WO2010050073A1 - Système pour stérilisation hypoxique, préservation, transport et manipulation de tissu corporel devant être utilisé dans la médecine régénérative - Google Patents

Système pour stérilisation hypoxique, préservation, transport et manipulation de tissu corporel devant être utilisé dans la médecine régénérative Download PDF

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Publication number
WO2010050073A1
WO2010050073A1 PCT/JP2008/070360 JP2008070360W WO2010050073A1 WO 2010050073 A1 WO2010050073 A1 WO 2010050073A1 JP 2008070360 W JP2008070360 W JP 2008070360W WO 2010050073 A1 WO2010050073 A1 WO 2010050073A1
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WO
WIPO (PCT)
Prior art keywords
sealed
necessary
tissue
storage box
biological tissue
Prior art date
Application number
PCT/JP2008/070360
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English (en)
Japanese (ja)
Inventor
喬 島田
恒憲 大鐘
Original Assignee
デンタルサポート株式会社
特定非営利活動法人 日本パテントリサーチアソシエイツ
株式会社コラソン
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by デンタルサポート株式会社, 特定非営利活動法人 日本パテントリサーチアソシエイツ, 株式会社コラソン filed Critical デンタルサポート株式会社
Priority to PCT/JP2008/070360 priority Critical patent/WO2010050073A1/fr
Publication of WO2010050073A1 publication Critical patent/WO2010050073A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time

