EP2983826B1 - Dispositif de pipette comportant une unite de microdosage - Google Patents

Dispositif de pipette comportant une unite de microdosage Download PDF

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Publication number
EP2983826B1
EP2983826B1 EP14726510.2A EP14726510A EP2983826B1 EP 2983826 B1 EP2983826 B1 EP 2983826B1 EP 14726510 A EP14726510 A EP 14726510A EP 2983826 B1 EP2983826 B1 EP 2983826B1
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EP
European Patent Office
Prior art keywords
pipette
unit
liquid
pipette tip
micro
Prior art date
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Active
Application number
EP14726510.2A
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German (de)
English (en)
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EP2983826A1 (fr
Inventor
Steffen Gehrig
Burkard Schaub
Jürgen Schraut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brand GmbH and Co KG
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Brand GmbH and Co KG
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Publication date
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Publication of EP2983826A1 publication Critical patent/EP2983826A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237—Details of electronic control, e.g. relating to user interface
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Definitions

  • the invention relates to a pipetting device with a microdosing unit having the features of the preamble of claim 1 and of claim 9, respectively.
  • a pipetting device of the type in question may in principle have only a single pipette tip at a coupling point.
  • such a pipetting device is a multi-channel pipetting device, in which therefore the pipetting unit has a plurality of coupling points and a corresponding number of pipette tips which can be attached to these coupling points.
  • such a multi-channel pipetting device is used on a pipetting or pipetting robot.
  • the pipetting unit is brought into different operating positions by means of a positioning device.
  • the positioning device may be an X / Y / Z traversing device. It can also be the arm of a robot, which can be moved largely freely in space.
  • the prior art gives various suggestions.
  • the pipette tips are received at a receiving position from a peak supply, that is coupled to the coupling points. Then, the pipetting unit is moved by the positioning in a liquid-receiving position. For receiving the liquid into the pipette tips, ie for aspiration, the mouth openings of the pipette tips are immersed in the liquid in corresponding vessels, for example the wells of a microtiter plate. The liquid is absorbed by the action of the displacer unit or displacer units in the pipette unit.
  • the pipetting unit is moved by means of the positioning device into a liquid dispensing position.
  • the desired volume of liquid dispensed from the respective pipette tip into a target vessel may again be wells of a microtiter plate.
  • the liquid delivery can be done with larger volumes as free-flying jet. For small volumes, it may even be necessary to make contact between the target vessel and the pipette tips so that the adhesion force of the liquid drop at the pipette tip can be overcome.
  • the aforementioned boundary conditions limit the deliverable liquid volume down to volumes in the range of a few microliters. Dispensing dosages in the nanoliter range can not be realized this way.
  • the classical pipetting machine is at risk of carryover of substances already in the target vessel.
  • the pipetting unit is brought by means of the positioning device in an ejection position, in which then by means of the displacer unit, the remaining liquid is ejected in the pipette tips.
  • the pipette unit is brought into a pipette tip dropping position. There, the pipette tips are released by means of a tip ejection device from the coupling points on the pipetting unit and dropped into a collecting container.
  • the microdosing unit of the pipetting device is an arrangement with clamping jaws as an abutment for the movable actuating device, which itself is driven piezoelectrically.
  • this is also on the WO 2005/016534 A1 for such a microdosing unit, which discloses details of the structure of such a microdosing unit.
  • the microdosing unit of the pipetting device is stationary at the liquid dispensing position.
  • the pipette tip must be threaded into the actuator of the microdosage unit from above.
  • the liquid within the pipette tip is tracked into the tube by capillary forces. Purging liquid in small portions from a pipette tip into a plurality of different target vessels requires movement of the target vessels relative to the pipetting tip stationary in the liquid dispensing position.
  • the pipetting device described above is theoretically suitable for dispensing smallest volumes at various locations, but still has to optimize the pipetting device in practical operation.
  • the invention is based on the problem of specifying a practical designed pipetting device with a microdosing unit.
  • the microdosing unit is designed as a separate unit from the pipetting unit.
  • the pipetting unit in various relevant operating positions, in particular in the pipette tip receiving position, the liquid receiving position, but also preferably in the pipette tip dropping position and optionally the discharge position for residual liquid, such as a classic pipetting a pipetting without Microdosierü be handled and used can.
