SG10201808050YA - BIOTECHNOLOGICAL PRODUCTION OF LNT, LNnT AND THE FUCOSYLATED DERIVATIVES THEREOF - Google Patents
BIOTECHNOLOGICAL PRODUCTION OF LNT, LNnT AND THE FUCOSYLATED DERIVATIVES THEREOFInfo
- Publication number
- SG10201808050YA SG10201808050YA SG10201808050YA SG10201808050YA SG10201808050YA SG 10201808050Y A SG10201808050Y A SG 10201808050YA SG 10201808050Y A SG10201808050Y A SG 10201808050YA SG 10201808050Y A SG10201808050Y A SG 10201808050YA SG 10201808050Y A SG10201808050Y A SG 10201808050YA
- Authority
- SG
- Singapore
- Prior art keywords
- lnnt
- lnt
- biotechnological production
- fucosylated derivatives
- lacto
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01022—Lactose synthase (2.4.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01062—Ganglioside galactosyltransferase (2.4.1.62)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01146—Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (2.4.1.146)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01052—Fucokinase (2.7.1.52)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/0703—Fucose-1-phosphate guanylyltransferase (2.7.7.30)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07064—UTP-monosaccharide-1-phosphate uridylyltransferase (2.7.7.64)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y501/00—Racemaces and epimerases (5.1)
- C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
- C12Y501/03002—UDP-glucose 4-epimerase (5.1.3.2), i.e. UDP-galactose 4-epimerase
Abstract
Biotechnological production of LNT, LNnT and the fucosylated derivatives thereof The invention relates to primarily genetically engineered microorganisms for the in-vivo synthesis of lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT) and the fucosylated derivatives thereof, and to uses of said microorganisms in methods for producing lacto-N-tetraose and lacto-N-neotetraose and the fucosylated derivatives thereof. [No suitable figure]
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14176958 | 2014-07-14 | ||
EP14198960 | 2014-12-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201808050YA true SG10201808050YA (en) | 2018-10-30 |
Family
ID=52823645
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201808050YA SG10201808050YA (en) | 2014-07-14 | 2015-04-10 | BIOTECHNOLOGICAL PRODUCTION OF LNT, LNnT AND THE FUCOSYLATED DERIVATIVES THEREOF |
SG11201700296YA SG11201700296YA (en) | 2014-07-14 | 2015-04-10 | Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201700296YA SG11201700296YA (en) | 2014-07-14 | 2015-04-10 | Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof |
Country Status (14)
Country | Link |
---|---|
US (1) | US20170204443A1 (en) |
EP (1) | EP3169775A1 (en) |
JP (1) | JP2017520267A (en) |
KR (1) | KR20170028438A (en) |
CN (1) | CN106536726A (en) |
AU (1) | AU2015291487A1 (en) |
BR (1) | BR112017000596A2 (en) |
CA (1) | CA2954456A1 (en) |
IL (1) | IL249972A0 (en) |
MX (1) | MX2017000621A (en) |
RU (1) | RU2017104525A (en) |
SG (2) | SG10201808050YA (en) |
WO (1) | WO2016008602A1 (en) |
ZA (1) | ZA201700935B (en) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114052237A (en) * | 2014-08-19 | 2022-02-18 | 谱赛科有限责任公司 | Method for preparing rebaudioside I and application |
DE202017007248U1 (en) | 2016-04-19 | 2020-04-23 | Glycom A/S | Separation of oligosaccharides from the fermentation broth |
WO2017221208A1 (en) | 2016-06-24 | 2017-12-28 | Glycom A/S | Compositions comprising hmos, their production and use for the prevention and/or treatment of viral and/or bacterial infections |
US10407516B2 (en) | 2016-10-31 | 2019-09-10 | Glycom A/S | Crystalline HMO |
WO2019003135A1 (en) | 2017-06-30 | 2019-01-03 | Glycom A/S | Purification of oligosaccharides |
EP3425052A1 (en) | 2017-07-07 | 2019-01-09 | Jennewein Biotechnologie GmbH | Fucosyltransferases and their use in producing fucosylated oligosaccharides |
EP3652189A4 (en) | 2017-07-12 | 2021-04-21 | Glycom A/S | Amorphous mixture comprising a neutral mono- or oligosaccharide and an acidic non-carbohydrate component |
CN108410787A (en) * | 2018-03-13 | 2018-08-17 | 光明乳业股份有限公司 | A kind of recombined bacillus subtilis of synthesis new tetroses of lactoyl-N- and its construction method and application |
EP3891291A4 (en) * | 2018-12-04 | 2022-10-26 | Glycom A/S | Synthesis of the fucosylated oligosaccharide lnfp-v |
CN109735479B (en) * | 2019-01-30 | 2022-04-01 | 光明乳业股份有限公司 | Recombinant bacillus subtilis for synthesizing 2' -fucosyllactose and construction method and application thereof |
CN109735480B (en) * | 2019-02-27 | 2021-11-12 | 光明乳业股份有限公司 | Recombinant bacillus subtilis for synthesizing lactyl-N-neotetraose and construction method and application thereof |
