WO2012053035A1 - Matériau d'implant, procédé de production de ce matériau et méthode destinée à améliorer la compatibilité avec les ostéocytes - Google Patents

Matériau d'implant, procédé de production de ce matériau et méthode destinée à améliorer la compatibilité avec les ostéocytes Download PDF

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WO2012053035A1
WO2012053035A1 PCT/JP2010/006247 JP2010006247W WO2012053035A1 WO 2012053035 A1 WO2012053035 A1 WO 2012053035A1 JP 2010006247 W JP2010006247 W JP 2010006247W WO 2012053035 A1 WO2012053035 A1 WO 2012053035A1
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functional group
group containing
abundance ratio
less
carbonaceous film
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PCT/JP2010/006247
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English (en)
Japanese (ja)
Inventor
牧平清超
二川浩樹
峯裕一
岡本圭司
阿部義紀
中谷達行
新田祐樹
Original Assignee
国立大学法人広島大学
トーヨーエイテック株式会社
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Priority to PCT/JP2010/006247 priority Critical patent/WO2012053035A1/fr
Publication of WO2012053035A1 publication Critical patent/WO2012053035A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
    • A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
    • A61C8/0012—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
    • A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
    • A61C8/0012—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy
    • A61C8/0013—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy with a surface layer, coating
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/04—Metals or alloys
    • A61L27/06—Titanium or titanium alloys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/30—Inorganic materials
    • A61L27/303—Carbon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/30767—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
    • A61F2002/3093—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth for promoting ingrowth of bone tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment

Definitions

  • the present disclosure relates to an implant material, a method for producing the same, and a method for improving affinity with bone cells, and more particularly, to a material that requires affinity for bone cells such as artificial tooth roots and dentures and a method for producing the same.
  • titanium and titanium alloys are used as a base material for implants embedded in living bodies such as artificial tooth roots because they are excellent in biocompatibility, corrosion resistance and mechanical strength.
  • an artificial dental root made of titanium or titanium alloy is directly fixed to the jawbone, the metabolic balance of bone tissue regeneration of the jawbone may be lost, and the artificial root may loosen or the jawbone may be destroyed.
  • peri-implantitis As a cause of the failure of such artificial tooth root implantation, there is peri-implantitis in which osteoclasts are induced around the artificial tooth root and bone destruction occurs. Peri-implantitis may result from microbial stimulation such as bacterial infection and mechanical stimulation such as excessive bite force, but also occurs due to osteoclast activation by the artificial tooth root itself.
  • osteoclasts Activation of osteoclasts by artificial tooth roots also occurs at the initial implantation of the implant. Osteoclasts that appear at the time of initial implantation interfere with the osseointegration of the implant and cause the implant to fail.
  • osteoclasts Such activation of osteoclasts is thought to be caused by the fact that titanium and titanium alloys do not have sufficient affinity with bone cells. On the surface of a material having insufficient affinity for bone cells, differentiation from osteoclast precursor cells to osteoclasts is promoted, thereby causing bone destruction. Titanium and titanium alloys are known to have relatively high affinity for bone cells. However, it cannot be said that the affinity is sufficient, and the implantation of the artificial tooth root fails due to the condition of the jaw bone of the person to be implanted, the condition in the oral cavity, and the like.
  • DLC film diamond-like thin film
  • the DLC film has carbon as a main component and has a smooth and inert surface, so that it is excellent in compatibility with a living body. For this reason, it is thought that it is excellent also in the affinity with a bone cell.
  • the affinity between the implant and the bone cells needs to be examined not only from the suppression of osteoclast induction but also from the viewpoint of promoting differentiation into osteoblasts. Even if the differentiation from osteoclast precursor cells to osteoclasts can be suppressed, if the differentiation from immature osteoblasts to osteoblasts is suppressed or the proliferation of osteoblasts is inhibited, the osseointegration of the implant Is not promoted. To date, little is known about how the DLC membrane affects osteoblast differentiation.
  • the present disclosure is based on the knowledge of the effect of the DLC membrane on the differentiation into osteoblasts obtained by the inventors of the present application, and suppresses the induction of osteoclasts and promotes the differentiation into osteoblasts.
  • An object of the present invention is to realize a material for implants.
  • the implant material according to the present disclosure includes a base material and a carbonaceous thin film formed on the surface of the base material, and the carbonaceous thin film includes at least a functional group containing oxygen and a functional group containing nitrogen.
  • the ratio of the functional group containing oxygen is 4% or less, and the value obtained by dividing the abundance ratio of the functional group containing nitrogen by the abundance ratio of the functional group containing oxygen is 10 or less.
  • the implant material of the present disclosure can suppress differentiation into osteoclasts and promote differentiation into osteoblasts, thus realizing an implant material with excellent affinity with bone tissue it can.
  • the base material may be a metal.
  • a base material consists of titanium or a titanium alloy.
  • the base material may be an artificial tooth root, a denture, a crown restoration, an artificial bone, or an artificial joint.
  • the zeta potential on the surface of the carbonaceous film may be ⁇ 50 mV or more and less than 0 mV.
  • the method for producing an implant material according to the present disclosure includes a step (a) of preparing a base material, and a step of forming a carbonaceous film on the surface of the base material by a chemical vapor deposition method using a gas containing a hydrocarbon. (B) and a step (c) for maintaining the substrate in a vacuum state until the dangling bonds on the surface of the carbonaceous film formed on the surface of the substrate are stabilized, and a function including oxygen in the carbonaceous film
  • the abundance ratio of the group is 4% or less, and the value obtained by dividing the abundance ratio of the functional group containing nitrogen by the abundance ratio of the functional group containing oxygen is 10 or less.
  • the method for producing an implant material according to the present disclosure may further include a step (d) of irradiating the carbonaceous film with plasma of a basic nitrogen-containing compound after the step (c).
  • a carbonaceous film having at least one of a functional group containing oxygen and a functional group containing nitrogen is formed on the surface of a base material,
  • the abundance ratio of the group is 4% or less, and a value obtained by dividing the abundance ratio of the functional group containing nitrogen by the abundance ratio of the functional group containing oxygen is 10 or less.
  • an implant material that suppresses induction into osteoclasts and promotes differentiation into osteoblasts can be realized.
  • FIG. 6 is a cross-sectional view illustrating an exemplary implant material. It is a graph which shows the relationship between the surface state of a base material, and the proliferation of an osteoblast. It is a graph which shows the relationship between the surface state of a base material, and the differentiation to an osteoblast. It is a graph which shows the relationship between the surface state of a base material, and the differentiation to an osteoblast. It is a graph which shows the relationship between the surface state of a base material, and the differentiation to an osteoclast.
  • the implant includes not only an artificial tooth root but also a denture, a crown restoration material, a denture restoration material, and the like.
  • it also includes instruments that need to be compatible with bone cells, such as artificial bones and artificial joints, that are implanted in a living body.