  • the Microdosing unit coupled to the pipetting unit, in a well-defined relative position to the pipetting unit when it is needed, namely in the liquid dispensing position.
  • the microdosing is coupled to the pipette unit and can then be brought together with the pipette by means of the positioning in different operating positions, for example, several different liquid dispensing positions.
  • the pipetting device has a pipetting unit without a microdosing unit where the microdosing unit is not needed. It converts to a pipetting unit with a microdosage unit where necessary, namely when dispensing liquid.
  • the pipetting unit together with its microdosing unit can be moved freely, for example via different microtiter plates which are located at different locations and which in turn do not have to be moved.
  • the pipetting device according to the invention can thus also be used for different volumes.
  • the pipetting device can be operated without a microdosing unit, optionally also with normal pipette tips without elastically deformable ejection ends. If you want to dose in the range of very small volumes, the microdosing is coupled as needed to the pipette and can then be brought with this to the desired operating position.
  • Typical materials for an elastically deformable discharge end of the pipette tip are polyimide, polyamide or silicone. Typical diameters are 0.1 to 1 mm.
  • the elastically deformable ejection end may be integrally formed on the pipette tip. It can also be attached by gluing, injecting, shrinking or pressing on the pipette tip by the way.
  • the cross section of the elastically deformable ejection end may have any shape, but will generally be round.
  • the displacer unit of the pipette is controlled in accordance with the dispensing of the liquid by means of the microdosing so that the dispensed minimum volume of liquid is fed directly into the discharge end of the pipette tip.
  • the pipette tip in the ejection end approximately always the same pressure. This allows a high precision in the liquid delivery with the help of the microdosing in several stages or steps.
  • the pipette tip is positioned relative to an actuating device of the microdosing unit such that a lower section of the pipette tip immediately above the outlet opening always remains underneath the actuating device and does not touch it.
  • the displacing means may expel liquid from the pipette tip so that the meniscus of the liquid is formed at the mouth of the pipette tip.
  • the microdosing unit is put into operation between method steps d) and e), with its actuating device acting in a reciprocating motion on a tube located at the lower end of the pipette tip, which is arranged in front of the mouth opening, such that this is deformed radially elastic and volume reducing so that the liquid is discharged in the tube in the form of free-flying droplets or in the form of a free-flying jet.
  • the coupling and decoupling of the microdosing to the pipetting in the context of the preferred method may preferably be done by switched coupling devices, preferably a coupling device is a magnetic coupling, more preferably a coupling device with a current-magnet permanent magnet / electromagnet.
  • a residual liquid in the pipette tip by the displacer unit ejected which preferably takes place in a separate liquid dispensing position.
  • the displacer unit will feed this liquid volume as an overpressure directly or discontinuously.
  • liquid of smallest volumes, volumes up to 0.1 nl, held by a pipetting unit pipette tip can be transferred from a storage vessel to one or more target vessels with the inventive method.
  • the pipette tip has a tube arranged in front of its mouth opening.
  • the coupling of the pipette tip to a pipetting unit of the pipetting device, the picking up of the liquid, possibly the dispensing of residual liquid and the ejection of the pipette tip take place as in conventional pipetting devices.
  • the dispensing of the liquid in very small volumes takes place by means of a microdosing unit, which is switchably connected to the pipetting unit after the liquid has been taken up and later decoupled again.
  • the pipetting unit is moved by means of the positioning device to a coupling position and there the pipette tip is moved with its tube between an abutment and an actuating device of the micro-dosing unit.
  • the abutment is attached to the tube.
  • the pipetting unit with its coupling device is also coupled to the counter coupling device of the microdosing unit and, preferably, in the coupling position via the pipetting unit, the microdosing unit is connected to the control device in terms of control technology.
  • the pipetting unit is then moved with the microdosing unit by means of the positioning device to one or more liquid dispensing positions, so that the mouth opening of the corresponding pipette tip is arranged above a destination vessel, and there the microdosing unit for droplet-like liquid dispensing is put into operation.
  • the pipetting unit is then moved back to the coupling position by means of the positioning device and there the coupling device is uncoupled from the counter coupling device, the abutment moved away from the tube in a starting position and the microdosing off again.
  • Fig. 1 shows a pipetting with a pipette 1.
  • At the pipette 1 at least one pipette tip 2 is replaceable attachable.
