WO2011056005A3 - 안티센스 가닥에 의한 오프-타겟 효과를 최소화한 신규한 siRNA 구조 및 그 용도 - Google Patents
안티센스 가닥에 의한 오프-타겟 효과를 최소화한 신규한 siRNA 구조 및 그 용도 Download PDFInfo
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- WO2011056005A3 WO2011056005A3 PCT/KR2010/007771 KR2010007771W WO2011056005A3 WO 2011056005 A3 WO2011056005 A3 WO 2011056005A3 KR 2010007771 W KR2010007771 W KR 2010007771W WO 2011056005 A3 WO2011056005 A3 WO 2011056005A3
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- sirna
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- sirna molecule
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- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/14—Type of nucleic acid interfering N.A.
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands
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- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
- C12N2320/53—Methods for regulating/modulating their activity reducing unwanted side-effects
Abstract
본 발명은 신규한 siRNA 구조 및 그 용도에 관한 것으로, 보다 상세하게는 안티센스 및 센스로 구성되는 이중가닥의 siRNA 분자로서 상기 siRNA의 안티센스 상에, 특히 5' 말단의 2번째 위치에, 단일 뉴클레오티드의 도입에 의한 단일 뉴클레오티드 벌지(bulge)를 적어도 하나 이상 가지는 것을 특징으로 하는 siRNA 분자 및 이를 이용한 목적 유전자의 발현 억제 방법에 관한 것이다. 본 발명에 따른 siRNA 분자는 우수한 목적 유전자 발현 억제 효율을 보이면서도, 안티센스 가닥에 의한 오프-타겟 효과를 최소화한 타겟 선별성이 향상된 새로운 구조의 siRNA 분자를 제공함으로써, 종래의 siRNA 분자들을 대체하여 유전자 치료 등 siRNA를 기반으로 하는 유전자 발현 억제 기법에 널리 사용될 수 있다.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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US13/505,965 US9260470B2 (en) | 2009-11-04 | 2010-11-04 | SiRNA structure for minimizing off-target effects caused by antisense strands, and use thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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KR20090105808 | 2009-11-04 | ||
KR10-2009-0105808 | 2009-11-04 |
Publications (2)
Publication Number | Publication Date |
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WO2011056005A2 WO2011056005A2 (ko) | 2011-05-12 |
WO2011056005A3 true WO2011056005A3 (ko) | 2011-10-27 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/KR2010/007771 WO2011056005A2 (ko) | 2009-11-04 | 2010-11-04 | 안티센스 가닥에 의한 오프-타겟 효과를 최소화한 신규한 siRNA 구조 및 그 용도 |
Country Status (3)
Country | Link |
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US (1) | US9260470B2 (ko) |
KR (1) | KR101237036B1 (ko) |
WO (1) | WO2011056005A2 (ko) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2543372A1 (en) * | 2011-07-08 | 2013-01-09 | Helmholtz-Zentrum für Infektionsforschung GmbH | Medicament for the treatment of liver cancer |
ES2627120T3 (es) * | 2011-07-08 | 2017-07-26 | Helmholtz-Zentrum für Infektionsforschung GmbH | Medicamento para el tratamiento del cáncer de hígado |
CN108148838A (zh) * | 2012-05-22 | 2018-06-12 | 奥利克斯医药有限公司 | 具有细胞内穿透能力的诱导rna干扰的核酸分子及用途 |
EP2865757A1 (en) | 2013-10-22 | 2015-04-29 | Sylentis, S.A.U. | siRNA and their use in methods and compositions for inhibiting the expression of the PDK1 gene. |
EP2865756A1 (en) | 2013-10-22 | 2015-04-29 | Sylentis, S.A.U. | siRNA and their use in methods and compositions for inhibiting the expression of the FLAP gene. |
EP2865758A1 (en) | 2013-10-22 | 2015-04-29 | Sylentis, S.A.U. | siRNA and their use in methods and compositions for inhibiting the expression of the ORAI1 gene |
WO2015093769A1 (ko) * | 2013-12-17 | 2015-06-25 | 성균관대학교산학협력단 | 오프-타겟을 막기 위해 변형된 rna 간섭 유도 핵산 및 그 용도 |
KR101696704B1 (ko) | 2013-12-17 | 2017-01-16 | 주식회사 인코드젠 | 오프-타겟을 막기 위해 변형된 rna 간섭 유도 핵산 및 그 용도 |
