WO2010094228A1 - 大便标本自动检测仪 - Google Patents
大便标本自动检测仪 Download PDFInfo
- Publication number
- WO2010094228A1 WO2010094228A1 PCT/CN2010/070581 CN2010070581W WO2010094228A1 WO 2010094228 A1 WO2010094228 A1 WO 2010094228A1 CN 2010070581 W CN2010070581 W CN 2010070581W WO 2010094228 A1 WO2010094228 A1 WO 2010094228A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- specimen
- stool
- detector according
- automatic detector
- sample
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00534—Mixing by a special element, e.g. stirrer
Definitions
- the present invention relates to a medical detecting device, and more particularly to an automatic stool detecting instrument.
- the technical problem to be solved by the present invention is to provide a detector capable of automatically performing quantitative dilution and detection on stool specimens, which can be automatically cleaned after detection to avoid contamination.
- a stool specimen automatic detector comprising the following parts: an automatic controller; a dilution device for adding a quantitative diluent to a stool sample; a stirring and mixing device, The diluted stool sample is stirred and mixed; the detecting unit is used for detecting the stool specimen; the aspirating sample and the cleaning device are connected to the detecting unit through a pipeline, and are used for conveying the stool specimen to the detecting unit, and at the end of the detecting After cleaning the detection unit and the connecting pipe.
- the detecting unit includes a physical detecting unit and a chemical detecting unit.
- the physical detecting unit comprises a microscope, a microscope camera mounted on the microscope, and a counting cell on the microscope stage, the counting pool being connected to the sampling and cleaning device.
- the sample suction and cleaning device comprises a sample aspirating needle, a diluent inlet, and a sample aspirating pump connected between the sample aspirating needle and the diluent inlet, the counting pool being connected to the aspirating needle and sucking Like a peristaltic pump.
- an electromagnetic pinch-off valve is disposed at each end of the counting pool.
- the loading pin is fixed on the first lifting frame, and the first lifting frame is driven by the first motor.
- the dilution device includes a sampler connected to the diluent inlet, and the sampler is connected A check valve is connected to the suction needle.
- the applicator is a syringe applicator.
- sampler is connected to the second lifting frame, and the second lifting frame is driven by the second motor.
- the chemical detection unit comprises a chemical detection chamber, and the chemical detection chamber is also connected between the aspiration needle and the aspiration peristaltic pump.
- the chemical detecting unit comprises a chemical detecting chamber, one end of the chemical detecting chamber is connected with the counting pool, the other end is connected with a waste liquid peristaltic pump, and the waste liquid peristaltic pump is connected with a waste liquid needle.
- an electromagnetic pinch-off valve is disposed between the chemical detection chamber and the counting pool.
- a specimen box holder is disposed below the first lifting frame.
- it further includes a video camera opposite the side of the specimen holder.
- a video box identification photoelectric switch is disposed above the video camera.
- the specimen holder is mounted on a linear guide that is driven by a stepper motor.
- the specimen box further includes a specimen box.
- the stirring and mixing device comprises a stirring motor and a rotatable sampling spoon, wherein the sampling spoon is located in the specimen box, and when the first lifting frame is lowered, the sampling needle is inserted into the specimen box, And the stirring motor is coaxially connected with the sampling spoon.
- a display is further connected to the automatic controller.
- the automatic controller of the invention can control the dilution device to automatically quantitatively dilute the stool sample, control the mixing and mixing device to mix the sample, control the sample and the cleaning device to send the sample to the detection unit through the pipeline for detection, and at the end of the detection. After cleaning the detection unit and the connecting pipe.
- the whole process is carried out completely in a relatively closed pipeline, which reduces the contact with the outside, reduces the pollution to the environment and the laboratory, and automatically controls the computer, which improves the work efficiency.
- Fig. 1 is a schematic view showing the composition of functional modules of an automatic detector for stool specimens of the present invention.
- FIG 2 is a schematic view of a pipe connection according to an embodiment of the present invention.
- FIG 3 is a schematic view of a pipe connection according to another embodiment of the present invention.
- Figure 4 is a schematic view of the structure of the present invention (forward direction).
- Figure 5 is a schematic view of the structure of the present invention (left-view direction).
- Figure 6 is a schematic view showing the structure of a specimen box.
