WO2012078223A1 - Nouveau procédé pour produire un hydrolysat de protéine marine à contamination en métaux lourds ultra-faible - Google Patents
Nouveau procédé pour produire un hydrolysat de protéine marine à contamination en métaux lourds ultra-faible Download PDFInfo
- Publication number
- WO2012078223A1 WO2012078223A1 PCT/US2011/051315 US2011051315W WO2012078223A1 WO 2012078223 A1 WO2012078223 A1 WO 2012078223A1 US 2011051315 W US2011051315 W US 2011051315W WO 2012078223 A1 WO2012078223 A1 WO 2012078223A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- heavy metal
- protein hydrolysate
- marine protein
- fish
- process according
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 36
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 26
- 238000011109 contamination Methods 0.000 title claims abstract description 9
- 239000000413 hydrolysate Substances 0.000 title description 3
- 239000003531 protein hydrolysate Substances 0.000 claims abstract description 26
- 108010009736 Protein Hydrolysates Proteins 0.000 claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 23
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 22
- 241000251468 Actinopterygii Species 0.000 claims abstract description 19
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 239000000356 contaminant Substances 0.000 claims abstract description 10
- 150000002632 lipids Chemical class 0.000 claims abstract description 10
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 16
- 230000007062 hydrolysis Effects 0.000 claims description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 239000006227 byproduct Substances 0.000 claims description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 2
- 229910001873 dinitrogen Inorganic materials 0.000 claims 1
- 239000011552 falling film Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 238000001035 drying Methods 0.000 abstract description 6
- 238000001704 evaporation Methods 0.000 abstract description 6
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 5
- 239000002245 particle Substances 0.000 abstract description 5
- 239000012223 aqueous fraction Substances 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000019688 fish Nutrition 0.000 description 17
- 239000012071 phase Substances 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 5
- 210000000988 bone and bone Anatomy 0.000 description 5
- 229910052753 mercury Inorganic materials 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000007065 protein hydrolysis Effects 0.000 description 5
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 4
- 229910052785 arsenic Inorganic materials 0.000 description 4
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 description 3
- 108010028690 Fish Proteins Proteins 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 231100000770 Toxic Equivalency Factor Toxicity 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000011261 inert gas Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 150000003071 polychlorinated biphenyls Chemical class 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 235000014102 seafood Nutrition 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001269252 Domingoa Species 0.000 description 1
- 108010091443 Exopeptidases Proteins 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 101710118538 Protease Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 235000003441 saturated fatty acids Nutrition 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 239000004550 soluble concentrate Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012258 stirred mixture Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/348—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins obtained from waste materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- This invention relates to a new method to produce soluble marine protein hydro lysate powder with ultra-low heavy metal contamination. More specifically, this invention relates to a production method that comprises occluding the heavy metal contaminants present in a soluble marine protein hydrolysate solution produced by enzymatic hydrolysis of industrial fish or fish off-cuts, into a lipid particulate structure and separating these particles from the aqueous protein layer. This invention further relates to a method for separating the fatty particulates from the soluble marine protem hydrolysate aqueous layer using a continuously operating automatic disc scrapper or membrane filtering system and evaporating and drying this aqueous fraction into a healthier and safer protein hydrolysate powder for direct and indirect human consumption.
- Enzymatic processes have shown distinct advantages to other hydrolysis methods allowing for milder conditions and less power consumption which result in a less bitter and easily digestible protein hydrolysate product. These hydrolysate powders have been shown to be useful as animal feed and as a potential source of high quality nutrient for humans. Many types of enzymes have been employed successfully including a mixture of endo and exo enzymes derived from bacterial, fungal and plant sources as described in a recent review article, Shane M. Rutherford, "Methodology for Determining Degree of Hydrolysis of Proteins in Hydrolysates: A Review", Journal of AO AC International (2010) Volume: 93, Issue: 5, Page(s): 1515- 1522
- Aqua-culture and industrial fishing produces a large proportion of the protein consumed directly by humans as well as indirectly used in the form of fish meal fed to cattle, swine, poultry and fish-farms.
