WO2010035530A1

WO2010035530A1 - Measurement result display method and analysis device

Info

Publication number: WO2010035530A1
Application number: PCT/JP2009/055461
Authority: WO
Inventors: 匡羽賀
Original assignee: オリンパス株式会社
Priority date: 2008-09-24
Filing date: 2009-03-19
Publication date: 2010-04-01
Also published as: JP2010078371A

Abstract

Provided is a measurement result display method which displays on a screen, a measurement result obtained by imaging a blood aggregation reaction in each of reaction vessels (wells (W)) of a micro plate (20) for introducing a sample and a reagent separately from each other and performing a blood aggregation reaction. An extraction unit (34) extracts a measurement result corresponding to the inputted extraction item in a measurement unit corresponding to the extraction item. An output unit (32) outputs the extracted measurement result. Thus, it is possible to display a specified measurement result on a single screen.

Description

Measurement result display method and analyzer

The present invention relates to a method for displaying a measurement result of an immunological agglutination reaction of an analyzer.

Conventionally, when analyzing a component of a specimen such as blood or body fluid, a reaction plate that holds a plurality of reaction vessels called a well or a microplate or a reaction card that holds a plurality of reaction vessels is used. In each well of the microplate or each reaction container of the reaction card, a sample containing a substance to be analyzed and a reaction reagent containing a substance that causes an antigen-antibody reaction with the substance to be analyzed are dispensed. Then, after a predetermined time has elapsed from this dispensing, the presence or absence of agglutination reaction occurring in the well or the reaction container is imaged by an imaging means such as a CCD camera, and analysis of the components of the specimen is performed using the image data obtained by this imaging I do.

By the way, the presence or absence of the imaged agglutination reaction can be confirmed on a monitor or the like. In addition, the monitor can confirm the agglutination reaction image of each well of one microplate and can also confirm the reaction image of one well (see, for example, Patent Document 1).

Japanese Patent No. 3206673

However, in the confirmation method shown in Patent Document 1, the measurement results that can be confirmed on one screen are in units of microplates, and the measurement results of a plurality of samples dispensed on the plate are displayed on one screen. The analyst needs to check the measurement results of the sample to be confirmed from the measurement results of the multiple samples, and by displaying multiple samples on one screen, the analyst can display the measurement results. There was a risk of misreading. Further, when the measurement result of one specimen straddles a plurality of plates, the person in charge of analysis has to confirm the measurement result on a plurality of screens. In addition, reaction cards are displayed on a card-by-card basis, and when multiple cards are used for analysis of irregular antibodies, etc., it is necessary to display them separately on the screen, and confirmation of measurement results takes time and effort. .

The present invention has been made in view of the above, and an object thereof is to provide a measurement result display method capable of easily confirming a measurement result.

In order to solve the above-described problems and achieve the object, the present invention uses a substrate having a plurality of reaction vessels for dispensing a specimen and a reagent to cause a blood agglutination reaction, and the blood aggregation generated in each reaction vessel In a measurement result display method for displaying a measurement result obtained by imaging a reaction on a screen, an extraction step for extracting a measurement result corresponding to the input extraction item in a determination unit corresponding to the extraction item is extracted. And outputting the measurement result.

Further, in the measurement result display method according to the present invention, in the above invention, the measurement result includes a captured image captured in units of the reaction container of the extracted item and a reaction determination result read from the captured image. It is characterized by including.

Also, the measurement result display method according to the present invention is characterized in that, in the above-mentioned invention, the extraction step extracts the extraction items input in advance from the items for which analysis has been completed.

In the measurement result display method according to the present invention as set forth in the invention described above, in the output step, when the determination unit corresponding to the extracted item is composed of a plurality of the measurement results, a comprehensive determination of the determination unit Is output.

The analyzer according to the present invention images a blood agglutination reaction occurring in each reaction container using a substrate having a plurality of reaction containers for dispensing a specimen and a reagent and causing a blood agglutination reaction in the above invention. In the analyzer that displays the measurement result obtained on the screen, an input means for inputting an extraction item of the measurement result to be extracted, a storage means for storing the relationship between the measurement result and the determination unit, Referring to the relationship, an extraction unit that extracts a measurement result of the input extraction item in a determination unit corresponding to the extraction item, and an output unit that outputs the extracted measurement result are provided. And

Moreover, in the analysis apparatus according to the present invention, in the above invention, the measurement result includes a captured image captured in units of the reaction container of the extracted item and a reaction determination result read from the captured image. It is characterized by.

