WO2011096609A1 - Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine - Google Patents

Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine Download PDF

Info

Publication number
WO2011096609A1
WO2011096609A1 PCT/KR2010/000767 KR2010000767W WO2011096609A1 WO 2011096609 A1 WO2011096609 A1 WO 2011096609A1 KR 2010000767 W KR2010000767 W KR 2010000767W WO 2011096609 A1 WO2011096609 A1 WO 2011096609A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
gene
seq
athg1
aging
Prior art date
Application number
PCT/KR2010/000767
Other languages
English (en)
Korean (ko)
Inventor
이동희
김국진
이인철
김동수
Original Assignee
제노마인(주)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 제노마인(주) filed Critical 제노마인(주)
Priority to PCT/KR2010/000767 priority Critical patent/WO2011096609A1/fr
Publication of WO2011096609A1 publication Critical patent/WO2011096609A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266—Abscission; Dehiscence; Senescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Definitions

  • the present invention relates to ATHG1 protein, its genes, and uses thereof having the functions of regulating aging and stress resistance of plants.
  • Plant aging is the final stage of plant development, an age-dependent process of decay at the cellular, tissue, organ, or organismal level, leading to the lethal stage through the growth and development stages. As aging progresses, the plant gradually loses its ability to synthesize, and the cellular structure and macromolecules are sequentially degraded, resulting in loss of homeostasis of cells and eventually death (Thomas et al., 1993). The aging of these plants is genetically planned as a series of consecutive biochemical and physiological phenomena that are very sophisticated and active at the level of cells, tissues and organs.
  • the initial phenomenon of aging in cell structure is the degradation of chloroplasts, which are organelles containing more than 70% of leaf protein. In metabolic terms, this means that carbon assimilation in plants is converted to catabolism of chlorophyll and macromolecules such as proteins, membrane lipids, and RNA. Increased catabolic activity through aging induces the conversion of cellular components accumulated in the assimilated leaves during growth into ventilated cellular components supplied for the development of seeds or other storage organs.
  • aging of plants is thought to be a process of cell degeneration as well as a genotype actively acquired to adapt to the environment during evolution (Buchanan-Wollaston et al., 2003; Lim and Nam, 2005; Nam, 1997).
  • cytokinin is a physiologically delayed aging hormone and many aging control techniques have been reported.
  • the Amasino group developed a method for regulating aging-specific cytokinin synthesis by recombining the IPT gene into a senescence-specific SAG12 gene promoter, which showed a 50% increase in productivity in cigarettes that delayed aging.
  • GmSARK in soybean one of the receptor-like kinases, is up-regulated not only in naturally occurring aging but also in the artificial aging process by cancer treatment. Inhibition of this gene is known to cause delay in leaf aging (Li et al. al., 2006).
  • ROS reactive oxygen species
  • An object of the present invention is to provide an ATHG1 protein having a aging retardation function and a stress resistance function of a plant.
  • Another object of the present invention is to provide a gene encoding the protein.
  • Still another object of the present invention is to provide a method for producing a plant having aging delay characteristics.
  • Another object of the present invention to provide a method for producing a stress resistant plant.
  • Still another object of the present invention is to provide a method for producing a plant having a yield increasing characteristic.
  • the present invention relates to an ATHG1 protein having a delaying function of aging of plants and a stress resistance function.
  • the inventor (s) is to isolate the gene of the protein based on the nucleotide sequence of the DNA binding protein-related protein (DNA-binding protein-related, GeneBank accession number NP 193515) as confirmed in the following Examples When the gene was transformed into the Arabidopsis overexpressed, delayed aging of the plant was clearly observed, and the characteristics of the increase in the size of the individual and the increase in seed production were also marked. It was found to exhibit resistance to oxidative stress and drought stress.
  • the ATHG1 protein of the invention is one of the polypeptides of (a), (b) and (c) below.
  • protein are used interchangeably with each other in the same sense as the polypeptide
  • gene is used interchangeably with each other in the same sense as the polynucleotide
  • a "polypeptide comprising a substantial portion of the amino acid sequence set forth in SEQ ID NO: 2" is still sufficient to retain plant aging delay and stress resistance functions as compared to the polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: And a polypeptide comprising a portion of the amino acid sequence of SEQ ID 2 of.
  • the length of the polypeptide and the degree of activity of such a polypeptide is not a problem since it still needs to be sufficient to retain the aging delaying and stress resistance functions of the plant.
  • polypeptide As such a polypeptide, the polypeptide which deleted the N-terminal part or C-terminal part in the polypeptide containing the amino acid sequence of SEQ ID NO: 2 is mentioned. This is because it is generally known in the art that even if the N- or C-terminal portion is deleted, such a polypeptide has the function of the original polypeptide. Of course, in some cases, the N-terminal or C-terminal moiety is necessary for maintaining the function of the protein, so that a polypeptide deleted with the N-terminal or C-terminal moiety does not exhibit this function. It is within the ordinary skill of one of ordinary skill in the art to distinguish and detect inactive polypeptides from active polypeptides.
  • the deletion of the N-terminal or C-terminal moiety as well as other moieties may still have the function of the original polypeptide.
  • one of ordinary skill in the art will be able to ascertain whether such deleted polypeptide still has the function of the original polypeptide within the scope of its usual ability.