- Fish used for human consumption is increasingly sold as filleted products leaving a large portion of the fish protein as a waste byproducts.
- This industrial fish and fish off-cuts have been shown to be converted in multiple prior art instances into potentially useful soluble protein hydrolysate powder by enzyme hydrolysis as described in xx for example.
- a process typically described in the art may involve an enzymatic digestion, followed by a separation of the aqueous solution of amino acids and peptides i from the insoluble protein and bones and oil layer using commonly available decanters or filters.
- the protein powder produced from the soluble marine protein hydrolysate typically has a very high level of heavy metal contaminants such as mercury, lead, tin and arsenic which are environmental contaminants found in many marine zones.
- heavy metal contaminants such as mercury, lead, tin and arsenic which are environmental contaminants found in many marine zones.
- These heavy metals are extremely detrimental to human health even in parts per million concentrations as shown in Storeili, M.M., "Potential human health risks from metals (Hg, Cd, and Pb) and polychlorinated biphenyls (PCBs) via seafood consumption: Estimation of target hazard quotients (THQs) and toxic equivalents (TEQs)", Food and Chemical Toxicology, Volume 46, Issue 8, August 2008, Pages 2782-2788 and Roser Marti-Cida, Ana Bocioa, Juan M.
- THQs target hazard quotients
- TEQs toxic equivalents
- the present inventors have found that no commercially viable method has been described in the prior art for the removal of heavy metal contaminants from marine protein hydrolysates. Further surprisingly, the inventors have found that more than 99% of the heavy metals in the soluble fish protein hydrolysate are occluded in micron sized fatty particulates in the aqueous protein hydrolysate layer and removal of these fatty (lipid) particulates by ultra-filtration methods prior to evaporation and drying renders the finished protein powder significantly free of heavy metals at parts per billion concentrations thus rendering the protein powder much safer for human and animal consumption. Summary of the invention
- the present invention provides a new method to produce marine protein with ultra-low heavy metal contamination. More specifically, this invention relates to a commonly described enzyme hydrolysis of industrial fish or fish byproduct such as described in Suthasinee Nilsang, Sitiiwat Lertsiri, Manop Suphantharika and Apinya Assavanig., "Optimization of enzymatic hydrolysis of fish soluble concentrate by commercial proteases", Journal of Food Engineering, V olume 70, Issue 4, October 2005, Pages 571 - 578 but which additionally and noveily adds a separation step that comprises occluding the heavy metal contaminants present in the aqueous protein solution into a lipid particle and separating these lipid particles from the aqueous protein layer.
- This invention also relates to a method for separating these lipid particulates from the aqueous layer by using a continuously operating automa tic disc scrapper or membrane filtering system.
- the marine protein hydrolysate produced according to the present invention possess a ultra-low level of heavy metal contamination which makes it particularly suited for direct or indirect human consumption.
- the marine protein hydrolysate produced according to the present invention may optionally be used singly or mixed with other protein, lipid or calcium sources for use in animal and human nutrition.
- the method according to the present invention begins with the preparation of industrial fish or fish byproduct into a size and form most suitable for rapid enzyme hydrolysis via a process of grinding or mashing.
- Fish byproduct typically consists of the head and backbones of fish after the filleting process.
- the first step of the present invention is directed to provide a suitable particle size and composition for carrying out enzymatic hydrolysis using a variety of commercially available enzymes.
- protein hydrolysis can be effected by hundreds of known protease enzymes that can cleave an amide bond in the middle of a protein, such enzymes being commonly known as endo enzymes or cleave a protein from it's terminus one amino acid at a time, such enzymes being commonly known as exo enzymes.
- Common protease enzymes are commercially available and may be isolated from bacteria, fungi and plants.
