WO2012098772A1 - 殺菌方法、殺菌装置および光を利用する殺菌剤 - Google Patents
殺菌方法、殺菌装置および光を利用する殺菌剤 Download PDFInfo
- Publication number
- WO2012098772A1 WO2012098772A1 PCT/JP2011/077865 JP2011077865W WO2012098772A1 WO 2012098772 A1 WO2012098772 A1 WO 2012098772A1 JP 2011077865 W JP2011077865 W JP 2011077865W WO 2012098772 A1 WO2012098772 A1 WO 2012098772A1
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- WIPO (PCT)
- Prior art keywords
- light
- proanthocyanidin
- sterilizing
- bactericidal
- proanthocyanidins
- Prior art date
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 42
- 239000000645 desinfectant Substances 0.000 title claims abstract description 10
- JPFCOVZKLAXXOE-XBNSMERZSA-N (3r)-2-(3,5-dihydroxy-4-methoxyphenyl)-8-[(2r,3r,4r)-3,5,7-trihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2h-chromen-4-yl]-3,4-dihydro-2h-chromene-3,5,7-triol Chemical compound C1=C(O)C(OC)=C(O)C=C1C1[C@H](O)CC(C(O)=CC(O)=C2[C@H]3C4=C(O)C=C(O)C=C4O[C@@H]([C@@H]3O)C=3C=CC(O)=CC=3)=C2O1 JPFCOVZKLAXXOE-XBNSMERZSA-N 0.000 claims abstract description 35
- 229920001991 Proanthocyanidin Polymers 0.000 claims abstract description 35
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- 235000005487 catechin Nutrition 0.000 claims abstract description 33
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical group OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims description 57
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- 230000000694 effects Effects 0.000 abstract description 11
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- 238000006243 chemical reaction Methods 0.000 description 10
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- 238000004435 EPR spectroscopy Methods 0.000 description 9
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- 238000012360 testing method Methods 0.000 description 6
- XMOCLSLCDHWDHP-IUODEOHRSA-N epi-Gallocatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-IUODEOHRSA-N 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
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- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 4
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- DZYNKLUGCOSVKS-UHFFFAOYSA-N epigallocatechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3cc(O)c(O)c(O)c3 DZYNKLUGCOSVKS-UHFFFAOYSA-N 0.000 description 3
- LVJJFMLUMNSUFN-UHFFFAOYSA-N gallocatechin gallate Natural products C1=C(O)C=C2OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C1OC(=O)C1=CC(O)=C(O)C(O)=C1 LVJJFMLUMNSUFN-UHFFFAOYSA-N 0.000 description 3
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- 230000002292 Radical scavenging effect Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
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- 230000000249 desinfective effect Effects 0.000 description 2
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- 231100001231 less toxic Toxicity 0.000 description 2
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- 230000002829 reductive effect Effects 0.000 description 2
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- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- WMBWREPUVVBILR-GHTZIAJQSA-N (+)-gallocatechin gallate Chemical compound O([C@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-GHTZIAJQSA-N 0.000 description 1
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
- A61L2/186—Peroxide solutions
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/084—Visible light
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/085—Infrared radiation
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- A—HUMAN NECESSITIES
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- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
Definitions
- the present invention relates to a sterilization method, a sterilization apparatus, and a bactericide using light.
- catechins have a bactericidal action. This is because catechins generate hydrogen peroxide by reducing dissolved oxygen, and this hydrogen peroxide exerts a bactericidal action (see, for example, Non-Patent Document 1).
- catechins epigallocatechin, shrimp catechin gallate, and epigallocatechin gallate having a gallate group have been confirmed to have high affinity for cell membranes and strong bactericidal action (for example, Non-Patent Document 2). Or see 3).
- Non-Patent Documents 1 to 3 catechins have a bactericidal action and exert a bactericidal effect even at low concentrations.
- a long action time of 12 hours or more is necessary.
- the present invention has been made paying attention to such a problem, and an object thereof is to provide a sterilization method, a sterilization apparatus, and a bactericide using light that can obtain a high sterilization effect in a short time.
- the sterilization method according to the present invention is characterized in that a sterilizing agent containing catechins is brought into contact with an object to be sterilized and then irradiated with light.
- the sterilization method according to the present invention can obtain a sterilization effect according to the following principle. That is, as shown in FIG. 1, catechins generate hydrogen peroxide (H 2 O 2 ) by reducing dissolved oxygen (O 2 ) and exhibit antibacterial activity. When this hydrogen peroxide is irradiated with light, as shown in FIG. 2, the hydrogen peroxide is photodegraded to generate hydroxyl radicals (.OH). At this time, since catechins have a high antioxidant action, their phenolic hydroxyl groups act as donors for e ⁇ and H + , while also acting as hydroxyl radical scavenging sites, and have a bactericidal effect. Usually not considered. However, as shown in FIG.
