WO2010050073A1 - System for hypoxic sterilization, preservation, transportation and management of body tissue to be used in regenerative medicine - Google Patents
System for hypoxic sterilization, preservation, transportation and management of body tissue to be used in regenerative medicine Download PDFInfo
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- WO2010050073A1 WO2010050073A1 PCT/JP2008/070360 JP2008070360W WO2010050073A1 WO 2010050073 A1 WO2010050073 A1 WO 2010050073A1 JP 2008070360 W JP2008070360 W JP 2008070360W WO 2010050073 A1 WO2010050073 A1 WO 2010050073A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- the present invention is intended to propose a novel system capable of storing, transporting, and managing living tissue collected according to various purposes within a desired period while appropriately suppressing the propagation of miscellaneous fungi. is there.
- cryopreserving cryogenic conditions such as liquid nitrogen
- it is difficult to implement safely without a research institution with considerable equipment.
- cryogenicity such as liquid nitrogen is dangerous in itself, and considerable energy and cost are required for maintenance.
- the present invention has been devised in view of the above-described prior art, and has been devised through research and examination.
- the technical principle applied to the two-tank differential pressure dust-free cleaning device in a semiconductor wafer manufacturing apparatus is applied.
- By controlling the amount of oxygen around cells, tissues, and organs to be stored under conditions of vacuum and nitrogen gas or other inert gas atmosphere it effectively prevents the respiration necessary for the propagation of various fungi, and the decay progresses. It is proposed to prevent this, and more specifically is as follows.
- the obtained biological tissue or the like is held in a predetermined position of a storage box that is equipped with holding means and can be sealed in a vacuum state,
- the container is sealed and the vacuum state is evacuated to about ⁇ 20 Kpa or less after sealing,
- the storage box is filled and sealed with inert gas such as nitrogen gas as necessary, Cool the inside of the storage box to a predetermined low temperature state, Continue this sealed low-temperature state by appropriate means, If necessary, record the progress and continuation status of each procedure in the computer device along with the passage of time after acquisition of biological tissue, etc.
- a system for low-oxygen sterilization / storage / transport / management of biological tissue used for regenerative medicine characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after acquisition of the biological tissue.
- the extracted deciduous teeth are held in place in a storage box that is equipped with holding means and can be sealed in a vacuum state, In order to prevent the propagation of various germs and maintain and maintain the deciduous teeth state immediately after removal, the container is sealed and the vacuum state in which air is evacuated to at least ⁇ 20 Kpa is provided.
- the storage box is filled and sealed with inert gas such as nitrogen gas as necessary, Cool the inside of the storage box to a predetermined low temperature state, Continue this sealed low-temperature state by appropriate means, If necessary, record the progress and continuation status of each procedure in the computer device with the passage of time after extracting the deciduous teeth,
- inert gas such as nitrogen gas
- the portion containing the dental pulp cells at the tip of the deciduous teeth is protected without being damaged by the pressure contact / fixing means sandwiched from at least two sides. Hold the extracted deciduous teeth in a state that has a shape like a net or a bag and is detachable in a cassette-type device that is pressed and fixed again with tweezers as necessary.
- the extracted deciduous teeth are automatically and mechanically stored with arm-type vacuum tweezers in a low-oxygen storage room or a cooling storage room that can be controlled at -25 ° C or lower.
- the living tissue used for regenerative medicine according to any one of the above items (1) to (3), wherein the tissue can be transported without being exposed to air. Transportation and management system.
- each treatment in a clean state excluding fouling can be performed by washing the tissue surface with physiological saline or the like as long as the acquired biological tissue is not damaged.
- the obtained biological tissue etc. is held in place in a storage box equipped with holding means and sealed in a vacuum state to propagate various germs
- a storage box equipped with holding means and sealed in a vacuum state to propagate various germs
- it is included in the air by sealing the storage box and then evacuating it to at least -20 Kpa (kilopascal) or less. This also substantially reduces and removes the generated oxygen, thereby suppressing the growth of microorganisms such as miscellaneous bacteria that perform oxygen respiration.
- the storage box It becomes possible to continue to suppress the propagation of germs and the like more effectively while maintaining the inside in a positive pressure state that is easier to manage.
- an inert gas such as nitrogen gas
- a pedestal that is a cassette-type device, if necessary, holds the extracted milk teeth in a state where they are pressed and fixed again with tweezers, and is covered with cleaning means such as a cleaning nozzle that covers the entire pedestal.
- FIG. 1 is a flowchart showing a series of work steps performed by a system apparatus using the present invention.
- the door 2 of the system device 1 of the present invention is opened, held in a predetermined position of the storage box 3, set and then closed.
- FIG. 1 An example of the internal configuration of such an apparatus 1 is shown in FIG. That is, the milk teeth can be held, fixed, and moved by the operation arm 7 having the vacuum tweezers 6 attached to the tip while acquiring image information with the CCD camera 5. At this time, a hole may be made at a predetermined location with a drill 8 to facilitate collection of dental pulp tissue.
- the cleaning nozzle 9 may be covered from above to perform a necessary cleaning process, and the rotor 10 on which the milk teeth are installed may be dried at a low speed of about 100 revolutions per minute. With this low speed rotation, there is no risk of physical damage to the deciduous teeth.
- a vacuum state is established as a sealed state.
- oxygen necessary for the propagation of miscellaneous fungi is excluded from the vicinity of the deciduous teeth, and decay during storage and storage is prevented.
- the vacuum state is a negative pressure state, and the outside air will invade only when the sealed state is slightly impaired, but if it is refilled with a harmless inert gas such as nitrogen gas, the sealed state is temporarily Even if it is damaged, only the internal gas leaks out, and there is no possibility of outside air hitting the stored milk teeth.
- the deciduous teeth to be stored and transported are fixed and moved to a predetermined position via the operation arm 7 as described above, so that the entire apparatus 1 can be operated in a vacuum environment or a nitrogen gas atmosphere. It is. That is, fully automatic operation that does not require human hands can be performed.
- the milk teeth storage box 3 having an appropriate size may be a detachable cassette type. Moreover, although it may package separately using the operation arm 7, in any case, it will be sealed and will be filled with vacuum or nitrogen gas.
- information related to “deciduous teeth” can be appropriately managed by using various barcodes and the like so that information can be shared between remote locations via a network.
- biological tissues such as deciduous teeth collected according to various purposes can be stored / desired within a desired period while appropriately suppressing the propagation of miscellaneous fungi by a novel method that has not been conventionally used. Since it can be transported and managed, it has new possibilities in many fields and is an invention excellent in industrial applicability.