In the analysis apparatus according to the present invention as set forth in the invention described above, the extraction means extracts the extraction items input in advance from the items for which analysis has been completed.

In the analysis apparatus according to the present invention as set forth in the invention described above, the output means outputs an overall determination of the determination unit when the determination unit includes a plurality of the measurement results.

In the analysis apparatus according to the present invention, the substrate is a microplate.

Also, the analysis apparatus according to the present invention is characterized in that, in the above invention, the substrate is a reaction card.

According to the present invention, since only the extracted items are displayed on one screen, there is an effect that an accurate measurement result can be easily confirmed.

FIG. 1 is a schematic diagram illustrating the configuration of the analyzer according to the first embodiment. FIG. 2 is a flowchart showing the measurement result display process. FIG. 3 is a diagram illustrating an example of a measurement result display screen displayed on the screen in the measurement result display process. FIG. 4 is a diagram illustrating an example of a pop-up screen displayed when the item selection button is pressed. FIG. 5 is a diagram showing a measurement result display screen in which sample IDs and test items are extracted and displayed based on the measurement results shown in FIG. FIG. 6 is a diagram showing a measurement result display screen displaying measurement results of the ABO blood group. FIG. 7 is a flowchart showing the analysis result display process for displaying the measurement result of the sample ID specified during the analysis process. FIG. 8 is a schematic diagram showing a configuration of an analyzer using a reaction card. FIG. 9 is a perspective view schematically showing a reaction card. FIG. 10 is a diagram showing a measurement result display screen when a reaction card is used.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1,4 Analyzer 2 Measuring mechanism 3,5 Control mechanism 6 Reaction card 10 Plate conveyance lane 11 Sample transfer part

11a Sample container

11b Sample rack 11c Sample reading part 12

Sample dispensing mechanism

12a, 14a, 433, 482

Arm

12b, 12c , 431 Probe 13 Reagent transfer unit 13a Reagent set 13b Reagent reading unit 14 Reagent dispensing mechanism 15 Reaction promoting unit 16 Photometric unit 17 Plate collection unit 20

Microplate

31, 51

Control unit

311, 511

Display control unit

32, 52

Output unit

33 , 53 Analysis unit 34, 54 Extraction unit 35, 55 Transmission /

reception unit

36, 56 Input unit 37, 57 Storage unit 40 Turntable 41 Sample dilution unit 42 Probe cleaning unit 43 Dispensing mechanism 432

Probe holding unit

434, 483 Arm holding unit 44 Reaction card storage 45 Centrifuge 46 Measurement unit 461 CCD camera 47 Reaction card discarding unit 48 Reaction card transfer mechanism 481 Reaction card gripping unit 60 Main unit 61 Reaction vessel 611 Reaction unit 612 Separation unit 62 Seal B1 to B6 Button Cb1 to Cb3, Cb21, Cb22, Cb31 to Cb33 Check Box D1, D2 Captured image database Ib1 Input box T1, T2 Relationship table W Well

Hereinafter, an analyzer according to an embodiment of the present invention will be described with reference to the drawings. Note that the present invention is not limited to the embodiments. In the description of the drawings, the same parts are denoted by the same reference numerals.

FIG. 1 is a schematic diagram showing the configuration of the analyzer according to the present embodiment. As shown in FIG. 1, the analysis apparatus 1 according to the present embodiment dispenses a specimen and a reagent to be analyzed into predetermined wells W (reaction containers) of a microplate 20 and is generated in the wells W. A measurement mechanism 2 that optically measures the reaction, and a control mechanism 3 that controls the entire analyzer 1 including the measurement mechanism 2 and analyzes the measurement result in the measurement mechanism 2 are provided. The analyzer 1 automatically performs immunological analysis of a plurality of specimens by cooperation of these two mechanisms. The microplate 20 is a plate made of a transparent material such as acrylic, and has a number of holes called wells W opened on the surface thereof. The well W is a hole that accommodates a specimen and is formed with an inclined surface, and is arranged in a matrix on the surface of the microplate 20.

The measurement mechanism 2 roughly includes a plate transport lane 10, a sample transfer unit 11, a sample dispensing mechanism 12, a reagent transfer unit 13, a reagent dispensing mechanism 14, a reaction promoting unit 15, a photometric unit 16, and a plate collection unit 17. The control mechanism 3 includes a control unit 31, an output unit 32, an analysis unit 33, an extraction unit 34, a transmission / reception unit 35, an input unit 36, and a storage unit 37. These units included in the measurement mechanism 2 and the control mechanism 3 are electrically connected to the control unit 31.

