WO2012093878A2

WO2012093878A2 - Repellent composition for hematophagous arthropods

Info

Publication number: WO2012093878A2
Application number: PCT/KR2012/000133
Authority: WO
Inventors: 이성화; 안용준; 권형욱; 박형호; 김순일
Original assignee: 엘지전자 주식회사; 서울대학교 산학협력단
Priority date: 2011-01-06
Filing date: 2012-01-06
Publication date: 2012-07-12
Also published as: WO2012093878A4; WO2012093878A3

Abstract

The present invention relates to a repellent composition for hematophagous arthropods. The repellent composition comprises vanilline and one or two kinds of essential oils selected from the group consisting of Cinnamomum cassia, Rosmarinus officinalis, Eucalyptus citriodora, Cimbopogon citratus, Zanthoxylum armatum, and Cimbopogon nardus. Therefore, the repellency for hematophagous arthropods is improved and duration time of the repellency is increased. Additionally, the invention relates to a porous carrier containing the repellent composition for hematophagous arthropods, and a repellent-generating apparatus.

Description

Blood repellent arthropod repellent composition

The present invention relates to a repellent composition for blood-sucking arthropods, and more particularly, to a blood-sucking arthropod that improves repelling power and increases the duration of repelling power for blood-sucking arthropods including plant essential oils and vanillin. A repellent composition for animals.

Vampire arthropods vary from mosquitoes to ticks, stable fly, tsetsefly, horse fly, and bed bugs. In particular, mosquitoes, which are the most important blood-sucking arthropods, are the major mediators of malaria, including dengue fever, yellow fever, encephalitis and West Nile virus, affecting more than 3-5 billion people a year. The death toll alone causes serious harm of more than 1 million people per year (Rozendaal, JA 1997. Vector control: methods for use by individuals and communities.WHO.Geneva, Switzerland.:http://www.who.int/water_sanitation_health / resources / vectorcontrol / en /).

Thus, various repellents have been developed for vampire arthropods. Such repellents are known to be the most effective means of preventing humans and livestock from vampires by harmful pests (Curtis, CF, JD Lives, LU Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75- 92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and increasingly regulated world. Annu. Rev. Entomol. 51: 45-66).

However, DEET (N, N-diethyl-3-methylbenzamide), which is the most widely used repellent, is known to have various side effects such as unpleasant odor, plastic or rubber damage, central nervous system suppression, dermatitis, and mammalian DNA damage. (Katz, TM, JH Miller, and AA Hebert. 2008. Insect repellents: historical perspectives and new developments. J. Am. Acad. Dermatol. 58: 865-871 .; Knowles, CO 1991. Miscellaneous pesticides, pp. 1471- 1526. In JB Hayes, Jr. and ER Laws, Jr. [eds.], Handbook of pesticide toxicology.Academic Press, San Diego, CA. Thus, there is a need for safer forms of repellent that can be prevented from vampires.

Recently, plant essential oils have attracted attention as an alternative resource for developing natural repellents for vampire arthropods (Ahn, YJ, SI Kim, HK Kim, and JH Tak. 2006. Naturally occurring house dust mites control agents: development and commercialization, pp. 269-289.In M. Rai and MC Carpinella [eds.], Naturally occurring bioactive compounds.Elsevier, London, United Kingdom .; Curtis, CF, JD Lives, LU Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75-92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Das, N. G., I. Baruah, P. K. Talukdar, and S. C. Das. 2003. Evaluation of botanicals as repellents against mosquitoes. J. Vector Borne Dis. 40: 49-53; Rozendaal, J. A. 1997. Vector control: methods for use by individuals and communities. World Health Organization, Geneva, Switzerland). Plant essential oils are useful because plants contain a variety of biologically active substances and have relatively little effect on the environment and organisms and are also relatively inexpensive compared to plant extracts.

Therefore, an object of the present invention is to solve the problems of the conventional synthetic repellent, to provide a safer and more repellent for vampire arthropods and to provide a repellent composition for vampire arthropods to increase the duration of the repellent force.

It is another object of the present invention to provide a carrier and a generating device to which such a repellent composition is applied.

In order to achieve the above object, the repellent composition for blood-sucking arthropods according to the present invention is characterized in that it comprises plant essential oils and vanillin.

In order to achieve another object, it provides a porous carrier comprising a repellent composition for blood-absorbing arthropods according to the present invention.