  • the present specification discloses the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2, furthermore, a polypeptide encoded by the nucleotide sequence of SEQ ID NO: 1 and consisting of the amino acid sequence of SEQ ID NO: 2 delays aging function and stress resistance of plants.
  • the present invention confirms whether a polypeptide having a partial deletion in the amino acid sequence of SEQ ID NO: 2 still retains the function of the polypeptide including the amino acid sequence of SEQ ID NO: 2. It becomes very clear that it can fully confirm within a normal capability range. Therefore, in the present invention, the "polypeptide comprising a substantial part of the amino acid sequence as set forth in SEQ ID NO: 2", as defined above, delays the aging of a plant that a person skilled in the art can manufacture within the range of its usual capacity based on the disclosure herein. It is to be understood as meaning including all polypeptides in deleted form having functional and stress resistant functions.
  • polypeptide substantially similar to the polypeptides of (a) and (b) includes a function comprising one or more substituted amino acids, but comprising the amino acid sequence of SEQ ID NO: 2, ie aging of a plant. It refers to a polypeptide having delayed function and stress resistance function.
  • the degree of activity of the polypeptide or the degree of substitution of the amino acid is not a problem as long as the polypeptide containing at least one substituted amino acid retains the aging delay function and the stress resistance function of the plant.
  • a polypeptide comprising one or more substituted amino acids contains a large number of substituted amino acids, even if its activity is lower than that of a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, the polypeptide is aging of the plant. It is included in the present invention as long as it has a delay function and a stress tolerance function. Even if one or more amino acids are substituted, if the amino acid before substitution is chemically equivalent to the substituted amino acid, the polypeptide comprising such substituted amino acid will still retain the function of the original polypeptide.
  • the polypeptide having such substituted amino acid (s) may be It will still retain the function of the original polypeptide.
  • a negatively charged amino acid such as glutamic acid
  • another negatively charged amino acid such as aspartic acid
  • polypeptide having such substituted amino acid (s) will still retain the function of the original polypeptide even if its activity is low. will be.
  • polypeptides comprising amino acid (s) substituted at the N-terminal or C-terminal portion of the polypeptide will still retain the function of the original polypeptide.
  • Those skilled in the art can produce a polypeptide comprising at least one substituted amino acid as described above, but still retaining the aging delaying function and stress resistance function of the plant with the polypeptide comprising the amino acid sequence of SEQ ID NO: 2.
  • polypeptide comprising one or more substituted amino acids can also determine whether a polypeptide comprising one or more substituted amino acids still has this function.
  • the present specification discloses an example in which the base sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 are disclosed, and the polypeptide comprising the amino acid sequence of SEQ ID NO: 2 has an aging delay function and a stress resistance function of a plant. It is clear that the "polypeptide substantially similar to the polypeptide of (a) and (b)" of the present invention can be easily implemented by those skilled in the art.
  • polypeptide substantially similar to the polypeptide of (a) or (b) above should be understood as meaning including all polypeptides that contain one or more substituted amino acids but still have the aging delay and stress resistance functions of the plant.
  • polypeptide substantially similar to the polypeptide of (a) or (b) is meant to include all polypeptides that contain one or more substituted amino acids but still have the aging delay and stress resistance functions of plants, In view of the degree, the polypeptide is preferably higher in sequence homology with the amino acid sequence of SEQ ID NO.
  • the polypeptide has at least 60% sequence homology at the lower end of sequence homology, while at the upper limit of sequence homology, it is preferred that the polypeptide has 100% sequence homology. More specifically, the above sequence homology is 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90% , 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% in order of higher.
  • polypeptides substantially similar to the polypeptides of (a) and (b) above are not only “polypeptides substantially similar to polypeptides comprising the entire amino acid sequence of SEQ ID NO: 2", All descriptions above are intended to include “substantially similar to polypeptides comprising all of the amino acid sequence of SEQ ID NO: 2," as well as “amino acid sequence of SEQ ID NO: 2" as the polypeptides comprising substantially substantial The same applies to "polypeptides" that are substantially similar to polypeptides comprising a substantial portion of.
  • polypeptide as described above refers to a polypeptide comprising the entire amino acid sequence of SEQ ID NO: 2, having a substantial portion of the amino acid sequence of SEQ ID NO: 2, having a aging delaying function and a stress resistance function of a plant, and In addition to including polypeptides substantially similar to the above polypeptides, it is meant to include all polypeptides of the preferred embodiments as described above.
  • the polynucleotide of the present invention is substantially isolated from the isolated polynucleotide and the polypeptides having the aging delay function and the stress resistance function of the plant, and encoding the polypeptide including all or a substantial part of the amino acid sequence described in SEQ ID NO: 2.
