WO2012093878A2 - Repellent composition for hematophagous arthropods - Google Patents

Repellent composition for hematophagous arthropods Download PDF

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Publication number
WO2012093878A2
WO2012093878A2 PCT/KR2012/000133 KR2012000133W WO2012093878A2 WO 2012093878 A2 WO2012093878 A2 WO 2012093878A2 KR 2012000133 W KR2012000133 W KR 2012000133W WO 2012093878 A2 WO2012093878 A2 WO 2012093878A2
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Prior art keywords
vanillin
repellent
composition
repellent composition
blood
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PCT/KR2012/000133
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French (fr)
Korean (ko)
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WO2012093878A4 (en
WO2012093878A3 (en
Inventor
이성화
안용준
권형욱
박형호
김순일
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엘지전자 주식회사
서울대학교 산학협력단
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Priority claimed from KR1020120001336A external-priority patent/KR101430492B1/en
Application filed by 엘지전자 주식회사, 서울대학교 산학협력단 filed Critical 엘지전자 주식회사
Priority to RU2013132015/10A priority Critical patent/RU2542485C1/en
Priority to BR112013017173A priority patent/BR112013017173A2/en
Publication of WO2012093878A2 publication Critical patent/WO2012093878A2/en
Publication of WO2012093878A3 publication Critical patent/WO2012093878A3/en
Publication of WO2012093878A4 publication Critical patent/WO2012093878A4/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/04Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents

Definitions

  • the present invention relates to a repellent composition for blood-sucking arthropods, and more particularly, to a blood-sucking arthropod that improves repelling power and increases the duration of repelling power for blood-sucking arthropods including plant essential oils and vanillin.
  • a repellent composition for animals is a repellent composition for animals.
  • Vampire arthropods vary from mosquitoes to ticks, stable fly, tsetsefly, horse fly, and bed bugs.
  • mosquitoes which are the most important blood-sucking arthropods, are the major mediators of malaria, including dengue fever, yellow fever, encephalitis and West Nile virus, affecting more than 3-5 billion people a year. The death toll alone causes serious harm of more than 1 million people per year (Rozendaal, JA 1997.
  • Vector control methods for use by individuals and communities.WHO.Geneva, Switzerland.:http://www.who.int/water_sanitation_health / resources / vectorcontrol / en /).
  • DEET N, N-diethyl-3-methylbenzamide
  • DEET N, N-diethyl-3-methylbenzamide
  • TM central nervous system suppression
  • dermatitis and mammalian DNA damage.
  • Insect repellents historical perspectives and new developments. J. Am. Acad. Dermatol. 58: 865-871 .; Knowles, CO 1991. Miscellaneous pesticides, pp. 1471- 1526. In JB Hayes, Jr. and ER Laws, Jr. [eds.], Handbook of pesticide toxicology.Academic Press, San Diego, CA.
  • Plant essential oils are useful because plants contain a variety of biologically active substances and have relatively little effect on the environment and organisms and are also relatively inexpensive compared to plant extracts.
  • an object of the present invention is to solve the problems of the conventional synthetic repellent, to provide a safer and more repellent for vampire arthropods and to provide a repellent composition for vampire arthropods to increase the duration of the repellent force.
  • the repellent composition for blood-sucking arthropods is characterized in that it comprises plant essential oils and vanillin.
  • a porous carrier comprising a repellent composition for blood-absorbing arthropods according to the present invention.
  • the repellent composition for blood-sucking arthropod according to the present invention and the carrier and repellent generating device including the same improve the repelling force and increase the duration of the blood-sucking arthropod, and are safer than conventional repellents.
  • the figure which shows the test cage used for the cage test method which concerns on this invention.
  • the cage is framed by an iron frame, the material handling zones 1 and the spinning cage 3 located at both ends are wrapped in polyester nets, and the center untreated zone 2 is wrapped with acrylic plates.
  • the holes in the polyester net are examples only and are not proportional to the figure and are small enough to prevent mosquitoes from escaping.
  • FIG. 2 is an illustration of a module comprising a bead made according to the present invention.
  • the repellent composition for blood-sucking arthropods according to the present invention includes plant essential oils and vanillin.
  • the blood-sucking arthropod to which the repellent composition of the present invention can be applied refers to all living organisms that take blood or body fluids from the skin of mammals or birds, for example, vampires of mosquitoes, insects, chimneys, fly flies, and the like. Flies, baboons, stools, and the like.
  • the plant essential oils used in the present invention can be used without particular limitation as long as they are known as repellents for vampire arthropods.
  • it cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Zanthoxylum armatum ) And cymbopogon nardus .
  • the plant essential oil of the present invention is obtained from the above-mentioned plants, and is cooled by a conventional solvent (eg, water, ethanol, acetone, methanol, propanol, ether, hexane, ethyl acetate, chloroform or a mixed solvent thereof), warm It may be obtained by using one of methods such as immersion method, hot water extraction method (electronic method) and fermentation extraction method, filtration leaching method, supercritical fluid extraction method, and more preferably may be obtained by steam distillation method. In addition, it may be obtained from each plant part, for example, roots, stems, leaves, pulp, flowers, fruits, seeds, and / or bark, or the whole plant.
  • a conventional solvent eg, water, ethanol, acetone, methanol, propanol, ether, hexane, ethyl acetate, chloroform or a mixed solvent thereof
  • a conventional solvent eg, water, ethanol, acetone, methanol,
  • Vanillin used in the present invention is 4-hydroxy-3-methoxy benzaldehyde as one of the vanilla-smelling aromatic aldehydes. There are no particular restrictions on the form or method of use, and commercially available products can be purchased and used. Meanwhile, various substances have been known in relation to blood repellent arthropods, but vanillin is not known as an insect repellent.
  • the repellent composition for blood-sucking arthropods is Cinnamomum cassia , Rosmarinus officinalis , Eucalyptus citriodora , Cimbopogon citratus ), Zanthoxylum armatum and Cymbopogon nardus , one of the plant essential oils selected from the group consisting of vanillin.
  • the composition ratio of plant essential oil and vanillin is not particularly limited, but a weight ratio of (1 to 3) to (1 to 3) is preferable in view of solubility in solvents and mutual mixing.
  • the repellent composition for blood-sucking arthropods according to the present invention may include two kinds of plant essential oils and vanillin.
  • the composition ratio of each plant essential oil and vanillin is not particularly limited, but the ratio of plant essential oil: another type of plant essential oil: vanillin in consideration of maximization of solubility in solvents, mutual mixing, and avoidance is (1 to 3). It is preferable to set it as ratio (v / v / w) of (1-3): (1-3). More preferably, it is the ratio (v / v / w) of 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3.
  • the blood-absorbing arthropod repellent composition according to the present invention may be formulated further comprising suitable additives, excipients or carriers depending on the intended use or application environment.
  • suitable additives include emulsions, emulsifiers, hydrating agents, water soluble agents, aerosols, pump sprays, suspensions, ULV formulations, fragrances, granules, powders, powders, granules, capsules, ointments, creams, It may be formulated into a bead, tablet, extract, fluid extract, lotion, met, and the like. It is preferably applied as cream, pump spray or aerosol.
  • the repellent composition for blood-absorbing arthropods according to the present invention can be applied to a porous carrier for sustained release.
  • the porous carrier used at this time is not particularly limited, for example charcoal (silco gel), silica gel (silica gel), diatomaceous earth (diatomearth) and the like.
  • the porous carrier is a gel (gel) formed to include a micropore as a bond between the units constituting the polymer, or biscopearl (viscopearl, Rengo, Tokyo, Japan), etc. produced by compressing the polymer or polymer fiber It includes. Since the biscopearl provides excellent porosity, it is suitable for the gradual release of the repellent composition for vampire arthropods according to the present invention.
  • the route of application of the repellent composition for blood-sucking arthropods according to the present invention is not limited thereto, but the dispersant composition may be directly dispersed or dispersed in areas where blood-sucking arthropods occur, or mammals including humans or domestic animals or It can be used directly on the skin of birds, or can be applied to household items such as clothes, bags, bands, tents, and indoors.
  • vampire arthropods In areas where vampire arthropods are predominantly occurring, they can be distributed or dispersed directly in the air. Or, for example, when applied to a certain space, such as in a building, it may be subjected to a porous carrier that continuously releases the repellent composition.
  • a capsule carrying the repellent composition when applied to household goods, a capsule carrying the repellent composition is prepared, and the repellent composition carried in the air when the capsule is bursted by friction by manufacturing the cloth, cloth, and band, which is a material of the household goods, or directly incorporating into plastics. It can be used by being diverted to show repelling power or being directly mixed and then slowly released into the air.
  • the repellent composition according to the present invention may be supported on a porous carrier.
  • an indicator is included in the device for gradually releasing the repellent composition, so that when the repellent composition, which is an active substance, is all volatilized, for example, the service life may be displayed by discoloration.
  • the blood repellent arthropod repellent composition according to the present invention can also be applied to household appliances such as an air purifier, an air conditioner, and an air conditioner.
  • household electrical appliances can be appropriately modified in accordance with conventional methods.
  • Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), crooked pogon Carry Traders (Cimbopogon nardus) and vanillin was purchased from Berje, Bloomfield, NJ, and Zanthoxylum armatum was purchased from Seema International, Deli, India. And DEET were purchased from Sigma-Aldrich (St. Louis, Mo.).
  • plant essential oil and vanillin were dissolved in ethanol to prepare a composition.
  • Plant essential oil and vanillin were respectively mixed at a ratio of 5%: 5% (v / w) and 5%: 15% (v / w), and dissolved in ethanol to obtain a final 100 ⁇ l of the composition, respectively.
  • a composition comprising two plant essential oils and vanillin
  • two different plants from eucalyptus citridora, cymbopogon citratus, and xanthoxylum armatum are selected from plant essential oils in ethanol together with vanillin.
  • Each 100 ⁇ l of the composition was prepared by melting.
  • the mixing ratios of the two plant essential oils and vanillin were 1: 1: 1, 3: 1: 1, 1: 3: 1 and 1: 1: 3, respectively, which were 5%: 5%: 5% and 15%: 5%. Same as the 5%, 5%: 15%: 5%, and 5%: 5%: 15% (v / v / w) ratios.
  • cymbopogon Narders and Xantoxilum Armatum and Vanillin were prepared in a ratio of 8.5: 8.5: 3
  • Eucalyptus citridora, Xantoxilum Armatum and Vanillin were prepared in a ratio of 8.5: 8.5: 3 (v / v / w).
