EP2623203B1 - Distributeur capillaire - Google Patents

Distributeur capillaire Download PDF

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Publication number
EP2623203B1
EP2623203B1 EP20130150872 EP13150872A EP2623203B1 EP 2623203 B1 EP2623203 B1 EP 2623203B1 EP 20130150872 EP20130150872 EP 20130150872 EP 13150872 A EP13150872 A EP 13150872A EP 2623203 B1 EP2623203 B1 EP 2623203B1
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EP
European Patent Office
Prior art keywords
plunger
capillary
piston
inner cylinder
module
Prior art date
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Application number
EP20130150872
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German (de)
English (en)
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EP2623203A1 (fr
Inventor
Adrian Siemers
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Cybio AG
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Cybio AG
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0265—Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves

Definitions

  • a dispenser described here consists of a plurality of capillaries which are open on both sides, a compressed air supply, a valve for switching the compressed air, a pressure chamber consisting of an upper part with compressed air inlet and a lower part in which the capillaries are held. Upper and lower part of the pressure chamber are sealed against each other, so that when compressed air is applied to the pressure chamber, the compressed air is evenly distributed to the capillaries and the fluid located in the capillaries is ejected.
  • capillaries are emptied in parallel via a common pressure supply.
  • Typical operating pressures are 15-25 psi and 1.0 - 1.7 bar, respectively.
  • capillary dispensers are therefore designed purely for the delivery of liquid into empty wells. However, this does not completely solve the problem of cross contamination.
  • the capillaries are never completely emptied immediately.
  • a film of liquid remains on the inside of the capillary, which moves slowly towards the end of the capillary by gravity and inflowing air. Here it is dusted due to the high flow velocity of the air to form an aerosol.
  • this aerosol can be detected as cross-contamination, due to the high concentration of active substance contained or with sufficiently sensitive measuring systems.
  • bubble wrap pipettes In the field of liquid handling systems, so-called bubble wrap pipettes are known that can pick up and deliver liquid into and out of an associated pipette tip using a piston-cylinder unit.
  • the pipette tip is in this case attached to an opening in an end face of the inner cylinder.
  • the piston is movably disposed within the inner cylinder on a common axis and sealed with respect to this.
  • the emptying of the capillary could, however, be carried out according to the principle of air displacement by decreasing the piston in the inner cylinder reduces the free cylinder volume and thus the air therein is pushed out through the capillary.
  • the air displacement could be very finely dosed. This allows the training of a Drop at the free end of the capillary, which can be selectively discontinued by contact with, for example, a slide.
  • the displaced by the piston free volume of the inner cylinder and thus the nachströmende from the capillary air was less than 50 ul in experiments and is thus by a factor of 20 to 200 less than with a capillary dispenser in which all capillaries are in communication with a pressure chamber.
  • This lower volume of air dispensed, with lower pressure and thus lower delivery rate allows both the dispensing on smooth surfaces and in vessels already containing a liquid, such as wells of a microtiter plate. An aerosol formation is thereby avoided as far as possible.
  • the piston After each delivery, the piston must be returned to its original position before re-dispensing, avoiding unwanted fluid intake.
  • the capillary dispenser should advantageously be modifiable with only minor structural changes in order to optimize it either for a non-contact delivery in liquids or a delivery under contact of the capillary.
  • the capillaries should be quickly exchangeable at a high repetition rate. This object is achieved for a capillary dispenser with the features of claim 1. Advantageous embodiments are described in the subclaims.
  • a capillary dispenser according to the invention can be equipped with only one capillary, several capillaries in a row or several capillaries in a matrix.
  • the capillaries can each be assigned to a piston-cylinder unit or a plurality of capillaries are connected to a common cylinder and thus associated with a common piston-cylinder unit 17.
  • a capillary dispenser Since a capillary dispenser is basically installed so that it dispenses in the direction of gravitational force, the following explanations are given as “above” or “below” clearly. They should also be understood as if a capillary dispenser is not operated in a vertical direction, for which there are applications especially for capillary dispensers with only one capillary.
  • a capillary dispenser has at least one piston-cylinder unit 17, which consists of a cylinder assembly 16 with an inner cylinder 1.1 and a piston assembly 15, with a piston 5.
