 WO2013015717A8 (ru) | 2014-02-20 |
Family
ID=47601696
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/RU2012/000570 WO2013015717A2 (ru) | 2011-07-26 | 2012-07-16 | Способ - определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе (варианты), устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе |
Country Status (8)
Country | Link |
---|---|
US (1) | US9938563B2 (ru) |
EP (1) | EP2752495B8 (ru) |
JP (1) | JP2014521952A (ru) |
CN (1) | CN103998620A (ru) |
CA (1) | CA2842594C (ru) |
EA (1) | EA028341B1 (ru) |
RU (1) | RU2518247C2 (ru) |
WO (1) | WO2013015717A2 (ru) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105738378A (zh) * | 2016-02-23 | 2016-07-06 | 上海航天动力科技工程有限公司 | 一种多功能自显像着色渗透剂及其应用 |
US11237178B2 (en) * | 2016-11-30 | 2022-02-01 | Obschestvo S Ogranichennoy Otvetstvennostyu <<Hemacore Labs>> | Device for monitoring the spatial and temporal dynamics of thrombin |
RU2657294C1 (ru) * | 2016-12-15 | 2018-06-13 | Государственное бюджетное учреждение здравоохранения Московской области "Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского" (ГБУЗ МО МОНИКИ им. М.Ф. Владимирского) | Устройство для количественной оценки флюоресценции и оптических свойств тканей in vivo |
JP7273631B2 (ja) * | 2019-06-26 | 2023-05-15 | 株式会社日立製作所 | 検体性状識別装置、検体性状識別方法及び検体搬送システム |
EP4270010A1 (en) * | 2020-12-28 | 2023-11-01 | Fujimori Kogyo Co., Ltd. | Method for evaluating blood coagulation performance, and method for inspecting risk of thrombosis |
CN114465969B (zh) * | 2021-12-23 | 2023-07-04 | 珠海格力电器股份有限公司 | 通讯消息组的管理方法、装置、设备和存储介质 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5059525A (en) * | 1984-11-19 | 1991-10-22 | Boehringer Mannheim Gmbh | Dry reagent for blood coagulation tests |
WO1993010261A1 (en) * | 1991-11-13 | 1993-05-27 | Dahlbaeck Bjoern | Method for the diagnosis of blood coagulation disorders |
US5339830A (en) * | 1992-01-21 | 1994-08-23 | Blake Joseph W Iii | Blood coagulation test system |
WO2009098313A1 (en) * | 2008-02-07 | 2009-08-13 | Synapse B.V. | Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1159448T3 (da) * | 1999-03-04 | 2004-08-09 | Synapse Bv | Bestemmelse af biologisk aktive former af proteolytiske enzymer |
EP2405019A1 (en) * | 2001-09-10 | 2012-01-11 | Meso Scale Technologies LLC | Methods, reagents, kits and apparatus for protein function analysis |
ES2291661T3 (es) * | 2002-05-01 | 2008-03-01 | Synapse B.V. | Prueba de diagnostico para la determinacion de la concentracion de actividad proteolitica transitoria en medios biologicos compuestos. |
EP1717588A1 (en) * | 2005-04-29 | 2006-11-02 | Synapse B.V. | Measuring thrombin activity in whole blood |
WO2007073597A1 (en) * | 2005-12-27 | 2007-07-05 | Mcmaster University | Enzyme measurement assay using a modified substrate comprising a substrate attached to a macromolecule via a spacer |
JP5461175B2 (ja) * | 2006-06-06 | 2014-04-02 | トロームビノスコープ ベスローテン フェンノートシャップ | 生体媒質中で酵素活性を決定する方法 |
WO2009055940A1 (en) * | 2007-11-02 | 2009-05-07 | Verano Financial Ltd. | Method and apparatus for monitoring spatial fibrin clot formation |
RU2395812C2 (ru) | 2008-11-14 | 2010-07-27 | Общество с ограниченной ответственностью "Медицинские инновации" | Устройство для исследования пространственного свертывания крови и ее компонентов |
-
2011
- 2011-07-26 RU RU2011131293/10A patent/RU2518247C2/ru active
-
2012
- 2012-07-16 CA CA2842594A patent/CA2842594C/en active Active
- 2012-07-16 JP JP2014522787A patent/JP2014521952A/ja active Pending
- 2012-07-16 EP EP12818440.5A patent/EP2752495B8/en active Active
- 2012-07-16 CN CN201280045249.3A patent/CN103998620A/zh active Pending
- 2012-07-16 US US14/234,909 patent/US9938563B2/en active Active
- 2012-07-16 WO PCT/RU2012/000570 patent/WO2013015717A2/ru active Application Filing
- 2012-07-16 EA EA201400156A patent/EA028341B1/ru not_active IP Right Cessation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5059525A (en) * | 1984-11-19 | 1991-10-22 | Boehringer Mannheim Gmbh | Dry reagent for blood coagulation tests |
WO1993010261A1 (en) * | 1991-11-13 | 1993-05-27 | Dahlbaeck Bjoern | Method for the diagnosis of blood coagulation disorders |
US5339830A (en) * | 1992-01-21 | 1994-08-23 | Blake Joseph W Iii | Blood coagulation test system |
WO2009098313A1 (en) * | 2008-02-07 | 2009-08-13 | Synapse B.V. | Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate |
Non-Patent Citations (2)
Title |
---|
FADEEVA O.A. ET AL.: "Thromboplastin immobilized on polystyrene surface exhibits kinetic characteristics close to those for the native protein and activates in vitro blood coagulation similarly to thromboplastin on fibroblasts.", BIOCHEMISTRY, vol. 75, no. 6, June 2010 (2010-06-01), MOSCOW, pages 734 - 743, XP055143074 * |
KONDRATOVICH A.Y. ET AL.: "Spatiotemporal dynamics of contact activation factors of blood coagulation.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1569, no. 1-3, 2002, pages 86 - 104, XP004341305 * |
Also Published As
Publication number | Publication date |
---|---|
RU2518247C2 (ru) | 2014-06-10 |
CA2842594A1 (en) | 2013-01-31 |
EA028341B1 (ru) | 2017-11-30 |
WO2013015717A2 (ru) | 2013-01-31 |
EA201400156A1 (ru) | 2014-08-29 |
EP2752495B1 (en) | 2020-03-25 |
EP2752495B8 (en) | 2020-04-29 |
CN103998620A (zh) | 2014-08-20 |
US9938563B2 (en) | 2018-04-10 |
RU2011131293A (ru) | 2013-02-10 |
WO2013015717A8 (ru) | 2014-02-20 |
JP2014521952A (ja) | 2014-08-28 |
EP2752495A4 (en) | 2015-04-15 |
US20140227726A1 (en) | 2014-08-14 |
CA2842594C (en) | 2020-01-07 |
EP2752495A2 (en) | 2014-07-09 |
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