WO2012011130A2 - Composition enzymatique et procédé pour l'extraction d'huile à partir des fruits du palmier à huile - Google Patents

Composition enzymatique et procédé pour l'extraction d'huile à partir des fruits du palmier à huile Download PDF

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Publication number
WO2012011130A2
WO2012011130A2 PCT/IN2011/000491 IN2011000491W WO2012011130A2 WO 2012011130 A2 WO2012011130 A2 WO 2012011130A2 IN 2011000491 W IN2011000491 W IN 2011000491W WO 2012011130 A2 WO2012011130 A2 WO 2012011130A2
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Prior art keywords
oil
enzyme composition
atleast
mesocarp
activity
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PCT/IN2011/000491
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English (en)
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WO2012011130A8 (fr
WO2012011130A3 (fr
Inventor
Chandrakant Laxminarayan Rathi
Saylee Pradhan
Sambasivarao Javvadi
Ahila Wani
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Advnaced Enzyme Technologies Ltd.
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Publication of WO2012011130A2 publication Critical patent/WO2012011130A2/fr
Publication of WO2012011130A3 publication Critical patent/WO2012011130A3/fr
Publication of WO2012011130A8 publication Critical patent/WO2012011130A8/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases

Definitions

  • TITLE AN ENZYME COMPOSITION AND PROCESS FOR EXTRACTING OIL FROM OIL PALM FRUITS
  • the present invention relates to an enzyme composition and enzymatic process for extracting oil from oil bearing fruits. More particularly, the present invention relates to an enzyme composition and process for extracting oil from oil palm fruits.
  • Crude Palm oil is edible plant oil extracted from the pulp that is fleshy mesocarp of the fruit of the oil palm Elaeis guineensis. Mescocarp accounts fro 60% of the total composition of the oil palm fruit.
  • the oil derived from the mesocarp has been long recognized as cooking ingredient and is widely used as cooking oil in West African countries and Southeast Asia. Its use has increased in the commercial food industry owing to its high oxidative stability when used for frying. It has also been used in cosmetics.
  • Crude Palm oil is composed of fatty acids. It is high in 16-carbon saturated fatty acid palmitic acid; another major component of crude palm oil is monounsaturated oleic acid and it is low in myristic acid with virtually no lauric acid. Such composition makes crude palm oil healthier cooking oil compared to palm kernel oil which is derived from the same plant but from seeds. Both the oils are very different in chemical composition as well as physical properties. Palm kernel oil chiefly comprises of lauric acid and myristic acid and less of palmitic acid and stearic acid. Studies have shown that both stearic acid and palmitic acid, which comprise virtually all the saturated fats in palm oil, have neutral to favorable impact on serum lipid profiles compared to lauric and myristic acid.
  • Crude or Red palm oil not only comprises healthy composition of fatty acids, but also contains a blend of vitamins, antioxidants, and other phytonutrients important for good health.
  • Red palm oil is the richest dietary source of provitamin A carotenes (beta- carotene and alpha-carotene).
  • provitamin A carotenes beta- carotene and alpha-carotene.
  • beta-carotene, alpha-carotene it contains lycopene and at least 10 other carotenes, along with tocopherols and tocotrienols (members of the vitamin E family), CoQ.10, phytosterols, and glycolipids.
  • palm oil is blended into mayonnaise and salad oil, fortified into foods for specific health use and anti-aging cosmetics.
  • the palm oil is extracted from the mesocarp of the palm fruits by employing very complex and harsh mechanical process.
  • the commercial palm oil extraction consists of pulping and pressing of fruits to get oil and juice from solid material.
  • Fruits are subjected to pressurized steam for sterilization, they are then cooked by using hot water and high heat, after which they are digested in digester commonly consisting of steam-heated cylindrical vessel fitted with a central rotating shaft carrying a number of beater (stirring) arms, to rupture and break down oil bearing cells, and finally oil is extracted by wet or dry methods.
  • digester commonly consisting of steam-heated cylindrical vessel fitted with a central rotating shaft carrying a number of beater (stirring) arms, to rupture and break down oil bearing cells, and finally oil is extracted by wet or dry methods.
  • the oil is squeezed out of a mixture of oil, moisture, fibre and nuts by applying mechanical pressure on the digested mash.
  • the fluid coming out of the press is a mixture of palm oil, water, cell debris, fibrous material and 'non-oily solids'. Because of the non-oily solids the mixture is very thick (viscous).
  • hot water is added to the press output mixture to thin it.
  • the diluted mixture is passed through a screen to remove coarse fibre.
