WO2010057858A1 - Dispositif d’analyse automatique doté d’un carrousel rotatif destiné à différents récipients pour liquides - Google Patents

Dispositif d’analyse automatique doté d’un carrousel rotatif destiné à différents récipients pour liquides Download PDF

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Publication number
WO2010057858A1
WO2010057858A1 PCT/EP2009/065249 EP2009065249W WO2010057858A1 WO 2010057858 A1 WO2010057858 A1 WO 2010057858A1 EP 2009065249 W EP2009065249 W EP 2009065249W WO 2010057858 A1 WO2010057858 A1 WO 2010057858A1
Authority
WO
WIPO (PCT)
Prior art keywords
carousel
reagent
sample
reaction vessel
compartments
Prior art date
Application number
PCT/EP2009/065249
Other languages
German (de)
English (en)
Inventor
Roland Schenk
Original Assignee
Diasys Technologies S.A.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diasys Technologies S.A.R.L. filed Critical Diasys Technologies S.A.R.L.
Priority to EP09764215.1A priority Critical patent/EP2346610B1/fr
Priority to CN200980146763.4A priority patent/CN102215971B/zh
Priority to ES09764215.1T priority patent/ES2687999T3/es
Publication of WO2010057858A1 publication Critical patent/WO2010057858A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0439—Rotary sample carriers, i.e. carousels
    • G01N2035/0453—Multiple carousels working in parallel
    • G01N2035/0455—Coaxial carousels

