WO2011071880A1 - Fiber-reinforced swing bucket centrifuge rotor and related methods - Google Patents

Fiber-reinforced swing bucket centrifuge rotor and related methods Download PDF

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Publication number
WO2011071880A1
WO2011071880A1 PCT/US2010/059231 US2010059231W WO2011071880A1 WO 2011071880 A1 WO2011071880 A1 WO 2011071880A1 US 2010059231 W US2010059231 W US 2010059231W WO 2011071880 A1 WO2011071880 A1 WO 2011071880A1
Authority
WO
WIPO (PCT)
Prior art keywords
rotor
bucket
straps
strap
pair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/059231
Other languages
English (en)
French (fr)
Inventor
Sina Piramoon
Alireza Piramoon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fiberlite Centrifuge LLC
Original Assignee
Fiberlite Centrifuge LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fiberlite Centrifuge LLC filed Critical Fiberlite Centrifuge LLC
Priority to JP2012543197A priority Critical patent/JP5728491B2/ja
Priority to CN201080055353.1A priority patent/CN102712001B/zh
Priority to DE112010004713.8T priority patent/DE112010004713B4/de
Priority to GB1209783.8A priority patent/GB2488476B/en
Publication of WO2011071880A1 publication Critical patent/WO2011071880A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/085—Rotary bowls fibre- or metal-reinforced
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
    • B04B9/08—Arrangement or disposition of transmission gearing ; Couplings; Brakes
    • B04B2009/085—Locking means between drive shaft and rotor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49826—Assembling or joining