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Abstract
Proposed is a novel system whereby a body tissue or the like having been collected for various purposes can be preserved, transported and managed within a desired period of time while adequately preventing the proliferation of contaminating microorganisms. A body tissue or the like is collected and then held at a predetermined position in a storage box (3) which is provided with a holding means and vacuum-sealable. After tightly sealing, the storage box (3) is evacuated to a degree of at least about -20 KPa to prevent the proliferation of various contaminating microorganisms or the like thereby maintaining the body tissue or the like in the state immediately after collecting. Next, the inner space of the box is filled with an inert gas such as nitrogen gas and sealed, if required. The inside of the storage box is cooled to a predetermined low temperature and then this sealed and cooled state is continued by an appropriate means.
Description
この発明は、各種目的に応じて採取された生体組織などを雑菌類の繁殖を適切に抑制しながら所望の期間内で保存・運搬・管理することができる新規なシステムを提案しようとするものである。
The present invention is intended to propose a novel system capable of storing, transporting, and managing living tissue collected according to various purposes within a desired period while appropriately suppressing the propagation of miscellaneous fungi. is there.
半導体ウェハの製造装置技術として、エッチング工程や乾燥工程を適当な真空状態（低酸素状態）の特性を活かしながら、それにより作業効率を高めると共に多品種少量生産といった市場の要求にも適切に対応しようとする技術が存在している。
As a semiconductor wafer manufacturing equipment technology, while taking advantage of the characteristics of appropriate vacuum conditions (low oxygen conditions) in the etching process and drying process, we will improve work efficiency and respond appropriately to market demands such as high-mix low-volume production. Technology exists.
これは真空状態を利用した差圧式２槽構造の装置を用いて各槽間における迅速且つ確実なガス交換を行って無塵化処理や乾燥処理を簡易に行うものである。
¡This is a simple and easy process for dedusting and drying by exchanging gas between each tank quickly and surely using an apparatus with a differential pressure type two tank structure utilizing a vacuum state.
また、研究や実験、医療その他の目的で採取された各種生体組織を、液体窒素などを用いて極低温状態で長期間冷凍保存管理することも各種研究機関などにおいて普通に行われているところであり、特に近時は将来の再生医療に利用することを目的とした組織細胞の保存などに注目が集まってきている。
特許第３５４８９７６号
In addition, various types of biological tissues collected for research, experimentation, medical care and other purposes are usually stored in various research institutes for a long period of time by cryogenic conditions using liquid nitrogen. In particular, attention has recently been focused on the preservation of tissue cells intended for use in future regenerative medicine.
Japanese Patent No. 3548976
液体窒素などの極低温状態を冷凍保存する技術は確立されたものであるが、相当な設備を有する研究機関などでなければ安全に実施することは難しい。つまり、液体窒素のような極低温性はそれ自体において危険性があり、また、維持管理においても相当なエネルギーとコストが要求されることになってしまう。
Although technology for cryopreserving cryogenic conditions such as liquid nitrogen has been established, it is difficult to implement safely without a research institution with considerable equipment. In other words, cryogenicity such as liquid nitrogen is dangerous in itself, and considerable energy and cost are required for maintenance.
したがって、通常の病院や歯科医院などの機関でこれを行うことは不可能と言ってよく、また、極端な低温状態への移行は当然に生体組織へのある程度の損傷が不可避であることから、不安定要素を含んだ比較的短期間（２、３日間から２、３週間程度の期間）の運搬や保存に採用することは望ましくない。
Therefore, it can be said that it is impossible to do this in an organization such as a normal hospital or dental clinic, and since the transition to an extremely low temperature state is naturally unavoidable to some degree of damage to living tissue, It is not desirable to adopt it for transportation and storage for a relatively short period of time (a few days to a few weeks) including unstable elements.
近時は、生物学的技術や研究内容の進展に伴って野外等を含んだ多くの一般的な場所でも各種の動物や植物の組織を採取し、場合によっては生きたままの状態を維持しながら保存・運搬することが望まれたり、普通の歯科医院や医療機関においても何らかの理由で採取された細胞や組織、器官の一部などを、可能な限り損傷を与えないでそのままの状態で、専門の研究機関に所定の期間内に運搬しなければならない、といった要請も生まれてきている。
In recent years, various animal and plant tissues have been collected in many general places, including the outdoors, as biological technology and research progressed, and in some cases they can be kept alive. However, it is desirable to store and transport the cells, tissues, and parts of organs collected for some reason even in ordinary dental clinics and medical institutions, without damaging them as much as possible. There has also been a demand for transport to specialized research institutions within a specified period.
採取された細胞や組織、器官などの損傷原因としては、雑菌類の繁殖による腐敗が大きなものとしてあるが、これを物理的な損傷を与えずに防止する方法としては低温冷凍しかないのが実情である。
したがって、冷凍保存方法以外に細胞や組織、器官などを物理的に損傷せず雑菌類の繁殖に起因した腐敗などを適切に阻止することが比較的簡易な設備で低エネルギーかつ低コストに実現されることが望まれているのである。 As a cause of damage to collected cells, tissues, organs, etc., spoilage due to breeding of miscellaneous fungi is considered to be a major cause, but the only way to prevent this without physically damaging is the fact that there is only low temperature freezing. It is.
Therefore, in addition to the cryopreservation method, it is possible to appropriately prevent rot caused by the propagation of miscellaneous bacteria without physically damaging cells, tissues, organs, etc. with low energy and low cost. It is hoped that
したがって、冷凍保存方法以外に細胞や組織、器官などを物理的に損傷せず雑菌類の繁殖に起因した腐敗などを適切に阻止することが比較的簡易な設備で低エネルギーかつ低コストに実現されることが望まれているのである。 As a cause of damage to collected cells, tissues, organs, etc., spoilage due to breeding of miscellaneous fungi is considered to be a major cause, but the only way to prevent this without physically damaging is the fact that there is only low temperature freezing. It is.
Therefore, in addition to the cryopreservation method, it is possible to appropriately prevent rot caused by the propagation of miscellaneous bacteria without physically damaging cells, tissues, organs, etc. with low energy and low cost. It is hoped that
本発明は上記したような従来技術に鑑みて研究・検討を重ねて創案されたものであり、半導体ウェハの製造装置における二槽式差圧無塵洗浄装置に採用された技術原理を応用して保存すべき細胞や組織、器官の周囲の酸素量を真空状態や窒素ガスその他の不活性ガス雰囲気の条件を管理することで、雑菌類の繁殖に必要な呼吸を事実上阻止して腐敗が進行することを防止することを提案するものであって、より具体的には以下の如くである。
The present invention has been devised in view of the above-described prior art, and has been devised through research and examination. The technical principle applied to the two-tank differential pressure dust-free cleaning device in a semiconductor wafer manufacturing apparatus is applied. By controlling the amount of oxygen around cells, tissues, and organs to be stored under conditions of vacuum and nitrogen gas or other inert gas atmosphere, it effectively prevents the respiration necessary for the propagation of various fungi, and the decay progresses. It is proposed to prevent this, and more specifically is as follows.