In addition, in order to achieve another object, it characterized in that it comprises a repellent generating device for blood-sucking arthropod according to the present invention.

The repellent composition for blood-sucking arthropod according to the present invention and the carrier and repellent generating device including the same improve the repelling force and increase the duration of the blood-sucking arthropod, and are safer than conventional repellents.

BRIEF DESCRIPTION OF THE DRAWINGS The figure which shows the test cage used for the cage test method which concerns on this invention. The cage is framed by an iron frame, the material handling zones 1 and the spinning cage 3 located at both ends are wrapped in polyester nets, and the center untreated zone 2 is wrapped with acrylic plates. The holes in the polyester net are examples only and are not proportional to the figure and are small enough to prevent mosquitoes from escaping.

2 is an illustration of a module comprising a bead made according to the present invention.

The repellent composition for blood-sucking arthropods according to the present invention includes plant essential oils and vanillin.

The blood-sucking arthropod to which the repellent composition of the present invention can be applied refers to all living organisms that take blood or body fluids from the skin of mammals or birds, for example, vampires of mosquitoes, insects, chimneys, fly flies, and the like. Flies, baboons, stools, and the like.

The plant essential oils used in the present invention can be used without particular limitation as long as they are known as repellents for vampire arthropods. Preferably it cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Zanthoxylum armatum ) And cymbopogon nardus . The plant essential oil of the present invention is obtained from the above-mentioned plants, and is cooled by a conventional solvent (eg, water, ethanol, acetone, methanol, propanol, ether, hexane, ethyl acetate, chloroform or a mixed solvent thereof), warm It may be obtained by using one of methods such as immersion method, hot water extraction method (electronic method) and fermentation extraction method, filtration leaching method, supercritical fluid extraction method, and more preferably may be obtained by steam distillation method. In addition, it may be obtained from each plant part, for example, roots, stems, leaves, pulp, flowers, fruits, seeds, and / or bark, or the whole plant.

Vanillin used in the present invention is 4-hydroxy-3-methoxy benzaldehyde as one of the vanilla-smelling aromatic aldehydes. There are no particular restrictions on the form or method of use, and commercially available products can be purchased and used. Meanwhile, various substances have been known in relation to blood repellent arthropods, but vanillin is not known as an insect repellent.

The repellent composition for blood-sucking arthropods according to the present invention is Cinnamomum cassia , Rosmarinus officinalis , Eucalyptus citriodora , Cimbopogon citratus ), Zanthoxylum armatum and Cymbopogon nardus , one of the plant essential oils selected from the group consisting of vanillin. At this time, the composition ratio of plant essential oil and vanillin is not particularly limited, but a weight ratio of (1 to 3) to (1 to 3) is preferable in view of solubility in solvents and mutual mixing.

In addition, the repellent composition for blood-sucking arthropods according to the present invention may include two kinds of plant essential oils and vanillin. At this time, the composition ratio of each plant essential oil and vanillin is not particularly limited, but the ratio of plant essential oil: another type of plant essential oil: vanillin in consideration of maximization of solubility in solvents, mutual mixing, and avoidance is (1 to 3). It is preferable to set it as ratio (v / v / w) of (1-3): (1-3). More preferably, it is the ratio (v / v / w) of 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3.

The blood-absorbing arthropod repellent composition according to the present invention may be formulated further comprising suitable additives, excipients or carriers depending on the intended use or application environment. Examples include emulsions, emulsifiers, hydrating agents, water soluble agents, aerosols, pump sprays, suspensions, ULV formulations, fragrances, granules, powders, powders, granules, capsules, ointments, creams, It may be formulated into a bead, tablet, extract, fluid extract, lotion, met, and the like. It is preferably applied as cream, pump spray or aerosol.

In addition, the repellent composition for blood-absorbing arthropods according to the present invention can be applied to a porous carrier for sustained release. The porous carrier used at this time is not particularly limited, for example charcoal (silco gel), silica gel (silica gel), diatomaceous earth (diatomearth) and the like. The porous carrier is a gel (gel) formed to include a micropore as a bond between the units constituting the polymer, or biscopearl (viscopearl, Rengo, Tokyo, Japan), etc. produced by compressing the polymer or polymer fiber It includes. Since the biscopearl provides excellent porosity, it is suitable for the gradual release of the repellent composition for vampire arthropods according to the present invention.