  • the skin test was carried out by uniformly treating the skin surface of the tester exposed to the back of the rubber glove hand with a hole of 5 cm diameter in the preparation of the material.
  • the test subjects were healthy 21-40 year old men.
  • the control group treated only 100 ⁇ l of ethanol.
  • the complete avoidance duration was defined as the time between treatment and the time of initial vampires.
  • Tables 1-2 show the complete avoidance duration (CPT) for Egyptian mosquitoes adults of plant essential oils and vanillin and a composition of one plant essential oil and vanillin. As a control, complete evacuation duration (CPT) is described together for Egyptian adult mosquitoes in DEET alone.
  • Table 2 shows the results for the complete avoidance duration (CPT) for the adult Egyptian mosquitoes in the composition of two plant essential oils and vanillin.
  • Cinnamomum cassia 5% alone treatment showed a 75-minute CPT, cymbopogon citratus and xantoxilum armatum 30 minutes and eucalyptus citridora and vanillin 22.5 minutes and At 15 minutes, Rosemarinus Offinalis had no effect.
  • DEET showed the strongest evasion at 127.5 minutes and improved to 247.5 minutes at 15%.
  • the composition containing the plant essential oil and vanillin it was found that the repelling power is improved while having a synergistic effect compared to when using each alone.
  • A is a group of eucalyptus Rio Dora Tooth sheet: pogon Citra crooked tooth: vanillin
  • Group B is Eucalyptus Citridora: Xantoxilum Armatum: Vanillin
  • Group C is Cymbopogon Cilatus: Xantoxilum Armatum: Vanillin
  • Group D is Simbopogon Narders: Xantoxilum Armatum: Vanillin
  • This test is to test whether mosquitoes invade the treatment area through a thick card board (building window) that is laid horizontally in the center of the test system and has a 10 cm hole in the center. In addition, this test is to assay the average stopping power over time of the bead formulation.
  • compositions according to the invention were treated with 5 ⁇ l as needed or prepared beads.
  • the bead was prepared by supporting the repellent composition according to the present invention in a biscopearl (3.8-4.2 mm, Rengo, Tokyo, Japan), which is a porous cellulose bead.
  • the composition was about 0.465 g, and the weight of the biscopearl was about 0.535 g.
  • the ratio between each component of the composition used is summarized in the following table.
  • the test cage is illustrated in FIG. 1.
  • the cage is made of a steel frame to produce a material treatment zone (1), an untreated zone (2) and a spinning cage (3), the material handling zone (1) and a spinning cage (3) are wrapped in a polyester net, and the untreated zone (2) put an acrylic plate.
  • a thick card board having a hole of 10 cm in diameter was installed in the center of the non-treatment zone 2. 10 minutes before the start of the test so that one mouse as a mosquito ape with a mosquito ape is immobilized in the material processing zone 1, and the room for treating the repellent composition according to the present invention and the bead agent are placed in the material processing zone (1).
  • the composition was allowed to dry naturally for 1 minute and suspended by thread on top of the material treatment zone 1 and allowed to volatilize for 3 minutes. Thereafter, female adult female mosquitoes in the spinning cage 3 were spun. The natural flow of mosquitoes in this test is the material handling zone (1) from the mosquito spinning cage (3).
  • the average rate of inhibition for preventing the ingress of mosquitoes into the material treatment zone 1 was calculated using the following formula.
  • % Blockage ⁇ (Mosquitoes penetrated into 30-treatment zone) / 30 ⁇ ⁇ 100
  • A eucalyptus citridora
  • compositions prepared in the composition ratio disclosed in Table 3 were directly treated to investigate the mosquito inflow inhibition into the treatment zone, and even after 120 minutes, eucalyptus citridora: xanthoxylum armatum: vanillin All compositions were able to block more than 80% of mosquito infiltration, except when the composition was mixed at a 3: 1: 1 ratio.
  • Chamber tests were performed to determine the repellency of the compositions of the present invention in large spaces.
  • the chamber used for the test (1.2 X 1.2 X 1.2 m) was manufactured by covering the steel frame with an acrylic plate and installing a square hole (20 X 20 cm) at the bottom of the front side. The square holes were closed with a black card plate with ten holes (each hole 1.5 X 1.5 cm).
  • a mosquito spinning cage (25 ⁇ 25 ⁇ 25 cm) was connected to the square hole.
  • Another collection cage was placed in the chamber. The spinning cage and the collecting cage were connected with card boards and all wrapped with polyester nets.
  • the fan (model name: EFB-2-3, Hanil, Korea) was operated to promote the diffusion of the components. As a mosquito ape, one human volunteer entered the chamber.
  • Beading agents are the same as those used in the cage assay.
  • the composition supported on the bead is a composition (using ethanol as a solvent) in which a ratio of symbobogon citratus: xanthoxilum armatum: vanillin is 8.5: 8.5: 3 (v / v / w).
  • the module of the shape described in Figure 2 was prepared for this experiment. Make 10 holes to control the opening and closing of the module, each containing the same amount of beading agent, so that a total of about 10 to 11g of beading agent in one module, and approximately 4.7 inside the beading agent To 5.4 g of the composition is supported.
  • the overall average shows a avoidance rate of 83% or more, it can be confirmed that having a avoidance rate of 70% or more for at least 60 minutes.
  • the sustained duration of the repellency of the repellent composition for blood-sucking arthropods according to the present invention was measured by an accelerated test.
  • Vampire repellent compositions for vampire arthropods use on average about 8 hours a day and have 10 holes through which mosquitoes can move on the walls in the chamber used in the chamber test, but the test is continued for 24 hours continuously for the accelerated test. Inner wall was made into 40 holes.
  • the persistence of the repellent composition for blood-absorbing arthropods according to the present invention lasts for 90 days, the effect of the repellency over time after the operation for 8 days under the above conditions was measured.

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Abstract

The present invention relates to a repellent composition for hematophagous arthropods. The repellent composition comprises vanilline and one or two kinds of essential oils selected from the group consisting of Cinnamomum cassia, Rosmarinus officinalis, Eucalyptus citriodora, Cimbopogon citratus, Zanthoxylum armatum, and Cimbopogon nardus. Therefore, the repellency for hematophagous arthropods is improved and duration time of the repellency is increased. Additionally, the invention relates to a porous carrier containing the repellent composition for hematophagous arthropods, and a repellent-generating apparatus.

Description

흡혈성 절지 동물용 기피제 조성물Blood repellent arthropod repellent composition
본 발명은 흡혈성 절지 동물용 기피제 조성물에 관한 것으로, 보다 구체적으로는 식물 정유 및 바닐린을 포함하여 모기 등의 흡혈성 절지 동물에 대한 기피력을 향상시키고 기피력의 지속시간을 증가시키는 흡혈성 절지 동물용 기피제 조성물에 관한 것이다. The present invention relates to a repellent composition for blood-sucking arthropods, and more particularly, to a blood-sucking arthropod that improves repelling power and increases the duration of repelling power for blood-sucking arthropods including plant essential oils and vanillin. A repellent composition for animals.
흡혈성 절지 동물들은 모기(mosquito)를 비롯해 진드기(tick), 파리(stable fly, tsetsefly), 등에(horse fly), 빈대(bed bug) 등 매우 다양하다. 특히, 흡혈성 절지 동물로서 가장 중요한 위치를 점하고 있는 모기는 뎅기열, 황열병, 뇌염, 웨스트나일바이러스를 비롯한 말라리아의 주 매개자로서 년간 3-5억 명 이상에게 영향을 미치고 있고, 이 중 말라리아로 인한 사망자만도 매년 1백만명 이상에 달할 정도로 심각한 위해를 끼치고 있다(Rozendaal, J. A. 1997. Vector control: methods for use by individuals and communities. WHO. Geneva, Switzerland.:http://www.who.int/water_sanitation_health/resources/vectorcontrol/en/).Vampire arthropods vary from mosquitoes to ticks, stable fly, tsetsefly, horse fly, and bed bugs. In particular, mosquitoes, which are the most important blood-sucking arthropods, are the major mediators of malaria, including dengue fever, yellow fever, encephalitis and West Nile virus, affecting more than 3-5 billion people a year. The death toll alone causes serious harm of more than 1 million people per year (Rozendaal, JA 1997. Vector control: methods for use by individuals and communities.WHO.Geneva, Switzerland.:http://www.who.int/water_sanitation_health / resources / vectorcontrol / en /).
그리하여 흡혈성 절지동물들에 대한 다양한 기피제가 개발되어 있다. 이러한 기피제는 사람과 가축들을 생활방해 해충들에 의한 흡혈로부터 예방해 주는 가장 효과적인 수단으로 알려져 있다(Curtis, C. F., J. D. Lives, L. U. Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75-92. In C.F. Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL.; Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and increasingly regulated world. Annu. Rev. Entomol. 51: 45-66 참조). Thus, various repellents have been developed for vampire arthropods. Such repellents are known to be the most effective means of preventing humans and livestock from vampires by harmful pests (Curtis, CF, JD Lives, LU Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75- 92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and increasingly regulated world. Annu. Rev. Entomol. 51: 45-66).
그러나 가장 널리 사용되고 있는 기피제인 DEET(N,N-diethyl-3-methylbenzamide)는 그 효과에도 불구하고 불쾌한 냄새, 플라스틱이나 고무 손상, 중추신경계 억제, 피부염 유발, 포유동물 DNA 손상 등 다양한 부작용들이 알려져 있다(Katz, T. M., J. H. Miller, and A. A. Hebert. 2008. Insect repellents: historical perspectives and new developments. J. Am. Acad. Dermatol. 58: 865-871.; Knowles, C. O. 1991. Miscellaneous pesticides, pp. 1471-1526. In J. B. Hayes, Jr. and E. R. Laws, Jr. [eds.], Handbook of pesticide toxicology. Academic Press, San Diego, CA. 참조). 그리하여 흡혈 생물들로부터 예방이 가능한 보다 안전한 형태의 기피제들이 필요한 실정이다.However, DEET (N, N-diethyl-3-methylbenzamide), which is the most widely used repellent, is known to have various side effects such as unpleasant odor, plastic or rubber damage, central nervous system suppression, dermatitis, and mammalian DNA damage. (Katz, TM, JH Miller, and AA Hebert. 2008. Insect repellents: historical perspectives and new developments. J. Am. Acad. Dermatol. 58: 865-871 .; Knowles, CO 1991. Miscellaneous pesticides, pp. 1471- 1526. In JB Hayes, Jr. and ER Laws, Jr. [eds.], Handbook of pesticide toxicology.Academic Press, San Diego, CA. Thus, there is a need for safer forms of repellent that can be prevented from vampires.