Definitions

  • the present invention is intended to propose a novel system capable of storing, transporting, and managing living tissue collected according to various purposes within a desired period while appropriately suppressing the propagation of miscellaneous fungi. is there.
  • cryopreserving cryogenic conditions such as liquid nitrogen
  • it is difficult to implement safely without a research institution with considerable equipment.
  • cryogenicity such as liquid nitrogen is dangerous in itself, and considerable energy and cost are required for maintenance.
  • the present invention has been devised in view of the above-described prior art, and has been devised through research and examination.
  • the technical principle applied to the two-tank differential pressure dust-free cleaning device in a semiconductor wafer manufacturing apparatus is applied.
  • By controlling the amount of oxygen around cells, tissues, and organs to be stored under conditions of vacuum and nitrogen gas or other inert gas atmosphere it effectively prevents the respiration necessary for the propagation of various fungi, and the decay progresses. It is proposed to prevent this, and more specifically is as follows.
  • the obtained biological tissue or the like is held in a predetermined position of a storage box that is equipped with holding means and can be sealed in a vacuum state,
  • the container is sealed and the vacuum state is evacuated to about ⁇ 20 Kpa or less after sealing,
  • the storage box is filled and sealed with inert gas such as nitrogen gas as necessary, Cool the inside of the storage box to a predetermined low temperature state, Continue this sealed low-temperature state by appropriate means, If necessary, record the progress and continuation status of each procedure in the computer device along with the passage of time after acquisition of biological tissue, etc.
  • a system for low-oxygen sterilization / storage / transport / management of biological tissue used for regenerative medicine characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after acquisition of the biological tissue.
  • the extracted deciduous teeth are held in place in a storage box that is equipped with holding means and can be sealed in a vacuum state, In order to prevent the propagation of various germs and maintain and maintain the deciduous teeth state immediately after removal, the container is sealed and the vacuum state in which air is evacuated to at least ⁇ 20 Kpa is provided.
  • the storage box is filled and sealed with inert gas such as nitrogen gas as necessary, Cool the inside of the storage box to a predetermined low temperature state, Continue this sealed low-temperature state by appropriate means, If necessary, record the progress and continuation status of each procedure in the computer device with the passage of time after extracting the deciduous teeth,
  • inert gas such as nitrogen gas
  • the portion containing the dental pulp cells at the tip of the deciduous teeth is protected without being damaged by the pressure contact / fixing means sandwiched from at least two sides. Hold the extracted deciduous teeth in a state that has a shape like a net or a bag and is detachable in a cassette-type device that is pressed and fixed again with tweezers as necessary.
  • the extracted deciduous teeth are automatically and mechanically stored with arm-type vacuum tweezers in a low-oxygen storage room or a cooling storage room that can be controlled at -25 ° C or lower.
  • the living tissue used for regenerative medicine according to any one of the above items (1) to (3), wherein the tissue can be transported without being exposed to air. Transportation and management system.
  • each treatment in a clean state excluding fouling can be performed by washing the tissue surface with physiological saline or the like as long as the acquired biological tissue is not damaged.
  • the obtained biological tissue etc. is held in place in a storage box equipped with holding means and sealed in a vacuum state to propagate various germs
  • a storage box equipped with holding means and sealed in a vacuum state to propagate various germs
  • it is included in the air by sealing the storage box and then evacuating it to at least -20 Kpa (kilopascal) or less. This also substantially reduces and removes the generated oxygen, thereby suppressing the growth of microorganisms such as miscellaneous bacteria that perform oxygen respiration.
  • the storage box It becomes possible to continue to suppress the propagation of germs and the like more effectively while maintaining the inside in a positive pressure state that is easier to manage.
  • an inert gas such as nitrogen gas
  • a pedestal that is a cassette-type device, if necessary, holds the extracted milk teeth in a state where they are pressed and fixed again with tweezers, and is covered with cleaning means such as a cleaning nozzle that covers the entire pedestal.
  • FIG. 1 is a flowchart showing a series of work steps performed by a system apparatus using the present invention.
  • the door 2 of the system device 1 of the present invention is opened, held in a predetermined position of the storage box 3, set and then closed.
  • FIG. 1 An example of the internal configuration of such an apparatus 1 is shown in FIG. That is, the milk teeth can be held, fixed, and moved by the operation arm 7 having the vacuum tweezers 6 attached to the tip while acquiring image information with the CCD camera 5. At this time, a hole may be made at a predetermined location with a drill 8 to facilitate collection of dental pulp tissue.
  • the cleaning nozzle 9 may be covered from above to perform a necessary cleaning process, and the rotor 10 on which the milk teeth are installed may be dried at a low speed of about 100 revolutions per minute. With this low speed rotation, there is no risk of physical damage to the deciduous teeth.
  • a vacuum state is established as a sealed state.
  • oxygen necessary for the propagation of miscellaneous fungi is excluded from the vicinity of the deciduous teeth, and decay during storage and storage is prevented.
  • the vacuum state is a negative pressure state, and the outside air will invade only when the sealed state is slightly impaired, but if it is refilled with a harmless inert gas such as nitrogen gas, the sealed state is temporarily Even if it is damaged, only the internal gas leaks out, and there is no possibility of outside air hitting the stored milk teeth.
  • the deciduous teeth to be stored and transported are fixed and moved to a predetermined position via the operation arm 7 as described above, so that the entire apparatus 1 can be operated in a vacuum environment or a nitrogen gas atmosphere. It is. That is, fully automatic operation that does not require human hands can be performed.
  • the milk teeth storage box 3 having an appropriate size may be a detachable cassette type. Moreover, although it may package separately using the operation arm 7, in any case, it will be sealed and will be filled with vacuum or nitrogen gas.
  • information related to “deciduous teeth” can be appropriately managed by using various barcodes and the like so that information can be shared between remote locations via a network.
  • biological tissues such as deciduous teeth collected according to various purposes can be stored / desired within a desired period while appropriately suppressing the propagation of miscellaneous fungi by a novel method that has not been conventionally used. Since it can be transported and managed, it has new possibilities in many fields and is an invention excellent in industrial applicability.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Hematology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention porte sur un nouveau système par lequel un tissu corporel, ou analogue, ayant été recueilli pour différents buts, peut être préservé, transporté et manipulé pendant une période de temps désirée tout en empêchant de façon adéquate la prolifération de micro-organismes contaminants. Un tissu corporel, ou analogue, est recueilli, puis maintenu dans une position prédéterminée dans une boîte de stockage (3) qui comporte un moyen de maintien et peut être scellée sous vide. Après un scellage hermétique, le vide est effectué dans la boîte de stockage (3) à un degré d'au moins -20 kPa environ afin d'empêcher la prolifération de différents micro-organismes contaminants, ou analogue, de façon à maintenir ainsi le tissu corporel, ou analogue, dans l'état tel qu'il était immédiatement après son recueil. Ensuite, l'espace interne de la boîte est rempli d'un gaz inerte tel qu'un gaz d’azote et scellé, si nécessaire. L'intérieur de la boîte de stockage est refroidi à une basse température prédéterminée, puis cette condition d’étanchéité et de refroidissement est maintenue grâce à un moyen approprié.
PCT/JP2008/070360 2008-10-31 2008-10-31 Système pour stérilisation hypoxique, préservation, transport et manipulation de tissu corporel devant être utilisé dans la médecine régénérative WO2010050073A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2008/070360 WO2010050073A1 (fr) 2008-10-31 2008-10-31 Système pour stérilisation hypoxique, préservation, transport et manipulation de tissu corporel devant être utilisé dans la médecine régénérative