  • the inner cylinder 1.1 and the piston 5 are arranged coaxially with one another on an axis A, wherein the piston 5 sealed relative to the inner cylinder 1.1, within this, along the axis A is movable by a stroke.
  • the movement can in principle be initiated manually, but, in particular, if the capillary dispenser has a larger number of capillaries 8, motorized, including the capillary dispenser having a drive and a controller.
  • the piston 5 is a hollow piston, which is opened at the beginning of its movement to the upper end position of the piston assembly 15 and then remains open on both sides over the length of the stroke.
  • the inner cylinder 1.1 is pneumatically connected to at least one capillary 8.
  • the pressure conditions change at the associated with the inner cylinder 1.1 end of the at least one capillary 8, while the piston 5 is moved to the upper end position of the piston assembly 15 out.
  • This movement thus has no influence on the voltage applied to the capillary 8 pressure conditions. That is, the capillary 8 could already be filled or at the same time, provided that it is in contact with a liquid with its other end (referred to below as the free end), it is filled exclusively via the capillary effect.
  • the piston-cylinder unit 17 thus constitutes a closed system.
  • the air in the piston-cylinder unit 17 is compressed air, bringing the Pressure at the associated with the inner cylinder 1.1 end of at least one capillary 8 steadily increased, while the piston 5 is moved to the lower end position of the piston assembly 15 out.
  • the air escapes via the at least one capillary 8, with which a liquid located therein is blown out.
  • the piston 5 Since the piston 5 must be opened after each emptying of the capillary 8 (venting), that is, the piston assembly 15 must be moved at least a small part of the stroke in the direction of the upper end position of the piston assembly 15, it is advantageous if the piston 5 and the Inner cylinder 1.1 are dimensioned over their length and their cross-section so that the stroke just enough for emptying the capillary 8. Thus, in each case with the emptying and venting of the full stroke is exhausted and the inner cylinder and the piston 1 1 can be dimensioned as small as possible.
  • the dimensioning can also be carried out in such a way that a stroke is sufficient to repeatedly empty the capillary 8.
  • the piston assembly 15 is then raised only slightly in the direction of the upper end position of the piston assembly 15 so that the capillary 8 opens.
  • the liquid in the inner cylinder 1.1 does not rise abruptly and is also relatively low, the liquid can also be dispensed into a vessel in which there is already a liquid, without resulting in a so-called crosstalk, as described in the description of Prior art has been described. Pulse-like delivery is possible if a valve 14 is provided in the connection between the inner cylinder 1.1 and the capillary 8.
  • the closure preferably takes place by means of a piston seal.
  • Either the piston 5 is formed directly with an end face formed on the upper end, the complainthast is rounded, applied to the piston seal, or the seal is made indirectly by a provided around the end of the piston 5, sealed to the circumference of the piston head 5.1 to the piston seal is created.
  • the latter has the advantage that on the dimensions of the piston head 5.1 a wider contact surface can be created.
  • the piston seal may be preferred as a piston sealing plate 6, made of an elastic material, for. As an elastomer, executed.
  • the sealing of all pistons 5 is then advantageously carried out with a same piston sealing plate 6. Instead, as a piston seal and individual cones or balls 12 are used. These cones or balls 12 may, for. As glass, ceramic or also preferably be made of an elastic material.
  • An advantageous embodiment which best meets the requirements for the precision and speed of dispensing of smallest volumes is a capillary dispenser with a multiplicity of capillaries 8 arranged in a matrix corresponding to the wells of commercially available microtiter plates, and one capillary per capillary 8 Cylinder unit 17 is present.
  • Such a capillary dispenser is intended in a first embodiment with reference to the Fig. 1-3 be explained.
  • the in Fig. 1a shown capillary dispenser with a matrix-like arrangement of capillaries 8 has a support plate 1, a pressure plate 2 and a piston retaining plate 3 and an adjacent to the support plate 1 indirectly via a sealing mat 10 magazine 9, in which the capillary 7 with the capillaries 8 hanging, non-positively the sealing mat 10 abut.
  • a piston-cylinder unit 17 is provided, which is arranged in each case on an axis A.
  • Fig. 1 For example, one of the axes A has been drawn.
  • a piston-sealing plate covering this 6 and an overlying stop plate 4 which are also assigned to the piston-cylinder units 17, in Fig. 1a not shown.
  • the recesses shown in the support plate 1 are provided for - necessary for automatic operation assemblies, such as control assembly - engine and transmission.
  • the support plate 1 at the same time forms the underside of a housing, not shown here.
  • piston-cylinder units 17 are shown as sectional views in the Fig. 2 to 5 to see.