The route of application of the repellent composition for blood-sucking arthropods according to the present invention is not limited thereto, but the dispersant composition may be directly dispersed or dispersed in areas where blood-sucking arthropods occur, or mammals including humans or domestic animals or It can be used directly on the skin of birds, or can be applied to household items such as clothes, bags, bands, tents, and indoors.

In areas where vampire arthropods are predominantly occurring, they can be distributed or dispersed directly in the air. Or, for example, when applied to a certain space, such as in a building, it may be subjected to a porous carrier that continuously releases the repellent composition.

When used directly on the skin of mammals or birds, an appropriate amount may be applied or sprayed on the skin. If used directly on the skin, it should be extremely small, irritating or even if it does not cause skin problems.

In addition, when applied to household goods, a capsule carrying the repellent composition is prepared, and the repellent composition carried in the air when the capsule is bursted by friction by manufacturing the cloth, cloth, and band, which is a material of the household goods, or directly incorporating into plastics. It can be used by being diverted to show repelling power or being directly mixed and then slowly released into the air. As such an example, the repellent composition according to the present invention may be supported on a porous carrier. In this case, an indicator (indicator) is included in the device for gradually releasing the repellent composition, so that when the repellent composition, which is an active substance, is all volatilized, for example, the service life may be displayed by discoloration.

The blood repellent arthropod repellent composition according to the present invention can also be applied to household appliances such as an air purifier, an air conditioner, and an air conditioner. Application to such household electrical appliances can be appropriately modified in accordance with conventional methods. Application to the above-mentioned home appliances, such as an air purifier, air conditioner, air conditioner, if it has the function to convection the air inside the house, it is possible to make the repellent composition for vampire arthropod repellent according to the present invention to the purified air Especially useful.

Hereinafter, the present invention will be described with reference to preferred embodiments. However, the examples described below are merely provided to more easily understand the present invention, and the present invention is not limited to the following examples.

실시예 Example

1. Material Preparation

Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), crooked pogon Carry Traders (Cimbopogon nardus) and vanillin Was purchased from Berje, Bloomfield, NJ, and Zanthoxylum armatum was purchased from Seema International, Deli, India. And DEET were purchased from Sigma-Aldrich (St. Louis, Mo.).

Of the above materials, one selected from plant essential oil and vanillin, respectively, was dissolved in ethanol to prepare a composition. Plant essential oil and vanillin were respectively mixed at a ratio of 5%: 5% (v / w) and 5%: 15% (v / w), and dissolved in ethanol to obtain a final 100 μl of the composition, respectively.

Meanwhile, 5% (v) and 15% (v) of ethanol solutions containing DEET instead of plant essential oil and vanillin were prepared for control.

In addition, in order to prepare a composition comprising two plant essential oils and vanillin, two different plants from eucalyptus citridora, cymbopogon citratus, and xanthoxylum armatum are selected from plant essential oils in ethanol together with vanillin. Each 100 μl of the composition was prepared by melting. The mixing ratios of the two plant essential oils and vanillin were 1: 1: 1, 3: 1: 1, 1: 3: 1 and 1: 1: 3, respectively, which were 5%: 5%: 5% and 15%: 5%. Same as the 5%, 5%: 15%: 5%, and 5%: 5%: 15% (v / v / w) ratios. Furthermore, cymbopogon Narders and Xantoxilum Armatum and Vanillin were prepared in a ratio of 8.5: 8.5: 3, and Eucalyptus citridora, Xantoxilum Armatum and Vanillin were prepared in a ratio of 8.5: 8.5: 3 (v / v / w).

2. Experimental insect preparation

For use in testing, Egyptian mosquitoes ( Ae . Aegypti ) were passaged directly to mice with adult insects that had been passaged indoors for years without any pesticide exposure to induce spawning and provide 10% (w / v) sugar water. To get enough carbohydrates. The larvae were placed in a plastic tray (24 × 35 × 5 cm) to provide 0.5 g of sterile artificial feed (40-mesh fry powder / yeast, 4: 1, w / w). These were maintained at 26 ± 2 ° C. and relative humidity of 70 ± 5% with contrast as 16: 8 hours.