The plate transport lane 10 transports the microplate 20 to a predetermined position in order to dispense a specimen or reagent to each well W in the microplate 20, promote reaction of the liquid in the well W, and perform photometry. The plate transport lane 10 transports the microplate 20 leftward as indicated by an arrow in FIG. 1, for example, by driving a driving mechanism (not shown) under the control of the control unit 31.

The sample transfer unit 11 includes a plurality of sample racks 11b that hold a plurality of sample containers 11a containing samples and are sequentially transferred in the direction of the arrows in the figure. The specimen contained in the specimen container 11a is obtained by separating the blood collected from the specimen donor by adding an anticoagulant and centrifuging it into plasma as a supernatant and blood cell (red blood cells) particles as a deposit. is there. The sample in the sample container 11a transferred to a predetermined position on the sample transfer unit 11 is dispensed by the sample dispensing mechanism 12 into a predetermined well W of the microplate 20 that is arranged and conveyed on the plate conveyance lane 10. Is done.

A recording medium on which sample information related to the sample stored in the sample container 11a is recorded is attached to the side surface of the sample container 11a. The recording medium displays various encoded information and is optically read. Sample information includes, for example, the name, sex, age, and analysis item of the patient who provided the sample.

A sample reading unit 11c that optically reads this recording medium is provided at a corresponding portion of the sample transfer unit 11. The sample reading unit 11c emits infrared light or visible light to the recording medium, and reads the information on the recording medium by processing the reflected light from the recording medium. In addition, the sample reading unit 11c may perform image capturing processing on the recording medium, decode image information obtained by the image capturing processing, and acquire information on the recording medium. The sample reading unit 11c reads information on the recording medium attached to the sample container 11a when passing in front of the sample reading unit 11c.

The sample dispensing mechanism 12 includes an arm

12a having probes

12b and 12c for aspirating and discharging a sample attached to the tip, and an intake / exhaust mechanism using an unillustrated intake / exhaust syringe or piezoelectric element. The sample dispensing mechanism 12 aspirates the sample with the

probes

12b and 12c from the sample container 11a transferred to the predetermined position on the sample transfer unit 11 described above, moves the arm 12a in the vertical direction in the drawing, A sample is discharged into the well W and dispensed. The probe 12b sucks and discharges serum in the sample container 11a, and the probe 12c sucks and discharges blood cell particles in the sample container 11a.

The reagent transfer unit 13 transfers the reagent set 13a in which the reagent to be dispensed to each well W in the microplate 20 is stored to the reagent aspirating position by the reagent dispensing mechanism 14. The reagent set 13a contains a predetermined amount of required reagents according to various analysis items, and each reagent contained in one set of reagent sets 13a corresponds to a predetermined number of times of dispensing. It may correspond to dispensing. The reagent transfer unit 13 collects the reagent set 13a that has been dispensed a predetermined number of times, and then transfers the reagent set 13a to be dispensed to the reagent suction position.

A recording medium on which reagent information related to each reagent contained in the reagent set 13a is recorded is attached to the side surface of the reagent set 13a. The recording medium displays various encoded information and is optically read. A reagent reading unit 13b that optically reads the recording medium is provided at a corresponding portion of the reagent transfer unit 13. The reagent reading unit 13b emits infrared light or visible light to the recording medium, and reads the information on the recording medium by processing the reflected light from the recording medium. Further, the reagent reading unit 13b may acquire the information on the recording medium by performing an imaging process on the recording medium and decoding the image information obtained by the imaging process.

The reagent dispensing mechanism 14 includes an arm 14a with a probe for aspirating and discharging the reagent attached to the tip. The arm 14a freely moves up and down in the vertical direction and rotates around the vertical line passing through its base end as a central axis. The reagent dispensing mechanism 14 includes a suction / discharge mechanism using a suction / discharge syringe or a piezoelectric element (not shown). The reagent dispensing mechanism 14 sucks the reagent in the reagent set 13a that has been moved to a predetermined position on the reagent transfer unit 13 with the corresponding probes, and rotates the arm 14a counterclockwise in the figure to thereby move the plate conveyance lane 10. Dispensing is performed by discharging each reagent corresponding to each well W of the microplate 20 conveyed to a predetermined position above.

The reaction promoting unit 15 promotes the reaction of the sample and the reagent dispensed on the microplate 20 to cause the antigen-antibody reaction to form an aggregation pattern on the bottom surface of each well W of the microplate 20. For example, the reaction promoting unit 15 vibrates the microplate 20 and stirs the specimen and the reagent in the well W. In addition, the reaction promoting unit 15 keeps the microplate 20 for a predetermined time corresponding to the content of the analysis method, for example, and promotes natural sedimentation of blood cell particles. Further, the reaction promoting unit 15 manipulates the fine particles present in the well W by applying a predetermined magnetic field, for example.