한편, 최근에는 흡혈성 절지 동물들에 대한 천연 기피제를 개발할 수 있는 대체 자원으로서 식물 정유가 관심을 받고 있다(Ahn, Y. J., S. I. Kim, H. K. Kim, and J. H. Tak. 2006. Naturally occurring house dust mites control agents: development and commercialization, pp. 269-289. In M. Rai and M. C. Carpinella [eds.], Naturally occurring bioactive compounds. Elsevier, London, United Kingdom.; Curtis, C. F., J. D. Lives, L. U. Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75-92. In C.F. Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL.; Das, N. G., I. Baruah, P. K. Talukdar, and S. C. Das. 2003. Evaluation of botanicals as repellents against mosquitoes. J. Vector Borne Dis. 40: 49-53.; Rozendaal, J. A. 1997. Vector control: methods for use by individuals and communities. World Health Organization, Geneva, Switzerland 참조). 식물 정유는 식물체가 다양한 생물 활성 물질들을 함유하고 있고 환경 및 생물들에게 비교적 별다른 영향을 미치지 않으며 또한 식물 추출물들에 비해 상대적으로 값이 싸기에 유용하다. Recently, plant essential oils have attracted attention as an alternative resource for developing natural repellents for vampire arthropods (Ahn, YJ, SI Kim, HK Kim, and JH Tak. 2006. Naturally occurring house dust mites control agents: development and commercialization, pp. 269-289.In M. Rai and MC Carpinella [eds.], Naturally occurring bioactive compounds.Elsevier, London, United Kingdom .; Curtis, CF, JD Lives, LU Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75-92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Das, N. G., I. Baruah, P. K. Talukdar, and S. C. Das. 2003. Evaluation of botanicals as repellents against mosquitoes. J. Vector Borne Dis. 40: 49-53; Rozendaal, J. A. 1997. Vector control: methods for use by individuals and communities. World Health Organization, Geneva, Switzerland). Plant essential oils are useful because plants contain a variety of biologically active substances and have relatively little effect on the environment and organisms and are also relatively inexpensive compared to plant extracts.
이에 본 발명의 목적은 기존의 합성 기피제의 문제점을 해결하고 보다 안전하며 흡혈성 절지 동물에 대한 기피력을 향상시키고 기피력의 지속시간을 증가시키는 흡혈성 절지 동물용 기피제 조성물을 제공하려는 것이다. Therefore, an object of the present invention is to solve the problems of the conventional synthetic repellent, to provide a safer and more repellent for vampire arthropods and to provide a repellent composition for vampire arthropods to increase the duration of the repellent force.
본 발명의 또 다른 목적은 이러한 기피제 조성물을 적용한 담체 및 발생 장치를 제공하려는 것이다. It is another object of the present invention to provide a carrier and a generating device to which such a repellent composition is applied.
상기 목적을 달성하기 위하여, 본 발명에 따른 흡혈성 절지 동물용 기피제 조성물은 식물 정유 및 바닐린을 포함하는 것을 특징으로 한다. In order to achieve the above object, the repellent composition for blood-sucking arthropods according to the present invention is characterized in that it comprises plant essential oils and vanillin.
또 다른 목적을 달성하기 위하여, 본 발명에 따른 흡혈성 절지 동물용 기피제 조성물을 포함하는 다공성 담체를 제공한다. In order to achieve another object, it provides a porous carrier comprising a repellent composition for blood-absorbing arthropods according to the present invention.
또한, 또 다른 목적을 달성하기 위하여, 본 발명에 따른 흡혈성 절지 동물용 기피제 발생 장치를 포함하는 것을 특징으로 한다. In addition, in order to achieve another object, it characterized in that it comprises a repellent generating device for blood-sucking arthropod according to the present invention.
본 발명에 따른 흡혈성 절지 동물용 기피제 조성물 및 이를 포함하는 담체 및 기피제 발생 장치는 흡혈성 절지 동물에 대한 기피력을 향상시키고 그 지속시간을 증가시킬 뿐만 아니라 기존의 기피제에 비해 더욱 안전하다.The repellent composition for blood-sucking arthropod according to the present invention and the carrier and repellent generating device including the same improve the repelling force and increase the duration of the blood-sucking arthropod, and are safer than conventional repellents.
도 1은 본 발명에 따른 케이지 시험법에 사용하는 시험용 케이지를 나타내는 그림이다. 상기 케이지는 철제 프레임으로 틀을 형성하고, 양 끝에 위치한 물질 처리 구역(1)과 방사 케이지(3)는 폴리에스터 네트로 감싸고, 가운데에 위치한 무처리 구역(2)은 아크릴 판으로 감싼다. 폴리에스터 네트의 구멍은 예시일 뿐 그림에 비례하는 것이 아니며, 모기가 빠져 나갈 수 없을 만큼 충분히 작다. BRIEF DESCRIPTION OF THE DRAWINGS The figure which shows the test cage used for the cage test method which concerns on this invention. The cage is framed by an iron frame, the material handling zones 1 and the spinning cage 3 located at both ends are wrapped in polyester nets, and the center untreated zone 2 is wrapped with acrylic plates. The holes in the polyester net are examples only and are not proportional to the figure and are small enough to prevent mosquitoes from escaping.
도 2는 본 발명에 따라 제조된 비드제를 포함하는 모듈의 예시이다. 2 is an illustration of a module comprising a bead made according to the present invention.
본 발명에 따른 흡혈성 절지 동물용 기피제 조성물은 식물 정유 및 바닐린을 포함한다. The repellent composition for blood-sucking arthropods according to the present invention includes plant essential oils and vanillin.
본 발명의 기피제 조성물이 적용될 수 있는 흡혈성 절지 동물은 포유류 또는 조류의 피부로부터 혈액 또는 체액을 취하여 살아가는 모든 생물들을 의미하는 것으로, 예를 들면 모기과 곤충들 및 침파리, 체체파리, 등에 등의 흡혈 파리류, 빈태, 사면발이, 이 등이다. The blood-sucking arthropod to which the repellent composition of the present invention can be applied refers to all living organisms that take blood or body fluids from the skin of mammals or birds, for example, vampires of mosquitoes, insects, chimneys, fly flies, and the like. Flies, baboons, stools, and the like.
본 발명에 사용되는 식물 정유는 흡혈성 절지동물에 대한 기피제로서 알려져 있는 것이라면 특별한 제한 없이 사용될 수 있다. 바람직하게는 신나모멈 카시아(Cinnamomum cassia), 로즈마리너스 오피시날리스(Rosmarinus officinalis), 유칼리투스 시트리오도라(Eucalyptus citriodora), 심보포곤 시트라투스(Cimbopogon citratus), 잔톡실럼 아르마툼(Zanthoxylum armatum), 심보포곤 나르더스 (Cymbopogon nardus) 등에서 유래한 것이다. 본 발명의 식물 정유는 상기 식물체들로부터 수득되는 것으로서, 통상의 용매(예컨대, 물, 에탄올, 아세톤, 메탄올, 프로판올, 에테르, 헥산, 에틸아세테이트, 클로로포름 또는 이들의 혼합 용매)를 이용한 냉침법, 온침법, 열수추출법(전자법) 그리고 발효추출법, 여과침출법, 초임계유체추출법 등의 방법들 중 하나를 활용해서 얻어지는 것일 수 있고 더욱 바람직하게는 수증기 증류법으로 수득한 것일 수 있다. 또한, 식물 부위별, 예컨대, 뿌리, 줄기, 잎, 과육, 꽃, 열매, 종자, 및/또는 껍질 등으로부터 각각 얻거나 식물 전체로부터 얻은 것일 수 있다.The plant essential oils used in the present invention can be used without particular limitation as long as they are known as repellents for vampire arthropods. Preferably it cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Zanthoxylum armatum ) And cymbopogon nardus . The plant essential oil of the present invention is obtained from the above-mentioned plants, and is cooled by a conventional solvent (eg, water, ethanol, acetone, methanol, propanol, ether, hexane, ethyl acetate, chloroform or a mixed solvent thereof), warm It may be obtained by using one of methods such as immersion method, hot water extraction method (electronic method) and fermentation extraction method, filtration leaching method, supercritical fluid extraction method, and more preferably may be obtained by steam distillation method. In addition, it may be obtained from each plant part, for example, roots, stems, leaves, pulp, flowers, fruits, seeds, and / or bark, or the whole plant.
본 발명에 사용되는 바닐린은 바닐라향이 나는 방향족 알데하이드의 하나로 4-하이드록시-3-메톡시 벤즈알데하이드이다. 그 형태나 사용방법에 특별한 제한이 없으며 시중에서 시판되고 있는 것을 구입해서 사용할 수 있다. 한편, 흡혈성 절지 동물용 기피제에 관련하여 여러가지 물질들이 공지된 바 있으나, 바닐린은 곤충 기피제로 알려진 바 없다. Vanillin used in the present invention is 4-hydroxy-3-methoxy benzaldehyde as one of the vanilla-smelling aromatic aldehydes. There are no particular restrictions on the form or method of use, and commercially available products can be purchased and used. Meanwhile, various substances have been known in relation to blood repellent arthropods, but vanillin is not known as an insect repellent.
본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물은 신나모멈 카시아(Cinnamomum cassia), 로즈마리너스 오피시날리스(Rosmarinus officinalis), 유칼리투스 시트리오도라(Eucalyptus citriodora), 심보포곤 시트라투스(Cimbopogon citratus), 잔톡실럼 아르마툼(Zanthoxylum armatum) 및 심보포곤 나르더스(Cymbopogon nardus)로 이루어진 그룹으로부터 선택되는 식물 정유 중 1종 및 바닐린을 포함하는 것을 특징으로 한다. 이때 식물 정유와 바닐린의 조성 비율은 특별한 제한이 없으나 (1 내지 3) : (1 내지 3)의 중량비율로 하는 것이 용매에의 용해성 및 상호 혼합성 면에서 바람직하다.The repellent composition for blood-sucking arthropods according to the present invention is Cinnamomum cassia , Rosmarinus officinalis , Eucalyptus citriodora , Cimbopogon citratus ), Zanthoxylum armatum and Cymbopogon nardus , one of the plant essential oils selected from the group consisting of vanillin. At this time, the composition ratio of plant essential oil and vanillin is not particularly limited, but a weight ratio of (1 to 3) to (1 to 3) is preferable in view of solubility in solvents and mutual mixing.
