EP2983826A1 - Dispositif de pipette comportant une unite de microdosage - Google Patents

Dispositif de pipette comportant une unite de microdosage

Info

Publication number
EP2983826A1
EP2983826A1 EP14726510.2A EP14726510A EP2983826A1 EP 2983826 A1 EP2983826 A1 EP 2983826A1 EP 14726510 A EP14726510 A EP 14726510A EP 2983826 A1 EP2983826 A1 EP 2983826A1
Authority
EP
European Patent Office
Prior art keywords
unit
pipetting
pipette tip
microdosing
pipette
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP14726510.2A
Other languages
German (de)
English (en)
Other versions
EP2983826B1 (fr
Inventor
Steffen Gehrig
Burkard Schaub
Jürgen Schraut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brand GmbH and Co KG
Original Assignee
Brand GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brand GmbH and Co KG filed Critical Brand GmbH and Co KG
Publication of EP2983826A1 publication Critical patent/EP2983826A1/fr
Application granted granted Critical
Publication of EP2983826B1 publication Critical patent/EP2983826B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237—Details of electronic control, e.g. relating to user interface
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Definitions

  • the invention relates to a pipetting device with a microdosing unit having the features of the preamble of claim 1 and of claim 9, respectively.
  • a pipetting device of the type in question may in principle have only a single pipette tip at a coupling point.
  • such a pipetting device is a multi-channel pipetting device, in which therefore the pipetting unit has a plurality of coupling points and a corresponding number of pipette tips which can be attached to these coupling points.
  • such a multi-channel pipetting device is used on a pipetting or pipetting robot.
  • the pipetting unit is brought into different operating positions by means of a positioning device.
  • the positioning device may be an X / Y / Z traversing device. It can also be the arm of a robot, which can be moved largely freely in space. The state of the art offers various suggestions for this.
  • the pipette tips are received at a receiving position from a peak supply, that is coupled to the coupling points. Then the pipetting unit is moved by means of the positioning device into a liquid receiving position. For receiving the liquid into the pipette tips, ie for aspiration, the mouth openings of the pipette tips are immersed in the liquid in corresponding vessels, for example the wells of a microtiter plate. The liquid is absorbed by the action of the displacer unit or displacer units in the pipette unit.
  • the pipetting unit is moved by means of the positioning device into a liquid dispensing position.
  • the desired volume of liquid is dispensed from the respective pipette tip into a destination vessel.
  • These may again be wells of a microtiter plate.
  • the liquid delivery can be done with larger volumes as free-flying jet. For small volumes, it may even be necessary to make contact between the target vessel and the pipette tips so that the adhesion force of the liquid drop at the pipette tip can be overcome.
  • the aforementioned boundary conditions limit the deliverable liquid volume down to volumes in the range of a few microliters. Dispensing dosages in the nanoliter range can not be realized in this way.
  • the classical pipettor is at risk of carryover of substances already in the target vessel.
  • the pipetting unit is brought by means of the positioning device in an ejection position, in which then by means of the displacer unit, the remaining liquid is ejected in the pipette tips.
  • the pipette unit is brought into a pipette tip dropping position. There, the pipette tips are released by means of a tip ejection device from the coupling points on the pipetting unit and dropped into a collecting container.
  • the microdosing unit of the pipetting device is an arrangement with clamping jaws as an abutment for the movable actuating device, which itself is driven piezoelectrically.
  • WO 2005/016534 A1 discloses details of the structure of such a microdosing unit.
  • the microdosing unit of the pipetting device is stationary at the liquid dispensing position.
  • the pipette tip must be threaded into the actuator of the microdosage unit from above.
  • the liquid within the pipette tip is tracked into the tube by capillary forces.
  • Low liquid ejection from a pipette tip into a plurality of different target vessels requires movement of the target vessels relative to the pipetting tip stationary in the liquid dispensing position.
  • the pipetting device described above is theoretically suitable for dispensing smallest volumes at various locations, but still has to optimize the pipetting device in practical operation.
  • the invention is based on the problem of specifying a practical designed pipetting device with a microdosing unit.
  • the microdosing unit is designed as a unit separate from the pipetting unit.
  • the pipetting unit is handled in various relevant operating positions, in particular in the pipette tip receiving position, the liquid receiving position, but also preferably in the pipette tip ejection position and possibly the ejection position for residual liquid, such as a classical pipetting unit of a pipetting device without microdosing unit can be used.
  • the Microdosing unit coupled to the pipetting unit, in a well-defined relative position to the pipetting unit when it is needed, namely in the liquid dispensing position.
  • the microdosing is coupled to the pipette unit and can then be brought together with the pipette by means of the positioning in different operating positions, for example, several different liquid dispensing positions.
  • the pipetting device has a pipetting unit without a microdosing unit where the microdosing unit is not needed. It converts to a pipetting unit with a microdosage unit where necessary, namely when dispensing liquid.
  • the pipetting unit together with its microdosing unit can be moved freely, for example via different microtiter plates which are located at different locations and which in turn do not have to be moved.
  • the pipetting device according to the invention can thus also be used for different volumes.
  • the pipetting device can be operated without a microdosing unit, optionally also with normal pipette tips without elastically deformable ejection ends. If you want to dose in the range of very small volumes, the microdosing is coupled as needed to the pipette and can then be brought with this to the desired operating position.
  • Typical materials for an elastically deformable discharge end of the pipette tip are polyimide, polyamide or silicone. Typical diameters are 0.1 to 1 mm.