  • a first embodiment of a piston-cylinder unit 17 is in the Fig. 3a and 3b shown, wherein the piston assembly 15 in 3a in an upper end position and in Fig. 3b is shown in a lower end position.
  • the piston-cylinder unit 17 essentially comprises an inner cylinder 1.1 and a concentric therewith arranged on a common axis A with the inner cylinder 1.1 piston 5. Also arranged on the axis A, is a capillary 8, the pneumatic with the inner cylinder 1.1 Connection stands.
  • the piston 5 is a hollow piston open on both sides, which can be closed on one side.
  • the inner cylinder 1.1 of a piston-cylinder unit 17 is formed by a through hole in the support plate 1. As shown, it may be a stepped bore comprising a larger inner diameter portion in which the piston 5 travels and a smaller diameter portion to form a channel whose diameter is greater than the diameter of the capillary 8 but smaller the diameter of a capillary holder 7 formed on the top collar is 7.2.
  • a piston 5 tightly enclosing sealing sleeve 11 is provided between the pressure plate 2 and the support plate 1.
  • the piston 5 of a piston-cylinder unit 17 is a hollow piston at the upper end of a piston head 5.1 is attached or formed.
  • the piston head 5.1 encloses the upper end of the piston 5 as a cuff and has two perpendicular to the axis A extending end surfaces 5.1.1 and 5.1.2.
  • On the lower face 5.1.1 of the piston head 5.1 can rest in a recess 3.1 of the piston retaining plate 3, which is deeper than the height of the piston head 5.1.
  • the piston head has 5.1 in the direction of the axis A an axial clearance equal to the difference between the height of the piston head 5.1 and the depth of the recess 3.1.
  • the upper end face 5.1.2 projects beyond the upper end of the piston 5, whereby it can be applied to the executed as a piston sealing plate 6 piston seal.
  • the upper end of the piston 5 can protrude beyond the upper end face 5.1.2, whereby the upper end of the piston 5 can be applied to the piston sealing plate 6.
  • Fig. 1a the top of the piston retaining plate 3 a plurality of recesses 3.1 in a same matrix-like grid as the capillaries 8.
  • the arranged in a row recesses 3.1 are connected via grooves 3.2 in conjunction, which together with the overlying, as a piston sealing plate. 6 formed piston seal form air ducts.
  • a stop plate 4 is arranged, which is fixed indirectly via the piston sealing plate 6 on the piston retaining plate 3 via detachable connections.
  • the piston retaining plate 3 and the stopper plate 4 are movable together along the axis A relative to the fixed support plate 1, whereby the piston 5 can be raised and lowered in the inner cylinder 1.1 according to the length of the stroke.
  • the piston retaining plate 3, the piston sealing plate 6, the stop plate 4 and the piston 5 with the respectively provided on it piston head 5.1 together form an equal number of piston assemblies 15, as capillaries 8 are present.
  • the capillary 8 assigned to a piston-cylinder unit 17 is pneumatically connected to the inner cylinder 1.1.
  • any type of connection is possible here, which represents a connection sealing off from the environment.
  • the capillaries 8 are introduced into the magazine 9 with the corresponding grid spacing and the magazine 9 is indirectly frictionally engaged on the carrier plate 1 via the sealing mat 10.
  • the term magazine 9 is intended to stand for a plane plate, in which the capillaries 8 are arranged, regardless of how they are held in the plate and whether they are releasably or not releasably secured in the plate or held unattached.
  • the capillaries 8 z. B. be embedded in a magazine 9.
  • the disadvantage here is that damaged, broken or clogged capillaries 8 can not be exchanged, which possibly makes the replacement of the entire magazine 9 required.
  • the capillaries 8 may instead individually in shafts, z. B. be taken from plastic and be releasably fixed in a magazine 9 via a provided on the shaft releasable screw, clamp or plug connection.
  • the apparent advantage of the aforementioned solution is that the capillaries 8 are individually and easily replaced with shanks which are positively and / or non-positively connected to the magazine 9.
  • the disadvantage is that such compounds can not be solved as often without wear and the tightening force to attach the Connection, may neither be too big nor too small, on the one hand not to damage the shaft and on the other hand to ensure a tight fit.
  • a capillary holder 7 which is designed like a pipette tip and into which Fig. 3a and 3b is shown. It consists of a tubular tip portion 7.1 and formed on this in the dispensing rear end tip collar 7.2 with a front and a rear, the tip portion 7.1 facing end.
  • the capillary holders 7 can be inserted with their tip portion 7.1 in designated holes in a magazine 9 until the rear end of the top collar 7.2 comes to rest on the magazine 9 and the capillary holder 7 with a minimal radial play in the magazine 9 depends.
  • the capillary 8 is at the front end of the capillary holder 7, at least slightly protruding from this, attached. You can z. B. glued or pressed.
  • the Kapillarhalterung 7 can be advantageously prepared as a monolithic injection molded part with the capillary 8 as an insert.
