WO2012093878A4 - Repellent composition for hematophagous arthropods - Google Patents

Abstract

The present invention relates to a repellent composition for hematophagous arthropods. The repellent composition comprises vanilline and one or two kinds of essential oils selected from the group consisting of Cinnamomum cassia, Rosmarinus officinalis, Eucalyptus citriodora, Cimbopogon citratus, Zanthoxylum armatum, and Cimbopogon nardus. Therefore, the repellency for hematophagous arthropods is improved and duration time of the repellency is increased. Additionally, the invention relates to a porous carrier containing the repellent composition for hematophagous arthropods, and a repellent-generating apparatus.

Description

Viscous Artificial Insect Repellent Compositions for Arthropods

More particularly, the present invention relates to a perspiration composition for an arthropod, which comprises a plant essential oil and vanillin, and is used as a vasculogenic arthropod for improving evapotranspiration against arteriosclerotic animals such as mosquitoes, To an animal repellent composition.

The vampire arthropods are very diverse, including mosquito, tick, fly (stable fly, tsetsefly), horse fly (bed fly) and bed bug. In particular, mosquitoes, which are the most important sites for blood-circulating arthropods, are the main mediators of malaria, including dengue fever, yellow fever, encephalitis and West Nile virus, affecting more than 300 million to 500 million people annually. Death is causing serious harm to more than 1 million people annually (Rozendaal, JA 1997. WHO Geneva, Switzerland.:http://www.who.int/water_sanitation_health / resources / vectorcontrol / en /).

Thus, a variety of repellents have been developed for vasodilated arthropods. These repellents are known to be the most effective means of preventing humans and livestock from vomiting by life-threatening insects (Curtis, CF, JD Lives, LU Baolib, and A. Renz 1990. Natural and synthetic repellents, 92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and increasingly regulated world. Annu. Rev. Entomol. 51: 45-66).

Despite its effectiveness, DEET (N, N-diethyl-3-methylbenzamide), the most widely used repellent, is known to have various adverse effects such as unpleasant odor, plastic or rubber damage, central nervous system depression, dermatitis induction, mammalian DNA damage (Katz, TM, JH Miller, and AA Hebert. 2008. Insect repellents: historical perspectives and new developments J. Am. Acad. Dermatol. 58: 865-871 .; Knowles, CO 1991. Miscellaneous pesticides, pp. 1471- 1526. In JB Hayes, Jr. and ER Laws, Jr. [eds.], Handbook of pesticide toxicology. Academic Press, San Diego, CA.). Thus, there is a need for safer types of repellents that can be prevented from blood-borne organisms.

In recent years, vegetable oil has been attracting attention as an alternative resource to develop natural repellents for blood-borne arthropods (Ahn, YJ, SI Kim, HK Kim, and JH Tak 2006. Naturally occurring house dust mites control et al., eds.), Naturally occurring bioactive compounds, Elsevier, London, United Kingdom .; Curtis, CF, JD Lives, LU Baolib, and A. Ren, 1990. Natural and synthetic repellents, pp. 75-92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Das, N. G., I. Baruah, P. K. Talukdar, and S. C. Das. 2003. Evaluation of botanicals as repellents against mosquitoes. J. Vector Borne Dis. 40: 49-53 .; Rozendaal, J. A. 1997. Vector control: methods for use by individuals and communities. World Health Organization, Geneva, Switzerland). Plant essential oils are useful for plants because they contain a variety of bioactive materials and have relatively little impact on the environment and organisms and are relatively inexpensive compared to plant extracts.

Accordingly, it is an object of the present invention to provide a bleeding agent composition for arthropods, which solves the problems of conventional synthetic repellent agents and improves the safety of the arthropods.

It is still another object of the present invention to provide a carrier and an apparatus for applying such a repellent composition.

In order to achieve the above object, the blood-sucking arthropod sensitive composition according to the present invention is characterized by comprising plant essential oil and vanillin.

In order to accomplish still another object, there is provided a porous carrier comprising a blood-sucking arthropod catheter composition according to the present invention.

According to another aspect of the present invention, there is provided an apparatus for generating an avian influenza agent for arthropod according to the present invention.