또한 본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물은 상기 식물 정유 2종 및 바닐린을 포함할 수 있다. 이때 각각의 식물 정유 및 바닐린의 조성 비율은 특별한 제한이 없으나 용매에의 용해성, 상호 혼합성 및 기피력의 극대화를 고려하여 식물 정유 : 또 다른 종류의 식물 정유 : 바닐린의 조성 비율은 (1 내지 3) : (1 내지 3) : (1 내지 3)의 비율(v/v/w)로 하는 것이 바람직하다. 더욱 바람직하게는 1:1:1, 3:1:1, 1:3:1, 1:1:3 또는 8.5:8.5:3의 비율(v/v/w)이다. In addition, the repellent composition for blood-sucking arthropods according to the present invention may include two kinds of plant essential oils and vanillin. At this time, the composition ratio of each plant essential oil and vanillin is not particularly limited, but the ratio of plant essential oil: another type of plant essential oil: vanillin in consideration of maximization of solubility in solvents, mutual mixing, and avoidance is (1 to 3). It is preferable to set it as ratio (v / v / w) of (1-3): (1-3). More preferably, it is the ratio (v / v / w) of 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3.
본 발명에 따른 흡혈성 절지 동물용 기피제 조성물은 사용 목적이나 적용 환경에 따라 적절한 첨가제나 부형제 또는 담체를 더 포함하여 제형화될 수 있다. 예를 들면 유제, 유화제, 수화제, 수용제, 에어로졸제, 펌프 스프레이제, 현탁제, ULV(Ultra Low Volume) 제제, 방향수제, 과립제, 분제, 산제, 미립제, 캡슐제, 연고제, 크림제, 비드제, 정제, 엑스제, 유동엑스제, 로션제, 메트 등으로 제형화하여 사용할 수 있다. 바람직하게는 크림제, 펌프 스프레이제 또는 에어로졸제로 적용된다. The blood-absorbing arthropod repellent composition according to the present invention may be formulated further comprising suitable additives, excipients or carriers depending on the intended use or application environment. Examples include emulsions, emulsifiers, hydrating agents, water soluble agents, aerosols, pump sprays, suspensions, ULV formulations, fragrances, granules, powders, powders, granules, capsules, ointments, creams, It may be formulated into a bead, tablet, extract, fluid extract, lotion, met, and the like. It is preferably applied as cream, pump spray or aerosol.
또한 본 발명에 따른 흡혈성 절지 동물용 기피제 조성물은 지속 방출을 위해 다공성 담체에 적용하여 사용할 수 있다. 이때 사용되는 다공성 담체는 특별한 제한은 없으나 예를 들어 차콜(charcoal), 실리카겔(silica gel), 규조토(diatomearth) 등을 들 수 있다. 상기 다공성 담체는 폴리머를 이루는 단위체간의 결합으로 미소구멍(micropore)을 포함하도록 형성되는 겔(gel)이나, 또는 폴리머 또는 고분자 섬유질을 압착하여 생성되는 비스코펄(viscopearl, Rengo 社, Tokyo, Japan) 등을 포함한다. 상기 비스코펄은 탁월한 다공성을 제공하므로, 본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물의 점진적인 방출에 적합하다. In addition, the repellent composition for blood-absorbing arthropods according to the present invention can be applied to a porous carrier for sustained release. The porous carrier used at this time is not particularly limited, for example charcoal (silco gel), silica gel (silica gel), diatomaceous earth (diatomearth) and the like. The porous carrier is a gel (gel) formed to include a micropore as a bond between the units constituting the polymer, or biscopearl (viscopearl, Rengo, Tokyo, Japan), etc. produced by compressing the polymer or polymer fiber It includes. Since the biscopearl provides excellent porosity, it is suitable for the gradual release of the repellent composition for vampire arthropods according to the present invention.
본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물의 적용 경로는, 이에 제한되는 것은 아니지만, 상기 기피제 조성물을 흡혈성 절지 동물들이 주로 발생하는 지역에 직접 분산 또는 산포하거나, 사람 또는 가축을 포함하는 포유류 또는 조류의 피부에 직접 사용하거나, 의류, 가방, 밴드, 텐트 등의 생활용품이나 실내에 적용할 수 있다. The route of application of the repellent composition for blood-sucking arthropods according to the present invention is not limited thereto, but the dispersant composition may be directly dispersed or dispersed in areas where blood-sucking arthropods occur, or mammals including humans or domestic animals or It can be used directly on the skin of birds, or can be applied to household items such as clothes, bags, bands, tents, and indoors.
흡혈성 절지 동물들이 주로 발생하는 지역에 적용시에는 공기 중에 직접 분산시키거나 산포하여 적용할 수 있다. 또는 예를 들어, 건물 내와 같이 일정 공간에 적용하는 경우, 기피제 조성물을 지속적으로 방출하는 다공성 담체를 거치할 수 있다. In areas where vampire arthropods are predominantly occurring, they can be distributed or dispersed directly in the air. Or, for example, when applied to a certain space, such as in a building, it may be subjected to a porous carrier that continuously releases the repellent composition.
포유류나 조류의 피부에 직접 사용하는 경우, 적당량을 피부에 도포 또는 분사하여 사용할 수 있다. 피부에 직접 사용하는 경우, 자극성이 없거나, 있더라도 피부 문제를 일으키지 않을 정도로 극히 적어야 한다. When used directly on the skin of mammals or birds, an appropriate amount may be applied or sprayed on the skin. If used directly on the skin, it should be extremely small, irritating or even if it does not cause skin problems.
또한 생활용품에 적용할 때에는 기피제 조성물을 담지한 캡슐을 제조하여 생활용품의 재료인 옷감이나 천, 밴드 제조시 또는 플라스틱류에 직접 혼입 가공함으로써 캡슐이 마찰에 의해 터질 때 담지된 기피제 조성물이 공기 중으로 발산하여 기피력을 나타내거나 또는 직접 혼입되어 있다가 공기 중으로 서서히 방출되게 하여 사용할 수 있다. 이러한 예로서, 다공성 담체에 본 발명에 따르는 기피제 조성물을 담지하여 사용할 수 있다. 이때, 상기 기피제 조성물을 점진적으로 방출하는 장치에 인디케이터(indicator)를 포함시켜 유효물질인 기피제 조성물이 모두 휘산되면 예를 들어 변색 등으로 사용 수명을 표시되도록 할 수 있다. In addition, when applied to household goods, a capsule carrying the repellent composition is prepared, and the repellent composition carried in the air when the capsule is bursted by friction by manufacturing the cloth, cloth, and band, which is a material of the household goods, or directly incorporating into plastics. It can be used by being diverted to show repelling power or being directly mixed and then slowly released into the air. As such an example, the repellent composition according to the present invention may be supported on a porous carrier. In this case, an indicator (indicator) is included in the device for gradually releasing the repellent composition, so that when the repellent composition, which is an active substance, is all volatilized, for example, the service life may be displayed by discoloration.
본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물은 또한 공기 청정기, 공기 조화기, 에어컨 등의 가전 제품에 적용할 수 있다. 이러한 가전 제품에의 적용은 통상의 방법에 따라 적절히 변형 가능하다. 상술한 가전 제품에의 적용은 공기 청정기, 공기 조화기, 에어컨 등 집안 내부의 공기를 대류시키는 기능을 가진 경우, 정화된 공기에 본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물이 발산되도록 할 수 있으므로 특히 유용하다. The blood repellent arthropod repellent composition according to the present invention can also be applied to household appliances such as an air purifier, an air conditioner, and an air conditioner. Application to such household electrical appliances can be appropriately modified in accordance with conventional methods. Application to the above-mentioned home appliances, such as an air purifier, air conditioner, air conditioner, if it has the function to convection the air inside the house, it is possible to make the repellent composition for vampire arthropod repellent according to the present invention to the purified air Especially useful.
이하, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시하여 설명한다. 그러나 하기에 기술된 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제공되는 것일 뿐, 본 발명이 하기의 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described with reference to preferred embodiments. However, the examples described below are merely provided to more easily understand the present invention, and the present invention is not limited to the following examples.
실시예 Example
1. 재료 준비1. Material Preparation
신나모멈 카시아(Cinnamomum cassia), 로즈마리너스 오피시날리스(Rosmarinus officinalis), 유칼리투스 시트리오도라(Eucalyptus citriodora), 심보포곤 시트라투스(Cimbopogon citratus), 심보포곤 나르더스(Cimbopogon nardus) 및 바닐린은 미국 버제사(Berje, Bloomfield, NJ)로부터 구입하였고, 잔톡실럼 아르마툼(Zanthoxylum armatum)은 인도 시마 인터내셔널사(Seema International, Deli, India)에서 구입하였다. 그리고 DEET는 시그마-알드리치사(St. Louis, MO)에서 구입하였다. Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), crooked pogon Carry Traders (Cimbopogon nardus) and vanillin Was purchased from Berje, Bloomfield, NJ, and Zanthoxylum armatum was purchased from Seema International, Deli, India. And DEET were purchased from Sigma-Aldrich (St. Louis, Mo.).
상술한 재료 중 식물 정유에서 선택된 각각 1종과 바닐린을 에탄올에 녹여 조성물을 제조하였다. 식물 정유와 바닐린은 각각 5%:5%(v/w) 및 5%:15%(v/w)의 비율로 혼합하여, 에탄올에 녹임으로써 각각 최종 100㎕의 조성물을 수득하였다. Of the above materials, one selected from plant essential oil and vanillin, respectively, was dissolved in ethanol to prepare a composition. Plant essential oil and vanillin were respectively mixed at a ratio of 5%: 5% (v / w) and 5%: 15% (v / w), and dissolved in ethanol to obtain a final 100 μl of the composition, respectively.
한편 대조용으로 식물 정유 및 바닐린 대신 DEET를 포함하는 에탄올 용액을 각각 5%(v) 및 15%(v)로 준비하였다. Meanwhile, 5% (v) and 15% (v) of ethanol solutions containing DEET instead of plant essential oil and vanillin were prepared for control.