Definitions

  • This invention relates generally to centrifuge rotors and, more particularly, to high-speed centrifuge rotors to be used with swing buckets.
  • Centrifuge rotors are typically used in laboratory centrifuges to hold samples during centrifugation. While centrifuge rotors may vary significantly in construction and in size, one common rotor structure is a swing bucket rotor having a solid rotor body defining an outer rim or wall of the rotor, and a plurality of wells or bays in a number such as two, four, or six for example, distributed radially within the rotor body and arranged symmetrically about an axis of rotation.
  • the presence of the outer rim or wall provides structural rigidity to the rotor, especially in view of the high dynamic forces experienced during centrifugation.
  • Buckets are placed in the wells, and are configured to hold sample tubes or similar laboratory-type containers, each containing a particular fluid material. During high-speed rotation, the buckets are permitted to swing within the wells, with the attained generally horizontal orientation of the buckets facilitating radially outward movement of the material held in the tubes.
  • One conventional type of swing bucket centrifuge rotor includes a generally metallic rotor configured to support an even number of swing buckets, such as four, six, or eight, for example, on diametrically opposite sides of the rotational axis of the rotor.
  • centrifuge rotors heretofore known for use for centrifugation. While the invention will be discussed in connection with certain embodiments, it will be understood that the invention is not limited to these embodiments. On the contrary, the invention includes all alternatives, modifications, and equivalents as may be included within the spirit and scope of the invention.
  • a centrifuge rotor having a rotor core that defines a rotational axis of the rotor.
  • a plurality of bucket supports is arranged about the axis of rotation.
  • the rotor includes first and second straps.
  • the first strap extends around a first pair of diametrically-opposed ones of the bucket supports for restricting outward movement of the first pair of bucket supports relative to the rotor core.
  • the second strap extends around a second pair of diametrically-opposed ones of the bucket supports for restricting outward movement of the second pair of bucket supports relative to the rotor core.
  • the first and second straps intersect one another at a location through the axis of rotation of the rotor.
  • the rotor may include a plurality of elongate arms extending from a central portion of the rotor core, with each of the bucket supports being located at a longitudinal end of one of the elongate arms.
  • the rotor may be such that each bucket support has first and second trunnions, with each of the trunnions being respectively configured to support a bucket, and with each of the bucket supports defining an outer perimeter of the rotor.
  • the first and second straps may be made of a high tensile-strength fiber material.
  • the first and second straps may be made of carbon fiber, an aramid fiber, a polyolefin fiber, or the like.
  • the first and second straps may be a composite material in which the fibers are encapsulated in a resin, such as a thermoplastic resin or a thermosetting resin.
  • a composite of carbon fibers in a thermosetting material is only an example.
  • the first strap may define a first loop and the second strap may define a second loop, with the second loop being larger than the first loop.
  • the first strap is located completely within the second loop at the location of intersection of the first and second straps with one another.
  • each of the bucket supports includes first and second segments that are arranged in a suitably-chosen shape, such as a generally V-shape, a generally T-shape, or a generally Y-shape, for example, with the first and second segments respectively including the first and second trunnions.
  • Each of the first and second trunnions may be oriented at an acute angle relative to an adjacent one of the first or second straps.
  • the first and second straps in one embodiment supporting four buckets, are oriented substantially orthogonal to one another.
  • the rotor may include a rotor hub that is coupled to the rotor core and which is configured for engagement by a centrifuge spindle.
  • the rotor hub is coupled to the rotor core at locations circumferentially spaced from the first and second straps.
  • a centrifuge rotor in another embodiment, has a rotor core that defines an axis of rotation of the rotor, and a plurality of bucket supports each arranged about the axis of rotation. Each bucket support has first and second trunnions, with each trunnion respectively configured to support a bucket.
  • the rotor includes first and second straps oriented generally orthogonal to one another. The first strap extends around a first pair of diametrically-opposed ones of the bucket supports for restricting outward movement of the first pair of bucket supports relative to the axis of rotation. The second strap extends around a second pair of diametrically-opposed ones of the bucket supports for restricting outward movement of the second pair of bucket supports relative to the rotor core. The first and second straps intersect the axis of rotation.
  • a method for making a centrifuge rotor.
  • the method includes arranging a plurality of bucket supports around a rotor core, with the rotor core including an axis of rotation.
  • the method includes coupling a first strap to a first pair of diametrically-opposed ones of the bucket supports to restrict outward movement of the first pair of diametrically- opposed ones of the bucket supports relative to the rotor core.
  • the first strap intersects the axis of rotation of the rotor.