The blood-sucking arthropod detergent composition according to the present invention, the carrier and the detergent-containing generator comprising the same, improve not only the evasive force for the blood-circulating arthropods but also increase the duration thereof, and are safer than conventional repellents.

1 is a view showing a test cage used in the cage test method according to the present invention. The cage forms a frame with an iron frame, the material treatment zone 1 and the radiation cage 3 located at both ends are wrapped with a polyester net, and the centerless untreated zone 2 is wrapped with an acrylic plate. The holes in the polyester net are illustrative and not proportional to the picture, and are small enough that the mosquito can not escape.

2 is an illustration of a module comprising a bead preparation made in accordance with the present invention.

The blood-sucking arthropod detonator composition according to the present invention comprises plant essential oil and vanillin.

The blood-sucking arthropods to which the repellent composition of the present invention can be applied refers to all living things that take blood or body fluids from the skin of a mammal or bird, such as mosquitoes, insects, swallow flies, Flies, lobsters, lobsters, and the like.

The plant essential oil used in the present invention can be used without any particular limitation as long as it is known as a repellent for a vasodilated arthropod. Preferably it cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Zanthoxylum armatum ), Cymbopogon nardus , and the like. The plant essential oil of the present invention obtained from the above plants can be obtained by a cold precipitation method using a conventional solvent such as water, ethanol, acetone, methanol, propanol, ether, hexane, ethyl acetate, chloroform or a mixed solvent thereof, May be obtained by using one of the methods such as an immersion method, a hydrothermal extraction method (electronic method), a fermentation extraction method, a filtration leaching method, a supercritical fluid extraction method and the like, and more preferably, obtained by a steam distillation method. It may also be obtained from plant parts, such as roots, stems, leaves, flesh, flowers, fruits, seeds, and / or shells, respectively, or from whole plants.

The vanillin used in the present invention is 4-hydroxy-3-methoxybenzaldehyde as one of vanilla-flavored aromatic aldehydes. There is no particular restriction on the form and method of use, and a commercially available product can be purchased and used. On the other hand, various substances have been known in connection with the pleure agent for blood-circulating arthropods, but vanillin is not known as an insect repellent agent.

The blood-sucking arthropod sensitive compositions according to the present invention are suitable for use in the production of cinnamomum cassia , Rosmarinus officinalis , Eucalyptus citriodora , Cimbopogon citratus, ), Zanthoxylum armatum , and Cymbopogon nardus , and vanillin. The present invention also provides a method for producing a plant extract of the present invention. In this case, the composition ratio of plant essential oil to vanillin is not particularly limited, but it is preferable that the weight ratio of (1 to 3): (1 to 3) is in view of solubility in solvents and mutual mixing.

In addition, the perspirant composition for vodka arthropod according to the present invention may contain two plant essential oils and vanillin. In this case, there is no particular limitation on the composition ratio of each plant essential oil and vanillin, but considering the solubility in solvent, intermixability, and maximization of empowerment, plant essential oil: composition ratio of another plant essential oil: vanillin is ): (1 to 3): (1 to 3) (v / v / w). (V / v / w) of 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3.

The blood-sucking faecal composition for arthropod according to the present invention may be formulated by further containing suitable additives, excipients or carriers depending on the purpose of use and application environment. Examples of the emulsifier include emulsions, emulsifiers, wetting agents, water-soluble agents, aerosols, pump sprayers, suspensions, ULV (Ultra Low Volume) preparations, handmade, granules, powders, powders, granules, capsules, Beads, tablets, extracts, fluid extracts, lotions, mets, and the like. Preferably as a cream, pump spray or aerosol formulation.

The perspirant composition for vodka arthropod according to the present invention can be applied to a porous carrier for sustained release. The porous carrier used herein is not particularly limited, and examples thereof include charcoal, silica gel, diatomaceous earth, and the like. The porous carrier may be a gel formed to contain micropores by bonding between units constituting the polymer, or a viscopearl (Rengo, Tokyo, Japan) produced by pressing a polymer or polymer fiber . The biscarprins provide excellent porosity and are thus suitable for the gradual release of the blood-borne arthropod crainer compositions according to the invention.

