WO2011107077A3 - Fusionspeptide zur verstärkung der expression rekombinanter polypeptide - Google Patents

Fusionspeptide zur verstärkung der expression rekombinanter polypeptide Download PDF

Info

Publication number
WO2011107077A3
WO2011107077A3 PCT/DE2011/000206 DE2011000206W WO2011107077A3 WO 2011107077 A3 WO2011107077 A3 WO 2011107077A3 DE 2011000206 W DE2011000206 W DE 2011000206W WO 2011107077 A3 WO2011107077 A3 WO 2011107077A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
expression
yfp
cfp
coding sequence
Prior art date
Application number
PCT/DE2011/000206
Other languages
English (en)
French (fr)
Other versions
WO2011107077A9 (de
WO2011107077A2 (de
Inventor
Michael Berger
Georgi Muskehlishvili
Original Assignee
Jacobs University Bremen Ggmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jacobs University Bremen Ggmbh filed Critical Jacobs University Bremen Ggmbh
Priority to DE112011100728T priority Critical patent/DE112011100728A5/de
Publication of WO2011107077A2 publication Critical patent/WO2011107077A2/de
Publication of WO2011107077A9 publication Critical patent/WO2011107077A9/de
Publication of WO2011107077A3 publication Critical patent/WO2011107077A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Nach dem bisherigen Stand der Technik sind CFP und YFP in E.coli nicht exprimierbar. Dadurch das drei N-terminale Peptide codierende Sequenzen auf ihren Effekt auf die Expression von Cyan fluoresierenden Protein (CFP) und gelbfluoresierenden Protein (YFP) in Escherichia Coli untersucht wurden. Die translationale Fusion der codierenden Sequenz für das N-terminale Peptid von RpIL führt dabei zur stärksten Expression von CFP und YFP und sollte daher im Allgemeinen zur Verstärkung der Überexpression heterologer Proteine für wissenschaftliche und kommerzielle vorgeschlagen. Dadurch betrifft das erfinderische Verfahren ein Verfahren zum Herstellen einer ersten codierenden Sequenz für ein Peptid und/oder ein Protein zur Translationsverstärkung, wobei die erste codierende Sequenz ein N-terminales Peptid und/oder Protein zur Translationsverstärkung codiert und das N-terminale Peptid und/oder Protein insbesondere zwischen zwei und 30 Aminosäuren und ein erstes Zielprotein aufweist, wobei das Verfahren den Schritt des Fusionieren von einem Leserahmen der ersten codierenden Sequenz mit einem Leserahmen des ersten Zielproteins mittels molekulartechnischer Methoden umfasst.
PCT/DE2011/000206 2010-03-02 2011-03-02 Fusionspeptide zur verstärkung der expression rekombinanter polypeptide WO2011107077A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112011100728T DE112011100728A5 (de) 2010-03-02 2011-03-02 Verfahren zum herstellen einer codierenden sequenz für ein peptid, protein zur translationsverstärkung, codierende sequenz, expressionsvektoren

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102010009996.1 2010-03-02
DE201010009996 DE102010009996A1 (de) 2010-03-02 2010-03-02 Verfahren zum Herstellen einer ersten codierenden Sequenz für Peptid, Protein zur Translationsverstärkung, codierende Sequenz, Expressionsvektor

Publications (3)

Publication Number Publication Date
WO2011107077A2 WO2011107077A2 (de) 2011-09-09
WO2011107077A9 WO2011107077A9 (de) 2011-12-29
WO2011107077A3 true WO2011107077A3 (de) 2012-03-08

Family

ID=44502818

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2011/000206 WO2011107077A2 (de) 2010-03-02 2011-03-02 Fusionspeptide zur verstärkung der expression rekombinanter polypeptide

Country Status (2)