  • Encoding an isolated polypeptide includes an isolated polynucleotide, and furthermore, in a preferred embodiment, an isolated polynucleotide encoding all polypeptides having the aging control function of the plant and having the sequence homology in the order of sequence homology as described above. Include. When amino acid sequences are found, those skilled in the art can readily prepare polynucleotides encoding such amino acid sequences based on those amino acid sequences.
  • isolated polynucleotide herein includes both chemically synthesized polynucleotides, polynucleotides isolated from organisms, especially Arabidopsis thaliana , and polynucleotides containing modified nucleotides, and are either single stranded or double stranded. It is defined as including all polymers of stranded RNA or DNA.
  • the present invention relates to a method for producing a plant with delayed aging.
  • the method for producing a delayed aging plant of the present invention comprises the steps of: (a) overexpressing a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to the nucleotide sequence of SEQ ID NO: 1 in the plant; and (b) a phenotype of delayed aging. It comprises a step of selecting a plant having a.
  • the term "aging delay” refers to a property of prolonged plant life compared to wild-type plants, and specifically, the yellowing and / or necrosis of leaves and / or stems is delayed compared to wild-type plants, or the chlorophyll content of plants is wild-type. It is more characteristic than plants or photosynthetic efficiency of plants is higher than wild type plants.
  • plant is meant to include mature plants, immature plants (plants), plant seeds, plant cells, plant tissues and the like.
  • plant cells or plant tissues are described in European Patent EP0116718, European Patent EP0270822, International Patent WO 84/02913, Gould et al. 1991, Plant Physiol 95,426-434, can be used to develop and grow into mature plants.
  • plant includes all plants for which delayed aging can give useful results to humans.
  • the delay in aging is directly related to an increase in production, i.e. an increase in seed productivity and / or biomass size of the individual, so in the sense of the plant, the increase in production is primarily useful for human crops such as rice, wheat, barley, corn, soybeans, potatoes, and red beans.
  • gene consisting of a sequence similar to the nucleotide sequence of SEQ ID NO: 1 is a gene encoding the first amino acid of SEQ ID NO: 2 while having a base sequence different from that of the gene of SEQ ID NO: 1 due to codon degeneracy
  • genes consisting of the nucleotide sequence of SEQ ID NO: 1 all of the nucleotide sequence of SEQ ID NO: 1 and other nucleotide sequences due to the evolutionary pathways different according to the type of plant, having the aging delay function of the plant It is meant to include genes.
  • the gene consisting of a sequence similar to the nucleotide sequence of SEQ ID NO: 1 is preferably higher in sequence homology with the nucleotide sequence of SEQ ID NO: 1, and most preferably, having 100% sequence homology.
  • the gene has a sequence homology of 60% or more with the nucleotide sequence of SEQ ID NO: 1.
  • sequence homology is 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73 %, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, Higher in order of 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99% is preferred.
  • overexpression means expression above the level expressed in wild-type plants. Such “overexpression” can be determined directly by quantifying the gene of SEQ ID NO: 1 or a gene consisting of a sequence similar to the nucleotide sequence of SEQ ID NO: 1 or indirectly quantified by quantifying the protein encoded by the gene.
  • the step (a) may be performed by a genetic engineering method.
  • Genetic engineering method includes the steps of: (i) inserting a gene having the sequence of SEQ ID NO: 1 or a sequence similar to the sequence of SEQ ID NO: 1 into an expression vector to be operably linked to a regulatory sequence capable of overexpressing it, and ( ii) transforming the expression vector into a plant.
  • operably means that the transcription and / or translation of a gene is linked to be affected. For example, if a promoter influences the transcription of a gene linked to it, the promoter and the gene are operably linked.
  • regulatory sequence is meant to include all sequences whose presence may affect the transcription and / or translation of a gene linked thereto, and such regulatory sequences include a promoter sequence and a polyadenylation signal. ), The replication start point.
  • promoter follows the conventional meaning known in the art, specifically located upstream (5 'side) based on the transcription initiation point of a gene and binding to DNA-dependent RNA polymerase.
  • nucleic acid sequences having the function of controlling transcription of one or more genes including sites, transcriptional initiation sites, transcription factor binding sites, and the like.
  • Such a promoter may be a TATA box upstream of the transcription initiation point (usually at the transcription initiation point (+1) -20 to -30 position), CAAT box (usually approximately -75 position relative to the transcription initiation site if it is of eukaryotes) Present), a 5 'enhancer, a transcription repression factor, and the like.
  • Usable promoters include constitutive promoters (promoters which induce constant expression in all plant tissues), inducible promoters (expression of target genes in response to specific external stimuli) as long as they are promoters capable of overexpressing the gene of SEQ ID NO. 1 linked thereto. Promoters that induce expression or promoters that specifically induce expression in specific developmental periods or specific tissues).
  • constitutive promoters include the promoter of the 35S RNA gene of cauliflower mosaic virus (CaMV), and the ubiquitin family of promoters (Christensen et al., 1992, Plant Mol). Biol. 18, 675-689; EP0342926; Cornejo et al., 1993, Plant Mol. Biol.
  • rice actin promoter Zhang et al. 1991, The Plant Cell 3, 1155-1165
  • rice actin promoter Zhang et al. 1991, The Plant Cell 3, 1155-1165
  • inducible promoters include the yeast metallothionein promoter (Mett et al., Proc. Natl. Acad. Sci., USA, 90: 4567, 1993), which is activated by copper ions, substituted by substituted benzenesulfonamides.
