WO2010147372A3 - 말라리아 원충 검출용 프라이머, 탐침 및 이를 이용한 검출방법 - Google Patents

말라리아 원충 검출용 프라이머, 탐침 및 이를 이용한 검출방법 Download PDF

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WO2010147372A3
WO2010147372A3 PCT/KR2010/003846 KR2010003846W WO2010147372A3 WO 2010147372 A3 WO2010147372 A3 WO 2010147372A3 KR 2010003846 W KR2010003846 W KR 2010003846W WO 2010147372 A3 WO2010147372 A3 WO 2010147372A3
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plasmodium
primer
probe
malaria
same
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PCT/KR2010/003846
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WO2010147372A9 (ko
WO2010147372A2 (ko
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구완림
김성열
박해준
박한오
변상진
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(주)바이오니아
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Publication of WO2010147372A3 publication Critical patent/WO2010147372A3/ko
Publication of WO2010147372A9 publication Critical patent/WO2010147372A9/ko

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    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 말라리아 원충 검출용 프라이머, 탐침 및 이를 이용한 검출방법에 관한 것으로, 보다 상세하게는 생물학적 시료 및 환경 시료에 존재하는 말라리아 원충의 유전자를 검출하기 위한 프라이머, 탐침 및 이를 이용한 중합효소 연쇄반응으로 말라리아 원충의 유전자를 검출하는 방법에 관한 것이다. 본 발명에 따르면, 종래의 말라리아 원충 검출방법에 비해 신속하고 정확하게 검출할 수 있을 뿐만 아니라, 시료 내에 열대열 말라리아(Plasmodium falciparum), 삼일열 말라리아(Plasmodium vivax), 사일열 말라리아(Plasmodium malariae), 난형 말라리아(Plasmodium ovale)가 혼재되어 있을지라도 한번에 실시간으로 검출가능하다. 또한 상기 프라이머 및 탐침을 포함하는 중합효소 연쇄반응 혼합물의 건조물은 용액 상태와 동등한 성능으로 보관 기간이 향상되므로 검출 키트에 이용될 수 있다.
PCT/KR2010/003846 2009-06-16 2010-06-15 말라리아 원충 검출용 프라이머, 탐침 및 이를 이용한 검출방법 WO2010147372A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090053608A KR20100135128A (ko) 2009-06-16 2009-06-16 말라리아 원충 검출용 프라이머, 탐침 및 이를 이용한 검출방법
KR10-2009-0053608 2009-06-16

Publications (3)

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WO2010147372A2 WO2010147372A2 (ko) 2010-12-23
WO2010147372A3 true WO2010147372A3 (ko) 2011-07-14
WO2010147372A9 WO2010147372A9 (ko) 2011-09-01

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WO (1) WO2010147372A2 (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104284986B (zh) * 2012-04-25 2016-10-12 中国医学科学院基础医学研究所 用于高通量检测疟原虫的方法、组合物和试剂盒
CN104531840A (zh) * 2014-11-26 2015-04-22 中华人民共和国上海出入境检验检疫局 一种快速灵敏的恶性疟原虫检测方法
CN104673919A (zh) * 2015-02-27 2015-06-03 中国疾病预防控制中心寄生虫病预防控制所 一种用于鉴别人体疟原虫种类的试剂盒及其方法
US20200057063A1 (en) * 2017-04-24 2020-02-20 Kimberly-Clark Worldwide, Inc. Method for detecting presence of a thioether and kit for detecting same
CN109486962A (zh) * 2018-11-16 2019-03-19 合肥欧创基因生物科技有限公司 一种疟原虫核酸分型检测试剂盒及其使用方法
KR102004951B1 (ko) * 2018-12-07 2019-08-01 대한민국 말라리아 감염 진단을 위한 Direct LAMP용 키트 및 방법
WO2021146814A1 (en) * 2020-01-24 2021-07-29 Uti Limited Partnership Ultrasensitive loop mediated isothermal amplification (us-lamp) to detect malaria
CN112458181A (zh) * 2020-11-25 2021-03-09 福州海关技术中心 一种用于检测布赫纳蝗螨的引物探针组及其应用
KR102589646B1 (ko) 2021-07-16 2023-10-16 대한민국(질병관리청장) 말라리아 5종 검출용 프라이머 세트

Citations (2)

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US20020102620A1 (en) * 1999-05-07 2002-08-01 S. Melissa Maret Antibodies and peptides for detection of plasmodium vivax
KR20030032081A (ko) * 2001-10-09 2003-04-26 (주)바이오니아 열대열말라리아와 삼일열말라리아의 동시 검출방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020102620A1 (en) * 1999-05-07 2002-08-01 S. Melissa Maret Antibodies and peptides for detection of plasmodium vivax
KR20030032081A (ko) * 2001-10-09 2003-04-26 (주)바이오니아 열대열말라리아와 삼일열말라리아의 동시 검출방법

Non-Patent Citations (4)

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Title
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F. PERANDIN ET AL.: "Development of a Real-Time PCR Assay for Detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for Routine Clinical Diagnosis.", JOURNAL OF CLINICAL MICROBIOLOGY., vol. 42, no. 3, 2004, pages 1214 - 1219, XP002570515, DOI: doi:10.1128/jcm.42.3.1214-1219.2004 *
MATHIEU ROUGEMONT ET AL.: "Detection of Four Plasmodium Species in Blood from Humans by 18S rRNA Gene Subunit-Based and Species-Specific Real-Time PCR Assays.", JOURNAL OF CLINICAL MICROBIOLOGY., vol. 42, no. 12, 2004, pages 5636 - 5643, XP002492831, DOI: doi:10.1128/JCM.42.12.5636-5643.2004 *
SAMEER ELSAYED ET AL.: "Use of Molecular Beacon Probes for Real-Time PCR Detection of Plasmodium falciparum and Other Plasmodium Species in Peripheral Blood Specimens.", JOURNAL OF CLINICAL MICROBIOLOGY., vol. 44, no. 2, 2006, pages 622 - 624, XP055235785, DOI: doi:10.1128/JCM.44.2.622-624.2006 *

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KR20100135128A (ko) 2010-12-24
WO2010147372A9 (ko) 2011-09-01
WO2010147372A2 (ko) 2010-12-23

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