  • the elastically deformable ejection end may be integrally formed on the pipette tip. It can also be attached by gluing, injecting, shrinking or pressing on the pipette tip by the way.
  • the cross section of the elastically deformable ejection end may have any shape, but will be round as a rule.
  • a method of transferring liquid with a pipetting device comprising a pipetting unit, a positioning device, a micro-dosing unit and a control device, comprising the following method steps:
  • the pipetting unit is moved by means of the positioning device to a pipette tip receiving position, where it is lowered with its pipette shaft ahead vertically to a pipette tip stored in a support position, wherein the pipette tip is attached to a coupling point on the pipette shaft.
  • the pipetting unit is moved by means of the positioning device to a liquid receiving position and there the pipette tip is immersed with its mouth opening in a liquid-filled storage vessel and here by the action of a displacing means in the pipetting unit, which is flow-connected to the pipette tip and a Under pressure and overpressure can produce, liquid added to the pipette tip.
  • the pipetting unit In the coupling position, the pipetting unit is coupled to the microdosing unit. e) The pipetting unit is moved together with the microdosing unit by means of the positioning device to one or more liquid dispensing positions, so that the mouth opening is arranged above a target vessel, and there the microdosing unit for dropwise liquid dispensing is taken into operation, preferably several liquid dispensing positions started and the liquid discharge repeated.
  • the pipetting unit is moved by means of the positioning device to a pipette tip ejection position, where the pipette tip is dropped into a receptacle by means of a tip ejector.
  • the pipette tip is positioned relative to an actuating device of the microdosing unit such that a lower section of the pipette tip immediately above the outlet opening always remains underneath the actuating device and does not touch it.
  • the displacing means may expel liquid from the pipette tip so that the meniscus of the liquid is formed at the mouth of the pipette tip.
  • the microdosing unit is put into operation between method steps d) and e), with its actuating device acting in a reciprocating motion on a tube located at the lower end of the pipette tip, which is arranged in front of the mouth opening, such that this is deformed radially elastic and volume reducing so that the liquid is discharged in the tube in the form of free-flying droplets or in the form of a free-flying jet.
  • the coupling and decoupling of the microdosing unit to the pipetting unit within the scope of the preferred method can preferably take place by means of switched coupling devices, wherein preferably a coupling device is a magnetic coupling, further preferably a coupling device with a currentable permanent magnet / electromagnet.
  • a residual liquid in the pipette tip can be passed between the process steps e) and f) through the process.
  • ejected unit which preferably takes place in a separate liquid dispensing position.
  • the displacer unit will feed this liquid volume as an overpressure directly or discontinuously. Due to its design, liquid of smallest volumes, volumes up to 0.1 nl, held by a pipetting unit pipette tip can be transferred from a storage vessel to one or more target vessels with the inventive method.
  • the pipette tip has a tube arranged in front of its mouth opening.
  • the coupling of the pipette tip to a pipetting unit of the pipetting device, the picking up of the liquid, possibly the dispensing of residual liquid and the ejection of the pipette tip take place as in conventional pipetting devices.
  • the dispensing of the liquid in very small volumes takes place by means of a microdosing unit, which is switchably connected to the pipetting unit after the liquid has been taken up and later decoupled again.
  • the pipetting unit is moved by means of the positioning device to a coupling position and there the pipette tip is moved with its tube between an abutment and an actuating device of the micro-dosing unit.
  • the abutment is attached to the tube.
  • the pipetting unit with its coupling device is also coupled to the counter-coupling device of the micro-dosing unit, and, preferably, in the coupling position via the pipetting unit, the micro-dosing unit is connected to the control device with control technology.
  • the pipetting unit is then moved with the microdosing unit by means of the positioning device to one or more liquid dispensing positions, so that the orifice of the corresponding pipette tip is arranged above a target vessel, and there the microdosing unit for droplet-like liquid dispensing is put into operation.
  • the pipetting unit is then moved back to the coupling position by means of the positioning device, where the coupling device is uncoupled from the counter coupling device, the abutment is moved away from the tube into an initial position and the microdosing unit is switched off again.
  • FIG. 2 is a schematic representation of the pipetting device in a pipette tip receiving position
  • FIG. 3 is a schematic representation of the pipetting device in a liquid receiving position
  • FIG. 6 is a schematic representation of the pipetting device in a pipette tip ejection position
  • FIG. 7 shows a pipetting device according to the invention with four parallel pipette tips and a quadruple microdosing unit in a schematic representation.
  • FIG. 1 shows a pipetting device with a pipetting unit 1. At least one pipette tip 2 can be replaceably attached to the pipetting unit 1.
  • the pipetting device is a multi-channel pipetting device. Then there are several pipette tips 2 at the pipetting unit 1.
  • the pipetting device according to the invention is one having a pipette tip 2, which has an elastically deformable ejection end 3.
  • the elastically deformable discharge end 3 has the shape of an elastically deformable tube in the illustrated embodiment.
  • Such a tube may have any cross section, for example, an elliptical cross section.
  • the pipetting device has a positioning device 4 and a microdosing unit 5.
  • the entire pipetting device is completed by an electrical, electronic, often software-controlled control device 6, by which all processes of the pipetting device can be controlled as desired.
  • Fig. 1 is indicated by dashed lines where transmission means for control commands, power supply and / or data are provided between the individual components of the pipetting device.
  • the pipette unit 1 has a coupling point 7 for each attachable pipette tip 2.