The photometric unit 16 photometrically detects the aggregation pattern formed in the reaction promoting unit 15. The photometry unit 16 is configured by, for example, a CCD camera, images each well W of the microplate 20 from above, and outputs image information obtained by imaging the aggregation pattern formed in each well W. The photometry unit 16 includes a light emitting unit that irradiates each well W of the microplate 20 with predetermined light and a light receiving unit that receives light generated in the test solution in each well W, and light generated in the test solution. May be output as a photometric result.

The plate collection unit 17 collects the microplate 20 that has been subjected to photometric processing by the photometric unit 16. The collected microplate 20 is washed by a washing unit (not shown) by sucking and discharging the mixed solution in each well W, and injecting and sucking the washing solution. The washed microplate 20 is reused. Depending on the contents of the inspection, the microplate 20 may be discarded after one measurement.

Next, the control mechanism 3 will be described. The control unit 31 is configured using a CPU or the like, and controls processing and operation of each unit of the analyzer 1. The control unit 31 performs predetermined input / output control on information input / output to / from each of these components, and performs predetermined information processing on this information. In addition, the control unit 31 includes a display control unit 311. The display control unit 311 controls display of the measurement result extracted by the extraction unit 34.

The output unit 32 is configured using a display, a printer, a speaker, and the like, and outputs various information including the analysis information generated by the analysis unit 33. Further, the image information extracted by the extraction unit 34 is output to the screen.

The analyzing unit 33 analyzes the antigen-antibody reaction based on the photometric result measured by the photometric unit 16. In addition, when the photometry unit 16 outputs image information, the analysis unit 33 processes the image information output by the photometry unit 16 and acquires a photometric value corresponding to the luminance of the specimen. The analysis unit 33 uses SPC (brightness of the edge of the image of the central portion), P (brightness of the peripheral portion), C (brightness of the central portion), which is used to determine whether the agglutination reaction is negative or positive. Parameters such as LIA (center area image area) are calculated and compared with the stored threshold values of the parameters of SPC, P, C, and LIA. It should be noted that the SPC, P, and C parameters are obtained from 0 to 99, and the LIA parameter is obtained from 0 to 999. Comparing the calculated parameter value with the parameter threshold value, each test item can be determined as + (positive),-(negative), or unknown (between positive and negative and neither can be determined) is there.

When the extraction item is input from the input unit 36 to the control unit 31, the extraction unit 34 extracts the extraction item from the captured image database D1 while referring to the relation table T1. The extracted captured image is output to the output unit 32.

The transmission / reception unit 35 has a function as an interface for performing transmission / reception of information according to a predetermined format via a communication network (not shown). The input unit 36 is configured by using a keyboard, a mouse, a microphone, and the like, and acquires various information necessary for analyzing the sample, instruction information for analysis operation, and the like from the outside. In addition, an extraction item to be displayed on the screen is output to the control unit 31.

The storage unit 37 is configured using a hard disk that magnetically stores information and a memory that electrically loads various programs related to the process from the hard disk when the analyzer 1 executes the process. . The storage unit 37 may include an auxiliary storage device that can read information stored in a storage medium such as a CD-ROM, a DVD-ROM, or a PC card. The storage unit 37 includes a relationship table T1 and a captured image database D1. The relationship table T1 stores the relationship of each reagent with respect to each inspection item. For example, when the test item is an ABO blood group, anti-A antibody, anti-B antibody, A blood cell, and B blood cell are stored as related reagents. When extracting the input extraction item, the extraction unit 34 refers to the relationship table T1 and extracts information corresponding to the item. The captured image database D1 stores negative or positive determination results, specimen IDs, and image information associated with test items, which are captured by the photometric unit 16.

In the analyzer 1 configured as described above, the sample dispensing mechanism 12 dispenses the sample in the sample container 11a with respect to the plurality of microplates 20 that are sequentially conveyed, and the reagent dispensing mechanism 14 uses the reagent set. After dispensing each reagent in 13a, the photometry unit 16 measures the luminance of the sample in a state where the sample and the reagent are reacted, and the analysis unit 33 analyzes the measurement result, whereby the antigen-antibody reaction of the sample is performed. Analysis is automatically performed.

Here, the measurement result display process for displaying the measurement result after the end of the analysis process will be described with reference to FIG. FIG. 2 is a flowchart showing the measurement result display process.