또한, 식물 정유 2종과 바닐린을 포함하는 조성물을 제조하기 위하여, 식물 정유 중에서 유칼리투스 시트리오도라, 심보포곤 시트라투스, 그리고 잔톡실럼 아르마툼에서 서로 다른 2종씩을 선택하여 바닐린과 함께 에탄올에 녹여 각각 100㎕의 조성물을 제조하였다. 식물 정유 2종과 바닐린의 혼합비는 각각 1:1:1, 3:1:1, 1:3:1 및 1:1:3이었고, 이는 5%:5%:5%, 15%:5%:5%, 5%:15%:5% 및 5%:5%:15%(v/v/w) 비율과 동일하다. 또한, 심보포곤 나르더스와 잔톡실럼 아르마툼 및 바닐린을 8.5:8.5:3의 비율로, 유칼리투스 시트리오도라, 잔톡실럼 아르마툼 및 바닐린을 8.5:8.5:3의 비율로 제조하였다(v/v/w). In addition, in order to prepare a composition comprising two plant essential oils and vanillin, two different plants from eucalyptus citridora, cymbopogon citratus, and xanthoxylum armatum are selected from plant essential oils in ethanol together with vanillin. Each 100 μl of the composition was prepared by melting. The mixing ratios of the two plant essential oils and vanillin were 1: 1: 1, 3: 1: 1, 1: 3: 1 and 1: 1: 3, respectively, which were 5%: 5%: 5% and 15%: 5%. Same as the 5%, 5%: 15%: 5%, and 5%: 5%: 15% (v / v / w) ratios. Furthermore, cymbopogon Narders and Xantoxilum Armatum and Vanillin were prepared in a ratio of 8.5: 8.5: 3, and Eucalyptus citridora, Xantoxilum Armatum and Vanillin were prepared in a ratio of 8.5: 8.5: 3 (v / v / w).
2. 실험 곤충 준비2. Experimental insect preparation
시험에 사용하기 위하여 에집트숲모기(Ae. aegypti)를 수년 간 전혀 살충제 노출 없이 실내에서 계대사육한 성충들로 이들을 마우스에 직접 흡혈하도록 하여 산란을 유도하였고 10%(w/v) 설탕물을 제공하여 부족한 탄수화물을 얻을 수 있도록 하였다. 유충은 플라스틱 트레이(24×35×5㎝)에 넣어 0.5g의 멸균 인공먹이(40-메쉬 치어사료 파우더/효모, 4:1, w/w)를 제공하였다. 이들을 26±2℃, 상대습도 70±5%로 하여 명암을 16:8 시간으로 하여 유지하였다.For use in testing, Egyptian mosquitoes ( Ae . Aegypti ) were passaged directly to mice with adult insects that had been passaged indoors for years without any pesticide exposure to induce spawning and provide 10% (w / v) sugar water. To get enough carbohydrates. The larvae were placed in a plastic tray (24 × 35 × 5 cm) to provide 0.5 g of sterile artificial feed (40-mesh fry powder / yeast, 4: 1, w / w). These were maintained at 26 ± 2 ° C. and relative humidity of 70 ± 5% with contrast as 16: 8 hours.
3. 실험3. Experiment
가. 피부시험법end. Skin test
모기 성충에 대한 본원발명에 따른 조성물의 기피력을 시험하기 위하여 상기 재료 준비에서 얻어진 조성물을 고무장갑 손등에 직경 5㎝의 구멍으로 노출된 시험자들의 피부 표면에 골고루 처리하여 피부시험을 수행하였다. 시험자들은 건강한 21-40세의 남성들이었다. 대조군은 에탄올 100㎕만을 처리하였다. In order to test the repellency of the composition according to the present invention for mosquitoes adults, the skin test was carried out by uniformly treating the skin surface of the tester exposed to the back of the rubber glove hand with a hole of 5 cm diameter in the preparation of the material. The test subjects were healthy 21-40 year old men. The control group treated only 100 μl of ethanol.
5-8일된 에집트숲모기 암컷 성충을 모기 성충 사육용 케이지(25×25×25 ㎤)에 250-260개체로 실험 개시 12-24시간 전 준비하였다. 대조구 팔뚝을 1분간 노출시켜 6개체 이상 앉거나 흡혈행동을 보이면 충분한 흡혈압(biting pressure)으로 판단하고 기피력 시험을 진행하였다. 30분 간격으로 상기 조성물들을 처리한 팔뚝을 3분 동안 노출시켜 모기 성충이 2초 이상 앉아 있거나 주둥이를 피부에 넣거나 흡혈 자리를 찾는 등의 흡혈행동을 연속으로 2개체 이상 보이면 그 실험을 중단하였다. 최초 물질을 처리한 시점과 2개체 이상이 연속으로 처리 부위에 앉거나 흡혈행동을 보인 시점까지를 완벽한 기피 지속 시간[complete protection time, CPT]으로 간주하였다.5-8 days old Egyptian mosquito female adults were prepared in 250-260 individuals in mosquito adult cages (25 × 25 × 25 cm 3) 12-24 hours before the start of the experiment. When the control forearm was exposed for 1 minute or more than 6 individuals sitting or exhibiting vampire behavior, it was judged as sufficient biting pressure and the evasion force test was performed. The experiment was discontinued if the forearm treated with the compositions was exposed for 3 minutes at intervals of 30 minutes and the adult mosquitoes were sitting for at least 2 seconds, or if more than two blood-sucking behaviors such as a snout was placed on the skin or a blood-sucking spot were found in succession. The complete protection time (CPT) was regarded as the time when the first material was treated and when two or more individuals sat in the treatment area or showed vampire behavior in succession.
완벽한 기피 지속 시간은 처리 시점과 최초의 흡혈이 이루어진 시간 사이를 의미하는 것으로 각 처리 평균간 비교를 위해 일반선형분석(GLM)을 실시하였다. 본페로니 다중검정법(Bonferoni's test, P=0.05)을 사용하여 평균간 비교를 하였다. The complete avoidance duration was defined as the time between treatment and the time of initial vampires. A general linear analysis (GLM) was performed for comparisons between treatment averages. Inter-mean comparisons were made using Bonferoni's test (P = 0.05).
그 결과는 표 1 내지 표 2에 나타내었다. 표 1은 식물 정유 및 바닐린 및 식물 정유 1종과 바닐린의 조성물의 에집트숲모기 성충에 대한 완벽한 기피 지속 시간(CPT)이다. 대조군으로, DEET 단독 처리의 에집트숲모기 성충에 대한 완벽한 기피 지속 시간(CPT)을 함께 기재한다. 표 2는 식물 정유 2종과 바닐린의 조성물의 에집트숲모기 성충에 대한 완벽한 기피 지속 시간(CPT)에 대한 결과이다. The results are shown in Tables 1-2. Table 1 shows the complete avoidance duration (CPT) for Egyptian mosquitoes adults of plant essential oils and vanillin and a composition of one plant essential oil and vanillin. As a control, complete evacuation duration (CPT) is described together for Egyptian adult mosquitoes in DEET alone. Table 2 shows the results for the complete avoidance duration (CPT) for the adult Egyptian mosquitoes in the composition of two plant essential oils and vanillin.
표 1
평균 완벽한 기피 지속 시간(CPT) (범위, 분)
바닐린 제외 바닐린 포함
5% 15% 5% : 5% 5% : 15% 15%:5% 15%:15%
신나모멈 카시아 75(60-90) -* 135(120-150) 157.5(120-180) -* -*
로즈마리너스 오피시날리스 0(0) 60(60) 52.5(30-60) 112.5(60-150) 60(30-90) 135(120-150)
유칼리투스 시트리오도라 22.5(0-30) 52.5(30-60) 60(60) 67.5(30-120) 75(60-90) 90(60-120)
심보포곤 시트라투스 30(30) 37.5(30-60) 105(90-120) 97.5(60-120) 105(90-120) 150(150)
잔톡실럼 아르마툼 30(30) 75(60-90) 60(60) 90(60-120) 75(60-90) 120(90-150)
심보포곤 나르더스 25(25) 70(60-80) 55(55) 85(50-120) 70(60-80) 105(85-125)
바닐린 15(0-30_ 37.5(0-60)
DEET 127.5(120-150) 247.5(240-270)
Table 1
Average Perfect Evasion Duration (CPT) (Range, Minutes)
Except vanillin Contains vanillin
5% 15% 5%: 5% 5%: 15% 15%: 5% 15%: 15%
Cinnamomum Cassia 75 (60-90) - * 135 (120-150) 157.5 (120-180) - * - *
Rose Mariners Officinalis 0 (0) 60 (60) 52.5 (30-60) 112.5 (60-150) 60 (30-90) 135 (120-150)
Eucalyptus citrudora 22.5 (0-30) 52.5 (30-60) 60 (60) 67.5 (30-120) 75 (60-90) 90 (60-120)
Cympogon Cilatus 30 (30) 37.5 (30-60) 105 (90-120) 97.5 (60-120) 105 (90-120) 150 (150)
Xantoxilum Armatum 30 (30) 75 (60-90) 60 (60) 90 (60-120) 75 (60-90) 120 (90-150)
Symbogongon Narders 25 (25) 70 (60-80) 55 (55) 85 (50-120) 70 (60-80) 105 (85-125)
vanillin 15 (0-30_ 37.5 (0-60)
DEET 127.5 (120-150) 247.5 (240-270)
* 피부자극성이 강해서 실험치 구하지 못함 * Due to strong skin irritation, experimental value cannot be obtained
상기 표 1에서 알 수 있듯이, 신나모멈 카시아 5% 단독 처리시 75분의 CPT를 나타내었고, 심보포곤 시트라투스와 잔톡실럼 아르마툼은 30분 그리고 유칼리투스시트리오도라 및 바닐린은 각각 22.5분과 15분, 로즈마리너스 오피시날리스는 효과를 나타내지 않았다. 반면 DEET는 127.5분으로 가장 강력한 기피력을 나타냈고 15%에서는 247.5분으로 향상된 결과를 나타냈다. 반면에, 상기 표 1에서 확인할 수 있듯이, 식물 정유와 바닐린을 포함하는 조성물의 경우가, 각각을 단독으로 사용하였을 때와 비교하여 그 기피력이 시너지 효과를 내면서 증진됨을 알 수 있었다. 이러한 기피력의 증진은 각 식물 정유에 바닐린을 5%와 15% 혼합 처리한 경우, CPT가 급격히 상승한다는 사실을 통해 알 수 있었다(F=71.75, P<0.0001). 이와 같이 식물 정유의 농도 및 바닐린 혼합이라는 두 가지 인자는 에집트숲모기의 기피력에 유의미하게 중요하다는 것을 알 수 있었다(F=5.61, P<0.0001).As can be seen in Table 1, Cinnamomum cassia 5% alone treatment showed a 75-minute CPT, cymbopogon citratus and xantoxilum armatum 30 minutes and eucalyptus citridora and vanillin 22.5 minutes and At 15 minutes, Rosemarinus Offinalis had no effect. On the other hand, DEET showed the strongest evasion at 127.5 minutes and improved to 247.5 minutes at 15%. On the other hand, as can be seen in Table 1, the composition containing the plant essential oil and vanillin, it was found that the repelling power is improved while having a synergistic effect compared to when using each alone. This increase in repellency was shown by the fact that CPT increased rapidly when vanillin was mixed with 5% and 15% of each plant essential oil (F = 71.75, P <0.0001). Thus, two factors, the concentration of plant essential oil and vanillin mixture, were found to be significantly important for the repellent force of Egyptian forest mosquitoes (F = 5.61, P <0.0001).
