WO2011110141A1 - Unité de pipetage et pipeteur comprenant une telle unité de pipetage, et pointe de pipette destinée à être utilisée avec un tel pipeteur - Google Patents

Unité de pipetage et pipeteur comprenant une telle unité de pipetage, et pointe de pipette destinée à être utilisée avec un tel pipeteur Download PDF

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Publication number
WO2011110141A1
WO2011110141A1 PCT/DE2010/050010 DE2010050010W WO2011110141A1 WO 2011110141 A1 WO2011110141 A1 WO 2011110141A1 DE 2010050010 W DE2010050010 W DE 2010050010W WO 2011110141 A1 WO2011110141 A1 WO 2011110141A1
Authority
WO
WIPO (PCT)
Prior art keywords
receiving
pipette
pipette tip
pipetting
pipettor
Prior art date
Application number
PCT/DE2010/050010
Other languages
German (de)
English (en)
Inventor
Thomas Moore
Original Assignee
Cybio Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cybio Ag filed Critical Cybio Ag
Priority to PCT/DE2010/050010 priority Critical patent/WO2011110141A1/fr
Publication of WO2011110141A1 publication Critical patent/WO2011110141A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices

Definitions

  • Pipetting unit and pipettor comprising such a pipette unit, and pipette tip for use with such a pipettor
  • the invention relates to a pipetting unit and a pipettor with at least one such pipetting unit.
  • the pipettors are so-called bubble pad pipettes and can be distinguished in single-channel and multi-channel pipettors. They can be operated both manually or as an electric motor.
  • a generic pipetting unit as well as a generic pipettor are known from JP 08117618 A.
  • pipettors comprise per specified in the name of the pipettor channel each have a pumping chamber and a piston guided therein, wherein the pumping chamber adjacent to a receiving device through which extends a connecting channel, via which the
  • the piston can be associated with a pipette belonging to the drive and control device and thus motorized lifted or lowered over a given piston stroke, which, by generating a negative pressure or overpressure within the pumping chamber, taken on the pipette tip liquid or can be delivered.
  • the piston stroke can also be effected manually.
  • the number of channels indicated in the designation of the pipettor corresponds to both the number of pumping chambers and the number of pipette tips which can be fastened at the same time to the pipettor.
  • the number of channels does not coincide with the number of pumping chambers, since a plurality of pumping chambers each with a pipette tip or a pumping chamber via a manifold with several pipette tips are in communication ,
  • the number of channels is always consistent with the number of pipette tips that can be attached at the same time to the pipettor.
  • Multi-channel pipettor be understood.
  • a single-channel pipettor accordingly comprises a pipetting unit consisting of one
  • a multichannel pipettor accordingly consists of a number of pipetting units equal to the stated number of channels.
  • sample plates have this suitable 8x12 (equal to 96), 16x24 (equal to 384) or meanwhile also 32x48 (equal to 1536) wells.
  • Their external dimensions are the same to be compatible with laboratory equipment and handling equipment. As a result, the center distances between the pits become smaller as the number of pits on a sample plate increases. Consequently, the diameter and thus the possible receiving volume of the wells must be reduced.
  • a pipette tip represents a "usually rotationally symmetrical hollow body, with a discharge opening, via which a liquid can be received and discharged, and a receiving opening, via which the cavity enclosed by the hollow body via the receiving device with the pumping chamber in
  • a pipette pipette tip with the discharge opening is immersed in the liquid to be transferred and by generating a negative pressure in the associated with the pipette tip pumping chamber, a certain volume of liquid is added to the pipette tip (Void volume).
  • the maximum possible intake volume is limited by the maximum piston stroke. In practice, however, only such a volume should be taken up as corresponds to the capacity of the pipette tip in order to avoid contamination of the pipette unit.
  • the pipette tips are usually designed as disposable items, also called disposable items, and are replaced after use.
  • the pipette tip is attached via a quick release, non-positive connection to the receiving device.
  • the receiving unit of known from the prior art pipetting is often designed so that only pipette tips same Anschlußgeometrie and connection dimensions can be attached to it.
  • a trained around the receiving opening of the pipette tip end face is positively connected to a formed on the receiving device end face.
  • the frictional attachment of the pipette tip can be carried out by sliding onto the receptacle formed as a receiving shaft on which is formed for this purpose a receiving surface in the form of an outer cone or an outer cylinder.
  • the pipette tip With the application of a correspondingly high counterforce by means of an existing Abwerfmechanismus (ejector), the pipette tip can be automatically released from the receiving device.
  • pipette tips are suitable for this purpose in an adjacent to the receiving opening area, hereinafter called pipette collar formed in the form of an equal or slightly smaller dimensioned inner cone or inner cylinder.
  • a pipette unit can be used only with pipette tips, which have a matching pipette collar, with a connection geometry suitable for the receiving surface and matching connection dimensions.
  • the former solutions include a multichannel pipettor as disclosed in US Pat. No. 6,841,130 B2.
  • each other in a fixed grid channels are grouped together via an adapter plate so that they are in each case via a pipette tip with the liquid to be absorbed in combination.