First, the control unit 31 confirms whether or not the sample ID for displaying the measurement result is input to the input unit 36 (step S102). When the sample ID is input to the input unit 36 (step S102: Yes), the control unit 31 instructs the extraction unit 34 to extract the measurement result of the input sample ID (step S104). Thereafter, the control unit 31 confirms whether or not a test item to be displayed has been input for the measurement result of the extracted sample ID (step S106). When the inspection item is input to the input unit 36 (step S106: Yes), the control unit 31 instructs the extraction unit 34 to extract the specified inspection item from the extracted measurement result (step S108). . When the extraction of the designated sample ID and test item is completed, the control unit 31 instructs the display control unit 311 to display the extracted measurement result (step S110).

Here, when there is no sample ID input in step S102 (step S102: No), the control unit 31 proceeds to step S110 and instructs the display control unit 311 to display the measurement result in units of microplates. If no test item is input in step S106 (step S106: No), the control unit 31 proceeds to step S110 and instructs the display control unit 311 to display all measurement results of the specified sample ID. To display the measurement result. Two or more sample IDs may be input.

FIG. 3 is a diagram showing an example of a measurement result display screen displayed on the screen in the measurement result display process. When the analysis is completed, the measurement result display screen W1 is displayed on the screen. When the sample ID or the examination item is not specified, the measurement result output on the screen is displayed in units of microplates. Here, the column of the table indicates each reagent, and the row indicates the measurement result of the same specimen ID. Moreover, the negative or positive result of the agglutination reaction is displayed on the right side of each image, and the corresponding comprehensive determination result is displayed on the right side of the table. The ABO blood group and the Rho (D) blood group are comprehensively determined by combinations of the reagent determination results shown in Tables 1 and 2. Table 1 shows the negative (-) and positive (+) combinations of each reagent of ABO blood group, and Table 2 shows the negative and positive combinations of Rho (D) blood group. The overall judgment is determined by comprehensively combining negative and positive results. 3 is based on the relationship shown in Table 1, anti-A antibody (-A), anti-B antibody (-B), A blood cell (Acell), B blood cell (Bcell) The ABO blood group determination result was determined by comprehensively determining the agglutination reaction against the anti-D antibody (−D) and the control (Ref) based on the relationship shown in Table 2. The Rho (D) blood type determination result determined in this way is output. When each captured image is selected, the selected image is displayed in a pop-up, and an enlarged captured image can be displayed, and each agglutination reaction can be confirmed.

In the input box Ib1, the sample ID to be extracted can be input, and the measurement result of the sample ID can be displayed by inputting the sample ID and pressing the display button B1. If no sample ID is input, the sample ID corresponding to the image in the selection unit P selected on the screen is displayed in the input box Ib1. Further, the help button B3 is pressed according to the situation to confirm the operation, and when the work is ended, the end button B4 is pressed.

Here, when the displayed inspection item is designated, the item can be selected by pressing the item selection button B2. FIG. 4 is a diagram illustrating an example of a pop-up screen displayed when the item selection button B2 is pressed. In the item selection screen W2 displayed as a pop-up screen, a check box is provided for each item, the check box of the item to be displayed is selected, and the OK button B5 is pressed, and the measurement result of the item is displayed on the screen shown in FIG. Can be displayed. When displaying the measurement results of all items, select the check box Cb1 and press the OK button B5. In addition, when displaying the blood group measurement result, it is possible to display by selecting the check box Cb2, and when displaying the ABO blood group measurement result, the check box Cb21 is selected and the Rho (D) blood is displayed. When displaying the measurement result of the mold, the check box Cb22 is selected. In addition, when displaying the measurement result of the irregular antibody screening, the check box Cb3 is selected and displayed. Here, when the check boxes Cb31 to 33 are selected, the measurement results of each measurement method (antiglobulin method, enzyme method, and saline solution method) can be displayed. When canceling the item selection, the cancel button B6 is pressed.

5 and 6 are diagrams showing measurement result display screens in which sample IDs and test items are extracted and displayed based on the measurement results shown in FIG. FIG. 5 is a measurement result display screen W3 in which the measurement result of one specimen ID in FIG. 3 is extracted and displayed. The measurement result display screen W3 displays the measurement result of the designated sample ID by inputting the sample ID into the input box Ib1 and pressing the display button B1. In addition, the determination result is displayed on the right side of the screen in accordance with the displayed measurement result.