  • the screened mixture is boiled from one or two hours and then allowed to settle by gravity in the large tank so that the palm oil, being lighter than water can separate and rise to the top.
  • the moisture content of the oil has to be reduced to 0.15 to 0.25 percent.
  • the oil palm mesocarp contains high percentage of gums in addition to oil and water which remains attached to the fibers and makes it difficult to separate gums from fibers and liberate oil.
  • very high temperature and harsh mechanical treatment as much as 30% or even more oil remains entrapped into fibrous, very viscous and sticky mass reducing oil extraction efficiency considerably.
  • many of the oil constituents get degraded resulting into inferior oil, further owing to large quantity of water used during the extraction, which if not removed sufficiently can increase the free fatty acid content, further deteriorating oil quality.
  • WO/2007/038963 discloses a process and device for producing palm oil or vegetable edible oil from fresh fruits. It contain the steps of extracting crude oil from the fruits, clarifying the crude oil in a two-phase decanter and divide it into sludge and oil, and mechanically separating sludge water from the sludge of the two-phase clarification.
  • the present invention provides an enzyme composition comprising enzymes having exocellulolytic, pectinolytic, mannanolytic and glucanolytic activity for extracting oil from oil palm fruits with improved efficiency.
  • the present invention provides an enzyme composition comprising atleast one of exocellulolases, atleast one of pectinases, atleast one of mannanases and atleast one of glucanases for extracting oil from oil palm fruits, particularly from mesocarp portion or mesocarp containing oil palm fruits with atleast 90% oil yield.
  • the present invention provides an enzyme composition comprising exocellulase having atleast 5000 u/gm activity, pectinase having atleast 500 u/gm activity, mannanase having atleast 10000 u/gm activity and glucanase having atleast 500 u/gm activity for extracting oil from oil palm fruits, particularly from mesocarp portion or mesocarp containing oil palm fruits with atleast 95% oil yield.
  • the present invention provides use of an enzyme composition of the present invention for extracting oil from oil palm fruits, particularly from mesocarp portion or mesocarp containing oil palm fruits with improved yield.
  • the present invention provides an enzymatic process for extracting oil from oil palm fruits with improved efficiency and/or increased yield comprising steps of: incubating the mixture containing mashed mesocarp portion or mesocarp containing mashed oil palm fruit mass, water and enzyme composition of the present ⁇ invention at about 25-65 degree Celsius for atleast 60 -.120 minutes and separating the palm oil from the enzyme treated mass and water.
  • the present invention is directed to an enzyme composition and a process for extracting oil from oil palm that is Elaeis guineensis fruits with improved efficiency.
  • the present invention is specifically directed to an enzyme composition and a process for extracting oil from mesocarp portion or mesocarp containing oil palm fruits with improved efficiency.
  • the mesocarp portion is the outer fleshy, fibrous part of oil palm fruit surrounding the kernel or seed.
  • the "mesocarp containing oil palm fruits" as used in the present invention refers to the entire oil palm fruits essentially containing mesocarp portion as well as other part of the fruit that is kernel.
  • an enzyme composition comprises enzymes capable of extracting oil from from mesocarp portion or mesocarp containing oil palm fruits with improved efficiency and/or increased yield of atleast 90%, more preferably with atleast 95% yield.
  • the enzymes composition of the present invention preferably comprises enzymes that extract oil from the mesocarp portion of the oil palm fruit but which essentially do not extract oil from the kernel or the seed part of the oil palm fruit.
  • the present invention provides an enzyme composition comprising enzymes having activities selected from exocellulolytic, pectinolytic, mannanolytic, and glucanolytic for extracting oil from oil palm fruits with improved efficiency.
  • the present invention provides an enzyme composition comprising atleast one of exocellulolases, atleast one of pectinases, atleast one of mannanases and atleast one of glucanases for extracting oil from oil palm fruits with atleast 90% oil recovery.
  • the enzyme composition of the present invention comprises enzymes in an effective amount so as to extract oil from oil palm fruit with improved efficiency and/or increased yield.
  • the present invention provides an enzyme composition comprising exocellulase having atleast 5000 u/gm activity, pectinase having atleast 500 u/gm activity, mannanase having atleast 10000 u/gm activity and glucanase having atleast 500 u/gm activity. It was surprisingly found that the composition of the present invention comprising these specified enzymes in specified quantities provided enhanced oil extraction efficiency with ability of extracting atleast 95% of oil, particularly from mesocarp portion of the oil palm fruit.
