WO2010017230A3 - Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol - Google Patents

Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol Download PDF

Info

Publication number
WO2010017230A3
WO2010017230A3 PCT/US2009/052748 US2009052748W WO2010017230A3 WO 2010017230 A3 WO2010017230 A3 WO 2010017230A3 US 2009052748 W US2009052748 W US 2009052748W WO 2010017230 A3 WO2010017230 A3 WO 2010017230A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
bio
methods
isobutanol
butanol
Prior art date
Application number
PCT/US2009/052748
Other languages
English (en)
Other versions
WO2010017230A2 (fr
Inventor
Michael D. Lynch
Original Assignee
The Regents Of The University Of Colorado
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of Colorado filed Critical The Regents Of The University Of Colorado
Priority to US13/057,359 priority Critical patent/US20110281314A1/en
Publication of WO2010017230A2 publication Critical patent/WO2010017230A2/fr
Publication of WO2010017230A3 publication Critical patent/WO2010017230A3/fr
Priority to US14/106,409 priority patent/US20140170731A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne généralement des procédés, des compositions, des systèmes et des utilisations pour permettre la bioproduction de ou l'augmentation de la bioproduction de molécules d'alcool par des micro-organismes. Certains modes de réalisation concernent des compositions et des procédés permettant ou augmentant la bioproduction de molécules d'alcool à 4 carbones par des bactéries. Dans certains modes de réalisation, des compositions et des procédés concernent l'ajout d'isobutyryl-CoA isomérase à une culture de micro-organismes pour permettre ou augmenter la bioproduction d'alcools à quatre carbones. La présente invention concerne en outre des variantes des voies de biosynthèse pour la bioproduction microbienne de butanol et/ou d'isobutanol.
PCT/US2009/052748 2008-08-04 2009-08-04 Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol WO2010017230A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/057,359 US20110281314A1 (en) 2008-08-04 2009-08-04 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol
US14/106,409 US20140170731A1 (en) 2008-08-04 2013-12-13 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8598608P 2008-08-04 2008-08-04
US61/085,986 2008-08-04

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/057,359 A-371-Of-International US20110281314A1 (en) 2008-08-04 2009-08-04 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol
US14/106,409 Continuation US20140170731A1 (en) 2008-08-04 2013-12-13 Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol

Publications (2)

Publication Number Publication Date
WO2010017230A2 WO2010017230A2 (fr) 2010-02-11
WO2010017230A3 true WO2010017230A3 (fr) 2010-07-22

Family

ID=41664175

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/052748 WO2010017230A2 (fr) 2008-08-04 2009-08-04 Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol

Country Status (2)

Country Link
US (2) US20110281314A1 (fr)
WO (1) WO2010017230A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130149755A1 (en) 2008-11-06 2013-06-13 Kiverdi ,Inc. Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds
CA2770842A1 (fr) 2009-08-12 2011-02-17 Gevo, Inc. Localisation de la voie de l'isobutanol cytosolique pour la production d'isobutanol
KR20120115349A (ko) 2009-12-29 2012-10-17 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 저급 알킬 알코올의 발효 생성에 유용한 알코올 탈수소효소(ａｄｈ)
MX2013005654A (es) 2010-11-22 2013-07-17 Novozymes Inc Composiciones y metodos para la produccion de acido 3-hidroxipropionico.
KR20140061303A (ko) * 2011-02-11 2014-05-21 리전츠 오브 더 유니버스티 오브 미네소타 이소부티르산을 생산하기 위한 세포 및 방법
CN104254609B (zh) 2011-11-03 2017-09-19 伊塞尔生物技术有限责任公司 正丁醛的微生物生产
US9157058B2 (en) 2011-12-14 2015-10-13 Kiverdi, Inc. Method and apparatus for growing microbial cultures that require gaseous electron donors, electron acceptors, carbon sources, or other nutrients
CN104718282A (zh) 2012-08-10 2015-06-17 Opx生物工艺学公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
WO2014146026A1 (fr) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction de substances chimiques
BR112015023474B1 (pt) 2013-03-15 2020-10-06 Cargill, Incorporated Método para recuperar uma composição enriquecida em ácido 3-hidroxipropiônico
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
EP2993228B1 (fr) 2014-09-02 2019-10-09 Cargill, Incorporated Production d'esters d'acides gras
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
WO2018144701A2 (fr) 2017-02-02 2018-08-09 Cargill Incorporated Cellules génétiquement modifiées produisant des dérivés d'acides gras en c6-c10

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040152159A1 (en) * 2002-11-06 2004-08-05 Causey Thomas B. Materials and methods for the efficient production of acetate and other products
US20070092957A1 (en) * 2005-10-26 2007-04-26 Donaldson Gail K Fermentive production of four carbon alcohols
WO2008072920A1 (fr) * 2006-12-15 2008-06-19 Biofuelchem Co., Ltd. Procédé de préparation de butanol par le biais de bactéries utilisant le butyryle-coa comme intermédiaire

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011510611A (ja) * 2007-02-09 2011-04-07 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 組換え微生物によるバイオ燃料の生成