  • This is typically designed in the form of a pipette shaft.
  • the pipette tip 2 is plugged onto the coupling point 7 formed by the pipette shaft or the coupling point 7 is inserted into the open upper end of the pipette tip 2 in order to couple the pipette tip 2 stored in a holder or support frame 16.
  • the pipette unit 1 has a displacer unit 8 fluidically connected to the coupling point 7 and the pipette tip 2 attached thereto.
  • the displacer unit 8 is typically a cylinder-piston arrangement. It is essential that the displacer unit 8 in the coupled pipette tip 2 can generate a negative pressure for taking up liquid in the pipette tip 2 and an overpressure for ejecting liquid from the pipette tip 2. Also, reference may be made to the entire state of the art explained in the introduction. By means of the positioning device 4, the pipetting unit 1 can be brought into different operating positions.
  • Typical operating positions are a pipette tip receiving position ( Figure 2), a fluid receiving position ( Figure 3), a docking position ( Figure 4), a fluid dispensing position ( Figure 5) or a plurality of such fluid dispensing positions, and a Pipette tip ejection position (Fig. 6).
  • residual liquid ejection position In between can also be a residual liquid ejection position, which is not shown in the drawing.
  • residual liquid ejection position of the pipette unit 1 residual liquid from the pipette tips 2 may be discarded before being dropped into a container at the pipette tip ejection position.
  • the microdosing unit 5 shown in FIG. 4 is controlled by its own control unit 15.
  • the microdosing unit 5 In order to interact with the elastically deformable discharge end 3, the elastically deformable tube realized here directly above the mouth opening of the pipette tip 2, the microdosing unit 5 has a movable actuating device 9.
  • the microdosing unit 5 is arranged in the dispensing position of the pipette unit 1 relative to the pipette tip 2 such that the actuating device 9 is positioned on the elastically deformable ejection end 3 of the pipette tip 2.
  • the ejection end 3 of the pipette tip 2 can be deformed radially elastically in a volume-displacing manner by means of the actuating device 9 by driving the microdosing unit 5.
  • one or more liquid can be ejected from the discharge end 3 of the pipette tip 2 as the smallest droplets or as a free-flowing jet.
  • FIGS. 2 to 7 show the construction of the microdosing unit 5 with reference to the pipetting unit 1. It is provided that the microdosing unit 5 is executed separately from the pipetting unit 1 but can be coupled with the pipetting unit 1 in a precisely defined relative position such that the pipetting unit 1 has a coupling device 10 and the microdosing unit 5 has a corresponding negative feedback device 11 Coupling device 10 and / or the negative feedback device 1 1 is switchable and that by switching the coupling device 10 and / or the negative feedback device 1 1, the Mikrodosierillon 5 can be coupled either to the pipetting unit 1 or decoupled from the pipetting unit 1. The microdosing unit 5 therefore does not always have to be coupled to the pipetting unit 1.
  • the pipetting device has a storage platform 12 for storing the microdosing unit 5 when not in use.
  • the storage platform 12 provides the deliberately provided, defined space for storage of the microdosing unit 5 when not in use. It is expediently positioned in a fixed position throughout the pipetting device. Not shown in FIG. 4 is a positioning aid between the pipetting device or the deposition platform 12 on the one hand and the microdosing unit 5 on the other hand for exact and positionally stable positioning of the microdosing unit 5.
  • the coupling device 10 and the negative feedback device 11 there are in principle a variety of possibilities. In principle, it would be possible to mechanically implement the coupling device 10 and the counter coupling device 1 1, that is to say as a groove / tongue connection or as a bolt connection, etc. In principle, it would also be possible to provide only manual actuation. This is not normally done in the context of a largely automated petting device.
  • the negative feedback device 11 is active and switchable by the control device 6 or the control unit 15 and the coupling device 10 is made passive.
  • the configuration is recommended as a switched magnetic coupling.
  • a switched magnetic coupling can generate a coupling effect when a solenoid is energized.
  • the electromagnet would have during the entire process with the micro-dosing unit 5 at the Pipetting 1 are energized.
  • the associated power consumption would be comparatively high.
  • the coupling device 10 in the invention in the Martinezkopplungsein- device 11 (or the coupling device 10 for the aforementioned alternative case) is therefore constructively worked with at least one permanent magnet 13 and the permanent magnet 13 associated electromagnet 14.
  • the electromagnet 14 When the electromagnet 14 is not energized, the holding force of the permanent magnet 13 causes the microdosing unit 5 to be coupled to the pipette unit 6.
  • the coupling device 10 has a simple counterpart 10 'of ferromagnetic material.
  • the electromagnet 14 is energized with current in one direction, which leads to a magnetic field of the permanent magnet 13 opposite, but approximately the same size magnetic field, the magnetic force of the permanent magnet 13 is neutralized.
  • the electromagnet 14 is energized, a decoupling of the negative feedback device 1 1 from the coupling device 10 and thus the microdosing unit 5 is effected by the pipetting unit 1.
  • One can further optimize the control of the electromagnet 14 so that it can also be energized in the opposite direction.
  • the magnetic force of the energized electromagnet 14 amplifies that of the permanent magnet 13, so that the microdosing unit 5 is very particularly firmly coupled to the pipetting unit 1.
  • This selective energization of the electromagnet 14 can be realized, for example, when sections act on high accelerations on the microdosing 5 or otherwise the microdosing 5 is exposed to special forces.
  • Another advantage of the present design of the preferred embodiment is that when the magnetic force of the permanent magnet 13 is neutralized, the pipetting unit 1 can be positioned freely and easily relative to the microdosing unit 5 by means of the positioning device 4. Only when the defined relative position is reached, which can be achieved, for example, by means of corresponding positioning aids, are the permanent magnets 13 released in terms of their magnetic effect.