Here, when the item selection button B2 is pressed on the screen shown in FIG. 5 and an item is selected, the selected measurement result is displayed. FIG. 6 is a diagram showing a measurement result display screen W4 in which the check box Cb21 in FIG. 4 is selected and the measurement result of the ABO blood group is displayed. When the check box Cb21 is selected in FIG. 4, the extraction unit 34 extracts the relationship of the image information corresponding to the extraction item from the captured image database D1 with reference to the relationship table T1. In the case of the ABO blood group, anti-A antibody (-A), anti-B antibody (-B), A blood cell (Acell), and B blood cell (Bcell) are required to determine the test item. Image information corresponding to the above four items is extracted and output. The determination result is displayed as a comprehensive determination.

Note that the measurement result display process can also display the measurement result of the sample ID specified during the analysis process. FIG. 7 is a flowchart showing the analysis result display process for displaying the measurement result of the sample ID specified during the analysis process.

First, after performing an analysis process (step S202), the control unit 31 receives a measurement result from the analysis unit 33, and confirms whether a sample ID for displaying the measurement result is specified on the input unit 36 (step S204). ). When the sample ID to be displayed is designated (step S204: Yes), the control unit 31 instructs the extraction unit 34 to extract the measurement result of the designated sample ID (step S206). When the measurement result of the sample ID is extracted, the control unit 31 confirms whether or not there is a related examination instruction in the measurement result of the sample ID (step S208). Here, when there is an instruction for the related test for the sample ID (step S208: Yes), the control unit 31 waits for the determination process from the analysis unit 33 and causes the extraction unit 34 to extract the related test result of the sample ID. (Step S210). Confirmation is repeated until the extraction is completed (step S210: No), and when the extraction of the measurement result of the related examination is completed (step S210: Yes), the control unit 31 causes the display control unit 311 to display the extracted measurement result. An instruction is issued (step S212). Thereafter, the control unit 31 confirms whether or not there is an instruction to analyze the next sample. If there is an instruction to analyze the next sample (step S214: Yes), the control unit 31 proceeds to step S202 to perform analysis processing. When there is no next analysis instruction (step S214: No), the control unit 31 ends the work.

In step S204, when the sample ID is not designated (step S204: No), the control unit 31 moves to step S212 and instructs the display control unit 311 to display the measurement result in units of microplates. . In step S208, when there is no related examination instruction for the sample ID (step S208: No), the control unit 31 proceeds to step S212 and displays the measurement result extracted in step S206 on the screen. An instruction is issued to the control unit 311. Here, the related test refers to the result of the irregular antibody identification test in the case of being positive by the irregular antibody screening. In addition, you may display the result of the reexamination in an analysis process.

Further, the above-described measurement result display method can be applied to a reaction card having a plurality of reaction containers. FIG. 8 is a schematic diagram showing a configuration of the analyzer 4 using the reaction card 6, and FIG. 9 is a perspective view schematically showing the reaction card 6.

The analyzer 4 includes a turntable 40 that holds various containers such as a specimen container, a reagent container, and a diluent container, a dispensing mechanism 43 that dispenses a specimen and a reagent using a thin tubular probe, and a specimen and a reagent. A reaction card storage unit 44 for storing a plurality of unused reaction cards 6 to be dispensed, a centrifuge 45 that can mount the reaction card 6 and applies centrifugal force to the mounted reaction card 6, and a centrifugal operation of the centrifuge 45 A measurement unit 46 that measures the reaction image generated by the measurement unit, a reaction card discard unit 47 that discards the reaction card 6 that has been measured by the measurement unit 46, a reaction card transfer mechanism 48 that transfers the reaction card 6, and the analyzer 4 A control mechanism 5 that performs overall control and analyzes the measurement result is provided.

The dispensing mechanism 43 includes a probe cleaning unit 42 that cleans the probe, and a sample dilution unit 41 that dilutes the sample by mixing the sample and the diluent. In the analysis, when it is necessary to dilute the sample, the sample is diluted by the sample diluting unit 41.

The dispensing mechanism 43 includes a tubular probe 431 that sucks and discharges a specimen, a reagent, and a diluent, a probe holder 432 that holds the probe 431, and a probe holder 432 in the y-axis direction and the z-axis in FIG. The arm 433 is movably supported in the direction, and the arm holding portion 434 is movably supported in the x-axis direction of FIG. The probe 431 sucks the specimen, reagent, and diluent contained in the specimen container, reagent container, and diluent container on the turntable 40, and is diluted with the reaction card 6 held by the centrifuge 45. Dispense the reagent. The probe 431 can reach the specimen dilution unit 41 and the probe cleaning unit 42 by the operations of the probe holding unit 432 and the arm 433. In addition, it is good also as a structure which each dispenses a test substance, a reagent, and a diluent by a separate dispensing mechanism.