  • the method includes coupling a second strap to a second pair of diametrically-opposed ones of the bucket supports, and arranging the first and second straps such that they intersect one another at the location of intersection of the first strap and the axis of rotation.
  • FIG. 1 is a perspective view of a centrifuge rotor in accordance with one embodiment of the present invention.
  • FIG. 2 is another perspective view of the rotor of FIG. 1 supporting a plurality of open buckets.
  • FIG. 3 is a cross-sectional view taken generally along line 3-3 of FIG. 1 .
  • FIG. 4 is a partially disassembled view of the rotor of FIGS. 1 -3. DETAILED DESCRIPTION
  • FIGS. 1 -4 illustrate an exemplary centrifuge rotor 10 in accordance with one embodiment of the present invention.
  • the rotor 1 0 supports a plurality of swing buckets 12, each configured to hold sample tubes and/or similar laboratory-type containers 13 for centrifugal rotation thereof about a central axis of rotation 14 defined by a rotor core 16 of the rotor 10.
  • Each of the buckets 12 includes a selectively closable lid 12a and a pair of latches 1 2b configured to lock the lid 12a in place during centrifugation.
  • An exemplary bucket 12 suitable for use with rotor 10 is disclosed in U.S. Patent Application No. 12/429,569 entitled SWING BUCKET FOR USE WITH A CENTRIFUGE ROTOR, commonly assigned to the assignee of the present application, and the disclosure of which is hereby expressly incorporated herein by reference in its entirety.
  • the rotor 1 0 includes a plurality of bucket supports 20a, 20b that are arranged for rotation about the axis 14. While the figures illustrate the exemplary bucket supports 20a, 20b being generally V-shaped, it is contemplated that they may alternatively be shaped differently, such as being generally T-shaped or generally Y- shaped, for example, or have any other shapes.
  • the particular arrangement of the bucket supports 20a, 20b is such that each of the bucket supports 20a, 20b supports two of the buckets 1 2. More specifically, each bucket support 20a, 20b includes a pair of segments 22, 24, each having at a longitudinal end thereof a trunnion or pin 22a, 24a (FIG. 4), that is configured to support one of the buckets 12.
  • each of the trunnions 22a, 24a engages a bushing 30 extending from a side wall of a bucket 12 to thereby support the bucket 1 2 in the illustrated generally vertical orientation of the bucket 12, as well as in the generally horizontal orientation (not shown) of the bucket 1 2 during centrifugation.
  • the bucket supports 20a, 20b define an outer perimeter of the rotor 10, as illustrated in FIGS. 1 -2.
  • the rotor 10 unlike conventional swing bucket centrifuge rotors, does not have an outer wall or rim or a solid body defining such outer wall or rim.
  • rotor 1 0 may have an optional circumferentially extending outer shell or shield (not shown), for example, to reduce aerodynamic drag and windage noise, which may be desirable, for example, to facilitate greater temperature control and reduce the required power to drive the rotor 10.
  • the rotor core 1 6 includes a first pair of elongate members 31 extending from a central portion 16a of the rotor core 16 and spanning between a first pair of diametrically opposed bucket supports 20a, and a second pair of elongate members 33 extending from the central portion 16a and spanning between a second pair of diametrically opposed bucket supports 20b.
  • Each of the bucket supports 20a, 20b accordingly, is located at the longitudinal end of each of the elongate members 31 , 33.
  • the central portion 16a of the rotor core 1 6 includes a plurality of holes 34 that, as explained in further detail below, facilitate coupling of the rotor 10 with a centrifuge spindle (not shown) for high-speed rotation of rotor 10.
  • the rotor 10 maintains the required structural integrity during centrifugation. Such structural integrity is facilitated, in this exemplary embodiment, by a pair of reinforcing straps oriented substantially orthogonal to one another, and which restrict outward movement of the bucket supports 20a, 20b relative to the rotor core 16 and, particularly, relative to the central portion 1 6a of rotor core 16. More specifically, the rotor 10 includes a first strap 36 and a second strap 38.
  • the first strap 36 extends around and is operatively coupled to each of the first pair of diametrically-opposed bucket supports 20a, while the second strap 38 extends around and is operatively coupled to each of the second pair of diametrically- opposed bucket supports 20b.
  • the orientation of the straps 36, 38 is such that each of the segments 22, 24 and, particularly, each of the trunnions 22a, 24a of each bucket support 20a, 20b, extends in a direction defining an acute angle relative to the respective strap 36, 38 to which the respective bucket support 20a, 20b is coupled.
  • the acute angle illustrated in the figures is merely exemplary rather than limiting, insofar as other acute angles are contemplated. More specifically, the acute angle in this embodiment is about 45 degrees, by virtue of the specific arrangement of the four bucket supports 20a, 20b and the four buckets 12 supported by the bucket supports 20a, 20b.
  • the present disclosure contemplates other embodiments having buckets 12 (and buckets supports 20a, 20b) in other numbers, such as two, six or eight, for example.
  • the respective acute angles defined by the orientation between the trunnions 22a, 24a and an adjacent strap 36, 38 are larger than about 45 degrees.
  • each of the straps 36, 38 is made of a light, yet strong material, such as fibrous material, a non-fibrous material, a composite material, or others, for example.