  • the pipetting device is a multi-channel pipetting device. Then there are several pipette tips 2 at the pipetting unit 1.
  • the pipetting device according to the invention is one having a pipette tip 2, which has an elastically deformable ejection end 3.
  • the elastically deformable discharge end 3 has the shape of an elastically deformable tube in the illustrated embodiment.
  • Such a tube may have any cross section, for example, an elliptical cross section.
  • a cross section for example, an elliptical cross section.
  • the pipetting device has a positioning device 4 and a microdosing unit 5.
  • the entire pipetting device is completed by an electrical, electronic, often software-controlled control device 6, by which all processes of the pipetting device can be controlled as desired.
  • Fig. 1 is indicated by dashed lines where transmission means for control commands, power supply and / or data are provided between the individual components of the pipetting device.
  • the pipetting unit 1 has a coupling point 7 for each attachable pipette tip 2.
  • This coupling is typically designed in the form of a pipette shaft.
  • the pipette tip 2 is plugged onto the coupling point 7 formed by the pipette shaft or the coupling point 7 is inserted into the open upper end of the pipette tip 2 in order to couple the pipette tip 2 stored in a holder or support frame 16.
  • the pipette unit 1 has a displacer unit 8 fluidically connected to the coupling point 7 and the pipette tip 2 attached thereto.
  • the displacer unit 8 is typically a cylinder-piston arrangement. It is essential that the displacer unit 8 in the coupled pipette tip 2 can generate a negative pressure for taking up liquid in the pipette tip 2 and an overpressure for ejecting liquid from the pipette tip 2. Also, reference may be made to the entire state of the art explained in the introduction.
  • the pipetting unit 1 can be brought into different operating positions.
  • Typical operating positions are a pipette tip pickup position ( Fig. 2 ), a liquid receiving position ( Fig. 3 ), a coupling position ( Fig. 4 ), a liquid dispensing position ( Fig. 5 ) or a plurality of such liquid dispensing positions, and a pipette tip ejection position ( Fig. 6 ).
  • residual liquid ejection position In between can also be a residual liquid ejection position, which is not shown in the drawing.
  • residual liquid ejection position of the pipette unit 1 residual liquid from the pipette tips 2 may be discarded before being dropped into a container at the pipette tip ejection position.
  • microdosing unit 5 is controlled by its own control unit 15.
  • the microdosing unit 5 In order to interact with the elastically deformable discharge end 3, the elastically deformable tube realized here directly above the mouth opening of the pipette tip 2, the microdosing unit 5 has a movable actuating device 9.
  • the microdosing unit 5 is, as already Fig. 1 can be seen in the dispensing position of the pipette 1 relative to the pipette tip 2 arranged so that the actuator 9 is positioned on the elastically deformable discharge end 3 of the pipette tip 2.
  • dispensing position of the pipette 1 is by driving the microdosing 5, the discharge end 3 of the pipette tip 2 by means of the actuator 9 radially elastically deformable volume displacing.
  • one or more liquid can be ejected from the discharge end 3 of the pipette tip 2 as the smallest droplets or as a free-flowing jet.
  • ejecting the liquid in the smallest volumes is not effected by the displacer unit 8, but by the microdosing unit 5 whose actuating device 9 influences the ejection end 3 of the pipette tip 2 directly in such a way that the volume in the ejection end 3 decreases.
  • the ejection end 3 is thus deformed by means of the actuating device 9, abruptly with a high pulse, so that the liquid is ejected in the form of minute droplets or as a short free-flying beam. Smallest amounts of liquid down to 0.1 nl can be ejected.
  • Fig. 2 to 7 the construction of the microdosing unit 5 with respect to the pipetting unit 1 is shown. It is provided that the microdosing unit 5 is executed separately from the pipetting unit 1 but can be coupled with the pipetting unit 1 in a precisely defined relative position, that the pipetting unit 1 has a coupling device 10 for this purpose and the microdosing unit 5 has a corresponding counter coupling device 11 that the coupling device 10 and / or the counter-coupling device 11 is switchable and that by switching the coupling device 10 and / or the negative feedback device 11, the micro-dosing unit 5 can be coupled to the pipetting unit 1 or decoupled from the pipetting unit 1.