  • In2-1 and In2-2 promoters (Hershey et al., Plant Mol. Biol., 17: 679, 1991), GRE regulatory sequences regulated by glucocorticoids (Schena et al., Proc. Natl. Acad.
  • Transcription termination sequence poly (A ) Addition signal (polyadenylation) signal As a sequence acting as), it is to enhance the completeness and efficiency of transcription.
  • transcription termination sequences include the transcription termination sequence of the nopaline synthase (NOS) gene, the transcription termination sequence of the rice ⁇ -amylase RAmy1 A gene, and the transcription termination of the Octopine gene of Agrobacterium tumefaciens.
  • the expression vector may include a selection marker gene.
  • Marker gene as used herein means a gene encoding a trait that enables the selection of a plant or plant cell comprising such a marker gene.
  • the marker gene may be an antibiotic resistance gene or may be a herbicide resistance gene.
  • suitable selectable marker genes include genes of adenosine deaminase, genes of dihydrofolate reductase, genes of hygromycin-B-phosphortransferase, genes of thymidine kinase, genes of xanthine-guanine phosphoribosyltransfer Laze gene, phosphinnothricin acetyltransferase gene, etc. are mentioned.
  • the term "transformation” refers to a modification of the genotype of a host plant by the introduction of a hereditary gene, and regardless of the method used for the transformation, the herb gene is a host plant, more precisely a cell of the host plant. Introduced into and integrated into the genome of a cell.
  • the hereditary genes include homologous and heterologous genes, wherein “homologous genes” refer to endogenous genes of a host organism or the same species, and “heterologous genes” are genes that do not exist in the organism to which they are transformed.
  • Arabidopsis derived genes are homologous to Arabidopsis plants, but heterologous to tomato plants.
  • a method of transforming a plant with an exogenous gene may use a method known in the art, such as a direct gene transfer method using a gene gun, an in planta transformation method using a floral dip, pollen mediation, and the like. Transformation methods, protoplast transformation methods, viral mag transformation methods, liposome mediated transformation methods, and the like can be used. In addition, it is also possible to select and use a transformation method suitable for a specific plant, for example, a method for transforming corn is described in US Pat. No.
  • Generally used in transforming plants is a method of infecting seedlings, plant seeds and the like with the transformed Agrobacterium.
  • Such Agrobacterium mediated transformation methods are well known in the art (Chilton et al., 1977, Cell 11: 263: 271; European Patent EP 0116718; US Patent US 4,940,838), and methods suitable for particular plants are also known in the art.
  • the Agrobacterium mediated transformation method uses Ti-plasmid, which will contain left and right border sequences that allow the integration of T-DNA into the genome of plant cells.
  • the transformed plant is grown and grown to be visually selected through the degree of progress of leaf yellowing or the progression of leaf necrosis, or when the selection marker gene is transformed.
  • the selection may be performed using a selection marker gene, and further, may be selected through a method of quantifying chlorophyll content, photosynthetic efficiency, and the like, and a method of mixing the above methods.
  • the invention relates to a method of producing a stress resistant plant in another aspect.
  • the method of producing a stress resistant plant of the present invention comprises the steps of: (a) overexpressing a gene having the nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to the nucleotide sequence of SEQ ID NO: 1 in the plant, and (b) having a stress resistant phenotype Comprising plant screening.
  • stress means oxidative stress or drought stress.
  • Step (a) may be carried out genetically, as described above with respect to the method for producing a aging delayed plant of the present invention for this genetic engineering method.
  • Step (b) may be performed with the naked eye through the progress of leaf yellowing or the progression of leaf necrosis, or when the selection marker gene is transformed together during transformation, the screening may be performed using the selection marker gene.
  • the chlorophyll content or the content of active oxygen species such as H 2 O 2 can be selected through a method of quantifying, a method of mixing the above methods.
  • the present invention relates to a method for producing a plant having a production increasing characteristic of the present invention.
  • the method for producing a plant having a production increasing characteristic of the present invention comprises the steps of: (a) overexpressing a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to the nucleotide sequence of SEQ ID NO: 1 in the plant, and (b) increasing the yield And selecting the plant having the characteristic.
  • the term " increase in production characteristics" means that the biomass (size and / or mass) of the whole, stem, root and / or leaves of the plant has increased compared to the wild type plant and / or the seed productivity of the plant is increased in the wild type plant. Compared to increased characteristics.
  • Step (a) may be carried out genetically, as described above with respect to the method for producing a aging delayed plant of the present invention for this genetic engineering method.
  • Step (b) may be performed by comparing the biomass size of the plants with the naked eye, or when the selection marker gene is transformed together at the time of transformation, using the selection marker gene, or counting the number of seeds It can be measured. Moreover, these methods can also be mixed and measured.
  • the present invention relates to a method for delaying aging of a plant of the present invention.
  • the method for delaying aging of a plant of the present invention is to (a) operably link a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to that of SEQ ID NO: 1 to a regulatory sequence capable of overexpressing it. Inserting into the expression vector and (b) transforming the expression vector into a plant.