The route of application of the blood-sucking arthropods composition according to the present invention may be, but is not limited to, direct dispersal or distribution of the repellent composition in the area where blood-borne arthropods predominantly occur, It can be used directly on the skin of birds, or it can be applied to daily necessities such as clothes, bags, bands, tents, and indoors.

When applied to a region where blood-borne arthropods are predominant, they can be applied directly or dispersed in the air. For example, when applied to a certain space, such as in a building, a porous carrier that continuously discharges the repellent composition can be mounted.

When used directly on the skin of mammals or birds, an appropriate amount can be applied or sprayed onto the skin. When used directly on skin, it should be extremely low to avoid irritation or skin problems.

When the capsule is applied to daily necessities, a capsule containing the repellent composition is prepared, and the repellent composition, which is supported when the capsule is rubbed by friction, is added to the air It is possible to use it so that it emits an eruptive power or it is directly mixed and then gradually released into the air. As such an example, a repellent composition according to the present invention may be supported on a porous carrier. At this time, if the indicator is included in the device for gradually discharging the repellent composition, if the repellent composition as an effective substance is completely volatized, the service life can be displayed by, for example, discoloration.

The blood-sucking faecal composition for arthropod according to the present invention can also be applied to household appliances such as air purifiers, air conditioners, and air conditioners. The application to such household appliances can be appropriately modified according to a conventional method. In the case of applying to the above-described household appliances, if the air purifier, the air conditioner, the air conditioner, or the like has a function of convecting the air inside the house, it is possible to cause the vapor permeable disposable diaper composition according to the present invention to dissipate in the purified air Especially useful.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the embodiments described below are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited to the following embodiments.

Example

1. Materials Preparation

Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), crooked pogon Carry Traders (Cimbopogon nardus) and vanillin Was purchased from Berje, Bloomfield, NJ, and Zanthoxylum armatum was purchased from Seema International, Deli, India. DEET was purchased from Sigma-Aldrich (St. Louis, Mo.).

One of the above-mentioned materials selected from plant essential oils and vanillin were dissolved in ethanol to prepare a composition. The plant essential oil and vanillin were mixed at a ratio of 5%: 5% (v / w) and 5%: 15% (v / w) respectively and dissolved in ethanol to obtain a final 100 μl of each composition.

On the other hand, ethanol solutions containing DEET instead of plant essential oil and vanillin were prepared as 5% (v) and 15% (v), respectively.

In addition, in order to prepare a composition containing two plant essential oils and vanillin, two different species were selected from Eucalyptus citriodora, Simbo fungus sheet rotus and Zanthoxylum armatum in plant essential oil, and ethanol was added to vanillin To prepare 100 mu l of each composition. 5%: 5%, 15%: 5%, and 5%: 1: 1: 1, : 5%, 5%: 15%: 5% and 5%: 5%: 15% (v / v / w). In addition, cymopogon ganoderma and zanthoxylum armatum and vanillin were prepared at a ratio of 8.5: 8.5: 3, with a ratio of eucalyptus citriodora, zanthoxylum armatum and vanillin of 8.5: 8.5: 3 (v / v / w).

2. Preparation of experimental insects

Ae . Aegypti for the test was induced by direct immersion in the mouse with adult insects exposed to insecticide for several years without any exposure to pesticides, resulting in 10% (w / v) sugar water To obtain insufficient carbohydrates. The larvae were placed in plastic trays (24 × 35 × 5 cm) to provide 0.5 g sterile artificial food (40-mesh cheese feed powder / yeast, 4: 1, w / w). These were kept at 26 ± 2 ° C and 70 ± 5% relative humidity, and the contrast was maintained at 16: 8 hours.

3. Experiment

end. Skin test method

In order to test the erectile force of the composition according to the present invention on mosquito adults, the composition obtained in the preparation of the material was uniformly applied to the surface of the skin of the tester exposed to a hole with a diameter of 5 cm in the back of a rubber glove to perform a skin test. Trial men were healthy 21-40 year old men. The control group treated only 100 占 퐇 of ethanol.