- the reference numerals are as follows:
- Electromagnetic pinch-off valve 132 opening
- the automatic detector of the stool specimen of the invention comprises an automatic controller, a dilution device, a stirring and mixing device, a detecting unit, a sample and a cleaning device, and the like; wherein the dilution device is under the control of an automatic controller.
- the quantitative dilution solution can be added to the stool sample; the mixing and mixing device can be stirred and mixed under the control of the automatic controller; the detection unit is used for detecting the stool specimen; the sample suction and cleaning device Connected to the detection unit through the pipeline, under the control of the automatic controller, the stool specimen can be transported to the detection unit, and after the detection is completed The detection unit and the connecting pipe are automatically cleaned for the next inspection.
- the above detecting unit may include a physical detecting unit and a chemical detecting unit.
- the automatic controller can be a computer.
- the collected stool specimen is placed in the specimen box 13, the aspiration needle 16 is inserted into the specimen box 13, and the sampler 11 is connected to the diluent inlet 12 through a pipe, at the sampler 11 and the diluent inlet 12 A three-way pipe electromagnetic pinch-off valve 24 and a first pipe tee 21 are provided, and the suction pin 16 is connected to the three-way pipe electromagnetic pinch-off valve 24 through a second pipe tee 22 and a one-way valve 32.
- the three-way pipe electromagnetic pinch-off valve 24 communicates with the sampler 11 and the diluent inlet 12, the line between the sampler 11 and the sample aspirating needle 16 is also disconnected, and the sampler 11 can be removed from the diluent inlet 12 A certain amount of the diluent is aspirated, and then the three-way pipe electromagnetic pinch-off valve 24 is switched, the line between the sampler 11 and the diluent inlet 12 is disconnected, and the pipette 11 and the sample needle 16 are turned on at the same time.
- the tubing pushes the sampler 11, and the diluent is injected into the specimen box 13 through the check valve 32 and the aspirating needle 16, and the quantitative dilution of the stool specimen is completed.
- the diluent inlet 12, the three-way pipe electromagnetic pinch-off valve 24, the sampler 11, the check valve 32 and the suction needle 16 and the connecting pipe thereof constitute a dilution device.
- One end of the aspirating peristaltic pump 18 is connected to the diluent inlet 12 through the first pipe tee 21, and the other end is connected to the aspirating needle 16 through the pipe and the second pipe tee 22 to constitute a suction sample and a cleaning device.
- the counting chamber 14 of the physical detecting unit and the chemical detecting chamber 15 of the chemical detecting unit are connected in series between the aspirating needle 16 and the aspirating peristaltic pump 18.
- An electromagnetic pinch-off valve 33, 34 is disposed at each end of the counting pool 14.
- the sample liquid in the counting pool can be stabilized as soon as possible, which is convenient for observation and counting under the microscope; and at the same time in the backwash cleaning counting pool
- the electromagnetic pinch-off valves 33, 34 are repeatedly switched, the pressure inside the pipe can be increased to increase the flushing effect.
- the aspirating peristaltic pump 18 is reversed, the diluent enters along the pipeline, and the counting tank 14, the chemical detecting chamber 15 and the aspirating needle 16 are cleaned, and the washed waste liquid flows into the specimen box 13 by the aspirating needle 16. Convenient for centralized processing. Due to the action of the check valve 32, waste liquid can be prevented from flowing into the dilution pipe and causing pollution.
- the chemical detection chamber 15 is not connected in series with the counting cell 14 in the aspirating sample and the cleaning line, and one end of the chemical detecting chamber 15 is electromagnetically pinched off.
- the valve 35 and the third pipe tee 23 are connected to the counter cell 14, the other end is connected to a waste liquid peristaltic pump 19, the waste liquid peristaltic pump 19 is connected to a waste liquid needle 17, and the waste liquid needle 17 is also inserted into the specimen box. 13 in.
- the electromagnetic pinch-off valve 35 is closed, the sample liquid only enters the counting cell 14 for physical detection. If chemical detection is required, when the electromagnetic pinch-off valve 33 is closed and the electromagnetic pinch-off valves 34, 35 are opened, the suction is opened.
- the peristaltic pump 18 and the waste peristaltic pump 19 can re-feed the sample solution into the chemical detection chamber 15 for chemical detection.
- the chemical detection chamber 15 can be added to the test strip with the template, and the specimen is automatically added to the test strip.