  • the microdosing unit 5 therefore does not always have to be coupled to the pipetting unit 1. It is only necessary to couple with the pipetting unit 1 if, in the liquid dispensing position with the microdosing unit 5, smallest amounts of liquid are to be dispensed into target vessels.
  • the pipetting device has a storage platform 12 for storing the microdosing unit 5 when not in use.
  • the storage platform 12 provides the deliberately provided, defined space for storage of the microdosing unit 5 when not in use. It is expediently positioned in a fixed position throughout the pipetting device. Not in Fig. 4 A positioning aid is shown between the pipetting device or the deposition platform 12 on the one hand and the microdosing unit 5 on the other hand for exact and positionally stable positioning of the microdosing unit 5.
  • the coupling device 10 and the negative feedback device 11 there are in principle a variety of possibilities.
  • the coupling device 10 and the counter coupling device 11 it would be possible for the coupling device 10 and the counter coupling device 11 to be designed mechanically, ie as a tongue / groove connection or as a bolt connection, etc.
  • the negative feedback device 11 is active and switchable by the control device 6 or the control unit 15 and the coupling device 10 is made passive.
  • a switched magnetic coupling can generate a coupling effect when a solenoid is energized.
  • the electromagnet would have during the entire process with the micro-dosing unit 5 at the Pipetting 1 are energized.
  • the associated power consumption would be comparatively high.
  • the invention in the negative feedback device 11 is constructively worked with at least one permanent magnet 13 and an electromagnet 14 associated with the permanent magnet 13.
  • the electromagnet 14 When the electromagnet 14 is not energized, the holding force of the permanent magnet 13 causes the coupling of the microdosing unit 5 to the pipetting unit 6.
  • the coupling device 10 has a simple counterpart 10 'of ferromagnetic material.
  • the electromagnet 14 If the electromagnet 14 is energized with current in one direction, which leads to a magnetic field of the permanent magnet 13 opposite, but approximately the same size magnetic field, the magnetic force of the permanent magnet 13 is neutralized.
  • the electromagnet 14 is energized, a decoupling of the negative feedback device 11 from the coupling device 10 and thus the microdosing unit 5 is effected by the pipetting unit 1.
  • This selective energization of the electromagnet 14 can be realized, for example, when sections act on high accelerations on the microdosing 5 or otherwise the microdosing 5 is exposed to special forces.
  • Another advantage of the present design of the preferred embodiment is that with neutralized magnetic force of the permanent magnet 13, the pipetting unit 1 can be positioned freely and easily relative to the microdosing unit 5 by means of the positioning device 4. Only when the defined relative position is reached, which can be achieved, for example, by corresponding positioning aids, the permanent magnets 13 are released with respect to their magnetic effect.
  • the illustrated and preferred embodiment shows a modern, combined element of permanent magnet 13 and electromagnet 14.
  • Such integrated elements are particularly compact and have a particularly high performance.
  • the microdosing unit 5 is connected in terms of control technology by coupling to the pipetting unit 1 with the control device 6 of the pipetting device.
  • the microdosing unit 5 may be autonomous in terms of control technology, wherein it communicates with the control device 6 of the pipetting device in a contact-bound or wireless manner.
  • an electrical contact connection between the pipetting device and microdosing unit 5 is established.
  • control signals or the voltage supply or charging currents for rechargeable batteries or capacitors of the microdosing unit 5 can be conducted via this contact connection.
  • the electrical contact connection can also be arranged between the pipetting unit 1 and microdosing unit 5 and connected to the coupling.
  • the microdosing unit 5 has its own control unit 15, which has already been mentioned above. Suggestions for this will also be described later in the discussion of the embodiment.
  • Fig. 3 you can see the situation in the pipette tip pickup position.
  • the pipette tip 2 is suspended in the support frame 16 of a storage container.
  • the displacer unit 8 in the form of a cylinder-piston arrangement is located together with the associated drive 17 on a holder 18.
  • the control device 6 of the pipetting device is indicated purely schematically on the drive 17. Of course, this can also be arranged at any other location.
  • Fig. 3 the pipette tip 2 has been attached to the coupling point 7.
  • the pipetting unit 1 has been brought into the liquid receiving position by means of the positioning device 4.
  • the piston of the displacer unit 8 can be moved a little further and suck some air, so that no liquid drips from the mouth of the pipette tip 2, when the pipetting unit 1 to another operating position is moved.
