WO2012120176A3 - Proteínas recombinantes con efecto antitumoral - Google Patents

Proteínas recombinantes con efecto antitumoral Download PDF

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Publication number
WO2012120176A3
WO2012120176A3 PCT/ES2012/070150 ES2012070150W WO2012120176A3 WO 2012120176 A3 WO2012120176 A3 WO 2012120176A3 ES 2012070150 W ES2012070150 W ES 2012070150W WO 2012120176 A3 WO2012120176 A3 WO 2012120176A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant proteins
antitumor effects
vector
sequence
relates
Prior art date
Application number
PCT/ES2012/070150
Other languages
English (en)
French (fr)
Other versions
WO2012120176A2 (es
Inventor
María VILANOVA BRUGUES
Antoni BENITO MUNDET
Marc RIBÓ PANOSA
Jessica CASTRO GALLEGOS
Pere TUBERT JUHE
Anna VERT COMPANY
Original Assignee
Universitat De Girona
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat De Girona filed Critical Universitat De Girona
Publication of WO2012120176A2 publication Critical patent/WO2012120176A2/es
Publication of WO2012120176A3 publication Critical patent/WO2012120176A3/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/27—Endoribonucleases producing 3'-phosphomonoesters (3.1.27)
    • C12Y301/27005—Pancreatic ribonuclease (3.1.27.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención proporciona una proteína recombinante con actividad ribonucleasa que comprende una secuencia derivada de la ribonucleasa pancreática humana (RNasa-PH) de secuencia SEC ID NO: 37 fusionada a través de su extremo N-terminal a una secuencia de localización nuclear convencional, así como una molécula de ADN recombinante que codifica para esta proteína, un vector que comprende dicha molécula de ADN y una célula que comprende dicho vector. Adicionalmente la presente invención está referida a un procedimiento para la obtención de la proteína recombinante y el uso de la misma como agente antitumoral.
PCT/ES2012/070150 2011-03-10 2012-03-08 Proteínas recombinantes con efecto antitumoral WO2012120176A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP201130324 2011-03-10
ES201130324A ES2388020B1 (es) 2011-03-10 2011-03-10 Proteínas recombinantes con efecto antitumoral.

Publications (2)

Publication Number Publication Date
WO2012120176A2 WO2012120176A2 (es) 2012-09-13
WO2012120176A3 true WO2012120176A3 (es) 2012-11-01

Family

ID=46798596

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2012/070150 WO2012120176A2 (es) 2011-03-10 2012-03-08 Proteínas recombinantes con efecto antitumoral

Country Status (2)

Country Link
ES (1) ES2388020B1 (es)
WO (1) WO2012120176A2 (es)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004284977A (ja) * 2003-03-20 2004-10-14 Masakazu Ueda ヒト生理活性物質とヒト蛋白質合成阻害物質との挿入融合体
US20050261232A1 (en) * 2004-04-13 2005-11-24 Quintessence Biosciences, Inc. Non-natural ribonuclease conjugates as cytotoxic agents
WO2006138558A2 (en) * 2005-06-16 2006-12-28 Wisconsin Alumni Research Foundation Cytotoxic ribonuclease variants
JP2010180174A (ja) * 2009-02-06 2010-08-19 Tokyo Univ Of Science 膵癌の診断方法および膵癌の診断用キット
WO2011161260A1 (en) * 2010-06-25 2011-12-29 Adamed Sp. Z O.O. Anticancer fusion protein

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004284977A (ja) * 2003-03-20 2004-10-14 Masakazu Ueda ヒト生理活性物質とヒト蛋白質合成阻害物質との挿入融合体
US20050261232A1 (en) * 2004-04-13 2005-11-24 Quintessence Biosciences, Inc. Non-natural ribonuclease conjugates as cytotoxic agents
WO2006138558A2 (en) * 2005-06-16 2006-12-28 Wisconsin Alumni Research Foundation Cytotoxic ribonuclease variants
JP2010180174A (ja) * 2009-02-06 2010-08-19 Tokyo Univ Of Science 膵癌の診断方法および膵癌の診断用キット
WO2011161260A1 (en) * 2010-06-25 2011-12-29 Adamed Sp. Z O.O. Anticancer fusion protein

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE 0 "Human pancreas ribonucleasel (RNasel) sequence, SEQ ID I", accession no. YG98365 *
DATABASE 0 "Human pancreatic ribonucleasel (RNase 1) chain X , SEQ ID 2", accession no. ZS55938 *
DATABASE 0 "Human RNase 1 protein SEQID N° 4", accession no. DS52279 *
DATABASE ACCESSIONNUMBER accession no. EE02927 *
PERE TUBERT ET AL.: "The nuclear transport capacity of a human-pancreatic ribonuclease variant is critical for its cytotoxicity. Investigational New Drugs", THE JOURNAL OF NEW ANTICANCER AGENTS., vol. 29, no. 5, October 2011 (2011-10-01), pages 811 - 817 *

Also Published As

Publication number Publication date
ES2388020B1 (es) 2013-10-01
ES2388020A1 (es) 2012-10-05
WO2012120176A2 (es) 2012-09-13

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