  • the illustrated and preferred embodiment shows a modern, combined element of permanent magnet 13 and electromagnet 14.
  • Such integrated elements are particularly compact and have a particularly high performance.
  • a different distribution of the components is possible. For example, you can run the negative feedback device with energizable electromagnet, the coupling device, however, with the permanent magnet, which interact with the opposing electromagnet. This alternative is not shown in the drawing.
  • the microdosing unit 5 is connected in terms of control technology by coupling to the pipetting unit 1 with the control device 6 of the pipetting device.
  • the microdosing unit 5 may be autonomous in terms of control technology, wherein it communicates with the control device 6 of the pipetting device in a contact-bound or wireless manner.
  • an electrical contact connection between the pipetting device and microdosing unit 5 is established.
  • control signals or the voltage supply or charging currents for rechargeable batteries or capacitors of the microdosing unit 5 can be conducted via this contact connection.
  • the electrical contact connection can also be arranged between the pipetting unit 1 and microdosing unit 5 and connected to the coupling.
  • the microdosing unit 5 has its own control unit 15, which has already been mentioned above. Suggestions for this will also be described later in the discussion of the embodiment. In the following, the mode of operation with a pipetting device according to the invention will be briefly shown again with reference to FIGS. 2 to 6.
  • Fig. 3 shows the situation in the pipette tip receiving position.
  • the pipette tip 2 is suspended in the support frame 16 of a storage container.
  • the displacer unit 8 in the form of a cylinder-piston arrangement is located together with the associated drive 17 on a holder 18.
  • the control device 6 of the pipetting device is indicated purely schematically on the drive 17. Of course, this can also be arranged at any other location. Arrows indicate how the pipetting unit 1 shown here can be moved by means of the positioning device 4.
  • the pipette tip 2 has been plugged onto the coupling point 7.
  • the pipetting unit 1 has been brought into the liquid receiving position by means of the positioning device 4.
  • the piston of the displacer unit 8 can be moved a little further and suck some air, so that no liquid drips from the mouth of the pipette tip 2, when the pipetting unit 1 to another operating position is moved.
  • FIG. 4 shows a representation of the coupling position.
  • the pipetting unit 1 has been brought together with the microdosing unit 5.
  • the coupling device 10 on the pipetting unit 1 interacts with the counter coupling device 11 in such a way that the microdosing unit 5 is firmly coupled to the pipetting unit 1.
  • the actuator 9 of the microdosing unit 5 shown in FIG. 4 naturally has to be located exactly where the discharge end 3 of the associated pipette tip 2 is located.
  • the arrow indicates that the clamping jaw 20 is set against the discharge end 3 of the pipette tip 2, so that there is an abutment for the movement of the actuating device 9 results.
  • the conditioning of the pipette unit 1 is also carried out in the coupling position according to FIG. 4.
  • the piston of the displacer unit 8 is moved downwards so far (arrow) until the meniscus of the liquid located therein is formed at the outlet opening of the ejection end 3.
  • a vessel 21 is arranged in Fig. 4, in which normally no liquid is yet. This should now be discharged into the vessel 21.
  • the microdosing unit 5 is put into operation.
  • With a reciprocating movement of the actuator 9 is formed on the designed as an elastically deformable tube ejection end 3 of the pipette tip radially Force applied, so that this is deformed.
  • liquid in droplet form is discharged from the mouth opening of the pipette tip 2 into the vessel 21.
  • the movement of the actuating device 9 is indicated in Fig. 5 with a double arrow. It takes place back and forth against the abutment forming jaw 20. This is first the conditioning of the pipetting in preparation for the actual liquid delivery.
  • Fig. 5 shows the actual liquid dispensing in the liquid dispensing position.
  • target vessels 21 ' for example the wells of a microtiter plate, and above the direction of movement of the pipetting unit 1 together with the microdosing unit 5, moved by the positioning device 4.
  • Shown is a deformation effect of the actuator 9 of the microdosing unit 5 from the discharge end 3 of the pipette tip 2 small liquid drop blasted, for example, 0.5 l volume.
  • the displacer unit 8 of the pipetting unit 1 can be controlled in accordance with the dispensing of liquid by means of the microdosing 5 so that the dispensed minimum volume of liquid after a number of liquid discharges discontinuously or directly into the discharge end 3 of the pipette tip 2 is fed into it.
  • the pressure in the air cushion in the pipette tip 2 is kept constant.
  • This sequence of the pipetting device according to the invention is also shown in Fig. 5. One can understand the relatively small displacement movement of the piston of the displacer unit 8 by means of the arrow.
  • the microdosing unit 5 is first turned off again at the coupling position on its depositing platform 12, the clamping jaw 20 is moved away from the ejecting end 3 into the starting position and the coupling device 10; 1 1 solved.
  • the storage platform 12 for example, indicated in the microdosing unit 5 batteries 22 are automatically recharged.
  • a contact-bound electrical connection for the charging current and / or for a data exchange with the control device 6 is located between the microdosing unit 5 and the storage platform 12.
  • Fig. 6 the end position of the pipetting unit 1 is then shown after a completed pipetting process.
  • the pipetting unit 1 is again by itself and has been brought as such by means of the positioning device 4 in the pipette tip dropping position.
  • the tip ejector 23 By means of the tip ejector 23, the pipette tip 2 is dropped into a receptacle. This collection container is not shown here.
  • the vertical arrows in Fig. 6 indicate the movement of the Spitzenabschitz worn 23 and due to the pipette tip 2 at.
  • FIG. 7 shows details of a pipetting device with a plurality of pipette tips 2, namely four pipette tips 2, and with a plurality of displacing units 8, namely a cylinder-piston arrangement per coupling point 7 with pipette tip 2.
  • the piston rods of the cylinder Piston arrangements which form the displacer unit 8 are brought together on a common drive 17. Below you can see the jaws 20 and the target vessels 21st