  • the enzyme composition comprises exocellulase having 5000 u/gm to 20000 u/gm activity.
  • the enzyme composition comprises pectinase having 500 u/gm to 5000 u/gm activity.
  • the enzyme composition comprises mannanase having 10000 u/gm to 35000 u/gm activity.
  • the enzyme composition comprises glucanase having 500 u/gm to 5000 u/gm activity.
  • enzymes may be added on the basis of percentage in the enzyme composition.
  • exocellulase may be included in the range of about 2.5% to 8.5%.
  • Pectinase may be included in the range of about 0.5% to 5%.
  • Mannanase may be included in the range of about 25% to 65%.
  • Glucanase may be included in the range of about 0.35% to 3.5%.
  • the enzyme composition of the present invention may optionally further comprise one or more additional enzyme having activity selected from amylolytic, arabinolytic, glucosidolytic, amyloglucosidolytic, endocellulolytic, ferulic acid esterolytic, hemicellulolytic, xylanolytic, proteolytic and/or phytolytic.
  • the enzyme composition of the present invention may be formulated in the dry powder form or liquid form.
  • the enzyme composition in powder form may be formulated by blending enzymes with inert fillers selected from the group consisting of but not limited to lactose, maltodextrin, starch, microcrystalline cellulose, sucrose and/or dextrin.
  • the enzyme composition of the present invention in liquid form may be formulated by dispersing enzymes in the base medium selected from group consisting of but not limited to glycerin, sorbitol and/or water.
  • the present invention provides use of an enzyme composition of the present invention for extracting oil from oil palm fruits, particularly from mesocarp portion or mesocarp containing oil palm fruit with improved efficiency, preferably atleast 90% recovery, more preferably with atleast 95% recovery of oil.
  • the present invention provides a process for extracting oil from 3 ⁇ 4 pil palm fruits with an increased yield, wherein process comprises steps of: incubating the mixture containing mashed mesocarp portion or mesocarp containing mashed oil palm fruit mass, water and enzyme composition of the present invention at about 25-65 degree Celsius for atleast 60 - 120 minutes and separating the palm oil from the enzyme treated mass and water.
  • the mashed mass of the oil palm fruit may be obtained by crushing the oil palm fruits with seeds and mesocarp in suitable mashing equipment for a sufficient time and preferably at a higher temperature to get the mashed mass of oil palm fruits with seeds remaining intact.
  • Mashing equipments to obtain the mashed mass of oil palm fruit should be such which mashes the fibrous part essentially comprising of mesocarp but keeps the seed intact, such mashing equipment may be selected from mixer, spiral mixer and or shredder or stripper or other suitable equipment.
  • Mashing time of oil palm fruit may depend upon the mashing equipment used, for example when spiral mixer is used mashing may be carried out for 10 minutes to 30 minutes.
  • oil palm fruits Prior to mashing oil palm fruits are steamed and /or sterilized by subjecting them to suitable steaming and or sterilizing method.
  • oil palm fruits are steamed at 130 degrees Celsius to 150 degree Celsius temperature and under high pressure for example 2.5 to 3.5kg pressure.
  • the steamed and mashed mesocarp portion or oil palm fruit mass is mixed with water and the enzyme composition as per the present invention by any suitable homogenizing means for example by stirring.
  • the mixture containing mashed mashed oil palm fruit mass and water is obtained by adding water in a specific ratio to substrate that is mesocarp portion or mesocarp containing oil palm fruit.
  • the ratio of substrate to water - is from about 1: 0.5 to about 1:2.5, preferably in the ratio of 1:0.75 to 1:1.75.
  • the enzyme composition of the present invention may be added in a range from about 0.1 % to about 5% on w/w basis.
  • the enzyme composition is added in an amount from about 0.5 % to about 3.5% on w/w basis.
  • the mixture comprising the mashed oil palm fruit mass, enzyme composition and water is incubated for sufficient time and under suitable conditions allowing enzyme composition to act on the crushed oil palm fruit mass and provide the oil with higher yield.
  • the mixture of crushed oil palm fruit mass, enzyme and water is incubated for about 1 hour to about 24 hours, preferably from about 2 hrs to about 6 hrs.
  • the mixture is incubated at the temperature of about 25 - 70 degree Celsius, preferably from 45 - 65 degree Celsius.
  • the enzyme treated mixture thus obtained has the liquid consistency so as to provide much ease in further processing as against the commercial methods.
  • the oil palm fruit mass after digestion is highly thick viscous mass having gums adhering to the fibers thereby making the extraction of oil therefrom very difficult.
  • the conventional method of dry extraction the oil extracted from such mass is incomplete and does not give good quality oil.