- the aspirating peristaltic pump 18 and the waste peristaltic pump 19 are continuously turned on, and the dilution tank is used for the counting tank 14 and the chemical detection chamber. 15 is cleaned, the waste liquid enters the sample box 13 from the waste liquid needle 17, and then the electromagnetic pinch-off valve 33 is opened, and the sample suction needle 16 is also cleaned.
- the forward movement of the aspirating peristaltic pump 18 can complete the function of sucking the specimen into the counting tank 14, which is reversed, and the cleaning of the pipe of the counting tank 14 and the chemical detecting chamber 15 is completed, and the running time and the rotating speed are adjustable.
- the specimen box 13 may have a lid 131.
- the lid 131 is provided with an opening 132 for inserting the aspirating needle 16 and the waste needle 17.
- the lid 131 is further provided with a rotating shaft 133.
- the rotating shaft 133 can be freely rotated with respect to the cover.
- the upper end of the rotating shaft 133 can be connected with the motor, and the lower end is connected with a sampling spoon 134.
- the sampling spoon 134 can be used for collecting the stool sample, and putting it into the specimen box and adding the diluent, the motor When the rotating shaft 133 is rotated, the sampling spoon 134 can also perform the function of stirring and mixing.
- the specimen box 13 is provided with a filter 135.
- the filter 135 divides the space inside the box into an upper chamber and a lower chamber, and the lower chamber is a buffer chamber 136.
- the sample needle 16 can be inserted into the buffer chamber 136 to suck the filtered sample liquid.
- the present invention further includes a first lifting frame 41.
- the suction needle 16, the waste liquid needle 17, and a regular rotary joint 44 are all mounted on the first lifting frame 41, and the regular rotary joint 44 is
- the agitating motor 54 is connected.
- the first lifting frame 41 is driven by the first motor 51, and functions to drive the regular rotary joint 44, the suction needle 16, and the waste liquid needle 17 to be lifted and lowered.
- the first is to insert the suction needle 16 and the waste liquid needle 17 into the specimen box 13 to enter.
- the working position of the design is the second, the regular rotary joint 44 is engaged with the rotating shaft of the specimen box cover, so that the sampling spoon 134 is rotated by the stirring motor 54 to perform stirring and mixing.
- the agitating motor 54 and the sampling scoop 134 constitute a stirring and mixing device.
- the sampler 11 uses a syringe type sampler.
- the needle pin of the applicator 11 is coupled to the second lift frame 42, and the second lift frame 42 drives the needle plug to be pulled out and pushed in, thereby drawing in and out of the diluent.
- the second crane 42 is driven by a second motor 52.
- the second motor 52 is controlled by the photoelectric switch to operate the limit.
- the specimen cassette 13 is placed on the specimen holder 61, and the specimen holder 61 is equivalent to the test tube holder and is located below the first lifting frame 41 to accommodate a plurality of specimen cassettes 13.
- the specimen holder 61 is placed on a linear guide 43, which is driven by a stepping motor 53.
- the linear guide 43 is fixed and can drive the specimen holder 61 to operate. The number of runs and the distance of each operation are strictly required, and the operation is controlled according to the program, and the position of the photoelectric switch is verified.
- a video camera 63 is mounted in the vicinity of the specimen holder 61, opposite to the side of the specimen holder 61, and is connected to an automatic controller (computer), and can be used to observe the working state of the sample dilution and agitation while observing the shape and color of the specimen. .
- Above the video camera 63 is a specimen box identifying the photoelectric switch 62, facing the position of the specimen box cover, for identifying the specimen box.
- the linear guide 43 drives the specimen cassette holder 61 to operate, the specimen cassette recognizes the operation of the photoelectric switch 62, and scans the specimen cassette holder 61 with the presence of the specimen cassette, the number of the specimen cassette, and the position of the specimen cassette on the specimen cassette holder 61.
- the instrument automatically operates based on the scan data provided by the specimen box identification photoelectric switch 62. If the specimen box recognizes that the photoelectric switch 62 does not scan the signal to the specimen box, it is not necessary to perform the next detection operation.
- the display 7 is connected to an automatic controller (computer) for program operation and can display a video camera 63 captured video signals.
- the physical detecting unit includes a microscope 8, a microscope camera 81 mounted on the microscope, and a counting cell 14.