Definitions

  • the present invention relates to a device for the automated analysis of liquids with a carousel for liquid containers, the carousel comprising a rotatable about a central axis of rotation reagent and / or sample carousel, the reagent / sample carousel reagent and / or sample container compartments for receiving Reagent and / or sample containers.
  • the present invention relates to a corresponding analysis carousel for such a device.
  • analyzers In the context of increasing automation in the field of medical and veterinary diagnostics, i.a. Devices for the automated analysis of liquids, so-called analyzers, developed, which can take a reagent required for the performance of an analysis from a reagent container and combine with a sample for the purpose of carrying out the analysis in a reaction vessel.
  • the analyzers often have a carousel in which either receiving areas for reagent containers or receiving areas for sample containers are provided.
  • special analyzer carousels both receiving areas for reagent containers and receiving areas for sample containers are provided.
  • Such carousels are usually driven by a drive device provided in the analyzer for performing a rotary movement of the carousel.
  • an automatic pipetting device generally comprises a pipetting arm, on which a pipetting needle is arranged, which is connected to a pumping unit, with which a liquid can be drawn up into the pipetting needle and ejected again from the pipetting needle.
  • a pipetting arm is generally designed in such a way that the pipetting needle can be moved over a working area with the pipetting arm, in which working area the reagent containers, sample containers and / or reaction vessels (eg cuvettes) are stationarily arranged or by e.g. B. a carousel be provided temporarily.
  • the pipette needle is usually immersed in a rinsing liquid, with a certain amount of rinsing liquid in the pipette.
  • Pettiemadel is raised. Subsequently, the pipette needle is pulled out of the rinsing liquid and emptied through a waste container or a drain and drained or stripped.
  • analyzers also include a measuring device for determining a physical or chemical quantity of a reaction mixture prepared in a reaction vessel.
  • the reaction vessel may, for. Example, be a cuvette, which is arranged in the beam path of a arranged in the analyzer photometer or can be introduced.
  • analyzers usually include at least one control unit for controlling the movements of the pipetting arm, the pumping unit, the pipetting needle, the lifting column and / or the carousel, and a data processing device for setting up and executing an analysis program and for processing and outputting a measured physical or chemical Size.
  • samples and many reagents must be kept at a certain temperature to keep the substances in them stable.
  • samples and reagents are kept refrigerated and are set in the analyzer just prior to running a run.
  • reaction vessels are arranged should be designed as space-saving.
  • the aim is to mix one or more samples with one or more reagents in reaction vessels in as little space as possible and to analyze the resulting reaction mixtures.
  • liquid container carousel of this device is characterized in that it additionally has a concentrically arranged around the axis of rotation reaction vessel carousel with reaction vessel compartments for receiving reaction vessels, wherein a heat-insulating layer is provided between the reagent and / or sample carousel and the reaction vessel carousel.
  • the compartments for the reagent or sample containers and / or the reaction vessel compartments are arranged radially in the liquid container carousel.
  • the compartments for the reagent or sample containers are arranged in a central area and the reaction vessel compartments outside this area.
  • the arrangement can also be done the other way round.
  • the heat-insulating layer is expediently made of a material with poor heat conductivity properties.
  • the thermal conductivity ⁇ of the material is at most 0.5 W / (m ⁇ K).
  • the thermal conductivity ⁇ of the material is at most 0.1 W / (m ⁇ K). More preferably, the thermal conductivity ⁇ of the material is at most 0.05 W / (m ⁇ K).
  • the heat-insulating layer has a thickness of at least 1 mm.
  • the thickness of the heat-insulating layer is in the range of 1 mm to 10 mm. More preferably, the thickness of the heat-insulating layer is in the range of 1 mm to 5 mm. More preferably, the thickness of the heat-insulating layer is in the range of 1 mm to 3 mm.
  • the heat-insulating layer provided between the carousels may consist of a heat-insulating solid, a heat-insulating liquid cushion or a heat-insulating gas cushion. Particularly preferably, the heat-insulating layer provided between the carousels consists of a heat-insulating solid.
  • the advantage of the present invention is that extremely space-saving a variety of reagent containers, sample containers and reaction vessels can be accommodated in a remplisstechniks employerkarussell and can be rotated in the working range of Pipettierarms. Because the reagents and samples are usually kept at a different temperature - -
  • this space-saving arrangement is only possible in that between the reagent and / or sample carousel and the reaction vessel carousel, a heat-insulating layer is provided.
  • the compartments for accommodating the reaction vessels are arranged on a different plane of the liquid container carousel than the compartments for receiving the reagent or sample containers.
  • the compartments for the reagent or sample containers can be arranged in the liquid container carousel in a central area and the reaction vessel compartments can be arranged outside this area and vice versa. In any event, however, the areas where the reagent / sample compartments are located are not located immediately above or below the area where the reaction vessel compartments are located. This ensures that the pipetting needle can access all liquid containers in the liquid container carousel.