  • the present invention relates to a method for increasing the resistance to stress of the plant of the present invention.
  • the method of increasing the resistance to stress of the plant of the present invention is operable to (a) a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to that of SEQ ID NO: 1 to a regulatory sequence capable of overexpressing it Inserting it into an expression vector so as to be linked to each other and (b) transforming the expression vector into a plant.
  • the present invention relates to a method for increasing the yield of the plant of the present invention.
  • the method of increasing the yield of the plant of the present invention is to (a) operably linked to a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to that of SEQ ID NO: 1 to a regulatory sequence capable of overexpressing it Inserting into the expression vector and (b) transforming the expression vector into a plant.
  • Steps (a) and (b) in the above methods are the same as those described in connection with the method for producing the delayed aging plant of the present invention.
  • the present invention is the delayed aging of the gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to the nucleotide sequence of SEQ ID NO: 1 obtained by the method for producing an aging delayed plant of the present invention
  • the present invention relates to a transgenic plant having characteristics.
  • the plant is a transgenic plant having delayed aging characteristics by being introduced into a gene encoding the ATGH1 protein consisting of the amino acid sequence of SEQ ID NO: 2, in particular, a gene having the nucleotide sequence of SEQ ID NO: 1 and overexpressed.
  • the present invention is a stress resistance overexpressed a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to the nucleotide sequence of SEQ ID NO: 1 obtained by the method for producing a stress resistant plant of the present invention It relates to a transgenic plant having a.
  • the plant is a transgenic plant that is stress-resistant by introducing and overexpressing a gene encoding the ATGH1 protein consisting of the amino acid sequence of SEQ ID NO: 2, in particular, a gene having the nucleotide sequence of SEQ ID NO: 1.
  • the present invention is an overexpression of a gene having a nucleotide sequence of SEQ ID NO: 1 or a gene having a sequence similar to the nucleotide sequence of SEQ ID NO: 1 obtained by the method for producing a plant having a production increase characteristic of the present invention
  • the present invention relates to a transgenic plant having increased production yield characteristics.
  • the plant is a transgenic plant having an increase in production characteristics by introducing the gene encoding the ATGH1 protein consisting of the amino acid sequence of SEQ ID NO: 2, in particular the gene ATGH1 having the nucleotide sequence of SEQ ID NO: 1, is overexpressed.
  • the genome is not only when the gene is introduced and transformed into plant cells, plant tissues, or plant seeds capable of developing and growing into the “transforming plant” mature plant, but also by crossing with the transformed plant. This includes modified plants, plant seeds, plant cells.
  • the ATHG1 protein and its gene having delayed senescence and stress resistance functions of plants. Since the gene has a delaying aging function and a stress resistance function, when transforming a plant with this gene, the gene may be delayed in aging, increasing the productivity of the plant, and being resistant to stress.
  • Figure 1 shows the structure (schematic) of the pCSEN-ATHG1 recombinant vector in which the ATHG1 gene having a aging delay function and stress resistance function of the plant is introduced in the sense direction.
  • Figure 2 is a photograph of the Arabidopsis grown 60 days after germination of the Arabidopsis T 1 plants transformed with the pCSEN-ATHG1 recombinant vector of FIG.
  • ATHG1 ox-a Arabidopsis T 1 plant transformed with pCSEN-ATHG1 recombinant vector
  • ATHG1 ox-b Arabidopsis T 1 plant transformed with pCSEN-ATHG1 recombinant vector
  • Figure 3 is a photograph of the Arabidopsis grown 60 days after germination of the Arabidopsis T 2 line transformed with the pCSEN-ATHG1 recombinant vector of FIG.
  • ATHG1 ox-5 Arabidopsis T 2 line transformed with pCSEN-ATHG1 recombinant vector
  • ATHG1 ox-6 Arabidopsis T 2 line transformed with pCSEN-ATHG1 recombinant vector
  • ATHG1 ox-5 Arabidopsis T 2 line transformed with pCSEN-ATHG1 recombinant vector
  • ATHG1 ox-6 Arabidopsis T 2 line transformed with pCSEN-ATHG1 recombinant vector
  • Figure 5 shows the results of analyzing the ATGH1 gene expression patterns of Arabidopsis wild-type (Col-0), delayed aging-induced mutant ATHG1 ox-5 , and ATHG1 ox-6 grown for 25 days after germination through RT-PCR.
  • ACT8 is a PCR positive control.
  • FIG. 6 shows 60 days every 5 days for the Arabidopsis wild-type (Col-0), delayed aging-induced mutant ATHG1 ox-5 , and 3-4 leaves of ATHG1 ox-6 from 25 days after germination This picture shows the phenotype of the leaf.
  • Figure 7 shows the chlorophyll of the leaves from day 25 after germination to the left lobe of Arabidopsis wild-type (Col-0), mutant ATHG1 ox-5 , and ATHG1 ox-6 3-4 leaves every 60 days It is a figure that investigated the content.
  • FIG. 8 shows photosynthesis of leaves from the 25th day after germination of the Arabidopsis wild-type (Col-0), the delayed aging-induced variant ATHG1 ox-5 , and the left lobe 3-4 of ATHG1 ox-6 up to 60 days every 5 days.
  • the figure shows the efficiency in Fv / Fm.
  • Figure 9 shows the aging of the leaves of the Arabidopsis wild type (Col-0), delayed aging-induced variants ATHG1 ox-5 , and ATHG1 ox-6 from day 25 after germination until 60 days every 5 days.
  • Expression of the marker gene was analyzed by qRT-PCR, and ACT8 is a PCR positive control.
  • CAB2 is a chlorophyll a / b binding protein gene
  • SEN4 and SAG12 are aging genes and aging marker genes.
  • FIG. 10 shows detachment of the Arabidopsis wild-type (Col-0), delayed aging-induced mutant ATHG1 ox-5 , and ATHG1 ox-6 left and right leaves 3-4 days after germination to maintain cancer every 2 days.
  • Figure 14 shows the phenotype of the leaves.
  • FIG. 11 shows detachment of the Arabidopsis wild-type (Col-0), delayed aging-induced mutant ATHG1 ox-5 , and ATHG1 ox-6 left and right leaves 3-4 days after germination to maintain cancer every 2 days.