（１）半導体ウェハの製造装置における二槽式差圧無塵洗浄装置に採用された技術原理を再生医療における分野のための生体組織の取得・保存・運搬・滅菌等における処置において利用して健全な状態で長期間冷凍保存する施設に運ぶに際し、
生体組織等の取得処置を行う各種施設などにおいて、適正な対象組織付近の滅菌処理を行ったことを確認した上で所定の生体組織等の取得を行い、
取得した生体組織等に関する取得処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録し、
必要に応じて取得した生体組織等を損傷しない範囲で組織表面を生理食塩水などで洗浄し、
必要に応じて充分に滅菌された保護手段で被包した後に、取得した生体組織等を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、
各種雑菌などの繁殖を阻止して取得処置直後の生体組織等の状態を維持保存するために、収納箱を密封後に少なくとも−２０Ｋｐａ以下程度に空気を抜いた真空状態となし、
その後必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、
収納箱内を所定の低温状態にまで冷却し、
この密封低温状態を適宜な手段により継続させ、
必要に応じて上記各手順の経過および継続状況を生体組織等の取得後の時間経過と共に上記コンピュータ装置に入力記録し、
生体組織等の取得後所定の時間以内に長期間冷凍保存施設に運搬できるようにしたことを特徴とする再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 (1) Utilize the technical principle adopted in the two-tank differential pressure dust-free cleaning device in semiconductor wafer manufacturing equipment in procedures for the acquisition, storage, transport, and sterilization of biological tissues for the field of regenerative medicine. When transporting to a facility that is frozen and stored for a long period of time,
After confirming that sterilization treatment near the appropriate target tissue has been performed in various facilities that perform acquisition procedures such as biological tissue, perform acquisition of predetermined biological tissue,
Input and record basic information including time information that indicates the passage of time after the acquisition procedure related to the acquired biological tissue, etc., to a computer device connectable to the network,
If necessary, wash the tissue surface with physiological saline in a range that does not damage the acquired biological tissue, etc.
After encapsulating with protective means sufficiently sterilized as necessary, the obtained biological tissue or the like is held in a predetermined position of a storage box that is equipped with holding means and can be sealed in a vacuum state,
In order to prevent the propagation of various germs and maintain and preserve the state of the biological tissue etc. immediately after the acquisition treatment, the container is sealed and the vacuum state is evacuated to about −20 Kpa or less after sealing,
After that, the storage box is filled and sealed with inert gas such as nitrogen gas as necessary,
Cool the inside of the storage box to a predetermined low temperature state,
Continue this sealed low-temperature state by appropriate means,
If necessary, record the progress and continuation status of each procedure in the computer device along with the passage of time after acquisition of biological tissue, etc.
A system for low-oxygen sterilization / storage / transport / management of biological tissue used for regenerative medicine, characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after acquisition of the biological tissue.
生体組織等の取得処置を行う各種施設などにおいて、適正な対象組織付近の滅菌処理を行ったことを確認した上で所定の生体組織等の取得を行い、
取得した生体組織等に関する取得処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録し、
必要に応じて取得した生体組織等を損傷しない範囲で組織表面を生理食塩水などで洗浄し、
必要に応じて充分に滅菌された保護手段で被包した後に、取得した生体組織等を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、
各種雑菌などの繁殖を阻止して取得処置直後の生体組織等の状態を維持保存するために、収納箱を密封後に少なくとも−２０Ｋｐａ以下程度に空気を抜いた真空状態となし、
その後必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、
収納箱内を所定の低温状態にまで冷却し、
この密封低温状態を適宜な手段により継続させ、
必要に応じて上記各手順の経過および継続状況を生体組織等の取得後の時間経過と共に上記コンピュータ装置に入力記録し、
生体組織等の取得後所定の時間以内に長期間冷凍保存施設に運搬できるようにしたことを特徴とする再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 (1) Utilize the technical principle adopted in the two-tank differential pressure dust-free cleaning device in semiconductor wafer manufacturing equipment in procedures for the acquisition, storage, transport, and sterilization of biological tissues for the field of regenerative medicine. When transporting to a facility that is frozen and stored for a long period of time,
After confirming that sterilization treatment near the appropriate target tissue has been performed in various facilities that perform acquisition procedures such as biological tissue, perform acquisition of predetermined biological tissue,
Input and record basic information including time information that indicates the passage of time after the acquisition procedure related to the acquired biological tissue, etc., to a computer device connectable to the network,
If necessary, wash the tissue surface with physiological saline in a range that does not damage the acquired biological tissue, etc.
After encapsulating with protective means sufficiently sterilized as necessary, the obtained biological tissue or the like is held in a predetermined position of a storage box that is equipped with holding means and can be sealed in a vacuum state,
In order to prevent the propagation of various germs and maintain and preserve the state of the biological tissue etc. immediately after the acquisition treatment, the container is sealed and the vacuum state is evacuated to about −20 Kpa or less after sealing,
After that, the storage box is filled and sealed with inert gas such as nitrogen gas as necessary,
Cool the inside of the storage box to a predetermined low temperature state,
Continue this sealed low-temperature state by appropriate means,
If necessary, record the progress and continuation status of each procedure in the computer device along with the passage of time after acquisition of biological tissue, etc.
A system for low-oxygen sterilization / storage / transport / management of biological tissue used for regenerative medicine, characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after acquisition of the biological tissue.