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2008/070360 WO2010050073A1 (fr) 2008-10-31 2008-10-31 Système pour stérilisation hypoxique, préservation, transport et manipulation de tissu corporel devant être utilisé dans la médecine régénérative

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WO2010050073A1 true WO2010050073A1 (fr) 2010-05-06

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150000507A (ko) * 2012-04-13 2015-01-02 어플라이드 머티어리얼스, 인코포레이티드 망간 및 망간 니트라이드들의 증착 방법들
WO2016170281A1 (fr) * 2015-04-23 2016-10-27 Etablissement Francais Du Sang Procede de conservation de cellules, tissus ou organes en hypothermie
CN109625662A (zh) * 2019-01-11 2019-04-16 舟山医院 医用液氮自动存取装置
US11414647B2 (en) * 2014-08-25 2022-08-16 Simone Spuler Method for cultivating stem cells in vitro
US11589575B2 (en) 2015-09-10 2023-02-28 Etablissement Francais Du Sang Injectable preserving medium for preserving cells from placental blood, from bone marrow and from peripheral blood

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004290134A (ja) * 2003-03-28 2004-10-21 Hitachi Ltd 細胞の培養に関する管理方法および細胞の培養に関する管理システム
JP2004535240A (ja) * 2001-07-19 2004-11-25 ザ クリーブランド クリニック ファウンデーション 生物組織層を有する血管内代用物

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004535240A (ja) * 2001-07-19 2004-11-25 ザ クリーブランド クリニック ファウンデーション 生物組織層を有する血管内代用物
JP2004290134A (ja) * 2003-03-28 2004-10-21 Hitachi Ltd 細胞の培養に関する管理方法および細胞の培養に関する管理システム

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150000507A (ko) * 2012-04-13 2015-01-02 어플라이드 머티어리얼스, 인코포레이티드 망간 및 망간 니트라이드들의 증착 방법들
KR102189781B1 (ko) * 2012-04-13 2020-12-11 어플라이드 머티어리얼스, 인코포레이티드 망간 및 망간 니트라이드들의 증착 방법들
US11414647B2 (en) * 2014-08-25 2022-08-16 Simone Spuler Method for cultivating stem cells in vitro
WO2016170281A1 (fr) * 2015-04-23 2016-10-27 Etablissement Francais Du Sang Procede de conservation de cellules, tissus ou organes en hypothermie
FR3035407A1 (fr) * 2015-04-23 2016-10-28 Francais Du Sang Ets Procede de conservation de cellules, tissus ou organes en hypothermie
CN107872954A (zh) * 2015-04-23 2018-04-03 法国血液机构 在低温下保存细胞、组织或器官的方法
US10660329B2 (en) 2015-04-23 2020-05-26 Etablissement Francais Du Sang Method for preserving cells, tissues or organs in hypothermia
US11589575B2 (en) 2015-09-10 2023-02-28 Etablissement Francais Du Sang Injectable preserving medium for preserving cells from placental blood, from bone marrow and from peripheral blood
CN109625662A (zh) * 2019-01-11 2019-04-16 舟山医院 医用液氮自动存取装置

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