  • Figure 14 shows the chlorophyll content in leaves.
  • FIG. 13 shows detachment of the Arabidopsis wild species (Col-0), delayed senescence-induced mutant ATHG1 ox-5 , and ATHG1 ox-6 3-4 left lobes at 25 days after germination to maintain cancer status every 2 days.
  • the expression of senescence marker genes in leaves up to 14 days was analyzed by qRT-PCR, and ACT8 is a PCR positive control.
  • CAB2, SEN4, and SAG12 are aging marker genes.
  • FIG. 15 is an enlarged view of the 6-day TB staining and the 4-day DAB staining of the Arabidopsis wild-type (Col-0) and the delayed-induced mutant ATHG1 ox-5 of FIG.
  • FIG. 16 shows the treatment of H 2 O 2 with detached left lobe of Arabidopsis wild-type (Col-0), delayed senescence induced ATHG1 ox-5 , and ATHG1 ox-6 3-4 days after germination. This figure shows a change in the phenotype of a leaf. Mock treatment is a control
  • FIG. 17 shows detached Arabidopsis wild species (Col-0), delayed aging-induced mutant ATHG1 ox-5 , and ATHG1 ox-6 3-4 left lobes treated with H 2 O 2 on day 25 after germination.
  • Figure shows changes in chlorophyll content in leaves.
  • FIG. 19 shows drought treatment of Arabidopsis wild-type (Col-0), delayed aging-induced mutants ATHG1 ox-5 , and ATHG1 ox-6 for 13 days after germination , and changes in leaf weight that occurred during the day.
  • Col-0 Arabidopsis wild-type
  • ATHG1 ox-5 delayed aging-induced mutants
  • ATHG1 ox-6 ATHG1 ox-6
  • Figure 20 shows the migration of ATHG1-GFP fusion proteins into the nucleus of Arabidopsis protoplasts.
  • GFP control Photo under fluorescence microscopy of 35S-GFP positive control
  • ATHG1-GFP Photographed Under Fluorescence Microscopy of ATHG1-GFP
  • 21 is a diagram confirming the migration of Arabidopsis protoplasts to the nucleus of the ATHG1 :: GFP fusion protein.
  • DAPI Fluorescence micrograph of nuclear staining of Arabidopsis protoplasts
  • Example 1 It has the function of delaying the aging of the plant from the Arabidopsis and the stress tolerance ATHG1 Isolation of genes
  • Arabidopsis cultivars were grown in pots containing soil or Petri dishes containing MS (Murashige and Skoog salts, Sigma, USA) medium containing 2% sucrose (pH 5.7) and 0.8% agar. . When cultivated in a pollen, it was grown in a growth chamber controlled at a light cycle of 16/8 hours at a temperature of 22 ° C.
  • the forward primer (BglII / AT4G17800 SOE), which is represented by SEQ ID NO: 3 and contains the sequence of restriction enzyme BglII -F, 5'-AGA TCT ATG GCT GGT CTT GAT CTA GGC A-3 ') and reverse primer (BstEII / AT4G17800 SOE-R, 5'-GGT), represented by SEQ ID NO: 4 and containing the sequence of restriction enzyme BstEII GAC CTC AGA AAG GAC CTC TTC CAC CG-3 ').
  • the two primers were used to amplify and isolate full-length cDNA from polymerase chain reaction (PCR) from Arabidopsis cDNA prepared in Example 1-2.
  • the isolated cDNA As a result of analysis of the isolated cDNA, it has a 879 bp transcriptional translation frame (ORF) encoding 292 amino acids having a molecular weight of about 29.7 kDa, it was confirmed that it consists of one exon, AT- it has the hook's domain ATHG1 - was named (h AT ook protein of G enomine 1).
  • the isoelectric point of the ATHG1 protein encoded by the gene was 6.41 (hereinafter, the gene is called " ATHG1 " or " ATHG1 gene” using italics, and the protein is called “ATHG1" or "ATHG1 protein”).
  • a transgenic Arabidopsis in which the ATHG1 gene was introduced in the sense direction was prepared to change the expression of the ATHG transcript.
  • ATHG1 cDNA was amplified by PCR from Arabidopsis cDNA using a forward primer represented by SEQ ID NO: 3 and containing a sequence of restriction enzyme BglII and a reverse primer represented by SEQ ID NO: 4 and containing a sequence of restriction enzyme BstEII .
  • the DNA was digested with restriction enzymes BglII and BstEII and cloned in the sense direction into a pCSEN vector prepared to be controlled by the SEN1 promoter, an inducible promoter, to construct a pCSEN-ATHG1 recombinant vector, which is a sense construct for the ATHG1 gene.
  • the SEN1 promoter has specificity for the gene expressed according to the growth stage of the plant.
  • FIG. 1 is a diagram showing a pCSEN-ATHG1 recombinant vector in which the ATHG1 gene is introduced in the sense direction into the pCSEN vector.
  • BAR indicates a phosphinothricin acetyltransferase gene ( BAR gene) that confers resistance to a Vaster herbicide
  • RB is a right border
  • LB is a left border
  • P35S is a CaMV 35S promoter
  • PSEN is the SEN1 promoter
  • Nos-A refers to the polyA of the nopaline synthase gene.
  • the pCSEN-ATHG1 recombinant vector was introduced into Agrobacterium tumefaciens using an electroporation method.
  • the transformed Agrobacterium cultures were incubated at 28 ° C. until the OD 600 value was 1.0, and the cells were harvested by centrifugation at 25 ° C. at 5,000 rpm for 10 minutes.
  • Harvested cells were suspended in Infiltration Medium (IM; 1X MS SALTS, 1X B5 vitamin, 5% sucrose, 0.005% Silwet L-77, Lehle Seed, USA) medium until the final OD 600 value was 2.0.
  • IM Infiltration Medium
  • the Arabidopsis was placed in a polyethylene bag for 24 hours. Thereafter, the transformed Arabidopsis cultivars continued to grow to harvest seeds (T 1 ).
  • T 1 a non-transformed wild type Arabidopsis or a Arabidopsis transformed with only a vector (pCSEN vector) containing no ATHG1 gene was used.
  • Seeds harvested from the transformed Arabidopsis larvae as in ⁇ Example 2-1> were selected by immersing and incubating for 30 minutes in a 0.1% Bassta herbicide (light, South Korea) solution. Thereafter, the pollen was treated 5 times with the Basta herbicide during the growth of the transformed Arabidopsis larvae, and the Arabidopsis growth change in each pollen was investigated.
  • T 1 Arabidopsis transformed with pCSEN-ATHG1 vector ATHG1 ox-a and -b were control (transgenic Arabidopsis or wild-type Arabidopsis transformed only with vectors containing no ATHG1 gene (pCSEN vector)) and their germination 65 days after germination