  • the ⁇ conventional wet extraction method uses very high quantity of water which makes process very lengthy and difficult with respect to separation of oil and water and creates large quantity of effluent.
  • the enzyme composition of the present invention makes the process very efficient by requiring very less water and despite such less use of water the enzyme treated mixture is of liquid consistency and having much less viscosity thereby allowing the ease in separation of oil from leftover mass of fruit as well as water adding much ease in the processing and rendering process efficient and energy efficient as compared to the conventional methods.
  • the enzyme treated oil palm fruit mass is subjected to suitable separation means and method for separating oil and water from the fruit residue comprising fibers and entire seeds in case when the whole fruits are used. For example fiber mass and entire seeds are separated with the help of Vibrio-separator.
  • the oil and water mixture is subjected to suitable separation means to separate water and obtain oil.
  • the process as per the present invention provides crude palm oil with an increased yield of atleast 90%, preferably more than 95% or upto 98 % or even more.
  • the oil is clear, dark red in colour has good viscosity and bleachability index and has low FFA content (free fatty acid).
  • the enzyme composition as well as process of the present invention provides superior quality oil without any degradation of oil or adverse effect on the quality of oil.
  • the left over water containing the remainder of the enzyme is reused as such or after adjusting the amount of the enzyme as required for another batch for extracting oil.
  • the process of the present invention is made economically feasible by reusing left over water containing enzyme. This has huge economic advantage and helps in rapid reduction of costly enzymes. It also helps in recycling the water and thus making whole process effluent free. Thus, the present invention process is advantageous over the current commercially used process which leaves behind huge effluent and creates environmental hazards.
  • the enzyme composition and the process of the present invention of extracting ⁇ ! from oil palm fruits is advantageous and superior over the hitherto known methods on account of requiring less water during processing, adding much ease during processing, minimizing or avoiding harsh treatment, being less energy intensive, reducing time, not having any detrimental effect on the quality of oil and increasing yield.
  • Example lA Comparative Enzyme Composition:
  • the comparative enzyme composition was formulated by blending exocellulase enzyme having activity range from 7500-15000 u/gm with percent ranging from 3.5% -6.5%, pectinase enzyme having activity 1000 - 2500 u/gm with the percent ranging from 1%- 2.5% with the inert filler liklactose/maltodextrin with Q.S.
  • Example IB Enzyme Composition of the Present Invention: The enzyme composition as per the preferred embodiment present invention was formulated by blending exocellulase having activity range from 7500-15000 u/gm with percent ranging from 3.5% -6.5%, pectinase enzyme having activity 1000 - 2500 u/gm with the percent ranging from 1%- 2.5%, mannanase enzyme having activity ranging from 15000-25000 u/gm with percent ranging from 35%-55%, glucanase enzyme having activity range from 750 -2500 u/gm with percent ranging from 0.5% -1.5%, with inert filler like lactose/maltodextrin with percent ranging from 20-30%.
  • Example 2A Using comparative enzyme composition as per Example 1A.
  • Oil palm fruits were initially checked for their oil content by standard Soxhlet method and was found to have oil content of 65.99%. Three sets 1.4 kg each were taken for oil extraction. Palm fruits of all three sets were cleaned and steam cooked for 90 minutes at 146 degree Celsius for 3.4 kg pressure. The steamed fruits were crushed in the mixer and mashed mass was mixed with 1.4 Itr of water. To set 2A enzyme composition of Example 1A was added at the dose of 1.5%, to set 2B, enzyme composition of the present invention as per Example IB was added at the dose of 1.5% and to set 2C no enzyme composition was added. Mixture of three sets were incubated at 55 degrees for 2 Hours.
  • Set 2A and 2B were allowed to stand for 80 to 90 c for 15 min and then subjected to centrifugation to separate mass containing fibers with seed from oil and water layer. However for set 2C control, 30% more water was added on w/w basis to make it liquid as it being very thick and viscous mass before subjecting it to centrifugation step. The quantity of oil content was measured, FFA (free fatty acid) content and deterioration of bleachability index of oil were measured and sugar in the water layer was measured by AOAC method.
  • the deterioration of Bleach ability index(DOBI) value was compared with the standard values as below for the palm oil which is the numerical ratio of the spectrophotometer absorbance at 446nm to absorbance at 269nm.
  • the DOBI value of the oil obtained as per the present invention is more than 3.24 and hence the palm oil is of superior grade.

