WO2013015717A3 - Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики - Google Patents

Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики Download PDF

Info

Publication number
WO2013015717A3
WO2013015717A3 PCT/RU2012/000570 RU2012000570W WO2013015717A3 WO 2013015717 A3 WO2013015717 A3 WO 2013015717A3 RU 2012000570 W RU2012000570 W RU 2012000570W WO 2013015717 A3 WO2013015717 A3 WO 2013015717A3
Authority
WO
WIPO (PCT)
Prior art keywords
activity
proteolytic enzyme
sample
implementation
determining
Prior art date
Application number
PCT/RU2012/000570
Other languages
English (en)
French (fr)
Other versions
WO2013015717A2 (ru
WO2013015717A8 (ru
Inventor
Фазоил Иноятович АТАУЛЛАХАНОВ
Наталья Михайловна ДАШКЕВИЧ
Михаил Владимирович ОВАНЕСОВ
Василий Иванович САРБАШ
Михаил Александрович ПАНТЕЛЕЕВ
Сергей Сергеевич КАРАМЗИН
Андрей Юрьевич КОНДРАТОВИЧ
Original Assignee
Общество С Ограниченной Ответственностью "Гематологическая Корпорация"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Общество С Ограниченной Ответственностью "Гематологическая Корпорация" filed Critical Общество С Ограниченной Ответственностью "Гематологическая Корпорация"
Priority to EA201400156A priority Critical patent/EA028341B1/ru
Priority to CA2842594A priority patent/CA2842594C/en
Priority to JP2014522787A priority patent/JP2014521952A/ja
Priority to US14/234,909 priority patent/US9938563B2/en
Priority to CN201280045249.3A priority patent/CN103998620A/zh
Priority to EP12818440.5A priority patent/EP2752495B8/en
Publication of WO2013015717A2 publication Critical patent/WO2013015717A2/ru
Publication of WO2013015717A3 publication Critical patent/WO2013015717A3/ru
Publication of WO2013015717A8 publication Critical patent/WO2013015717A8/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurosurgery (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Изобретение относится к области биотехнологии. Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе in vitro, такой как кровь или плазма крови включает введение в образец люминесцентного, флуорогенного или хромогенного субстрата с последующим высвобождением детектируемой метки при расщеплении протеолитическим ферментом, и регистрирование оптических характеристик образца, что позволяет оценить пространственно-временное распределение активности фермента. Устройство для реализации заявленного способа содержит систему in vitro, средство для освещения образца, средство регистрации и средство управления. Предложен также способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в образце крови.
PCT/RU2012/000570 2011-07-26 2012-07-16 Способ - определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе (варианты), устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе WO2013015717A2 (ru)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EA201400156A EA028341B1 (ru) 2011-07-26 2012-07-16 Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики
CA2842594A CA2842594C (en) 2011-07-26 2012-07-16 Method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in a heterogeneous system (variations), device for realizing same and method for diagnosing defects in the hemostatic system on the basis of a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a heterogeneous system
JP2014522787A JP2014521952A (ja) 2011-07-26 2012-07-16 不均一系（ばらつき）におけるタンパク質分解酵素活性の空間分布および時間分布を求めるための方法、これを実現するための装置、不均一系におけるタンパク質分解酵素活性の空間分布および時間分布の変化をもとに、止血系の欠陥を診断するための方法
US14/234,909 US9938563B2 (en) 2011-07-26 2012-07-16 Method for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system (variations), a device for realizing same and a method for diagnosing the defects in the hemostatic system on the basis of a change in the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system
CN201280045249.3A CN103998620A (zh) 2011-07-26 2012-07-16 用于确定蛋白水解酶活性的方法(变体)、用于实现该方法的装置以及诊断方法
EP12818440.5A EP2752495B8 (en) 2011-07-26 2012-07-16 Method for determining the activity of a proteolytic enzyme (variants), device for the implementation of same and diagnostic method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2011131293 2011-07-26
RU2011131293/10A RU2518247C2 (ru) 2011-07-26 2011-07-26 Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе

Publications (3)