（２）半導体ウェハの製造装置における二槽式差圧無塵洗浄装置に採用された技術原理を再生医療における分野のための乳歯幹細胞組織の取得・保存・運搬・滅菌等における処置において利用して健全な状態で長期間冷凍保存する施設に運ぶに際し、
抜去処置を行う歯科医院などの施設において、適正な口腔内滅菌処理を行ったことを確認した上で所定の乳歯を抜去し、
抜去した乳歯に関する抜去処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録し、
必要に応じて歯髄組織を損傷しない範囲で抜去乳歯表面を生理食塩水などで洗浄し、
必要に応じて充分に滅菌された保護手段で被包した後に、抜去乳歯を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、
各種雑菌などの繁殖を阻止して抜去直後の乳歯状態を維持保存するために、収納箱を密封後に少なくとも−２０Ｋｐａに空気を抜いた真空状態となし、
その後必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、
収納箱内を所定の低温状態にまで冷却し、
この密封低温状態を適宜な手段により継続させ、
必要に応じて上記各手順の経過および継続状況を乳歯抜去後の時間経過と共に上記コンピュータ装置に入力記録し、
乳歯抜去後所定の時間以内に長期間冷凍保存施設に運搬できるようにしたことを特徴とする再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 (2) Utilizing the technical principle adopted in the two-tank differential pressure dust-free cleaning device in semiconductor wafer manufacturing equipment in the treatment of acquisition, storage, transport and sterilization of deciduous stem cell tissue for the field of regenerative medicine When transporting to a facility that is kept frozen for a long time in a healthy state,
After confirming that proper oral sterilization has been performed at a facility such as a dental clinic where the extraction procedure is performed, the prescribed milk teeth are extracted,
Input and record basic information including time information about the time elapsed after the extraction procedure for the extracted milk teeth to a computer device that can be connected to the network.
If necessary, remove the surface of the deciduous tooth as long as it does not damage the pulp tissue.
After encapsulating with protective means sufficiently sterilized as necessary, the extracted deciduous teeth are held in place in a storage box that is equipped with holding means and can be sealed in a vacuum state,
In order to prevent the propagation of various germs and maintain and maintain the deciduous teeth state immediately after removal, the container is sealed and the vacuum state in which air is evacuated to at least −20 Kpa is provided.
After that, the storage box is filled and sealed with inert gas such as nitrogen gas as necessary,
Cool the inside of the storage box to a predetermined low temperature state,
Continue this sealed low-temperature state by appropriate means,
If necessary, record the progress and continuation status of each procedure in the computer device with the passage of time after extracting the deciduous teeth,
A system for low-oxygen sterilization, storage, transport, and management of living tissue used for regenerative medicine, characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after removal of a deciduous tooth.
抜去処置を行う歯科医院などの施設において、適正な口腔内滅菌処理を行ったことを確認した上で所定の乳歯を抜去し、
抜去した乳歯に関する抜去処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録し、
必要に応じて歯髄組織を損傷しない範囲で抜去乳歯表面を生理食塩水などで洗浄し、
必要に応じて充分に滅菌された保護手段で被包した後に、抜去乳歯を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、
各種雑菌などの繁殖を阻止して抜去直後の乳歯状態を維持保存するために、収納箱を密封後に少なくとも−２０Ｋｐａに空気を抜いた真空状態となし、
その後必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、
収納箱内を所定の低温状態にまで冷却し、
この密封低温状態を適宜な手段により継続させ、
必要に応じて上記各手順の経過および継続状況を乳歯抜去後の時間経過と共に上記コンピュータ装置に入力記録し、
乳歯抜去後所定の時間以内に長期間冷凍保存施設に運搬できるようにしたことを特徴とする再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 (2) Utilizing the technical principle adopted in the two-tank differential pressure dust-free cleaning device in semiconductor wafer manufacturing equipment in the treatment of acquisition, storage, transport and sterilization of deciduous stem cell tissue for the field of regenerative medicine When transporting to a facility that is kept frozen for a long time in a healthy state,
After confirming that proper oral sterilization has been performed at a facility such as a dental clinic where the extraction procedure is performed, the prescribed milk teeth are extracted,
Input and record basic information including time information about the time elapsed after the extraction procedure for the extracted milk teeth to a computer device that can be connected to the network.
If necessary, remove the surface of the deciduous tooth as long as it does not damage the pulp tissue.
After encapsulating with protective means sufficiently sterilized as necessary, the extracted deciduous teeth are held in place in a storage box that is equipped with holding means and can be sealed in a vacuum state,
In order to prevent the propagation of various germs and maintain and maintain the deciduous teeth state immediately after removal, the container is sealed and the vacuum state in which air is evacuated to at least −20 Kpa is provided.
After that, the storage box is filled and sealed with inert gas such as nitrogen gas as necessary,
Cool the inside of the storage box to a predetermined low temperature state,
Continue this sealed low-temperature state by appropriate means,
If necessary, record the progress and continuation status of each procedure in the computer device with the passage of time after extracting the deciduous teeth,
A system for low-oxygen sterilization, storage, transport, and management of living tissue used for regenerative medicine, characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after removal of a deciduous tooth.
（３）乳歯を抜去後、少なくとも２方向以上の側面から挟み込んでの圧接・固定手段により乳歯の先端部分の歯髄細胞が含まれる部分を損傷することなく保護し、
網や籠のような形状とし、取り外し自在なカセット式装置とした台座に必要に応じてピンセットで再度圧接・固定した状態として抜去した乳歯を保持し、
上記台座全体を覆う洗浄用ノズルのような洗浄手段を被せ、
次段階として装置上蓋を閉じて密閉状態とし、
真空ポンプを作動させて装置の内部を−２０Ｋｐａ以下程度の真空状態にし、
その後、ローターを毎分１００回転程度の低速で回転させて、
さらに、必要に応じて生理食塩水、食塩水・オゾン水のような機能水や純水で洗浄し、さらにその後、低酸素状態で乳歯を保持したままのカセット式装置を取り外して運搬することを特徴とする前記（１）項又は（２）項に記載の再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 (3) After removing the deciduous teeth, the portion containing the dental pulp cells at the tip of the deciduous teeth is protected without being damaged by the pressure contact / fixing means sandwiched from at least two sides.
Hold the extracted deciduous teeth in a state that has a shape like a net or a bag and is detachable in a cassette-type device that is pressed and fixed again with tweezers as necessary.
Cover the entire pedestal with a cleaning means such as a cleaning nozzle,
As the next step, close the top lid of the device to make it sealed,
Activate the vacuum pump to bring the inside of the device into a vacuum state of about -20 Kpa or less,
Then, rotate the rotor at a low speed of about 100 revolutions per minute,
Furthermore, if necessary, wash with functional water or pure water such as physiological saline, saline / ozone water, and then remove and transport the cassette-type device holding the deciduous teeth in a low oxygen state. A system for low oxygen sterilization / preservation / transport / management of biological tissue or the like used for regenerative medicine according to (1) or (2), which is characterized in the above.
網や籠のような形状とし、取り外し自在なカセット式装置とした台座に必要に応じてピンセットで再度圧接・固定した状態として抜去した乳歯を保持し、
上記台座全体を覆う洗浄用ノズルのような洗浄手段を被せ、
次段階として装置上蓋を閉じて密閉状態とし、
真空ポンプを作動させて装置の内部を−２０Ｋｐａ以下程度の真空状態にし、
その後、ローターを毎分１００回転程度の低速で回転させて、
さらに、必要に応じて生理食塩水、食塩水・オゾン水のような機能水や純水で洗浄し、さらにその後、低酸素状態で乳歯を保持したままのカセット式装置を取り外して運搬することを特徴とする前記（１）項又は（２）項に記載の再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 (3) After removing the deciduous teeth, the portion containing the dental pulp cells at the tip of the deciduous teeth is protected without being damaged by the pressure contact / fixing means sandwiched from at least two sides.