- the counting cell 14 is placed on the microscope stage 82 to facilitate observation and counting with a microscope, and images collected by the microscope camera 81 are also displayed. On the display 7.
- the main power source is first turned on, the specimen box 13 is placed on the specimen box holder 61, the specimen box holder 61 is pushed into the instrument from the specimen box frame window, and a switch is triggered, and the stepping motor 53 is positive. Turn, drive the linear guide 43 to run in the positive direction, and run in the opposite direction immediately after the positive direction reaches the forward end.
- the specimen box recognizes the operation of the photoelectric switch 62, detects the presence or absence of the specimen box 13 on the specimen cassette holder 61, and does not detect the specimen box 13, that is, a notification dialog box appears, prompting to place the specimen box 13, and detecting the specimen box 13 Then proceed to the next step.
- the linear guide 43 stops at a position where the first specimen cassette on the specimen cassette 61 is aligned with the aspirating needle 16, the waste needle 17, and the regular rotary joint 44 on the upper first lifting frame 41.
- the video camera 63 is opened to observe the running condition of the instrument, such as whether the regular rotary joint 44 is aligned with the interface on the specimen cover.
- the first motor 51 rotates forward, and the first lifting frame 41 is driven downward, and the stirring motor 54 rotates slowly.
- the first lifting frame 41 drives the sampling needle 16, the waste liquid needle 17, and the regular rotary joint 44 to insert the specimen box 13 Design location.
- the aspirating needle 16 passes through the filter of the specimen box 13 and enters the buffer chamber.
- the regular swivel 44 engages the specimen spool.
- the second motor 52 rotates forward, drives the second lifting frame 42 down, pulls out the needle plug of the syringe type injector 11, and the diluent is sucked. This process is a process in which the sampler 11 draws in the diluent.
- the rear three-way pipe electromagnetic pinch-off valve 24 switches the internal passage, the second motor 52 reverses, the second lift frame 42 ascends, the diluent passes through the check valve 32, the second pipe tee 22, and the suction needle 16
- the sample box 13 is added to complete the filling of the diluent in the pipe. This process is the process of adding a diluent to the specimen box.
- the stirring motor 54 drives the regular rotary joint 44 to rotate in the forward and reverse directions according to the design speed, and drives the sampling spoon in the test tube to rotate, thereby completing the designed rotation time, thereby realizing the mixing of the specimens in the specimen box 13.
- the aspirating peristaltic pump 18 is rotated forward, and the diluted and mixed specimen is injected into the counting pool 14 through the aspirating needle 16, the second conduit tee 22, and the third conduit tee 23, and the inhaled specimen liquid Stop at the rear of the electromagnetic pinch-off valve 34.
- the electromagnetic pinch-off valve 33 and the electromagnetic pinch-off valve 34 at both ends of the counting cell 14 are closed, and the silicone tube is clamped, so that the sample in the counting cell 14 is quickly stopped to facilitate the microscope to detect it.
- the connection line between the counting cell 14 and the aspirating peristaltic pump 18 is sufficiently long that the sample is not drawn into the diluent by the aspirating peristaltic pump 18. This process is to inject the diluted and mixed sample solution into the counting cell 14 for detection.
- the stirring motor 54 is stopped.
- the software displays the prompt for the microscopic examination.
- the microscope in the instrument starts to work.
- the microscope camera sends the microscopic image to the monitor of the computer, observing a field of view
- the operator can press the software button to operate, click the "manual injection” button.
- the aspirating peristaltic pump 18 is rotated forward, the electromagnetic pinch-off valve 33 and the electromagnetic pinch-off valve 34 are opened, and the sample liquid is sucked in a small amount.
- the aspirating peristaltic pump 18 stops operating, and the electromagnetic pinch-off valve 33
- the electromagnetic pinch-off valve 34 is closed again, the pipe is clamped, and the operator performs the microscopic inspection result entry.
- the above process can be performed up to four times, and the operator can control the movement of the microscope stage to achieve the purpose of switching the field of view.
- the above is the microscopic examination process of the sample solution.
- the operator enters the cleaning procedure after pressing the "Print" button.