3. Experiment

end. Skin test

In order to test the repellency of the composition according to the present invention for mosquitoes adults, the skin test was carried out by uniformly treating the skin surface of the tester exposed to the back of the rubber glove hand with a hole of 5 cm diameter in the preparation of the material. The test subjects were healthy 21-40 year old men. The control group treated only 100 μl of ethanol.

5-8 days old Egyptian mosquito female adults were prepared in 250-260 individuals in mosquito adult cages (25 × 25 × 25 cm 3) 12-24 hours before the start of the experiment. When the control forearm was exposed for 1 minute or more than 6 individuals sitting or exhibiting vampire behavior, it was judged as sufficient biting pressure and the evasion force test was performed. The experiment was discontinued if the forearm treated with the compositions was exposed for 3 minutes at intervals of 30 minutes and the adult mosquitoes were sitting for at least 2 seconds, or if more than two blood-sucking behaviors such as a snout was placed on the skin or a blood-sucking spot were found in succession. The complete protection time (CPT) was regarded as the time when the first material was treated and when two or more individuals sat in the treatment area or showed vampire behavior in succession.

The complete avoidance duration was defined as the time between treatment and the time of initial vampires. A general linear analysis (GLM) was performed for comparisons between treatment averages. Inter-mean comparisons were made using Bonferoni's test (P = 0.05).

The results are shown in Tables 1-2. Table 1 shows the complete avoidance duration (CPT) for Egyptian mosquitoes adults of plant essential oils and vanillin and a composition of one plant essential oil and vanillin. As a control, complete evacuation duration (CPT) is described together for Egyptian adult mosquitoes in DEET alone. Table 2 shows the results for the complete avoidance duration (CPT) for the adult Egyptian mosquitoes in the composition of two plant essential oils and vanillin.

Table 1

	Average Perfect Evasion Duration (CPT) (Range, Minutes)
	Except vanillin		Contains vanillin
	5%	15%	5%: 5%	5%: 15%	15%: 5%	15%: 15%
Cinnamomum Cassia	75 (60-90)	- ^*	135 (120-150)	157.5 (120-180)	- ^*	- ^*
Rose Mariners Officinalis	0 (0)	60 (60)	52.5 (30-60)	112.5 (60-150)	60 (30-90)	135 (120-150)
Eucalyptus citrudora	22.5 (0-30)	52.5 (30-60)	60 (60)	67.5 (30-120)	75 (60-90)	90 (60-120)
Cympogon Cilatus	30 (30)	37.5 (30-60)	105 (90-120)	97.5 (60-120)	105 (90-120)	150 (150)
Xantoxilum Armatum	30 (30)	75 (60-90)	60 (60)	90 (60-120)	75 (60-90)	120 (90-150)
Symbogongon Narders	25 (25)	70 (60-80)	55 (55)	85 (50-120)	70 (60-80)	105 (85-125)
vanillin	15 (0-30_	37.5 (0-60)
DEET	127.5 (120-150)	247.5 (240-270)

^* Due to strong skin irritation, experimental value cannot be obtained

As can be seen in Table 1, Cinnamomum cassia 5% alone treatment showed a 75-minute CPT, cymbopogon citratus and xantoxilum armatum 30 minutes and eucalyptus citridora and vanillin 22.5 minutes and At 15 minutes, Rosemarinus Offinalis had no effect. On the other hand, DEET showed the strongest evasion at 127.5 minutes and improved to 247.5 minutes at 15%. On the other hand, as can be seen in Table 1, the composition containing the plant essential oil and vanillin, it was found that the repelling power is improved while having a synergistic effect compared to when using each alone. This increase in repellency was shown by the fact that CPT increased rapidly when vanillin was mixed with 5% and 15% of each plant essential oil (F = 71.75, P <0.0001). Thus, two factors, the concentration of plant essential oil and vanillin mixture, were found to be significantly important for the repellent force of Egyptian forest mosquitoes (F = 5.61, P <0.0001).

Meanwhile, two plant essential oils and vanillin were mixed to prepare a repellent composition according to the present invention, and the same experiment was conducted. The results are shown in Table 2.