Hold the extracted deciduous teeth in a state that has a shape like a net or a bag and is detachable in a cassette-type device that is pressed and fixed again with tweezers as necessary.
Cover the entire pedestal with a cleaning means such as a cleaning nozzle,
As the next step, close the top lid of the device to make it sealed,
Activate the vacuum pump to bring the inside of the device into a vacuum state of about -20 Kpa or less,
Then, rotate the rotor at a low speed of about 100 revolutions per minute,
Furthermore, if necessary, wash with functional water or pure water such as physiological saline, saline / ozone water, and then remove and transport the cassette-type device holding the deciduous teeth in a low oxygen state. A system for low oxygen sterilization / preservation / transport / management of biological tissue or the like used for regenerative medicine according to (1) or (2), which is characterized in the above.
（４）抜去した乳歯をアーム式真空ピンセットで自動的・機械的に、低酸素保管庫や−２５℃以下に管理可能な冷却用保管庫に入れて運搬することで、雑菌類を含んだ外部の空気に触れずに運搬することが可能となることを特徴とする前記（１）項~（３）項の何れか一つに記載の再生医療に利用する生体組織の低酸素滅菌・保存・運搬・管理用システム。
(4) The extracted deciduous teeth are automatically and mechanically stored with arm-type vacuum tweezers in a low-oxygen storage room or a cooling storage room that can be controlled at -25 ° C or lower. The living tissue used for regenerative medicine according to any one of the above items (1) to (3), wherein the tissue can be transported without being exposed to air. Transportation and management system.
（５）ＣＣＤカメラなどの映像記録手段によって歯髄の位置や汚れ状況を確認し、歯髄を採りやすくするためにダイヤモンドドリルのような開穴手段で乳歯の上部に穴を開け、一方で、歯根側から圧縮空気を入れることで押しだされてきた歯髄組織を、ＣＣＤカメラの映像を確認しながらマニピュレータなどにより人手を直接触れさせないで採取するようにしたことを特徴とする前記（１）項~（４）項の何れか１つに記載の再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。
(5) Check the position and dirt of the pulp with video recording means such as a CCD camera, and make a hole in the upper part of the deciduous tooth with an opening means such as a diamond drill to make it easier to pick up the pulp. (1) to (1) above, wherein the pulp tissue pushed out by compressed air is collected without directly touching a manipulator with a manipulator while confirming the image of the CCD camera. A system for low oxygen sterilization / preservation / transport / management of biological tissue used for regenerative medicine according to any one of 4).
生体組織等の取得処置を行う各種施設などにおいて、適正な対象組織付近の滅菌処理を行ったことを確認した上で所定の生体組織等の取得を行うことで、腐敗原因となる雑菌類を十分に除去した状態での処置が可能となる。
In various facilities that acquire living tissue, etc., confirm that sterilization near the appropriate target tissue has been performed, and then acquire the specified biological tissue, etc. It is possible to perform the treatment in the state where it is removed.
取得した生体組織等に関する取得処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録することで、将来に渡って適切なデータ管理を行う基礎情報を得ることができる。
Obtain basic information for appropriate data management in the future by inputting and recording basic information including time information about the acquired biological tissue, etc. after the acquisition procedure into a computer device that can be connected to the network. be able to.
必要に応じて取得した生体組織等を損傷しない範囲で組織表面を生理食塩水などで洗浄することで、汚損などを除いた清浄な状態での各処理が可能となる。
If necessary, each treatment in a clean state excluding fouling can be performed by washing the tissue surface with physiological saline or the like as long as the acquired biological tissue is not damaged.
必要に応じて充分に滅菌された保護手段で被包した後に、取得した生体組織等を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、各種雑菌などの繁殖を阻止して取得処置直後の生体組織等の状態を維持保存するために、収納箱を密封後に少なくとも−２０Ｋｐａ（キロパスカル）以下程度に空気を抜いた真空状態となすことで、空気中に含まれた酸素をも実質的に低減・除去することになり、酸素呼吸を行う雑菌類などの微生物の繁殖を抑制することができるようになる。
After encapsulating with adequately sterilized protection means as necessary, the obtained biological tissue etc. is held in place in a storage box equipped with holding means and sealed in a vacuum state to propagate various germs In order to prevent and maintain the state of the living tissue immediately after the acquisition procedure, it is included in the air by sealing the storage box and then evacuating it to at least -20 Kpa (kilopascal) or less. This also substantially reduces and removes the generated oxygen, thereby suppressing the growth of microorganisms such as miscellaneous bacteria that perform oxygen respiration.
必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、収納箱内を所定の低温状態にまで冷却し、この密封低温状態を適宜な手段により継続させることで、収納箱内をより管理の行いやすい正圧状態で維持しながら一層効果的に雑菌類などの繁殖を抑制し続けることが可能となる。
Filling and sealing the inside of the storage box with an inert gas such as nitrogen gas as necessary, cooling the inside of the storage box to a predetermined low temperature state, and continuing this sealed low temperature state by appropriate means, the storage box It becomes possible to continue to suppress the propagation of germs and the like more effectively while maintaining the inside in a positive pressure state that is easier to manage.
抜去した乳歯の歯髄組織の保存・運搬に使用できるようにすることで、該組織に含まれる乳歯幹細胞を長期保存して将来培養して再生医療などに活用することが可能になる。
By making it possible to store and transport the pulp tissue of the extracted deciduous tooth, it becomes possible to store the deciduous stem cell contained in the tissue for a long period of time and culture it for future use in regenerative medicine.
