WO2010017230A3 - Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol - Google Patents

Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol Download PDF

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Publication number
WO2010017230A3
WO2010017230A3 PCT/US2009/052748 US2009052748W WO2010017230A3 WO 2010017230 A3 WO2010017230 A3 WO 2010017230A3 US 2009052748 W US2009052748 W US 2009052748W WO 2010017230 A3 WO2010017230 A3 WO 2010017230A3
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WO
WIPO (PCT)
Prior art keywords
production
bio
methods
isobutanol
butanol
Prior art date
Application number
PCT/US2009/052748
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English (en)
Other versions
WO2010017230A2 (fr
Inventor
Michael D. Lynch
Original Assignee
The Regents Of The University Of Colorado
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of Colorado filed Critical The Regents Of The University Of Colorado
Priority to US13/057,359 priority Critical patent/US20110281314A1/en
Publication of WO2010017230A2 publication Critical patent/WO2010017230A2/fr
Publication of WO2010017230A3 publication Critical patent/WO2010017230A3/fr
Priority to US14/106,409 priority patent/US20140170731A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne généralement des procédés, des compositions, des systèmes et des utilisations pour permettre la bioproduction de ou l'augmentation de la bioproduction de molécules d'alcool par des micro-organismes. Certains modes de réalisation concernent des compositions et des procédés permettant ou augmentant la bioproduction de molécules d'alcool à 4 carbones par des bactéries. Dans certains modes de réalisation, des compositions et des procédés concernent l'ajout d'isobutyryl-CoA isomérase à une culture de micro-organismes pour permettre ou augmenter la bioproduction d'alcools à quatre carbones. La présente invention concerne en outre des variantes des voies de biosynthèse pour la bioproduction microbienne de butanol et/ou d'isobutanol.
PCT/US2009/052748 2008-08-04 2009-08-04 Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol WO2010017230A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/057,359 US20110281314A1 (en) 2008-08-04 2009-08-04 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol
US14/106,409 US20140170731A1 (en) 2008-08-04 2013-12-13 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8598608P 2008-08-04 2008-08-04
US61/085,986 2008-08-04

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/057,359 A-371-Of-International US20110281314A1 (en) 2008-08-04 2009-08-04 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol
US14/106,409 Continuation US20140170731A1 (en) 2008-08-04 2013-12-13 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol

Publications (2)

Publication Number Publication Date
WO2010017230A2 WO2010017230A2 (fr) 2010-02-11
WO2010017230A3 true WO2010017230A3 (fr) 2010-07-22

Family

ID=41664175

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/052748 WO2010017230A2 (fr) 2008-08-04 2009-08-04 Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol

Country Status (2)

Country Link
US (2) US20110281314A1 (fr)
WO (1) WO2010017230A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130149755A1 (en) 2008-11-06 2013-06-13 Kiverdi ,Inc. Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds
EP2464736A4 (fr) 2009-08-12 2013-07-31 Gevo Inc Localisation de la voie de l'isobutanol cytosolique pour la production d'isobutanol
CA2784903A1 (fr) * 2009-12-29 2011-07-28 Butamax(Tm) Advanced Biofuels Llc Alcool deshydrogenases (adh) utiles pour la production par fermentation d'alcools alkyliques inferieurs
JP6061862B2 (ja) 2010-11-22 2017-01-18 カーギル，インコーポレイティド ３‐ヒドロキシプロピオン酸生産用の組成物及び方法
WO2012109534A2 (fr) * 2011-02-11 2012-08-16 Regents Of The University Of Minnesota Cellules et procédés de production d'acide isobutyrique
ES2728279T3 (es) 2011-11-03 2019-10-23 Easel Biotechnologies Llc Producción microbiana de n-butiraldehído
WO2013090769A2 (fr) 2011-12-14 2013-06-20 Kiverdi, Inc. Procédé et appareil pour la croissance de cultures microbiennes qui nécessitent des donneurs d'électrons, accepteurs d'électrons, sources de carbone ou autres nutriments gazeux
SG11201501013PA (en) 2012-08-10 2015-04-29 Opx Biotechnologies Inc Microorganisms and methods for the production of fatty acids and fatty acid derived products
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
EP2970086B1 (fr) 2013-03-15 2018-11-07 Cargill, Incorporated Récupération d'acide 3-hydroxypropionique
BR112016001026A2 (pt) 2013-07-19 2017-10-24 Cargill Inc organismo geneticamente modificado
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP2993228B1 (fr) 2014-09-02 2019-10-09 Cargill, Incorporated Production d'esters d'acides gras
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040152159A1 (en) * 2002-11-06 2004-08-05 Causey Thomas B. Materials and methods for the efficient production of acetate and other products
US20070092957A1 (en) * 2005-10-26 2007-04-26 Donaldson Gail K Fermentive production of four carbon alcohols
WO2008072920A1 (fr) * 2006-12-15 2008-06-19 Biofuelchem Co., Ltd. Procédé de préparation de butanol par le biais de bactéries utilisant le butyryle-coa comme intermédiaire

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103540559A (zh) * 2007-02-09 2014-01-29 加利福尼亚大学董事会 利用重组微生物生产生物燃料

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040152159A1 (en) * 2002-11-06 2004-08-05 Causey Thomas B. Materials and methods for the efficient production of acetate and other products
US20070092957A1 (en) * 2005-10-26 2007-04-26 Donaldson Gail K Fermentive production of four carbon alcohols
WO2008072920A1 (fr) * 2006-12-15 2008-06-19 Biofuelchem Co., Ltd. Procédé de préparation de butanol par le biais de bactéries utilisant le butyryle-coa comme intermédiaire

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAUREN SALLUS JENKINS ET AL.: "Genetic and Molecular Characterization of the Genes Involved in Short-chain Fatty Acid Degradation in Escherichia coli: the ato System.", JOURNAL OF BACTERIOLOGY, vol. 169, no. 1, January 1987 (1987-01-01), pages 42 - 52 *
MASAYUKI INUI ET AL.: "Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli.", APPL. MICROBIOL. BIOTECHNOL, vol. 77, 2008, pages 1305 - 1316 *

Also Published As

Publication number Publication date
US20140170731A1 (en) 2014-06-19
WO2010017230A2 (fr) 2010-02-11
US20110281314A1 (en) 2011-11-17

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