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Human Computer Interaction (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif de pipette comportant une unité de pipette (1), un dispositif de positionnement (4), une unité de microdosage (5) et un dispositif de commande (6). L'unité de microdosage (5) est séparée de l'unité de pipette (1), mais elle peut être couplée à l'unité de pipette (1) en une position relative précisément définie. À cet effet, l'unité de pipette (1) comporte un dispositif d'accouplement (10) et l'unité de microdosage (5) comporte un dispositif d'accouplement complémentaire (11), un de ces dispositifs étant commutable. La commutation du dispositif d'accouplement (10) et/ou du dispositif d'accouplement complémentaire (11) permet sélectivement d'accoupler l'unité de microdosage (5) à l'unité de pipette (1) ou de la désaccoupler de celle-ci (1). Lorsque l'unité de microdosage (5) est couplée à l'unité de pipette (1), elle peut être mise dans différentes positions de fonctionnement, conjointement avec l'unité de pipette (1).
EP14726510.2A 2013-04-11 2014-04-10 Dispositif de pipette comportant une unite de microdosage Active EP2983826B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202013003390.8U DE202013003390U1 (de) 2013-04-11 2013-04-11 Pipettiervorrichtung mit einer Mikrodosiereinheit
PCT/EP2014/000965 WO2014166635A1 (fr) 2013-04-11 2014-04-10 Dispositif de pipette comportant une unite de microdosage

