Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
  • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
  • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
  • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
  • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
  • G01N2333/70525—ICAM molecules, e.g. CD50, CD54, CD102
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2800/00—Detection or diagnosis of diseases
  • G01N2800/36—Gynecology or obstetrics
  • G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour

Definitions

• the invention relates to a medical detecting tool, in particular to a detecting tool and a detecting box for detecting a premature Rupture of Fetal Membrane (probe of PROM) in a pregnant woman.
• a medical detecting tool in particular to a detecting tool and a detecting box for detecting a premature Rupture of Fetal Membrane (probe of PROM) in a pregnant woman.
• ROM Fetal Membrane
• PROM premature rupture of membranes
• ROM membrane rupture that occurs before labor
• PROM premature rupture of membranes
• the incidence of PROM is approximately 10%; the pre-term (37 weeks before gestation) PROM incidence is 2-3.5%.
• Premature rupture of membranes is a major cause of prenatal and postpartum complications in obstetric patients. It is the main cause of preterm birth and neonatal intensive care unit. Because medical staff has to balance the risk of gestational age and intrauterine and pregnant women infection and fetal lung development, the management cost of premature rupture of membranes is high, difficult, accurate and timely diagnosis of pregnant women with fetal membranes Breaking is crucial.
• the gold standard is an accurate method for diagnosing premature rupture of fetal membranes, that is, injecting a dye (such as methylene blue) into the amniotic cavity of a pregnant woman, and if the liquid in the vagina is colored by the dye, the membrane can be diagnosed as a membrane.
• a dye such as methylene blue
• the use of gold standard testing is difficult to operate clinically, and causes pain in patients, so it is rarely used clinically.
• Clinically the medical history is usually used, the patient complains that the vagina has amniotic fluid outflow, the pH of the vaginal secretions becomes alkaline (normal vaginal secretion pH is 4.5 ⁇ 5.5), and the amniotic fluid crystal appears in the vaginal discharge smear under the microscope.
• other methods although these methods are simple, the accuracy and sensitivity are low.
• Intercellular Adhesion Molecule 1 (Intercellular Adhesion Molecule 1 , the tube called ICAM-1, hereinafter referred to as "ICAM-1" indicates intercellular adhesion molecule-1) belongs to the immunoglobulin superfamily of adhesion molecules, which is a molecular weight of 76kD ⁇ 114 kD single-chain glycoprotein. ICAM-1 is widely expressed on the surface of non-blood-derived cells such as vascular endothelial cells, thymic epithelial cells and other types of epithelial cells, and the surface of blood-derived cells such as T lymphocytes, B lymphocytes and dendritic cells. Fibroblast Surface, related to the infiltration of lymphocytes.
• the object of the present invention is to overcome the deficiencies of the prior art, and to provide a rapid detection tool and a detection box for premature rupture of membranes using ICAM-1 as a detection index, so as to achieve non-invasive detection and quickly obtain detection results.
• ICAM-1 is one of the major proteins present in amniotic fluid in the third trimester, and the content is 13.08 ⁇ 6.11 ng/mL.
• amniotic fluid will leak from the amniotic cavity to the vagina, and ICAM-1 in amniotic fluid will appear in Cervical-Vaginal Fluid (CVF).
• CVF Cervical-Vaginal Fluid
• Protein chip screening experiments found: The confirmed PROM maternal compared with the confirmed non-PROM maternal (the maternal age matched with the gestational age, no underlying disease, no pregnancy comorbidity), vaginal secretion / cervical mucus sample ICAM- The concentration of 1 has a significant difference. Therefore, ICAM-1 can be used as a test indicator for premature rupture of membranes.
• the rapid detection tool for premature rupture of membranes using ICAM-1 as a detection index includes a village tablet, an absorbent pad, a nitrocellulose membrane, a gold standard pad and a sample pad, the absorbent pad, a nitrocellulose membrane, a gold standard
• the pad and the sample pad are successively attached to the village piece from top to bottom, and the gold standard pad is partially covered by the sample pad;
• the nitrocellulose membrane is provided with a goat anti-human intercellular adhesion molecule-1 (goat anti-human ICAM-1) a detection line formed by polyclonal antibody coating and a control line formed by polyclonal antibody of goat anti-mouse immunoglobulin G (goat anti-mouse IgG, Immunoglobulin G, IgG), the detection lines are located under the control line and they are There is a gap between the two;
• the gold standard pad is a water-absorbent fiber coated with a monoclonal antibody anti-human intercellular adhesion molecule-1 monoclonal antibody conjug
• the rapid detection box for premature rupture of membranes with ICAM-1 as a detection index comprises a box body and the above-mentioned detecting tool loaded in the box body; the inner cavity of the box body matches the shape and size of the detecting tool
• the top wall of the box body is provided with a detecting tool insertion hole, and the side wall is provided with a sampling hole and an observation hole, and the setting position of the sample hole is corresponding to the position of the sample pad after the detecting tool is loaded into the box body,
• the position of the observation hole is corresponding to the position of the detection line and the control line after the detection tool is loaded into the casing.
