WO2012168495A9 - Obtención de oligosacaridos mediante un procedimiento biotecnológico - Google Patents

Obtención de oligosacaridos mediante un procedimiento biotecnológico Download PDF

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Publication number
WO2012168495A9
WO2012168495A9 PCT/ES2011/070403 ES2011070403W WO2012168495A9 WO 2012168495 A9 WO2012168495 A9 WO 2012168495A9 ES 2011070403 W ES2011070403 W ES 2011070403W WO 2012168495 A9 WO2012168495 A9 WO 2012168495A9
Authority
WO
WIPO (PCT)
Prior art keywords
oligosaccharides
lacto
biotechnological process
biotechnological
obtaining
Prior art date
Application number
PCT/ES2011/070403
Other languages
English (en)
French (fr)
Other versions
WO2012168495A1 (es
Inventor
Esther MATENCIO HILLA
Verónica NAVARRO BARRERA
María ENRIQUE LÓPEZ
Marta Tortajada Serra
Dunja LUKOVIC
Diego RODRÍGUEZ JIMENEZ
Daniel RAMÓN VIDAL
Beatriz ÁLVAREZ PÉREZ
Original Assignee
Hero Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hero Ag filed Critical Hero Ag
Priority to PL11867262T priority Critical patent/PL2722394T3/pl
Priority to DK11867262.5T priority patent/DK2722394T3/en
Priority to CN201180071512.1A priority patent/CN103764835B/zh
Priority to EP11867262.5A priority patent/EP2722394B1/en
Priority to ES11867262.5T priority patent/ES2671555T3/es
Priority to PCT/ES2011/070403 priority patent/WO2012168495A1/es
Publication of WO2012168495A1 publication Critical patent/WO2012168495A1/es
Publication of WO2012168495A9 publication Critical patent/WO2012168495A9/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01146—Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (2.4.1.146)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Obtención de oligosacáridos mediante un procedimiento biotecnológico, se refiere al desarrollo de un método de producción biotecnológico de determinados oligosacáridos presentes en leche materna, concretamente la producción de los oligosacáridos lacto-N-neotetraosa y lacto-N-tetraosa a partir de lacto-N-triosa obtenida de sobrenadantes de cultivo enriquecidos en las enzimas adecuadas para generarlos a partir de sus precursores: lactosa y N-acetil-glucosamina.
PCT/ES2011/070403 2011-06-07 2011-06-07 Obtención de oligosacaridos mediante un procedimiento biotecnológico WO2012168495A1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
PL11867262T PL2722394T3 (pl) 2011-06-07 2011-06-07 Otrzymywanie oligosacharydów za pomocą procesu biotechnologicznego
DK11867262.5T DK2722394T3 (en) 2011-06-07 2011-06-07 OBJECTIVES OF OLIGOSACCHARIDES BY A BIOTECHNOLOGICAL PROCEDURE
CN201180071512.1A CN103764835B (zh) 2011-06-07 2011-06-07 通过生物技术方法获取低聚糖
EP11867262.5A EP2722394B1 (en) 2011-06-07 2011-06-07 Obtaining oligosaccharides by means of a biotechnological process
ES11867262.5T ES2671555T3 (es) 2011-06-07 2011-06-07 Obtención de oligosacáridos mediante un proceso biotecnológico
PCT/ES2011/070403 WO2012168495A1 (es) 2011-06-07 2011-06-07 Obtención de oligosacaridos mediante un procedimiento biotecnológico

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/ES2011/070403 WO2012168495A1 (es) 2011-06-07 2011-06-07 Obtención de oligosacaridos mediante un procedimiento biotecnológico

Publications (2)

Publication Number Publication Date
WO2012168495A1 WO2012168495A1 (es) 2012-12-13
WO2012168495A9 true WO2012168495A9 (es) 2013-09-06

Family

ID=47295521

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2011/070403 WO2012168495A1 (es) 2011-06-07 2011-06-07 Obtención de oligosacaridos mediante un procedimiento biotecnológico

Country Status (6)