TABLE 2

Group ^a	CPT average (range, minutes)
Group ^a	1: 1: 1	3: 1: 1	1: 3: 1	1: 1: 3
A	105 (90-120)	165 (150-180)	165 (120-210)	165 (150-180)
B	142.5 (90-180)	82.5 (60-120)	150 (150)	240 (210-300)
C	150 (120-180)	210 (180-240)	270 (210-330)	240 (180-300)
D	140 (110-170)	200 (170-230)	240 (180-300)	220 (150-290)

A is ^a group of eucalyptus Rio Dora Tooth sheet: pogon Citra crooked tooth: vanillin

Group B is Eucalyptus Citridora: Xantoxilum Armatum: Vanillin

Group C is Cymbopogon Cilatus: Xantoxilum Armatum: Vanillin

Group D is Simbopogon Narders: Xantoxilum Armatum: Vanillin

As can be seen in Table 2, the two plant essential oils and vanillin blends showed significantly better repellency (perfect evacuation duration, CPT) compared to a single oil and vanillin blend. It can be seen that this effect results in synergies beyond that of increased repellency duration by simple mixing of each component of these compositions. That is, when comparing the 1: 1: 1 reference value of each composition component obtained from the results of Table 2 based on CPT, Cympogon citratus belonging to group A is 30 minutes, 22.5 minutes eucalyptus citriodor, and vanillin Arithmetic summation of these at 15 minutes can only expect 67.5 minutes, but the experimental results obtained with the actual composition of Table 2 can be seen that 105 minutes, which is about 2 times higher than expected. That is, the blood repellent arthropod repellent composition according to the present invention was confirmed to have a synergistic effect due to the mixing of two plant essential oils and vanillin.

I. Cage test method

This test is to test whether mosquitoes invade the treatment area through a thick card board (building window) that is laid horizontally in the center of the test system and has a 10 cm hole in the center. In addition, this test is to assay the average stopping power over time of the bead formulation.

In this test, the compositions according to the invention were treated with 5 μl as needed or prepared beads. The bead was prepared by supporting the repellent composition according to the present invention in a biscopearl (3.8-4.2 mm, Rengo, Tokyo, Japan), which is a porous cellulose bead. In 1 g of the biscopearl bead agent obtained, the composition was about 0.465 g, and the weight of the biscopearl was about 0.535 g. The ratio between each component of the composition used is summarized in the following table.

To perform the cage test, 100 female Egyptian mosquitoes, 5-10 days old, were used and prepared 2 hours before the start of the test. The test cage is illustrated in FIG. 1. The cage is made of a steel frame to produce a material treatment zone (1), an untreated zone (2) and a spinning cage (3), the material handling zone (1) and a spinning cage (3) are wrapped in a polyester net, and the untreated zone (2) put an acrylic plate. In the center of the non-treatment zone 2, a thick card board having a hole of 10 cm in diameter was installed. 10 minutes before the start of the test so that one mouse as a mosquito ape with a mosquito ape is immobilized in the material processing zone 1, and the room for treating the repellent composition according to the present invention and the bead agent are placed in the material processing zone (1). Put on. For the case where the composition was treated, it was allowed to dry naturally for 1 minute and suspended by thread on top of the material treatment zone 1 and allowed to volatilize for 3 minutes. Thereafter, female adult female mosquitoes in the spinning cage 3 were spun. The natural flow of mosquitoes in this test is the material handling zone (1) from the mosquito spinning cage (3).

The average rate of inhibition for preventing the ingress of mosquitoes into the material treatment zone 1 was calculated using the following formula.

% Blockage = {(Mosquitoes penetrated into 30-treatment zone) / 30} × 100

The results are shown in Table 3.

TABLE 3

		Average Block Rate (%)
		30 minutes	60 minutes	90 minutes	120 minutes
A: B: D = 1: 1: 1	5 μl of composition is treated here	90	84	84	80
A: C: D = 1: 1: 1		95	92	92	84
B: C: D = 1: 1: 1		92	92	92	89
A: B: D = 3: 1: 1		96	96	91	92
A: C: D = 3: 1: 1		84	80	80	72
B: C: D = 3: 1: 1		100	98	98	96
A: B: D = 1: 3: 1		96	94	94	92
A: C: D = 1: 3: 1		95	94	92	92
B: C: D = 1: 3: 1		100	98	98	98
A: B: D = 1: 1: 3		92	92	86	82
A: C: D = 1: 1: 3		100	100	99	98
B: C: D = 1: 1: 3		100	98	98	96
A: B: D = 1: 1: 1	Manufactured with beads and treated with 1 g of biscopearl	92	91	84	84
A: C: D = 1: 1: 1		96	94	89	84
B: C: D = 1: 1: 1		96	92	90	90
A: B: D = 3: 1: 1		92	92	88	81
A: C: D = 3: 1: 1		89	81	77	73
B: C: D = 3: 1: 1		98	98	91	88
A: B: D = 1: 3: 1		89	84	84	78
A: C: D = 1: 3: 1		91	84	84	81
B: C: D = 1: 3: 1		100	94	91	90
A: B: D = 1: 1: 3		94	92	87	82
A: C: D = 1: 1: 3		98	98	94	91
B: C: D = 1: 1: 3		100	96	96	94