  • the reagent / sample carousel plane is preferably located in a separation device provided by an analyzer in the space in which the reaction vessel carousel is located Area.
  • This separation can be carried out on the different levels in the embodiment in which the reagent or sample carousel and reaction vessel carousel are arranged, for example through a separating tray which has a recess corresponding to the circumference of the reaction vessel carousel and after insertion of the liquid container carousel into the analyzer is disposed at the level which is between the plane on which the reagent / sample carousel is arranged, and the plane on which the reaction vessel carousel is arranged.
  • the reaction vessel carousel is located below the reagent / sample carousel level.
  • the reagent / sample carousel and the liquid container carousel are spaced apart so that there is substantially no contact between the carousels to prevent heat transfer from one carousel to the other carousel.
  • the cushion of ambient air located between the carousels forms the heat-insulating layer.
  • punctiform or strip-shaped contact surfaces may be provided between the carousels of this embodiment, preferably - -
  • between the carousels of this embodiment additionally provided a heat-insulating layer of a heat-insulating solid or a heat-insulating liquid cushion.
  • the heat-insulating layer is in the form of an insulating disk, which is arranged around the rotation axis concentric with the reagent / sample carousel and the reaction vessel carousel.
  • an isolation disk may be mounted on the bottom of the reagent / sample carousel or on top of the reaction vessel carousel in the area where the reagent / sample carousel overlaps with the reaction vessel carousel.
  • the isolation disk is a physically separate isolation disk that can be located between the reagent / sample carousel and the reaction vessel carousel.
  • this isolation disc is detachably connectable to the reagent / sample carousel and / or the reaction vessel carousel.
  • the reagent / sample carousel and the reaction vessel carousel are components of a contiguous carousel for reagent containers and / or sample containers and reaction vessels.
  • the reagent / sample carousel and the reaction vessel carousel are physically separate but releasably interconnectable elements of the carousel.
  • the carousel consists of a total of three physically separate but detachably connectable carousels, namely a reagent carousel, a sample carousel and a reaction vessel carousel.
  • the reagent carousel and the sample carousel are also superimposed on different planes, wherein the region in which the reagent compartments are arranged and the region in which the sample compartments are arranged do not overlap.
  • a heat-insulating layer is preferably provided between reagent carousel and sample carousel, which may also be mounted on the underside or top of one of the carousels or a separate one about the axis of rotation may be concentrically arranged isolation disc.
  • the analyzer carousel according to the invention may have only one carousel for reagents in addition to the reaction vessel carousel. In such an analyzer, the samples are then provided in the analyzer in a different form or can be supplied from the outside. In an alternative embodiment, only one carousel for samples is provided in addition to the reaction vessel carousel. In this case, the reagents are provided in another area of the analyzer or supplied from the outside. In a further alternative embodiment, the analysis - -
  • a carousel contains both reagent compartment and sample fan sections (combined reagent / sample carousel).
  • individual samples or reagents can be introduced into the working area of the pipetting arm from outside via an insertion shaft.
  • Such an insertion slot can also be provided for those embodiments in which areas for reagents and / or samples are already regularly provided in the analyzer.
  • the insertion slot in these cases offers the possibility of retroactive, i. Add samples or reagents to the analyzer during an analysis run.
  • the reagent carousel and / or sample carousel or combined reagent / sample carousel and reaction carousel may be independently rotated about the common axis of rotation.
  • a correspondingly suitable drive device is provided in the analyzer.
  • all partial carousels of the entire carousel are simultaneously rotated about the common axis of rotation via a common drive device.
  • the reaction vessel carousel consists of a good heat-conducting material.
  • the thermal conductivity ⁇ is at least 15 W / (m ⁇ K) (eg, stainless steel). More preferably, the thermal conductivity ⁇ is at least 120 W / (m ⁇ K) (eg, brass). Particularly preferred is a thermal conductivity ⁇ of at least 220 W / (m ⁇ K) (eg aluminum).
  • the reaction vessel carousel preferably consists of a material with a low heat capacity.
  • the specific heat capacity of this material is less than 1.0 J / (g K), more preferably less than 0.9 J / (g K).
  • reaction vessel carousel consists essentially of aluminum. In specific embodiments, the reaction vessel carousel is entirely made of aluminum.
  • the reaction vessel carousel and thus the reaction mixture contained in the reaction vessels arranged in the carousel should be heatable.
  • the carousel is arranged such that the reaction vessel carousel is located in an area that is separated by a separator spatially from the area in which the reagent / sample carousel is located (see also above).
  • the temperature of the reaction vessel carousel can be carried out via an air bath.
  • the temperature control of the reaction vessel carousel takes place via a heating foil or a heating wire (resistance heating) applied to the reaction vessel carousel.