한편, 식물 정유 2종 및 바닐린을 혼합하여 본 발명에 따르는 기피제 조성물을 제조하여, 동일한 실험을 진행하였다. 그 결과를 표 2에 기재하였다. Meanwhile, two plant essential oils and vanillin were mixed to prepare a repellent composition according to the present invention, and the same experiment was conducted. The results are shown in Table 2.
표 2
그룹a CPT 평균(범위, 분)
1:1:1 3:1:1 1:3:1 1:1:3
A 105(90-120) 165(150-180) 165(120-210) 165(150-180)
B 142.5(90-180) 82.5(60-120) 150(150) 240(210-300)
C 150(120-180) 210(180-240) 270(210-330) 240(180-300)
D 140(110-170) 200(170-230) 240(180-300) 220(150-290)
TABLE 2
Group a CPT average (range, minutes)
1: 1: 1 3: 1: 1 1: 3: 1 1: 1: 3
A 105 (90-120) 165 (150-180) 165 (120-210) 165 (150-180)
B 142.5 (90-180) 82.5 (60-120) 150 (150) 240 (210-300)
C 150 (120-180) 210 (180-240) 270 (210-330) 240 (180-300)
D 140 (110-170) 200 (170-230) 240 (180-300) 220 (150-290)
a 그룹 A는 유칼리투스 시트리오도라: 심보포곤 시트라투스:바닐린A is a group of eucalyptus Rio Dora Tooth sheet: pogon Citra crooked tooth: vanillin
그룹 B는 유칼리투스 시트리오도라:잔톡실럼 아르마툼:바닐린Group B is Eucalyptus Citridora: Xantoxilum Armatum: Vanillin
그룹 C는 심보포곤 시트라투스:잔톡실럼 아르마툼:바닐린Group C is Cymbopogon Cilatus: Xantoxilum Armatum: Vanillin
그룹 D는 심보포곤 나르더스:잔톡실럼 아르마툼:바닐린Group D is Simbopogon Narders: Xantoxilum Armatum: Vanillin
상기 표 2에서 알 수 있는 바와 같이, 식물 정유 2종과 바닐린 혼합은 단일 오일과 바닐린 혼합에 비해 더욱 월등하게 향상된 기피력(완벽한 기피 지속 시간, CPT)을 나타냈다. 이러한 효과는 이들 조성물의 각 구성성분들의 단순한 혼합에 의해 기피 지속 시간이 증가한 것 이상의 시너지 효과를 발생하는 것을 알 수 있다. 즉, 표 2의 결과에서 얻은 각 조성물 성분들의 1:1:1 기준치를 CPT에 근간하여 비교해 보면, 그룹 A에 속하는 심보포곤 시트라투스는 30분, 유칼리투스 시트리오도라는 22.5분, 바닐린은 15분으로 이들을 산술적으로 합산한 CPT는 단지 67.5분을 기대할 수 있으나, 상기 표 2의 실제 조성물을 통해 얻어진 실험적인 결과는 기대치보다 약 2배 증가된 105분임을 알 수 있다. 즉 본 발명에 따르는 흡혈 절지 동물용 기피제 조성물은 식물 정유 2종 및 바닐린의 혼합으로 인한 시너지 효과를 가짐을 확인할 수 있었다. As can be seen in Table 2, the two plant essential oils and vanillin blends showed significantly better repellency (perfect evacuation duration, CPT) compared to a single oil and vanillin blend. It can be seen that this effect results in synergies beyond that of increased repellency duration by simple mixing of each component of these compositions. That is, when comparing the 1: 1: 1 reference value of each composition component obtained from the results of Table 2 based on CPT, Cympogon citratus belonging to group A is 30 minutes, 22.5 minutes eucalyptus citriodor, and vanillin Arithmetic summation of these at 15 minutes can only expect 67.5 minutes, but the experimental results obtained with the actual composition of Table 2 can be seen that 105 minutes, which is about 2 times higher than expected. That is, the blood repellent arthropod repellent composition according to the present invention was confirmed to have a synergistic effect due to the mixing of two plant essential oils and vanillin.
나. 케이지 시험법I. Cage test method
본 시험은 시험계 중앙에 벽체로서 가로놓여 있고 중앙에 10㎝ 구멍이 뚫린 두꺼운 카드판(건물 창문)을 통과하여 처리 구역 내로 모기가 침입하여 들어오는지를 검정하기 위한 것이다. 또한, 본 시험은 비드제 제형의 시간의 경과에 따른 평균 저지력을 검정하기 위한 것이다. This test is to test whether mosquitoes invade the treatment area through a thick card board (building window) that is laid horizontally in the center of the test system and has a 10 cm hole in the center. In addition, this test is to assay the average stopping power over time of the bead formulation.
본 시험에서, 본 발명에 따르는 조성물은 5㎕를 여지에 처리하거나, 비드제를 제조하여 사용하였다. 상기 비드제는 다공성 셀룰로오스 비드인 비스코펄(3.8~4.2mm, Rengo 사, Tokyo, Japan)에 본 발명에 따르는 기피제 조성물을 담지하여 제조하였다. 수득된 비스코펄 비드제 1g에서 조성물은 약 0.465g이고, 비스코펄의 무게가 약 0.535g이었다. 사용되는 조성물의 각 성분간의 비율은 하기 표에 정리되어 있다. In this test, the compositions according to the invention were treated with 5 μl as needed or prepared beads. The bead was prepared by supporting the repellent composition according to the present invention in a biscopearl (3.8-4.2 mm, Rengo, Tokyo, Japan), which is a porous cellulose bead. In 1 g of the biscopearl bead agent obtained, the composition was about 0.465 g, and the weight of the biscopearl was about 0.535 g. The ratio between each component of the composition used is summarized in the following table.
케이지 시험을 수행하기 위해, 성충이 된지 5-10일 된 에집트숲모기 암컷 100개체를 사용하였고, 시험 개시 2시간 전에 준비하였다. 시험용 케이지는 도 1에 예시되어 있다. 상기 케이지는 철제 프레임으로 물질 처리 구역(1), 무처리 구역(2) 및 방사 케이지(3)를 제조하고, 물질 처리 구역(1) 및 방사 케이지(3)는 폴리에스터 네트로 감싸고, 무처리 구역(2)은 아크릴 판을 대었다. 무처리 구역(2)의 중앙에 지름 10 cm의 구멍을 뚫은 두꺼운 카드판을 설치하였다. 상기 물질 처리 구역(1)에 모기 유인원으로 마우스 1개체를 철망에 고정하여 움직일 수 없도록 시험 개시 10분 전에 미리 넣어두고 본 발명에 따르는 기피제 조성물을 처리한 여지 및 비드제를 물질 처리 구역(1)에 놓았다. 조성물을 처리한 여지의 경우는 1분 동안 자연 건조시켜 물질 처리 구역(1)의 상단에 실로 매달아 고정하고 3분 동안 휘발되도록 하였다. 그 후, 방사 케이지(3)에 준비한 에집트숲모기 암컷 성충들을 방사하였다. 본 시험에서 모기의 자연적인 이동 흐름은 모기 방사 케이지(3)로부터 물질 처리 구역(1)이다.To perform the cage test, 100 female Egyptian mosquitoes, 5-10 days old, were used and prepared 2 hours before the start of the test. The test cage is illustrated in FIG. 1. The cage is made of a steel frame to produce a material treatment zone (1), an untreated zone (2) and a spinning cage (3), the material handling zone (1) and a spinning cage (3) are wrapped in a polyester net, and the untreated zone (2) put an acrylic plate. In the center of the non-treatment zone 2, a thick card board having a hole of 10 cm in diameter was installed. 10 minutes before the start of the test so that one mouse as a mosquito ape with a mosquito ape is immobilized in the material processing zone 1, and the room for treating the repellent composition according to the present invention and the bead agent are placed in the material processing zone (1). Put on. For the case where the composition was treated, it was allowed to dry naturally for 1 minute and suspended by thread on top of the material treatment zone 1 and allowed to volatilize for 3 minutes. Thereafter, female adult female mosquitoes in the spinning cage 3 were spun. The natural flow of mosquitoes in this test is the material handling zone (1) from the mosquito spinning cage (3).
물질 처리 구역(1) 내로 모기의 유입을 저지하는 평균 저지율은 하기 식을 이용하여 계산하였다. The average rate of inhibition for preventing the ingress of mosquitoes into the material treatment zone 1 was calculated using the following formula.
저지율(%)={(30-처리 구역에 침투한 모기수)/30}×100% Blockage = {(Mosquitoes penetrated into 30-treatment zone) / 30} × 100
그 결과는 표 3에 나타내었다. The results are shown in Table 3.