The centrifuge 45 has a rotating head that holds a plurality of reaction cards 6 with a circular surface and applies centrifugal force to each reaction card 6 by rotation.

The measuring unit 46 has a CCD camera 461, captures the reaction image of the reaction card 6 transferred from the centrifuge 45 by the reaction card transfer mechanism 48, and transmits it to the analysis unit 53.

The reaction card transfer mechanism 48 includes a reaction card gripping portion 481 that grips the reaction card 6, an arm 482 that supports the reaction card gripping portion 481 movably in the x-axis direction and the z-axis direction of FIG. And an arm holding portion 483 that supports the end portion so as to be movable in the y-axis direction of FIG. The reaction card transfer mechanism 48 transfers the reaction card 6 accommodated in the reaction card storage unit 44 to the centrifuge 45, transfers the reaction card 6 that has been centrifuged in the centrifuge 45 to the measurement unit 46, and the measurement unit 46. It has a function of transferring the reaction card 6 whose measurement has been completed to the reaction card discarding unit 47. In addition, the arm 482 is arrange | positioned in the position which does not interfere with the arm 433 of the dispensing mechanism 43 in the z-axis direction of FIG.

Next, the control mechanism 5 will be described. The control unit 51 is configured using a CPU or the like, and controls processing and operation of each unit of the analyzer 4. The control unit 51 performs predetermined input / output control on information input / output to / from each of these components, and performs predetermined information processing on this information. In addition, the control unit 51 includes a display control unit 511. The display control unit 511 controls the display of the measurement result extracted by the extraction unit 54.

The output unit 52 is configured using a display, a printer, a speaker, and the like, and outputs various information including the analysis information generated by the analysis unit 53. Further, the image information extracted by the extraction unit 54 is output to the screen.

The analysis unit 53 analyzes the antigen-antibody reaction based on the photometric result measured by the measurement unit 46. When the measurement unit 46 outputs image information, the analysis unit 53 processes the image information output by the measurement unit 46 and acquires a photometric value corresponding to the luminance of the specimen. In addition, the analysis unit 53 compares the parameters used for determining whether the agglutination reaction is negative or positive with a threshold value, and determines whether 4+ to 1+ (positive), − (negative), unknown (between positive and negative) for each test item. In the case where neither can be determined).

When the extraction unit 54 receives an input of an extraction item from the input unit 56, the extraction unit 54 extracts the extraction item from the captured image database D2 while referring to the relationship table T2. The extracted captured image is output to the output unit 52.

The transmission / reception unit 55 has a function as an interface for performing transmission / reception of information according to a predetermined format via a communication network (not shown). The input unit 56 is configured by using a keyboard, a mouse, a microphone, and the like, and acquires various information necessary for analyzing the specimen, instruction information for analysis operation, and the like from the outside. In addition, the extraction items to be displayed on the screen are output to the control unit 51.

The storage unit 57 includes a hard disk that magnetically stores information and a memory that loads various programs related to the processing from the hard disk and electrically stores them when the analyzer 4 executes the processing. . The storage unit 57 may include an auxiliary storage device that can read information stored in a storage medium such as a CD-ROM, a DVD-ROM, or a PC card. The storage unit 57 includes a relationship table T2 and a captured image database D2. The relationship table T2 stores the relationship of each reagent with respect to each inspection item. For example, when the test item is irregular antibody screening, each measurement result by the antiglobulin method, the enzyme method, and the saline method is stored as a related test item. When extracting the input extraction item, the extraction unit 54 refers to the relationship table T2 and extracts information corresponding to the item. The captured image database D2 stores image information associated with negative or positive determination results, specimen IDs, and test items in the captured images of the separation units 612 of the reaction card 6 illustrated in FIG. .

In the analyzer 4 configured as described above, the dispensing mechanism 43 dispenses the specimen container held on the turntable 40, the specimen in the reagent container, and the reagent with respect to the plurality of reaction cards 6 that are sequentially transported. After the injection, the centrifuge 45 applies a centrifugal force to the reaction card 6 to move the reaction product, and the measurement unit 46 performs imaging measurement of the sample in a state where the sample and the reagent are reacted, and analyzes the measurement result. Analysis by the unit 53 automatically performs antigen-antibody reaction analysis of the specimen.