  • So can z.
  • a 384 channel multichannel pipettor with an adapter plate connecting 4 channels each with a pipette tip can be adapted for use on a 96-well sample plate.
  • the maximum transfer volume and thus the possible range of the transfer volume which is also referred to as dynamic range, be significantly increased.
  • four combined channels and the related part of the adapter plate, to which a pipette tip can be attached form a pipetting unit.
  • the pipetting attachment described here represents an adapter between 4 channels of a multichannel pipettor and a tip (pipette tip). It has on one end face four mutually fixed Aufsteckhülsen (pipette collar), with which the adapter is placed on the free ends of the channels , and at the other end a connection piece for attaching a tip.
  • the axis of the tip passes through the center of the arrangement formed by the four channels.
  • the four channels combined in the adapter form a pipetting unit according to the invention.
  • the second-mentioned solutions include a liquid handling apparatus disclosed in Japanese Patent Laid-Open No. 08117618A.
  • This liquid handling device can be used like generic type pipetting units singly or in a plurality in a single-channel or multi-channel pipettor.
  • the peculiarity of the liquid handling device disclosed here is that it has a receiving adapter, in the context of the invention, a receiving shaft having two receiving surfaces for the attachment of pipette tips.
  • the receiving surfaces are designed as outer cones.
  • two cylindrical pumping chambers are formed within the liquid handling device with a different diameter, in each of which a piston is guided.
  • the two outer cones are each coaxially arranged around one of the two pumping chambers, connected to this.
  • the pumping chambers are for a different size.
  • Volume intake designed and optimized for pipette tips of different sizes, in order to be able to pipette alternatively via both pipette tips with high accuracy.
  • the pistons are connected to a common drive and control device so that always both pistons are moved simultaneously.
  • the invention is based on the object, a pipetting with a high Dynamic range for use with different pipette tips to create, which requires only a small horizontal space in the receiving surfaces for attaching the pipette tips, especially to form of a plurality of such pipetting a Mehrkanalpipettor, in which the center distances of infected pipette tips are low to each other.
  • the pipetting accuracy should not be affected.
  • the object of the invention is also to provide a pipettor, namely a Einkanalpipettor or a Mehrkanalpipettor with at least one pipetting unit with a high dynamic range for use with different pipette tips, which in the receiving surfaces of the pipette for attaching the pipette tips only a small horizontal space needed.
  • a pipettor namely a Einkanalpipettor or a Mehrkanalpipettor with at least one pipetting unit with a high dynamic range for use with different pipette tips, which in the receiving surfaces of the pipette for attaching the pipette tips only a small horizontal space needed.
  • a pipette tip is to be created, which can be used with a pipettor formed from at least one such pipette and can be dropped by an ejector.
  • the first object of the invention is achieved by a pipetting unit for use with various pipette tips having at least one pumping chamber in which a piston is guided, and a receiving shaft having at least one outer opening into which opens the at least one pumping chamber, wherein the receiving shaft at least has two rotationally symmetrical receiving surfaces for sealingly attaching different pipette tips, solved by the receiving surfaces are arranged coaxially to each other and having an outwardly increasing vertical distance to an axis of symmetry.
  • a first receiving surface is formed by an inner cylinder formed on the receiving shaft and at least one second receiving surface by an outer cylinder or outer cone formed on the receiving shaft.
  • all the receiving surfaces may advantageously be formed by formed on the receiving shaft outer cylinder or outer cones, which are arranged one above the other in the direction of the axis of symmetry, whereby only one type of pipette tips must be used.
  • the receiving surfaces designed as outer cylinder and outer cones have in their arrangement outwardly increasingly larger diameter, which pipette tips larger attachment dimensions can be plugged, which then usually have a larger capacity.
  • the pipetting unit has an equal number of pumping chambers, as receiving surfaces are formed on the receiving shaft and each open into a different opening.
  • a central outer opening present which lies on the axis of symmetry and between two receiving surfaces is provided in each case an off-center outer opening.
  • a pipette tip, which is placed on the innermost, first receiving surface, is therefore associated with only one pumping chamber.
  • a pipette tip placed on the second receiving surface, which is closest to the outside, stands with two
  • a pipette tip sitting on the xth receiving surface counting from inside to outside is also connected to x pumping chambers.
  • the sum of the pumping chamber volumes of the pumping chambers, which are connected to a pipette tip can thus be optimized for the capacity of this tip, if the pipette tips are dimensioned so that they have a larger receiving opening and thus larger connection dimensions, which means that they are located further out Recording surfaces are plugged, also have a larger capacity.
  • pipette tips of different capacities can be produced with the same connection dimensions.
  • a selection mechanism for selectively operating the individual pistons and individually controllable valves must be provided to the openings through which the pipette tip is in each case a pumping chamber in communication to release or close. It can be connected in this way each with a plugged tip different Pumpenkammem or a different number of Pumpkammem.