5-8 day old female mosquitoes were prepared in adult cages (25 × 25 × 25 ㎤) for mosquito rearing (250-260 individuals). When the control forearm was exposed for 1 minute and more than 6 subjects sat or had a blood-sucking behavior, it was judged that the blood pressure was enough and the evacuation test was conducted. The experiment was stopped when the forearms treated with the compositions were exposed for 3 minutes at intervals of 30 minutes to show that at least 2 consecutive vomiting behaviors such as sitting mosquitoes for at least 2 seconds, putting the snout on the skin, Complete protection time (CPT) was considered to be the point at which the original substance was treated and the time when more than 2 individuals sat on the treatment site continuously or had a vomiting action.

The complete avoidance duration was between the time of treatment and the time of the first blood vomiting, and a general linear analysis (GLM) was performed for the comparison between treatment averages. Bonferoni's test (P = 0.05) was used to compare the mean values.

The results are shown in Tables 1 to 2. Table 1 shows the complete repellency time (CPT) for the plant essential oils and the birds of Vanillin and plant essential oils and vanillin for the Egyptian mosquito adults. As controls, the complete repulsion duration (CPT) for the Egyptian mosquito adults of DEET alone is also described. Table 2 shows the results of complete repulsive time (CPT) for two species of plant essential oils and vanillin composition for the Egyptian mosquito adults.

Table 1

	Average Perfect Repulsive Duration (CPT) (range, minutes)
	Except vanillin		With vanillin
	5%	15%	5%: 5%	5%: 15%	15%: 5%	15%: 15%
Shin Nam Mo	75 (60-90)	- *	135 (120-150)	157.5 (120-180)	- *	- *
Rosmarinus officinalis	0 (0)	60 (60)	52.5 (30-60)	112.5 (60-150)	60 (30-90)	135 (120-150)
Eucalyptus citriodora	22.5 (0-30)	52.5 (30-60)	60 (60)	67.5 (30-120)	75 (60-90)	90 (60-120)
Shimbo Fogon Seattus	30 (30)	37.5 (30-60)	105 (90-120)	97.5 (60-120)	105 (90-120)	150 (150)
Zanthoxum Armatumum	30 (30)	75 (60-90)	60 (60)	90 (60-120)	75 (60-90)	120 (90-150)
Simbo Fogon Narders	25 (25)	70 (60-80)	55 (55)	85 (50-120)	70 (60-80)	105 (85-125)
vanillin	15 (0-30	37.5 (0-60)
DEET	127.5 (120-150)	247.5 (240-270)

^{* The} skin irritation is so strong that it can not be obtained

As shown in Table 1, the CPT was 75 minutes in the case of 5% Shinnamomukkasia alone, 30 minutes in the Simbo fogon sheet Latatus and Zanthoxylum Armatum, and 22.5 minutes in the Eucalyptus triiodora and vanillin, respectively. 15 minutes, Rosemary nasal opisinaris did not show any effect. On the other hand, DEET showed the strongest evasive force at 127.5 minutes and improved to 15% at 247.5 minutes. On the other hand, as can be seen from the above Table 1, it can be seen that the composition including the plant essential oil and vanillin improves the synergistic effect as compared with the case of using each alone. The enhancement of these evoked potentials was confirmed by the fact that the CPT increased sharply (F = 71.75, P <0.0001) when vanillin was mixed with 5% and 15% of each plant essential oil. Thus, the two factors, plant concentration and vanillin mixing, were found to be significant for the epigraph of the mosquito (F = 5.61, P <0.0001).

On the other hand, a repellent composition according to the present invention was prepared by mixing two plant essential oils and vanillin, and the same experiment was carried out. The results are shown in Table 2.

