WO2011099730A3 - 세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법 - Google Patents

세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법 Download PDF

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WO2011099730A3
WO2011099730A3 PCT/KR2011/000790 KR2011000790W WO2011099730A3 WO 2011099730 A3 WO2011099730 A3 WO 2011099730A3 KR 2011000790 W KR2011000790 W KR 2011000790W WO 2011099730 A3 WO2011099730 A3 WO 2011099730A3
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modifier
cell
physiological functions
identifying modulators
reference substance
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PCT/KR2011/000790
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French (fr)
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WO2011099730A2 (ko
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김대중
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메디스커브 주식회사
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Priority to JP2012551925A priority Critical patent/JP2013518581A/ja
Priority to CN2011800091607A priority patent/CN102782494A/zh
Priority to EP11742422.6A priority patent/EP2535709A4/en
Publication of WO2011099730A2 publication Critical patent/WO2011099730A2/ko
Publication of WO2011099730A3 publication Critical patent/WO2011099730A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/60—Analysis of geometric attributes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
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  • Computer Vision & Pattern Recognition (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

본 발명은 세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법을 제공한다. 본 발명은 자력선 방향으로 자화되는 자성물질의 패턴과, 세포내 물질과 결합한 표지물질의 패턴을 이미지화하여 세포 내에서 참조물질과 조절물질 간의 경쟁반응여부를 결정함으로써 세포내 물질의 생리활성 기능을 조절할 수 있는 조절물질을 검출할 수 있다. 본 발명은 약물과 같은 조절물질 및 참조물질에 대한 세포내 물질의 반응이 살아 있는 세포 안에서 일어나므로 조절물질과 참조물질 간의 경쟁반응이 실제 세포내 물질대사를 반영하는 장점이 있다. 본 발명은 소수의 참조물질을 이용하여 다수의 조절물질을 스크린닝할 수 있다는 장점을 제공하고, 참조물질의 도입에 의해 조절물질의 변형 작업 없이 조절물질의 세포내 작용, 즉 약효 등을 탐색할 수 있는 장점을 제공한다.
PCT/KR2011/000790 2010-02-12 2011-02-08 세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법 WO2011099730A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2012551925A JP2013518581A (ja) 2010-02-12 2011-02-08 細胞内で細胞内物質の生理活性機能を調節する調節物質を検出する方法
CN2011800091607A CN102782494A (zh) 2010-02-12 2011-02-08 用于侦测调节细胞里细胞成分活性的调节子的方法
EP11742422.6A EP2535709A4 (en) 2010-02-12 2011-02-08 METHOD FOR IDENTIFYING MODULATORS FOR MODULATING PHYSIOLOGICAL FUNCTIONS OF INTRA-CELLULAR COMPONENTS IN ONE CELL

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100013372A KR101028875B1 (ko) 2010-02-12 2010-02-12 세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법
KR10-2010-0013372 2010-02-12

Publications (2)

Publication Number Publication Date
WO2011099730A2 WO2011099730A2 (ko) 2011-08-18
WO2011099730A3 true WO2011099730A3 (ko) 2012-01-12

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PCT/KR2011/000790 WO2011099730A2 (ko) 2010-02-12 2011-02-08 세포 내에서 세포내 물질의 생리활성 기능을 조절하는 조절물질을 검출하는 방법

Country Status (6)

Country Link
US (1) US20110201016A1 (ko)
EP (1) EP2535709A4 (ko)
JP (1) JP2013518581A (ko)
KR (1) KR101028875B1 (ko)
CN (1) CN102782494A (ko)
WO (1) WO2011099730A2 (ko)

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
WO2014122275A1 (en) * 2013-02-08 2014-08-14 Fundación Pública Andaluza Progreso Y Salud Method for the diagnosis and monitoring of autoinmune diseases
WO2016013541A1 (ja) * 2014-07-23 2016-01-28 コニカミノルタ株式会社 分子標的薬を含有する標識剤

Citations (4)

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JPH0767693A (ja) * 1992-12-31 1995-03-14 Vicam Lp 生物体の検出方法
JP2006325600A (ja) * 2003-06-30 2006-12-07 Eisai R & D Management Co Ltd 磁性細胞およびその使用方法
KR20070119785A (ko) * 2006-06-16 2007-12-21 연세대학교 산학협력단 미세유체채널 및 자장을 이용한 아폽토틱 세포의 검침·분리방법 및 이를 위한 장치
KR100823684B1 (ko) * 2006-12-06 2008-04-21 한국전자통신연구원 바코드 ｄｎａ를 이용한 생물학적 표적 물질의 검출 방법

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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0767693A (ja) * 1992-12-31 1995-03-14 Vicam Lp 生物体の検出方法
JP2006325600A (ja) * 2003-06-30 2006-12-07 Eisai R & D Management Co Ltd 磁性細胞およびその使用方法
KR20070119785A (ko) * 2006-06-16 2007-12-21 연세대학교 산학협력단 미세유체채널 및 자장을 이용한 아폽토틱 세포의 검침·분리방법 및 이를 위한 장치
KR100823684B1 (ko) * 2006-12-06 2008-04-21 한국전자통신연구원 바코드 ｄｎａ를 이용한 생물학적 표적 물질의 검출 방법

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Title
See also references of EP2535709A4 *

Also Published As

Publication number Publication date
EP2535709A4 (en) 2013-07-10
JP2013518581A (ja) 2013-05-23
US20110201016A1 (en) 2011-08-18
CN102782494A (zh) 2012-11-14
EP2535709A2 (en) 2012-12-19
WO2011099730A2 (ko) 2011-08-18
KR101028875B1 (ko) 2011-04-12

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