  • the need for the valves and the control of this and the selection mechanism make the pipetting unit more expensive and prone to failure.
  • a further object of the invention is achieved with a single-channel pipettor comprising a pipetting unit according to the invention or with a multichannel pipettor comprising a multiplicity of pipetting units, of which at least one pipetting unit is a pipetting unit according to the invention.
  • a multichannel pipettor may comprise eight pipetting units to which only one associated pipette tip may be attached in accordance with the prior art and which accordingly have a relatively small dynamic range while an additional pipetting unit of the present invention will have an increased dynamic range by alternative use with different pipette tips. has high pipetting accuracy.
  • a single-channel pipettor according to the invention or a multichannel pipettor according to the invention has an ejector with which a pipette tip which is connected to a receiving shaft can be stripped off from it.
  • an outer cylinder is formed around the receiving opening, which can be inserted into an inner cylinder formed on the receiving shaft, and a projection adjacent to the outer cylinder is provided with a facing the receiving opening plan face on which the ejector can be applied.
  • the pipetting unit will be explained in more detail below by way of example with reference to the drawing.
  • FIG. 1a is a schematic diagram of a pipetting unit provided for an optional
  • 2a shows a schematic diagram of a pipetting unit with two pumping chambers, at the one
  • FIG Fig. 3b shows another embodiment of a receiving shaft for the optional
  • FIG. 4a shows a receiving shaft to which a pipette tip of the second type is attached and a ejector arranged thereon
  • 4b shows a receiving shaft, on which a pipette tip of the first type in a second
  • FIG. 4c shows a receiving shaft on which a first type of pipette tip in a third
  • 5a shows a schematic diagram for a single-channel pipettor
  • FIG. 5b shows a schematic diagram for a multichannel pipettor.
  • FIG. 1a-1c show a first exemplary embodiment of a pipetting unit according to the invention and different pipette tips 7 attached to it.
  • Different pipette tips 7 are to be understood here as having a different connection geometry and / or different connection dimensions for attaching the pipette tip 7 to a receiving shaft 3.
  • pipette tips 7 that can be used with a pipetting unit according to the invention are subsequently distinguished on the basis of their different connection geometry in pipette tips of the first type 7.1 and pipette tips of the second type 7.2.
  • the pipette tips of the first type 7.1 and the second type 7.2 may have different connection dimensions and other different dimensions, such as different inner diameter and different lengths, depending on which areas of delivery volumes they are optimized.
  • the ratio of the adding volumes of the pumping chamber 1 and the pipette tip 7 used to the piston stroke determines the smallest deliverable volume of liquid and its accuracy. The highest pipetting accuracy is theoretically achieved when the volume of the pumping chamber 1 (at the maximum extended piston 2) is equal to the capacity of the pipette tip 7.
  • a pipette tip of the first type 7.1 is around the receiving opening 11, that is, at the end to which the pipette tip 7.1 is attached to the receiving shaft 3, designed as a joining surface outer surface, in particular a cylindrical / conical outer surface, with the pipette tip 7.1 can be inserted into a formed on the receiving shaft 3 inner cylinder / inner cone.
  • an inner surface configured as a joining surface is present around the receiving opening 11, in particular a conical or cylindrical inner surface with which the pipette tip 7.2 can be slipped onto an outer cone or outer cylinder formed on the receiving shaft 3.
  • pipette collars The area of the joining surfaces at the pipette tips 7 will be referred to below as pipette collars. So it's exactly this pipette collar that's one of the ordered receiving surface 6 matching connection geometry and matching connection dimensions.
  • a pipetting unit as shown in FIG. 1 a, comprises as essential to the invention
  • the pumping chamber 1 is formed by a cylindrical chamber in which the piston 2 can be raised and lowered in a sealed manner towards the chamber wall, and a connecting channel between the chamber and a central outer opening 4.1 formed on the receiving shaft 3.
  • the receiving shaft 3 has an outer receiving surface 6.2 for it aufsteckbare pipette tips second type 7.2, which is designed as an outer cone.
  • This outer receiving surface 6.2 includes a small cone angle a with the axis of symmetry 5 of the receiving shaft 3, which can be up to zero in the extreme case, whereby the outer cone merges into an outer cylinder.
  • the pipetting unit is usually placed on a pipette tip of the second type 7.2, which hangs vertically in a storage box, wherein the receiving shaft 3 is inserted into the receiving opening 11 of the pipette tip of the second type 7.2.
  • the pipette tip of the second type is slightly stretched 7.2 in the radial direction and stored a restoring force in the material of the pipette tip of the second type 7.2, which ensures a seal against the outer receiving surface 6.2.
  • a sealing rubber O-ring on the shaft
  • the pipette tip axis and the symmetry axis 5 of the receiving shaft 3 coincide after the pipette tip has been mounted.
  • the receiving shaft 3 has further coaxial receiving surfaces 6, which are numbered consecutively beginning from the achsnesten receiving surface 6.1 for further explanations.