Table 2

Group a	CPT average (range, minutes)
	1: 1: 1	3: 1: 1	1: 3: 1	1: 1: 3
A	105 (90-120)	165 (150-180)	165 (120-210)	165 (150-180)
B	142.5 (90-180)	82.5 (60-120)	150 (150)	240 (210-300)
C	150 (120-180)	210 (180-240)	270 (210-330)	240 (180-300)
D	140 (110-170)	200 (170-230)	240 (180-300)	220 (150-290)

^a Group A: Eucalyptus Citrillo Dora: Symbol Fogon Sheet Latous: Vanillin

Group B is Eucalyptus citriodora: Zanthoxum Armatumum: Vanillin

Group C is Shimbo Fogon Sheet Latous: Zanthoxum Armatumum: Vanillin

Group D is Shimbo Fogon Narders: Zanthox Samrum Armatum: Vanillin

As can be seen in Table 2 above, the two plant essential oils and the vanillin blend exhibited far superior evacuation (CPT) than the single oil and vanillin blend. It can be seen that this effect produces synergies more than the increase in repelling duration by simple mixing of the constituents of these compositions. That is, the 1: 1: 1 standard values of the composition components obtained from the results of Table 2 were compared based on the CPT. 15 minutes, the experimental results obtained from the actual composition of Table 2 above are about 105 times more than the expected value, which is about twice the expected value. That is, it was confirmed that the detergent composition for vampire arthropod according to the present invention has a synergistic effect due to mixing of two plant essential oils and vanillin.

I. Cage test method

This test is intended to test whether mosquitoes enter the treatment area through a thick cardboard (building window) with a 10 cm hole in the middle, lying as a wall in the center of the test system. In addition, this test is intended to test an average blocking power of the bead formulation over time.

In this test, 5 mu l of the composition according to the present invention was treated with a filter paper or a bead agent was prepared and used. The bead agent was prepared by impregnating the viscous agent composition according to the present invention with biscotti (3.8 to 4.2 mm, Rengo, Tokyo, Japan) which is a porous cellulose bead. In the first g of the obtained biscottulbide obtained, the composition was about 0.465 g and the weight of biscotti pear was about 0.535 g. The ratios between the respective components of the composition used are summarized in the following table.

To carry out the cage test, 100 female mosquitoes of the Egyptian mosquito which had been adult for 5-10 days were used and prepared 2 hours before the start of the test. The test cage is illustrated in Fig. The cage comprises a material frame 1, a non-treatment zone 2 and a radiation cage 3 with steel frames, the material treatment zone 1 and the radiation cage 3 are wrapped with a polyester net, (2) is an acrylic plate. In the center of the untreated zone (2), a thick cardboard was drilled with a 10 cm diameter hole. The mouth and the bead treated with the repellent composition according to the present invention were placed in the material processing zone 1 before the start of the test so that they could not be immobilized by immobilizing one mouse as a mosquito ape in the material processing zone 1, . In the case of the wastes treated with the composition, the wastes were naturally dried for 1 minute, fixed to the upper end of the material treatment zone 1 by spatting and allowed to volatilize for 3 minutes. Thereafter, the adult female mosquitoes of the Egyptian forest prepared in the spinning cage 3 were spun. In this test, the natural movement flow of the mosquito is the material treatment zone (1) from the mosquito radiation cage (3).

The average rate of blocking the entry of mosquitoes into the material treatment zone 1 was calculated using the following equation:

(%) = ((30 - Number of mosquitoes infiltrating the treatment area) / 30} × 100

The results are shown in Table 3.

Table 3

		Average Blocking Rate (%)
		30 minutes	60 minutes	90 minutes	120 minutes
A: B: D = 1: 1: 1	5 mu l of the composition was treated with a filter paper	90	84	84	80
A: C: D = 1: 1: 1		95	92	92	84
B: C: D = 1: 1: 1		92	92	92	89
A: B: D = 3: 1: 1		96	96	91	92
A: C: D = 3: 1: 1		84	80	80	72
B: C: D = 3: 1: 1		100	98	98	96
A: B: D = 1: 3: 1		96	94	94	92
A: C: D = 1: 3: 1		95	94	92	92
B: C: D = 1: 3: 1		100	98	98	98
A: B: D = 1: 1: 3		92	92	86	82
A: C: D = 1: 1: 3		100	100	99	98
B: C: D = 1: 1: 3		100	98	98	96
A: B: D = 1: 1: 1	Treated with biscotti 1 g of biscotti pearl	92	91	84	84
A: C: D = 1: 1: 1		96	94	89	84
B: C: D = 1: 1: 1		96	92	90	90
A: B: D = 3: 1: 1		92	92	88	81
A: C: D = 3: 1: 1		89	81	77	73
B: C: D = 3: 1: 1		98	98	91	88
A: B: D = 1: 3: 1		89	84	84	78
A: C: D = 1: 3: 1		91	84	84	81
B: C: D = 1: 3: 1		100	94	91	90
A: B: D = 1: 1: 3		94	92	87	82
A: C: D = 1: 1: 3		98	98	94	91
B: C: D = 1: 1: 3		100	96	96	94