Country Link
DE (2) DE102010009996A1 (de)
WO (1) WO2011107077A2 (de)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003255339A1 (en) * 2002-07-31 2004-02-23 Centre National De La Recherche Scientifique Fusion proteins between a fluorescent protein and an ionotropic receptor and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KATZKE N ET AL: "A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 69, no. 2, 1 February 2010 (2010-02-01), pages 137 - 146, XP026756187, ISSN: 1046-5928, [retrieved on 20090823], DOI: 10.1016/J.PEP.2009.08.008 *
PETERSEN C: "Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12", JOURNAL OF BACTERIOLOGY 1990 US, vol. 172, no. 1, 1990, pages 431 - 436, XP002660389, ISSN: 0021-9193 *
VEENING JAN-WILLEM ET AL: "Visualization of differential gene expression by improved cyan fluorescent protein and yellow fluorescent protein production in Bacillus subtilis", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 70, no. 11, November 2004 (2004-11-01), pages 6809 - 6815, XP002660387, ISSN: 0099-2240 *
WAUGH ET AL: "Making the most of affinity tags", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 23, no. 6, 1 June 2005 (2005-06-01), pages 316 - 320, XP025290695, ISSN: 0167-7799, [retrieved on 20050601], DOI: 10.1016/J.TIBTECH.2005.03.012 *
ZIEGELHOFFER THOMAS ET AL: "Expression of Acidothermus cellulolyticus E1 endo-beta-1,4-glucanase catalytic domain in transplastomic tobacco.", PLANT BIOTECHNOLOGY JOURNAL AUG 2009 LNKD- PUBMED:19500296, vol. 7, no. 6, August 2009 (2009-08-01), pages 527 - 536, XP002660388, ISSN: 1467-7652 *

Also Published As

Publication number Publication date
WO2011107077A9 (de) 2011-12-29
WO2011107077A2 (de) 2011-09-09
DE112011100728A5 (de) 2013-01-03
DE102010009996A1 (de) 2011-09-08

Similar Documents

Publication Publication Date Title
AR114339A1 (es) Plataforma de presentación, productos y métodos con endosporas basadas en paenibacillus
WO2020102741A8 (en) Methods and compositions for protein sequencing
EA200870176A1 (ru) НОВЫЙ ПОВЕРХНОСТНЫЙ БЕЛОК HAEMOPHILUS INFLUENZAE (БЕЛОК E; pE)
WO2007106476A3 (en) Compositions and methods for enhancing the immunogenicity of antigens
NZ597685A (en) Proteins and nucleic acids from meningitis/sepsis-associated escherichia coli
ATE454393T1 (de) Egvii-endoglucanase und dafür codierende nukleinsäuren
WO2005033316A3 (de) Sekretion von proteinen aus hefen
WO2005086794A3 (en) Lactobacillus acidophilus nucleic acid sequences encoding stress-related proteins and uses therefor
WO2010011313A3 (en) Ligation of stapled polypeptides
CA2636075A1 (en) Chimaeric fusion protein with superior chaperone and folding activities
DK1798240T3 (da) Rekombinante triplex scaffold-baserede polypeptider
MX343386B (es) Proteinas de fusion portadoras de peptido como vacunas para alergia.
MX2021014057A (es) Polipéptidos de sintasas de cannabinoides optimizados.
MX2021000263A (es) Proteina de fusion variante sirpalfa-4-1bbl y procedimientos de uso de la misma.
WO2003048185A3 (en) Angiopioetin related factors
GB201102700D0 (en) Protein secretion
WO2011107077A3 (de) Fusionspeptide zur verstärkung der expression rekombinanter polypeptide
EP1651750A4 (de) Für fructooligosaccharid-nutzende verbindungen codierende nukleinsäuren aus lactobacillus acidophilus und verwendungen davon
MX2022003203A (es) Polipéptidos de sintasa de ácido tetrahidrocannabinólico (thca) optimizados.
DK1913020T3 (da) Immunogene konstrukter
MX2023000373A (es) Proteinas de fusion de proteinas de la espicula de coronavirus estabilizadas.
HUP0500349A2 (hu) Eljárás polipeptidek előállítására
Deng et al. Insulin chains as efficient fusion tags for prokaryotic expression of short peptides
US11492380B2 (en) PepCon proteomics standards and methods of use
WO2009125343A3 (en) Method for producing selenoproteins

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11730878

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 1120111007280

Country of ref document: DE

Ref document number: 112011100728

Country of ref document: DE

REG Reference to national code

Ref country code: DE

Ref legal event code: R225

Ref document number: 112011100728

Country of ref document: DE

Effective date: 20130103

122 Ep: pct application non-entry in european phase

Ref document number: 11730878

Country of ref document: EP

Kind code of ref document: A2