MA44908A (fr) | 2015-09-08 | 2018-07-18 | Sylentis Sau | Molécules d'arnsi et leur utilisation dans des procédés et des compositions pour inhiber l'expression du gène nrarp |
WO2018098352A2 (en) | 2016-11-22 | 2018-05-31 | Jun Oishi | Targeting kras induced immune checkpoint expression |
CA3046963A1 (en) | 2016-12-14 | 2018-06-21 | Janssen Biotech, Inc. | Cd8a-binding fibronectin type iii domains |
KR102321426B1 (ko) | 2017-02-21 | 2021-11-05 | 올릭스 주식회사 | 남성형 탈모 표적 유전자의 발현을 억제하는 비대칭 siRNA |
JP7162918B2 (ja) * | 2018-05-31 | 2022-10-31 | コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション | マイクロrnaを抑制する変形核酸及びその用途 |
KR102321425B1 (ko) * | 2019-01-18 | 2021-11-04 | 올릭스 주식회사 | NRL(Neural retina leucine zipper)의 발현을 억제하는 비대칭 siRNA |
CN114786682A (zh) | 2019-10-14 | 2022-07-22 | Aro生物疗法公司 | 结合cd71的纤维粘连蛋白iii型结构域 |
WO2021076574A2 (en) | 2019-10-14 | 2021-04-22 | Aro Biotherapeutics Company | Fn3 domain-sirna conjugates and uses thereof |
KR102259402B1 (ko) * | 2020-06-04 | 2021-06-01 | 주식회사 아임뉴런바이오사이언스 | 개선된 안정성을 갖는 핵산 분자 및 이의 용도 |
WO2022020259A1 (en) * | 2020-07-19 | 2022-01-27 | Centrillion Technologies, Inc. | Methods and devices for detecting and sequencing sars-cov-2 |
EP4279591A1 (en) * | 2021-01-15 | 2023-11-22 | The University of Tokyo | Rna molecule, chimeric na molecule, double-stranded rna molecule, and double-stranded chimeric na molecule |
KR20240031947A (ko) * | 2021-04-14 | 2024-03-08 | 에이알오 바이오테라퓨틱스 컴패니 | Fn3 도메인-시르나 접합체 및 이의 용도 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050020521A1 (en) * | 2002-09-25 | 2005-01-27 | University Of Massachusetts | In vivo gene silencing by chemically modified and stable siRNA |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7750144B2 (en) * | 2003-06-02 | 2010-07-06 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of RNA silencing |
ES2873350T3 (es) * | 2007-08-27 | 2021-11-03 | 1Globe Health Inst Llc | Composiciones de ARN interferente asimétrico y usos de las mismas |
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- 2010-11-04 WO PCT/KR2010/007771 patent/WO2011056005A2/ko active Application Filing
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---|---|---|---|---|
US20050020521A1 (en) * | 2002-09-25 | 2005-01-27 | University Of Massachusetts | In vivo gene silencing by chemically modified and stable siRNA |
Non-Patent Citations (4)
Title |
---|
DOENCH, J. G. ET AL.: "siRNAs can function as miRNAs.", GENES DEV., vol. 17, 15 February 2003 (2003-02-15), pages 438 - 442, XP002982608, DOI: doi:10.1101/gad.1064703 * |
MARTIN, S. E. ET AL.: "Mismatched siRNAs downregulate mRNAs as a function of target site location.", FEBS LETTERS., vol. 580, 5 June 2006 (2006-06-05), pages 3694 - 3698, XP028030691, DOI: doi:10.1016/j.febslet.2006.05.056 * |
OHRT, T. ET AL.: "Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells.", NUCLEIC ACIDS RESEARCH, vol. 36, 8 October 2008 (2008-10-08), pages 6439 - 6449 * |
SAXENA, S. ET AL.: "Small RNAs with Imperfect Match to Endogenous mRNA Repress Translation.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 45, 2 September 2003 (2003-09-02), pages 44312 - 44319 * |
Also Published As
Publication number | Publication date |
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KR20110049733A (ko) | 2011-05-12 |
US20130130377A1 (en) | 2013-05-23 |
US9260470B2 (en) | 2016-02-16 |
KR101237036B1 (ko) | 2013-02-25 |
WO2011056005A2 (ko) | 2011-05-12 |
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