  • the outer receiving surface 6.2 just described, as is customary in the prior art, is therefore referred to as a second receiving surface 6.2.
  • the receiving shaft 3 has two receiving surfaces 6.
  • the first and thus achsnste receiving surface 6.1 is formed by an end portion of the connecting channel, which extends through the receiving shaft 3 and ends in the central outer opening 4.1.
  • the tail represents a cylinder with a sufficiently large diameter to plug a pipette tip first type 7.1 can. An insertion takes place as already described plugging with the difference that a pipette tip of the first type 7.1 is radially compressed slightly when inserted.
  • a pipetting unit according to the first embodiment enables an alternative operation of the pipetting unit with pipette tips 7, which are provided for two different ranges of delivery volumes.
  • the receiving surfaces 6 are mutually coaxial about the axis of symmetry 5 of the receiving shaft 3, the relative arrangement of a single-channel pipettor or a MehrkanalpipettOTS to a sample plate, in which pipetting is maintained even after changing the size of the pipette tips 7.
  • used handling systems such as transport devices, lifter or gripper can be used unchanged and with the same control, regardless of which of the pipette tips 7 is used.
  • the two attachable pipette tips 7.1, 7.2 are alternatively in each case connected to the same pumping chamber 1.
  • both pipette tips 7.1, 7.2 which have a different capacity to be able to fill a maximum for efficient operation, the possible displacement volume of the pumping chamber 1 must be designed for the receiving volume of the larger pipette tip, whereby the accuracy of the liquid delivery via the smaller pipette tip 7.1 affected becomes.
  • a pipetting unit has two pumping chambers 1.
  • the first pumping chamber 1.1 opens like the first embodiment in the central outer opening 4.1 of the receiving shaft 3 and is thus in communication with each pipette tip 7, which is attached to the receiving shaft 3.
  • the displacement volume of the first pumping chamber 1.1 is optimized to the capacity of the innermost first pipette tip 7.
  • a second pumping chamber 1.2 opens into an off-center outer opening 4.2, which is present between the first and the second receiving surface 6.1, 6.2 in the receiving shaft 3, and is thus not in connection with an infected at the first receiving surface 6.1 pipette tip first type 7.1 but only with a pipette tip of the second type 7.2, which is attached to the second receiving surface 6.2.
  • These Pipette tip of the second type 7.2 is thus in communication with both pumping chambers 1.1, 1.2.
  • the displacement volume of the first and second pumping chambers 1.1, 1.2 is optimized to the capacity of the pipette tip of the second type 7.2.
  • Fig. 3a shows a Aufhahmetician 3 provided for three pipette tips 7 of different sizes.
  • a first pipette tip 7 is a pipette tip of the first type 7.1 and is inserted into the receiving shaft 3, while each additional pipette tip 7 is a pipette tip of the second type 7.2.
  • the pipetting unit can be used as part of a pipettor.
  • 5a shows a schematic diagram of a manually operable single-channel pipettor with the pipetting unit, comprising a pumping chamber 1 and a receiving shaft 3 and an ejector 9, which can be lowered counter to a spring force F in the direction of the axis of symmetry 5 'to one on the receiving shaft.
  • 3 stripped pipette tip 7 (not shown here) strip.
  • Fig. 5b shows a schematic diagram of a multi-channel pipettor.
  • the pumping chambers 1 are fixedly connected to one another to form a rigid arrangement of the pipetting units.
  • the guided in the pumping chambers 1, not shown piston 2, are moved, for example via a common actuator.
  • the ejector 9 is formed in the area around the pumping chambers 1 as a plane plate, which strips all lowering seated on the receiving shafts 3 pipette tips 7 (not shown here) when lowering.
  • Control units and a power supply or interfaces for connecting a power supply available are Control units and a power supply or interfaces for connecting a power supply available.
  • FIG. 3b shows a receiving shaft 3 provided for three pipette tips 7 of different sizes, all of which are pipette tips of the second type 7.2.
  • the receiving surfaces 6 are offset in the direction of the axis of symmetry 5 to each other, so that the receiving shaft 3 receives a stepped outer contour.
  • pipette tips are used as pipette tips of the second type 7.2. They have at their receiving opening 11 on an outer collar with a flat end face 8 extending radially to the tip axis.
  • an ejector 9, the z. B. by a lowerable, the receiving shaft 3 enclosing plan plate is formed, are applied to the front side of the outer collar, so that in a further lowering of the ejector 9, the pipette tip 7.2 is stripped from the receiving surface 6.2.
  • Such ejectors 9 are known from the prior art. So that the ejector 9 z. B. in one embodiment of a receiving shaft 3 of FIG.
  • Such an ejector 9 does not function in connection with pipette tips of the first type 7.1, if they are formed as shown in Fig. 2b.
  • a projection 10 may be formed on a pipette tip of the first type 7.1, which is designed so that an ejector 9, as shown in Fig. 4a, a pipette tip of the second type 7.2 can strip off when lowering with this projection 10 comes in contact and in a further lowering this entrains and thus strikes.
  • the projection 10 adjoins the outer periphery of the pipette tip 7 at a distance greater than the length of the joining surface to the receiving opening 11.
  • the pot-shaped projection 10.2 has the advantage that the ejector 9 for stripping a pipette tip of the first type 7.1 thus formed only has to be lowered by the same distance as for dropping a pipette tip of the second type 7.2.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