The reaction card 6 shown in FIG. 9 includes a card-shaped main body 60, a reaction container 61 having an opening in the upper flat surface of the main body 60, and a main body 60 that is attached to a bar code or lot number for identification. An effective date and the like are printed, and a seal 62 having a column for sample information and reaction results is provided. The reaction vessel 61 has a substantially cylindrical shape and is provided in communication with a reaction unit 611 for reacting a specimen and a reagent, and a bottom of the reaction unit 611, and a reaction for determining the presence or absence of aggregation according to the result of the reaction. A separation unit 612 holds gel or glass beads or the like as a carrier for generating an image and holds a reaction solution obtained by antigen-antibody reaction between a specimen and a reagent, which is centrifuged by a centrifuge 45. The reaction card 6 is formed using a translucent resin. The aggregated image in the separation unit 612 is confirmed by imaging, and each separation unit 612 captured from the side surface of the reaction card 6 is displayed on the screen.

Here, the measurement result display using the reaction card 6 will be described with reference to FIG. FIG. 10 is a diagram showing a measurement result display screen W5 when the reaction card 6 is used.

As shown in FIG. 10, each separation unit 612 of the captured reaction card 6 is displayed on the measurement result display screen W5. Here, when irregular antibody screening is performed using the reaction card 6, the analysis is performed using a plurality of reaction cards depending on the measurement method (antiglobulin method, enzyme method, saline solution method). In particular, in the anti-globulin method, a reaction card filled with an anti-human globulin antibody together with a carrier is used for the separation unit 612 in order to react with an anti-human globulin antibody after reacting a specimen and a blood cell reagent. The extraction unit 54 extracts the image information designated on the item selection screen W2 shown in FIG. 4 from the captured image database D2 stored in the storage unit 57, so that the measurement results with different reaction cards can be obtained. Can be displayed on the screen. In addition, each measurement method is displayed on the upper part of the image, and the measurement result is confirmed by comparing the measurement results analyzed by each measurement method on one screen. Similar to FIGS. 3, 5, and 6, images can be arbitrarily extracted by inputting the sample ID in the input box Ib1 and pressing the display button B1, or by using the item selection button B2, and the reaction card is different. Even in this case, an image can be displayed on one screen.

In this embodiment, it is possible to display only specified items by inputting or selecting items to be displayed, and even if the measurement results are different microplates and reaction cards, the measurement results can be displayed on one screen. Can be displayed.

As described above, the measurement result display method and analysis apparatus according to the present invention are useful for confirming measurement results including captured images, and are particularly suitable for analysis apparatuses that display a plurality of analysis results.

Claims

Measurement result display that displays the measurement results obtained by imaging the blood agglutination reaction that occurred in each reaction container on the screen using a substrate that has multiple reaction containers that dispense blood samples and reagents to cause a blood agglutination reaction In the method
An extraction step for extracting a measurement result corresponding to the input extraction item in a determination unit corresponding to the extraction item;
An output step of outputting the extracted measurement result;
A measurement result display method comprising:
The measurement result display method according to claim 1, wherein the measurement result includes a captured image captured in units of the reaction container of the extracted item and a reaction determination result read from the captured image.
The extraction step includes
3. The measurement result display method according to claim 1 or 2, wherein the extraction items input in advance are extracted in order from an item for which analysis has been completed.
The output step includes
The measurement according to any one of claims 1 to 3, wherein when the determination unit corresponding to the extracted item includes a plurality of the measurement results, a comprehensive determination of the determination unit is output. Result display method.
In an analyzer that displays a measurement result obtained by imaging a blood agglutination reaction occurring in each reaction vessel on a screen using a substrate having a plurality of reaction vessels for dispensing a specimen and a reagent to cause a blood agglutination reaction ,
An input means for inputting an extraction item of the measurement result to be extracted;
Storage means for storing the relationship between the measurement result and the determination unit;
Referring to the relationship of the storage means, extracting means for extracting the measurement result of the input extraction item in a determination unit corresponding to the extraction item;
Output means for outputting the extracted measurement results;
An analyzer characterized by comprising:
The analyzer according to claim 5, wherein the measurement result includes a captured image captured in units of the reaction container of the extracted item and a reaction determination result read from the captured image.
The analysis apparatus according to claim 5 or 6, wherein the extraction means extracts the extraction items input in advance from the items that have been analyzed.
The analyzer according to any one of claims 5 to 7, wherein the output means outputs an overall determination of the determination unit when the determination unit is composed of a plurality of the measurement results.
The analyzer according to any one of claims 5 to 8, wherein the substrate is a microplate.
The analyzer according to any one of claims 5 to 8, wherein the substrate is a reaction card.