WO2005033316A3 - Sekretion von proteinen aus hefen - Google Patents

Sekretion von proteinen aus hefen Download PDF

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Publication number
WO2005033316A3
WO2005033316A3 PCT/EP2004/010346 EP2004010346W WO2005033316A3 WO 2005033316 A3 WO2005033316 A3 WO 2005033316A3 EP 2004010346 W EP2004010346 W EP 2004010346W WO 2005033316 A3 WO2005033316 A3 WO 2005033316A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
shuttle
peptides
acid sequences
proteins
Prior art date
Application number
PCT/EP2004/010346
Other languages
English (en)
French (fr)
Other versions
WO2005033316A2 (de
Inventor
Kai Ostermann
Gerhard Roedel
Original Assignee
Basf Ag
Kai Ostermann
Gerhard Roedel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Kai Ostermann, Gerhard Roedel filed Critical Basf Ag
Priority to JP2006525798A priority Critical patent/JP2007505602A/ja
Priority to US10/572,189 priority patent/US20070077619A1/en
Priority to CA002537492A priority patent/CA2537492A1/en
Priority to EP04765253A priority patent/EP1664306A2/de
Publication of WO2005033316A2 publication Critical patent/WO2005033316A2/de
Publication of WO2005033316A3 publication Critical patent/WO2005033316A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment And Processing Of Natural Fur Or Leather (AREA)
  • Surface Treatment Of Glass (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)

Abstract

Die vorliegende Erfindung betrifft Expressionskonstrukte, umfassend die kodierende Nukleinsäuresequenz für ein von Hefezellen prozessierbares Shuttlepeptidkonstrukt; entsprechende Expressionsvektoren, enthaltend derartige Konstrukte; mit deren Hilfe durchgeführte Verfahren zur rekombinanten Herstellung von Zielproteinen; damit transformierte Wirte; Shuttlepeptide und dafür kodierende Nukleinsäuresequenzen; Nukleinsäuresequenzen kodierend für solche Shuttlepeptide, fusioniert mit einem Fremdprotein; Hydrophobin-Proteine, welche unter Verwendung von derartigen Shuttlepeptiden hergestellt wurden, sowie die Verwendung Hydrophobinen zur Beschichtung von Gegenständen, wie z.B. Leder.
PCT/EP2004/010346 2003-09-15 2004-09-15 Sekretion von proteinen aus hefen WO2005033316A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006525798A JP2007505602A (ja) 2003-09-16 2004-09-15 酵母からのタンパク質の分泌
US10/572,189 US20070077619A1 (en) 2003-09-15 2004-09-15 Secretion of proteins from yeasts
CA002537492A CA2537492A1 (en) 2003-09-16 2004-09-15 Secretion of proteins from yeasts
EP04765253A EP1664306A2 (de) 2003-09-16 2004-09-15 Sekretion von proteinen aus hefen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10342794.5 2003-09-15
DE10342794A DE10342794A1 (de) 2003-09-16 2003-09-16 Sekretion von Proteinen aus Hefen

Publications (2)

Publication Number Publication Date
WO2005033316A2 WO2005033316A2 (de) 2005-04-14
WO2005033316A3 true WO2005033316A3 (de) 2005-10-06

Family

ID=34352870

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/010346 WO2005033316A2 (de) 2003-09-15 2004-09-15 Sekretion von proteinen aus hefen

Country Status (7)