• the width of the detection line and the control line is preferably 0.8 mm to 1 mm;
• the spacing between the detection line and the control line is preferably 10 mm to 12 mm.
• the shape of the detecting tool is not critical, and the shape is preferably a rectangular strip from the viewpoint of cost saving and convenient production.
• the water absorbing pad is a pad body made of absorbent paper, and the sample pad is a pad body made of water absorbing fiber, that is, a water absorbing fiber pad.
• the rapid detection tool for premature rupture of membranes using ICAM-1 as a detection index is as follows:
• Sheep anti-human ICAM-1 polyclonal antibody (purchased from R&D) and goat anti-mouse IgG polyclonal antibody (purchased from Shanghai Paikun Bioengineering Co., Ltd.) Coating method on nitrocellulose membrane See paper "AFP immune layer” Development of the test strip” (Hou Huiren, Wu Fengbo, Chen Jian, Liu Yibing, He Youfeng, "Isotopes", 2000, 13(3): 124-126)
• the concentration of goat anti-human ICAM-1 polyclonal antibody working solution is 1 ⁇
• the concentration of 3 mg/ml goat anti-mouse IgG polyclonal antibody working solution is 1 ⁇ 3 mg/ml.
• the chloroauric acid is dissolved in the trihydrated water to form a chloroauric acid aqueous solution having a chloroauric acid concentration of 0.01 g/100 ml; the prepared aqueous solution of chloroauric acid is heated to boiling, and the concentration is added to a concentration of 9 g to 10 g under stirring.
• mouse anti-human ICAM-1 monoclonal antibody purchased from R&D Company
• colloidal gold prepared in step (1) mix and mix well, add bovine serum albumin (BSA, Roche), stir and mix evenly, add concentration
• BSA bovine serum albumin
• concentration For 10 g/100 ml of NaCl solution, the amount of mouse anti-human ICAM-1 monoclonal antibody is limited to 200/100 ml of mouse anti-human IC AM-1 monoclonal antibody in colloidal gold solution, bovine serum white
• bovine serum white The amount of protein added is limited to 250 mg / 100 ml of bovine serum albumin in the colloidal gold solution.
• the amount of NaCl solution is limited to 1 g/100 ml of the concentration of NaCl in the colloidal gold solution;
• the conditions of 2,000 rpm, 4,000 rpm, and 10,000 rpm were each centrifuged for at least 10 minutes, and the obtained isolated product was a gold-labeled mouse anti-human ICAM-1 monoclonal antibody conjugate, and the conjugate was dissolved in pH.
• the gold-labeled mouse anti-human ICAM-1 monoclonal prepared in step (2) is immersed in a concentration of 0.5 g/100 ml of bovine serum albumin (BSA, Roche) solution at 22 ⁇ 25 °C for at least 1 hour.
• BSA bovine serum albumin
• the antibody conjugate is coated on the water-absorbing fiber (see the paper "The development of AFP immunochromatographic test strip", Hou Huiren, Wu Fengbo, Chen Jian, Liu Yibing, He Youfeng, "Isotopes", 2000, 13 (3): 124- 126), that is, the gold standard pad is obtained.
• the sheet-like absorbent pad 2, the nitrocellulose membrane 5 coated with the detection line and the control line, the gold standard pad 6 and the sample pad 7 were adhered to the sheet-like village piece 1, and then cut into rectangular strips.
• a clear purple-red line can be seen on the control line to prove that the test strip is effective; when a clear purple-red line is visible on the detection line, the result is judged to be positive (ie, the membrane is generated) Premature break); If there is no color line on the test line, it is judged to be negative (ie, no premature rupture of membranes occurs).
• the present invention provides a detection tool using a new detection index for the detection of premature rupture of membranes.
• the sampling test is performed on the confirmed fetal membrane rupture group and the pregnant woman whose undetected fetal membrane is not broken. The test results show that the sensitivity of the test kit is about 96% and the specificity is about 91%.
• test cartridge of the present invention Using the test cartridge of the present invention, it is only 10-15 minutes to test a sample, and the detection speed is greatly improved compared with the conventional detection methods in clinical practice.
• test sample is the vaginal secretion of a pregnant woman, it is a non-invasive test.
• the detection tool and the detection box of the invention have the advantages of simple processing, low production cost and convenient industrial production.
• test tool and test kit of the present invention are suitable for all pregnant women, especially those who have premature birth risk and abdominal damage.
• Figure 2 is a left side view of Figure 1;
• FIG. 3 is a schematic view showing a structure of a rapid detection box for preventing premature rupture of membranes using ICAM-1 as an index of detection according to the present invention
• Figure 4 is a plan view of Figure 3;
• Figure 5 is a cross-sectional view taken along line A-A of Figure 3;
• Fig. 6 is a schematic view showing the structure of a rapid test cartridge for premature rupture of membranes using ICAM-1 as a detection index according to the present invention.
