EP1036594B1 - Générateur multicanaux de gouttelettes - Google Patents

Générateur multicanaux de gouttelettes Download PDF

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Publication number
EP1036594B1
EP1036594B1 EP00104462A EP00104462A EP1036594B1 EP 1036594 B1 EP1036594 B1 EP 1036594B1 EP 00104462 A EP00104462 A EP 00104462A EP 00104462 A EP00104462 A EP 00104462A EP 1036594 B1 EP1036594 B1 EP 1036594B1
Authority
EP
European Patent Office
Prior art keywords
outlets
distributor
liquid
cross
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP00104462A
Other languages
German (de)
English (en)
Other versions
EP1036594A2 (fr
EP1036594A3 (fr
Inventor
Thomas Moore
Sebastian Pobering
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cybio Instruments GmbH
Original Assignee
Cybio AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cybio AG filed Critical Cybio AG
Publication of EP1036594A2 publication Critical patent/EP1036594A2/fr
Publication of EP1036594A3 publication Critical patent/EP1036594A3/fr
Application granted granted Critical
Publication of EP1036594B1 publication Critical patent/EP1036594B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • Multi-channel drop generators or dispensing devices are used in particular for filling cavities of microtitration plates with liquid. They usually consist of a pump and a distributor connected to it via a line.
  • the solutions known from the prior art pursue the goal of filling cavities with the smallest possible differences in volume, that is to say that the same volume is delivered at all outlets of the distributor at any time.
  • different constructive solutions for the distributor have been developed.
  • the distributor is constructed as a tube distributor. However, this prohibits an exactly uniform distribution due to its functional principle. Outputs whose distance to the input of the distributor is shorter than more distant outputs are preferably flowed through.
  • This consists in principle of a horizontal distribution pipe, from which at least two filling pipes lead first upwards and then after a deflection area in an outflow area down.
  • the filling tubes are connected to one another via a compensating tube.
  • the line between the pump and the distributor tube should be made of elastic material to compensate for the relatively hard pump strokes and to withdraw the liquid in the filling tubes after completion of each pumping by about 1 - 2 mm, which dripping is to be prevented safely.
  • the invention has the object of providing a multichannel drop generator which is capable of transferring liquids even without static charging several outlets in the same amount in a volume range of about 0.5-10 ⁇ l leave.
  • the multi-channel drop generator is basically constructed and operated so that the delivery on the one hand contactless and on the other hand via outlets, which are crucial for Completely empty the accuracy of the volume delivery after each delivery become.
  • the metering device 1 consists of a storage vessel 5 and a metering device 6, the liquid from the storage vessel 5 via the connection 2 in a manifold 3 at its outputs outlets 4 are arranged, which allow a discharge of the droplets conveyed.
  • the amount of liquid must be precisely adjustable and promoted with high repeatability. Solenoid valves in conjunction with a pressure vessel or wobble piston pumps can be used advantageously.
  • the strong pulsation of the metering devices 1 generates a pressure pulse, which is transmitted via the connection 2 to the distributor 3 and to the outlets 4 and which allows the dispensing of small drops there.
  • the connection is a preferably hose-like component of defined elasticity in order to reduce the edge steepness of the pressure pulses.
  • the distributor 3 is a planar channel system of cascaded T-pieces having an input 12 and a plurality of outputs 13, as shown in Figs. 1 and 2, which may for example be incorporated in plate material. Such a structure ensures with minimum dead volume an exactly uniform distribution of the liquid flowing through the outputs, regardless of their fluidic properties. Furthermore, the distributor 3 causes a uniform distribution of the pressure pulses from the input to the outputs. Due to the large compared to the outlets 4 cross-section of the channels of the channel system, the distributor 3 has only a negligible proportion of the dynamic properties of the multi-channel drop generator.
  • Outlets 4 are arranged at the outlets, which represent a cross-sectional constriction and thus form the largest fluidic resistance in the multichannel drop generator.
  • the outlets 4 In order for the outlets 4 to form an equal fluidic resistance, they must have a high manufacturing accuracy, which are met for example in precision tubes. Manufacturing tolerances within the channel system are thereby compensated.
  • the liquid column within the outlets 4 has the greatest inertia in the overall system and therefore has a significant influence on its dynamic behavior.
  • a dynamic consideration is required, in particular for the delivery of very small droplets, via the observation of the static parameters, which is to take place on the basis of an electrical equivalent circuit diagram. From Fig.
  • the metering device 1 conveys the volume to be delivered at a high transport speed via the connection 2 and the distributor 3 to the outlets 4. There form liquid jets with the diameter of the outlets 4. The inertia prevents dripping. If the liquid flow is interrupted abruptly, the jet breaks off due to the high kinetic energy before a drop is formed. However, even here an indefinite amount of liquid remains at the outlets 4 and forms a meniscus. In order not to falsify the result of the following delivery cycle, this liquid residue must be removed. This could be done by an inverse operation of the metering device 1, which would, however, lead to a greatly increased technical complexity due to the high speeds.
  • connection 2 designed as an elastic element, which is capable of assuming this function.
  • the compound 2 As its cross-section expands, takes up part of the delivered volume and partially releases it again later. If the volume flow is interrupted, causes the inertia of the liquid columns in the outlets 4 for a short-term continuance of the flow. The flowing volume is supplied by the elasticity of the connection 2, whereby a negative pressure arises. If the liquid columns come to a standstill, this negative pressure causes a sucking back of the liquid residues from the outlets 4.
  • the chronological sequence of a dosing cycle (shown in FIG.
  • V D represents the volumetric flow generated by the dosing device 1 and V A represents the volumetric flow produced at the outlets
  • V A represents the volumetric flow produced at the outlets