  • the ATHG1 ox-a and -b variants surprisingly had distinct phenotypic characteristics of aging delay, and the difference in the degree of this aging delay in transgenic individuals differs between gene overexpressions. It is judged to be due (FIG. 2).
  • the phenotypes of these lines were examined by receiving T 2 transgenic seeds from the T 1 transgenic Arabidopsis.
  • T 2 transformed Arabidopsis thawed for 3 days at low temperature (4 °C) T 2 transformed seedlings were grown in pollen, and transgenic Arabidopsis was selected through the treatment of Basta herbicide. Phenotyping of selected Arabidopsis T 2 transformation lines was performed 50 days after germination (data not shown) and 60 days (FIG. 3).
  • pCSEN-ATHG1 ATHG1 ox-5 and ATHG1 ox-6 mutant lines with a construct was shown when compared with Arabidopsis wild-type (Col-0), is just as T 1 variants aging delay plant development clearly, these The aging delay was slightly different from line to line, which is attributed to the fact that gene overexpression is slightly different from line to line. In addition, these variants resulted in not only a delayed aging phenotype but also a marked increase in individual size and seed yield as shown below during aging delay.
  • RNAsey Plant Mini Kit (QIAGEN, Germany) was used to analyze the expression patterns of ATHG1 genes of mutants with delayed aging phenotypes from leaves of ATHG1 ox-5 and ATHG1 ox-6 mutants grown for 25 days after germination. Total RNA was extracted respectively. 1 ⁇ g of RNA each as a template and 5 minutes at 65 ° C. using Superscript III Reverse Tanscriptase (INVITROGEN, USA); 60 minutes at 50 ° C; And cDNA was synthesized at 70 ° C. for 15 minutes.
  • PCR was performed using the primers specific to the following Table 1 for the ATHG1 gene and the ACT8 gene used as a PCR positive control.
  • PCR denatured template DNA by heating at 94 ° C. for 2 minutes and then 1 minute at 94 ° C .; 1 minute 30 seconds at 55 ° C; And 1 cycle at 72 °C 1 cycle was carried out a total of 30 times, followed by a final reaction for 15 minutes at 72 °C.
  • the PCR product was confirmed by 1% agarose gel electrophoresis, the results are shown in FIG.
  • Example 3-1 Phenotypic Changes in Leaves According to Age-Dependent Aging of ATHG1 Overexpressing Variants
  • the phenotype of the leaves was observed from the left lobe 3-4 times every 60 days until 60 days after germination.
  • the yellowing of the leaves rapidly appeared after 40 days, and the leaves entered the necrosis state from the 50th day.
  • the yellowing of the leaves progressed after 55 days and the necrosis of the leaves was hardly occurring even on the 60th day (FIG. 6). This fact suggests that the ATHG1 gene may play an important role in delaying plant aging.
  • Example 3-2 Changes in Chlorophyll Content with Age-Dependent Aging of ATHG1 Overexpressing Variants
  • Chlorophyll was extracted from each sample leaf using 80% (V / V) acetone to measure chlorophyll content. Chlorophyll content was measured according to the method of Lichtenthaler and Wellburn ( Biochemical Society Transduction 603: 591 ⁇ 592, 1983) using extinction coefficients of 663.2 nm and 664.8 nm. As a result, as shown in Figure 7, the chlorophyll content in the wild species showed a sharp decrease after 40 days after germination and the chlorophyll content was 0% on day 55, but in the case of ATHG1 ox-5 and -6 55 after germination When work was confirmed that the chlorophyll content of more than 40% of the initial measurement was shown.
  • Photosynthetic efficiency was measured using Oh et al . ( Plant Mol. Biol. 30: 939, 1996). First, the leaves of each DAG (day after germination) were treated with cancer for 15 minutes, and then the fluorescence of chlorophyll was measured using a Plant Efficiency Analyzer (Hansatech). Photosynthetic efficiency was expressed by the photochemical efficiency of PSII (photosystem II) using chlorophyll fluorescence properties, which was the maximum variable fluorescence (Fv) versus the maximum value of fluorescence (Fm). It is expressed as the ratio of (Fv / Fm). Higher values indicate better photosynthetic efficiency.
  • Example 3-4 Expression Changes of Aging-Related Genes According to Age-Dependent Aging of ATHG1 Overexpressing Variants
  • Quantitative analysis of marker genes for aging was confirmed by Quantitative real-time PCR (qRT-PCR) using Applied Bio-systems' 7300 Real Time PCR System. Aging marker gene was used as a SAG12, SEN4 and CAB2 genes, as qRT-PCR was used as a positive control ACT8 gene.
  • the primers used are shown in Table 2 below.
  • Drought tolerance analysis of the overexpressed variants of ATHG1 drought the 13-day-old plants after thirteen days of drought, and compared the phenotypic changes of the entire plant with the weight change of leaves per plant during the drought. The degree of resistance was confirmed. As a result, it was found that the yellowing of the leaf type rapidly progressed due to drought, and the weight of the leaf was markedly reduced to less than 30% of the initial state by drought. In contrast, the overexpressing variant of ATHG1 was still undergoing greening of the leaves even during drought treatment, and the weight loss of the leaves caused little by little drought treatment (Figs. 18 and 19). This implies that ATHG1 provides maximum plant moisture retention even under drought stress, providing resistance to drought stress.
  • motifs are motifs found in transcriptional regulators or transcription factors that regulate the expression of other genes in the nucleus. Therefore, the inventors confirmed whether the ATHG1 protein of the present invention migrates to the nucleus.
  • the ATHG1 gene was prepared using a primer containing a Psd I and Stu I restriction enzyme (forward primer, SEQ ID NO: 17, ATHG1 F: 5'-CTGCAGATGGCTGGTCTTGATCTAGGCA-3 'reverse primer, SEQ ID NO: 18, ATHG1 R: 5'-AGGCCTGAAAGGACCTCTTCCACCGGAA-3) was amplified by PCR using ').
  • the amplified cDNA was digested with Psd I and Stu I restriction enzymes, tagged with GFP (green fluorescence protein) and inserted into the plasmid to prepare plasmid pATHG1-GFP vectors.
  • the pATHG1-GFP vector was transfected into Arabidopsis protoplasts and observed using a fluorescence microscope, and the nuclear position image was stained with DAPI (0.5ug / ul). It was confirmed by the method (Paulus M Fong et al .; Cell Res. 16: 479-488, 2006).