- the phenolic hydroxyl group after acting as a donor finally becomes a quinone structure, and thus the hydroxyl radical cannot be eliminated.
- the phenolic hydroxyl group acts as a donor and does not eliminate the hydroxyl radical, so that a bactericidal effect by the hydroxyl radical is obtained.
- the present inventors have found a bactericidal effect in a method that cannot normally be expected to obtain a bactericidal effect, and have reached the present invention.
- hydroxyl radicals can be generated by irradiating the sterilizer with light. For this reason, the bactericidal effect by a hydroxyl radical is acquired and the sterilization object can be sterilized. In addition, a higher sterilization effect can be obtained in a shorter time than the sterilization action by catechins when light is not irradiated by the generated hydroxyl radical.
- the method of bringing the sterilizing agent into contact with the sterilizing target is any method such as a method of applying or spraying the sterilizing agent, or a method of immersing the sterilizing target in a solution of the sterilizing agent. Also good.
- the catechins may be composed of one of catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallocatechin, catechin gallate and gallocatechin gallate. Even if it consists of the above combination, it may consist of what superposed
- catechins preferably have a gallate group. Since catechins having a gallate group have high affinity for cell membranes, they have a high bactericidal effect on sterilized objects having cell membranes. In addition, since the amount of hydrogen peroxide generated by reducing dissolved oxygen is larger than when there is no gallate group, the amount of hydroxyl radicals generated by light irradiation also increases, and the bactericidal effect can be further enhanced. .
- the sterilizing agent may be composed of only catechins or may contain other substances.
- Other substances may be water, fungicides, sugars, colorants, flavors, seasonings, synthetic or natural fungicides other than catechins, or any other substance.
- bactericides other than catechins strong acid water, iodine agent (for example, iodine tincture, povidone iodine, etc.), chlorine (for example, sodium hypochlorite, etc.), mercurochrome solution, chlorhexidine gluconate, acrinol, alcohols (for example, And ethyl alcohol) and hydrogen peroxide.
- iodine agent for example, iodine tincture, povidone iodine, etc.
- chlorine for example, sodium hypochlorite, etc.
- mercurochrome solution chlorhexidine gluconate
- acrinol for example, And ethyl alcohol
- hydrogen peroxide it is more
- the bactericidal agent containing catechins is preferably composed of a solution containing catechins, particularly preferably an aqueous proanthocyanidin solution. Further, the proanthocyanidin aqueous solution preferably has a proanthocyanidin concentration of 0.25 to 4 mg / mL. Since proanthocyanidins are substances obtained by polymerizing a plurality of catechins, they can obtain a particularly high bactericidal effect and have high safety.
- the light may be light of any wavelength, such as ultraviolet light or infrared light, as long as hydroxyl radicals can be generated from hydrogen peroxide, but the wavelength is particularly 350 nm to 500 nm. Preferably there is. Also in this case, a high bactericidal effect can be obtained and safety is high. In particular, safety can be further enhanced by using visible light.
- the irradiance of the irradiated light is preferably 300 mW / cm 2 or more, and the larger the value, the more effective.
- the sterilizer according to the present invention irradiates light having a wavelength of 350 nm to 500 nm to a sterilizer composed of a proanthocyanidin aqueous solution having a proanthocyanidin concentration of 0.25 to 4 mg / mL and the sterilizer brought into contact with the sterilization target. And a light emitting means provided in a possible manner.
- the sterilization apparatus according to the present invention can suitably carry out the sterilization method according to the present invention.
- hydroxyl radicals can be generated by bringing a sterilizing agent into contact with an object to be sterilized and then irradiating the sterilizing agent with light by a light emitting means. For this reason, the high sterilization effect by a hydroxyl radical is acquired, and the sterilization object can be sterilized.
- Proanthocyanidins are also highly safe because of their low toxicity.
- the light emitting means for irradiating light is, for example, a heating lamp, a fluorescent lamp, a halogen lamp, a xenon lamp, an LED (light emitting diode), a semiconductor laser, a device using sunlight, It can be anything. Further, the light to be irradiated may be light having a single wavelength, light including a plurality of wavelengths, or light having a wavelength in a predetermined band.
- the light sterilizing agent using the light according to the present invention is characterized by containing a proanthocyanidin aqueous solution, and particularly characterized by having a proanthocyanidin concentration of 0.25 to 4 mg / mL.
- the bactericidal agent using light according to the present invention is suitably used as a bactericidal agent for the sterilizing method and sterilizing apparatus according to the present invention.