  • the carbonaceous film is a film containing sp 2 carbon-carbon bonds (graphite bonds) and sp 3 carbon-carbon bonds (diamond bonds) represented by diamond-like films (DLC films). It may be an amorphous film such as a DLC film or a crystalline film such as a diamond film. Typically, it contains sp 2 carbon-hydrogen bonds and sp 3 carbon-hydrogen bonds, but hydrogen is not an essential component. Further, silicon (Si), fluorine (F), or the like may be added.
  • FIG. 1 shows a cross-sectional configuration of the implant material according to the present embodiment.
  • a carbonaceous film 20 having a film thickness of about 0.005 ⁇ m to 3 ⁇ m is formed on the surface of the substrate 10.
  • At least one of a functional group containing oxygen typified by a carboxyl group and a functional group containing nitrogen typified by an amino group is present on the surface of the carbonaceous film 20.
  • the abundance ratio of the functional group containing oxygen in the carbonaceous film 20 is 4% or less. Further, the value obtained by dividing the abundance ratio of the functional group containing nitrogen by the abundance ratio of the functional group containing oxygen is 10 or less.
  • the abundance ratio of the functional group containing oxygen refers to the peak area of the 1s (O1s) peak of oxygen obtained by X-ray photoelectron spectroscopy as the peak area of the 1s (C1s) peak of carbon.
  • the abundance ratio of the functional group containing nitrogen is a value obtained by dividing the peak area of the 1s (N1s) peak of nitrogen by the peak area of the 1s (C1s) peak of carbon.
  • Affinity with bone cells refers to both the promotion of differentiation from immature osteoblasts to osteoblasts, the promotion of osteoblast proliferation, and the suppression of induction from osteoclast precursor cells to osteoclasts. It is necessary to evaluate from.
  • a carbonaceous film is formed on the surface of the substrate, and the abundance ratio of functional groups including oxygen such as carboxyl groups on the surface of the carbonaceous film is 4% or less, and the abundance ratio of functional groups including nitrogen such as amino groups is carboxyl.
  • platelets have a great influence on treatment results.
  • treatment is more reliably performed by immersing the implant in plasma containing platelets (Platelet-Rich Plasma) obtained from the patient's blood before the implant is placed, and adsorbing the platelet to the surface of the implant. It has been.
  • the abundance ratio of amino group and carboxyl group on the surface of the carbonaceous film affects the zeta potential on the surface of the carbonaceous film, and the value of the zeta potential zeta potential on the surface of the carbonaceous film is ⁇ 50 mV or less. In this case, it is known that platelets hardly adsorb.
  • the abundance ratio of the carboxyl group which is a functional group containing oxygen is 4% or less and the abundance ratio of the amino group which is a functional group containing nitrogen / the abundance ratio of the carboxyl group which is a functional group containing oxygen is 10 or less
  • the value of the potential is about ⁇ 60 mV to about 0 mV.
  • the zeta potential on the surface of the carbonaceous film is preferably ⁇ 50 mV or more and 0 mV or less.
  • the implant material of the present embodiment covers the surface of the base material with a carbonaceous film, and therefore exhibits an excellent affinity for bone tissue regardless of the material of the base material.
  • the base material may be any material as long as it satisfies characteristics such as strength.
  • metals such as titanium and titanium alloys, resins, ceramics, and the like can be used.
  • it can be applied to various implants that require affinity for bone cells such as artificial tooth roots, dentures, artificial bones, and artificial joints.
  • the carbonaceous film may be formed by a chemical vapor deposition method (CVD method). Further, it may be formed by sputtering, plasma ion implantation, ion plating, arc ion plating, ion beam vapor deposition, laser ablation, or the like other than CVD.
  • CVD method chemical vapor deposition method
  • the purity of the argon gas used for plasma generation is set to 99.9999% or more, and after the film formation is finished, the substrate temperature becomes room temperature, preferably at least 10 minutes. May be left in a vacuum for 60 minutes or longer.
  • the carbonaceous film may be irradiated with plasma of a basic nitrogen-containing compound.
  • basic nitrogen-containing compounds include ammonia and organic amines represented by the general formula NR 1 R 2 R 3 (where R 1 , R 2 and R 3 are hydrogen, —CH 3 , —C 2 H 5 , —C 3 H 7 or —C 4 H 8 , and R 1 , R 2 and R 3 may be the same or different from each other.) Or benzylamine and its secondary and tertiary amines Etc. may be used.
  • ammonia is preferable because of cost and ease of handling.
  • the ultimate vacuum in the chamber during plasma irradiation may be about 0.01 Pa to about 500 Pa. However, it is preferable that the ultimate vacuum is higher without being affected by oxygen in the air, and may be about 5 ⁇ 10 ⁇ 3 Pa.
  • oxygen plasma or plasma of gas containing oxygen may be irradiated. Further, the ultimate vacuum at the time of irradiation with plasma such as ammonia may be lowered.
  • a plasma irradiation apparatus having any structure may be used.
  • any type of discharge may be used.
  • Plasma irradiation conditions are not particularly limited.
  • the power source for generating plasma various power source frequencies such as a commercial frequency (50 Hz or 60 Hz), a high frequency (radio frequency), or a microwave region can be used.
  • the pressure control method and supply structure of the source gas are not particularly limited. However, if plasma irradiation conditions with a very high etching rate are used, the carbonaceous thin film may be damaged.
  • the thickness of the carbonaceous film is not particularly limited, but is preferably in the range of 0.005 ⁇ m to 3 ⁇ m, more preferably in the range of 0.01 ⁇ m to 1 ⁇ m.
  • the carbonaceous film can be directly formed on the surface of the base material, but an intermediate layer is provided between the base material and the carbonaceous thin film in order to more firmly adhere the base material and the carbonaceous thin film. Also good.
  • the material for the intermediate layer various materials can be used depending on the type of substrate, but silicon (Si) and carbon (C), titanium (Ti) and carbon (C), or chromium (Cr) and carbon.
  • a known film such as an amorphous film made of (C) can be used.
  • the thickness is not particularly limited, but is preferably in the range of 0.005 ⁇ m to 0.3 ⁇ m, and more preferably in the range of 0.01 ⁇ m to 0.1 ⁇ m.
  • the intermediate layer may be formed using, for example, a sputtering method, a CVD method, a plasma CVD method, a thermal spraying method, an ion plating method, an arc ion plating method, or the like.
  • a carbonaceous film made of a DLC film was formed on the surface of a base material made of pure titanium (JIS type 2).