  • Fig. 4 shows a representation of the coupling position.
  • the pipetting unit 1 has been brought together with the microdosing unit 5.
  • the coupling device 10 on the pipetting unit 1 interacts with the negative feedback device 11 in such a way that the microdosing unit 5 is firmly coupled to the pipetting unit 1.
  • the actuating device 9 of the microdosing unit 5 shown must of course be located exactly where the discharge end 3 of the associated pipette tip 2 is located.
  • Left in Fig. 4 at the level of the discharge end 3 of the pipette tip 2 can be seen a jaw 20.
  • the arrow indicates that the jaw 20 is employed at the discharge end 3 of the pipette tip 2, so that there is an abutment for the movement of the actuator 9.
  • Fig. 5 shows the actual liquid delivery in the liquid dispensing position.
  • target vessels 21 ' for example the wells of a microtiter plate, and above the direction of movement of the pipetting unit 1 together with the microdosing unit 5, moved by the positioning device 4.
  • Shown is a deformation effect of the actuator 9 of the microdosing unit 5 from the discharge end 3 of the pipette tip 2 small liquid drop blasted, for example, 0.5 l volume.
  • the displacer unit 8 of the pipetting unit 1 can be controlled in accordance with the dispensing of liquid by means of the microdosing 5 so that the dispensed minimum volume of liquid after a number of liquid discharges is fed discontinuously or directly into the discharge end 3 of the pipette tip 2 into it.
  • the pressure in the air cushion in the pipette tip 2 is kept constant.
  • This process at the pipetting device according to the invention is also in Fig. 5 shown.
  • the microdosing unit 5 is first turned off again at the coupling position on its depositing platform 12, the clamping jaw 20 is moved away from the ejecting end 3 into the starting position and the coupling device 10; 11 solved.
  • the storage platform 12 for example, indicated in the microdosing unit 5 batteries 22 are automatically recharged.
  • a contact-bound electrical connection for the charging current and / or for a data exchange with the control device 6 is located between the microdosing unit 5 and the storage platform 12.
  • Fig. 6 the end position of the pipetting unit 1 is shown after a completed pipetting process.
  • the pipetting unit 1 is again by itself and has been brought as such by means of the positioning device 4 in the pipette tip dropping position.
  • the tip ejector 23 By means of the tip ejector 23, the pipette tip 2 is dropped into a receptacle. This collection container is not shown here.
  • Fig. 7 Details of a pipetting device with a plurality of pipette tips 2, namely four pipette tips 2, and with a plurality of displacement units 8, namely a cylinder-piston arrangement per coupling point 7 with pipette tip 2.
  • the piston rods of the cylinder-piston assemblies, the displacer unit form, are brought together on a common drive 17. Below you can see the jaws 20 and the target vessels 21st

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
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Claims (15)

  1. Dispositif de pipette avec
    une unité de pipette (1),
    au moins une pointe de pipette (2) à poser de façon remplaçable sur l'unité de pipette (1), avec une extrémité d'expulsion (3) élastiquement déformable,
    un dispositif de positionnement (4),
    une unité de microdosage (5), et
    un dispositif de commande (6),
    dans lequel l'unité de pipette (1) présente pour la ou chaque pointe de pipette à poser (2) une zone de couplage (7), en particulier en forme de tige de pipette,
    dans lequel l'unité de pipette (1) présente une unité de refoulement (8) reliée en écoulement à la zone de couplage (7) et à la pointe de pipette (2) posée sur celle-ci, en particulier sous la forme d'un agencement piston-cylindre,
    dans lequel l'unité de pipette (1) peut être amenée par le dispositif de positionnement (4) dans différentes positions, parmi lesquelles une position de délivrance de liquide,
    dans lequel l'unité de microdosage (5) est réalisée séparément de l'unité de pipette (1),
    dans lequel l'unité de microdosage (5) présente un dispositif d'actionnement mobile (9) et est en tout cas disposée dans la position de délivrance de l'unité de pipette (1) par rapport à la pointe de pipette (2), de telle manière que le dispositif d'actionnement (9) soit positionné à l'extrémité d'expulsion (3) de la pointe de pipette (2), et
    dans lequel dans la position de délivrance de l'unité de pipette (1) l'extrémité d'expulsion (3) de la pointe de pipette (2) peut être influencée au moyen du dispositif d'actionnement (9) par commande de l'unité de microdosage (5), de telle manière qu'un liquide puisse ainsi être expulsé en une ou plusieurs fois sous forme de très petites gouttes ou de jet libre hors de l'extrémité d'expulsion (3) de la pointe de pipette (2),
    caractérisé en ce que
    l'unité de microdosage (5) peut être couplée au choix à l'unité de pipette (1) dans une position relative exactement définie,
    l'unité de pipette (1) présente à cet effet un dispositif de couplage (10) et l'unité de microdosage (5) présente un dispositif de couplage opposé correspondant (11),
    le dispositif de couplage (10) et/ou le dispositif de couplage opposé (11) est commutable,
    par couplage du dispositif de couplage (10) et/ou du dispositif de couplage opposé (11) l'unité de microdosage (5) peut être au choix couplée à l'unité de pipette (1) ou découplée de l'unité de pipette (1), et l'unité de microdosage (5) couplée à l'unité de pipette (1) peut être amenée en même temps que l'unité de pipette (1) dans différentes positions, en particulier dans la position de délivrance de liquide.