  • the energy supply of this heating foil particularly preferably takes place via a central sliding contact between the reaction vessel carousel and the axis of rotation.
  • a peripheral sliding contact is provided on the underside of the reaction vessel carousel or on the outer circumference of the reaction vessel carousel, via which the energy required for the temperature control of the reaction vessel carousel can be supplied.
  • a temperature sensor is preferably provided on the reaction vessel carousel, which temperature sensor is connected to a corresponding data processing and control unit.
  • the device according to the invention with the described liquid container carousel has one or more elements under a carousel drive device, a pipetting device, a rinsing station, a heat-generating device, a cryogenic device, an optical measuring device for determining a physical or chemical size of the reaction mixture, and an opto-electronic reading device for reading an opto-electronically readable code, which is attached to or on the carousels and / or on the sample and / or reagents.
  • FIG. 1 shows an apparatus for the automated analysis of liquids (analyzer) with a carousel for liquid container according to the present invention, - -
  • Figure 2 is a schematic representation of a carousel for liquid container according to the invention in a side view
  • Figure 3 is a detailed view of a carousel for liquid container according to the present invention, in a sectional view through the axis of rotation of the carousel.
  • FIG. 1 shows an apparatus for the automated analysis of liquids (analyzer), which has a carousel 1 for liquid containers and a pipetting apparatus 2 with a pipetting arm 5.
  • the carousel 1 is rotatably arranged in the region of the bottom of the analyzer and has an overlapping region with the working region of the pipetting arm 5. In this way, the pipetting arm 5 can reach the region with the pipetting needle 27 in which liquid containers are arranged in the carousel 1.
  • the carousel 1 according to the invention is shown in side view.
  • a combined reagent / sample carousel 11/12 can be seen, in which reagent compartments 14 and sample compartments 15 are provided.
  • Reagent containers 100 are set in the reagent compartments 14 and sample containers 101 are set in the sample compartments 15.
  • a reaction vessel carousel 16 can be seen in which reaction vessel compartments 17 are provided, into which reaction vessels 102 are set.
  • the reaction vessel carousel 16 is arranged here below the reagent / sample carousel plane 13.
  • the combined reagent / sample carousel and the reaction vessel carousel have a circular base and are arranged concentrically around the axis of rotation 10. On the axis of rotation 10, the central axis 19 of the carousel 1 is located.
  • a heat-insulating layer 18 is provided between the reagent / sample carousel 1 1/12 and the reaction vessel carousel 16, a heat-insulating layer 18 is provided. It can also be seen on the illustration of FIG. 2 that the region in which the reagent and / or sample compartments 14/15 are arranged in the reagent and / or sample carousel 11/12, and the region in which the reaction vessel carousel 16 is located Reaction vessel fan 17 are arranged, do not overlap. In this particular case, the region in which the reagents and / or sample compartments 14/15 are arranged in the reagent and / or sample carousel 1 1/12 is arranged centrally relative to the axis of rotation 10. In contrast, the region in which the reaction vessel compartments 17 are arranged in the reaction vessel carousel 16 is provided with respect to the rotation axis 10 outside the central region in which the reagent / sample compartments are arranged.
  • FIG. 3 shows a special embodiment of a liquid container carousel 1 inserted into an analyzer.
  • the carousel 1 consisting of the combined reagent - -
  • a base plate 1 is also provided, which can be set in rotational motion by the drive device 105 arranged in the analyzer.
  • a sliding contact 106 is provided on the carousel 1, which can come into contact with a corresponding contact point in the analyzer to receive electrical energy from the analyzer, so as to temper the cuvette carousel.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne un dispositif pour analyser automatiquement des liquides, qui est doté d’un carrousel portant des récipients pour liquides et comprenant un carrousel pour réactifs et/ou échantillons qui peut tourner autour d’un axe de rotation central. Ledit carrousel pour réactifs/échantillons présente, à son niveau, des compartiments pour réactifs et/ou échantillons, destinés à recevoir des récipients pour réactifs et/ou échantillons. L'invention a pour but de fournir un dispositif pour analyser automatiquement des liquides avec lequel on peut prélever des liquides dans une pluralité de récipients pour réactifs et/ou une pluralité de récipients pour échantillons, que l’on peut ensuite mélanger les uns aux autres dans des réacteurs, les réacteurs et les mélanges réactionnels qu’ils contiennent étant amenés à la température souhaitée différemment des réactifs et/ou des échantillons. A cet effet, on propose selon l’invention que le carrousel soit en plus doté d’un carrousel pour réacteurs destiné à recevoir des réacteurs. De plus, on prévoit une couche thermo-isolante entre le carrousel pour réactifs et/ou échantillons et le carrousel pour réacteurs.
PCT/EP2009/065249 2008-11-18 2009-11-16 Dispositif d’analyse automatique doté d’un carrousel rotatif destiné à différents récipients pour liquides WO2010057858A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP09764215.1A EP2346610B1 (fr) 2008-11-18 2009-11-16 Dispositif d analyse automatique doté d un carrousel rotatif destiné à différents récipients pour liquides
CN200980146763.4A CN102215971B (zh) 2008-11-18 2009-11-16 具有用于不同液体容器的可转动转盘的自动分析装置
ES09764215.1T ES2687999T3 (es) 2008-11-18 2009-11-16 Dispositivo para el análisis automatizado con un carrusel giratorio para diferentes recipientes de líquido