- a preferred mix of endo and exo enzymes used in this invention ranges from a ratio of 2:8 to 8:2 depending on the source and nature of the industrial fish or fish byproduct to be hydrolyzed and the size, concentration and organoleptic properties desired in the marine protein hydrolysate powder produced.
- the result of the enzyme hydrolysis of industrial fish or fish off-cuts as carried out in this invention is a reaction mixture consisting of three phases (i) an aqueous phase consisting of soluble amino acids and peptides (ii) a oily phase consisting of a mixture of saturated and unsaturated fatty acids and phospholipids and (iii) an insoluble fraction consisting of small insoluble proteins and bone fragments.
- a preferred embodiment of the process of this invention is to separate the three phases from the enzymatic hydrolysis using a commercially available three phase decanter under a predominantly inert gas environment.
- a commercially available three phase decanter under a predominantly inert gas environment.
- Such an apparatus can be employed in batch or continuous manner to arrive at the three phases separated into individual receivers and the inert gas can be nitrogen.
- the aqueous layer of soluble marine protein hydrolysate is further subjected to filtration in a continuously operating automatic disc scrapper or membrane filtering system to enable the removal of 2 to 100 micron size lipid particulates in an industrially feasible manner. It should be noted that the lack of any filtration results in a marine protein hydrolysate powder with a total heavy metal concentration as measured for mercury, arsenic, lead and tin of approximately 100 ppm.
- a further preferred embodiment of this invention is to purify the oil layer by centrifugation as is commonly known to one skilled in the art and to render the insoluble protein and bone fraction into a dry powder without decomposition of the protein using any one of the drying techniques known to one skilled in the art such as a rotary press dryer or vacuum hot air dryer.
- the three fractions after enzyme hydrolysis, the aqueous soluble protein fraction, the oil fraction and the insoluble protein and bone fractions of this invention can be finally prepared as cosmetically, nutritionally and/or pharmaceutically acceptable forms which are suitable for each such application.
- 250 kg of head and back bone of salmon rendered after the filleting process are added into a grinder and processed into 10cm (long axis) irregular pieces.
- the ground material is transferred to a hydro lyzing reactor containing 250 liters of water and die stirred mixture is heated to 60C, 50 grams of commercially available exo and endo protease enzymes isolated from yeast are added into the hydro lyzing reactor in a 1 : 1 ratio and the mixture stirred for 1 hour.
- the enzymes are rendered inactive by heating the reaction to 90C for 15 minutes and the entire contents of the reactor are fed into a three phase decanter to effect the separation of three fractions while replacing the air with nitrogen at 1-2 atm pressure.
- the aqueous fraction is further passed through a continuously operating automatic disc scrapper or membrane filtering system and fed into a three phase evaporator to concentrate the dry matter concentration in the aqueous layer from 6% to 40% and the viscous liquid output from the evaporator is spray dried at 140C to give a dry powder with 92%+ dry matter.
- Example 2 Preparation of a marine protein hvdrolvsate without Alteration through a continuously operating automatic disc scrapper or membrane filtering system.