• Example 1 The structure, manufacturing method and using method of the rapid detection tool and the detection box for premature rupture of membranes with ICAM-1 as the detection index according to the present invention will be further described below with reference to the accompanying drawings.
• Example 1 The structure, manufacturing method and using method of the rapid detection tool and the detection box for premature rupture of membranes with ICAM-1 as the detection index according to the present invention will be further described below with reference to the accompanying drawings.
• the rapid detection tool for premature rupture of membranes using ICAM-1 as a detection index is shown in FIG. 1 and FIG. 2, and the shape thereof is a rectangular strip, and the structure thereof includes a village sheet 1, an absorbent pad 2, and a nitrocellulose membrane. 5.
• the gold standard pad 6 and the sample pad 7, the water absorption pad, the nitrocellulose membrane, the gold standard pad and the sample pad are successively attached to the village piece from top to bottom, and the gold standard pad is partially covered by the sample pad.
• Village film 1 is made of medical plastic high whiteness PVC sheet, coated with pressure sensitive adhesive, which is inert to bioactive molecules; absorbent pad 2 is made of absorbent paper; nitrocellulose membrane 5 is provided with goat anti-human ICAM- 1 Polyclonal antibody-coated test line 4 and control line 3 formed by goat anti-mouse IgG polyclonal antibody, detection line 4 is located under control line 3, and their width is 1 mm (or 0.8 mm or 0.9) Mm ), the spacing between them is 10mm (or 11mm or 12mm); gold standard pad 6 is coated with gold standard mouse anti-human ICAM-1 monoclonal antibody a glass fiber film or a polyester fiber film of a bulk bond; the sample pad is a pad body made of a glass fiber film or a polyester fiber film.
• mice anti-human ICAM-1 monoclonal antibody purchased from R&D
• colloidal gold prepared in step (1)
• BSA bovine serum albumin
• a NaCl solution having a concentration of 10 g/100 ml is added, and the amount of the NaCl solution is limited to a concentration of 1 g/100 ml of NaCl in the colloidal gold solution.
• the obtained product was a gold-labeled mouse anti-human ICAM-1 monoclonal antibody conjugate, and the conjugate was obtained.
• phosphate buffer solution of pH 7.2, stored at 4 ° C for use, the phosphate buffer solution contains sodium azide, the concentration of sodium azide is 90 mg / 100 ml;
• BSA bovine serum albumin
• the gold-labeled mouse anti-human ICAM-1 monoclonal antibody conjugate prepared in the step (2) is coated on the glass fiber membrane or the polyester fiber membrane (see the coating method).
• the shape and configuration of the rapid premature rupture test cartridge using ICAM-1 as a detection index are as shown in Fig. 6, and are composed of a casing 8 and a detecting tool 12 housed in the casing.
• the detecting tool 12 is a detecting tool of the shape and configuration described in Embodiment 1.
• the casing 8 is made of medical plastic.
• the outer shape is a rectangular parallelepiped.
• the inner cavity is matched with the shape and size of the detecting tool, and the top wall is provided with a detecting tool insertion hole 11 .
• the side wall is provided with a sample insertion hole 10 and an observation hole 9, and the installation position of the sample insertion hole 10 corresponds to the position of the sample pad 7 after the detection tool is loaded into the case body, and the installation position and the detection tool of the observation hole 9 are provided.
• the position of the detection line 4 and the control line 3 corresponds to the position after the cassette is loaded.
• the test kit was sealed and sealed with an aluminum foil bag with a desiccant.
• the method of using the detection box and the detection principle are as follows:
• the sample to be tested (pregnancy vaginal secretion/cervix mucus) is added from the sample well 10 of the cartridge 8 to the sample pad 7 of the detection tool, and the sample to be tested moves upward from the sample pad 7 along the strip detection tool due to the principle of chromatography.
• the gold standard pad 6 when moved to the gold standard pad 6, dissolve the gold-labeled mouse anti-human ICAM-1 monoclonal antibody conjugate, and continue to move upward with the gold-labeled mouse anti-human IC AM-1 monoclonal antibody, when moving to nitrocellulose Detection line 4 on the membrane, if the ICAM-1 antigen is present in the sample to be tested, the goat anti-human ICAM-1 polyclonal antibody on the detection line 4 and the gold-labeled mouse anti-human ICAM-1 monoclonal antibody and the ICAM in the sample The -1 antigen will form a purple-red sandwich immune complex on the test line. A clear purple-red line is visible on the test line. The result is positive.

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• 2010
  • 2010-06-02 CN CN201010189683.1A patent/CN101871943B/zh active Active
• 2011
  • 2011-05-20 WO PCT/CN2011/074383 patent/WO2011150752A1/zh active Application Filing
  • 2011-05-20 EP EP11789131.7A patent/EP2579041B1/en not_active Not-in-force

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