A = eucalyptus citridora

B = cymbopogon citratus

C = Xantoxilum Armatum

D = vanillin

As can be seen in Table 3 above, the composition prepared in the composition ratio disclosed in Table 3 was directly treated to investigate the mosquito inflow inhibition into the treatment zone, and even after 120 minutes, eucalyptus citridora: xanthoxylum armatum: vanillin All compositions were able to block more than 80% of mosquito infiltration, except when the composition was mixed at a 3: 1: 1 ratio.

In addition, even when treated with 1 g of the bead agent prepared by supporting the composition prepared in the composition ratios shown in Table 3 in biscopearl (size: 3.8-4.2 mm, Rengo, Tokyo, Japan), almost all the bead agents were at least 80% mosquitoes. The effect of retarding penetration was shown. Therefore, when the active ingredient of the composition according to the present invention is distributed in a space above a certain concentration, it was found that the mosquito is recognized, avoiding the penetration into the space where the repellent material is oriented.

All. Chamber test

Chamber tests were performed to determine the repellency of the compositions of the present invention in large spaces. The chamber used for the test (1.2 X 1.2 X 1.2 m) was manufactured by covering the steel frame with an acrylic plate and installing a square hole (20 X 20 cm) at the bottom of the front side. The square holes were closed with a black card plate with ten holes (each hole 1.5 X 1.5 cm). A mosquito spinning cage (25 × 25 × 25 cm) was connected to the square hole. Another collection cage was placed in the chamber. The spinning cage and the collecting cage were connected with card boards and all wrapped with polyester nets. The fan (model name: EFB-2-3, Hanil, Korea) was operated to promote the diffusion of the components. As a mosquito ape, one human volunteer entered the chamber. With no repellent and only mosquito apes, 2 Count the number of mosquitoes entering the chamber over time. As a repellent, a module containing the beads described below was used. Attractant treatment was performed 30 minutes before the test and repellent treatment was performed 1 hour before the test. Repeated tests were performed to obtain results through statistical treatment.

Beading agents are the same as those used in the cage assay. The composition supported on the bead is a composition (using ethanol as a solvent) in which a ratio of symbobogon citratus: xanthoxilum armatum: vanillin is 8.5: 8.5: 3 (v / v / w).

On the other hand, the module of the shape described in Figure 2 was prepared for this experiment. Make 10 holes to control the opening and closing of the module, each containing the same amount of beading agent, so that a total of about 10 to 11g of beading agent in one module, and approximately 4.7 inside the beading agent To 5.4 g of the composition is supported.

Two sets of ten fully open modules were placed in the chamber described above, and 120 Egyptian forest female mosquitoes were used 5 to 10 days old. The number of female mosquitoes entering the chamber was counted at 5, 10, 20 and 30 minutes after fan operation. Repelling power was calculated by counting the number of incoming mosquitoes (control) and the number of incoming mosquitoes during treatment of the composition of the present invention when no treatment was performed for each time, and calculated the repelling rate by the ratio, and averaged the obtained results as the final repelling rate Confirmed. The unit is percent.

The evasion rate with time was repeated three times to obtain an average evasion rate. The results of the obtained repellent force are shown in Table 4.

The results of the obtained repellent force are shown in Table 4.