  • a substrate with a diameter of 20 mm was used for cell culture.
  • the DLC film was formed using a chemical vapor deposition (CVD) method. Specifically, C 2 H 2 was introduced into the chamber on which the substrate was placed so that the flow rate was 150 sccm (cm 3 / min, but 1 atm, 0 ° C.) and the pressure was 24.67 Pa (35 mTorr).
  • a high frequency power of 100 W to 500 W was applied to the RF electrode.
  • the DLC film having a low abundance ratio of oxygen-containing functional groups can be obtained by leaving it in a vacuum.
  • Table 1 shows the abundance ratio of the functional group containing oxygen and the abundance ratio of the functional group containing nitrogen when the DLC film is left in a vacuum for 10 minutes after being formed and in a vacuum for 120 minutes.
  • the functional group containing oxygen is regarded as a carboxyl group (COOH), and the functional group containing nitrogen is regarded as an amino group (NH 2 ) for evaluation.
  • the abundance ratio of the functional group containing oxygen can be reduced to 4% or less by leaving it in a vacuum for 10 minutes or more after film formation. In addition, the longer the time of leaving in vacuum, the smaller the abundance ratio of the functional group containing oxygen.
  • Plasma irradiation was performed to further introduce functional groups into the carbonaceous film.
  • Plasma irradiation was performed by a parallel plate type plasma irradiation apparatus. After setting the base material obtained above in the chamber of the plasma irradiation apparatus, the pressure in the chamber was evacuated to 2 Pa or less. Next, ammonia or oxygen was introduced into the chamber at a predetermined flow rate, and plasma was generated by applying high frequency power of 30 W between the parallel plate electrodes. The gas flow rate was adjusted by a mass flow controller, and the pressure in the chamber during plasma irradiation was 130 Pa. The high frequency power was applied using a high frequency power source connected via a matching box. Ammonia was used to introduce a functional group containing nitrogen, and oxygen was used to introduce a functional group containing oxygen. The plasma irradiation time was 15 seconds.
  • the abundance ratio of functional groups in the carbonaceous film was evaluated by X-ray photoelectron spectroscopy (XPS) measurement.
  • XPS X-ray photoelectron spectroscopy
  • An aluminum K ⁇ ray was used as the X-ray source, the acceleration voltage was 10.0 KV, and the emission current was 10 mA.
  • the incident angle of X-rays was 45 degrees, and the state from the surface to a depth of about 4 nm was measured.
  • the abundance ratio of the functional group containing nitrogen was the ratio of the area of the 1s (N1s) peak of nitrogen and the area of the carbon (C) 1s peak obtained in XPS measurement. It is not clear what state nitrogen is present on the surface of the carbonaceous film. However, it is considered that functional groups containing nitrogen (nitrogen functional groups) such as amino groups and amide groups are formed. Although detailed analysis of the nitrogenous functional group is difficult, it is considered that it exists mainly as an amino group because an N1s peak appears in the vicinity of 399 eV in XPS measurement.
  • the ratio of the area of the N1s peak to the area of the C1s peak will be described as the abundance ratio [NH 2 ] of the amino group.
  • the abundance ratio of the functional group containing oxygen was the ratio of the area of the 1s (O1s) peak of oxygen and the area of the C1s peak obtained in the XPS measurement.
  • a hydroxyl group other than a carboxyl group may be formed as a functional group containing oxygen, but in the following, the ratio of the area of the O1s peak to the area of the C1s peak is referred to as a carboxyl group abundance ratio [COOH]. .
  • the abundance ratio obtained from the ratio of peak areas is atomic% (at%).
  • MC3T3-E1 cell mouse osteoblast-like cell line MC3T3-E1 cell
  • MC3T3-E1 cell mouse osteoblast-like cell line
  • FBS fetal bovine serum
  • L-glutamine L-glutamine
  • mixed antibiotics Invitrogen
  • 50 ⁇ g / ml ascorbic acid was used as the culture medium.
  • the culture temperature was 37 ° C., and the culture was performed in a 5% carbon dioxide atmosphere.
  • the wavelength of absorbance measurement for evaluating proliferation was 490 nm.
  • the sample and osteoclast precursor cells were contacted in the presence of an osteoclast differentiation inducing factor (Receptor Activator of NF-kB Ligand: RANKL) and cultured at 37 ° C.
  • an osteoclast differentiation inducing factor Receptor Activator of NF-kB Ligand: RANKL
  • RANKL osteoclast differentiation inducing factor
  • As the osteoclast precursor cells cell line RAW264.7 cells (TIB-71, ATCC) that have been established to differentiate into osteoclasts in the presence of RANKL were used. Cells were seeded at 5 ⁇ 10 3 cells per well.
  • Sample 1 is a pure titanium base material on which a DLC film provided for control is not formed.
  • Sample 2 is a DLC film not subjected to plasma irradiation.
  • Sample 3 is a DLC film in which the amount of carboxyl groups introduced is increased by plasma irradiation.
  • Sample 4 is a DCL film in which the amount of amino groups introduced is increased by plasma irradiation.
  • FIG. 2 shows the growth characteristics of MC3T3-E1 cells.
  • the vertical axis indicates the absorbance at 490 nm, and the higher the absorbance, the more the MC3T3-E1 cells are proliferating.
  • the proliferation of MC3T3-E1 cells did not decrease even when a DLC film was formed.
  • the proliferation of MC3T3-E1 was not affected by the amount of functional groups introduced into the DLC membrane.
  • FIG. 3 and 4 show the differentiation characteristics of MC3T3-E1 cells.
  • the vertical axis indicates the mRNA expression level of Runx2, and the larger the value, the more the Runx2 is expressed, indicating that the differentiation of MC3T3-E1 cells is promoted.
  • the vertical axis represents the amount of CoI-I mRNA expression. The larger the value, the more CoI-I is expressed and the differentiation of MC3T3-E1 cells is promoted.
  • Sample 2 the expression levels of Runx2 and CoI-I increased about 200 times that of pure titanium. However, in sample 3 with [COOH] exceeding 4% and sample 4 with [NH 2 ] / [COOH] exceeding 10, no effect of promoting differentiation into osteoblasts was observed.
  • FIG. 5 shows the expression characteristics of cathepsin K, which is an index of differentiation into osteoclasts.
  • RANKL which is a differentiation promoting factor
  • the implant material and the manufacturing method thereof according to the present disclosure can realize an implant material that suppresses induction into osteoclasts and promotes differentiation into osteoblasts, and particularly bone cells such as artificial tooth roots and dentures. It is useful as a dental material that requires an affinity for and a manufacturing method thereof.