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Abstract

Composition enzymatique et procédé pour l'extraction d'huile à partir de fruits du palmier à huile, en particulier de la partie mésocarpe ou de fruits du palmier à huile contenant leur mésocarpe, avec une efficacité accrue et un rendement amélioré atteignant au moins 90%. Ledit procédé consiste à faire macérer le mélange contenant la partie mésocarpe écrasée ou les fruits écrasés contenant leur mésocarpe, de l'eau et une composition enzymatique contenant des enzymes ayant une activité exocellulolytique, pectinolytique, mannanolytique et glucanolytique, à une température comprise entre 25 et 65 degrés Celsius pendant au moins 60 à 120 minutes, puis à séparer l'huile de palme de l'eau et de la masse traitée par les enzymes.
PCT/IN2011/000491 2010-07-23 2011-07-25 Composition enzymatique et procédé pour l'extraction d'huile à partir des fruits du palmier à huile WO2012011130A2 (fr)

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IN2108/MUM/2010 2010-07-23
IN2108MU2010 2010-07-23

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WO2012011130A2 true WO2012011130A2 (fr) 2012-01-26
WO2012011130A3 WO2012011130A3 (fr) 2012-03-15
WO2012011130A8 WO2012011130A8 (fr) 2012-07-19

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Cited By (14)

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WO2016162510A1 (fr) * 2015-04-08 2016-10-13 Novozymes A/S Procédé d'extraction d'huile de palme à l'aide d'enzymes
WO2016162507A1 (fr) * 2015-04-08 2016-10-13 Novozymes A/S Procédé d'extraction d'huile de palme à l'aide d'enzymes
AU2015101377B4 (en) * 2014-12-19 2017-02-02 Dupont Nutrition Biosciences Aps Method of treating a palm fruit or a palm fruit liquid
WO2017182666A1 (fr) 2016-04-22 2017-10-26 Novozymes A/S Utilisation de la phospholipase c pour le broyage d'huile de palme
WO2017182665A1 (fr) 2016-04-22 2017-10-26 Novozymes A/S Extraction d'huile de palme à l'aide d'enzymes et d'un stérilisateur continu
WO2017202983A1 (fr) 2016-05-24 2017-11-30 Novozymes A/S Appareil et procédé d'application d'une préparation enzymatique
CN108048202A (zh) * 2017-10-23 2018-05-18 中国热带农业科学院椰子研究所 一种富含类胡萝卜素的棕榈油的制备方法
WO2019022592A1 (fr) * 2017-07-28 2019-01-31 Ng Say Bock Procédé d'extraction de produits à partir de fruits de palmier à huile
WO2019038418A1 (fr) 2017-08-25 2019-02-28 Novozymes A/S Extraction d'huile de palme brute assistée par des enzymes
WO2019068850A1 (fr) 2017-10-06 2019-04-11 Novozymes A/S Extraction d'huile de palme brute assistée par des enzymes
WO2020125985A1 (fr) * 2018-12-20 2020-06-25 Dutch Clean Tech Group B.V. Récupération d'huile végétale à partir de sources naturelles
US11053490B2 (en) 2014-12-19 2021-07-06 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
US11180786B2 (en) 2016-11-25 2021-11-23 Novozymes A/S GH10 xylanase, GH62 arabinofuranosidase, milling process and other application
US11649298B2 (en) 2015-11-26 2023-05-16 Novozymes A/S Wet milling process