  2. Dispositif de pipette selon la revendication 1, caractérisé en ce que l'extrémité d'expulsion (3) de la pointe de pipette (2) est réalisée en forme de petit tube élastiquement déformable et peut être déformée au moyen du dispositif d'actionnement (9) en refoulant un volume de façon radialement élastique.
  3. Dispositif de pipette selon la revendication 1 ou 2, caractérisé en ce qu'il est prévu une plate-forme de dépôt (12) pour le dépôt de l'unité de microdosage (5) lorsqu'elle n'est pas utilisée.
  4. Dispositif de pipette selon l'une quelconque des revendications 1 à 3, caractérisé en ce que le dispositif de couplage opposé (11) peut être couplé de façon active et le dispositif de couplage (10) peut être couplé de façon passive.
  5. Dispositif de pipette selon l'une quelconque des revendications 1 à 4, caractérisé en ce qu'un dispositif de couplage (10; 11) est réalisé en forme de couplage magnétique couplé, de préférence avec au moins un aimant permanent (13) et un électroaimant (14) associé à l'aimant permanent (13) et pouvant être alimenté en courant pour le couplage, et en ce que l'autre dispositif de couplage (10; 11) est réalisé sous forme passive, de préférence comme pièce opposée ferromagnétique (10'), dans lequel, lorsque l'électroaimant (14) n'est pas alimenté, la force de maintien de l'aimant permanent (13) assure le couplage de l'unité de microdosage (5).
  6. Dispositif de pipette selon l'une quelconque des revendications 1 à 5, caractérise en ce que l'unité de pipette (1) et/ou l'unité de microdosage (5) présent des accessoires de positionnement pour le respect exact de la position relative définie.
  7. Dispositif de pipette selon l'une quelconque des revendications 1 à 6, caractérisé en ce que l'unité de pipette présente une multiplicité de zones de couplage (7) pour la pose d'une multiplicité de pointes de pipette (2) (unité de pipette à canaux multiplies).
  8. Dispositif de pipette selon la revendication 7, caractérisé en ce qu'il est prévu une unité de refoulement (8) pour chaque zone de couplage (7) ou, de préférence, il est prévu une seule unité de refoulement (8) commune pour toutes les zones de couplage (7).
  9. Dispositif de pipette selon l'une quelconque des revendications 1 à 8, caractérise en ce que l'unité de refoulement (8) de l'unité de pipette (1) peut être commandée au moyen du dispositif de commande (6) en accord avec la délivrance de liquide au moyen de l'unité de microdosage (5), de telle manière que le volume de liquide minimal délivré soit immédiatement rechargé dans l'extrémité d'expulsion (3) de la pointe de pipette (2).