乳歯を抜去後、少なくとも２方向以上の側面から挟み込んでの圧接・固定手段により乳歯の先端部分の歯髄細胞が含まれる部分を損傷することなく保護し、網や籠のような形状とし、取り外し自在なカセット式装置とした台座に必要に応じてピンセットで再度圧接・固定した状態として抜去した乳歯を保持し、上記台座全体を覆う洗浄用ノズルのような洗浄手段を被せ、次段階として装置上蓋を閉じて密閉状態とし、真空ポンプを作動させて装置の内部を−２０Ｋｐａ（キロパスカル）以下程度の真空状態にし、その後、ローターを毎分１００回転程度の低速で回転させて、さらに、必要に応じて生理食塩水、食塩水・オゾン水のような機能水や純水で洗浄し、さらにその後、低酸素状態で乳歯を保持したままのカセット式装置を取り外して運搬することで、可能な限り雑菌類の付着を阻止しながら歯髄細胞の保存・運搬に特化した最適のシステムとすることが可能となる。
After removing the deciduous teeth, the part containing the pulp cells at the tip of the deciduous teeth is protected without damage by pressing and fixing means sandwiched from at least two sides. A pedestal that is a cassette-type device, if necessary, holds the extracted milk teeth in a state where they are pressed and fixed again with tweezers, and is covered with cleaning means such as a cleaning nozzle that covers the entire pedestal. Close and seal, operate the vacuum pump to bring the inside of the device to a vacuum of about -20 Kpa (kilopascals), then rotate the rotor at a low speed of about 100 revolutions per minute, and if necessary Wash with functional water or pure water such as physiological saline, saline / ozone water, and then remove the cassette-type device that retains the teeth in a hypoxic state. By carrying, it is possible to optimize the system dedicated to storage and transport of pulp cells while preventing adhesion as much bacteria such as possible.
抜去した乳歯をアーム式真空ピンセットで自動的・機械的に、低酸素保管庫や−２５℃以下に管理可能な冷却用保管庫に入れて運搬することで、雑菌類を含んだ外部の空気に触れずに運搬することが可能となることで、一連の作業をまったく人手にも触れないようにしながら行うことが可能となり、ヒューマンエラーに起因する問題発生をも適切に防止することになる。
Removed deciduous teeth automatically and mechanically with arm-type vacuum tweezers and transported in a low-oxygen storage room or a cooling storage room that can be controlled at -25 ° C or lower, to the outside air containing germs By being able to carry without touching it, it becomes possible to carry out a series of operations without touching human hands at all, and appropriately prevent problems caused by human errors.
ＣＣＤカメラなどの映像記録手段によって歯髄の位置や汚れ状況を確認し、歯髄を採りやすくするためにダイヤモンドドリルのような開穴手段で乳歯の上部に穴を開け、一方で、歯根側から圧縮空気を入れることで押しだされてきた歯髄組織を、ＣＣＤカメラの映像を確認しながらマニピュレータなどにより人手を直接触れさせないで採取するようにしたことで、真空状態や窒素ガスなどの充填状態であっても適宜な作業を行うことが可能となる。
Check the position and dirt of the pulp with video recording means such as a CCD camera, and make a hole in the upper part of the deciduous tooth with an opening means such as a diamond drill to make it easier to pick the pulp, while compressed air from the root side The dental pulp tissue that has been pushed out by putting in can be collected without touching it directly with a manipulator while checking the image of the CCD camera. Also, it is possible to perform appropriate work.
上記したような本発明の具体的な実施形態の若干例を、添付図面を利用しながら以下説明する。
図１は、本発明を用いたシステム装置による一連の作業工程をフローチャート的に示したものである。 Some examples of specific embodiments of the present invention as described above will be described below with reference to the accompanying drawings.
FIG. 1 is a flowchart showing a series of work steps performed by a system apparatus using the present invention.
図１は、本発明を用いたシステム装置による一連の作業工程をフローチャート的に示したものである。 Some examples of specific embodiments of the present invention as described above will be described below with reference to the accompanying drawings.
FIG. 1 is a flowchart showing a series of work steps performed by a system apparatus using the present invention.
すなわち、１例として、歯科医院において抜去された乳歯を保存・運搬する場合の説明を行う。まず、本発明のシステム装置１のトビラ２を開き、収容箱３の所定位置に保持させてからセッティングしてトビラ２を閉じる。
That is, as an example, explanation will be given for the case of storing and transporting milk teeth extracted in a dental clinic. First, the door 2 of the system device 1 of the present invention is opened, held in a predetermined position of the storage box 3, set and then closed.
以降の洗浄や真空化、窒素ガスなどの不活性ガスの充填、必要に応じた乾燥処理、低温化、収納箱３の密閉などは何れもこの装置１内で行われ、例えば操作パネル４などを介して行うものである。したがって、この間に人間の手が「乳歯」に触れることはなく、また、密閉後は外界の空気からも遮断されることになる。
Subsequent cleaning, evacuation, filling with an inert gas such as nitrogen gas, drying treatment as necessary, lowering the temperature, sealing the storage box 3, etc. are all carried out in this apparatus 1, for example, the operation panel 4 or the like. It is what you do through. Therefore, the human hand does not touch the “milk teeth” during this time, and after sealing, it is also shielded from the outside air.
このような装置１の内部構成の１例を図２に示す。すなわち、ＣＣＤカメラ５で画像情報を取得しながら真空ピンセット６を先端に取付けた操作アーム７で乳歯の保持・固定や移動を行うことができる。このとき、ドリル８により所定箇所に穴を開けて歯髄組織を採取し易くしておいてもよい。
An example of the internal configuration of such an apparatus 1 is shown in FIG. That is, the milk teeth can be held, fixed, and moved by the operation arm 7 having the vacuum tweezers 6 attached to the tip while acquiring image information with the CCD camera 5. At this time, a hole may be made at a predetermined location with a drill 8 to facilitate collection of dental pulp tissue.
また、洗浄ノズル９を上から被せて必要とする洗浄処理を行い、乳歯を設置しているローター１０を毎分１００回転程度の低速回転で乾燥処理してもよい。この程度の低速回転であれば乳歯に対する物理的損傷を与えるおそれがないのである。
Alternatively, the cleaning nozzle 9 may be covered from above to perform a necessary cleaning process, and the rotor 10 on which the milk teeth are installed may be dried at a low speed of about 100 revolutions per minute. With this low speed rotation, there is no risk of physical damage to the deciduous teeth.
さらにこの状態で密閉状態として真空状態とする。これにより雑菌類の繁殖に必要な酸素が乳歯周辺から排除されることになり、保存・保管中の腐敗が阻止される。また、真空状態は負圧状態であり、密閉状態が僅かに損なわれただけで外気が侵入してしまうが、窒素ガスなどの無害な不活性ガスを充填し直すようにすれば、仮に密閉状態が損なわれても内部ガスが漏れ出すだけであり、収納した乳歯に外気が当るおそれはなくなる。
Furthermore, in this state, a vacuum state is established as a sealed state. As a result, oxygen necessary for the propagation of miscellaneous fungi is excluded from the vicinity of the deciduous teeth, and decay during storage and storage is prevented. In addition, the vacuum state is a negative pressure state, and the outside air will invade only when the sealed state is slightly impaired, but if it is refilled with a harmless inert gas such as nitrogen gas, the sealed state is temporarily Even if it is damaged, only the internal gas leaks out, and there is no possibility of outside air hitting the stored milk teeth.