Table 4

Evasion force (mean ± SE)
No.	5 minutes	10 minutes	20 minutes
One	66.32 ± 11.35	77.73 ± 8.89	67.81 ± 12.83
2	100 ± 2.1	81.94 ± 1.13	80.56 ± 13.71
3	76.36 ± 8.18	64.22 ± 5.87	64.41 ± 4.50
4	80.56 ± 10.02	55.99 ± 13.04	62.15 ± 8.22

1.Symbopogon Narders: Xantoxilum Armatum: Vanillin = 8.5: 8.5: 3 (v / v / w)

2. Combopogon citratus: Xantoxilum Armatum: Vanillin = 8.5: 8.5: 3 (v / v / w)

3. Simbopogon Narders: Xantoxilum Armatum: Vanillin = 1: 1: 1 (v / v / w)

4. Combopogon citratus: Xantoxilum Armatum: Vanillin = 1: 1: 1 (v / v / w)

From the above Table 4, the composition of the mosquito repellent according to the present invention is excellent even in a large space, in particular the cymbopogon citratus: xanthoxylum armatum: vanillin = 8.5: 8.5: 3 (v / v / w) It can be confirmed that the repellent is excellent.

Meanwhile, in order to check the evasion rate and the service life of the composition of Cymbopogon citratus: Xantoxilum Armatum: vanillin = 8.5: 8.5: 3 (v / v / w), the change of the evasion force over time was examined. The chamber test was conducted and observed. The results obtained are shown in Table 5 below.

Table 5

Iterations	Post Processing Minutes
Iterations	10 minutes	30 minutes	60 minutes
One	100	93.3	93.3
2	87.5	72.7	-
3	100	66.7	70
	Evasion (%, mean ± SE)
Average	95.8 ± 4.2	77.6 ± 8	81.7 ± 11.7

As can be seen in Table 5, the overall average shows a avoidance rate of 83% or more, it can be confirmed that having a avoidance rate of 70% or more for at least 60 minutes.

la. Acceleration test

The sustained duration of the repellency of the repellent composition for blood-sucking arthropods according to the present invention was measured by an accelerated test. Vampire repellent compositions for vampire arthropods use on average about 8 hours a day and have 10 holes through which mosquitoes can move on the walls in the chamber used in the chamber test, but the test is continued for 24 hours continuously for the accelerated test. Inner wall was made into 40 holes. In order to know if the persistence of the repellent composition for blood-absorbing arthropods according to the present invention lasts for 90 days, the effect of the repellency over time after the operation for 8 days under the above conditions was measured.

The test was conducted using the same chamber as the above chamber test except that the holes were increased to 40, and the control group was treated only with ethanol solvent, and the experimental group was simbopogon citratus: xanthoxylum armatum: vanillin = 8.5: 8.5 The composition (solvent is ethanol) of: 3 (v / v / w) was treated. The results obtained are shown in Table 6 below.

Table 6

inflow	Early	10 minutes	40 minutes	50 minutes	60 minutes	Average
Control group (mari)	0	12	13	14	12	12.75
Experiment group (mari)	0	5	7	4	4	5
Evasion (%)	0	58.3	46.2	71.4	66.7	60.8

From Table 6, when the bead treatment carrying the composition according to the present invention, it was confirmed that the average repellent effect of 60% or more lasts more than 90 days.

Claims

Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Xanthoxylum armatum) and crooked A repellent composition for blood-sucking arthropods comprising one plant essential oil and vanillin selected from the group consisting of Cymbopogon nardus .
The method of claim 1,

The repellent composition for blood-absorbing arthropods whose composition of plant essential oils and vanillin is a ratio (v / w) of (1-3): (1-3).
Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Xanthoxylum armatum) and crooked A repellent composition for blood-sucking arthropods comprising two plant essential oils selected from the group consisting of Cymbopogon nardus and vanillin.
The method of claim 3, wherein

Plant essential oil: Another plant essential oil: A blood repellent arthropod repellent composition in which the composition of vanillin is (1 to 3): (1 to 3): (1 to 3) (v / v / w).
The method of claim 4, wherein

Plant Essential Oil: Another Plant Essential Oil: The composition of vanillin is 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3 (v / v / w) Phospholipid repellent arthropod repellent composition.
The method of claim 3, wherein

A repellent composition for vampire arthropods comprising xanthoxylum armatum , cimbopogon citratus and vanillin.
The method of claim 3, wherein

A repellent composition for vampire arthropods comprising xanthoxylum armatum , cymbopogon nardus and vanillin.
A porous carrier comprising the repellent composition for a blood-sucking arthropod according to any one of claims 1 to 7.
Repellent generating device comprising the repellent composition for blood-sucking arthropod according to any one of claims 1 to 7.