  • the straps 36, 38 are made of high-strength carbon fiber in a thermosetting resin, although this is merely exemplary rather than intended to be limiting. Suitable alternatives include other coated or uncoated high tensile-strength fibers.
  • such alternatives may include a carbon fiber in a thermoplastic resin, or an uncoated carbon fiber.
  • the straps 36, 38 may be formed, for example, by winding thermoplastic or thermosetting resin-coated filaments or strands of carbon fiber around the respective pairs of diametrically opposed bucket supports 20a, 20b and then applying pressure and heat to mold the strands into a unitary structure.
  • the resin may be allowed to cure for a predetermined length of time, so as to make it integral with other portions of the rotor 10.
  • Each of the straps 36, 38 is wrapped around respective pairs of the bucket supports 20a, 20b, as illustrated in the figures, to thereby resist outward movement of the bucket supports 20a, 20b away from rotor core 1 6 during high-speed rotation.
  • Each of the straps 36, 38 is respectively positioned over and supported by the elongate members 31 , 33 of the rotor core 16.
  • each of the bucket supports 20a, 20b includes a groove 40 (FIG. 4) that is suitably shaped and sized to receive a portion of one of the straps 36, 38 therein, to thereby secure the respective strap 36, 38 against movement relative to the respective bucket support 20a, 20b and relative to the elongate members 31 , 33 during use.
  • the grooves 40 also provide a path to guide the straps 36, 38 during manufacturing of the rotor 10.
  • the first and second straps 36, 38 are arranged in the rotor 10 so as to respectively define first and second loops, with the first loop being smaller than the second loop.
  • the first strap 36 defines a first loop that is smaller, in the vertical direction of the figures, than the second loop corresponding to the second strap 38.
  • the shape and dimensions of the first loop are also determined by the shape and dimensions of the first elongate member 31
  • the shape and dimensions of the second loop are determined by the shape and dimensions of the second elongate member 33 of rotor core 16.
  • This dimensional relationship of the straps 36, 38 facilitates their placement at the central portion 16a of rotor core 16.
  • the straps 36, 38 intersect one another at the location of central portion 16a that is also intersected by the axis of rotation 14.
  • the second strap 38 surrounds the first strap 36 such that the first strap 36 is completely within the second loop defined by the second strap 38.
  • the straps 36, 38 may be formed from different sizes of tow or unidirectional tape, made for example and without limitation, of carbon fiber, Kevlar, or glass, such that the respective strands of the first and second straps 36, 38 are intertwined (i.e., interlaced) with one another.
  • Such alternative arrangement would thus result in first and second loops that are not necessarily different in size relative to one another.
  • the straps 36, 38 may be made of other fibrous or non-fibrous high tensile-strength materials, so long as they provide the required structural integrity to the rotor 10.
  • Each of the straps 36, 38 includes a respective upper surface 36a, 38a.
  • the upper surface 38a of the second strap 38 lies generally in a slightly curved plane in the span between the two bucket supports 20b to which the second strap 38 is coupled.
  • the upper surface 36a of the first strap 36 also lies in a slightly curved plane in the span between the two bucket supports 20a to which the first strap 36 is coupled, but to a lesser extent than the upper surface 38a of strap 38.
  • the second strap 38 is embedded within each of a pair of the grooves 40 of bucket supports 20b such that the plane in which the upper surface 38a lies also intersects the bucket supports 20b, specifically an upper surface 20c thereof.
  • the second strap 38 in this embodiment is slightly raised in the portion of strap 38 proximate the central portion 16a of rotor core 16, to thereby accommodate the first strap 36 at the central portion 16a.
  • These dimensional relationships define a rotor 10 that is simple to manufacture and is less bulky than conventional rotors.
  • the slight raise of the second strap 38 is facilitated by a correspondingly greater height of the elongate member 33 relative to other portions thereof proximate the central portion 16a.
  • the rotor 10 includes a rotor hub 50 that facilitates engagement of rotor 10 by a spindle (not shown) for centrifugal rotation of the rotor 10.
  • the rotor hub 50 is coupled to the central portion 16a of rotor core 16 so as not to interfere with the portions of the straps 36, 38 therein. More specifically, the rotor hub 50 is coupled to the central portion 16a through two or more drive pins 52 (there are four such drive pins 52 in this embodiment) extending between adjacent portions of the straps 36, 38 and therefore spaced circumferentially from each of the straps 36, 38.
  • the drive pins 52 extend vertically and are spaced circumferentially from one another between adjacent straps 36, 38 and are received through the holes 34 in central portion 16a of rotor core 16.
  • the drive pins 52 are also supported within corresponding bores at an underside of a coupler 59 that secures the rotor 10 to the driving centrifuge spindle (not shown).
  • the outer surfaces of the drive pins 52 are tangent to and in contact with respective side edges 36e, 38e of the straps 36, 38.
  • the rotor 10 is operated by mounting the rotor hub 50 over a suitably chosen centrifuge spindle (not shown). More specifically, the spindle is received within a hub aperture 60 at the bottom of rotor hub 50. When the spindle is actuated, rotation of the spindle causes the drive pins 52 to transfer the driving torque to the rotor core 16, which in turn rotates the rotor 1 0, including the buckets 12.