Abstract

L'invention concerne un matériau d'implant comprenant un substrat (10) et une couche mince carbonée (20) formée sur la surface du substrat (10). La couche mince carbonée (20) contient au moins des groupes fonctionnels contenant de l'oxygène ou des groupes fonctionnels contenant de l'azote. Le pourcentage de groupes fonctionnels contenant de l'oxygène est de 4% ou inférieur et le rapport de teneurs groupes fonctionnels contenant de l'azote-groupes fonctionnels contenant de l'oxygène n'est pas supérieur à 10.
PCT/JP2010/006247 2010-10-21 2010-10-21 Matériau d'implant, procédé de production de ce matériau et méthode destinée à améliorer la compatibilité avec les ostéocytes WO2012053035A1 (fr)

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PCT/JP2010/006247 WO2012053035A1 (fr) 2010-10-21 2010-10-21 Matériau d'implant, procédé de production de ce matériau et méthode destinée à améliorer la compatibilité avec les ostéocytes

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PCT/JP2010/006247 WO2012053035A1 (fr) 2010-10-21 2010-10-21 Matériau d'implant, procédé de production de ce matériau et méthode destinée à améliorer la compatibilité avec les ostéocytes

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009137888A (ja) * 2007-12-06 2009-06-25 Hiroshima Univ インプラント用材料及びその製造方法
WO2010092616A1 (fr) * 2009-02-10 2010-08-19 国立大学法人広島大学 Matériau d'implant et son procédé de production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009137888A (ja) * 2007-12-06 2009-06-25 Hiroshima Univ インプラント用材料及びその製造方法
WO2010092616A1 (fr) * 2009-02-10 2010-08-19 国立大学法人広島大学 Matériau d'implant et son procédé de production

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KUMARI T.V. ET AL.: "In vitro cytocompatibility studies of Diamond Like Carbon coatings on titanium.", BIO-MEDICAL MATERIALS AND ENGINEERING, vol. 12, no. 4, 2002, pages 329 - 338 *
OKAMOTO K. ET AL.: "Differentiation Capability Evaluation of Osteoblast by Functionalized DLC Thin Films with Plasma Processing", JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, vol. 23, no. 4, 6 July 2010 (2010-07-06), pages 591 - 594 *
SATOSHI IWATA ET AL.: "DLC Coating shita Titanium ga Kotsuga Saiboyo Saibokabu MC3T3-E1 Saibo no Bunka ni Ataeru Eikyo", JOURNAL OF JAPANESE SOCIETY OF ORAL IMPLANTOLOGY, vol. 22, 25 August 2009 (2009-08-25), pages 231, P-1-6 *
YASUHIRO OKADA ET AL.: "Kakushu DLC Kiban Jo deno Kotsugayo Saibo no Zoshoku to Bunka ni Tsuite", POLYMER PREPRINTS, JAPAN(CD-ROM), DISKL, vol. 59, no. 2, 1 September 2010 (2010-09-01), JAPAN, pages ROMBUNNO.1PC067 *

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