Publication Number Publication Date
WO2013015717A2 WO2013015717A2 (ru) 2013-01-31
WO2013015717A3 true WO2013015717A3 (ru) 2013-04-11
WO2013015717A8 WO2013015717A8 (ru) 2014-02-20

Family

ID=47601696

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU2012/000570 WO2013015717A2 (ru) 2011-07-26 2012-07-16 Способ - определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе (варианты), устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе

Country Status (8)

Country Link
US (1) US9938563B2 (ru)
EP (1) EP2752495B8 (ru)
JP (1) JP2014521952A (ru)
CN (1) CN103998620A (ru)
CA (1) CA2842594C (ru)
EA (1) EA028341B1 (ru)
RU (1) RU2518247C2 (ru)
WO (1) WO2013015717A2 (ru)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105738378A (zh) * 2016-02-23 2016-07-06 上海航天动力科技工程有限公司 一种多功能自显像着色渗透剂及其应用
US11237178B2 (en) * 2016-11-30 2022-02-01 Obschestvo S Ogranichennoy Otvetstvennostyu <<Hemacore Labs>> Device for monitoring the spatial and temporal dynamics of thrombin
RU2657294C1 (ru) * 2016-12-15 2018-06-13 Государственное бюджетное учреждение здравоохранения Московской области "Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского" (ГБУЗ МО МОНИКИ им. М.Ф. Владимирского) Устройство для количественной оценки флюоресценции и оптических свойств тканей in vivo
JP7273631B2 (ja) * 2019-06-26 2023-05-15 株式会社日立製作所 検体性状識別装置、検体性状識別方法及び検体搬送システム
EP4270010A1 (en) * 2020-12-28 2023-11-01 Fujimori Kogyo Co., Ltd. Method for evaluating blood coagulation performance, and method for inspecting risk of thrombosis
CN114465969B (zh) * 2021-12-23 2023-07-04 珠海格力电器股份有限公司 通讯消息组的管理方法、装置、设备和存储介质

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059525A (en) * 1984-11-19 1991-10-22 Boehringer Mannheim Gmbh Dry reagent for blood coagulation tests
WO1993010261A1 (en) * 1991-11-13 1993-05-27 Dahlbaeck Bjoern Method for the diagnosis of blood coagulation disorders
US5339830A (en) * 1992-01-21 1994-08-23 Blake Joseph W Iii Blood coagulation test system
WO2009098313A1 (en) * 2008-02-07 2009-08-13 Synapse B.V. Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1159448T3 (da) * 1999-03-04 2004-08-09 Synapse Bv Bestemmelse af biologisk aktive former af proteolytiske enzymer
EP2405019A1 (en) * 2001-09-10 2012-01-11 Meso Scale Technologies LLC Methods, reagents, kits and apparatus for protein function analysis
ES2291661T3 (es) * 2002-05-01 2008-03-01 Synapse B.V. Prueba de diagnostico para la determinacion de la concentracion de actividad proteolitica transitoria en medios biologicos compuestos.
EP1717588A1 (en) * 2005-04-29 2006-11-02 Synapse B.V. Measuring thrombin activity in whole blood
WO2007073597A1 (en) * 2005-12-27 2007-07-05 Mcmaster University Enzyme measurement assay using a modified substrate comprising a substrate attached to a macromolecule via a spacer
JP5461175B2 (ja) * 2006-06-06 2014-04-02 トロームビノスコープ ベスローテン フェンノートシャップ 生体媒質中で酵素活性を決定する方法
WO2009055940A1 (en) * 2007-11-02 2009-05-07 Verano Financial Ltd. Method and apparatus for monitoring spatial fibrin clot formation
RU2395812C2 (ru) 2008-11-14 2010-07-27 Общество с ограниченной ответственностью "Медицинские инновации" Устройство для исследования пространственного свертывания крови и ее компонентов