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PCT/US2010/059231 2009-12-07 2010-12-07 Fiber-reinforced swing bucket centrifuge rotor and related methods Ceased WO2011071880A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2012543197A JP5728491B2 (ja) 2009-12-07 2010-12-07 繊維強化されたスイングバケットを備えた遠心ロータ、及び当該遠心ロータを製造するための方法
CN201080055353.1A CN102712001B (zh) 2009-12-07 2010-12-07 纤维加强摇摆桶式离心机转子及相关方法
DE112010004713.8T DE112010004713B4 (de) 2009-12-07 2010-12-07 Faserverstärkter Zentrifugenrotor für Schwingbecher und Verfahren zur Herstellung eines derartigen Zentrifugenrotors
GB1209783.8A GB2488476B (en) 2009-12-07 2010-12-07 Fiber-reinforced swing bucket centrifuge rotor and related methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/631,999 US8328708B2 (en) 2009-12-07 2009-12-07 Fiber-reinforced swing bucket centrifuge rotor and related methods
US12/631,999 2009-12-07

Publications (1)

Publication Number Publication Date
WO2011071880A1 true WO2011071880A1 (en) 2011-06-16

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PCT/US2010/059231 Ceased WO2011071880A1 (en) 2009-12-07 2010-12-07 Fiber-reinforced swing bucket centrifuge rotor and related methods

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US (1) US8328708B2 (enExample)
JP (1) JP5728491B2 (enExample)
CN (1) CN102712001B (enExample)
DE (1) DE112010004713B4 (enExample)
GB (1) GB2488476B (enExample)
WO (1) WO2011071880A1 (enExample)

Families Citing this family (7)

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Publication number Priority date Publication date Assignee Title
US8147393B2 (en) * 2009-01-19 2012-04-03 Fiberlite Centrifuge, Llc Composite centrifuge rotor
US8147392B2 (en) * 2009-02-24 2012-04-03 Fiberlite Centrifuge, Llc Fixed angle centrifuge rotor with helically wound reinforcement
US8323170B2 (en) * 2009-04-24 2012-12-04 Fiberlite Centrifuge, Llc Swing bucket centrifuge rotor including a reinforcement layer
US8211002B2 (en) * 2009-04-24 2012-07-03 Fiberlite Centrifuge, Llc Reinforced swing bucket for use with a centrifuge rotor
US8328708B2 (en) 2009-12-07 2012-12-11 Fiberlite Centrifuge, Llc Fiber-reinforced swing bucket centrifuge rotor and related methods
JP6572009B2 (ja) * 2015-06-19 2019-09-04 株式会社久保田製作所 遠心分離機のスイング型ロータ用バケット
EP3759208A1 (en) 2018-03-02 2021-01-06 Thermo Electron LED GmbH Single-use centrifuge containers for separating biological suspensions and methods of use

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US8328708B2 (en) 2012-12-11
US20110136647A1 (en) 2011-06-09
CN102712001B (zh) 2014-04-02
DE112010004713B4 (de) 2018-02-08
GB2488476B (en) 2014-06-18
JP2013512777A (ja) 2013-04-18
GB2488476A (en) 2012-08-29
GB201209783D0 (en) 2012-07-18
DE112010004713T5 (de) 2013-05-02
JP5728491B2 (ja) 2015-06-03
CN102712001A (zh) 2012-10-03

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