CN109825465B (en) * | 2019-02-27 | 2021-11-12 | 光明乳业股份有限公司 | Recombinant bacillus subtilis for synthesizing lactyl-N-neotetraose based on balanced UDP-sugar supply and construction method and application thereof |
CN115485387A (en) | 2019-12-19 | 2022-12-16 | 巴斯夫欧洲公司 | Increasing the space-time yield, the carbon conversion efficiency and the carbon substrate flexibility in the production of fine chemicals |
WO2021199445A1 (en) * | 2020-04-03 | 2021-10-07 | Rensselaer Polytechnic Institute | Method for producing sulfated polysaccharide and method for producing paps |
CN111979168B (en) * | 2020-08-17 | 2022-07-22 | 江南大学 | Genetic engineering bacterium for improving yield of lactoyl-N-trisaccharide II and production method |
WO2023141513A2 (en) * | 2022-01-19 | 2023-07-27 | The Regents Of The University Of California | Functionalized human milk oligosaccharides and methods for producing them |
WO2023168441A1 (en) * | 2022-03-04 | 2023-09-07 | The Regents Of The University Of California | Production of milk oligosaccharides in plants |
WO2023219437A1 (en) * | 2022-05-11 | 2023-11-16 | (주)에이피테크놀로지 | Method for producing lacto-n-tetraose and lacto-n-neotetraose using corynebacterium glutamicum |
KR102645729B1 (en) * | 2022-05-11 | 2024-03-12 | (주)에이피테크놀로지 | Method for producing Lacto-N-tetraose and Lacto-N-neotetraose using Corynebacterium glutamicum |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3403205B2 (en) * | 1996-09-17 | 2003-05-06 | 協和醗酵工業株式会社 | Process for producing sugar nucleotides and glycoconjugates |
JPH11221079A (en) * | 1998-02-04 | 1999-08-17 | Kyowa Hakko Kogyo Co Ltd | Transglycosidase and dna encoding the same enzyme |
JP4551041B2 (en) * | 1999-06-29 | 2010-09-22 | 協和発酵キリン株式会社 | Useful polypeptides |
FR2796082B1 (en) | 1999-07-07 | 2003-06-27 | Centre Nat Rech Scient | PROCESS FOR PRODUCING OLIGOSACCHARIDES |
WO2002018602A1 (en) * | 2000-09-01 | 2002-03-07 | Kyowa Hakko Kogyo Co., Ltd. | Novel polypeptide |
JP3890556B2 (en) * | 2001-11-28 | 2007-03-07 | 富士フイルム株式会社 | Nucleic acid separation and purification method and nucleic acid separation and purification unit |
BRPI0923433B1 (en) * | 2008-12-19 | 2021-06-01 | Jennewein Biotechnologie Gmbh | METHOD TO PRODUCE A GENETICALLY MODIFIED CELL HAVING THE ABILITY TO PRODUCE FUCOSYLATE COMPOUNDS AND METHOD TO MAKE A FUCOSYLATE COMPOUND |
EP2454948A1 (en) * | 2010-11-23 | 2012-05-23 | Nestec S.A. | Oligosaccharide mixture and food product comprising this mixture, especially infant formula |
EP2675899A4 (en) * | 2011-02-16 | 2014-09-10 | Glycosyn LLC | Biosynthesis of human milk oligosaccharides in engineered bacteria |
PL2722394T3 (en) * | 2011-06-07 | 2018-09-28 | Hero Ag | Obtaining oligosaccharides by means of a biotechnological process |
WO2013182206A1 (en) | 2012-06-08 | 2013-12-12 | Glycom A/S | Method for producing oligosaccharides and oligosaccharide glycosides by fermentation |
EP4166650A1 (en) * | 2013-03-14 | 2023-04-19 | Glycosyn LLC | Methods for producing n-acetylglucosamine-containing oligosaccharides |
-
2015
- 2015-04-10 EP EP15715264.6A patent/EP3169775A1/en active Pending
- 2015-04-10 SG SG10201808050YA patent/SG10201808050YA/en unknown
- 2015-04-10 KR KR1020177003988A patent/KR20170028438A/en not_active Application Discontinuation
- 2015-04-10 MX MX2017000621A patent/MX2017000621A/en unknown
- 2015-04-10 CN CN201580038218.9A patent/CN106536726A/en active Pending
- 2015-04-10 BR BR112017000596-4A patent/BR112017000596A2/en not_active Application Discontinuation
- 2015-04-10 AU AU2015291487A patent/AU2015291487A1/en not_active Abandoned
- 2015-04-10 RU RU2017104525A patent/RU2017104525A/en unknown
- 2015-04-10 CA CA2954456A patent/CA2954456A1/en not_active Abandoned
- 2015-04-10 US US15/324,309 patent/US20170204443A1/en not_active Abandoned
- 2015-04-10 SG SG11201700296YA patent/SG11201700296YA/en unknown
- 2015-04-10 WO PCT/EP2015/057805 patent/WO2016008602A1/en active Application Filing
- 2015-04-10 JP JP2017501685A patent/JP2017520267A/en not_active Withdrawn
-
2017
- 2017-01-08 IL IL249972A patent/IL249972A0/en unknown
- 2017-02-07 ZA ZA2017/00935A patent/ZA201700935B/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2016008602A1 (en) | 2016-01-21 |
EP3169775A1 (en) | 2017-05-24 |
JP2017520267A (en) | 2017-07-27 |
BR112017000596A2 (en) | 2018-01-23 |
CN106536726A (en) | 2017-03-22 |
AU2015291487A1 (en) | 2017-02-16 |
KR20170028438A (en) | 2017-03-13 |
US20170204443A1 (en) | 2017-07-20 |
ZA201700935B (en) | 2018-08-29 |
IL249972A0 (en) | 2017-03-30 |
RU2017104525A3 (en) | 2018-12-12 |
MX2017000621A (en) | 2017-04-27 |
CA2954456A1 (en) | 2016-01-21 |
RU2017104525A (en) | 2018-08-14 |
SG11201700296YA (en) | 2017-02-27 |
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