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008058062.7 2008-11-18
DE200810058062 DE102008058062A1 (de) 2008-11-18 2008-11-18 Automatisierte Analysevorrichtung mit einem drehbaren Karussell für verschiedene Flüssigkeitsbehälter

Publications (1)

Publication Number Publication Date
WO2010057858A1 true WO2010057858A1 (fr) 2010-05-27

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PCT/EP2009/065249 WO2010057858A1 (fr) 2008-11-18 2009-11-16 Dispositif d’analyse automatique doté d’un carrousel rotatif destiné à différents récipients pour liquides

Country Status (5)

Country Link
EP (1) EP2346610B1 (fr)
CN (1) CN102215971B (fr)
DE (1) DE102008058062A1 (fr)
ES (1) ES2687999T3 (fr)
WO (1) WO2010057858A1 (fr)

Cited By (1)

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CN102248055A (zh) * 2011-05-31 2011-11-23 江阴市创新气门嘴有限公司 多工位气门嘴自动加工冲床

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103048479B (zh) * 2012-12-13 2014-03-26 江阴泽成生物技术有限公司 一种用于全自动化学发光分析仪的试剂样品系统
CN104777321B (zh) * 2015-04-16 2016-07-06 清华大学 化学发光免疫分析装置
CN107238715B (zh) * 2017-06-28 2019-01-18 苏州长光华医生物医学工程有限公司 一种血型仪温育加样机构
CN109254164B (zh) * 2018-10-23 2024-02-27 黄河科技学院 生化免疫分析装置教学实验模型

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US5051238A (en) * 1987-11-20 1991-09-24 Hitachi, Ltd. Automatic analyzing system
US5814277A (en) * 1988-07-25 1998-09-29 Precision Systems, Inc. Automatic multiple-sample multiple-reagent chemical analyzer
US6335166B1 (en) * 1998-05-01 2002-01-01 Gen-Probe Incorporated Automated process for isolating and amplifying a target nucleic acid sequence
EP1767949A1 (fr) * 2005-09-21 2007-03-28 F.Hoffmann-La Roche Ag Ensemble de récipients a réactifs et analyseur comprenant cet ensemble

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US5376313A (en) * 1992-03-27 1994-12-27 Abbott Laboratories Injection molding a plastic assay cuvette having low birefringence
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EP1435001B1 (fr) * 2001-10-09 2006-03-01 Reportex Europe GmbH Dispositif de mesure photometrique
ATE391918T1 (de) * 2004-11-25 2008-04-15 Hoffmann La Roche Vorrichtung zum analysieren von proben
JP3980031B2 (ja) * 2005-02-09 2007-09-19 株式会社日立製作所 自動分析装置
JP4768409B2 (ja) * 2005-11-15 2011-09-07 シスメックス株式会社 試料分析装置、試料分析本体装置、及び試料容器供給装置
DE202006017454U1 (de) * 2006-11-14 2007-05-16 Diasys Technologies S.A.R.L. Reagenzienbehälter und Reagenzienkarussell

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Publication number Priority date Publication date Assignee Title
US5051238A (en) * 1987-11-20 1991-09-24 Hitachi, Ltd. Automatic analyzing system
US5814277A (en) * 1988-07-25 1998-09-29 Precision Systems, Inc. Automatic multiple-sample multiple-reagent chemical analyzer
US6335166B1 (en) * 1998-05-01 2002-01-01 Gen-Probe Incorporated Automated process for isolating and amplifying a target nucleic acid sequence
EP1767949A1 (fr) * 2005-09-21 2007-03-28 F.Hoffmann-La Roche Ag Ensemble de récipients a réactifs et analyseur comprenant cet ensemble

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102248055A (zh) * 2011-05-31 2011-11-23 江阴市创新气门嘴有限公司 多工位气门嘴自动加工冲床

Also Published As

Publication number Publication date
CN102215971A (zh) 2011-10-12
CN102215971B (zh) 2015-07-15
EP2346610B1 (fr) 2018-07-18
EP2346610A1 (fr) 2011-07-27
DE102008058062A1 (de) 2010-05-20
ES2687999T3 (es) 2018-10-30

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