Country Link
EP (1) EP2722394B1 (es)
CN (1) CN103764835B (es)
DK (1) DK2722394T3 (es)
ES (1) ES2671555T3 (es)
PL (1) PL2722394T3 (es)
WO (1) WO2012168495A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106536726A (zh) * 2014-07-14 2017-03-22 巴斯夫欧洲公司 LNT、LNnT和其岩藻糖基化衍生物的生物技术生产

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015032413A1 (en) * 2013-09-06 2015-03-12 Glycom A/S Fermentative production of oligosaccharides
PL2896628T3 (pl) 2014-01-20 2019-03-29 Jennewein Biotechnologie Gmbh Sposób wydajnego oczyszczania obojętnych oligosacharydów ludzkiego mleka (HMO) z fermentacji mikrobiologicznej
EP3141610A1 (en) 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
US11312741B2 (en) 2016-04-19 2022-04-26 Glycom A/S Separation of oligosaccharides from fermentation broth
WO2017221208A1 (en) * 2016-06-24 2017-12-28 Glycom A/S Compositions comprising hmos, their production and use for the prevention and/or treatment of viral and/or bacterial infections
US12065462B2 (en) 2017-06-30 2024-08-20 Glycom A/S Purification of oligosaccharides
EP3652189A4 (en) 2017-07-12 2021-04-21 Glycom A/S AMORPHIC MIXTURE CONSISTING OF A NEUTRAL MONO- OR OLIGOSACCHARIDE AND A NON-GLUCIDIC ACID COMPONENT
EP3670662A1 (en) * 2018-12-20 2020-06-24 Basf Se Enzymatic hexosaminidation of lactose
CN116261597A (zh) 2020-08-10 2023-06-13 因比奥斯公司 α-1,3糖基化形式的Fuc-a1,2-Gal-R的产生
ES2966260T3 (es) * 2020-08-10 2024-04-19 Inbiose Nv Producción de una mezcla de oligosacáridos neutros no fucosilados por una célula
CN111979168B (zh) * 2020-08-17 2022-07-22 江南大学 一种提高乳酰-n-三糖ii产量的基因工程菌及生产方法
CN116042562B (zh) * 2022-03-11 2023-10-20 山东恒鲁生物科技有限公司 重组酵母菌及其应用
CN117866789A (zh) * 2023-01-05 2024-04-12 山东恒鲁生物科技有限公司 一种合成二糖的酵母基因工程菌
CN117867054B (zh) * 2023-01-09 2024-09-27 山东恒鲁生物科技有限公司 合成hmo的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5874261A (en) 1988-09-02 1999-02-23 The Trustees Of The University Of Pennsylvania Method for the purification of glycosyltransferases
WO1995002683A1 (en) 1993-07-15 1995-01-26 Neose Pharmaceuticals Method of synthesizing saccharide compositions
US6204431B1 (en) 1994-03-09 2001-03-20 Abbott Laboratories Transgenic non-human mammals expressing heterologous glycosyltransferase DNA sequences produce oligosaccharides and glycoproteins in their milk
JPH11253191A (ja) 1998-03-13 1999-09-21 Meiji Milk Prod Co Ltd ラクト−ｎ−テトラオースの合成法
FR2796082B1 (fr) 1999-07-07 2003-06-27 Centre Nat Rech Scient Procede de production d'oligosaccharides
CA2548140A1 (en) * 2003-12-05 2005-06-23 University Of Massachusetts Oligosaccharide compositions and use thereof in the treatment of infection
WO2008120341A1 (ja) * 2007-03-29 2008-10-09 Advantest Corporation 電子銃及び電子ビーム露光装置

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106536726A (zh) * 2014-07-14 2017-03-22 巴斯夫欧洲公司 LNT、LNnT和其岩藻糖基化衍生物的生物技术生产

Also Published As

Publication number Publication date
WO2012168495A1 (es) 2012-12-13
CN103764835A (zh) 2014-04-30
ES2671555T3 (es) 2018-06-07
EP2722394A1 (en) 2014-04-23
EP2722394A4 (en) 2015-03-25
DK2722394T3 (en) 2018-06-18
PL2722394T3 (pl) 2018-09-28
EP2722394B1 (en) 2018-03-21
CN103764835B (zh) 2016-08-17

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