  • the magazine 9 can be positioned and fastened on the carrier plate 1 in such a way that the front end sides of the tip collars 7.2 are frictionally applied to the sealing mat 10.
  • the capillaries 8 are preferably made of glass, but may also consist of other materials such as plastic or ceramic, wherein it must be ensured that the capillary rise height of the liquid to be dispensed sufficient to completely fill the capillary 8.
  • the capillaries 8 preferably have a hydrophobic outer coating to prevent adhesion of the liquid when lifted out of the liquid as much as possible and to suppress the creeping of liquid on the outside of the capillary 8 in the formation of a drop at the capillary.
  • Particularly advantageous is a coating of fluoropolymer.
  • the capillaries 8, and their free ends as thin as possible to minimize the end face at the end of the capillary and thereby minimize the adhesion of liquid to the end face.
  • the piston assembly 15 is in an upper end position, as in Fig. 3a shown.
  • the piston head 5.1 is located at the bottom of the recess 3.1 in the piston retaining plate 3.
  • the piston 5 whose lower end is located in the inner cylinder 1.1, while the upper end of the air channels is in communication with the atmosphere, therefore prevails in the inner cylinder 1.1, the ambient pressure.
  • the capillary 8 automatically fills by its capillary action on the immersion of its free end, as long as the other end is open and a substantially same ambient pressure is applied as at the free end, the capillary 8 may already be filled or now by immersion in a liquid be filled.
  • a vessel filled with the liquid is preferably raised toward the capillaries 8.
  • a pressure For dispensing, a pressure must be built up in the inner cylinder 1.1, which presupposes that the inner cylinder 1.1 is closed and the free volume in it is subsequently reduced in order to expel the liquid from the capillary 8.
  • the closing of the inner cylinder 1.1 takes place indirectly via the one-sided closing of the piston 5 by the piston assembly 15 is lowered.
  • the piston head 5.1 is pressed indirectly via the piston sealing plate 6 to the stop plate 4 and closed.
  • the piston-cylinder unit 17 is thus sealed from the environment and it is built on a further lowering of the piston assembly 15 and thus the piston 5, a pressure for dispensing the liquid from the capillaries 8 until it is sufficient to expel the liquid.
  • a lower end position of the piston assembly 15 is given by the abutment of the piston retaining plate 3 on the pressure plate 2, but can also be defined by additionally existing stops.
  • the piston 5 is brought back into its upper end position by raising the piston assembly 15, with the piston 5 reopening so that the ambient pressure in the inner cylinder 1.1 is restored.
  • liquid can be taken up again via the capillary 8, wherein the receptacle is not effected by suction, but exclusively by the capillary effect.
  • the procedure described is synchronous for all piston-cylinder units 17 of the capillary dispenser.
  • the capillary dispenser should have an integer multiple of capillaries 8 than piston-cylinder units 17, as described in the first exemplary embodiment.
  • a number of capillaries 8 are connected to each inner cylinder 1.1, which corresponds to the multiple of the piston-cylinder units 17. With increasing number of capillaries 8 to be emptied via a piston-cylinder unit 17, a larger pressure in the inner cylinder 1.1 must be established.
  • piston sealing plate 6 instead of a piston sealing plate 6 as a piston seal for all piston-cylinder units 17, can also be per piston-cylinder unit 17 z.
  • a ball 12 are used to seal the piston 5, which is inserted in an additional auxiliary plate 13 in a recess 13.1.
  • a section of a piston assembly 15 is shown with a ball 12 as a piston seal in an upper and lower end position of the piston assembly 15.
  • the piston head is 5.1 in the lower end position of the piston head assembly 15 in the recess 3.1 on the bottom and the piston 5 is open.
  • the upper end of the piston 5 is applied directly to the ball 12 and the piston 5 is closed.
  • the ball 12 may be made of an elastomer or of glass, metal or ceramic with a high-precision surface.
  • the upper end of the piston 5 is made with an end face having a radius of curvature equal to the ball radius.
  • the balls 12 are each placed above the individual pistons 5 in an auxiliary plate 13 in recesses 13.1 provided for this purpose. Also in this embodiment 3 grooves 3.2 are provided in the piston retaining plate. You can be introduced accordingly in the adjacent side of the auxiliary plate 13.
  • a valve 14 may be attached to the lower end of the inner cylinder 1.1. With the aid of the valve 14, it is possible in the inner cylinder 1.1 by means of the piston 5 to build up the required pressure for dispensing and then release it as a pressure pulse.
  • miniature electromagnetic valves but also passive ball check valves and so-called Durckbills or cross-slit valves made of an elastomer come into question.
  • the problem with passive valves is that they typically do not release the pressure as a pulse but slowly open when a threshold is reached.