L'invention a pour objet une unité de pipetage comportant une tige de réception (3), sur laquelle des pointes de pipette (7) de tailles différentes peuvent être disposées en variante sur une pluralité de surfaces de réception (6.1, 6.2) disposées coaxialement autour d'un axe de symétrie (5), un même nombre de chambres de pompage (1.1, 1.2) que de surfaces de réception (6.1, 6.2) étant avantageusement disponibles, qui se jettent dans différentes ouvertures extérieures (4.1, 4.2) dans la tige de réception (3). Les pointes de pipette (7) peuvent par conséquent être reliées à un nombre différent de chambres de pompage (1.1, 1.2) en fonction de sur laquelle des surfaces de réception (6.1, 6.2) elles sont collées, de sorte que le volume de la pompe peut être optimisé sur la capacité des pointes de pipette (7) respectivement collées. La présente invention concerne également un pipeteur monocanal et un pipeteur multicanaux constitués d'au moins une unité de pipetage selon la présente invention ainsi qu'une pointe de pipette (7) appropriée pour une utilisation avec un tel pipeteur monocanal ou un tel pipeteur multicanaux.
PCT/DE2010/050010 2010-03-11 2010-03-11 Unité de pipetage et pipeteur comprenant une telle unité de pipetage, et pointe de pipette destinée à être utilisée avec un tel pipeteur WO2011110141A1 (fr)