Country Link
US (1) US20070077619A1 (de)
EP (1) EP1664306A2 (de)
JP (1) JP2007505602A (de)
CN (1) CN1852983A (de)
CA (1) CA2537492A1 (de)
DE (1) DE10342794A1 (de)
WO (1) WO2005033316A2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7892788B2 (en) 2005-02-07 2011-02-22 Basf Se Hydrophobin fusion products, production and use thereof
US20080319168A1 (en) * 2005-02-07 2008-12-25 Basf Aktiengesellschaft Method for Coating Surfaces with Hydrophobins
ATE483009T1 (de) * 2005-03-30 2010-10-15 Basf Se Verwendung von hydrophobinen zur schmutzabweisenden behandlung von harten oberflächen
MX2007012018A (es) * 2005-03-30 2007-11-16 Basf Ag Uso de hidrofobinas para el tratamiento de superficie de materiales de construccion de mineral endurecido, piedra natural, piedra artificial y ceramica.
EP1866150B1 (de) 2005-03-31 2016-10-19 Basf Se Metallische substrate mit polypeptiden als haftvermittler
US8535535B2 (en) 2005-04-01 2013-09-17 Basf Se Use of hydrophobin as a phase stabilizer
DK1869138T3 (da) 2005-04-01 2010-03-01 Basf Se Borevæske indeholdende hydrophobin
KR20080014034A (ko) * 2005-06-06 2008-02-13 바스프 악티엔게젤샤프트 섬유 기재 표면 코팅 방법
DE102005027039A1 (de) * 2005-06-10 2006-12-21 Basf Ag Hydrophobin als Beschichtungsmittel für expandierbare oder expandierte, thermoplastische Polymerpartikel
DE102005027139A1 (de) * 2005-06-10 2006-12-28 Basf Ag Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung
DE102005029704A1 (de) * 2005-06-24 2007-01-11 Basf Ag Verwendung von Hydrophobin-Polypeptiden sowie Konjugaten aus Hydrophobin-Polypeptiden mit Wirk-oder Effektstoffen und ihre Herstellung sowie deren Einsatz in der Kosmetik
EP1913143A1 (de) * 2005-07-29 2008-04-23 Pharmexa A/S Verbesserte proteinexpression
MX2008001056A (es) * 2005-08-01 2008-03-19 Basf Ag Uso de proteinas no enzimaticas de superficie activa para lavar textiles.
DE102005048720A1 (de) * 2005-10-12 2007-04-19 Basf Ag Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen
US8096484B2 (en) 2006-08-15 2012-01-17 Basf Se Method for the production of dry free-flowing hydrophobin preparations
CA2679719C (en) * 2007-03-12 2015-04-28 Basf Se Method of treating cellulosic materials with hydrophobins
US20110126143A1 (en) * 2009-11-24 2011-05-26 Williams Michael D System and method for interfacing fitness equipment and physiological monitoring
WO2012004255A1 (de) * 2010-07-07 2012-01-12 Basf Se Zusammensetzung enthaltend ein hydrophobin und verfahren zum reinigen von hydrophoben oberflächen
EP2631296A1 (de) 2012-02-22 2013-08-28 Kimmo Koivu Verfahren zur Hydrophobinherstellung in Pflanzen und Verfahren zur Herstellung von Hydrophobinmultimeren in Pflanzen und Mikroben
US20220143049A1 (en) 2019-03-21 2022-05-12 Onxeo A dbait molecule in combination with kinase inhibitor for the treatment of cancer
EP4054579A1 (de) 2019-11-08 2022-09-14 Institut National de la Santé et de la Recherche Médicale (INSERM) Verfahren zur behandlung von krebsarten, die resistenz gegen kinase-inhibitoren erworben haben
US20230234995A1 (en) * 2019-12-02 2023-07-27 Lg Chem, Ltd Cho cell-derived protein secretory factors and expression vectors comprising the same
WO2021148581A1 (en) 2020-01-22 2021-07-29 Onxeo Novel dbait molecule and its use
KR102466926B1 (ko) * 2020-05-20 2022-11-14 전남대학교산학협력단 하이드로포빈의 가용성 발현 방법 및 정제 방법

Citations (9)