A = Eucalyptus citriodora

B = Symbol Fogon Seat Latus

C = Zotoxylum Armatum

D = vanillin

As shown in Table 3, the composition prepared in the composition ratio shown in Table 3 was directly treated on the foliar paper to examine the mosquito inflow inhibiting power into the treatment area. As a result, it was found that after 120 minutes from the test, the eucalyptus citriodora: At a ratio of 3: 1: 1, was able to inhibit penetration of 80% or more of mosquitoes except for the case where the composition was treated in a foliar condition.

In addition, even when the composition prepared in the composition ratio shown in Table 3 was treated on the bismuth pearl (size: 3.8 to 4.2 mm, Rengo, Tokyo, Japan) The effect of inhibiting penetration was shown. Therefore, when the active ingredient of the composition according to the present invention is distributed in a space above a certain concentration, the mosquito is recognized by the mosquito, thereby avoiding infiltration into the space where the avoidant material is exposed.

All. Chamber test

A chamber test was conducted to determine the erodibility of the composition of the present invention in a large space. The chamber (1.2 X 1.2 X 1.2 m) used for the test was made of an iron plate covered with an acrylic plate and a square hole (20 X 20 cm) was installed at the bottom of the front. The square hole was closed with a black cardboard with ten holes (each hole 1.5 x 1.5 cm). A mosquito radiation cage (25 x 25 x 25 cm) was connected to the square hole. Another collection cage was placed in the chamber. The spinning cage and the collection cage were connected by a card board, all wrapped in a polyester net. A fan (model name: EFB-2-3, Hanil, Korea) was operated to promote the diffusion of components. One human volunteer as a mosquito ape entered the chamber. As a control group, there were no repellents and only mosquito apes were present. Count the number of mosquitoes entering the chamber for a period of time. A module containing a bead agent described below was used as the avoidance agent. The manure treatment was carried out 30 minutes before the test, and the repellent treatment was carried out 1 hour before the test. Repeated tests were conducted to obtain the results through statistical processing.

The bead agent is the same as that used in the cage test method. The composition to be supported on the bead agent is a composition (using ethanol as a solvent) prepared by mixing Simpol Vorton sheet LatouszanthoxamineArmatum: vanillin at a ratio of 8.5: 8.5: 3 (v / v / w).

On the other hand, a module having the shape shown in FIG. 2 was manufactured for this experiment. Ten holes are formed in the module to control opening and closing and each of the holes contains the same amount of beads so that a total of about 10 to 11 g of beads is contained in one module, To 5.4 g of the composition is carried.

120 sets of Egyptian female female mosquitoes were used, from which the set of 2 modules with 10 openings in the chamber was placed in the above-mentioned chamber and the adult was 5 to 10 days old. The number of female mosquitoes entering the chamber was counted at 5, 10, 20 and 30 minutes after fan operation. The eradication rate was calculated by counting the number of incoming mosquitoes (control group) and the number of incoming mosquitoes at the time of treatment of the composition of the present invention, and calculating the elimination rate at that rate. . The unit is percent (%).

The avoidance rate over time was repeated three times to obtain an average avoidance rate. The results of the eruptive force obtained are shown in Table 4.

The results of the eruptive force obtained are shown in Table 4.