- the disinfectant using light according to the present invention is used by being applied to or sprayed on the object to be sterilized and brought into contact with the object to be sterilized. Then, by irradiating light, a hydroxyl radical can be produced
- Proanthocyanidins are also highly safe because of their low toxicity.
- the sterilizing method and sterilizing device according to the present invention and the sterilizing agent using light according to the present invention are preferably selected as appropriate according to the sterilization target.
- the sterilizing agent is preferably composed of toothpaste, mouthwash, rinse agent for dental treatment, and the like.
- the present invention it is possible to provide a sterilizing method, a sterilizing apparatus, and a bactericide using light that can obtain a high sterilizing effect in a short time.
- the disinfectant of the disinfecting method and disinfecting apparatus according to the embodiment of the present invention, and the disinfectant using light according to the embodiment of the present invention are composed of an aqueous proanthocyanidin solution, It consists of a semiconductor laser capable of irradiating light with a wavelength of 405 nm.
- the qualitative quantitative analysis of the reactive oxygen species was performed by an electron spin resonance (ESR) spin trap method.
- ESR electron spin resonance
- DMPO 5,5-dimethyl-1-pyrroline N-oxide
- the obtained ESR spectrum is shown in FIG. Moreover, the production amount of each active oxygen was calculated
- PA proanthocyanidin concentration
- DMPO-OOH was produced at about 3.5 ⁇ M.
- the amount of DMPO-OOH produced decreased in a concentration-dependent manner, and it was confirmed that no DMPO-OOH was produced at a proanthocyanidin concentration of about 0.25 mg / mL. It was done.
- the IC50 of proanthocyanidins for DMPO-OOH production at this time was 0.005 mg / mL.
- a bactericidal test was performed. Streptococcus aureus ATCC 25923 was used as a bacterium, which was suspended in physiological saline and used in the test at a concentration of 2 ⁇ 10 7 cells / mL. 150 ⁇ L of the bacterial suspension and 150 ⁇ L of the proanthocyanidin aqueous solution were mixed in a microplate, and irradiated with a 405 nm laser at 300 mW (irradiance: 940 mW / cm 2 ) for 10 minutes.
- the final concentration of proanthocyanidins was 4 ⁇ g / mL to 3.2 mg / mL, and the influence of the concentration on the bactericidal effect was examined.
- 50 ⁇ L of the sample and 50 ⁇ L of 5000 U / mL catalase were mixed to stop the reaction of hydrogen peroxide derived from proanthocyanidins.
- a 10-fold dilution series was prepared, seeded on Brain Heart Infusion (BHI) agar medium, and cultured for 24 hours under aerobic conditions at 37 ° C. to determine the bactericidal effect.
- BHI Brain Heart Infusion
- the bactericidal effect of proanthocyanidins alone (1 mg / mL) and laser irradiation alone was also evaluated.
- FIG. 8 shows the test results with and without laser irradiation when the proanthocyanidin concentration is 1 mg / mL and without proanthocyanidin and laser irradiation.
- FIG. 9 shows the test results when laser irradiation was performed while changing the concentration of proanthocyanidins.
- the proanthocyanidin concentration was lower than 0.25 mg / mL and higher than 4 mg / mL, it was also confirmed that the bactericidal effect was attenuated. This is because of the relationship between the bactericidal effect of hydroxyl radicals produced by laser irradiation on proanthocyanidins and the antioxidant effect of excess proanthocyanidins, a high bactericidal effect can be obtained only in such a limited concentration range. It is done. In other words, when the concentration of proanthocyanidins is increased, catechins contained in the increasing proanthocyanidins first reduce dissolved oxygen to generate hydrogen peroxide. Since the hydroxyl radical is generated, the bactericidal effect is enhanced.
- the sterilization method, the sterilization apparatus, and the bactericide using light according to the embodiment of the present invention, the bactericidal effect by the hydroxyl radical generated by irradiating the bactericide with light is obtained, Can be sterilized. Moreover, the synergistic effect by the bactericidal action of catechins and the bactericidal action of a hydroxyl radical is acquired. Compared with the bactericidal action by catechins when not irradiating light with the generated hydroxyl radical, a higher bactericidal effect can be obtained in a short time. Proanthocyanidins containing catechins are more stable and less toxic than hydrogen peroxide, so that a stable sterilizing effect is obtained and safety is high compared to a sterilization method using hydrogen peroxide directly.