- Example 1 The process described in Example 1 was carried out exactly in the same manner except that the aqueous layer was not subjected to filtration in the continuously operating automatic disc scrapper or membrane filtering system prior to evaporation and spray drying.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Fats And Perfumes (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
L'invention concerne un procédé perfectionné pour produire une poudre d'hydrolysat de protéine marine soluble à contamination en métaux lourds ultra-faible. Cette invention concerne un procédé de production qui consiste à bloquer les contaminants de métaux lourds présents dans une solution d'hydrolysat de protéine marine soluble produite par une hydrolyse enzymatique de poisson industriel ou de chutes de poisson, dans une structure particulaire lipidique et à séparer ces particules de la couche de protéine aqueuse. Cette invention concerne en outre un procédé de séparation des particules grasses à partir de la couche aqueuse d'hydrolysat de protéine marine soluble consistant à utiliser un grattoir à disque automatique fonctionnant en continu ou un système de filtration sur membrane et à évaporer et à sécher la fraction aqueuse en une poudre d'hydrolysat de protéine meilleure pour la santé et plus sûre pour une consommation humaine directe et indirecte.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41995710P | 2010-12-06 | 2010-12-06 | |
| US81419957 | 2010-12-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2012078223A1 true WO2012078223A1 (fr) | 2012-06-14 |
Family
ID=46207441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2011/051315 WO2012078223A1 (fr) | 2010-12-06 | 2011-09-13 | Nouveau procédé pour produire un hydrolysat de protéine marine à contamination en métaux lourds ultra-faible |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2012078223A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014207497A1 (fr) * | 2013-06-29 | 2014-12-31 | Hofseth Biocare Asa | Nouveau procédé de production d'hydrolysat de protéines d'origine marine à contamination ultra-faible en métaux lourds et des propriétés physiques améliorées |
| CN109123069A (zh) * | 2018-08-14 | 2019-01-04 | 华南理工大学 | 一种高效去深海鱼中汞离子制备深海鱼蛋白水解物的方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5935605A (en) * | 1994-04-21 | 1999-08-10 | Stoilov; Ivan Lubomirov | Oral preparation for patients with chronic renal insufficiency, method of making and use |
| WO2001028353A2 (fr) * | 1999-10-20 | 2001-04-26 | Nordur Ehf | Hydrolysats proteiques obtenus a partir de proteases marines |
| US20030054084A1 (en) * | 2000-04-12 | 2003-03-20 | Hruschka Steffen M. | Method for the fractionation of oil and polar lipid-containing native raw materials |
| WO2005115176A1 (fr) * | 2004-05-26 | 2005-12-08 | Norcape Biotechnology As | Produit proteinique marin hydrolyse, son procede de production et son application |
| US20060099305A1 (en) * | 2004-05-17 | 2006-05-11 | Lee Chong M | Bioproduction of hydrolysate from squid processing byproducts for aquaculture feed ingredient and organic fertilizer |
| US20070142274A1 (en) * | 2003-07-04 | 2007-06-21 | Rolf Berge | Fish protein hydrolyzate |
-
2011
- 2011-09-13 WO PCT/US2011/051315 patent/WO2012078223A1/fr active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5935605A (en) * | 1994-04-21 | 1999-08-10 | Stoilov; Ivan Lubomirov | Oral preparation for patients with chronic renal insufficiency, method of making and use |
| WO2001028353A2 (fr) * | 1999-10-20 | 2001-04-26 | Nordur Ehf | Hydrolysats proteiques obtenus a partir de proteases marines |
| US20030054084A1 (en) * | 2000-04-12 | 2003-03-20 | Hruschka Steffen M. | Method for the fractionation of oil and polar lipid-containing native raw materials |
| US20070142274A1 (en) * | 2003-07-04 | 2007-06-21 | Rolf Berge | Fish protein hydrolyzate |
| US20060099305A1 (en) * | 2004-05-17 | 2006-05-11 | Lee Chong M | Bioproduction of hydrolysate from squid processing byproducts for aquaculture feed ingredient and organic fertilizer |
| WO2005115176A1 (fr) * | 2004-05-26 | 2005-12-08 | Norcape Biotechnology As | Produit proteinique marin hydrolyse, son procede de production et son application |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014207497A1 (fr) * | 2013-06-29 | 2014-12-31 | Hofseth Biocare Asa | Nouveau procédé de production d'hydrolysat de protéines d'origine marine à contamination ultra-faible en métaux lourds et des propriétés physiques améliorées |
| CN109123069A (zh) * | 2018-08-14 | 2019-01-04 | 华南理工大学 | 一种高效去深海鱼中汞离子制备深海鱼蛋白水解物的方法 |
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