WO2012144826A3 - 생체분자 상호작용 검출 시스템 및 이를 이용한 검출 방법 - Google Patents

생체분자 상호작용 검출 시스템 및 이를 이용한 검출 방법 Download PDF

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Publication number
WO2012144826A3
WO2012144826A3 PCT/KR2012/003010 KR2012003010W WO2012144826A3 WO 2012144826 A3 WO2012144826 A3 WO 2012144826A3 KR 2012003010 W KR2012003010 W KR 2012003010W WO 2012144826 A3 WO2012144826 A3 WO 2012144826A3
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WO
WIPO (PCT)
Prior art keywords
droplet
microfluidic system
based microfluidic
oil
same
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PCT/KR2012/003010
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English (en)
French (fr)
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WO2012144826A2 (ko
Inventor
장수익
최재원
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충북대학교 산학협력단
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Publication of WO2012144826A2 publication Critical patent/WO2012144826A2/ko
Publication of WO2012144826A3 publication Critical patent/WO2012144826A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

액적기반 미세유체시스템 및 그 형광 편광 측정방법이 개시된다. 본 발명에 따른 액적기반 미세유체시스템은, 형광물질이 표지된 제1 생체분자 및 형광물질이 표지되지 않은 제2 생체분자를 흘려 주입하는 시료 주입구, 상기 시료와 섞이지 않는 다른 상 물질인 오일을 흘려 주입하는 오일 주입구, 및 상기 시료 및 오일에 기초하여 액적을 형성하는 액적 형성부를 포함하는 액적기반 미세유체시스템; 및 상기 액적기반 미세유체시스템에 의해 형성된 액적으로부터 방사되는 형광 편광도를 측정하는 형광 편광 측정부를 포함하는 것을 특징으로 한다.
PCT/KR2012/003010 2011-04-21 2012-04-19 생체분자 상호작용 검출 시스템 및 이를 이용한 검출 방법 WO2012144826A2 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20110037033 2011-04-21
KR10-2011-0037033 2011-04-21
KR10-2012-0039194 2012-04-16
KR1020120039194A KR101356279B1 (ko) 2011-04-21 2012-04-16 생체분자 상호작용 검출 시스템 및 이를 이용한 검출 방법

Publications (2)

Publication Number Publication Date
WO2012144826A2 WO2012144826A2 (ko) 2012-10-26
WO2012144826A3 true WO2012144826A3 (ko) 2013-01-17

Family

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Application Number Title Priority Date Filing Date
PCT/KR2012/003010 WO2012144826A2 (ko) 2011-04-21 2012-04-19 생체분자 상호작용 검출 시스템 및 이를 이용한 검출 방법

Country Status (2)

Country Link
KR (1) KR101356279B1 (ko)
WO (1) WO2012144826A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106770123B (zh) * 2017-01-04 2021-06-18 新疆维吾尔自治区产品质量监督检验研究院 流动注射荧光法鉴别地沟油的装置及其方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160138699A (ko) 2015-05-26 2016-12-06 충북대학교 산학협력단 액적 기반의 형광 편광 면역분석을 이용한 단백질의 검출 및 정량 방법
KR102039793B1 (ko) * 2017-10-20 2019-11-01 숙명여자대학교산학협력단 생체분자의 결합여부 예측방법
KR102278427B1 (ko) * 2019-11-21 2021-07-16 주식회사 바이오티엔에스 형광 검출 장치 및 이를 이용한 형광 검출 방법
CN114778589B (zh) * 2022-06-23 2022-09-30 中国科学技术大学 一种纵向弛豫速率测量系统及利用该系统的测量方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4916080A (en) * 1985-11-08 1990-04-10 Hitachi, Ltd. Immunoassay with antigen or antibody labelled microcapsules containing fluorescent substance
KR20060058638A (ko) * 2004-11-25 2006-05-30 한국과학기술연구원 랩온어칩에서의 형광 편광 측정 방법 및 장치
US20080003142A1 (en) * 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
WO2009026448A1 (en) * 2007-08-21 2009-02-26 Affomix Corporation Interaction screening methods, systems and devices
US20110000560A1 (en) * 2009-03-23 2011-01-06 Raindance Technologies, Inc. Manipulation of Microfluidic Droplets

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4916080A (en) * 1985-11-08 1990-04-10 Hitachi, Ltd. Immunoassay with antigen or antibody labelled microcapsules containing fluorescent substance
KR20060058638A (ko) * 2004-11-25 2006-05-30 한국과학기술연구원 랩온어칩에서의 형광 편광 측정 방법 및 장치
US20080003142A1 (en) * 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
WO2009026448A1 (en) * 2007-08-21 2009-02-26 Affomix Corporation Interaction screening methods, systems and devices
US20110000560A1 (en) * 2009-03-23 2011-01-06 Raindance Technologies, Inc. Manipulation of Microfluidic Droplets

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106770123B (zh) * 2017-01-04 2021-06-18 新疆维吾尔自治区产品质量监督检验研究院 流动注射荧光法鉴别地沟油的装置及其方法

Also Published As

Publication number Publication date
WO2012144826A2 (ko) 2012-10-26
KR101356279B1 (ko) 2014-01-28
KR20120120023A (ko) 2012-11-01

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