표 3
평균 저지율(%)
30분 60분 90분 120분
A:B:D = 1:1:1 조성물 5㎕를 여지에 처리 90 84 84 80
A:C:D = 1:1:1 95 92 92 84
B:C:D = 1:1:1 92 92 92 89
A:B:D = 3:1:1 96 96 91 92
A:C:D = 3:1:1 84 80 80 72
B:C:D = 3:1:1 100 98 98 96
A:B:D = 1:3:1 96 94 94 92
A:C:D = 1:3:1 95 94 92 92
B:C:D = 1:3:1 100 98 98 98
A:B:D = 1:1:3 92 92 86 82
A:C:D = 1:1:3 100 100 99 98
B:C:D = 1:1:3 100 98 98 96
A:B:D = 1:1:1 비드제로 제조하여, 비스코펄 1g 처리 92 91 84 84
A:C:D = 1:1:1 96 94 89 84
B:C:D = 1:1:1 96 92 90 90
A:B:D = 3:1:1 92 92 88 81
A:C:D = 3:1:1 89 81 77 73
B:C:D = 3:1:1 98 98 91 88
A:B:D = 1:3:1 89 84 84 78
A:C:D = 1:3:1 91 84 84 81
B:C:D = 1:3:1 100 94 91 90
A:B:D = 1:1:3 94 92 87 82
A:C:D = 1:1:3 98 98 94 91
B:C:D = 1:1:3 100 96 96 94
TABLE 3
Average Block Rate (%)
30 minutes 60 minutes 90 minutes 120 minutes
A: B: D = 1: 1: 1 5 μl of composition is treated here 90 84 84 80
A: C: D = 1: 1: 1 95 92 92 84
B: C: D = 1: 1: 1 92 92 92 89
A: B: D = 3: 1: 1 96 96 91 92
A: C: D = 3: 1: 1 84 80 80 72
B: C: D = 3: 1: 1 100 98 98 96
A: B: D = 1: 3: 1 96 94 94 92
A: C: D = 1: 3: 1 95 94 92 92
B: C: D = 1: 3: 1 100 98 98 98
A: B: D = 1: 1: 3 92 92 86 82
A: C: D = 1: 1: 3 100 100 99 98
B: C: D = 1: 1: 3 100 98 98 96
A: B: D = 1: 1: 1 Manufactured with beads and treated with 1 g of biscopearl 92 91 84 84
A: C: D = 1: 1: 1 96 94 89 84
B: C: D = 1: 1: 1 96 92 90 90
A: B: D = 3: 1: 1 92 92 88 81
A: C: D = 3: 1: 1 89 81 77 73
B: C: D = 3: 1: 1 98 98 91 88
A: B: D = 1: 3: 1 89 84 84 78
A: C: D = 1: 3: 1 91 84 84 81
B: C: D = 1: 3: 1 100 94 91 90
A: B: D = 1: 1: 3 94 92 87 82
A: C: D = 1: 1: 3 98 98 94 91
B: C: D = 1: 1: 3 100 96 96 94
A = 유칼리투스 시트리오도라A = eucalyptus citridora
B = 심보포곤 시트라투스B = cymbopogon citratus
C = 잔톡실럼 아르마툼C = Xantoxilum Armatum
D = 바닐린D = vanillin
상기한 표 3에서 확인할 수 있듯이, 표 3에 개시된 조성비로 제조된 조성물을 여지에 직접 처리하여 처리 구역 내로 모기 유입 저지력을 조사한 결과, 시험 120분 이후에도 유칼리투스 시트리오도라: 잔톡실럼 아르마툼: 바닐린을 3:1:1 비율로 혼합한 조성물을 여지에 처리한 경우를 제외한 모든 조성물들이 80% 이상의 모기 침투를 저지할 수 있었다. As can be seen in Table 3 above, the composition prepared in the composition ratio disclosed in Table 3 was directly treated to investigate the mosquito inflow inhibition into the treatment zone, and even after 120 minutes, eucalyptus citridora: xanthoxylum armatum: vanillin All compositions were able to block more than 80% of mosquito infiltration, except when the composition was mixed at a 3: 1: 1 ratio.
또한 표 3에 개시된 조성비로 제조된 조성물을 비스코펄(크기: 3.8~4.2mm, Rengo 사, Tokyo, Japan)에 담지하여 조제한 비드제 1g을 처리한 경우에도, 거의 모든 비드제들이 80% 이상의 모기 침투를 저지하는 효과를 나타내었다. 따라서, 본 발명에 따르는 조성물의 유효 성분이 일정 농도 이상 공간에 분포되어 있으면, 이를 모기가 인지하게 되어, 기피 물질이 발향되는 공간으로의 침투를 기피한다는 것을 알 수 있었다. In addition, even when treated with 1 g of the bead agent prepared by supporting the composition prepared in the composition ratios shown in Table 3 in biscopearl (size: 3.8-4.2 mm, Rengo, Tokyo, Japan), almost all the bead agents were at least 80% mosquitoes. The effect of retarding penetration was shown. Therefore, when the active ingredient of the composition according to the present invention is distributed in a space above a certain concentration, it was found that the mosquito is recognized, avoiding the penetration into the space where the repellent material is oriented.
다. 챔버 시험All. Chamber test
넓은 공간에서의 본 발명의 조성물의 기피력을 알아보기 위해 챔버 시험을 수행하였다. 시험에 사용되는 챔버(1.2 X 1.2 X 1.2m)는 철제 프레임에 아크릴판으로 덮어 제조하였고, 전면의 아래쪽에 정사각형 구멍(20 X 20cm)를 내어 설치하였다. 상기 정사각형의 구멍을 10개의 구멍(각 구멍은 1.5 X 1.5cm)을 구비한 검정색 카드판으로 막았다. 모기 방사 케이지(25 X 25 X 25cm)를 정사각형 구멍에 연결하였다. 또 다른 수집 케이지를 챔버 내에 위치시켰다. 방사 케이지 및 수집 케이지는 카드판으로 연결되고, 모두 폴리에스터 네트로 감쌌다. 팬(모델명: EFB-2-3, 한일(Hanil), Korea)을 작동시켜 성분의 확산을 촉진시켰다. 모기 유인원로서 인간 지원자 1명이 챔버 내에 들어갔다. 대조군으로 기피 물질이 없고 모기 유인원만 위치한 상태에서 2 시간 동안 챔버 안으로 유입되는 모기의 개체수를 계수하여 사용한다. 기피제로는 하술되는 비드제가 담긴 모듈을 사용하였다. 유인물질 처리는 시험 30분 전, 기피제 처리는 시험 1시간 전에 수행하였다. 반복 시험을 하여 통계처리를 통한 결과를 수득하였다. Chamber tests were performed to determine the repellency of the compositions of the present invention in large spaces. The chamber used for the test (1.2 X 1.2 X 1.2 m) was manufactured by covering the steel frame with an acrylic plate and installing a square hole (20 X 20 cm) at the bottom of the front side. The square holes were closed with a black card plate with ten holes (each hole 1.5 X 1.5 cm). A mosquito spinning cage (25 × 25 × 25 cm) was connected to the square hole. Another collection cage was placed in the chamber. The spinning cage and the collecting cage were connected with card boards and all wrapped with polyester nets. The fan (model name: EFB-2-3, Hanil, Korea) was operated to promote the diffusion of the components. As a mosquito ape, one human volunteer entered the chamber. With no repellent and only mosquito apes, 2 Count the number of mosquitoes entering the chamber over time. As a repellent, a module containing the beads described below was used. Attractant treatment was performed 30 minutes before the test and repellent treatment was performed 1 hour before the test. Repeated tests were performed to obtain results through statistical treatment.
비드제는 케이지 시험법에 사용된 것과 동일하다. 상기 비드제에 담지되는 조성물은 심포보곤 시트라투스:잔톡실럼 아르마툼:바닐린을 8.5:8.5:3(v/v/w)의 비율로 제조한 조성물(용매로 에탄올 사용)이다. Beading agents are the same as those used in the cage assay. The composition supported on the bead is a composition (using ethanol as a solvent) in which a ratio of symbobogon citratus: xanthoxilum armatum: vanillin is 8.5: 8.5: 3 (v / v / w).
한편, 본 실험을 위해 도 2에 기재된 모양의 모듈을 제조하였다. 상기 모듈에 개폐를 조절할 수 있는 10개의 구멍을 만들고, 상기 구멍에 각각 동일한 양의 비드제를 담고, 따라서, 하나의 모듈에 총 10 내지 11g 정도의 비드제가 담기고, 상기 비드제 내부에는 대략 4.7 내지 5.4g의 조성물이 담지된다. On the other hand, the module of the shape described in Figure 2 was prepared for this experiment. Make 10 holes to control the opening and closing of the module, each containing the same amount of beading agent, so that a total of about 10 to 11g of beading agent in one module, and approximately 4.7 inside the beading agent To 5.4 g of the composition is supported.
상술한 챔버 내에 10개 구멍이 완전 개방된 모듈 2세트를 위치시키고, 성충이 된지 5~10일이 된 120개체의 에집트숲 암컷 모기를 사용하였다. 챔버 내로 침입하는 암컷 모기의 수를 팬 작동 후 5분, 10분, 20분 및 30분에 계수하였다. 기피력은 각 시간별 아무런 처리를 하지 않을 시 들어오는 모기수(대조군)와 본 발명의 조성물 처리시의 들어오는 모기수를 계수하여 그 비율로 퇴치율을 계산하였고, 수득되는 결과를 평균하여 최종 퇴치율로 확정하였다. 단위는 퍼센트(%)이다. Two sets of ten fully open modules were placed in the chamber described above, and 120 Egyptian forest female mosquitoes were used 5 to 10 days old. The number of female mosquitoes entering the chamber was counted at 5, 10, 20 and 30 minutes after fan operation. Repelling power was calculated by counting the number of incoming mosquitoes (control) and the number of incoming mosquitoes during treatment of the composition of the present invention when no treatment was performed for each time, and calculated the repelling rate by the ratio, and averaged the obtained results as the final repelling rate Confirmed. The unit is percent.
시간에 따른 기피율을 3회 반복 수행하여 평균적인 기피율을 수득하였다. 수득되는 기피력의 결과는 표 4에 기재하였다.The evasion rate with time was repeated three times to obtain an average evasion rate. The results of the obtained repellent force are shown in Table 4.
수득되는 기피력의 결과는 표 4에 기재하였다. The results of the obtained repellent force are shown in Table 4.