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne une protéine ATHG1 à fonction de retardement de sénescence et à fonction de résistance au stress d'une plante, ainsi qu'un gène et une utilisation de la protéine. Une plante transformée avec ledit gène retarde la sénescence de la plante, présente un rendement accru de production, et induit une résistance de la plante au stress environnemental, au stress oxydatif et au stress de la sécheresse notamment.
PCT/KR2010/000767 2010-02-08 2010-02-08 Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine WO2011096609A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR2010/000767 WO2011096609A1 (fr) 2010-02-08 2010-02-08 Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2010/000767 WO2011096609A1 (fr) 2010-02-08 2010-02-08 Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine

Publications (1)

Publication Number Publication Date
WO2011096609A1 true WO2011096609A1 (fr) 2011-08-11

Family

ID=44355597

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/000767 WO2011096609A1 (fr) 2010-02-08 2010-02-08 Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine

Country Status (1)

Country Link
WO (1) WO2011096609A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664446B2 (en) * 1999-03-23 2003-12-16 Mendel Biotechnology, Inc. Transgenic plants comprising polynucleotides encoding transcription factors that confer disease tolerance
US6717034B2 (en) * 2001-03-30 2004-04-06 Mendel Biotechnology, Inc. Method for modifying plant biomass
WO2004035798A2 (fr) * 2002-10-18 2004-04-29 Cropdesign N.V. Identification de nouveaux genes cibles e2f et leur utilisation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664446B2 (en) * 1999-03-23 2003-12-16 Mendel Biotechnology, Inc. Transgenic plants comprising polynucleotides encoding transcription factors that confer disease tolerance
US6717034B2 (en) * 2001-03-30 2004-04-06 Mendel Biotechnology, Inc. Method for modifying plant biomass
WO2004035798A2 (fr) * 2002-10-18 2004-04-29 Cropdesign N.V. Identification de nouveaux genes cibles e2f et leur utilisation

Similar Documents

Publication Publication Date Title
JP4021615B2 (ja) 植物成長及び発達の遺伝子制御
WO2012148121A2 (fr) Protéine atpg7 conférant à une plante des caractéristiques d'augmentation du rendement, de retardement du vieillissement et de résistance au stress, gène de ladite protéine et utilisation de celle-ci
KR101850003B1 (ko) 식물의 노화 지연 기능 및 스트레스 내성 기능을 갖는 ａｔｈｇ１ 단백질과 그 유전자 및 이들의 용도
WO2011090272A2 (fr) Gène osmpt modifiant l'architecture d'une plante (forme de la plante) et améliorant son rendement, utilisation correspondante
WO2013062327A2 (fr) Protéine atpg4 ayant une fonction d'augmentation de la productivité, une fonction antivieillissement, une fonction de résistance au stress de plantes, gènes associés et utilisations associées
WO2010095910A2 (fr) Polypeptide ayant une fonction permettant de retarder l'anthèse ou de supprimer la croissance, polynucléotide codant pour ce polypeptide et son utilisation
WO2018066897A2 (fr) Protéine mtatpg1 ayant une fonction d'augmentation de la productivité et de retardement de la sénescence chez une plante, son gène, et utilisation de cette protéine et de son gène
WO2014209060A1 (fr) Protéine atpg6 et son gène, ayant des fonctions d'augmentation du rendement des plantes, de résistance au stress et de retard la sénescence, et son utilisation
WO2014209036A1 (fr) Protéine atpg10 et son gène, ayant des fonctions d'augmentation du rendement des plantes, de résistance au stress et de retard la sénescence, et son utilisation
WO2013125835A1 (fr) Protéine atpg3 ayant une capacité à augmenter la productivité, une capacité à retarder la sénescence et une capacité à conférer aux plantes une résistance au stress, gène de la protéine atpg3 et leur utilisation
WO2012148122A2 (fr) Protéine atpg8 conférant à une plante des caractéristiques d'augmentation du rendement, de retardement du vieillissement et de résistance au stress, gène de ladite protéine et utilisation de celle-ci
KR20090049555A (ko) 식물 왜화 유도 기능을 가진 폴리펩티드, 이를 암호화하는 폴리뉴클레오티드 및 이들의 용도
Peng et al. Increasing branch and seed yield through heterologous expression of the novel rice S-acyl transferase gene OsPAT15 in Brassica napus L.
WO2011096609A1 (fr) Protéine athg1 à fonction de retardement de la sénescence et à fonction de résistance au stress d'une plante, et gène et utilisation de la protéine
KR101957736B1 (ko) 식물의 고온 스트레스 내성을 증가시키는 벼 유래 OsOPT10 유전자 및 이의 용도
CN101023096B (zh) 调节植物叶片寿命的蛋白质，其基因和它们的用途
WO2011122761A2 (fr) Protéine gsdl2 comportant une fonction d'extension de vie pour les végétaux et une fonction de résistance au stress pour les végétaux, son gène et ses applications
WO2018066898A2 (fr) Protéine mtatpg2 à fonction d'augmentation de productivité, améliorant la résistance au stress et retardant la sénescence d'une plante, gène associé, et utilisation de ladite protéine et dudit gène
KR101592357B1 (ko) 식물의 냉해 스트레스 내성과 관련된 신규 유전자 및 그의 용도
KR20140032694A (ko) 내염성을 증가시키는 벼 유래의 ＯｓＭＬＤ 유전자 및 이의 용도
KR102231138B1 (ko) 식물의 염 스트레스 내성을 조절하는 벼 유래의 OsXCP2-5 유전자 및 이의 용도
KR102231136B1 (ko) 식물의 염 스트레스 내성을 조절하는 벼 유래의 OsCP1 유전자 및 이의 용도
KR101190272B1 (ko) 벼 유래의 ＯｓＺＩＰ1 유전자 및 단백질
KR20190068282A (ko) 식물의 생산성 증대 기능, 노화 지연 기능 및 스트레스 내성 기능을 갖는 ahl26 단백질과 그 유전자 및 이들의 용도
KR101648559B1 (ko) 스트레스 유래 비정상적 단백질 제거를 통한 식물 스트레스 내성과 관련된 신규 유전자 및 그의 용도

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10845296

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10845296

Country of ref document: EP

Kind code of ref document: A1