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Abstract
Description
ところが、図2に示すように、供与体として作用した後のフェノール性水酸基は、最終的にキノン構造となるため、ヒドロキシルラジカルを消去することはできない。このため、充分な溶存酸素存在下では、フェノール性水酸基は供与体として作用し、ヒドロキシルラジカルを消去しないため、ヒドロキシルラジカルによる殺菌効果が得られる。
このように、本発明者らは、通常は殺菌効果が得られるとは予測できない方法に殺菌効果を見出し、本発明に至ったものである。
カテキン類を含む殺菌剤は、カテキン類を含む溶液から成ることが好ましく、特にプロアントシアニジン水溶液から成ることが好ましい。さらに、前記プロアントシアニジン水溶液は、プロアントシアニジン濃度が0.25乃至4mg/mLであることが好ましい。プロアントシアニジンは、カテキンを複数個重合した物質であるため、特に高い殺菌効果を得ることができ、また、安全性も高い。
照射する光の放射照度は、 300 mW/cm2以上が好ましく、より大きいほど効果的である。
本発明の実施の形態の殺菌方法および殺菌装置の殺菌剤、ならびに、本発明の実施の形態の光を利用する殺菌剤は、プロアントシアニジン水溶液から成り、殺菌剤に光を照射する発光手段は、波長が405nmの光を照射可能な半導体レーザーから成っている。
field sweep:330.50-340.50mT
field modulation frequency:100kHz
field modulation width:0.05mT
amplitude:80
sweep time:2min
time constant:0.03s
microwave frequency:9.420GHz
microwave power:4mW
Claims (8)
- カテキン類を含む殺菌剤を殺菌対象に接触させた後、前記殺菌剤に光を照射することを、特徴とする殺菌方法。
- 前記カテキン類はガレート基を有していることを、特徴とする請求項1記載の殺菌方法。
- 前記殺菌剤はプロアントシアニジン水溶液から成ることを、特徴とする請求項1または2記載の殺菌方法。
- 前記プロアントシアニジン水溶液は、プロアントシアニジン濃度が0.25乃至4mg/mLであることを、特徴とする請求項3記載の殺菌方法。
- 前記光は、波長が350nm乃至500nmであることを、特徴とする請求項1、2、3または4記載の殺菌方法。
- プロアントシアニジン濃度が0.25乃至4mg/mLのプロアントシアニジン水溶液から成る殺菌剤と、
殺菌対象に接触させた前記殺菌剤に、350nm乃至500nmの波長を有する光を照射可能に設けられた発光手段とを、有することを特徴とする殺菌装置。 - プロアントシアニジン水溶液を含むことを、特徴とする光を利用する殺菌剤。
- プロアントシアニジン濃度が0.25乃至4mg/mLであることを、特徴とする請求項7記載の光を利用する殺菌剤。
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SG2013048905A SG191751A1 (en) | 2011-01-22 | 2011-12-01 | Disinfection method, disinfection device, and disinfecting agent utilizing light |
KR1020127032084A KR20140018773A (ko) | 2011-01-22 | 2011-12-01 | 살균 방법 및 살균 장치 |
EP11856576.1A EP2647286B1 (en) | 2011-01-22 | 2011-12-01 | Disinfection method, disinfection device, and disinfecting agent utilizing light |
US13/807,224 US8999236B2 (en) | 2011-01-22 | 2011-12-01 | Disinfection method and disinfection device |
JP2012553570A JP5265057B2 (ja) | 2011-01-22 | 2011-12-01 | 殺菌方法および殺菌装置 |
CN201180037822.1A CN103228138B (zh) | 2011-01-22 | 2011-12-01 | 一种杀菌方法及杀菌装置 |
BR112013004151A BR112013004151A2 (pt) | 2011-01-22 | 2011-12-01 | método de desinfecção e dispositivo de desinfecção |
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US8956156B2 (en) | 2013-03-08 | 2015-02-17 | A-Z Ltd. | Device for treating peri-implantitis |
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SG11201700815PA (en) * | 2014-08-12 | 2017-03-30 | Minoru Kanno | Cell repair agent and disinfection system |
CN107028175A (zh) * | 2016-02-03 | 2017-08-11 | 苏州工业园区新国大研究院 | 马来樱桃叶提取物、其制备方法及其在制备能量缓释的食品中的应用 |
CN113197227A (zh) * | 2021-03-24 | 2021-08-03 | 上海耐定工贸有限公司 | 一种用于牙刷杀菌盒的杀菌剂 |
WO2024130730A1 (zh) * | 2022-12-23 | 2024-06-27 | 中国科学院生态环境研究中心 | 可持续产生羟基自由基的方法、装置及应用 |
CN115956566B (zh) * | 2022-12-23 | 2024-09-06 | 中国科学院生态环境研究中心 | 一种持久性自由基材料及其制备方法与应用 |
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