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Publication number Priority date Publication date Assignee Title
EP0252561A2 (de) * 1986-07-11 1988-01-13 SCLAVO S.p.A. Expressions- und Sekretionsvektor in Hefe, verwendbar zur Herstellung von heterologen Proteinen
JPH06327481A (ja) * 1993-05-19 1994-11-29 Asahi Glass Co Ltd 分裂酵母接合フェロモン前駆体遺伝子
EP0662515A1 (de) * 1993-12-10 1995-07-12 Korea Institute Of Science And Technology Signalsequenzen für die Secretion heterologer Proteine von Hefe
WO1996041882A1 (en) * 1995-06-12 1996-12-27 Proefstation Voor De Champignoncultuur Hydrophobins from edible fungi, genes, nucleotide sequences and dna-fragments encoding for said hydrophobins, and expression thereof
EP0773296A1 (de) * 1995-02-03 1997-05-14 Asahi Glass Company Ltd. Gen für ein sekretionssignal und dieses enthaltender vektor
WO2002020651A2 (en) * 2000-09-06 2002-03-14 Zymogenetics, Inc. Human phermone polypeptide
WO2002046369A2 (en) * 2000-12-08 2002-06-13 Septegen Limited Yeast-based assays involving gpcrs
EP1223219A2 (de) * 1997-10-31 2002-07-17 Asahi Glass Company Ltd. Induzierbarer Promoter und Sekretionssignal zur Verwendung in Schizosaccharomyces pombe, Expressionsvektor diese enthaltend und ihre Verwendungen
WO2003053383A2 (fr) * 2001-12-14 2003-07-03 L'oreal Utilisation cosmetique d’au moins une hydrophobine pour le traitement des matieres keratiniques

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
GB2281421B (en) * 1993-08-23 1998-04-01 Advanced Risc Mach Ltd Integrated circuit
US6017731A (en) * 1996-12-13 2000-01-25 Chiron Corporation Method for expression of heterologous proteins in yeast

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0252561A2 (de) * 1986-07-11 1988-01-13 SCLAVO S.p.A. Expressions- und Sekretionsvektor in Hefe, verwendbar zur Herstellung von heterologen Proteinen
JPH06327481A (ja) * 1993-05-19 1994-11-29 Asahi Glass Co Ltd 分裂酵母接合フェロモン前駆体遺伝子
EP0662515A1 (de) * 1993-12-10 1995-07-12 Korea Institute Of Science And Technology Signalsequenzen für die Secretion heterologer Proteine von Hefe
EP0773296A1 (de) * 1995-02-03 1997-05-14 Asahi Glass Company Ltd. Gen für ein sekretionssignal und dieses enthaltender vektor
WO1996041882A1 (en) * 1995-06-12 1996-12-27 Proefstation Voor De Champignoncultuur Hydrophobins from edible fungi, genes, nucleotide sequences and dna-fragments encoding for said hydrophobins, and expression thereof
EP1223219A2 (de) * 1997-10-31 2002-07-17 Asahi Glass Company Ltd. Induzierbarer Promoter und Sekretionssignal zur Verwendung in Schizosaccharomyces pombe, Expressionsvektor diese enthaltend und ihre Verwendungen
WO2002020651A2 (en) * 2000-09-06 2002-03-14 Zymogenetics, Inc. Human phermone polypeptide
US20030049726A1 (en) * 2000-09-06 2003-03-13 Holloway James L. Human phermone polypeptide
WO2002046369A2 (en) * 2000-12-08 2002-06-13 Septegen Limited Yeast-based assays involving gpcrs
WO2003053383A2 (fr) * 2001-12-14 2003-07-03 L'oreal Utilisation cosmetique d’au moins une hydrophobine pour le traitement des matieres keratiniques

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199507, Derwent World Patents Index; Class B04, AN 1995-047902, XP002319523 *
IMAI Y ET AL: "THE FISSION YEAST MATING PHEROMONE P-FACTOR: ITS MOLECULAR STRUCTURE, GENE STRUCTURE, AND ABILITY TO INDUCE GENE EXPRESSION AND G1 ARREST IN THE MATING PARTNER", GENES AND DEVELOPMENT, COLD SPRING HARBOR, NY, US, vol. 8, no. 3, 1994, pages 328 - 338, XP001120726, ISSN: 0890-9369 *
NAKARI-SETALA TIINA ET AL: "Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall: Effect on cell surface properties and immobilization", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 7, July 2002 (2002-07-01), pages 3385 - 3391, XP002319522, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
JP2007505602A (ja) 2007-03-15
CA2537492A1 (en) 2005-04-14
WO2005033316A2 (de) 2005-04-14
DE10342794A1 (de) 2005-04-21
EP1664306A2 (de) 2006-06-07
CN1852983A (zh) 2006-10-25
US20070077619A1 (en) 2007-04-05

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