  10. Procécé pour transférer du liquide avec un dispositif de pipette selon la revendication 1, comportant les étapes de procédé suivantes:
    a) l'unité de pipette est déplacée au moyen du dispositif de positionnement à une position de pose de pointe de pipette et y est abaissée avec sa tige de pipette à l'avant verticalement vers une pointe de pipette stockée dans un support avec une extrémité d'expulsion élastiquement déformable, dans lequel la pointe de pipette est engagée en un point de couplage sur la tige de pipette.
    b) l'unité de pipette est déplacée au moyen du dispositif de positionnement à une position de réception de liquide et la pointe de pipette y est plongée avec son ouverture de sortie dans un récipient de stockage rempli de liquide et ici du liquide est reçu dans la pointe de pipette par l'action d'un dispositif de refoulement dans l'unité de pipette, qui est relié en écoulement à la pointe de pipette et qui peut produire une dépression et une surpression.
    c) l'unité de pipette est déplacée à une position de couplage au moyen du dispositif de positionnement.
    d) dans la position de couplage, l'unité de pipette est couplée à l'unité de microdosage.
    e) l'unité de pipette est déplacée en même temps que l'unité de microdosage au moyen du dispositif de positionnement à une ou plusieurs position(s) de délivrance de liquide, de telle manière que l'ouverture de sortie soit disposée au-dessus d'un récipient cible, et ici l'unité de microdosage est mise en marche pour la délivrance de liquide en gouttelettes, de préférence on la déplace dans plusieurs positions de délivrance de liquide et on répète la délivrance de liquide.
    f) l'unité de pipette est de nouveau déplacée au moyen du dispositif de positionnement à la position de couplage et l'unité de microdosage est ici de nouveau découplée de l'unité de pipette.
    g) l'unité de pipette est déplacée au moyen du dispositif de positionnement à une position d'éjection de la pointe de pipette et la pointe de pipette est ici éjectée dans un récipient de collecte au moyen du dispositif d'éjection de pointe.
  11. Procédé selon la revendication 10, caractérisé en ce que dans la caractéristique d), la pointe de pipette est positionnée par rapport à un dispositif d'actionnement de l'unité de microdosage de telle manière qu'une partie inférieure de la pointe de pipette, qui se trouve immédiatement au-dessus de l'ouverture de sortie, reste toujours en dessous du dispositif d'actionnement et ne le touche pas.
  12. Procécé selon la revendication 10 ou 11, caractérisé en ce que le dispositif de refoulement expulse du liquide hors de la pointe de pipette, entre les étapes de procédé c) et e), de telle manière que le ménisque du liquide se forme à l'ouverture de sortie de la pointe de pipette.
  13. Procédé selon l'une quelconque des revendications 10 à 12, caractérisé en ce que l'unité de microdosage est mise en marche entre les étapes de procédé d) et e), dans lequel son dispositif d'actionnement agit en un mouvement réciproque sur l'extrémité d'expulsion se trouvant à l'extrémité inférieure de la pointe de pipette, de telle manière que celle-ci soit déformée radialement de façon élastique et en diminuant de volume, de telle manière que le liquide se trouvant dans le petit tube soit délivré sous forme de gouttelettes volant librement ou sous forme de jet volant librement.
  14. Procédé selon l'une quelconque des revendications 10 à 13, caractérisé en ce qu'entre les étapes de procédé e) et f) un liquide résiduel dans la pointe de pipette est expulsé par l'unité de refoulement, dans lequel cela se produit de préférence dans une position de délivrance de liquide distincte.
  15. Procédé selon l'une quelconque des revendications 10 à 14, caractérisé en ce que pendant l'étape de procédé e) ou dès que l'unité de microdosage a été mise en marche pour la délivrance de liquide et qu'un volume de liquide est délivré hors de l'ouverture de sortie de la pointe de pipette, l'unité de refoulement recharge ce volume de liquide en suppression immédiatement ou de façon discontinue.
EP14726510.2A 2013-04-11 2014-04-10 Dispositif de pipette comportant une unite de microdosage Active EP2983826B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202013003390.8U DE202013003390U1 (de) 2013-04-11 2013-04-11 Pipettiervorrichtung mit einer Mikrodosiereinheit
PCT/EP2014/000965 WO2014166635A1 (fr) 2013-04-11 2014-04-10 Dispositif de pipette comportant une unite de microdosage

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EP2983826A1 EP2983826A1 (fr) 2016-02-17
EP2983826B1 true EP2983826B1 (fr) 2017-09-13

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EP (1) EP2983826B1 (fr)
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Publication number Publication date
US20160067705A1 (en) 2016-03-10
DE202013003390U1 (de) 2014-07-14
US10058859B2 (en) 2018-08-28
WO2014166635A1 (fr) 2014-10-16
EP2983826A1 (fr) 2016-02-17

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