WO2009145573A3 - 아미노 말단의 메티오닌이 제거된 재조합 사람 변이 인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의 제조방법 - Google Patents

아미노 말단의 메티오닌이 제거된 재조합 사람 변이 인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의 제조방법 Download PDF

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Publication number
WO2009145573A3
WO2009145573A3 PCT/KR2009/002835 KR2009002835W WO2009145573A3 WO 2009145573 A3 WO2009145573 A3 WO 2009145573A3 KR 2009002835 W KR2009002835 W KR 2009002835W WO 2009145573 A3 WO2009145573 A3 WO 2009145573A3
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WO
WIPO (PCT)
Prior art keywords
interferon
methionine
preparation
escherichia coli
amino end
Prior art date
Application number
PCT/KR2009/002835
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English (en)
French (fr)
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WO2009145573A2 (ko
WO2009145573A9 (ko
Inventor
이상목
하종천
양재균
이정식
박봉환
Original Assignee
주식회사 바이오큐어팜
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 주식회사 바이오큐어팜 filed Critical 주식회사 바이오큐어팜
Publication of WO2009145573A2 publication Critical patent/WO2009145573A2/ko
Publication of WO2009145573A3 publication Critical patent/WO2009145573A3/ko
Publication of WO2009145573A9 publication Critical patent/WO2009145573A9/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 아미노 말단의 메티오닌이 제거된 재조합 사람 변이 인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의 제조방법에 관한 것으로, 상세하게는 재조합 사람 변이 인터페론-베타(rHu IFN-βSer17, IFN-β 1b) 유전자를 pET21a(+) 벡터에 삽입하여 재조합 발현벡터 pET21a(+)IFN-βSer17를 제조하고, 메티오닌 아미노펩티다제 유전자를 pSTV29 벡터에 삽입하여 재조합 발현벡터 pSTV29Map을 제조한 후, 이 두 재조합 발현벡터를 하나의 대장균(BL21(DE3))에 형질전환시켜 아미노 말단의 메티오닌이 제거된 재조합 사람 변이 인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의 제조방법에 관한 것이다.
PCT/KR2009/002835 2008-05-29 2009-05-28 아미노 말단의 메티오닌이 제거된 재조합 사람 변이 인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의 제조방법 WO2009145573A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0050272 2008-05-29
KR1020080050272A KR20090124204A (ko) 2008-05-29 2008-05-29 아미노 말단의 메티오닌이 제거된 재조합 사람 변이인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의제조방법

Publications (3)

Publication Number Publication Date
WO2009145573A2 WO2009145573A2 (ko) 2009-12-03
WO2009145573A3 true WO2009145573A3 (ko) 2010-03-04
WO2009145573A9 WO2009145573A9 (ko) 2010-05-20

Family

ID=41377795

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/002835 WO2009145573A2 (ko) 2008-05-29 2009-05-28 아미노 말단의 메티오닌이 제거된 재조합 사람 변이 인터페론-베타 단백질을 생산하는 재조합 대장균 및 이의 제조방법

Country Status (2)

Country Link
KR (1) KR20090124204A (ko)
WO (1) WO2009145573A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102367441B (zh) * 2011-06-20 2014-09-17 安徽安科生物工程（集团）股份有限公司 一种去甲硫氨酸重组人干扰素-α2b的制备方法
IN2014MN00935A (ko) * 2011-11-22 2015-04-24 Lupin Ltd

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (en) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
US5814485A (en) * 1995-06-06 1998-09-29 Chiron Corporation Production of interferon-β (IFN-β) in E. coli
US20050214899A1 (en) * 2004-03-29 2005-09-29 Academia Sinica Removal of N-terminal methionine from proteins by engineered methionine aminopeptidase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (en) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
US5814485A (en) * 1995-06-06 1998-09-29 Chiron Corporation Production of interferon-β (IFN-β) in E. coli
US20050214899A1 (en) * 2004-03-29 2005-09-29 Academia Sinica Removal of N-terminal methionine from proteins by engineered methionine aminopeptidase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEN-BASSAT, ARIE ET AL.: "Processing of the Initiation Methionine from Proteins: Properties of the Escherichia coli Methionine Aminopeptidase and Its Gene Structure", J BACTERIOLOGY, vol. 169, no. 2, February 1987 (1987-02-01), pages 751 - 757 *
SHEN, T.J. ET AL.: "Production of unmodified human adult hemoglobin in Escherichia coli", PROC. NATL. ACAD. SCI. USA, vol. 90, no. 17, 1 September 1993 (1993-09-01), pages 8108 - 8112 *
WANG, AIJUN ET AL.: "Processing of N-Terminal Unnatural Amino Acids in Recombinant Human Interferon- in Escherichia coli", CHEMBIOCHEM, vol. 9, no. 2, 20 December 2007 (2007-12-20), pages 324 - 330 *

Also Published As

Publication number Publication date
KR20090124204A (ko) 2009-12-03
WO2009145573A2 (ko) 2009-12-03
WO2009145573A9 (ko) 2010-05-20

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