Claims (1)

  1. Générateur de gouttes multi-canaux comportant un dispositif de dosage (1), un distributeur (3) comportant une entrée (12) et plusieurs sorties (13), ainsi qu'une connexion élastique (2) par laquelle le dispositif de dosage (1) est relié à l'entrée (12), caractérisé en ce que l'entrée (12) est reliée aux sorties (13) par un système de canaux planaire de pièces en T cascadantes, ce qui a pour effet une distribution homogène de pression,
    en ce qu'il y a aux sorties (13) des ouvertures d'évacuation (4) tubulaires dont la section transversale est plus faible que la section transversale des canaux dans le distributeur (3) et dont la longueur et la section transversale sont adaptées à l'élasticité, à la longueur et à la section transversale de la connexion élastique (2) et à la vitesse de transport du dispositif de dosage (1), de sorte que le générateur de gouttes multi-canaux présente un comportement dynamique, ce qui a pour effet une décharge de liquide sans contact et, puis, un vidage complète des ouvertures d'évacuation (4).
EP00104462A 1999-03-08 2000-03-07 Générateur multicanaux de gouttelettes Expired - Lifetime EP1036594B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19911456A DE19911456A1 (de) 1999-03-08 1999-03-08 Mehrkanal-Tropfengenerator
DE19911456 1999-03-08

Publications (3)

Publication Number Publication Date
EP1036594A2 EP1036594A2 (fr) 2000-09-20
EP1036594A3 EP1036594A3 (fr) 2001-01-24
EP1036594B1 true EP1036594B1 (fr) 2005-06-15

Family

ID=7901017

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00104462A Expired - Lifetime EP1036594B1 (fr) 1999-03-08 2000-03-07 Générateur multicanaux de gouttelettes

Country Status (3)

Country Link
EP (1) EP1036594B1 (fr)
JP (1) JP2000258438A (fr)
DE (2) DE19911456A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10000691A1 (de) * 2000-01-10 2001-07-26 Fraunhofer Ges Forschung Mikro-Düsen-System
DE10236029A1 (de) 2002-08-02 2004-02-19 Cybio Systems Gmbh Einrichtung zum Dispensieren und Beobachten der Lumineszenz von Einzelproben in Multiprobenanordnungen
DE10255595A1 (de) 2002-11-26 2004-06-03 Cybio Ag Mehrkanaldosiervorrichtung mit automatischer Kalibrierung
ITMI20031900A1 (it) * 2003-10-03 2005-04-04 Delta Biolog S R L Dispositivo per la distribuzione e l'aspirazione uniforme di piccole quantita' di fluidi.
DE102006030068A1 (de) 2006-06-28 2008-01-03 M2P-Labs Gmbh Vorrichtung und Verfahren zur Zu- und Abfuhr von Fluiden in geschüttelten Mikroreaktoren Arrays
CN104880353B (zh) * 2015-06-11 2017-12-29 中国矿业大学 一种多通道气流均匀润湿煤样装置
DE102016015700A1 (de) 2016-12-21 2018-06-21 Bayer Pharma Aktiengesellschaft Dosiervorrichtung
CN114225987B (zh) * 2021-11-15 2023-03-07 北京理工大学 一种可分别控制的双液滴生成装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL24894A (en) * 1965-12-29 1969-08-27 Yeda Res & Dev Container for dispensing drop-size quantities of liquids
US4314259A (en) * 1980-06-16 1982-02-02 Arthur D. Little, Inc. Apparatus for providing an array of fine liquid droplets particularly suited for ink-jet printing
AT379455B (de) * 1984-04-10 1986-01-10 Prolic Ag Dispensiereinrichtung fuer fluessigkeiten
US4730197A (en) * 1985-11-06 1988-03-08 Pitney Bowes Inc. Impulse ink jet system
US5334352A (en) * 1992-09-23 1994-08-02 Icn Biomedicals, Inc. Manifold construction
US5441204A (en) * 1993-06-10 1995-08-15 United Air Specialists, Inc. Electrostatic fluid distribution nozzle

Also Published As

Publication number Publication date
DE50010538D1 (de) 2005-07-21
EP1036594A2 (fr) 2000-09-20
DE19911456A1 (de) 2000-09-14
EP1036594A3 (fr) 2001-01-24
JP2000258438A (ja) 2000-09-22

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