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059525A (en) * 1984-11-19 1991-10-22 Boehringer Mannheim Gmbh Dry reagent for blood coagulation tests
WO1993010261A1 (en) * 1991-11-13 1993-05-27 Dahlbaeck Bjoern Method for the diagnosis of blood coagulation disorders
US5339830A (en) * 1992-01-21 1994-08-23 Blake Joseph W Iii Blood coagulation test system
WO2009098313A1 (en) * 2008-02-07 2009-08-13 Synapse B.V. Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FADEEVA O.A. ET AL.: "Thromboplastin immobilized on polystyrene surface exhibits kinetic characteristics close to those for the native protein and activates in vitro blood coagulation similarly to thromboplastin on fibroblasts.", BIOCHEMISTRY, vol. 75, no. 6, June 2010 (2010-06-01), MOSCOW, pages 734 - 743, XP055143074 *
KONDRATOVICH A.Y. ET AL.: "Spatiotemporal dynamics of contact activation factors of blood coagulation.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1569, no. 1-3, 2002, pages 86 - 104, XP004341305 *

Also Published As

Publication number Publication date
RU2518247C2 (ru) 2014-06-10
CA2842594A1 (en) 2013-01-31
EA028341B1 (ru) 2017-11-30
WO2013015717A2 (ru) 2013-01-31
EA201400156A1 (ru) 2014-08-29
EP2752495B1 (en) 2020-03-25
EP2752495B8 (en) 2020-04-29
CN103998620A (zh) 2014-08-20
US9938563B2 (en) 2018-04-10
RU2011131293A (ru) 2013-02-10
WO2013015717A8 (ru) 2014-02-20
JP2014521952A (ja) 2014-08-28
EP2752495A4 (en) 2015-04-15
US20140227726A1 (en) 2014-08-14
CA2842594C (en) 2020-01-07
EP2752495A2 (en) 2014-07-09

Similar Documents

Publication Publication Date Title
WO2013015717A3 (ru) Способ определения активности протеолитического фермента (варианты), устройство для его реализации и способ диагностики
WO2013009927A3 (en) Droplet actuators and techniques for droplet-based assays
NZ726003A (en) Methods and systems for detecting an analyte or classifying a sample
WO2011120006A8 (en) Detection system for droplet-based assays
WO2012116331A3 (en) Methods and systems for haplotype determination
BR112012006757A2 (pt) detecção de ácidos nucleicos em matrizes brutas
BR112016008970A2 (pt) Método de ativação ex vivo e para avaliar ativação plasmática em um indivíduo, ensaio ex vivo para determinar atividade de calicreína plasmática em uma amostra
WO2011087760A3 (en) Processes and kits for identifying aneuploidy
WO2012076553A3 (en) Biomarkers and parameters for hypertensive disorders of pregnancy
WO2013190071A3 (en) Simultaneous measurement of thrombin generation and clot strength in plasma and whole blood
AU2011260390A8 (en) Means and methods for diagnosing pancreatic cancer in a subject
IN2014DN04645A (ru)
IN2014CN04326A (ru)
WO2011152883A3 (en) Use of bacterial beta-lactamase for in vitro diagnostics and in vivo imaging, diagnostics and therapeutics
MX2010002368A (es) Varolacion rapida de condiciones respiratorias superiores.
WO2013090386A3 (en) Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
WO2012027266A3 (en) Methods for dectecting embryonic stem cells, induced pluripotent stem cells, or cells undergoing reprogramming to produce induced pluripotent stem cells
WO2011036666A3 (en) Systems and methods for measuring translation of target proteins in cells
BR112014007556A2 (pt) método de determinação de sinais estacionários para o diagnóstico de um sistema eletromecânico
WO2011051805A8 (en) Methods and systems for identifying molecules or processes of biological interest by using knowledge discovery in biological data
BR112012023875A2 (pt) uso de acromopeptidase para lise em temperatura ambiente
WO2012166848A3 (en) Methods and compositions for the detection of functional clostridium difficile toxins
WO2012103260A3 (en) High-throughput methods to produce, validate and characterize mmabs
WO2011113813A3 (en) Assay for analytes based on aggregation
WO2012051504A3 (en) Quantitative multiplexed identification of nucleic acid targets

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12818440

Country of ref document: EP

Kind code of ref document: A2

ENP Entry into the national phase

Ref document number: 2842594

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2014522787

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 201400156

Country of ref document: EA

WWE Wipo information: entry into national phase

Ref document number: 2012818440

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 14234909

Country of ref document: US