Publications (2)

Publication Number Publication Date
EP2983826A1 true EP2983826A1 (fr) 2016-02-17
EP2983826B1 EP2983826B1 (fr) 2017-09-13

Family

ID=50828853

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14726510.2A Active EP2983826B1 (fr) 2013-04-11 2014-04-10 Dispositif de pipette comportant une unite de microdosage

Country Status (4)

Country Link
US (1) US10058859B2 (fr)
EP (1) EP2983826B1 (fr)
DE (1) DE202013003390U1 (fr)
WO (1) WO2014166635A1 (fr)

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DE102016008101A1 (de) 2016-07-05 2018-01-11 Brand Gmbh + Co Kg Pipettiervorrichtung zum Aspirieren und Dispensieren von Flüssigkeiten
DE202016004140U1 (de) 2016-07-05 2017-10-08 Brand Gmbh + Co Kg Pipettiervorrichtung zum Aspirieren und Dispensieren von Flüssigkeiten
US10625254B2 (en) * 2017-11-22 2020-04-21 Brand Gmbh + Co Kg Method for controlling a pipetting device
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US10058859B2 (en) 2018-08-28
US20160067705A1 (en) 2016-03-10
WO2014166635A1 (fr) 2014-10-16
EP2983826B1 (fr) 2017-09-13

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