Claims (9)

  1. Un distributeur capillaire ayant au moins une unité piston-cylindre (17) composée d'un ensemble de cylindre (16) avec un cylindre intérieur (1.1) et d'un ensemble de piston (15) avec un piston (5) qui sont disposés ensemble sur un axe (A), le cylindre intérieur (1.1) étant relié de manière pneumatique à au moins un capillaire (8), le piston (5) étant scellé par rapport au cylindre intérieur (1.1) et étant configuré comme un piston creux et ouvert sur les deux côtés et étant déplaçable dans le cylindre intérieur (1.1) le long de l'axe (A) entre une position finale supérieure et une position finale inférieure de l'ensemble de piston (15), et le piston (5) étant fermable d'une côté pour fermer le piston (5) d'une côté pendant le mouvement vers la position finale inférieure de l'ensemble de piston (15).
  2. Le distributeur capillaire selon la revendication 1, caractérisé en ce que l'au moins un ensemble de piston (15) est associé à un joint de piston contre lequel une face supérieure du piston (5) est placé par friction, quand l'ensemble de piston (15) est dans la position finale supérieure, de sorte que le piston (5) est fermé, le piston (5) ayant un jeu axial dans la direction de l'axe (A) dans l'ensemble de piston (15).
  3. Le distributeur capillaire selon la revendication 1, caractérisé en ce que l'au moins un ensemble de piston (15) est associé à un joint de piston, et le piston (5) a une tête de piston (5.1) qui dépasse une face supérieure du piston (5) et qui est placée contre le joint de piston par friction, quand l'ensemble de piston (15) est dans la position finale supérieure, de sorte que le piston (5) est fermé, le piston (5) ayant un jeu axial dans la direction de l'axe (A) dans l'ensemble de piston (15).
  4. Le distributeur capillaire selon la revendication 2 ou 3, caractérisé en ce que le joint de piston est une sphère (12) et la face supérieure du piston (5) est configurée avec un rayon de courbure qui est égal au rayon de la sphère (12).
  5. Le distributeur capillaire selon la revendication 2 ou 3, caractérisé en ce que le joint de piston est une plaque d'étanchéité de piston (6).
  6. Le distributeur capillaire selon la revendication 1, caractérisé en ce que le cylindre intérieur (1.1) communique avec le capillaire (8) via une valve (14).
  7. Le distributeur capillaire selon la revendication 1, caractérisé en ce qu'au moins un capillaire (8) est inséré dans un porte-capillaire (7) qui a la forme d'une pointe de pipette.
  8. Le distributeur capillaire selon la revendication 1, caractérisé en ce que le distributeur capillaire comprend une pluralité de capillaires (8) et un porte-capillaire (7) sous la forme d'une pointe de pipette est présent par capillaire (8), le capillaire (8) étant inséré de manière fixe dans le porte-capillaire (7) avec une extrémité libre dépassant, et les portes-capillaire (7) sont suspendus dans une cartouche (9).
  9. Le distributeur capillaire selon la revendication 1, caractérisé en ce que chaque cylindre intérieur (1.1) communique avec plusieurs capillaires (8).
EP20130150872 2012-02-01 2013-01-10 Distributeur capillaire Active EP2623203B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE201210100836 DE102012100836B3 (de) 2012-02-01 2012-02-01 Kapillardispenser

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Publication Number Publication Date
EP2623203A1 EP2623203A1 (fr) 2013-08-07
EP2623203B1 true EP2623203B1 (fr) 2015-03-18

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CN (1) CN103240136B (fr)
DE (1) DE102012100836B3 (fr)

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CN109990860B (zh) * 2019-04-18 2024-03-19 山东省科学院海洋仪器仪表研究所 气动毛细管夹持装置
US11389792B2 (en) * 2019-10-25 2022-07-19 Mettler-Toledo Rainin, LLC Syringe for powered positive displacement pipette
CN113135306B (zh) * 2021-04-30 2022-07-08 杭州博日科技股份有限公司 磁珠与试剂自动灌装装置及方法
CN113135328B (zh) * 2021-04-30 2022-11-25 杭州博日科技股份有限公司 液体全自动分装流水线

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CN103240136A (zh) 2013-08-14
CN103240136B (zh) 2016-06-22
EP2623203A1 (fr) 2013-08-07

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