WO2011132890A3 - 바이오 에탄올 생산 향상용 형질전환체 및 그 균주를 이용한 에탄올 생성방법 - Google Patents

바이오 에탄올 생산 향상용 형질전환체 및 그 균주를 이용한 에탄올 생성방법 Download PDF

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Publication number
WO2011132890A3
WO2011132890A3 PCT/KR2011/002712 KR2011002712W WO2011132890A3 WO 2011132890 A3 WO2011132890 A3 WO 2011132890A3 KR 2011002712 W KR2011002712 W KR 2011002712W WO 2011132890 A3 WO2011132890 A3 WO 2011132890A3
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WIPO (PCT)
Prior art keywords
transformant
enhancing
strain
producing ethanol
bioethanol production
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PCT/KR2011/002712
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English (en)
French (fr)
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WO2011132890A2 (ko
WO2011132890A9 (ko
Inventor
한성옥
유경옥
김승욱
Original Assignee
고려대학교 산학협력단
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Priority claimed from KR1020100035853A external-priority patent/KR20110116438A/ko
Priority claimed from KR1020110003479A external-priority patent/KR101262999B1/ko
Priority claimed from KR1020110003476A external-priority patent/KR101259956B1/ko
Application filed by 고려대학교 산학협력단 filed Critical 고려대학교 산학협력단
Priority to US13/641,946 priority Critical patent/US8772000B2/en
Publication of WO2011132890A2 publication Critical patent/WO2011132890A2/ko
Publication of WO2011132890A3 publication Critical patent/WO2011132890A3/ko
Publication of WO2011132890A9 publication Critical patent/WO2011132890A9/ko

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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
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    • C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)
    • C12Y101/05003—Glycerol-3-phosphate dehydrogenase (1.1.5.3)
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    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/0101—(2E,6E)-Farnesyl diphosphate synthase (2.5.1.10), i.e. geranyltranstransferase
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    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
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    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
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    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
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    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
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  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
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  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 글리세롤을 발효원으로 이용하도록 조작되어진 사카로마이세스 세레비지애 (Saccharomyces cerevisiae)의 글리세롤 생산 유전자의 결손을 통하여 글리세롤 생산을 저해시키거나, TATA 결합단백질 SPT3와 15의 과발현을 통하여 바이오 에탄올 생산능이 향상된 형질전환체 및 그 형질전환체를 이용한 에탄올 생산방법에 관한 것이다.
PCT/KR2011/002712 2010-04-19 2011-04-15 바이오 에탄올 생산 향상용 형질전환체 및 그 균주를 이용한 에탄올 생성방법 WO2011132890A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/641,946 US8772000B2 (en) 2010-04-19 2011-04-15 Transformant for enhancing bioethanol production, and method for producing ethanol by using said strain

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR1020100035853A KR20110116438A (ko) 2010-04-19 2010-04-19 바이오 에탄올 생산 향상용 글리세롤 생산 유전자 결손 변이체 및 그 균주를 이용한 에탄올 생성방법
KR10-2010-0035853 2010-04-19
KR10-2011-0003476 2011-01-13
KR1020110003479A KR101262999B1 (ko) 2011-01-13 2011-01-13 바이오 에탄올 생산 향상용 피루브산 탈카르복실산 효소와 알코올 탈수소화 효소를 코딩하는 유전자가 도입된 형질전환체 및 그 균주를 이용한 에탄올 생성방법
KR1020110003476A KR101259956B1 (ko) 2011-01-13 2011-01-13 바이오 에탄올 생산 향상용 ｔａｔａ 결합 단백질 ｓｐｔ3 와 15가 유전자 삽입된 형질전환체 및 그 균주를 이용한 에탄올 생성방법
KR10-2011-0003479 2011-01-13

Publications (3)

Publication Number Publication Date
WO2011132890A2 WO2011132890A2 (ko) 2011-10-27
WO2011132890A3 true WO2011132890A3 (ko) 2012-05-10
WO2011132890A9 WO2011132890A9 (ko) 2012-06-14

Family

ID=44834609

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002712 WO2011132890A2 (ko) 2010-04-19 2011-04-15 바이오 에탄올 생산 향상용 형질전환체 및 그 균주를 이용한 에탄올 생성방법

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US (1) US8772000B2 (ko)
WO (1) WO2011132890A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2506178A (en) * 2012-09-25 2014-03-26 Vib Vzw Mutant yeast strain with decreased glycerol production
AR097479A1 (es) * 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células convertidoras de glicerol y ácido acético con un transporte de glicerol mejorado
EP3169768B1 (de) * 2014-07-14 2018-09-26 Jacobs University Bremen GmbH Gentechnisch veränderte hefe mit verbessertem glycerol-katabolismus

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2060632A1 (en) * 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CAO, L. ET AL.: "Overexpression ofGLTl in fpsl0gpd0 mutant for optimum ethanol formation by Saccharomyces cerevisiae", BIOMOLECULAR ENGINEERING., vol. 24, no. 6, 23 October 2007 (2007-10-23), pages 638 - 642 *
HOU, L. ET AL.: "Effect of overexprssion of transcription factors on the fermentation properties of Saccharomyces cerevisiae industrial strains", LETTERS IN APPLIED MICROBIOLOGY., vol. 49, no. 1, 17 April 2009 (2009-04-17), pages 14 - 19 *
OHTA, K. ET AL.: "Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 57, no. 4, April 1991 (1991-04-01), pages 893 - 900 *
PETER SMITS, H. ET AL.: "Simultaneous overexpression of enzymes of the lower part of glycolysis can enhance the fermentative capacity of Saccharomyces cerevisiae", YEAST., vol. 16, no. 14, October 2000 (2000-10-01), pages 1325 - 1334 *
YU, K. O. ET AL.: "Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae", BIORESOURCE TECHNOLOGY., vol. 101, no. 11, 9 February 2010 (2010-02-09), pages 4157 - 4161 *

Also Published As

Publication number Publication date
US20130109070A1 (en) 2013-05-02
US8772000B2 (en) 2014-07-08
WO2011132890A2 (ko) 2011-10-27
WO2011132890A9 (ko) 2012-06-14

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