また、必要に応じて、少なくとも乳歯収納部付近を適当な低温状態に管理して併せて雑菌類の繁殖を抑制することも好ましい。保存・運搬する乳歯の所定位置への固定や移動は人手によらずに前記したように操作アーム７を介して行うので、装置１全体を真空環境下や窒素ガス雰囲気下で稼動させることも可能である。すなわち、人間の手を要しない全自動操作ができるのである。
In addition, if necessary, it is also preferable to control at least the vicinity of the deciduous tooth storage part to an appropriate low temperature state and to suppress the propagation of miscellaneous fungi. As described above, the deciduous teeth to be stored and transported are fixed and moved to a predetermined position via the operation arm 7 as described above, so that the entire apparatus 1 can be operated in a vacuum environment or a nitrogen gas atmosphere. It is. That is, fully automatic operation that does not require human hands can be performed.
適当なサイズとされた乳歯収容箱３は着脱自在なカセット式のものとしても良い。また、操作アーム７を使いながら別個にパッケージしてもよいが、何れにしても密閉して真空または窒素ガス等の充填状態とする。
The milk teeth storage box 3 having an appropriate size may be a detachable cassette type. Moreover, although it may package separately using the operation arm 7, in any case, it will be sealed and will be filled with vacuum or nitrogen gas.
また、各種バーコードなどを利用して「乳歯」に関する情報を適切にコンピュータ管理可能なものとし、ネットワークを介して遠隔地間などでも情報共有できるようにすることが好ましい。
In addition, it is preferable that information related to “deciduous teeth” can be appropriately managed by using various barcodes and the like so that information can be shared between remote locations via a network.
上記したような本発明によれば、各種目的に応じて採取された乳歯などの生体組織などを雑菌類の繁殖を従来にはない新規な手法で適切に抑制しながら所望の期間内で保存・運搬・管理することができるので、多方面に新たな可能性を秘めたものであり産業上の利用可能性において優れた発明である。
According to the present invention as described above, biological tissues such as deciduous teeth collected according to various purposes can be stored / desired within a desired period while appropriately suppressing the propagation of miscellaneous fungi by a novel method that has not been conventionally used. Since it can be transported and managed, it has new possibilities in many fields and is an invention excellent in industrial applicability.
５ カメラ移送用ボールスクリュウ
６ カメラ作動用モーター
７ スライドレール
８ 真空ポンプへ
９ バーコード読み取り
１０ 制御部
１１ ローダー部
１２ マイクロモーター
１３ ＣＣＤカメラ
１４ キリ
１５ 真空ピンセット
１６ 排水
１７ ダイヤモンドリル
１８ 冷却口
１９ 処理部
２０ ノズル
２１ 純水
２２ タンク
２３ ローター
２４ ローター移転用モーター
２５ アンローダー部
２６ パッケージ装着器
２７ パソコン 5 Ball screw forcamera transfer 6 Camera operation motor 7 Slide rail 8 To vacuum pump 9 Bar code reading 10 Control unit 11 Loader unit 12 Micro motor 13 CCD camera 14 Drill 15 Vacuum tweezers 16 Drain 17 Diamond Ril 18 Cooling port 19 Processing unit 20 Nozzle 21 Pure water 22 Tank 23 Rotor 24 Motor for rotor transfer 25 Unloader part 26 Package mounter 27 PC
６ カメラ作動用モーター
７ スライドレール
８ 真空ポンプへ
９ バーコード読み取り
１０ 制御部
１１ ローダー部
１２ マイクロモーター
１３ ＣＣＤカメラ
１４ キリ
１５ 真空ピンセット
１６ 排水
１７ ダイヤモンドリル
１８ 冷却口
１９ 処理部
２０ ノズル
２１ 純水
２２ タンク
２３ ローター
２４ ローター移転用モーター
２５ アンローダー部
２６ パッケージ装着器
２７ パソコン 5 Ball screw for
Claims (5)
- 半導体ウェハの製造装置における二槽式差圧無塵洗浄装置に採用された技術原理を再生医療における分野のための生体組織の取得・保存・運搬・滅菌等における処置において利用して健全な状態で長期間冷凍保存する施設に運ぶに際し、
生体組織等の取得処置を行う各種施設などにおいて、適正な対象組織付近の滅菌処理を行ったことを確認した上で所定の生体組織等の取得を行い、
取得した生体組織に関する取得処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録し、
必要に応じて取得した生体組織等を損傷しない範囲で組織表面を生理食塩水などで洗浄し、
必要に応じて充分に滅菌された保護手段で被包した後に、取得した生体組織等を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、
各種雑菌などの繁殖を阻止して取得処置直後の生体組織等の状態を維持保存するために、収納箱を密封後に空気を抜いた真空状態となし、
その後必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、
収納箱内を所定の低温状態にまで冷却し、
この密封低温状態を適宜な手段により継続させ、
必要に応じて上記各手順の経過および継続状況を生体組織等の取得後の時間経過と共に上記コンピュータ装置に入力記録し、
生体組織等の取得後所定の時間以内に長期間冷凍保存施設に運搬できるようにしたことを特徴とする再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 Utilizing the technical principles adopted in the two-tank differential pressure dust-free cleaning system in semiconductor wafer manufacturing equipment in procedures for acquiring, storing, transporting, and sterilizing living tissues for the field of regenerative medicine When transporting to a facility that is frozen for a long period of time,
After confirming that sterilization treatment near the appropriate target tissue has been performed in various facilities that perform acquisition procedures such as biological tissue, perform acquisition of predetermined biological tissue,
Input and record basic information including time information indicating the passage of time after the acquisition procedure related to the acquired biological tissue to a computer device connectable to the network,
If necessary, wash the tissue surface with physiological saline in a range that does not damage the acquired biological tissue, etc.
After encapsulating with protective means sufficiently sterilized as necessary, the obtained biological tissue or the like is held in a predetermined position of a storage box that is equipped with holding means and can be sealed in a vacuum state,
In order to prevent the propagation of various germs and maintain and preserve the state of biological tissue etc. immediately after the acquisition procedure, the storage box is sealed and the vacuum state is released,
After that, the storage box is filled and sealed with inert gas such as nitrogen gas as necessary,
Cool the inside of the storage box to a predetermined low temperature state,
Continue this sealed low-temperature state by appropriate means,
If necessary, record the progress and continuation status of each procedure in the computer device along with the passage of time after acquisition of biological tissue, etc.