Priority Applications (1)

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PCT/DE2010/050010 WO2011110141A1 (fr) 2010-03-11 2010-03-11 Unité de pipetage et pipeteur comprenant une telle unité de pipetage, et pointe de pipette destinée à être utilisée avec un tel pipeteur

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PCT/DE2010/050010 WO2011110141A1 (fr) 2010-03-11 2010-03-11 Unité de pipetage et pipeteur comprenant une telle unité de pipetage, et pointe de pipette destinée à être utilisée avec un tel pipeteur

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2572786A1 (fr) * 2011-09-20 2013-03-27 CyBio AG Dispositif de pipetage doté d'une tête de pipetage avec de nombreux canaux de pipetage disposés dans un modèle d'agencement
EP3560596A1 (fr) * 2018-04-23 2019-10-30 Eppendorf AG Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents

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US4679446A (en) * 1985-09-09 1987-07-14 Baxter Travenol Laboratories, Inc. Multi-volume displacement pipette
US5104375A (en) * 1989-10-16 1992-04-14 Johnson & Johnson Medical, Inc. Locking holder for a pair of syringes and method of use
WO2000027530A1 (fr) * 1998-11-06 2000-05-18 Rainin Instrument Co., Inc. Pointe de pipette a ejection facile
US20020053245A1 (en) * 1998-04-09 2002-05-09 Ccs Packard, Inc. Dispensing apparatus having means for loading pipette tips in a dispense head
WO2003086632A1 (fr) * 2002-04-08 2003-10-23 Yiu Felix H Pipette amelioree et embouts de pipette fermes hermetiquement de l'exterieur
WO2005039772A2 (fr) * 2003-10-24 2005-05-06 Schwartz H Donald Module de pipettage
DE202007013096U1 (de) * 2007-09-19 2009-03-05 Hamilton Bonaduz Ag Spritze mit wechselbarer Nadel

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Publication number Priority date Publication date Assignee Title
US3749084A (en) * 1971-05-03 1973-07-31 A Cucchiara Sequentially dispensing syringe with multiple needle assembly
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Publication number Priority date Publication date Assignee Title
EP2572786A1 (fr) * 2011-09-20 2013-03-27 CyBio AG Dispositif de pipetage doté d'une tête de pipetage avec de nombreux canaux de pipetage disposés dans un modèle d'agencement
CN103008039A (zh) * 2011-09-20 2013-04-03 西比欧公司 具备带多个排成一定格局的移液通道的移液头的移液装置
US9084993B2 (en) 2011-09-20 2015-07-21 Analytik Jena Ag Pipetting apparatus with a pipetting head comprising a multiplicity of pipetting channels disposed in an arrangement pattern
EP3560596A1 (fr) * 2018-04-23 2019-10-30 Eppendorf AG Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents
WO2019206879A1 (fr) 2018-04-23 2019-10-31 Eppendorf Ag Pipette pour utilisation avec une pointe de pipette et famille de pointes de pipette comprenant plusieurs pointes de pipette de type différent pour utilisation avec une pipette
CN112020394A (zh) * 2018-04-23 2020-12-01 埃佩多夫股份公司 与移液吸头一起使用的移液器和包括与移液器一起使用的不同型号的多种移液吸头的移液吸头套件
CN112020394B (zh) * 2018-04-23 2022-07-08 埃佩多夫股份公司 与移液吸头一起使用的移液器和包括与移液器一起使用的不同型号的多种移液吸头的移液吸头套件

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