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US20080064064A1 (en) * 2006-07-18 2008-03-13 Direvo Biotech Ag Mannanases
US20080260894A1 (en) * 2007-04-10 2008-10-23 Allan Lim Use of a multi-protease system to improve the protein digestibility of animal feeds containing vegetable meals
US20100167351A1 (en) * 2008-12-29 2010-07-01 Aharon Eyal Integrated Methods for Processing Palm Fruit Bunches

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080064064A1 (en) * 2006-07-18 2008-03-13 Direvo Biotech Ag Mannanases
US20080260894A1 (en) * 2007-04-10 2008-10-23 Allan Lim Use of a multi-protease system to improve the protein digestibility of animal feeds containing vegetable meals
US20100167351A1 (en) * 2008-12-29 2010-07-01 Aharon Eyal Integrated Methods for Processing Palm Fruit Bunches

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11926852B2 (en) 2014-12-19 2024-03-12 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
AU2015101377B4 (en) * 2014-12-19 2017-02-02 Dupont Nutrition Biosciences Aps Method of treating a palm fruit or a palm fruit liquid
US11053490B2 (en) 2014-12-19 2021-07-06 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
US11788079B2 (en) 2014-12-19 2023-10-17 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
WO2016162507A1 (fr) * 2015-04-08 2016-10-13 Novozymes A/S Procédé d'extraction d'huile de palme à l'aide d'enzymes
WO2016162510A1 (fr) * 2015-04-08 2016-10-13 Novozymes A/S Procédé d'extraction d'huile de palme à l'aide d'enzymes
AU2016245215B2 (en) * 2015-04-08 2018-11-08 Novozymes A/S Process for extraction of palm oil using enzymes
AU2016245218B2 (en) * 2015-04-08 2018-11-08 Novozymes A/S Process for extraction of palm oil using enzymes
US11649298B2 (en) 2015-11-26 2023-05-16 Novozymes A/S Wet milling process
US11987649B2 (en) 2015-11-26 2024-05-21 Novozymes A/S Wet milling process
WO2017182666A1 (fr) 2016-04-22 2017-10-26 Novozymes A/S Utilisation de la phospholipase c pour le broyage d'huile de palme
WO2017182667A1 (fr) 2016-04-22 2017-10-26 Novozymes A/S Extraction d'huile assistée par enzyme utilisant un condensat de stérilisateur
AU2017252228B2 (en) * 2016-04-22 2021-12-02 Novozymes A/S Enzyme-assisted oil extraction using sterilizer condensate
WO2017182665A1 (fr) 2016-04-22 2017-10-26 Novozymes A/S Extraction d'huile de palme à l'aide d'enzymes et d'un stérilisateur continu
WO2017202983A1 (fr) 2016-05-24 2017-11-30 Novozymes A/S Appareil et procédé d'application d'une préparation enzymatique
US11180786B2 (en) 2016-11-25 2021-11-23 Novozymes A/S GH10 xylanase, GH62 arabinofuranosidase, milling process and other application
WO2019022592A1 (fr) * 2017-07-28 2019-01-31 Ng Say Bock Procédé d'extraction de produits à partir de fruits de palmier à huile
WO2019038418A1 (fr) 2017-08-25 2019-02-28 Novozymes A/S Extraction d'huile de palme brute assistée par des enzymes
WO2019068850A1 (fr) 2017-10-06 2019-04-11 Novozymes A/S Extraction d'huile de palme brute assistée par des enzymes
CN108048202A (zh) * 2017-10-23 2018-05-18 中国热带农业科学院椰子研究所 一种富含类胡萝卜素的棕榈油的制备方法
WO2020125985A1 (fr) * 2018-12-20 2020-06-25 Dutch Clean Tech Group B.V. Récupération d'huile végétale à partir de sources naturelles

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