표 4
기피력 (평균±SE)
No. 5분 10분 20분
1 66.32±11.35 77.73±8.89 67.81±12.83
2 100±2.1 81.94±1.13 80.56±13.71
3 76.36±8.18 64.22±5.87 64.41±4.50
4 80.56±10.02 55.99±13.04 62.15±8.22
Table 4
Evasion force (mean ± SE)
No. 5 minutes 10 minutes 20 minutes
One 66.32 ± 11.35 77.73 ± 8.89 67.81 ± 12.83
2 100 ± 2.1 81.94 ± 1.13 80.56 ± 13.71
3 76.36 ± 8.18 64.22 ± 5.87 64.41 ± 4.50
4 80.56 ± 10.02 55.99 ± 13.04 62.15 ± 8.22
1. 심보포곤 나르더스 : 잔톡실럼 아르마툼 : 바닐린 = 8.5:8.5:3 (v/v/w) 1.Symbopogon Narders: Xantoxilum Armatum: Vanillin = 8.5: 8.5: 3 (v / v / w)
2. 심보포곤 시트라투스 : 잔톡실럼 아르마툼 : 바닐린 = 8.5:8.5:3 (v/v/w)2. Combopogon citratus: Xantoxilum Armatum: Vanillin = 8.5: 8.5: 3 (v / v / w)
3. 심보포곤 나르더스 : 잔톡실럼 아르마툼 : 바닐린 = 1:1:1 (v/v/w)3. Simbopogon Narders: Xantoxilum Armatum: Vanillin = 1: 1: 1 (v / v / w)
4. 심보포곤 시트라투스 : 잔톡실럼 아르마툼 : 바닐린 = 1:1:1 (v/v/w)4. Combopogon citratus: Xantoxilum Armatum: Vanillin = 1: 1: 1 (v / v / w)
상기 표 4로부터, 넓은 공간에서도 본 발명에 따르는 모기 기피제의 기피력이 우수하고, 특히 심보포곤 시트라투스 : 잔톡실럼 아르마툼 : 바닐린 = 8.5:8.5:3 (v/v/w)인 조성물이 기피력이 우수하다는 사실을 확인할 수 있다. From the above Table 4, the composition of the mosquito repellent according to the present invention is excellent even in a large space, in particular the cymbopogon citratus: xanthoxylum armatum: vanillin = 8.5: 8.5: 3 (v / v / w) It can be confirmed that the repellent is excellent.
한편, 심보포곤 시트라투스 : 잔톡실럼 아르마툼 : 바닐린 = 8.5:8.5:3 (v/v/w)인 조성물의 기피율과 사용수명을 확인하고자, 시간의 흐름에 따른 기피력의 변화 추이를 상기 챔버 시험을 실시하여 관찰하였다. 수득되는 결과를 하기 표 5에 기재하였다. Meanwhile, in order to check the evasion rate and the service life of the composition of Cymbopogon citratus: Xantoxilum Armatum: vanillin = 8.5: 8.5: 3 (v / v / w), the change of the evasion force over time was examined. The chamber test was conducted and observed. The results obtained are shown in Table 5 below.
표 5
반복수 처리 후 시간(분)
10분 30분 60분
1 100 93.3 93.3
2 87.5 72.7 -
3 100 66.7 70
기피력(%, 평균±SE)
평균 95.8±4.2 77.6±8 81.7±11.7
Table 5
Iterations Post Processing Minutes
10 minutes 30 minutes 60 minutes
One 100 93.3 93.3
2 87.5 72.7 -
3 100 66.7 70
Evasion (%, mean ± SE)
Average 95.8 ± 4.2 77.6 ± 8 81.7 ± 11.7
상기 표 5에서 알 수 있듯이, 전체 평균 83% 이상의 기피율을 보이며, 최소 60분 동안 70% 이상의 기피율을 갖는 것을 확인할 수 있다. As can be seen in Table 5, the overall average shows a avoidance rate of 83% or more, it can be confirmed that having a avoidance rate of 70% or more for at least 60 minutes.
라. 가속 시험la. Acceleration test
본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물의 기피력의 지속가능기간을 가속 시험으로 측정하였다. 흡혈성 절지 동물용 기피제 조성물은 평균적으로 하루 8시간 정도 사용하고 챔버 시험에서 사용되는 챔버 내의 벽에 모기가 이동할 수 있는 구멍은 10개이지만, 가속 시험으로 위해 24시간 연속하여 시험을 지속하고, 챔버 내의 벽의 구멍을 40개로 하였다. 본 발명에 따르는 흡혈성 절지 동물용 기피제 조성물의 지속력이 90일 동안 지속되는지 알기 위하여, 상기 조건 하에서 8일 동안 가동한 이후 시간의 경과에 따른 기피력의 효과를 측정하였다. The sustained duration of the repellency of the repellent composition for blood-sucking arthropods according to the present invention was measured by an accelerated test. Vampire repellent compositions for vampire arthropods use on average about 8 hours a day and have 10 holes through which mosquitoes can move on the walls in the chamber used in the chamber test, but the test is continued for 24 hours continuously for the accelerated test. Inner wall was made into 40 holes. In order to know if the persistence of the repellent composition for blood-absorbing arthropods according to the present invention lasts for 90 days, the effect of the repellency over time after the operation for 8 days under the above conditions was measured.
구멍을 40개로 늘린 것을 제외하고는 상술한 챔버 시험과 동일한 챔버를 사용하여 시험을 진행하였으며, 대조군은 에탄올 용매만 처리하였고, 실험군은 심보포곤 시트라투스 : 잔톡실럼 아르마툼 : 바닐린 = 8.5:8.5:3 (v/v/w)인 조성물(용매는 에탄올)을 처리하였다. 수득되는 결과를 하기 표 6에 기재하였다. The test was conducted using the same chamber as the above chamber test except that the holes were increased to 40, and the control group was treated only with ethanol solvent, and the experimental group was simbopogon citratus: xanthoxylum armatum: vanillin = 8.5: 8.5 The composition (solvent is ethanol) of: 3 (v / v / w) was treated. The results obtained are shown in Table 6 below.
표 6
유입량 초기 10분 40분 50분 60분 평균
대조군(마리) 0 12 13 14 12 12.75
실험군(마리) 0 5 7 4 4 5
기피력(%) 0 58.3 46.2 71.4 66.7 60.8
Table 6
inflow Early 10 minutes 40 minutes 50 minutes 60 minutes Average
Control group (mari) 0 12 13 14 12 12.75
Experiment group (mari) 0 5 7 4 4 5
Evasion (%) 0 58.3 46.2 71.4 66.7 60.8
상기 표 6으로부터, 본 발명에 따르는 조성물을 담지한 비드제 처리 시, 평균 60% 이상의 기피 효과가 90일 이상 지속되는 것을 확인할 수 있었다. From Table 6, when the bead treatment carrying the composition according to the present invention, it was confirmed that the average repellent effect of 60% or more lasts more than 90 days.

Claims (9)

  1. 신나모멈 카시아(Cinnamomum cassia), 로즈마리너스 오피시날리스(Rosmarinus officinalis), 유칼리투스 시트리오도라(Eucalyptus citriodora), 심보포곤 시트라투스(Cimbopogon citratus), 잔톡실럼 아르마툼(Xanthoxylum armatum) 및 심보포곤 나르더스(Cymbopogon nardus)로 이루어진 그룹으로부터 선택되는 1종의 식물 정유 및 바닐린(vanillin)을 포함하는 흡혈성 절지 동물용 기피제 조성물.Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Xanthoxylum armatum) and crooked A repellent composition for blood-sucking arthropods comprising one plant essential oil and vanillin selected from the group consisting of Cymbopogon nardus .
  2. 제 1 항에 있어서, The method of claim 1,
    식물 정유와 바닐린의 조성이 (1 내지 3) : (1 내지 3)의 비율(v/w)인 흡혈성 절지 동물용 기피제 조성물. The repellent composition for blood-absorbing arthropods whose composition of plant essential oils and vanillin is a ratio (v / w) of (1-3): (1-3).
  3. 신나모멈 카시아(Cinnamomum cassia), 로즈마리너스 오피시날리스(Rosmarinus officinalis), 유칼리투스 시트리오도라(Eucalyptus citriodora), 심보포곤 시트라투스(Cimbopogon citratus), 잔톡실럼 아르마툼(Xanthoxylum armatum) 및 심보포곤 나르더스(Cymbopogon nardus)로 이루어진 그룹으로부터 선택된 2종의 식물 정유 및 바닐린(vanillin)을 포함하는 흡혈성 절지 동물용 기피제 조성물.Cinnamoyl stop Cassia (Cinnamomum cassia), rosemary bonus operational during the day lease (Rosmarinus officinalis), Eucalyptus tooth sheet Rio Dorado (Eucalyptus citriodora), crooked pogon Citra tooth (Cimbopogon citratus), jantok silreom Armadillo Tomb (Xanthoxylum armatum) and crooked A repellent composition for blood-sucking arthropods comprising two plant essential oils selected from the group consisting of Cymbopogon nardus and vanillin.
  4. 제 3 항에 있어서, The method of claim 3, wherein
    식물 정유: 또 다른 식물 정유: 바닐린의 조성이 (1 내지 3) : (1 내지 3) : (1 내지 3)의 비율(v/v/w)인 흡혈성 절지 동물용 기피제 조성물.Plant essential oil: Another plant essential oil: A blood repellent arthropod repellent composition in which the composition of vanillin is (1 to 3): (1 to 3): (1 to 3) (v / v / w).
  5. 제 4 항에 있어서, The method of claim 4, wherein
    식물 정유: 또 다른 식물 정유: 바닐린의 조성이 1:1:1, 3:1:1, 1:3:1, 1:1:3 또는 8.5:8.5:3의 비율(v/v/w)인 흡혈성 절지 동물용 기피제 조성물.Plant Essential Oil: Another Plant Essential Oil: The composition of vanillin is 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3 (v / v / w) Phospholipid repellent arthropod repellent composition.
  6. 제 3 항에 있어서,The method of claim 3, wherein
    잔톡실럼 아르마툼(Xanthoxylum armatum), 심보포곤 시트라투스(Cimbopogon citratus) 및 바닐린을 포함하는 흡혈성 절지 동물용 기피제 조성물.A repellent composition for vampire arthropods comprising xanthoxylum armatum , cimbopogon citratus and vanillin.
  7. 제 3 항에 있어서,The method of claim 3, wherein
    잔톡실럼 아르마툼(Xanthoxylum armatum), 심보포곤 나르더스(Cymbopogon nardus) 및 바닐린을 포함하는 흡혈성 절지 동물용 기피제 조성물.A repellent composition for vampire arthropods comprising xanthoxylum armatum , cymbopogon nardus and vanillin.
  8. 제1항 내지 제7항 중 어느 한 항에 따른 흡혈성 절지 동물용 기피제 조성물을 포함하는 다공성 담체. A porous carrier comprising the repellent composition for a blood-sucking arthropod according to any one of claims 1 to 7.
  9. 제1항 내지 제7항 중 어느 한 항에 따른 흡혈성 절지 동물용 기피제 조성물을 포함하는 기피제 발생장치.Repellent generating device comprising the repellent composition for blood-sucking arthropod according to any one of claims 1 to 7.
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