A system for low-oxygen sterilization / storage / transport / management of biological tissue used for regenerative medicine, characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after acquisition of the biological tissue. - 半導体ウェハの製造装置における二槽式差圧無塵洗浄装置に採用された技術原理を再生医療における分野のための乳歯幹細胞組織の取得・保存・運搬・滅菌等における処置において利用して健全な状態で長期間冷凍保存する施設に運ぶに際し、
抜去処置を行う歯科医院などの施設において、適正な口腔内滅菌処理を行ったことを確認した上で所定の乳歯を抜去し、
抜去した乳歯に関する抜去処置後の時間経過が判る時間情報を含んだ基本情報をネットワークに接続可能なコンピュータ装置に入力記録し、
必要に応じて歯髄組織を損傷しない範囲で抜去乳歯表面を生理食塩水などで洗浄し、
必要に応じて充分に滅菌された保護手段で被包した後に、抜去乳歯を、保持手段を備え真空状態での密封が可能な収納箱の所定位置に保持せしめ、
各種雑菌などの繁殖を阻止して抜去直後の乳歯状態を維持保存するために、収納箱を密封後に空気を抜いた真空状態となし、
その後必要に応じて収納箱内を窒素ガスなどの不活性ガスにより充填・密封し、
収納箱内を所定の低温状態にまで冷却し、
この密封低温状態を適宜な手段により継続させ、
必要に応じて上記各手順の経過および継続状況を乳歯抜去後の時間経過と共に上記コンピュータ装置に入力記録し、
乳歯抜去後所定の時間以内に長期間冷凍保存施設に運搬できるようにしたことを特徴とする再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 Utilizing the technical principles adopted in the two-tank differential pressure dust-free cleaning device in semiconductor wafer manufacturing equipment in the treatment, acquisition, storage, transport, and sterilization of deciduous stem cell tissue for the field of regenerative medicine When transporting to a facility that is frozen and stored for a long period of time,
After confirming that proper oral sterilization has been performed at a facility such as a dental clinic where the extraction procedure is performed, the prescribed milk teeth are extracted,
Input and record basic information including time information about the time elapsed after the extraction procedure for the extracted milk teeth to a computer device that can be connected to the network.
If necessary, remove the surface of the deciduous tooth as long as it does not damage the pulp tissue.
After encapsulating with protective means sufficiently sterilized as necessary, the extracted deciduous teeth are held in place in a storage box that is equipped with holding means and can be sealed in a vacuum state,
In order to prevent the propagation of various germs and maintain and maintain the deciduous teeth state immediately after removal, the storage box is sealed and the air is evacuated,
After that, the storage box is filled and sealed with inert gas such as nitrogen gas as necessary,
Cool the inside of the storage box to a predetermined low temperature state,
Continue this sealed low-temperature state by appropriate means,
If necessary, record the progress and continuation status of each procedure in the computer device with the passage of time after extracting the deciduous teeth,
A system for low-oxygen sterilization, storage, transport, and management of living tissue used for regenerative medicine, characterized in that it can be transported to a cryopreservation facility for a long period of time within a predetermined time after removal of a deciduous tooth. - 乳歯を抜去後、少なくとも２方向以上の側面から挟み込んでの圧接・固定手段により乳歯の先端部分の歯髄細胞が含まれる部分を損傷することなく保護し、
網や籠のような形状とし、取り外し自在なカセット式装置とした台座に必要に応じてピンセットで再度圧接・固定した状態として抜去した乳歯を保持し、
上記台座全体を覆う洗浄用ノズルのような洗浄手段を被せ、
次段階として装置上蓋を閉じて密閉状態とし、
真空ポンプを作動させて装置の内部を−２０Ｋｐａ以下程度の真空状態にし、
その後、ローターを毎分１００回転程度の低速で回転させて、
さらに、必要に応じて生理食塩水、食塩水・オゾン水のような機能水や純水で洗浄し、
さらにその後、低酸素状態で乳歯を保持したままのカセット式装置を取り外して運搬することを特徴とする請求項１又は請求項２に記載の再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 After removing the deciduous teeth, protect them without damaging the portion containing the pulp cells at the tip of the deciduous teeth by pressing and fixing means sandwiched from at least two sides.
Hold the extracted deciduous teeth in a state that has a shape like a net or a bag and is detachable in a cassette-type device that is pressed and fixed again with tweezers as necessary.
Cover the entire pedestal with a cleaning means such as a cleaning nozzle,
As the next step, close the top lid of the device to make it sealed,
Activate the vacuum pump to bring the inside of the device into a vacuum state of about -20 Kpa or less,
Then, rotate the rotor at a low speed of about 100 revolutions per minute,
Furthermore, if necessary, wash with functional water or pure water such as physiological saline, saline / ozone water,
3. Thereafter, the cassette-type device holding the deciduous teeth in a hypoxic state is removed and transported, and the hypoxic sterilization / preservation of biological tissue or the like used for regenerative medicine according to claim 1 or 2 -Transportation and management system. - 抜去した乳歯をアーム式真空ピンセットで自動的・機械的に、低酸素保管庫や−２５℃以下に管理可能な冷却用保管庫に入れて運搬することで、雑菌類を含んだ外部の空気に触れずに運搬することが可能となることを特徴とする請求項１または請求項３何れか一つに記載の再生医療に利用する生体組織の低酸素滅菌・保存・運搬・管理用システム。 Removed deciduous teeth automatically and mechanically with arm-type vacuum tweezers and transported in a low-oxygen storage room or a cooling storage room that can be controlled at -25 ° C or lower, to the outside air containing germs The system for low oxygen sterilization, storage, transportation and management of a living tissue used for regenerative medicine according to any one of claims 1 to 3, wherein the system can be transported without touching.
- ＣＣＤカメラなどの映像記録手段によって歯髄の位置や汚れ状況を確認し、歯髄を採りやすくするためにダイヤモンドドリルのような開穴手段で乳歯の上部に穴を開け、一方で、歯根側から圧縮空気を入れることで押しだされてきた歯髄組織を、ＣＣＤカメラの映像を確認しながらマニピュレータなどにより人手を直接触れさせないで採取するようにしたことを特徴とする請求項１~請求項４の何れか１つに記載の再生医療に利用する生体組織などの低酸素滅菌・保存・運搬・管理用システム。 Check the position and dirt of the pulp with video recording means such as a CCD camera, and make a hole in the upper part of the deciduous tooth with an opening means such as a diamond drill to make it easier to pick the pulp, while compressed air from the root side 5. The dental pulp tissue that has been pushed out by inserting a finger is collected without directly touching a human hand with a manipulator or the like while confirming an image of a CCD camera. A system for low oxygen sterilization, storage, transportation and management of living tissue used for regenerative medicine as described in one.
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