Table 4

Repulsive force (mean ± SE)
No.	5 minutes	10 minutes	20 minutes
One	66.32 + - 11.35	77.73 + - 8.89	67.81 + - 12.83
2	100 ± 2.1	81.94 + 1.13	80.56 ± 13.71
3	76.36 + - 8.18	64.22 + - 5.87	64.41 + - 4.50
4	80.56 + - 10.02	55.99 + - 13.04	62.15 + - 8.22

1. Symbol Fogon Nardus: Zanthocum Armum Artumum: Vanillin = 8.5: 8.5: 3 (v / v / w)

2. Symbolic fugon sheet Latous: Zanthox sumum arthurmum: vanillin = 8.5: 8.5: 3 (v / v / w)

3. Simbo Fogon Nardus: Zanthocum Arumatumum: Vanillin = 1: 1: 1 (v / v / w)

4. Symbolic fugon sheet Latous: Zanthox sumumarumatum: vanillin = 1: 1: 1 (v / v / w)

It can be seen from the above Table 4 that the composition of the present invention having an excellent repellency of the mosquito repellent according to the present invention in a wide space and in particular a sympathetic fomentate sheet Latouszanthoxylumarmatumum vanillin = 8.5: 8.5: 3 (v / v / w) It can be confirmed that it is excellent in releasing force.

On the other hand, in order to confirm the avoidance rate and the service life of the composition of Simbo fugon sheet Latatus: Zanthoxylum arthammum: vanillin = 8.5: 8.5: 3 (v / v / w) The chamber test was performed and observed. The results obtained are shown in Table 5 below.

Table 5

Number of repeats	Time (minutes) after processing
	10 minutes	30 minutes	60 minutes
One	100	93.3	93.3
2	87.5	72.7	-
3	100	66.7	70
	(%, Mean ± SE)
Average	95.8 ± 4.2	77.6 ± 8	81.7 ± 11.7

As can be seen from the above Table 5, it can be seen that the total rejection rate is 83% or more and the rejection rate is 70% or more for a minimum of 60 minutes.

la. Accelerated test

The sustainability period of the ephemeris of the blood-borne arthropod disposable composition according to the present invention was measured by an accelerated test. The blood-borne arthropod disposable detergent composition is used on an average of about 8 hours a day. The number of holes through which mosquitoes can move on the walls of the chamber used in the chamber test is 10, And the number of holes in the wall of the inner wall was 40. In order to see if the persistence of the blood-borne arthropod craving composition according to the present invention lasts for 90 days, the effect of the repulsive force over time after running for 8 days under the above conditions was measured.

The test was carried out using the same chamber as the above chamber test except that the holes were increased to 40, the control group was treated with only the ethanol solvent, and the experimental group was treated with Simbo fogon sheet Latous: Zanthoxylum armatum: vanillin = 8.5: 8.5 : 3 (v / v / w) (the solvent is ethanol). The results obtained are shown in Table 6 below.

Table 6

inflow	Early	10 minutes	40 minutes	50 minutes	60 minutes	Average
Control group (Marie)	0	12	13	14	12	12.75
Experimental group (horses)	0	5	7	4	4	5
Emphasis (%)	0	58.3	46.2	71.4	66.7	60.8

From the above Table 6, it was confirmed that an average of 60% or more of the avoidance effect lasted for 90 days or more during the treatment of beads containing the composition according to the present invention.

Claims (9)

1. Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Xanthoxylum armatum) and crooked A plant essential oil selected from the group consisting of Cymbopogon nardus , and vanillin.
2. The method according to claim 1,

   Wherein the composition of plant essential oil and vanillin is (1 to 3): (1 to 3) (v / w).
3. Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Xanthoxylum armatum) and crooked A plant essential oil selected from the group consisting of Cymbopogon nardus and vanillin.
4. The method of claim 3,

   Plant Essential Oil: Another plant essential oil: a perspirant composition for vampire arthropod wherein the composition of vanillin is (1 to 3): (1 to 3): (1 to 3) ratio (v / v / w)
5. 5. The method of claim 4,

   (V / v / w) of a plant essential oil: vanillin composition of 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: A perspiration composition for a humoral blood circulating arthropod.
6. The method of claim 3,

   Xanthoxylum armatum , Cimbopogon citratus , and vanillin. &Lt; Desc / Clms Page number 20 &gt;
7. The method of claim 3,

   A perspirant composition for vampire arthropod comprising Xanthoxylum armatum , Cymbopogon nardus and vanillin.
8. A porous carrier comprising a blood-sucking arthropod catheter composition according to any one of claims 1 to 7.
9. 8. A detergent generator comprising a blood-sucking arthropod herbicidal composition according to any one of claims 1 to 7.

Images