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040152159A1 (en) * 2002-11-06 2004-08-05 Causey Thomas B. Materials and methods for the efficient production of acetate and other products
US20070092957A1 (en) * 2005-10-26 2007-04-26 Donaldson Gail K Fermentive production of four carbon alcohols
WO2008072920A1 (fr) * 2006-12-15 2008-06-19 Biofuelchem Co., Ltd. Procédé de préparation de butanol par le biais de bactéries utilisant le butyryle-coa comme intermédiaire

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAUREN SALLUS JENKINS ET AL.: "Genetic and Molecular Characterization of the Genes Involved in Short-chain Fatty Acid Degradation in Escherichia coli: the ato System.", JOURNAL OF BACTERIOLOGY, vol. 169, no. 1, January 1987 (1987-01-01), pages 42 - 52 *
MASAYUKI INUI ET AL.: "Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli.", APPL. MICROBIOL. BIOTECHNOL, vol. 77, 2008, pages 1305 - 1316 *

Also Published As

Publication number Publication date
WO2010017230A2 (fr) 2010-02-11
US20110281314A1 (en) 2011-11-17
US20140170731A1 (en) 2014-06-19

Similar Documents

Publication Publication Date Title
WO2010017230A3 (fr) Procédés, systèmes et compositions associés à la bioproduction microbienne de butanol et/ou d'isobutanol
WO2008089102A3 (fr) Compositions et procédés destinés à améliorer la tolérance de production de produits chimiques organiques à partir de microorganismes
WO2010014632A3 (fr) Procédés et compositions permettant d'améliorer la production de certains produits dans des micro-organismes
WO2011127409A3 (fr) Procédés et compositions associés à des enzymes biosynthétiques d'alcools gras
WO2009049274A8 (fr) Micro-organisme modifié pour produire de l'isopropanol
WO2012115527A3 (fr) Micro-organismes recombinants et utilisations de ceux-ci
UA103364C2 (ru) Способ ферментативного производства четырехатомных спиртов
WO2010014631A3 (fr) Procédés et compositions permettant d'améliorer la production de certains produits dans des micro-organismes
WO2007130518A3 (fr) Production par fermentation d'alcools à quatre atomes de carbone
EP2749652A3 (fr) Procédé de production d'une substance cible par fermentation
WO2009076480A3 (fr) Méthylbutanol en tant que biocarburant amélioré
WO2012177619A3 (fr) Microorganismes pour la production de 1,3-butanediol et procédés associés à ceux-ci
MX2013008988A (es) Microorganismo y metodos para la biosintesis de butadieno.
WO2011008564A3 (fr) Compositions et procédés pour la saccharification améliorée d'une biomasse
WO2006125000A3 (fr) Procede de production du resveratrol dans un micro-organisme oleagineux de recombinaison
WO2011150241A8 (fr) Réduction du niveau de lactate et augmentation de la production de polypeptides par régulation négative de l'expression de la lactate déshydrogénase et de la pyruvate déshydrogénase kinase
WO2010006076A3 (fr) Procédés, compositions et systèmes pour une bioproduction biosynthétique de 1,4-butanediol
WO2006124999A3 (fr) Procede de production du resveratrol dans une cellule hote bacterienne recombinante
ZA201008795B (en) Production of butanediol by anaerobic microbial fermentation
MX2013010481A (es) Produccion microbiana de productos quimicos y composiciones, metodos y sistemas relacionados.
WO2011044279A3 (fr) Systèmes microbiens pour la production de produits chimiques d'usage courant
WO2009143455A3 (fr) Résistance manufacturée aux alcools aliphatiques
CA2797046A1 (fr) Micro-organismes et methodes de biosynthese de propylene
WO2009134964A3 (fr) Procédé de fermentation amélioré utilisant des mélasses
WO2009081012A3 (fr) Procedes de production d'acide succinique

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09805462

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 13057359

Country of ref document: US

122 Ep: pct application non-entry in european phase

Ref document number: 09805462

Country of ref document: EP

Kind code of ref document: A2

REG Reference to national code

Ref country code: BR

Ref legal event code: B01E

Ref document number: PI0917085

Country of ref document: BR

Free format text: IDENTIFIQUE O SIGNATARIO DAS PETICOES NO 016110000589 DE 04/02/2011 E 016110001632 DE 04/04/2011, UMA VEZ QUE NAO E POSSIVEL IDENTIFICAR O NOME DO RESPONSAVEL PELA ASSINATURA DOS FORMULARIOS, NAO SENDO POSSIVEL DETERMINAR SE ESTE FAZ PARTE DOS PROCURADORES ELENCADOS NA PROCURACAO E SE TEM PODERES PARA ATUAR EM NOME DO DEPOSITANTE, E O ARTIGO 216 DA LEI 9.279/1996 DE 14/05/1996 (LPI) DETERMINA QUE OS ATOS PREVISTOS NESTA LEI SERAO PRATICADOS PELAS PARTES OU POR SEUS PROCURADORES, DEVIDAMENTE QUALIFICADOS .

ENPW Started to enter national phase and was withdrawn or failed for other reasons

Ref document number: PI0917085

Country of ref document: BR