- the chemical detection pipeline is cleaned, the aspirating peristaltic pump 18 is reversed, and the dilution liquid is cleaned through the first pipeline tee 21, the aspirating peristaltic pump 18, the counting pool 14, the third pipeline tee 23, and the chemical detection chamber 15, and at the same time
- the liquid peristaltic pump 19 is rotated forward, and the waste liquid is discharged into the specimen box 13 through the chemical detection chamber 15, the waste liquid peristaltic pump 19, and the waste liquid needle 17.
- the waste peristaltic pump 19 stops working, the aspirating peristaltic pump 18 rotates forward, and the diluent in the chemical detection chamber 15 and the third conduit tee 23 is drained, and then the electromagnetic pinch-off valve 35 is closed, and the pipeline is closed.
- the suction peristaltic pump 18 is reversed, and the diluent is discharged through the pipeline first pipe tee 21, the suction peristaltic pump 18, the counting pool 14, the third pipe tee 23, the second pipe tee 22, and the suction needle 16
- the sample cell 14 is cleaned while being inside the specimen box 13.
- the instrument will automatically display an alarm dialog box.
- the electromagnetic pinch-off valves 33 and 34 stop working for one second every second, and repeatedly open and close. This method is used to increase the pressure in the cleaning pipe and increase the cleaning effect.
- the above process is the detection of a specimen box and the cleaning process of the instrument.
- the first lifting frame 41 is operated upward.
- the syringe-type sampler 11 sucks in the diluent again, and the linear guide 43 is positioned to the position of the next specimen box according to the signal input by the specimen box identifying the photoelectric switch 62, A specimen performs the same operation.
- the instrument runs the specimen cassette holder 61 to the design position, enters the standby state, and prints a test report.
- the "print" button can be directly pressed, and the instrument enters the cleaning procedure for the counting pool 14, and the subsequent operations are unchanged.
- the operation, operation time and running direction of the motor are controlled by software, and the circuit principle used is known.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10743409.4A EP2400305B1 (en) | 2009-02-19 | 2010-02-09 | Automatic detector for stool specimen |
JP2011550407A JP5384671B2 (ja) | 2009-02-19 | 2010-02-09 | 大便標本自動検査器 |
US13/202,398 US8765057B2 (en) | 2009-02-19 | 2010-02-09 | Automatic detection instrument for stool specimen |
RU2011137987/15A RU2526197C2 (ru) | 2009-02-19 | 2010-02-09 | Автоматический анализатор образцов кала |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200910046365.7 | 2009-02-19 | ||
CN2009100463657A CN101487845B (zh) | 2009-02-19 | 2009-02-19 | 大便标本自动检测仪 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2010094228A1 true WO2010094228A1 (zh) | 2010-08-26 |
Family
ID=40890817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2010/070581 WO2010094228A1 (zh) | 2009-02-19 | 2010-02-09 | 大便标本自动检测仪 |
Country Status (7)
Country | Link |
---|---|
US (1) | US8765057B2 (zh) |
EP (1) | EP2400305B1 (zh) |
JP (1) | JP5384671B2 (zh) |
CN (1) | CN101487845B (zh) |
RU (1) | RU2526197C2 (zh) |
TR (1) | TR201903771T4 (zh) |
WO (1) | WO2010094228A1 (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8765057B2 (en) | 2009-02-19 | 2014-07-01 | Jihong Biotech (Shanghai) Co., Ltd. | Automatic detection instrument for stool specimen |
CN104166005A (zh) * | 2014-08-20 | 2014-11-26 | 湖南欧杰生物科技发展有限公司 | 一种全自动排泌物检测仪 |
CN107014641A (zh) * | 2017-04-27 | 2017-08-04 | 嘉兴鸿利机械有限公司 | 自动取样装置 |
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Also Published As
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CN101487845A (zh) | 2009-07-22 |
TR201903771T4 (tr) | 2019-04-22 |
CN101487845B (zh) | 2013-01-02 |
EP2400305A1 (en) | 2011-12-28 |
US20120051974A1 (en) | 2012-03-01 |
RU2526197C2 (ru) | 2014-08-20 |
JP2012518175A (ja) | 2012-08-09 |
US8765057B2 (en) | 2014-07-01 |
EP2400305A4 (en) | 2017-09-06 |
RU2011137987A (ru) | 2013-03-27 |
JP5384671B2 (ja) | 2014-01-08 |
EP2400305B1 (en) | 2019-01-09 |
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